CN111493070B - Application of beta-myrcene in drought resistance and growth promotion of alfalfa - Google Patents
Application of beta-myrcene in drought resistance and growth promotion of alfalfa Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses an application of beta-myrcene in drought resistance and growth promotion of alfalfa, wherein beta-myrcene is prepared into a low-concentration aqueous solution by tween-20, and alfalfa seeds are cultured in a culture dish, and the result shows that the beta-myrcene remarkably promotes the growth of roots of alfalfa seedlings. Selecting beta-myrcene to prepare an aqueous solution by using Tween 20, and spraying the alfalfa seedlings under normal and drought treatments, wherein the results show that: the beta-myrcene has a promoting effect on the alfalfa under normal growth conditions, and has a more remarkable promoting effect on the alfalfa under drought conditions, so that the beta-myrcene has an obvious drought-resisting and growth-promoting effect on the alfalfa. The beta-myrcene is a natural product, can be degraded in nature, is non-toxic and harmless to the environment, can be prepared in large scale, has low cost, low use concentration, low dosage and strong effect, and has extremely high application potential.
Description
Technical Field
The invention relates to application of a compound to drought resistance and growth promotion of pasture, in particular to application of beta-myrcene to drought resistance and growth promotion of alfalfa.
Background
As an important forage grass, the country has listed 'alfalfa industrialization' as a scientific and technological attack and customs project to seek to realize the optimization of the planting industry structure in China and promote the sustainable development of agriculture. Alfalfa, as a high quality variety in alfalfa, is an important source of high quality protein in animal husbandry. Alfalfa is suitable for warm and semi-humid to semi-arid climates, and is mostly distributed in the north of Yangtze river in China. In areas with less rainfall, the alfalfa faces the problem of how to resist drought and increase yield, and the traditional method mainly promotes the growth of the root system of the alfalfa by coupling water and fertilizer so that the alfalfa can absorb more nutrients and water to realize yield increase and drought resistance. However, the use of large amounts of fertilizers can salinize and harden the soil in water-deficient areas, threaten the safety of the soil and underground water resources of the planting field, and affect the planting of the following alfalfa and other pasture grasses or crops. However, natural ingredients extracted from nature are increasingly regarded as a substitute for fertilizers because they can be degraded in nature and do not pollute soil and groundwater. The natural products with rich varieties, different configurations and various action mechanisms in the nature provide possibility for the natural products.
beta-Myrcene, the name of English, beta-Myrcene, the structure of which is shown in figure 1, is colorless or light yellow liquid and has light balsam fragrance. In the application of the beta-myrcene, the beta-myrcene is mostly used as a raw material to synthesize other substances or be used as an insect repellent, and the beta-myrcene has growth promoting and drought resisting effects on plants and is not reported at home and abroad. The compound can be artificially and chemically synthesized, and has been reported in a plurality of documents, so that the compound has the advantages of easily available sources, low price and promising development and utilization prospect.
Reference documents:
Osman Ciftci,M.Namik Oztanir,Asl1Cetin.Neuroprotective Effects ofβ-Myrcene Following Global Cerebral Ischemia/Reperfusion-Mediated Oxidative and Neuronal Damage in a C57BL/J6 Mouse[J].Neurochemical Research,2014,39(9).
Gul Baykalir Burcu，Ciftci Osman,Cetin Asl1,Oztanir Mustafa Namik,Basak Türkmen
The protective cardiac effects ofΒ-myrcene after global cerebral1schemia/reperfusion in C57BL/J6 mouse[J].Acta Cirurgica Brasileira,2016-07，31(7):456-462；
application research progress of lianjianwei, xiajianling, huangkun, populus cyamonia, wangmei and beta-myrcene in organic reaction [ J ] chemical engineering progress 2011, 30(08): 1826-;
synthesis and characterization of Liangjianwei, Huangkun, Wangma, Dong, Prunus mume, Xiajing, 4- (4-methyl-3-pentenyl) -4-cyclohexene-1, 2-dicarboxamide [ J ] chemical reagents, 2012,34(06): 505-vallisan 507;
the synthesis and structural analysis of new monoterpene derivatives from beta-myrcene in the world, Lindline, Zhangai, chemistry and adhesion, 2005(03) 131-;
yangming, study of the catalysis of the reaction of beta-myrcene with maleic anhydride-Diels-Alder by strongly acidic macroporous resins [ A ]. International Industrial Electronic Center, Hong Kong. proceedings of 2011 International Conference on Biomedicine and Engineering (ISBE 2011V 2) [ C ]. International Industrial Electronic Center, Hong Kong: society for the application of Intelligent information technology, 2011: 4;
royal gold e, zhuyunl, bear normal health, source of myrcene and its application in perfume chemistry [ J ]. Shandong chemical industry, 2011,40(03): 47-50;
plum cloud, dawn cloud, coke flare, chenxiducing, pengfa, use of beta-myrcene as a component of a striped rice borer female repellent and repellent [ P ]. beijing: CN109042647A, 2018-12-21;
the research progress of the chemical synthesis method of Yang, Yuyao, Yao Niang and myrcene [ J ] the chemical progress 2012,31(09): 2039-;
a preparation method of 3, 4-poly beta-myrcene [ P ] Jilin: CN104650270A, 2015-05-27'
Yanghong, prosperous and powerful hole, Zhangzheng, Yanjunfeng, Li qin, preparation method and device of beta-myrcene [ P ]. Yunnan: CN1073158,1993-06-16.
