CN108934694B - Method for promoting growth of young taxus chinensis - Google Patents
Method for promoting growth of young taxus chinensis Download PDFInfo
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- CN108934694B CN108934694B CN201810714923.1A CN201810714923A CN108934694B CN 108934694 B CN108934694 B CN 108934694B CN 201810714923 A CN201810714923 A CN 201810714923A CN 108934694 B CN108934694 B CN 108934694B
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- mairei
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Biodiversity & Conservation Biology (AREA)
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- Wood Science & Technology (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a method for promoting growth of young taxus mairei, which comprises the following operation steps: (1) preparing an aqueous solution of isoamyl alcohol with a certain concentration; (2) irrigating young Taxus chinensis var mairei with isoamyl alcohol water solution. The method provided by the invention is suitable for cultivating the young taxus mairei and can promote the growth of the young taxus mairei.
Description
Technical Field
The invention belongs to the field of research on plant physiological ecology and phytochemistry, and particularly relates to a method for promoting growth of young taxus chinensis.
Background
Taxus cuspidata (Taxus subspidata) and Taxus mairei (Taxus chinensis var. mairei) are national class I important protection wild plants. The whole plant of Taxus (Taxus) contains taxol, a tricyclic diterpene compound, and has high fruit and leaf contents. In China, the taxus mairei and the taxus northeast are taxus plants with large planting areas, and because the artificially cultivated taxus mairei and taxus northeast are similar to the wild species in chemical components (chenxue ying, 2006) and equivalent in taxol content (Yang Jie Jian, 2010), the taxus mairei and the taxus northeast are also main raw material trees for producing taxol. Paclitaxel has significant anticancer activity, mainly against ovarian cancer, breast cancer and the like, and the global demand for paclitaxel anticancer drugs is increasing year by year, and paclitaxel is the most popular anticancer drug in the international market in recent years and is considered to be one of the most effective anticancer drugs in the future 20 years of human beings. Because no artificially synthesized product can replace paclitaxel at present, paclitaxel must be separated and purified from the taxus chinensis, and thus the wild taxus chinensis resources face huge pressure. Since the taxus chinensis becomes a raw material plant of an anticancer drug, the distribution of wild resources of the taxus chinensis shows a significantly decreasing trend.
The development of artificial forest cultivation of taxus chinensis and alleviation of resource crisis of taxus chinensis have become the key of sustainable development of paclitaxel industry (lisinopril, 2010). When the yew seedlings are cultivated, under the condition that the yew seedlings are the same, the method for promoting the growth of young yew by a simple method is one of the ways for obtaining yew strong seedlings.
Isoamyl alcohol, also known as 3-methyl butanol, is a colorless liquid with an unpleasant odor. Slightly soluble in water and miscible in organic solvents such as alcohol and ether. Can be used for photographic chemicals, essence, analytical reagent, and organic synthesis, pharmacy, etc. In view of the published literature, no report is found on the application of isoamyl alcohol as a growth regulator to plants to regulate the growth of the plants.
The invention aims to provide a method for promoting growth of young taxus chinensis seedlings by pouring isoamyl alcohol solution.
Disclosure of Invention
The method for promoting the growth of the young taxus chinensis is characterized by comprising the following steps:
(1) mixing isoamyl alcohol and distilled water or tap water according to the volume ratio of 1: 10000-1: 100000 to obtain a solution I;
(2) and (2) irrigating a seedling raising field with the solution I obtained in the step (1), wherein the application amount of the solution I is 0.4-2L/square meter.
Wherein the plant height of the Chinese yew seedlings in the step (2) is 20-120 cm before treatment.
Repeating the steps (1) and (2) once every 5 days. Stopping the irrigation 60-120 days after the initial irrigation. The growth speed of the treated group of the Chinese yew seedlings is obviously higher than that of the Chinese yew seedlings of a control group which is not watered with an isoprene solution.
The Taxus is one of two plants of Taxus genus Taxus northeast Taxus (Taxus cuspidata) and Taxus mairei (Taxus chinensis var.
Detailed Description
The present invention is further described below by way of examples, but the present invention is not limited to any one of the examples.
Example 1
This example uses isoamyl alcohol solution to irrigate Taxus mairei (Taxus chinensis var. The taxus mairei was divided into 2 groups, i.e., a treatment group and a control group, which were treated differently. The processing group refers to a group for implementing the technical content of the patent of the invention; the comparison group is a group established for explaining the inventive and practical purposes of the present invention. The plant spacing and the row spacing of the 2 groups of the taxus mairei are the same, namely the plant spacing is 0.8 m, and the row spacing is 1.0 m; the plant heights are close, namely the plant heights are all 50-70 cm.
