CN108977492A - 一种酶解肽及在抗前列腺癌方面的应用 - Google Patents
一种酶解肽及在抗前列腺癌方面的应用 Download PDFInfo
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- CN108977492A CN108977492A CN201811047198.3A CN201811047198A CN108977492A CN 108977492 A CN108977492 A CN 108977492A CN 201811047198 A CN201811047198 A CN 201811047198A CN 108977492 A CN108977492 A CN 108977492A
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 84
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 75
- 206010060862 Prostate cancer Diseases 0.000 title abstract description 37
- 208000000236 Prostatic Neoplasms Diseases 0.000 title abstract description 37
- OEYQBKYISMRWQB-UHFFFAOYSA-N Santal Chemical compound C=1C(OC)=CC(O)=C(C2=O)C=1OC=C2C1=CC=C(O)C(O)=C1 OEYQBKYISMRWQB-UHFFFAOYSA-N 0.000 claims abstract description 108
- 239000004365 Protease Substances 0.000 claims abstract description 52
- 108090000526 Papain Proteins 0.000 claims abstract description 24
- 229940055729 papain Drugs 0.000 claims abstract description 24
- 235000019834 papain Nutrition 0.000 claims abstract description 24
- 201000011510 cancer Diseases 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 62
- 239000012153 distilled water Substances 0.000 claims description 52
- 239000000843 powder Substances 0.000 claims description 44
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 27
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 27
- 230000006920 protein precipitation Effects 0.000 claims description 24
- 206010028980 Neoplasm Diseases 0.000 claims description 18
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 108010016634 Seed Storage Proteins Proteins 0.000 claims description 16
- 238000000108 ultra-filtration Methods 0.000 claims description 16
- 239000006228 supernatant Substances 0.000 claims description 15
- 238000004090 dissolution Methods 0.000 claims description 13
- 239000000654 additive Substances 0.000 claims description 8
- 230000000996 additive effect Effects 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 8
- 230000005611 electricity Effects 0.000 claims description 8
- 238000000605 extraction Methods 0.000 claims description 8
- 230000007062 hydrolysis Effects 0.000 claims description 8
- 238000006460 hydrolysis reaction Methods 0.000 claims description 8
- 239000012535 impurity Substances 0.000 claims description 8
- 239000012528 membrane Substances 0.000 claims description 8
- 239000000758 substrate Substances 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 7
- 229940088598 enzyme Drugs 0.000 claims description 7
- 238000005119 centrifugation Methods 0.000 claims description 6
- 239000012467 final product Substances 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 230000001376 precipitating effect Effects 0.000 claims description 6
- 244000189799 Asimina triloba Species 0.000 claims description 5
- 235000006264 Asimina triloba Nutrition 0.000 claims description 5
- 235000009467 Carica papaya Nutrition 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 abstract description 31
- 108091005804 Peptidases Proteins 0.000 abstract description 28
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract description 28
- 235000019419 proteases Nutrition 0.000 abstract description 28
- 230000000694 effects Effects 0.000 abstract description 11
- 201000010099 disease Diseases 0.000 abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 7
- 239000002246 antineoplastic agent Substances 0.000 abstract 1
- 229940041181 antineoplastic drug Drugs 0.000 abstract 1
- 210000004995 male reproductive system Anatomy 0.000 abstract 1
- 238000000034 method Methods 0.000 description 6
- 230000036210 malignancy Effects 0.000 description 5
- 241000219112 Cucumis Species 0.000 description 4
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 4
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 240000000513 Santalum album Species 0.000 description 2
