CN108811838B

CN108811838B - Cucumber grafting method

Info

Publication number: CN108811838B
Application number: CN201810913436.8A
Authority: CN
Inventors: 于贤昌; 苗丽; 李衍素; 闫妍; 贺超兴
Original assignee: Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences
Current assignee: Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences
Priority date: 2018-08-13
Filing date: 2018-08-13
Publication date: 2020-05-26
Anticipated expiration: 2038-08-13
Also published as: CN108811838A

Abstract

The invention discloses a cucumber grafting method, which comprises the following steps: (1) cutting the cucumber seedling along the basal part of the hypocotyl to obtain an initial cucumber scion; (2) soaking the initial cucumber scions in the mixed solution for 15-20 min; (3) cutting the soaked initial cucumber scion 1-1.5cm below the growth point of the cucumber scion at 30-35 deg. along the direction perpendicular to the cotyledon growing direction, and cutting off the part below the initial cucumber scion to obtain cucumber scion; cutting upwards at an angle of 30-35 degrees at a position 1-1.5cm below the growth point of the pumpkin seedling along a direction parallel to the cotyledon growing direction, and completely cutting off the part above the pumpkin seedling to obtain a pumpkin stock; (4) and (4) rapidly and tightly sticking the cuts of the cucumber scions obtained in the step (3) and the cuts of the pumpkin rootstocks together, and fixing the cuts and the cuts by using a sleeve. The method is simple to operate, is suitable for mechanical batch production, and has short healing period of grafting wound and high survival rate.

Description

Cucumber grafting method

Technical Field

The invention belongs to the field of vegetable planting, and particularly relates to a cucumber grafting method.

Background

The grafting can not only keep the excellent characters of the scion variety, but also utilize the favorable characteristics of the stock to achieve the purposes of early fruiting and improving the stress resistance of plants to biotic and abiotic stresses (such as enhancing cold resistance, drought resistance, disease and insect resistance and the like) so as to improve the yield of crops and improve the quality of the crops, and in addition, can also economically utilize propagation materials to increase the number of seedlings and create novel ornamental plants and new species which are commonly used for the propagation of fruit trees, forest trees and flowers; can also be used for seedling culture of melon vegetables. In recent years, grafting has also been widely used in life sciences as a research system.

The cucumber/pumpkin grafting method mainly comprises grafting, inarching and root-cutting grafting methods, but the survival rate of grafted seedlings and the quality of the grafted seedlings are not high because the growth points of the rootstocks are not completely removed, scions generate adventitious roots in cavities of the rootstocks and hypocotyls of the rootstocks are excessively extended.

Chinese patent application CN106376358A discloses a cucumber grafting double-head coexistence seedling raising method, which reserves the growing point of a stock, does not remove the growing point of the stock, and allows the scion and the bud of the original stock to coexist and grow simultaneously in a period of time after grafting. The original growing point is not damaged and grows vigorously, nutrition is continuously provided for the original plant, the growing vigor is kept, the scion and the stock are completely fused, the stock growing point is removed after growth is recovered, the top advantage of the scion is kept, the scion continues to grow, the aim is to strengthen the seedling is achieved, the method has the disadvantages that two operations are needed after grafting, the stock growing point is removed at the later stage of grafting completion, the problem that the growing point is removed manually, and the removal of the growing point is not clean exists, and labor is consumed relatively. At present, the cucumber grafting in China mainly adopts manual methods of grafting, inarching and double root cutting, on one hand, the cost of the cucumber grafting is high, and on the other hand, the cucumber grafting method is complicated and is not suitable for mechanized production, so that the exploration of a relatively simple cucumber grafting method which is suitable for mechanized production and has high survival rate has important production significance.

Disclosure of Invention

The invention aims to overcome the defects that a cucumber grafting method in the prior art is complicated, is not suitable for mechanized production, and has long healing period of grafting wounds and low survival rate, and provides the cucumber grafting method. The method is simple to operate and suitable for mechanical batch production, and the grafting wound healing period is short and the survival rate is high.

