CN108967160A - A kind of Zengcheng honey chrysanthemum test tube seedling transplantation method - Google Patents
A kind of Zengcheng honey chrysanthemum test tube seedling transplantation method Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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Abstract
The invention discloses a kind of Zengcheng honey chrysanthemum test tube seedling transplantation methods, belong to field of plant growing technology.In order to improve the yield of Zengcheng honey chrysanthemum, maintain the uniformity of its continued propagation, Zengcheng honey chrysanthemum test tube seedling transplantation method of the present invention include: selection transplanting garden, grafting matrix disinfection, test tube seedling lure root culture and hardening, tissue-cultured seedling plant, cultivation management, to realize the high technical advantage of Zengcheng honey chrysanthemum high survival rate, sugar content, it is suitble to promote and apply during field planting.
Description
Technical field
The present invention relates to a kind of Zengcheng honey chrysanthemum test tube seedling transplantation methods, belong to field of plant growing technology.
Background technique
Sweet chrysanthemum is the kind that nectar is uniquely rich in spirea, picks after in full bloom, endures sunlight to the fullest extent and shine, and removes fragrance
It is strong outer, avoid the cold and cool of medicine chrysanthemum, pharmacological property " sweet ", and maintain medicine chrysanthemum clear liver and improve vision, the drug effect of wind dispelling eliminating evil.Sweet chrysanthemum has
Higher economic value.In recent years, it becomes a kind of important forest economy plant again, extensively in woodland, hillside plantation,
Bring huge economic benefit and ecological benefits.
China has the climatic characteristic of north subtropical and warm wet bands transition, makes a clear distinction between the four seasons, and illumination is sufficient, and plum rains are significant, drop
Rain is concentrated, and the easily flood of rain heat same season, easily drought, many years average evaporation capacity is 1114.0 millimeters, average about 220 days frost-free periods, annual
Rainfall is 915 millimeters, 15.2 DEG C of average temperature of the whole year；Soil be mainly yellowish soil, rice soil, rhogosol, rendzinas, moisture soil,
The types such as purple soil.Since sweet chrysanthemum originates in subtropical zone, belong to happiness temperature, happiness water crop, it is drought-resistant, easily plant, it is good to manage,
Not only can extensive agriculture, but also there is the value finely cultivated.From the point of view of the above Meteorological Data Analysis, China is located in yangtse-huaihe region, sun
Light is sufficient, makes a clear distinction between the four seasons, and frost-free period is long, and accumulated temperature is high, and soil, weather conditions are very suitable for sweet chrysanthemum growth.
At present since method for planting lacks systemic plantation, there are no realize scientic planting, the sweet chrysanthemum of different planting techniques
Yield difference it is larger, study the scientic planting method economic value with higher and realistic price of sweet chrysanthemum.
Lin Xiaoping etc. has delivered " Zengcheng honey chrysanthemum " tissue-cultured seedling organic farming administrative skill, utilizes " Zengcheng honey chrysanthemum " tissue culture detoxification
Seedling carries out artificial organic farming and sums up a set of " Zengcheng honey chrysanthemum " organic farming management by the research of many years cultivation technique
Technology: the environment of planting requirement, seedling selection, planting site selection, planting technology, field management technology, the techniques of pest control
Deng.But it is poor that it practices practicability, it is difficult to realize the large-scale planting of Zengcheng honey chrysanthemum.
Summary of the invention
In order to improve the yield of Zengcheng honey chrysanthemum, the uniformity of its continued propagation is maintained, honey chrysanthemum test tube seedling in Zengcheng of the present invention is moved
Plant method include: selection transplanting garden, grafting matrix disinfection, test tube seedling lure root culture and hardening, tissue-cultured seedling plant, cultivation management
And etc., to realize the high technical advantage of Zengcheng honey chrysanthemum high survival rate, sugar content, it is suitble to push away during field planting
Wide application.