At present, the research aiming at the growth promotion and drought resistance of beta-myrcene on plants at home and abroad is still in a blank stage. The substance similar to plant hormone exists in various plants, belongs to a natural product, is volatile, can be degraded in the nature, is environment-friendly, can be artificially synthesized, can be prepared in large quantity, and has great commercial potential in animal husbandry production.
Disclosure of Invention
The invention aims to provide application of beta-myrcene in drought resistance and growth promotion of alfalfa, in a culture dish experiment, beta-myrcene is prepared into concentrations of 0.25 mu l/mL, 0.5 mu l/mL, 1 mu l/mL, 2 mu l/mL, 5 mu l/mL and 10 mu l/mL by using Tween 20, the promotion effects on roots of alfalfa seedlings are 79.95%, 61.98%, 64.46%, 56.25%, 46.09% and 26.04% respectively, and the promotion effects on stems of seedlings are 31.65%, 28.63%, 23.08%, 22.955%, 15.13% and 16.65% respectively; in a pot experiment, beta-myrcene is selected to be prepared into a water solution with Tween 20 at two concentrations of 0.1 mu l/mL and 0.25 mu l/mL, and alfalfa seedlings under normal and drought treatment are sprayed, and the results show that: the beta-myrcene has a promoting effect on the alfalfa under the normal growth condition, has a more remarkable promoting effect on the alfalfa under the drought condition, and has an obvious drought-resisting and growth-promoting effect on the alfalfa. The beta-myrcene is a natural product, can be degraded in nature, is non-toxic and harmless to the environment, can be prepared in large scale, has low cost, low use concentration, low dosage and strong effect, and has extremely high application potential.
The invention relates to application of beta-myrcene in drought resistance and growth promotion of alfalfa.
Beta-myrcene is prepared into 0.25 mu l/mL, 0.5 mu l/mL, 1 mu l/mL, 2 mu l/mL, 5 mu l/mL and 10 mu l/mL beta-myrcene aqueous solution by Tween 20, and alfalfa seeds are cultured in a culture dish.
Preparing beta-myrcene into 0.1 mu l/mL and 0.25 mu l/mL beta-myrcene aqueous solutions by using Tween 20, and spraying the beta-myrcene aqueous solutions 1 time every 3 days and 2-4 times in the evening when the alfalfa seedlings in the pot plant grow to 10 cm.
When the alfalfa seedlings under drought treatment grow to 10cm, treating beta-myrcene with 0.1 mu l/mL and 0.25 mu l/mL beta-myrcene water solutions prepared from Tween 20, spraying the beta-myrcene water solutions 1 time every 3 days, and spraying the beta-myrcene water solutions 2-4 times in the evening.
In a culture dish experiment, beta-myrcene is prepared into concentrations of 0.25 mu l/mL, 0.5 mu l/mL, 1 mu l/mL, 2 mu l/mL, 5 mu l/mL and 10 mu l/mL by Tween 20, the promotion effects on the roots of alfalfa seedlings are 79.95%, 61.98%, 64.46%, 56.25%, 46.09% and 26.04% respectively, and the promotion effects on the stems of seedlings are 31.65%, 28.63%, 23.08%, 22.955%, 15.13% and 16.65% respectively, because the experiment is carried out in a culture dish, the growth of alfalfa seedlings is limited, the promotion effect on the roots is obviously higher than that of the stems, and the highest value can reach 79.95%. In order to further verify the promoting effect of the beta-myrcene on the alfalfa, a potting experiment is carried out, two concentrations of 0.1 mu l/mL and 0.25 mu l/mL of beta-myrcene aqueous solution are selected, and the alfalfa seedlings under normal and drought treatment are sprayed, and the results show that: the beta-myrcene has a promoting effect on the alfalfa under the normal growth condition, has a more remarkable promoting effect on the alfalfa under the drought condition, and has an obvious drought-resisting and growth-promoting effect on the alfalfa.