In order to achieve the purpose, the invention provides a cucumber grafting method, which comprises the following steps:

(1) when the cotyledon of the cucumber seedling is completely flattened, the true leaf is just exposed, the cotyledon of the pumpkin seedling is just flattened, and the true leaf is not exposed, cutting the cucumber seedling along the basal part of the hypocotyl to obtain an initial cucumber scion;

(2) soaking the initial cucumber scion in a mixed solution containing 100-110mg/LIAA, 10-12mg/L6-BA and 20-25mg/LALA for 15-20 min;

(3) cutting the soaked initial cucumber scion 1-1.5cm below the growth point of the cucumber scion at 30-35 deg. along the direction perpendicular to the cotyledon growing direction, and cutting off the part below the initial cucumber scion to obtain cucumber scion; cutting upwards at an angle of 30-35 degrees at a position 1-1.5cm below the growth point of the pumpkin seedling along a direction parallel to the cotyledon growing direction, and completely cutting off the part above the pumpkin seedling to obtain a pumpkin stock;

(4) and (4) rapidly and tightly sticking the cuts of the cucumber scions obtained in the step (3) and the cuts of the pumpkin rootstocks together, and fixing the cuts and the cuts by using a sleeve.

The healing time of the method can be shortened to 6.22-6.83d, the efficiency during grafting reaches 91-100 plants/h, the seedling strengthening index reaches 0.78-0.92, and the survival rate reaches 92-98%. The method directly removes all the growth points and cotyledons of the rootstock, so that the problem of later-stage manual removal of residual growth points is solved, time and labor are saved, large-scale mechanical production can be carried out, excessive growth of hypocotyls of the rootstock is avoided, the operation is simple, and the grafting timeliness is improved; in addition, by controlling the grafting time of cucumber and pumpkin seedlings, the strong seedling index of the grafted seedlings can be improved, and the survival rate is further improved; the initial cucumber scion is soaked in the mixed solution containing 100-110mg/LIAA, 10-12mg/L6-BA and 20-25mg/LALA for 15-20min, so that the healing is promoted, the grafting healing time is shortened, the seedling strengthening index of the grafted seedling is improved, and the survival rate of the grafted seedling is improved.

Drawings

FIG. 1 is a schematic diagram of the grafting process of the present invention.

Detailed Description

The present invention will be described in detail below with reference to specific examples. These embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.

In the invention, IAA represents indole-3-acetic acid, 6-BA represents cytokinin, and ALA represents 5-aminolevulinic acid.

The invention provides a cucumber grafting method, which comprises the following steps:

According to the method disclosed by the invention, in the step (1), the cucumber can be sown 3-4 days earlier than the pumpkin.

According to the method disclosed by the invention, in the step (1), generally, the cotyledons of the cucumber seedlings are completely flattened, and the time for exposing the true leaves is 6-9 days after sowing. The cotyledon of the pumpkin seedling is just flattened, and the time when the true leaf is not exposed is 5-6 days after sowing.

According to the method of the present invention, the method further preferably comprises: before the step (1), performing moisture control on cucumber seedlings by maintaining the soil humidity at 50-60%; and normal water management is carried out on the pumpkin seedlings, so that the hypocotyl diameter of the cucumber seedlings can be improved, the diameter difference with the pumpkin is reduced, and the pumpkin seedlings can be better attached to the hypocotyls of the pumpkin.

According to the method of the present invention, preferably, the solvent of the mixed solution containing 100-110mg/LIAA, 10-12mg/L6-BA and 20-25mg/LALA is neutral oxidation potential water. The inventor creatively applies the neutral oxidation potential water to the agricultural field, and can properly improve the opening degree of the surface cell pores and the permeability of the cell walls of the cucumber seedlings, so that IAA, 6-BA and ALA in the mixed solution can be more stably and more continuously absorbed by the cucumber seedlings to be grafted, the grafting fusion time can be shortened after grafting, and the growth condition (such as the strong seedling index) of the grafted seedlings can be further improved to obviously improve the grafting survival rate.