The present invention is achieved through the following technical solutions above-mentioned technical effect:
A kind of Zengcheng honey chrysanthemum test tube seedling transplantation method, specifically include the following steps:
1) with selecting transplanting garden: selection topography is flat, surrounding is penetrating, illumination is sufficient, well-drained open field conduct
With transplanting garden, garden ground complement vinyl house or simple plastic greenhouse are being transplanted,
2) test tube seedling transplanting selection carries out in vinyl house or simple plastic greenhouse, and the grafting matrix is clean by 70%
Peat soil and 30% perlite be uniformly mixed so as to obtain, carried out disinfection using matrix disinfectant to matrix；Use the big cave cave in 54 holes
Disk container for plant growth loads matrix thereto and matrix is slightly compacted and is filled and led up；
3) Zengcheng honey chrysanthemum tissue-cultured seedling is carried out luring root culture and hardening in being planted in matrix, rooted seedling lures root culture and refining
The seedling time is 20~25d, and emergence when offspring to be generated reaches following quality standard: (1) leaf color is dark green, and the number of blade reaches 6 or more；
(2) plant height 5cm or more；(3) well developed root system；(4) growth is normal；
4) tissue-cultured seedling after cleaning and sterilizing is transplanted, when plant ensures that matrix covers the base portion of seedling, light with hand after plant
Pinching comes into full contact with the root of seedling with matrix, and drenches root water；
5) cultivation management after being transplanted, controls the humidity in greenhouse, illumination, moisture, temperature, ventilation, fertilizer are in charge of reason,
And actively prevent and treat pest and disease damage；It emerges when field planting seedling quality standard to be achieved, field planting seedling quality standard: (1) blade is full
Full, leaf color is dark green, unfolds, the number of blade reaches 6 or more；(2) stem thickness is strong, plant height 20cm or more；(3) well developed root system, plant are strong
Health, no disease and pests harm；(4) growth is normal, distortionless phenomenon.
In Zengcheng honey chrysanthemum test tube seedling transplantation method described above, the matrix sterilization method includes: by 0.2% Gao Meng
Sour potassium solution is added in mixed-matrix and is uniformly mixed, and standing is after 2-3 days every other day again with 500 times of fenaminosulf (or state's light naturally
Root spirit) solution drenches.
In Zengcheng honey chrysanthemum test tube seedling transplantation method described above, method for cleaning and disinfecting before tissue-cultured seedling is transplanted in the step 4)
Are as follows: the test tube seedling refined lightly is pressed from both sides out from culture bottle with tweezers, is put into the plastic tub got ready, equipped with clean water and cleans,
Until base portion noresidue culture medium；Tissue-cultured seedling after cleaning up takes out after impregnating 5min with 0.02% liquor potassic permanganate.
In Zengcheng honey chrysanthemum test tube seedling transplantation method described above, the root water be emerald green skin of bamboo B1 nutritional agents add carbendazim or
The mixed liquor of thiophanate-methyl obtains after diluting 300-500 times, it is preferable that the carbendazim or thiophanate-methyl and emerald green skin of bamboo B1
The volume ratio of nutritional agents is 2:3.
In Zengcheng honey chrysanthemum test tube seedling transplantation method described above, the cultivation management includes following management:
1) moisture management: after test tube seedling plantation in 4d, covering moisturizing using plastic film, control humidity 80~90%,
It is gradually opened film, ventilation reduces humidity, and 10 days round-the-clock openings, humidity are consistent with natural environment after plantation；
2) illumination management: the Zengcheng honey chrysanthemum seedling during transplanting must shade, and intensity of illumination should be the 70%- of ambient lighting
80%, the field 1-2 should gradually increase illumination before being out of the garden, until full exposure；
3) water management: the moisture of matrix need to continue to keep moisture state, and plant answered timely water drenching after 5 days, and when water drenching adopts
With the mode of spray；Water drenching water should be accomplished first few rear more.