The invention relates to an application of beta-myrcene in drought resistance and growth promotion of alfalfa, wherein the structural formula of the related beta-myrcene is shown as follows;
according to the application of the beta-myrcene in drought resistance and growth promotion of alfalfa, the purchased beta-myrcene standard substance is utilized, and the obvious promotion effect on the growth of seedlings of alfalfa can be achieved even under low concentration. In a greenhouse pot experiment, the beta-myrcene with the concentration of 0.1 mu g/mL and 0.25 mu g/mL has obvious promotion effect on the root length and the seedling height of the alfalfa, the promotion effect on the root can reach 62.03 percent under the conditions of 0.25 mu g/mL and spraying for 2 times, and the promotion effect on the seedling height can also reach 25.66 percent. Under the drought condition, the promotion effect of the beta-myrcene on the root length is particularly obvious, and the maximum value can reach 72.14 percent under the condition of spraying for 4 times at 0.1 mu g/mL. The root system of the plant is the key for the plant to absorb water and nutrient substances from the soil, and can completely show that the beta-myrcene sprayed alfalfa has huge growth potential and drought resistance. Meanwhile, under the drought condition, the biomass of the alfalfa is also obviously improved compared with a control without spraying beta-myrcene, the highest promotion effect of fresh substances can reach 70.95 percent, and the highest promotion effect of dry substances can reach 77.25 percent. As can be seen, the beta-myrcene, which is a compound extracted from natural plants, has obvious technical effects when used as a growth promoter and a drought-resistant agent of alfalfa.
The application of the beta-myrcene in drought resistance and growth promotion of alfalfa is disclosed, wherein the adopted beta-myrcene can be obtained from natural plant species or obtained by a chemical synthesis method, and related synthesis methods are reported. At present, the growth promoting and drought resisting effects of beta-myrcene on alfalfa are not reported at home and abroad.
The application of the beta-myrcene in drought resistance and growth promotion of alfalfa disclosed by the invention has the advantages that the used beta-myrcene exists in plants such as pepper, tea leaves and grapefruit, is a natural compound, has volatility, can be obtained by an artificial synthesis method, and cannot cause pollution to the environment.
According to the application of the beta-myrcene in drought resistance and growth promotion of alfalfa, the beta-myrcene can be prepared into water solutions with different concentrations and sprayed in a seedling stage to play a role in promoting growth.
The application of the beta-myrcene in drought resistance and growth promotion of alfalfa has the following beneficial effects:
1. the invention provides that the beta-myrcene can generate remarkable growth promotion effect on alfalfa seedlings at low concentration of 0.1 mu g/mL. The growth promoting effect is quite obvious in culture dishes and potted plants, 2 times of spraying and 4 times of spraying are respectively carried out on potted seedlings under the condition that the concentration is 0.1 mu g/mL, the growth promoting effect on the roots of alfalfa plants can reach 56.06% and 61.89%, and the growth promoting effect on the stems can reach 40.32% and 31.14%; when the concentration is 0.25 mu g/mL and the spraying treatment is carried out in the same way, the growth promoting effect on the roots of alfalfa plants can reach 62.03 percent and 59.93 percent, the growth promoting effect on the stems can reach 25.66 percent and 16.20 percent, and the biological promoting effect is not obvious. The beta-myrcene has a promoting effect on the growth of the alfalfa under the extremely low concentration. Under drought conditions, the growth promotion effect of the beta-myrcene is more obvious, and after the same concentration and treatment are carried out, the growth promotion effect on roots is 61.27 percent and 72.14 percent, and the growth promotion effect on stems is 61.38 percent and 42.87 percent. Meanwhile, under the drought condition, the beta-myrcene also has a remarkable promoting effect on the biomass of the alfalfa, the promoting effect on the fresh substances can reach 70.95%, 63.05%, 68.34% and 34.51%, and the promoting effect on the dry substances can reach 77.25%, 54.97%, 37.30% and 28.84%. Under the condition of low-concentration drought, the growth promotion effect of the beta-myrcene on the alfalfa is more obvious, the yield can be obviously improved, and the beta-myrcene has the growth promotion effect on the alfalfa and also has the obvious drought resistance effect.