According to the method of the present invention, preferably, the method further comprises: and respectively spraying trace element foliar fertilizer solutions to the surfaces of the cucumber scions once at the 3 rd and 5 th days after grafting, and spraying trace element foliar fertilizers through external sources, so that the requirement of scion growth in the healing period of a grafting interface is met, a foundation is provided for achieving high-quality strong seedlings at the later stage, and the growth condition (such as strong seedling index) of the grafted seedlings can be further improved while the grafting fusion time is shortened after grafting, so that the grafting survival rate is remarkably improved.

According to the method, the total weight of the trace element foliar fertilizer solution is taken as a reference, and the trace element foliar fertilizer solution contains 35-40% of choline chloride, 5-6% of diethyl aminoethyl hexanoate, 10-12% of zinc sulfate heptahydrate, 15-18% of cellulose, 0.01-0.05% of brassinolide and the balance of neutral oxidation potential water. In a similar way, the neutral oxidation potential water can properly improve the opening degree of cell pores on the surface layer of the cucumber scion and the permeability of the cell wall, so that the cucumber scion can more effectively absorb effective components in the trace element foliar fertilizer, the grafting fusion time can be shortened after grafting, the growth condition (such as strong seedling index) of the grafted seedling can be further improved to obviously improve the grafting survival rate, and the neutral oxidation potential water can improve the stability of choline chloride in the trace element foliar fertilizer solution. And choline chloride, diethyl aminoethyl hexanoate, zinc sulfate heptahydrate and brassinolide in the trace element foliar fertilizer solution can provide effective nutrients for the cucumber scions which are just injured.

According to the method, the preparation method of the trace element foliar fertilizer solution can comprise the following steps: according to the weight ratio of the components, choline chloride and neutral oxidation potential water are mixed and stirred uniformly, then fresh amine grease, zinc sulfate heptahydrate, cellulose and brassinolide are added into the mixed solution and stirred uniformly, and impurities are removed by filtration after precipitation.

According to the method of the present invention, preferably, an average of 1-5ml per plant is sprayed.

According to the method, the neutral oxidation potential water can be various conventional neutral oxidation potential water in the field, and preferably, the oxidation-reduction potential of the neutral oxidation potential water is between 800-1000, the free chlorine content is 250-950mg/L, and the pH value is 6.7-7.3, so that the functions of trace element foliar fertilizer and various growth hormones can be better promoted, and the healing of the grafting wound is promoted.

According to the method provided by the invention, in the step (3), the beveling angle of the cucumber scion is preferably the same as that of the pumpkin rootstock, so that the subsequent grafting and casing fixing are facilitated.

According to the method of the present invention, preferably, the method further comprises post-grafting temperature management, which comprises: the day temperature is 28-30 ℃/night temperature is 18-20 ℃.

According to the method of the present invention, preferably, the method further comprises post-engrafting illumination management, which comprises: keeping away from light 2-3d after grafting, and then using light intensity of 100-^-2s^-1The weak light irradiates for 3-5 days, and the 6 th-9 th days enter normal illumination management, so that the normal photosynthesis of the plant which is just grafted can be gradually recovered, the fatal damage to the grafted seedling caused by early exposure to the strong light is prevented, and the survival rate is further obviously influenced.

According to the method of the present invention, preferably, the method further comprises post-grafting moisture management, which comprises: the humidity in 7 days after grafting is maintained at 80-95%, the humidity in 8-10 days is reduced to 60%, the humidity management after grafting is crucial, the humidity is low, the healing of the wound of the grafted seedling is not facilitated, but the pathogen is easy to grow at the wound with too high humidity.

According to the method of the present invention, preferably, the method further comprises post-grafting ventilation management, which comprises: ventilating for 2 hours in the morning and at night 4-8 days after grafting, increasing the ventilation time to 3 hours in the morning and at night 9-12 days after grafting, and normally managing after 13 days. The morning and evening in the invention respectively means between 7 and 10 am and between 4 and 7 pm.