Preferably, when water drenching, 7d guarantees matrix from holding moisture state at 1~3cm of surface before transplanting；After new root is grown,
Water drenching should ensure that whole matrix are wet；
4) temperature management: control greenhouse temperature is between 15~32 DEG C, and optimum growth temperature is 20~25 DEG C, is lower than 15 DEG C
When growth retardation, when being higher than 32 DEG C, not only growth is prolonged slow, and easily causes burn seedlings.Therefore, the environment temperature of test tube transplantation of seedlings is most
Between 20~30 DEG C, in this temperature, general 7 days seedlings start long new root, and terminal bud grows tall, 10 days can be complete for good control
Open film.It if method reaches this temperature requirement, also to control and reach 15~32 DEG C, otherwise test tube seedling transplanting will failure.
5) ventilating management: by the freshness of the humidity for adjustable transplanting environment of divulging information, temperature and air, be conducive to seedling
Growth and disease control.First 3 days generally transplanted, which do not rain, opens film ventilation, can start to open by day after 3 days, first
It is to the last round-the-clock to open completely gradually by the extension time first from night gradually by enlarged-area from the both ends of canopy.
6) fertilizer is in charge of reason: test tube seedling is transplanted first 7 days, can be mixed with 0.1% potassium dihydrogen phosphate and 500 times of emerald green skin of bamboo B1 nutritional agents
The foliage top dressing that conjunction progress is 2 times, every 3 days 1 time.After 7 days, when growing new root and starting to grow tall, it can start to drench to matrix and apply water
Fertilizer, liquid manure formula be expect potassium dihydrogen phosphate, urea, calcium sulfate, ammonium magnesium sulfate 1:1:0.5:0.5 ratio be made into total concentration and be
The aqueous solution of 3000ppm is applied, and every 7d application is primary.
7) prevention and control of plant diseases, pest control: test tube seedling is transplanted first 7 days, is sprayed within every 3 days fungicide 1 time, is generally available carbendazim, quite
The fungicide such as spirit, thiophanate methyl, fenaminosulf, state's light English sodium, guo guanggen spirit, are used alternatingly, and spray within every 7 days later once, 25
After it, can according to disease, a situation arises, in time spray prevent and cure diseases.Disease occurs, diseased plant should be removed in time, and every 2~3
Its spray is primary, and continuous spraying 2~3 times.Fungicide a variety of must be used interchangeably, and special circumstances a variety of can be used in mixed way (alkalinity
Medicine cannot be mixed with acid medicine) and jinggangmeisu can be added.Preferably, the fungicide is used alternatingly, selected from carbendazim,
Quite one of spirit, thiophanate methyl, fenaminosulf, state's light English sodium, guo guanggen spirit.
Preferably, the vinyl house have including Illumination adjusting system, damp condition system, Ventilation conditioning system,
Spray system；The simple plastic greenhouse can be with rain-proof, and easy ventilation, is able to carry out temperature adjustment, damping, water drenching, fertilising and disease at shading
The operation such as pest control.
Compared with prior art, the present invention the technical effect obtained are as follows:
The present invention is optimized the tissue-cultured seedling culture of Zengcheng honey chrysanthemum from multiple angles, has obtained the best training of tissue-cultured seedling
Educate condition.It is shown by effect experiment, the high survival rate of the obtained tissue-cultured seedling of the present invention, seedling growth is healthy and strong, is appropriate for
It promotes and applies, there is important economic significance.
Specific embodiment
The present invention is further described below by way of specific embodiment, but the embodiment does not limit the present invention in any way
The range of patent protection.
A kind of Zengcheng honey chrysanthemum test tube seedling transplantation method of embodiment 1 comprising following step:
1 selection transplanting garden:, selection topography is flat, surrounding is penetrating, illumination is sufficient, well-drained open field conduct
With transplanting garden.
