CN108934694A - A method of promoting young age growth of taxol - Google Patents
A method of promoting young age growth of taxol Download PDFInfo
- Publication number
- CN108934694A CN108934694A CN201810714923.1A CN201810714923A CN108934694A CN 108934694 A CN108934694 A CN 108934694A CN 201810714923 A CN201810714923 A CN 201810714923A CN 108934694 A CN108934694 A CN 108934694A
- Authority
- CN
- China
- Prior art keywords
- yew
- group
- northeast
- taxus
- taxus chinensis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Abstract
The present invention relates to a kind of methods of promotion young age southerm yew growth, and operating procedure is: (1) preparing the aqueous solution of certain density isoamyl alcohol；(2) young age southerm yew is poured with the aqueous solution of isoamyl alcohol.Method provided by the invention is cultivated suitable for young age southerm yew, can promote the growth of young age southerm yew.
Description
Technical field
The invention belongs to plant physioecologies and fitochemical studies field, and in particular to a kind of promotion young age Chinese yew
The method of growth.
Background technique
Taxus chinensis in northeast (Taxus cuspidata) and southerm yew (Taxus chinensis var.mairei) are equal
For national I grades of Top-rated protected wild plants.Taxus (Taxus) plant complete stool contains a kind of tricyclic diterpene compound-purple
China fir alcohol, and be height with fruit and blade content.In China, southerm yew and taxus chinensis in northeast are bigger red of cultivated area
Beans genus Taxus, because of the southerm yew of artificial cultivation (old snow close with their wild species chemical component with taxus chinensis in northeast
English, 2006), content of taxol quite (Yang Fengjian, 2010), so they are also a primary raw material tree for producing taxol
Kind.Taxol has significant anticancer activity, mainly to ovarian cancer resistance and breast cancer etc., need of the whole world to paclitaxel kind anti-cancer drugs product
It asks and increases year by year, taxol is anticancer drug in recent years most popular on international market, it is considered to be human future most has between 20 years
One of anticancer drug of effect.Due to there is no artificial synthesized product to may replace taxol at present, taxol must divide from Chinese yew
It is obtained from, purifying, thus wild red bean China fir resource faces immense pressure.Since Chinese yew become anticarcinogen material plant with
Come, wild resource distribution is in obvious decline trend.
Carry out Chinese yew artificial forest to cultivate, alleviates taxus resource crisis, it has also become taxol industry sustainable development
Crucial (Li Xinlei, 2010).When cultivating Chinese yew nursery stock, in the identical situation of Chinese yew nursery stock, by simple method
The growth for promoting young age Chinese yew will obtain one of Chinese yew strong sprout approach.
Isoamyl alcohol, also referred to as 3- methyl butanol, colourless liquid, there is offending smell.It is slightly soluble in water, is miscible in alcohol, ether
Equal organic solvents.It can be used for processing chemicals, essence, analytical reagent, and for industries such as organic synthesis, pharmacy.From mesh
From the point of view of preceding disclosed document, there is not yet being applied to the report of plant and then regulating plant growth using isoamyl alcohol as growth regulator
Road.
The present invention is intended to provide a kind of method for promoting young age Chinese yew growth of seedling by pouring isoamyl alcohol.
Summary of the invention
The method provided by the invention for promoting young age growth of taxol, which is characterized in that operate as follows:
(1) by isoamyl alcohol and distilled water or tap water, 1:10000~1:100000 is mixed by volume, obtains solution I；
(2) solution I obtained with step (1) pours the nursery for having planted Chinese yew, and the amount of application of solution I is 0.4~2
Rise/square metre.
Wherein, the plant height before being handled of Chinese yew nursery stock described in step (2) is 20 centimetres~120 centimetres.
At interval of 5 days, the sequence repetitive operation according to step (1) and step (2) was primary.It is poured 60~120 days away from first
Afterwards, stop pouring.The processing group Chinese yew nursery stock speed of growth is significantly faster than that the control group Chinese yew seedlings for not pouring isoamyl alcohol
Wood.
The Chinese yew refers to the two kinds of plant taxus chinensis in northeast (Taxus cuspidata) and southern red bean of Taxus
One of China fir (Taxus chinensis var.mairei).
Specific embodiment
The present invention is described further by embodiment below, but the present invention is not limited to any one in embodiment
It is a.
Embodiment 1
The present embodiment pours southerm yew (Taxus chinensis var.mairei) using isoamyl alcohol.South
Chinese yew is divided into 2 groups, i.e. processing group and control group, carries out different processing respectively.The processing group refers to the implementation present invention
The group of patented technology content；Control group patent creativeness and practicability and the group set up to illustrate the invention.2 groups of southern reds
The seeding row spacing of beans China fir is identical, i.e., spacing in the rows is 0.8 meter, and line-spacing is 1.0 meters；Plant height is close, i.e., plant height is 50~70 centimetres.
