CN108739243A - A method of promoting dendrobe tissue culture seedling planting survival rates - Google Patents

A method of promoting dendrobe tissue culture seedling planting survival rates Download PDF

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Publication number
CN108739243A
CN108739243A CN201810450522.XA CN201810450522A CN108739243A CN 108739243 A CN108739243 A CN 108739243A CN 201810450522 A CN201810450522 A CN 201810450522A CN 108739243 A CN108739243 A CN 108739243A
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parts
tissue culture
moso bamboo
culture seedling
planting
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檀凤珍
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Anhui Huoshan Caozhiling Traditional Chinese Medicine Materials Co Ltd
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Anhui Huoshan Caozhiling Traditional Chinese Medicine Materials Co Ltd
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Priority to CN201810450522.XA priority Critical patent/CN108739243A/en
Publication of CN108739243A publication Critical patent/CN108739243A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15Calcined rock, e.g. perlite, vermiculite or clay aggregates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers

Abstract

The present invention discloses a kind of method promoting dendrobe tissue culture seedling planting survival rates, including following operating procedure:(1)After dried silkworm chrysalis meal, red worm powder, honeycomb powder, grape seed oil are mixed, continue that 23-26 parts of water are added thereto, after being uniformly mixed, is added into pelleter, organic matter particle is made;(2)After mixing by glass microballoon, vermiculite, biogas residue, peat soil, organic matter particle, water is added thereto, continues after stirring evenly, mixture is put into closed container, cultivation matrix is made in ripening;(3)Cultivation matrix is filled into moso bamboo Planting utensil, temporary planting the previous day sprinkles profoundly water, for use;(4)Dendrobe tissue culture seedling after hardening is planted in moso bamboo Planting utensil, then sprays a liquid fertilizer weekly.The method provided by the invention for promoting dendrobe tissue culture seedling planting survival rates can effectively promote the survival rate after the field planting of dendrobe tissue culture seedling, reduce the loss of farmer, promote the yield of the stem of noble dendrobium.