According to the method, the cucumber and the pumpkin can be various conventional varieties in the field, for example, the cucumber scion can adopt 'Zhongnong No. 26' and 'Zhongnong No. 116', the pumpkin stock adopts 'Jingxin stock No. 5', and 'Japanese rattan wood F1'. The cucumber and pumpkin varieties adopted in the test of the invention are commercially available. Various other materials that may be used in the present invention are commercially available unless otherwise specified.

Examples

IAA, 6-BA and ALA are all available from sigma.

Neutral oxidation potential water was prepared by the INNOWATECH apparatus available from yowa xuan environmental protection technologies ltd. The oxidation-reduction potential is 800, the free chlorine content is 250mg/L, and the pH value is 6.7.

Preparation example 1

Used for explaining the trace element foliar fertilizer solution prepared by the invention.

According to the weight ratio of the components (based on the total weight of the trace element foliar fertilizer solution, the trace element foliar fertilizer solution contains 35% of choline chloride, 5% of amine fresh grease, 10% of zinc sulfate heptahydrate, 15% of cellulose, 0.05% of brassinolide and the balance of neutral oxidation potential water), the choline chloride and the neutral oxidation potential water are mixed and stirred uniformly, then the amine fresh grease, the zinc sulfate heptahydrate, the cellulose and the brassinolide are added into the mixed solution and stirred uniformly, and the mixed solution is precipitated and filtered to remove impurities.

Preparation example 2

A trace-element foliar fertilizer solution was prepared according to the method of preparation example 1, except that neutral oxidation potential water was replaced with distilled water.

Example 1

This example serves to illustrate the cucumber grafting method of the present invention.

(1) As shown in figure 1, selecting 'Jingxin anvil No. 5' pumpkin as a rootstock, and 'Zhongnong No. 26' cucumber as a scion, wherein the pumpkin is sowed for 3 days later than the cucumber; when the cucumber cotyledon is completely flattened, the true leaf is just exposed (8 days after sowing), the pumpkin cotyledon is just flattened, and the true leaf is not exposed (5 days after sowing), cutting the cucumber seedling along the basal part of the hypocotyl to obtain an initial cucumber scion;

(2) soaking an initial cucumber scion in a mixed solution containing 100mg/LIAA, 10mg/L6-BA and 20mg/LALA for 15min, wherein the solvent of the mixed solution is neutral oxidation potential water;

(3) cutting the soaked initial cucumber scion 1cm below the growth point of the cucumber scion at a downward inclination angle of 30 degrees along the direction vertical to the cotyledon growing direction, and completely cutting off the part below the initial cucumber scion to obtain the cucumber scion; cutting upwards at an angle of 30 degrees along a cotyledon growing direction at a position 1cm below a growth point of the pumpkin seedling, and completely cutting off the part above the pumpkin seedling to obtain a pumpkin stock;

(4) quickly and tightly adhering the cuts of the cucumber scions obtained in the step (3) and the cuts of the pumpkin stocks together, and fixing the cuts and the cuts by using a sleeve;

(5) respectively spraying the trace element foliar fertilizer solution prepared in the preparation example 1 to the surfaces of the cucumber scions once at the 3 rd and 5 th days after grafting, and spraying 1ml of the trace element foliar fertilizer solution on each plant averagely every time; the temperature management after grafting is as follows: day temperature is 30 ℃/night temperature is 18 ℃; the lighting management after grafting is as follows: avoiding light within 2d after grafting, and then using light intensity of 100 mu mol m^-2s^-1The 8 th day enters normal illumination management after the 5d low-light illumination; the humidity management after grafting is as follows: after grafting, the humidity is maintained at 95% within 7d, and the humidity is reduced to 60% in 8 th-10 th d; the ventilation management after grafting is as follows: ventilating for 2 hours in the morning and at night 4-8 days after grafting, increasing the ventilation time to 3 hours in the morning and at night 9-12 days after grafting, and normally managing after 13 days.

Wherein, before step (1), cucumber seedlings are subjected to moisture control by maintaining the soil humidity at 50%; and normal water management is carried out on the pumpkin seedlings.