2. having in transplanting garden ground complement vinyl house or simple plastic greenhouse, the vinyl house including illumination
Regulating system, damp condition system, Ventilation conditioning system, spray system；The simple plastic greenhouse can with rain-proof, easily ventilation,
Shading, the operation such as be able to carry out temperature adjustment, damping, water drenching, fertilising and the prevention and control of plant diseases, pest control
3 seedling mediums prepare
3.1 substrate preparations: the matrix as test tube seedling transplanting requires that clean, ventilation, draining is good and has certain guarantor
Water, fertilizer-preserving ability can add 30% perlite to be uniformly mixed with 70% clean peat soil and use.
The disinfection of 3.2 matrix: matrix disinfectant can use potassium permanganate and fenaminosulf (or the soil fungicides such as root-rot spirit).Specifically
Operating method is to be sprayed while stirring with 0.2% liquor potassic permanganate in mixed-matrix, moistens matrix uniformly and is
Degree is drenched and is used every other day with 500 times fenaminosulf (or guo guanggen spirit) solution again every other day after filling.
The filling of 3.3 matrix: the big cave hole tray (6 caves × 9 caves) in 54 holes can be used in container for plant growth, it is possible to use the ㎝ of 8 ㎝ × 10
The soft cup of plastics.Matrix must be slightly compacted and be filled and led up when loading matrix.
The culture and hardening of 4 rooted seedlings
4.1 Zengcheng honey chrysanthemum tissue-cultured seedling carry out luring root culture and hardening in vinyl house.Hardening should be according to " growing from weak to strong "
Principle rationally adjusts the intensity of illumination of rooted seedling culture and hardening, and avoid burning because light is too strong seedling or light is excessively weak makes to plant
Seedling undergrowth, and temperature, the humidity etc. of hardening are adjusted according to Changes in weather situation in time.
It is 20~25d that 4.2 rooted seedlings, which lure root culture and hardening time, and the specific time is depending on practical condition of culture.
4.3 rooted seedling quality standards: (1) leaf color is dark green, and the number of blade reaches 6 or more；(2) plant height 5cm or more；(3) root
System is flourishing；(4) growth is normal.
5 tissue-cultured seedling bottle outlets and cleaning
5.1 tissue-cultured seedling bottle outlets: the test tube seedling refined lightly pressed from both sides out from culture bottle with tweezers (attention tries not to hurt
Root), it is put into the plastic tub got ready, equipped with clean water.
The cleaning of 5.2 tissue-cultured seedling: it after rooted seedling is poured out culture vessel, is cleaned twice with clean water, base portion should be accomplished without residual
Culture medium is stayed, hurts root, injury seedling less as far as possible.
The disinfection of 6 tissue-cultured seedling
Tissue-cultured seedling after cleaning up takes out after impregnating 5min. with 0.02% liquor potassic permanganate, can go up sieve or on
Cup is planted.
The plantation of 7 tissue-cultured seedling
Tissue-cultured seedling after cleaning and sterilizing can transplant, and must be classified to seedling before plant, be generally divided into three-level (level-one,
Second level, three-level), and transplanted by different grade classifications.When plant, matrix should cover the base portion of seedling, cannot cover
Not so center portion can cause seedling to be rotted because water drenching is improper, can not plant too shallow, otherwise be unfavorable for the growth of seedling new root.It plants
It after plant, is gently pinched with hand, comes into full contact with the root of seedling with matrix, and drenched root water (root water is added using emerald green skin of bamboo B1 nutritional agents
Bacterium spirit (40% or thiophanate-methyl) 500 liquid)
8 test tube seedling cultivation managements
8.1 moisture managements: after test tube seedling plantation, it is necessary to cover moisturizing with plastic film.It is general just plant before 4d, it is wet in canopy
Degree must keep 80~90%, after gradually openable film, ventilation reduces humidity, when starting to take off film, generally at night, and first from
Both sides, after be gradually expanded and extend the time, round-the-clock can open within the 10th day, humidity is consistent with natural environment.
