CN103875327B - Camptotheca acuminata seed germination method - Google Patents
Camptotheca acuminata seed germination method Download PDFInfo
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- CN103875327B CN103875327B CN201410112433.6A CN201410112433A CN103875327B CN 103875327 B CN103875327 B CN 103875327B CN 201410112433 A CN201410112433 A CN 201410112433A CN 103875327 B CN103875327 B CN 103875327B
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Abstract
The invention relates to a camptotheca acuminata seed germination method which comprises the following steps: (1) preparing methanol extracting liquid of branches and leaves of taxus mairei, and concentrating the extracting liquid to dryness to obtain a solid matter; (2) preparing the solid matter into water suspension liquid; (3) soaking camptotheca acuminata seeds in the water suspension liquid; and (4) germinating the camptotheca acuminata seeds in a culture box for certain days before stopping germination. The camptotheca acuminata seeds obtained by the method provided by the invention are used for culturing camptotheca acuminata nursery seedlings, so that the seed utilization rate can be increased and the strong seedlings can be cultured.
Description
Technical field
The invention belongs to plant physioecology and fitochemical studies field, be specifically related to a kind of camptotheca seed germinating method.
Background technology
Camplotheca acuminata (
camptotheca acuminatadence) be country's II grade of Top-rated protected wild plants, its complete stool all containing a kind of indoles alkaloid-camptothecine, and with fruit and blade content for height.Camptothecine has remarkable antitumor activity, and the whole world increases year by year to the demand of camptothecin antitumor medicine, and existing market share is more than 1,000,000,000 dollars.Can substituted camptothecin owing to there is no Prof. Du Yucang product at present, camptothecine must be separated from camplotheca acuminata, purifying obtains, and thus wild camplotheca acuminata resource faces immense pressure.Since camplotheca acuminata becomes the material plant of anticarcinogen, the distribution of its wild resource is in obvious decline trend.
In order to alleviate camplotheca acuminata crisis of resource, needing badly and carrying out the cultivation of camplotheca acuminata forest plantation.When cultivating camplotheca acuminata nursery stock, after usually will first germinateing, then sow.The germination rate of plant seed is high, germination vigor strong, imply that and emerges soon and neatly, Miao Zhuan; If germination rate is high, germination vigor is weak, imply that uneven, weak seedling of emerging is many.Therefore, when camptotheca seed is identical, improve the germination rate of camptotheca seed by simple method and germination vigor will be one of acquisition camplotheca acuminata approach in strong sprout.Meanwhile, improve camptotheca seed germination rate and also can reduce nursery sowing quantity, reduce costs.The present invention aims to provide a kind of method improving camptotheca seed germination rate and germination vigor.
At present, promote that the method for plant seed germination comprises water seed soaking, organic-solvent-penetrate way, Osmoconditioning, mechanical treatment, Stratificated treatment, physical field process (electric field, magnetic field, ultrasonic, infrared rays) etc.Southern enqlish yew Classification system
taxus chinensis(Pilger) Rehd. is a kind of coniferous species of Ormosia.Method system provided by the invention utilizes southern enqlish yew branches and leaves extract to soak camptotheca seed to improve its germination rate and germination vigor, therefore all different from said method.
Summary of the invention
The method that camptotheca seed provided by the invention germinates, is characterized in that, operates as follows:
(1) by dry for southern enqlish yew leaf, pulverize and obtain powder I, methyl alcohol and powder I in mass ratio 5:1 ~ 10:1 mix, refluxing extraction 1 ~ 3 hour, filtration extract, filtrate is concentrated into dry, obtains solid content II;
(2) solid content II step (1) obtained and water in mass ratio 1:100 ~ 1:1000 mix, and ultrasonic process 5 ~ 10 minutes obtains solid content II suspension;
(3) camptotheca seed is soaked, soaking temperature 25 ~ 35 DEG C, soak time 12 ~ 36 hours with solid content II suspension that step (2) obtains;
(4) in culture dish, four layers of filter paper with water-wet are spread, to be placed on filter paper by the camptotheca seed after step (3) process, cover culture dish lid, culture dish is inserted in incubator and is germinateed, put into 9th ~ 12 days after incubator to culture dish, stop germinateing.
Carry out camptotheca seed germination by method provided by the invention, its germination rate and germination vigor all obviously increase, and meanwhile, the rush day of germinateing also can shift to an earlier date.
Embodiment
Below by embodiment, the present invention is described further.
Embodiment 1
In the present embodiment, camptotheca seed picks up from Sichuan.The operating procedure that camptotheca seed germinates is as follows:
(1) get southern enqlish yew branches and leaves dry, pulverize and obtain 490 grams of powder I, methyl alcohol and powder I in mass ratio 5:1 mix, refluxing extraction 2 hours, filtration extract, filtrate is concentrated into dry, obtains 82 grams of solid contents II;
(2) the 82 grams of solid contents II step (1) obtained and water in mass ratio 1:500 mix, and ultrasonic process 8 minutes obtains solid content II suspension;
(3) camptotheca seed is soaked, soaking temperature 25 DEG C, soak time 24 hours with solid content II suspension that step (2) obtains;
(4) in culture dish, four layers of filter paper with water-wet are spread, to be placed on filter paper by the camptotheca seed after step (3) process, cover culture dish lid, culture dish is inserted in incubator and is germinateed, put into the 9th day after incubator to culture dish, stop germinateing.