Following steps operation is carried out to the processing group southerm yew in the present embodiment:
(1) by isoamyl alcohol, 1:10000 is mixed by volume with distilled water, obtains solution I；
(2) solution I obtained with step (1) pours the nursery for having planted southerm yew, and the amount of application of solution I is
0.4 liter/square metre；
(3) at interval of 5 days, the sequence repetitive operation according to step (1) and step (2) is primary, after first pour 60 days,
Stop pouring.
Following steps operation is carried out to the control group southerm yew of the present embodiment: with the solution of distilled water alternate process group
I, remaining operation is the same as processing group.
Through to southerm yew plant before and after the processing plant height and ground diameter analyze, the results showed that processing group south
Chinese yew plant height and ground diameter increase separately 18.6% and 7.2%；Control group southerm yew increases separately 11.4% and 3.8%.
The relative growth rate of processing group southerm yew plant height and ground diameter is 1.63 and 1.89 times of processing group respectively, illustrates to implement this hair
The processing group southerm yew speed of growth of bright content is significantly faster than that the southerm yew for not implementing the content of present invention.
Embodiment 2
The present embodiment pours southerm yew (Taxus chinensis var.mairei) using isoamyl alcohol.South
Chinese yew is divided into 2 groups, i.e. processing group and control group, carries out different processing respectively.The processing group refers to the implementation present invention
The group of patented technology content；Control group patent creativeness and practicability and the group set up to illustrate the invention.2 groups of southern reds
The seeding row spacing of beans China fir is identical, i.e., spacing in the rows is 0.8 meter, and line-spacing is 1.0 meters；Plant height is close, i.e., plant height is 20~30 centimetres.
Following steps operation is carried out to the processing group southerm yew in the present embodiment:
(1) by isoamyl alcohol, 1:40000 is mixed by volume with tap water, obtains solution I；
(2) solution I obtained with step (1) pours the nursery for having planted southerm yew, and the amount of application of solution I is 1
Rise/square metre；
(3) at interval of 5 days, the sequence repetitive operation according to step (1) and step (2) is primary, after first pour 90 days,
Stop pouring.
Following steps operation is carried out to the control group southerm yew of the present embodiment: with the solution of distilled water alternate process group
I, remaining operation is the same as processing group.
Through to southerm yew plant before and after the processing plant height and ground diameter analyze, the results showed that processing group south
Chinese yew plant height and ground diameter increase separately 26.2% and 11.3%；Control group southerm yew increases separately 16.5% and 6.2%.
The relative growth rate of processing group southerm yew plant height and ground diameter is 1.59 and 1.82 times of processing group respectively, illustrates to implement this hair
The processing group southerm yew speed of growth of bright content is significantly faster than that the southerm yew for not implementing the content of present invention.
Embodiment 3
The present embodiment pours taxus chinensis in northeast (Taxus cuspidata) using isoamyl alcohol.Taxus chinensis in northeast is divided
It is 2 groups, i.e. processing group and control group, carries out different processing respectively.The processing group, which refers to, to be implemented in the invention patent technology
The group of appearance；Control group patent creativeness and practicability and the group set up to illustrate the invention.The plant of 2 groups of taxus chinensis in northeast
Away from identical, i.e., spacing in the rows is 0.8 meter, and line-spacing is 1.0 meters；Plant height is close, i.e., plant height is 40~60 centimetres.
Following steps operation is carried out to the processing group taxus chinensis in northeast in the present embodiment:
(1) by isoamyl alcohol, 1:60000 is mixed by volume with distilled water, obtains solution I；
(2) solution I obtained with step (1) pours the nursery for having planted taxus chinensis in northeast, and the amount of application of solution I is 1
Rise/square metre；
(3) at interval of 5 days, the sequence repetitive operation according to step (1) and step (2) is primary, after first pour 60 days,
Stop pouring.
Following steps operation is carried out to the control group taxus chinensis in northeast of the present embodiment: with the solution of distilled water alternate process group
I, remaining operation is the same as processing group.
Through to taxus chinensis in northeast plant before and after the processing plant height and ground diameter analyze, the results showed that processing group northeast
Chinese yew plant height and ground diameter increase separately 13.5% and 6.3%；Control group taxus chinensis in northeast increases separately 8.6% and 4.4%.Place
The relative growth rate of reason group taxus chinensis in northeast plant height and ground diameter is 1.57 and 1.43 times of processing group respectively, illustrates to implement the present invention
The processing group Growth of Taxus cuspidata speed of content is significantly faster than that the taxus chinensis in northeast for not implementing the content of present invention.