8.2 illumination managements: the Zengcheng honey chrysanthemum seedling of plant must shade (80%), and illumination is too strong, easily cause burn seedlings, and
Easily cause high temperature in canopy, high temperature and humidity, and also it is stuffy, it is easy for causing disease, Serious Mortality occurs in seedling.It should increase before being out of the garden
Intense light irradiation, until full exposure, more reform of nature condition when to be out of the garden to field planting, the crop field for being more advantageous to Zengcheng honey chrysanthemum is raw
It is long.
8.3 water managements: the moisture of matrix must remain that moisture state, moisture cannot be excessive, and otherwise seedling is easy to
It goes rotten, when stem matter surface is done, just with drenching by spraying.Under normal circumstances, first 5 days of rigid nursery transplant, since film covers, canopy is in
Compare air-tight state, what water drenching moisture evaporation seldom, does not have to, after 5 days, since film often opens aeration-cooling, at this moment base
The moisture of matter is easy evaporation, thus starts to be easy to dry out it is necessary to pay attention to observing, and when discovery is dry, timely water drenching, when water drenching must be adopted
With the mode of spray, never with tap directly against shower.Water drenching water should accomplish it is first few after it is more, guarantee preceding 7d matrix from surface
Moisture state (clutch wet hand with hand but anhydrous drip) is kept at 1~3cm, to promote the growth of root.After 10d, new root can be grown
Out, terminal bud starts to take out high, and seedling starts survival and growth, and water drenching should ensure that whole matrix are wet and (clutch wet hand with hand but without water droplet
Under).
8.4 temperature managements: the growth temperature of Zengcheng honey chrysanthemum is generally 15~32 DEG C, and optimum growth temperature is 20~25 DEG C,
Growth retardation when lower than 15 DEG C, when being higher than 32 DEG C, not only growth is prolonged slow, and easily causes burn seedlings.Therefore, the ring of test tube transplantation of seedlings
Border temperature is preferably controlled between 20~30 DEG C, and in this temperature, general 7 days seedlings start long new root, and terminal bud grows tall, and 10
It can open film completely.It if method reaches this temperature requirement, also to control and reach 15~32 DEG C, otherwise test tube seedling transplanting will lose
It loses.
8.5 ventilating managements: by ventilation, the freshness of the adjustable humidity for transplanting environment, temperature and air is conducive to
The growth of seedling and the control of disease.First 3 days generally transplanted, which do not rain, opens film ventilation, can start to open by day after 3 days,
It is to the last round-the-clock to open completely gradually by the extension time first from night gradually by enlarged-area first from the both ends of canopy.
8.6 fertilizer are in charge of reason: test tube seedling is transplanted first 7 days, and 0.1% potassium dihydrogen phosphate and 500 times of emerald green skin of bamboo B1 nutritional agents can be used
The foliage top dressing that mixing progress is 2 times, every 3 days 1 time.After 7 days, when growing new root and starting to grow tall, it can start to drench to matrix and apply
Liquid manure, liquid manure formula be expect potassium dihydrogen phosphate, urea, calcium sulfate, ammonium magnesium sulfate 1:1:0.5:0.5 ratio be made into total concentration and be
The aqueous solution of 3000ppm is applied, and every 7d application is primary.
8.7 prevention and control of plant diseases, pest control: test tube seedling is transplanted first 7 days, is sprayed within every 3 days fungicide 1 time, is generally available carbendazim, quite
The fungicide such as spirit, thiophanate methyl, fenaminosulf, state's light English sodium, guo guanggen spirit, are used alternatingly, and spray within every 7 days later once, 25
After it, can according to disease, a situation arises, in time spray prevent and cure diseases.Disease occurs, diseased plant should be removed in time, and every 2~3
Its spray is primary, and continuous spraying 2~3 times.Fungicide a variety of must be used interchangeably, and special circumstances a variety of can be used in mixed way (alkalinity
Medicine cannot be mixed with acid medicine) and jinggangmeisu can be added.
9 tissue-cultured seedling are out of the garden
Field planting seedling quality standard: (1) blade is full, and leaf color is dark green, unfolds, the number of blade reaches 6 or more；(2) stem
It is sturdy, plant height 20cm or more；(3) well developed root system, plant health, no disease and pests harm；(4) growth is normal, distortionless phenomenon.