Description

A method of promoting dendrobe tissue culture seedling planting survival rates
Technical field
The invention belongs to dendrobe cultivation technical fields, and in particular to a kind of side promoting dendrobe tissue culture seedling planting survival rates Method.
Background technology
Stem of noble dendrobium happiness is grown in warm, moist, half cloudy half light environment, wet with 1000 millimeters of annual rainfall or more, air Degree, which is more than to grow in subtropical zone remote, thickly forested mountains of 80%, 1 monthly mean temperature higher than 8 DEG C, to be preferred, and not very stringent, open country is required clay fertilizer Life is mostly grown on loose and thick bark or trunk, and some is also grown in crack of stone.But the stem of noble dendrobium is under natural environmental condition Self-reproduction rate is very low, and the growth and development of the stem of noble dendrobium is slow, so the group culturation rapid propagating technology of the stem of noble dendrobium has obtained rapid development, but Since tissue culture environment and artificial cultivation environment are there are larger difference, tissue-cultured seedling needs are adapted to the external world by gnotobasis certainly comprehensively Right environment is transitioned into autotrophy by heterotrophism, and the transplanting survival rate of tissue-cultured seedling is low, and especially the stem of noble dendrobium carries out survival rate when Emulational culture It is lower, such as directly survival rate of the cultivation to Emulational culture base is about 30% or so after Dendrobidium huoshanness tissue-cultured seedling bottle outlet.
Invention content
In order to solve the above technical problem, the present invention provides a kind of dendrobe tissue culture seedling planting survival rates of being obviously improved Method.
The present invention is achieved by the following technical solutions.
A method of promoting dendrobe tissue culture seedling planting survival rates, including following operating procedure:
(1)By weight, 32-39 parts of dried silkworm chrysalis meals, 11-16 parts of red worm powder, 20-25 parts of honeycomb powder, 4-6 parts of grape seed oils are mixed After conjunction, continue that 23-26 parts of water are added thereto, after being uniformly mixed, be added into pelleter, obtained average grain diameter is The organic matter particle of 0.8-1.2mm;
(2)By weight, by 45-50 parts of glass microballoons, 11-14 parts of vermiculites, 16-20 parts of biogas residues, 14-19 parts of peat soils, 7- 10 parts of organic matter particles after mixing, are added 30-35 parts of water, continue after stirring evenly, mixture is put into closed thereto In container, cultivation matrix is made in ripening 3-4 days;
(3)The moso bamboo for selecting a diameter of 8-12cm, it is sawed-off at moso bamboo section, moso bamboo Planting utensil is made, by moso bamboo Planting utensil upper end Opening polishes, and the depth of control moso bamboo Planting utensil is 7-9cm, and the bottom of moso bamboo Planting utensil is opened to the circle of 3-5 a diameter of 1cm Then cultivation matrix is filled in hole into moso bamboo Planting utensil, struck off the cultivation matrix on moso bamboo Planting utensil surface with small plank, temporary planting The previous day sprinkles profoundly water, for use;
(4)By the dendrobe tissue culture seedling after hardening with 4-6 single plant for one clump, each moso bamboo Planting utensil is planted one clump, then weekly A liquid fertilizer is sprayed, wherein liquid fertilizer is made of the component of following parts by weight:1-3 parts of courage phosphorus choline, ethylenediamine base propanesulfonate 4-8 Part, 24-29 parts of potassium dihydrogen phosphate, 15-19 parts of urea, 400-500 parts of water.
Specifically, above-mentioned steps(1)In, honeycomb powder is made of following methods:Honeycomb is dried, average grain diameter is crushed to After 60-80 mesh, honeycomb powder is made.
Specifically, above-mentioned steps(2)In, the Average Particle Diameters of glass microballoon are 2-3mm, the Average Particle Diameters of vermiculite For 0.4-0.6mm.
Specifically, above-mentioned steps(2)In, in biogas residue containing mass fraction be 50% organic matter, mass fraction be 20% The full potassium that full phosphorus that full nitrogen that humic acid, mass fraction are 2%, mass fraction are 1.2%, mass fraction are 1.8%, surplus is water.
Specifically, above-mentioned steps(4)In, every clump of dendrobe tissue culture seedling sprinkling liquid fertilizer 80-100g.
From the above technical scheme, it can be seen that the beneficial effects of the invention are as follows:
The method provided by the invention for promoting dendrobe tissue culture seedling planting survival rates, easy to operate, cost is relatively low, and scale is suitble to plant It trains the stem of noble dendrobium to use, can effectively promote the survival rate after the field planting of dendrobe tissue culture seedling, reduce the loss of farmer, promote the production of the stem of noble dendrobium It measures, growth of seedling stalwartness, neat and consistent in moso bamboo Planting utensil, and the good characteristic for also having slow seedling fast after being colonized.Wherein, Step(1)Organic principle is absorbed containing the abundant dendrobe tissue culture seedling that is conducive in organic matter particle obtained, dried silkworm chrysalis meal and red worm powder, Energy expenditure when dendrobe tissue culture seedling absorbs nutriment can be reduced;In honeycomb powder, containing abundant propolis, honeycomb oil component, It has the function of softening tissue-cultured seedling root okioplast, the gas permeability of