Replace solid content II suspension with distilled water, only carry out the step (3) in the present embodiment operating procedure and step (4), in this, as control treatment, carry out camptotheca seed germination.Statistic analysis result shows that the present embodiment camplotheca acuminata germinates the rush day for latter 6th day of seed soaking, and germination vigor is 58.6%, and germination rate is 79.6%; Contrast camplotheca acuminata germinates the rush day for latter 8th day of seed soaking, and germination vigor is 23.3%, and germination rate is 62.9%, and the method that the present embodiment provides is described, the germination vigor of camptotheca seed and germination rate all significantly improve, and the rush day of simultaneously germinateing also is advanced by 2 days.
Embodiment 2
In the present embodiment, camptotheca seed picks up from Guizhou.The operating procedure that camptotheca seed germinates is as follows:
(1) get southern enqlish yew branches and leaves dry, pulverize and obtain 260 grams of powder I, methyl alcohol and powder I in mass ratio 8:1 mix, refluxing extraction 3 hours, filtration extract, filtrate is concentrated into dry, obtains 46 grams of solid contents II;
(2) the 46 grams of solid contents II step (1) obtained and water in mass ratio 1:100 mix, and ultrasonic process 5 minutes obtains solid content II suspension;
(3) camptotheca seed is soaked, soaking temperature 35 DEG C, soak time 12 hours with solid content II suspension that step (2) obtains;
(4) in culture dish, four layers of filter paper with water-wet are spread, to be placed on filter paper by the camptotheca seed after step (3) process, cover culture dish lid, culture dish is inserted in incubator and is germinateed, put into the 10th day after incubator to culture dish, stop germinateing.
Replace solid content II suspension with distilled water, only carry out the step (3) in the present embodiment operating procedure and step (4), in this, as control treatment, carry out camptotheca seed germination.Statistic analysis result shows that the present embodiment camplotheca acuminata germinates the rush day for latter 7th day of seed soaking, and germination vigor is 59.2%, and germination rate is 81.3%; Contrast camplotheca acuminata germinates the rush day for latter 9th day of seed soaking, and germination vigor is 22.6%, and germination rate is 54.2%, and the method that the present embodiment provides is described, the germination vigor of camptotheca seed and germination rate all significantly improve, and the rush day of simultaneously germinateing also is advanced by 2 days.
Embodiment 3
In the present embodiment, camptotheca seed picks up from Guangxi.The operating procedure that camptotheca seed germinates is as follows:
(1) get southern enqlish yew branches and leaves dry, pulverize and obtain 320 grams of powder I, methyl alcohol and powder I in mass ratio 10:1 mix, refluxing extraction 1 hour, filtration extract, filtrate is concentrated into dry, obtains 61 grams of solid contents II;
(2) the 61 grams of solid contents II step (1) obtained and water in mass ratio 1:1000 mix, and ultrasonic process 10 minutes obtains solid content II suspension;
(3) camptotheca seed is soaked, soaking temperature 30 DEG C, soak time 36 hours with solid content II suspension that step (2) obtains;
(4) in culture dish, four layers of filter paper with water-wet are spread, to be placed on filter paper by the camptotheca seed after step (3) process, cover culture dish lid, culture dish is inserted in incubator and is germinateed, put into the 12nd day after incubator to culture dish, stop germinateing.
Replace solid content II suspension with distilled water, only carry out the step (3) in the present embodiment operating procedure and step (4), in this, as control treatment, carry out camptotheca seed germination.Statistic analysis result shows that the present embodiment camplotheca acuminata germinates the rush day for latter 6th day of seed soaking, and germination vigor is 52.2%, and germination rate is 89.6%; Contrast camplotheca acuminata germinates the rush day for latter 9th day of seed soaking, and germination vigor is 25.4%, and germination rate is 68.9%, and the method that the present embodiment provides is described, the germination vigor of camptotheca seed and germination rate all significantly improve, and the rush day of simultaneously germinateing also is advanced by 3 days.
Claims (1)
1. a camptotheca seed germinating method, is characterized in that, operates as follows:
(1) by dry for southern enqlish yew branches and leaves, pulverize and obtain powder I, methyl alcohol and powder I in mass ratio 5:1 ~ 10:1 mix, refluxing extraction 1 ~ 3 hour, filtration extract, filtrate is concentrated into dry, obtains solid content II;
(2) solid content II step (1) obtained and water in mass ratio 1:100 ~ 1:1000 mix, and ultrasonic process 5 ~ 10 minutes obtains solid content II suspension;
(3) camptotheca seed is soaked, soaking temperature 25 ~ 35 DEG C, soak time 12 ~ 36 hours with solid content II suspension that step (2) obtains;
(4) in culture dish, four layers of filter paper with water-wet are spread, to be placed on filter paper by the camptotheca seed after step (3) process, cover culture dish lid, culture dish is inserted in incubator and is germinateed, put into 9th ~ 12 days after incubator to culture dish, stop germinateing.
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| CN1183138C (en) * | 2002-09-11 | 2005-01-05 | 东北林业大学 | Process for promoting generation of 10-hydroxycamptothecin in the bud of camptotheca seed |
| CN1910994B (en) * | 2006-08-22 | 2010-07-28 | 东北林业大学 | Method for three-D cultivation of camptotheca acuminata, Chinese yew and rosemary |
| CN103430655B (en) * | 2012-09-10 | 2015-05-20 | 中国科学院华南植物园 | Method for increasing seed germination rate and survival rate of setaria plicata |
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