Embodiment 4
The present embodiment pours taxus chinensis in northeast (Taxus cuspidata) using isoamyl alcohol.Taxus chinensis in northeast is divided
It is 2 groups, i.e. processing group and control group, carries out different processing respectively.The processing group, which refers to, to be implemented in the invention patent technology
The group of appearance；Control group patent creativeness and practicability and the group set up to illustrate the invention.The plant of 2 groups of taxus chinensis in northeast
Away from identical, i.e., spacing in the rows is 0.8 meter, and line-spacing is 1.0 meters；Plant height is close, i.e., plant height is 100~120 centimetres.
Following steps operation is carried out to the processing group taxus chinensis in northeast in the present embodiment:
(1) by isoamyl alcohol, 1:100000 is mixed by volume with tap water, obtains solution I；
(2) solution I obtained with step (1) pours the nursery for having planted taxus chinensis in northeast, and the amount of application of solution I is 2
Rise/square metre；
(3) at interval of 5 days, the sequence repetitive operation according to step (1) and step (2) is primary, pours 120 days away from first
Afterwards, stop pouring.
Following steps operation is carried out to the control group taxus chinensis in northeast of the present embodiment: with the solution of distilled water alternate process group
I, remaining operation is the same as processing group.
Through to taxus chinensis in northeast plant before and after the processing plant height and ground diameter analyze, the results showed that processing group northeast
Chinese yew plant height and ground diameter increase separately 23.5% and 11.8%；Control group taxus chinensis in northeast increases separately 14.6% and 7.2%.
The relative growth rate of processing group taxus chinensis in northeast plant height and ground diameter is 1.61 and 1.64 times of processing group respectively, illustrates to implement this hair
The processing group Growth of Taxus cuspidata speed of bright content is significantly faster than that the taxus chinensis in northeast for not implementing the content of present invention.
Claims (2)
1. the present invention relates to a kind of methods for promoting young age growth of taxol, which is characterized in that operate as follows:
(1) by isoamyl alcohol and distilled water or tap water, 1:10000~1:100000 is mixed by volume, obtains solution I；
(2) solution I that is obtained with step (1) pours the nursery for having planted Chinese yew, and the amount of application of solution I is 0.4~2 liter/
Square metre.
2. according to the method for claim 1, wherein Chinese yew described in step (2) is 20 centimetres~120 centimetres of plant height
Taxus chinensis in northeast (Taxus cuspidata) or southerm yew (Taxus chinensis var.mairei) plant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810714923.1A CN108934694B (en) | 2018-07-03 | 2018-07-03 | Method for promoting growth of young taxus chinensis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810714923.1A CN108934694B (en) | 2018-07-03 | 2018-07-03 | Method for promoting growth of young taxus chinensis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108934694A true CN108934694A (en) | 2018-12-07 |
| CN108934694B CN108934694B (en) | 2020-08-28 |
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ID=64485115
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810714923.1A Active CN108934694B (en) | 2018-07-03 | 2018-07-03 | Method for promoting growth of young taxus chinensis |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1272479A (en) * | 2000-05-17 | 2000-11-08 | 黄耀普 | Liquid broad-spectrum foliage spray plant growth regulator |
| CN1388245A (en) * | 2002-06-21 | 2003-01-01 | 中山大学 | Method of raising taxol yield of south taxad |
| CN102860194A (en) * | 2011-07-07 | 2013-01-09 | 福建南方制药股份有限公司 | Planting method of taxus chinensis and application of 5-aminolevulinic acid |
| CN103461332A (en) * | 2013-09-17 | 2013-12-25 | 南京通泽农业科技有限公司 | Method for improving plant photosynthetic capability |
| CN103828619A (en) * | 2014-03-31 | 2014-06-04 | 东北林业大学 | Method for promoting growth of camptotheca acuminate and increasing content of camptothecin |
-
2018
- 2018-07-03 CN CN201810714923.1A patent/CN108934694B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1272479A (en) * | 2000-05-17 | 2000-11-08 | 黄耀普 | Liquid broad-spectrum foliage spray plant growth regulator |
| CN1388245A (en) * | 2002-06-21 | 2003-01-01 | 中山大学 | Method of raising taxol yield of south taxad |
| CN102860194A (en) * | 2011-07-07 | 2013-01-09 | 福建南方制药股份有限公司 | Planting method of taxus chinensis and application of 5-aminolevulinic acid |
| CN103461332A (en) * | 2013-09-17 | 2013-12-25 | 南京通泽农业科技有限公司 | Method for improving plant photosynthetic capability |
| CN103828619A (en) * | 2014-03-31 | 2014-06-04 | 东北林业大学 | Method for promoting growth of camptotheca acuminate and increasing content of camptothecin |
Non-Patent Citations (1)
| Title |
|---|
| NAZNIN H A等: "Analysis of Volatile Organic Compounds Emitted by Plant Growth-Promoting Fungus Phoma sp. GS8-3 for Growth Promotion Effects on Tobacco", 《MICROBES AND ENVIRONMENTS》 * |
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| CN108934694B (en) | 2020-08-28 |
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