Example 2

(1) As shown in figure 1, Japanese celtis sinensis F1 pumpkin is selected as a rootstock, Zhongnong 116 cucumber is selected as a scion, and the pumpkin is sowed for 3 days later than the cucumber; when the cucumber cotyledon is completely flattened, the true leaf is just exposed (8 days after sowing), the pumpkin cotyledon is just flattened, and the true leaf is not exposed (5 days after sowing), cutting the cucumber seedling along the basal part of the hypocotyl to obtain an initial cucumber scion;

(2) soaking an initial cucumber scion in a mixed solution containing 110mg/LIAA, 12mg/L6-BA and 25mg/LALA for 18min, wherein the solvent of the mixed solution is neutral oxidation potential water;

(3) cutting the soaked initial cucumber scion 1.2cm below the growth point of the cucumber scion at a downward inclination angle of 32 degrees along the direction vertical to the cotyledon growing direction, and completely cutting off the part below the initial cucumber scion to obtain the cucumber scion; cutting upwards at an angle of 32 degrees along a direction parallel to the cotyledon growing direction at a position 1.2cm below the growing point of the pumpkin seedling, and completely cutting off the part above the pumpkin seedling to obtain a pumpkin stock;

(5) respectively spraying the trace element foliar fertilizer solution prepared in the preparation example 1 to the surfaces of the cucumber scions once at the 3 rd and 5 th days after grafting, and spraying 2ml of the trace element foliar fertilizer solution on each plant averagely each time; the temperature management after grafting is as follows: day temperature 28 ℃/night temperature 18 ℃; the lighting management after grafting is as follows: avoiding light within 3d after grafting, and then using light intensity of 120 mu mol m^-2s^-1The 3d is irradiated by weak light, and the 7d enters normal illumination management; the humidity management after grafting is as follows: after grafting, the humidity is maintained at 85% within 7d, and the humidity is reduced to 60% in 8 th-10 th d; the ventilation management after grafting is as follows: ventilating for 2 hours in the morning and at night 4-8 days after grafting, increasing the ventilation time to 3 hours in the morning and at night 9-12 days after grafting, and normally managing after 13 days.

Wherein, before step (1), cucumber seedlings are subjected to moisture control by maintaining the soil humidity at 55%; and normal water management is carried out on the pumpkin seedlings.

Example 3

(2) soaking an initial cucumber scion in a mixed solution containing 100mg/LIAA, 12mg/L6-BA and 25mg/LALA for 20min, wherein the solvent of the mixed solution is neutral oxidation potential water;

(3) cutting the soaked initial cucumber scion 1.5cm below the growth point of the cucumber scion at 35 ° downward along the direction perpendicular to the cotyledon growing direction, and cutting off the part below the initial cucumber scion to obtain the cucumber scion; cutting upwards at 35 degrees along the cotyledon growing direction at a position 1.5cm below the growth point of the pumpkin seedling, and completely cutting off the part above the pumpkin seedling to obtain a pumpkin stock;

(5) respectively spraying the trace element foliar fertilizer solution prepared in the preparation example 1 to the surfaces of the cucumber scions once at the 3 rd and 5 th days after grafting, and spraying 2ml of the trace element foliar fertilizer solution on each plant averagely each time; the temperature management after grafting is as follows: day temperature 28 ℃/night temperature 18 ℃; the lighting management after grafting is as follows: avoiding light within 3d after grafting, and then using light intensity of 100 mu mol m^-2s^-1The 3d is irradiated by weak light, and the 7d enters normal illumination management; the humidity management after grafting is as follows: after grafting, the humidity is maintained at 85% within 7d, and the humidity is reduced to 60% in 8 th-10 th d; the ventilation management after grafting is as follows: ventilating for 2 hours in the morning and at night 4-8 days after grafting, increasing the ventilation time to 3 hours in the morning and at night 9-12 days after grafting, and normally managing after 13 days.

Wherein, before step (1), cucumber seedlings are subjected to moisture control by maintaining the soil humidity at 60%; and normal water management is carried out on the pumpkin seedlings.