2. The application of the beta-myrcene in drought resistance and growth promotion of alfalfa is suitable for being applied to fields to improve the yield and drought resistance of alfalfa. The main compound beta-myrcene related in the invention belongs to a natural compound, can be degraded in nature, does not cause pollution to the environment, and can be applied to planting of alfalfa. In addition, the dosage of the beta-myrcene is very small, the effect is obvious, and the mature synthesis method is adopted, so the source is wide, the cost performance is high, the solution can be prepared and sprayed in the actual operation, the operation is simple, the practicability is strong, and the commercial value is huge. The invention is characterized in that the beta-myrcene exists in various plants, is a natural product, has small dosage and strong effect, and can be degraded in nature, thereby causing no pollution to the environment.
Drawings
FIG. 1 is a graph showing the effect of beta-myrcene in a culture dish according to the present invention on the growth of alfalfa seedlings;
FIG. 2 is a graph showing the effect of beta-myrcene on the growth of the root length of potted alfalfa according to the present invention;
FIG. 3 shows the effect of beta-myrcene on the high growth of potted alfalfa seedlings;
FIG. 4 is a graph showing the effect of beta-myrcene on the fresh weight of potted alfalfa in accordance with the present invention;
FIG. 5 is a graph showing the effect of beta-myrcene in the present invention on the dry weight of potted alfalfa;
FIG. 6 shows alfalfa seedlings treated with control and beta-myrcene under drought conditions in potting experiments according to the present invention.
Detailed Description
The present invention will be described below by way of examples, but the present invention is not limited to the following examples.
All materials, reagents and equipment selected for use in the present invention are well known in the art, and other reagents and equipment known in the art may be suitable for use in the practice of the following embodiments of the present invention.
Example 1
The function of the beta-myrcene on promoting the growth of the alfalfa seedlings in the culture dish is as follows:
preparing beta-myrcene into a concentration of 10 mu l/mL by using Tween 20, and then sequentially diluting to 5 mu l/mL, 2 mu l/mL, 1 mu l/mL, 0.5 mu l/mL and 0.25 mu l/mL;
placing filter paper in a culture dish with the diameter of 9cm, adding 60 alfalfa seeds, then respectively adding 5mL of beta-myrcene solution with the concentration of 10 mu l/mL, 5 mu l/mL, 2 mu l/mL, 1 mu l/mL, 0.5 mu l/mL and 0.25 mu l/mL, repeatedly treating each for 2 times, and taking a blank control of distilled water; sealing with sealing film, culturing in 25 deg.C incubator in dark for 5 days, and measuring seedling height and root length;
the data statistical method comprises the following steps: firstly, the growth rate of the root length and the seedling height of the alfalfa to the blank contrast under each concentration is calculated as follows:
then detecting whether the difference among the groups of data of the growth rate is obvious or not by using the single-factor variance, analyzing the data by using an LSD (least squares decomposition) method, and marking the difference among the groups with different letters to be obvious, wherein the level is more than 0.05;
as a result: under the condition of the concentration of 0.25 mul/mL, the promotion effect of beta-myrcene on the roots of alfalfa seedlings can reach 79.95%, the effect is extremely obvious, the promotion effect on the stems can also reach 31.65%, the promotion effect of beta-myrcene on alfalfa is mainly shown on the roots, and meanwhile, the root system of a plant is the most main absorption part of substances such as water, mineral substances and the like in the growth and development process of the plant, so that the beta-myrcene can obviously promote the growth of alfalfa, and the yield of the alfalfa is further improved. Under other concentration conditions, the beta-myrcene also has remarkable promoting effects on the root length and the seedling height of alfalfa seedlings, the promoting effect on the root length is 61.98-26.04%, and the promoting effect on the seedling height is 28.63-15.13. See figure 1.