Comparative example 1 conventionally cultivates Zengcheng honey chrysanthemum field planting seedling.
Comparative example 2
In addition to 8.6 fertilizer are in charge of in reason using common fertilizer, remaining is the same as embodiment 1.
Comparative example 3
Except in step 7 root water be kingfisher skin of bamboo B1 500 times of liquid of nutritional agents in addition to remaining with embodiment 1.
The identical Zengcheng honey chrysanthemum of nursery stock is planted on nursery of the soil fertility without significant difference, measures its survival rate,
It is divided into level-one seedling (healthy and strong) after growth 10d according to the healthy and strong degree of its nursery stock, second level seedling (general), (thin and weak, growth is slow for three-level seedling
Slowly).As a result as follows:
The survival rate of the honey chrysanthemum transplantation method in Zengcheng of the present invention of table 1 and the healthy and strong degree measurement of nursery stock
| Group | Survival rate | The healthy and strong degree of nursery stock |
| Embodiment 1 | 98.4% | Level-one seedling |
| Comparative example 1 | 82.5% | Second level seedling |
| Comparative example 2 | 75.2% | Three-level seedling |
| Comparative example 3 | 72.3% | Second level seedling |
As seen from the above table, using Zengcheng honey chrysanthemum transplantation method survival rate highest of the present invention, nursery stock is healthy and strong, with it
His group, which is compared, has significant difference.
Claims (10)
1. a kind of Zengcheng honey chrysanthemum test tube seedling transplantation method, specifically include the following steps:
1) with selecting transplanting garden: selection topography is flat, surrounding is penetrating, illumination is sufficient, and well-drained open field is as transplanting
Garden, in transplanting garden ground complement vinyl house or simple plastic greenhouse；
2) test tube seedling transplanting selection carries out in vinyl house or simple plastic greenhouse, and the grafting matrix is by 70% clean mud
Charcoal soil and 30% perlite are uniformly mixed so as to obtain, and are carried out disinfection using matrix disinfectant to matrix；Use the big hole plate seedling growth in 54 holes
Container loads matrix thereto and matrix is slightly compacted and is filled and led up；
3) Zengcheng honey chrysanthemum tissue-cultured seedling is planted in matrix and lure root culture and hardening, rooted seedling lures root culture and hardening time
For 20~25d, emergence when offspring to be generated reaches following quality standard: (1) leaf color is dark green, and the number of blade reaches 6 or more；(2) strain
High 5cm or more；(3) well developed root system；(4) growth is normal；
4) tissue-cultured seedling after cleaning and sterilizing is transplanted, when plant ensures that matrix covers the base portion of seedling, and gently being pinched after plant with hand makes
The root of seedling comes into full contact with matrix, and drenches root water；
5) cultivation management after being transplanted controls the humidity in greenhouse, illumination, moisture, temperature, ventilation, fertilizer are in charge of reason, and are accumulated
Pole prevents and treats pest and disease damage；It emerges when field planting seedling quality standard to be achieved, field planting seedling quality standard: (1) blade is full, leaf
Color is dark green, unfolds, the number of blade reaches 6 or more；(2) stem thickness is strong, plant height 20cm or more；(3) well developed root system, plant health, nothing
Pest and disease damage；(4) growth is normal, distortionless phenomenon.
2. a kind of Zengcheng honey chrysanthemum test tube seedling transplantation method according to claim 1, which is characterized in that the matrix disinfection
Method includes: that 0.2% liquor potassic permanganate is added in mixed-matrix to be uniformly mixed, after standing 2-3 days naturally every other day again
It is drenched with 500 times of fenaminosulfs or guo guanggen spirit solution.