Example 4

Cucumber was grafted according to the method of example 1, except that, before step (1), the cucumber seedlings were subjected to moisture control by maintaining the soil humidity at 40%; and normal water management is carried out on the pumpkin seedlings.

Example 5

Cucumber was grafted according to the method of example 1, except that the cucumber was soaked in a mixed solution containing 100mg/LIAA, 10mg/L6-BA and 20mg/LALA for 15min, wherein the solvent of the mixed solution was distilled water.

Example 6

Cucumber was grafted according to the method of example 1, except that spraying the trace element foliar fertilizer solution prepared in preparation example 1 to the surface of the cucumber scion at the 3 rd and 5 th days after grafting was omitted once each.

Example 7

Cucumber was grafted according to the method of example 1, except that the trace-element foliar fertilizer solution obtained in preparation example 1 was replaced with the trace-element foliar fertilizer solution obtained in preparation example 2.

Comparative example 1

The splicing method specifically comprises the following steps:

selecting 'Jingxin anvil No. 5' pumpkin as a stock, and 'Zhongnong No. 26' cucumber as a scion, wherein the pumpkin is sowed 3 days earlier than the cucumber; when the cotyledon of the pumpkin is completely flattened and the true leaf is just exposed, the cotyledon of the cucumber is just flattened and the grafting is carried out when the true leaf is not exposed. The specific process is as follows:

removing the growing points and the true leaves of the pumpkin by using a blade, obliquely inserting a grafting needle between cotyledons at an angle of 30 degrees from one cotyledon to the other cotyledon, wherein the depth is about 0.6cm, cutting oblique planes at the same angle at a position of 1.5cm of a hypocotyl below a scion cotyledon on the basis that the grafting needle is not exposed in the cotyledon at the other side, immediately inserting the grafting needle into the scion after pulling the grafting needle out of the stock, and fixing the grafting needle by using a grafting clip. Post-grafting management was as in example 1.

Comparative example 2

Cucumber was grafted according to the method of example 1, except that when the cotyledon of the cucumber seedling started to flatten and the true leaf was not exposed (5 days after sowing), the cotyledon of the pumpkin seedling just started to flatten and the true leaf was not exposed (7 days after sowing).

Comparative example 3

Cucumber was grafted as in example 1, except that the initial cucumber scions were omitted from being soaked in the mixed solution containing 100mg/LIAA, 10mg/L6-BA and 20 mg/LALA.

Comparative example 4

Cucumber was grafted as in example 1, except that the initial cucumber scions were soaked in a mixed solution containing 100mg/LIAA and 10 mg/L6-BA.

Comparative example 5

Cucumbers were grafted according to the method of example 1, except that the initial cucumber scion after soaking was obliquely cut at 20 ° downward at 1cm below its growing point in the direction perpendicular to the cotyledon's growing direction and obliquely cut at 20 ° upward at 1cm below the growing point of the pumpkin seedling in the direction parallel to the cotyledon's growing direction.

Comparative example 6

Cucumbers were grafted according to the method of example 1, except that the initial cucumber scion after soaking was obliquely cut at 46 ° downward at 1cm below its growing point in the direction perpendicular to the cotyledon's growing direction, and obliquely cut at 46 ° upward at 1cm below the growing point of the pumpkin seedling in the direction parallel to the cotyledon's growing direction.

Test example

1. The healing time of the grafted wounds of examples 1-7 and comparative examples 1-6 was observed and recorded. And (3) determining the grafting healing time to be about 6-8d by anatomically observing the cross section of the grafting opening of 1d, 2d, 3d, 4d, 5d, 6d, 7d, 8d, 9d, 10d and 12d after grafting, and simultaneously determining the grafting of the long stem section with 6cm of the grafting seedling joint (3 cm respectively above and below) by using a digital display type push-pull force meter HF-30, wherein the approximate range of the binding force of 4-12d after grafting is 1.25-15.78N, and the binding force during grafting healing is determined to be 8.00 +/-0.5N, so as to determine the accurate grafting healing time based on the standard. 7 strains per treatment, 3 biological replicates, and the results are given in table 1 below;