promotion tissue-cultured seedling root cell and the suction to nutriment Receipts ability, and then adaptability of the tissue-cultured seedling to environment effectively is improved, improve the survival rate after its field planting;Step(4)In Liquid fertilizer, courage phosphorus choline can effectively promote the activity of various enzymes in tissue-cultured seedling, enhance the efficiency that nutrient synthesizes in tissue-cultured seedling, promote Into the development of tissue-cultured seedling, and then improve the survival rate after its transplanting;After the present invention selects moso bamboo to be planted as tissue-cultured seedling Planting utensil, influence degree of the humiture to moso bamboo in environment is close with the influence degree to cultivation matrix, and then can be effective Promote adaptive faculty of the tissue-cultured seedling to environment.
Specific implementation mode
Following embodiment cannot be used for limiting the scope of the invention for illustrating the present invention.The reality used in embodiment The condition of applying can be for further adjustments according to the condition of producer, and unaccounted implementation condition is usually conventional laboratory conditions.
Embodiment 1
A method of promoting dendrobe tissue culture seedling planting survival rates, including following operating procedure:
(1)By weight, will after 32 parts of dried silkworm chrysalis meals, 11 parts of red worm powder, 20 parts of honeycomb powder, 4 parts of grape seed oils mix, continue to 23 parts of water are wherein added, after being uniformly mixed, are added into pelleter, the organic matter that average grain diameter is 0.8mm is made Particle;
(2)By weight, 45 parts of glass microballoons, 11 parts of vermiculites, 16 parts of biogas residues, 14 parts of peat soils, 7 parts of organic matter particles are mixed After closing uniformly, 30 parts of water are added thereto, continues after stirring evenly, mixture is put into closed container, ripening 3 days, Cultivation matrix is made;
(3)The moso bamboo for selecting a diameter of 8cm, it is sawed-off at moso bamboo section, moso bamboo Planting utensil is made, by moso bamboo Planting utensil upper end opening Place polishes, and the depth of control moso bamboo Planting utensil is 7cm, the bottom of moso bamboo Planting utensil is opened to the circular hole of 3 a diameter of 1cm, then Cultivation matrix is filled into moso bamboo Planting utensil, is struck off the cultivation matrix on moso bamboo Planting utensil surface with small plank, is planted the previous day It sprinkles profoundly water, for use;
(4)By the dendrobe tissue culture seedling after hardening with 4-6 single plant for one clump, each moso bamboo Planting utensil is planted one clump, then weekly A liquid fertilizer is sprayed, wherein liquid fertilizer is made of the component of following parts by weight:1 part of courage phosphorus choline, 4 parts of ethylenediamine base propanesulfonate, 24 parts of potassium dihydrogen phosphate, 15 parts of urea, 400 parts of water.
Specifically, above-mentioned steps(1)In, honeycomb powder is made of following methods:Honeycomb is dried, average grain diameter is crushed to After 60 mesh, honeycomb powder is made.
Specifically, above-mentioned steps(2)In, the Average Particle Diameters of glass microballoon are 2mm, and the Average Particle Diameters of vermiculite are 0.4mm。
Specifically, above-mentioned steps(2)In, in biogas residue containing mass fraction be 50% organic matter, mass fraction be 20% The full potassium that full phosphorus that full nitrogen that humic acid, mass fraction are 2%, mass fraction are 1.2%, mass fraction are 1.8%, surplus is water.
Specifically, above-mentioned steps(4)In, every clump of dendrobe tissue culture seedling sprinkling liquid fertilizer 80g.
Embodiment 2
A method of promoting dendrobe tissue culture seedling planting survival rates, including following operating procedure:
(1)By weight, will after 34 parts of dried silkworm chrysalis meals, 13 parts of red worm powder, 23 parts of honeycomb powder, 5 parts of grape seed oils mix, continue to 25 parts of water are wherein added, after being uniformly mixed, are added into pelleter, the organic matter that average grain diameter is 1.0mm is made Particle;
(2)By weight, 48 parts of glass microballoons, 13 parts of vermiculites, 18 parts of biogas residues, 17 parts of peat soils, 8 parts of organic matter particles are mixed After closing uniformly, 33 parts of water are added thereto, continues after stirring evenly, mixture is put into closed container, ripening 4 days, Cultivation matrix is made;
(3)The moso bamboo for selecting a diameter of 10cm, it is sawed-off at moso bamboo section, moso bamboo Planting utensil is made, moso bamboo Planting utensil upper end is opened It is polished at mouthful, the depth of control moso bamboo Planting utensil is 8cm, the bottom of moso bamboo Planting utensil is opened to the circular hole of 4 a diameter of 1cm, so Cultivation matrix is filled in backward moso bamboo Planting utensil, is struck off the cultivation matrix on moso bamboo Planting utensil surface with small plank, temporary planting is previous It is sprinkled profoundly water, for use;
(4)By the dendrobe tissue culture seedling after hardening with 4-6 single plant for one clump, each moso bamboo Planting utensil is planted one clump, then weekly A liquid fertilizer is sprayed, wherein liquid fertilizer is made of the component of following parts by weight:3 parts of courage phosphorus choline, 6 parts of ethylenediamine base propanesulfonate, 27 parts of potassium dihydrogen phosphate, 17 parts of urea, 450 parts of water.