3. a kind of Zengcheng honey chrysanthemum test tube seedling transplantation method according to claim 1, which is characterized in that group in the step 4)
Train method for cleaning and disinfecting before seedling is transplanted are as follows: the test tube seedling refined lightly is pressed from both sides out from culture bottle with tweezers, is put into and is got ready, is equipped with
It is cleaned in the plastic tub of clean water, until base portion noresidue culture medium；Tissue-cultured seedling after cleaning up with 0.02% permanganic acid
Potassium solution takes out after impregnating 5min.
4. a kind of Zengcheng honey chrysanthemum test tube seedling transplantation method according to claim 1, which is characterized in that the root water is
Emerald green skin of bamboo B1 nutritional agents obtains after adding the mixed liquor of carbendazim or thiophanate-methyl to dilute 300-500 times.
5. a kind of Zengcheng honey chrysanthemum test tube seedling transplantation method according to claim 1, which is characterized in that the carbendazim or
The volume ratio of thiophanate-methyl and emerald green skin of bamboo B1 nutritional agents is 2:3.
6. a kind of Zengcheng honey chrysanthemum test tube seedling transplantation method according to claim 1, which is characterized in that the cultivation management
Including managing as follows:
1) moisture management: covering moisturizing using plastic film in 4d after test tube seedling plantation, controls humidity 80~90%, gradually beats
Film is opened, ventilation reduces humidity, and 10 days round-the-clock openings, humidity are consistent with natural environment after plantation；
2) illumination management: the Zengcheng honey chrysanthemum seedling during transplanting must shade, and intensity of illumination should be the 70%- of ambient lighting
80%, illumination is gradually increased within 1-2 days before being out of the garden, until full exposure；
3) water management: the moisture of matrix need to continue to keep moisture state, timely water drenching after plant 5 days, using spray when water drenching
Mode；Water drenching water should be accomplished first few rear more；
4) temperature management: control greenhouse temperature is to open film completely within 10 days between 15~32 DEG C；
5) ventilating management: ventilation parameters transplant the freshness of the humidity of environment, temperature and air；
6) fertilizer is in charge of reason: test tube seedling is transplanted first 7 days, can be mixed into 0.1% potassium dihydrogen phosphate with 500 times of emerald green skin of bamboo B1 nutritional agents
2 foliage top dressing of row, every 3 days 1 time；Start to drench to matrix after 7 days and apply liquid manure, liquid manure formula is material potassium dihydrogen phosphate, urea, sulphur
Sour calcium, ammonium magnesium sulfate 1:1:0.5:0.5 ratio be made into the aqueous solution that total concentration is 3000ppm and apply, every 7d application is primary；
7) prevention and control of plant diseases, pest control: test tube seedling is transplanted first 7 days, is sprayed within every 3 days fungicide 1 time, is sprayed within every 7 days once, after 25 days later
According to disease a situation arises in time spray prevent and cure diseases；Disease occurs, diseased plant should be removed in time, and every 2~3 days sprays one
It is secondary, continuous spraying 2~3 times.
7. a kind of Zengcheng honey chrysanthemum test tube seedling transplantation method according to claim 6, which is characterized in that when water drenching, before plant
7d guarantees matrix from holding moisture state at 1~3cm of surface；After new root is grown, water drenching should ensure that whole matrix are wet.
8. a kind of Zengcheng honey chrysanthemum test tube seedling transplantation method according to claim 6, which is characterized in that the fungicide is handed over
For use, it is selected from carbendazim, quite one of spirit, thiophanate methyl, fenaminosulf, state's light English sodium, guo guanggen spirit.
9. a kind of Zengcheng honey chrysanthemum test tube seedling transplantation method according to claim 8, which is characterized in that add in the fungicide
Enter jinggangmeisu.
10. a kind of Zengcheng honey chrysanthemum test tube seedling transplantation method according to claim 1, which is characterized in that the plastics are big
Canopy has including Illumination adjusting system, damp condition system, Ventilation conditioning system, spray system；The simple plastic greenhouse can
With rain-proof, easy ventilation, shading are able to carry out temperature adjustment, damping, water drenching, fertilising and the prevention and control of plant diseases, pest control etc. and operate.
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