2. measuring and calculating the strong seedling index of the grafted seedling of the 30 th time after grafting in the examples 1-7 and the comparative examples 1-6, wherein the strong seedling index calculation formula is as follows: the strong seedling index (stem thickness/plant height) x the total stem mass, and the results are shown in table 1 below;

3. observing and calculating the survival rate of the grafted seedlings of the 30 th day after grafting in the examples 1-7 and the comparative examples 1-6, wherein the calculation formula of the survival rate is as follows: the survival rate of the grafted seedlings is the total number of the survived grafted seedlings/grafted seedlings which survive for 30 days after grafting, and the results are shown in the following table 1;

4. the grafting aging of examples 1-7 and comparative examples 1-6 was recorded and the results are shown in table 1 below.

TABLE 1

According to the data in the table 1, the healing time of the method can be shortened to 6.22-6.83d, the efficiency during grafting reaches 91-100 plants/h, the seedling strengthening index reaches 0.78-0.92, and the survival rate reaches 92-98%. Compared with the comparative example 1, the method directly removes all the stock growing points and cotyledons, so that the problem of later-stage manual removal of residual growing points is solved, time and labor are saved, large-scale mechanical production can be carried out, excessive growth of the hypocotyl of the stock is avoided, the operation is simple, and the grafting timeliness is improved.

The preferred embodiments of the present invention have been described above in detail, but the present invention is not limited thereto. Within the scope of the technical idea of the invention, numerous simple modifications can be made to the technical solution of the invention, including combinations of the specific features in any suitable way, and the invention will not be further described in relation to the various possible combinations in order to avoid unnecessary repetition. Such simple modifications and combinations should be considered within the scope of the present disclosure as well.

Claims

1. A cucumber grafting method is characterized by comprising the following steps:

(5) respectively spraying trace element foliar fertilizer solution on the surfaces of the cucumber scions once in the 3 rd and 5 th days after grafting; based on the total weight of the trace element foliar fertilizer solution, the trace element foliar fertilizer solution contains 35-40% of choline chloride, 5-6% of amine fresh grease, 10-12% of zinc sulfate heptahydrate, 15-18% of cellulose, 0.01-0.05% of brassinolide and the balance of neutral oxidation potential water.

2. The cucumber grafting method according to claim 1, wherein the method further comprises: before the step (1), performing moisture control on cucumber seedlings by maintaining the soil humidity at 50-60%; and normal water management is carried out on the pumpkin seedlings.

3. The cucumber grafting method as claimed in claim 1, wherein the solvent of the mixed solution containing 100-110mg/LIAA, 10-12mg/L6-BA and 20-25mg/LALA is neutral oxidation potential water.

4. The cucumber grafting method as claimed in any one of claims 1-2, wherein the oxidation-reduction potential of the neutral oxidation potential water is between 800-1000, the free chlorine content is 250-950mg/L, and the pH value is 6.7-7.3.

5. Cucumber grafting method according to any of the claims 1-2, wherein the method further comprises a post-grafting temperature management comprising: the day temperature is 28-30 ℃/night temperature is 18-20 ℃.

6. Cucumber grafting method according to any of the claims 1-2, wherein the method further comprises post-grafting light management comprising: keeping out of the sun 1-3d after grafting, and then using light intensity of 100-^-2s^-1The weak light irradiates 3-5d, and the 6 th-9 th day enters normal illumination management.

7. Cucumber grafting method according to any of the claims 1-2, wherein the method further comprises post-grafting moisture management comprising: after grafting, the humidity is maintained at 80-95% in 7d, and the humidity is reduced to 60% in 8-10 d.

8. Cucumber grafting method according to any of the claims 1-2, wherein the method further comprises a post-grafting ventilation management comprising: ventilating for 2 hours in the morning and at night 4-8 days after grafting, increasing the ventilation time to 3 hours in the morning and at night 9-12 days after grafting, and normally managing after 13 days.