Example 2
The function of the beta-myrcene on promoting the growth of the potted alfalfa under the normal condition and the drought treatment condition is as follows:
preparing beta-myrcene into solutions with the concentrations of 0.1 mu l/mL and 0.25 mu l/mL by using Tween 20, filling the solutions into a small spray can for marking, and adding distilled water into another small spray can for blank control;
when the alfalfa seedlings in the pot plants grow to about 10cm, spraying prepared solutions with the concentrations of 0.1 mu l/mL and 0.25 mu l/mL, spraying for 1 time every 3 days, wherein each concentration is set to 2 spraying methods, namely spraying for 2 times and spraying for 4 times, dividing the pot plants into two groups, watering one group normally, watering the other group by half, and performing drought treatment by watering for half, measuring the root length, seedling height and fresh weight of the alfalfa 20 days after spraying the solutions, putting the fresh alfalfa into an oven to dry, and measuring the dry weight;
the data statistical method comprises the following steps: firstly, the growth rate of the root length and the seedling height of the alfalfa to the blank contrast under each concentration is calculated as follows:
then detecting whether the difference among the groups of data of the growth rate is obvious or not by using the single-factor variance, analyzing the data by using an LSD (least squares decomposition) method, and marking the difference among the groups with different letters to be obvious, wherein the level is more than 0.05; further, the fresh weight and dry weight growth rate was calculated using the following formula to represent the change in biomassAnd (3) conversion:
then detecting whether the difference among the groups of data of the growth rate is obvious or not by using the single-factor variance, analyzing the data by using an LSD (least squares decomposition) method, and marking the difference among the groups with different letters to be obvious, wherein the level is more than 0.05;
as a result: watering the prepared beta-myrcene solution for 2 times at the concentration of 0.1 mu l/mL and for 4 times at the concentration of 0.1 mu l/mL; the root length can be promoted by 56.06%, 61.89%, 62.03% and 59.93% under the normal watering treatment condition, and the seedling height can be promoted by 40.32%, 31.14%, 25.66% and 16.20% under the normal watering treatment condition;
under drought treatment conditions: the promotion effect on root length is 61.27%, 72.14%, 61.38% and 42.87%, and the promotion effect on seedling height is 28.83%, 16.21%, 14.52% and 2.12%; the promotion effect of beta-myrcene on the biomass of the alfalfa is more obvious under the drought condition than under the normal treatment condition, the fresh weight reaches 70.95%, 63.05%, 68.34% and 34.51%, and the dry weight reaches 77.25%, 54.97%, 37.30% and 28.84%, so that the beta-myrcene has the growth promotion effect on the alfalfa and also has the obvious drought resistance effect, and the effect is shown in the attached figures 2-6.
And (4) conclusion:
the beta-myrcene has a remarkable promoting effect on the growth of alfalfa seedlings, is very small in dosage, and can be considered as a plant growth regulator. The promoting effect on the root length of the alfalfa is particularly remarkable. As the root system is the main absorption tissue of substances such as water, mineral substances and the like in the growth and development process of plants, the beta-myrcene can obviously promote the growth of the alfalfa, and further improve the yield of the alfalfa. Under drought conditions, the beta-myrcene has obvious promotion effect on root length and biomass, so that the beta-myrcene can also be used as a drought-resistant agent for applying to alfalfa. In actual production, the dosage of the beta-myrcene is very small, the using method is simple, and the compound has a mature preparation method, so that the beta-myrcene has great commercial value in animal husbandry production.
Claims (3)
1. An application of beta-myrcene in drought resistance and growth promotion of alfalfa is characterized in that beta-myrcene is prepared into beta-myrcene aqueous solutions of 0.25 mul/mL, 0.5 mul/mL, 1 mul/mL, 2 mul/mL, 5 mul/mL and 10 mul/mL through Tween 20, and alfalfa seeds are cultured in a culture dish.
2. The application of beta-myrcene in drought resistance and growth promotion of alfalfa is characterized in that beta-myrcene is prepared into 0.1 mu l/mL and 0.25 mu l/mL of beta-myrcene aqueous solution by using Tween 20, when alfalfa seedlings in a pot plant grow to 10cm, the beta-myrcene aqueous solution is sprayed for 1 time every 3 days, and is sprayed for 2-4 times in the evening.
3. The application of beta-myrcene in drought resistance and growth promotion of alfalfa is characterized in that when alfalfa seedlings under drought treatment grow to 10cm, beta-myrcene is treated by a 0.1 mu l/mL and 0.25 mu l/mL beta-myrcene water solution prepared from Tween 20, the beta-myrcene water solution is sprayed for 1 time every 3 days, and the beta-myrcene water solution is sprayed for 2-4 times in the evening.
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