The following steps were carried out on the treatment group taxus mairei in this example:
(1) mixing isoamyl alcohol and distilled water according to a volume ratio of 1: 10000 mixing to obtain a solution I;
(2) irrigating a seedling raising field in which the taxus mairei is planted with the solution I obtained in the step (1), wherein the application amount of the solution I is 0.4 liter per square meter;
(3) repeating the steps (1) and (2) once every 5 days, and stopping irrigation 60 days after the initial irrigation.
The following procedure was carried out on the control group taxus mairei of this example: the solution I of the treatment group was replaced with distilled water, and the same treatment group was followed.
The plant height and the ground diameter of the taxus mairei plants before and after treatment are analyzed, and the result shows that: the plant height and the ground diameter of the taxus mairei in the treated group are respectively increased by 18.6 percent and 7.2 percent; the increase of the control group of the taxus mairei is 11.4 percent and 3.8 percent respectively. The relative growth rates of the plant height and the ground diameter of the taxus mairei in the treatment group are respectively 1.63 and 1.89 times of those in the treatment group, which shows that the growth speed of the taxus mairei in the treatment group implemented by the content of the invention is obviously higher than that of the taxus mairei not implemented by the content of the invention.
Example 2
This example uses isoamyl alcohol solution to irrigate Taxus mairei (Taxus chinensis var. The taxus mairei was divided into 2 groups, i.e., a treatment group and a control group, which were treated differently. The processing group refers to a group for implementing the technical content of the patent of the invention; the comparison group is a group established for explaining the inventive and practical purposes of the present invention. The plant spacing and the row spacing of the 2 groups of the taxus mairei are the same, namely the plant spacing is 0.8 m, and the row spacing is 1.0 m; the plant heights are close, namely the plant heights are all 20-30 cm.
The following steps were carried out on the treatment group taxus mairei in this example:
(1) mixing isoamyl alcohol and tap water according to a volume ratio of 1: 40000 mixing to obtain solution I;
(2) irrigating a seedling raising field in which the taxus mairei is planted with the solution I obtained in the step (1), wherein the application amount of the solution I is 1 liter per square meter;
(3) repeating the steps (1) and (2) once every 5 days, and stopping the irrigation 90 days after the initial irrigation.
The following procedure was carried out on the control group taxus mairei of this example: the solution I of the treatment group was replaced with distilled water, and the same treatment group was followed.
The plant height and the ground diameter of the taxus mairei plants before and after treatment are analyzed, and the result shows that: the plant height and the ground diameter of the treated taxus mairei are respectively increased by 26.2 percent and 11.3 percent; the control group of taxus mairei increased by 16.5% and 6.2%, respectively. The relative growth rates of the plant height and the ground diameter of the taxus mairei in the treatment group are respectively 1.59 and 1.82 times of those of the treatment group, which shows that the growth speed of the taxus mairei in the treatment group implemented by the invention is obviously higher than that of the taxus mairei not implemented by the invention.
Example 3
This example uses isoamyl alcohol solution to irrigate Taxus cuspidata (Taxus cuspidata). The taxus cuspidata was divided into 2 groups, i.e., a treated group and a control group, which were treated differently. The processing group refers to a group for implementing the technical content of the patent of the invention; the comparison group is a group established for explaining the inventive and practical purposes of the present invention. The plant spacing and the row spacing of the 2 groups of taxus cuspidata are the same, namely the plant spacing is 0.8 m, and the row spacing is 1.0 m; the plant heights are close, namely the plant heights are all 40-60 cm.
The following steps of operation are carried out on the taxus cuspidata of the treatment group in the embodiment:
(1) mixing isoamyl alcohol and distilled water according to a volume ratio of 1: 60000 to obtain a solution I;
(2) irrigating a seedling raising field in which taxus cuspidata is planted with the solution I obtained in the step (1), wherein the application amount of the solution I is 1 liter per square meter;
(3) repeating the steps (1) and (2) once every 5 days, and stopping irrigation 60 days after the initial irrigation.
The following procedure was carried out on the taxus cuspidata of the control group of this example: the solution I of the treatment group was replaced with distilled water, and the same treatment group was followed.
The plant height and the ground diameter of the taxus cuspidata plants before and after treatment are analyzed, and the result shows that: the plant height and the ground diameter of the taxus cuspidata in the treatment group are respectively increased by 13.5 percent and 6.3 percent; the increase of the control group taxus cuspidata is 8.6% and 4.4% respectively. The relative growth rates of the plant height and the ground diameter of the taxus cuspidata in the treatment group are respectively 1.57 times and 1.43 times of those of the treatment group, which shows that the growth speed of the taxus cuspidata in the treatment group implementing the content of the invention is obviously higher than that of the taxus cuspidata in the absence of the content of the invention.