- 235000008632 Santalum album Nutrition 0.000 description 2
- 230000001186 cumulative effect Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 210000004209 hair Anatomy 0.000 description 2
- 230000008506 pathogenesis Effects 0.000 description 2
- 210000002229 urogenital system Anatomy 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 206010005003 Bladder cancer Diseases 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- 102000007066 Prostate-Specific Antigen Human genes 0.000 description 1
- 108010072866 Prostate-Specific Antigen Proteins 0.000 description 1
- 208000007097 Urinary Bladder Neoplasms Diseases 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 201000005202 lung cancer Diseases 0.000 description 1
- 208000020816 lung neoplasm Diseases 0.000 description 1
- 210000002307 prostate Anatomy 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 201000005112 urinary bladder cancer Diseases 0.000 description 1
- 208000029584 urinary system neoplasm Diseases 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/011—Hydrolysed proteins; Derivatives thereof from plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Veterinary Medicine (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Pharmacology & Pharmacy (AREA)
- Wood Science & Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Water Supply & Treatment (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Description
酶解肽 | IC50值(μg/mL) |
复合蛋白酶1000u~3000u酶解肽(复合1000~3000) | 9.52 |
复合蛋白酶3000u~5000u酶解肽(复合3000~5000) | >100 |
复合蛋白酶5000u~7000u酶解肽(复合5000~7000) | 8.78 |
复合蛋白酶7000u~10000u酶解肽(复合7000~10000) | >100 |
木瓜蛋白酶1000u~3000u酶解肽(木瓜1000~3000) | 11.45 |
木瓜蛋白酶3000u~5000u酶解肽(木瓜3000~5000) | 8.63 |
木瓜蛋白酶5000u~7000u酶解肽(木瓜5000~7000) | >100 |
木瓜蛋白酶7000u~10000u酶解肽(木瓜7000~10000) | 10.17 |
Claims (10)
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CN201811047198.3A CN108977492B (zh) | 2018-09-09 | 2018-09-09 | 一种酶解肽及在抗前列腺癌方面的应用 |
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CN201811047198.3A CN108977492B (zh) | 2018-09-09 | 2018-09-09 | 一种酶解肽及在抗前列腺癌方面的应用 |
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CN108977492A true CN108977492A (zh) | 2018-12-11 |
CN108977492B CN108977492B (zh) | 2021-11-09 |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1827773A (zh) * | 2006-03-28 | 2006-09-06 | 华南理工大学 | 用低次烟叶蛋白制备抑菌肽的方法 |
CN106834404A (zh) * | 2017-02-27 | 2017-06-13 | 南京九寿堂医药科技有限公司 | 一种酶解多肽及用于制备治疗肺腺癌药物的用途 |
CN106906269A (zh) * | 2017-02-27 | 2017-06-30 | 南京九寿堂医药科技有限公司 | 一种酶解多肽及用于抑制小细胞肺癌干细胞侵袭和转移的用途 |
-
2018
- 2018-09-09 CN CN201811047198.3A patent/CN108977492B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1827773A (zh) * | 2006-03-28 | 2006-09-06 | 华南理工大学 | 用低次烟叶蛋白制备抑菌肽的方法 |
CN106834404A (zh) * | 2017-02-27 | 2017-06-13 | 南京九寿堂医药科技有限公司 | 一种酶解多肽及用于制备治疗肺腺癌药物的用途 |
CN106906269A (zh) * | 2017-02-27 | 2017-06-30 | 南京九寿堂医药科技有限公司 | 一种酶解多肽及用于抑制小细胞肺癌干细胞侵袭和转移的用途 |
Non-Patent Citations (2)
Title |
---|
ABHEEPSA MISHRA等: "Identification and structural characterization of a new pro-apoptotic cyclic octapeptide cyclosaplin from somatic seedlings of Santalum album L.", 《PEPTIDES》 * |
HIREHALLY BASAVARAJEGOWDA MAHESH等: "Multi-Omics Driven Assembly and Annotation of the Sandalwood (Santalum album) Genome", 《PLANT PHYSIOLOGY》 * |
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Inventor after: Liu Fei Inventor after: Jiao Liying Inventor after: Lv Shicui Inventor after: Wang Zhaoli Inventor before: Jiao Liying Inventor before: Lv Shicui Inventor before: Wang Zhaoli |
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Effective date of registration: 20211014 Address after: 17 Panjiayuan Nanli, Chaoyang District, Beijing Applicant after: Liu Fei Address before: 223001 Room 201, 198 Hanhou Avenue, qingjiangpu District, Huai'an City, Jiangsu Province Applicant before: HUAI'AN YITAI BIOLOGICAL TECHNOLOGY Co.,Ltd. |
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