Example 4
This example uses isoamyl alcohol solution to irrigate Taxus cuspidata (Taxus cuspidata). The taxus cuspidata was divided into 2 groups, i.e., a treated group and a control group, which were treated differently. The processing group refers to a group for implementing the technical content of the patent of the invention; the comparison group is a group established for explaining the inventive and practical purposes of the present invention. The plant spacing and the row spacing of the 2 groups of taxus cuspidata are the same, namely the plant spacing is 0.8 m, and the row spacing is 1.0 m; the plant heights are close, namely the plant heights are all 100-120 cm.
The following steps of operation are carried out on the taxus cuspidata of the treatment group in the embodiment:
(1) mixing isoamyl alcohol and tap water according to a volume ratio of 1: 100000 to obtain a solution I;
(2) irrigating a seedling raising field in which taxus cuspidata is planted with the solution I obtained in the step (1), wherein the application amount of the solution I is 2 liters per square meter;
(3) repeating the steps (1) and (2) once every 5 days, and stopping irrigation 120 days after the initial irrigation.
The following procedure was carried out on the taxus cuspidata of the control group of this example: the solution I of the treatment group was replaced with distilled water, and the same treatment group was followed.
The plant height and the ground diameter of the taxus cuspidata plants before and after treatment are analyzed, and the result shows that: the plant height and the ground diameter of the taxus cuspidata in the treatment group are increased by 23.5 percent and 11.8 percent respectively; the increase of the control group taxus cuspidata is 14.6% and 7.2% respectively. The relative growth rates of the plant height and the ground diameter of the taxus cuspidata in the treatment group are respectively 1.61 times and 1.64 times of those of the treatment group, which shows that the growth speed of the taxus cuspidata in the treatment group implementing the content of the invention is obviously higher than that of the taxus cuspidata in the absence of the content of the invention.
Claims (1)
1. A method for promoting growth of young taxus chinensis is characterized by comprising the following steps:
(1) mixing isoamyl alcohol and distilled water or tap water according to the volume ratio of 1: 10000-1: 100000 to obtain a solution I;
(2) irrigating a seedling raising field with the solution I obtained in the step (1), wherein the application amount of the solution I is 0.4-2L/square meter;
wherein the Taxus chinensis in the step (2) is a Taxus cuspidata (Taxus cuspidata) or Taxus chinensis var. mairei plant with the plant height of 20-120 cm;
repeating the operation once according to the sequence of the step (1) and the step (2) every 5 days; stopping the irrigation 60-120 days after the initial irrigation.
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| CN201810714923.1A CN108934694B (en) | 2018-07-03 | 2018-07-03 | Method for promoting growth of young taxus chinensis |
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| CN201810714923.1A CN108934694B (en) | 2018-07-03 | 2018-07-03 | Method for promoting growth of young taxus chinensis |
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| CN108934694B true CN108934694B (en) | 2020-08-28 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1272479A (en) * | 2000-05-17 | 2000-11-08 | 黄耀普 | Liquid broad-spectrum foliage spray plant growth regulator |
| CN1388245A (en) * | 2002-06-21 | 2003-01-01 | 中山大学 | Method of raising taxol yield of south taxad |
| CN102860194A (en) * | 2011-07-07 | 2013-01-09 | 福建南方制药股份有限公司 | Planting method of taxus chinensis and application of 5-aminolevulinic acid |
| CN103461332A (en) * | 2013-09-17 | 2013-12-25 | 南京通泽农业科技有限公司 | Method for improving plant photosynthetic capability |
| CN103828619A (en) * | 2014-03-31 | 2014-06-04 | 东北林业大学 | Method for promoting growth of camptotheca acuminate and increasing content of camptothecin |
-
2018
- 2018-07-03 CN CN201810714923.1A patent/CN108934694B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1272479A (en) * | 2000-05-17 | 2000-11-08 | 黄耀普 | Liquid broad-spectrum foliage spray plant growth regulator |
| CN1388245A (en) * | 2002-06-21 | 2003-01-01 | 中山大学 | Method of raising taxol yield of south taxad |
| CN102860194A (en) * | 2011-07-07 | 2013-01-09 | 福建南方制药股份有限公司 | Planting method of taxus chinensis and application of 5-aminolevulinic acid |
| CN103461332A (en) * | 2013-09-17 | 2013-12-25 | 南京通泽农业科技有限公司 | Method for improving plant photosynthetic capability |
| CN103828619A (en) * | 2014-03-31 | 2014-06-04 | 东北林业大学 | Method for promoting growth of camptotheca acuminate and increasing content of camptothecin |
Non-Patent Citations (1)
| Title |
|---|
| Analysis of Volatile Organic Compounds Emitted by Plant Growth-Promoting Fungus Phoma sp. GS8-3 for Growth Promotion Effects on Tobacco;Naznin H A等;《Microbes and Environments》;20131231;第28卷(第1期);第42-49页 * |
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