Specifically, above-mentioned steps(1)In, honeycomb powder is made of following methods:Honeycomb is dried, average grain diameter is crushed to After 70 mesh, honeycomb powder is made.
Specifically, above-mentioned steps(2)In, the Average Particle Diameters of glass microballoon are 2mm, and the Average Particle Diameters of vermiculite are 0.5mm。
Specifically, above-mentioned steps(2)In, in biogas residue containing mass fraction be 50% organic matter, mass fraction be 20% The full potassium that full phosphorus that full nitrogen that humic acid, mass fraction are 2%, mass fraction are 1.2%, mass fraction are 1.8%, surplus is water.
Specifically, above-mentioned steps(4)In, every clump of dendrobe tissue culture seedling sprinkling liquid fertilizer 90g.
Embodiment 3
A method of promoting dendrobe tissue culture seedling planting survival rates, including following operating procedure:
(1)By weight, will after 39 parts of dried silkworm chrysalis meals, 16 parts of red worm powder, 25 parts of honeycomb powder, 6 parts of grape seed oils mix, continue to 26 parts of water are wherein added, after being uniformly mixed, are added into pelleter, the organic matter that average grain diameter is 1.2mm is made Particle;
(2)By weight, by 50 parts of glass microballoons, 14 parts of vermiculites, 20 parts of biogas residues, 19 parts of peat soils, 10 parts of organic matter particles After mixing, 35 parts of water are added thereto, continues after stirring evenly, mixture is put into closed container, ripening 4 It, is made cultivation matrix;
(3)The moso bamboo for selecting a diameter of 12cm, it is sawed-off at moso bamboo section, moso bamboo Planting utensil is made, moso bamboo Planting utensil upper end is opened It is polished at mouthful, the depth of control moso bamboo Planting utensil is 9cm, the bottom of moso bamboo Planting utensil is opened to the circular hole of 5 a diameter of 1cm, so Cultivation matrix is filled in backward moso bamboo Planting utensil, is struck off the cultivation matrix on moso bamboo Planting utensil surface with small plank, temporary planting is previous It is sprinkled profoundly water, for use;
(4)By the dendrobe tissue culture seedling after hardening with 4-6 single plant for one clump, each moso bamboo Planting utensil is planted one clump, then weekly A liquid fertilizer is sprayed, wherein liquid fertilizer is made of the component of following parts by weight:3 parts of courage phosphorus choline, 8 parts of ethylenediamine base propanesulfonate, 29 parts of potassium dihydrogen phosphate, 19 parts of urea, 500 parts of water.
Specifically, above-mentioned steps(1)In, honeycomb powder is made of following methods:Honeycomb is dried, average grain diameter is crushed to After 80 mesh, honeycomb powder is made.
Specifically, above-mentioned steps(2)In, the Average Particle Diameters of glass microballoon are 3mm, and the Average Particle Diameters of vermiculite are 0.6mm。
Specifically, above-mentioned steps(2)In, in biogas residue containing mass fraction be 50% organic matter, mass fraction be 20% The full potassium that full phosphorus that full nitrogen that humic acid, mass fraction are 2%, mass fraction are 1.2%, mass fraction are 1.8%, surplus is water.
Specifically, above-mentioned steps(4)In, every clump of dendrobe tissue culture seedling sprinkling liquid fertilizer 100g.
Comparative example 1
Step(1)In, by weight, after 32 parts of soy meals, 16 parts of fish meal, 1-3 parts of grape seed oils are mixed, continue thereto 20 parts of water are added, after being uniformly mixed, are added into pelleter, the organic matter particle that average grain diameter is 0.8mm is made; Remaining operating procedure is identical with embodiment 1.
Comparative example 2
Step(4)In liquid fertilizer be clear water, remaining operating procedure is identical with embodiment 2.
Temporary planting experiment is carried out to same a collection of Dendrobidium huoshanness tissue-cultured seedling with the method for each embodiment and comparative example respectively, experiment Number of days is to carry out Transplanting Test to temporary planting hardening after 5 months, 5 months, and test result is as shown in table 1:
A kind of compliance test result promoting dendrobe tissue culture seedling planting survival rates method of table 1
As shown in Table 1, organic matter particle produced by the present invention is easy to be absorbed by Dendrobidium huoshanness, can effectively promote Dendrobidium huoshanness The survival rate and the survival rate after transplanting of tissue-cultured seedling, while liquid fertilizer provided by the invention also can greatly promote depositing for Dendrobidium huoshanness Motility rate ensure that the yield of Dendrobidium huoshanness.
It should be noted that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting.Although with reference to compared with Good embodiment describes the invention in detail, it will be understood by those of ordinary skill in the art that, it can be to the technology of invention Scheme is modified or replaced equivalently, and without departing from the range of technical solution of the present invention, should all cover the power in the present invention In sharp claimed range.

Claims (5)

1. a kind of method promoting dendrobe tissue culture seedling planting survival rates, which is characterized in that including following operating procedure:
(1)By weight, 32-39 parts of dried silkworm chrysalis meals, 11-16 parts of red worm powder, 20-25 parts of honeycomb powder, 4-6 parts of grape seed oils are mixed After conjunction, continue that 23-26 parts of water are added thereto, after being uniformly mixed, be added into pelleter, obtained average grain diameter is The organic matter particle of 0.8-1.2mm;
(2)By weight, by 45-50 parts of glass microballoons, 11-14 parts of vermiculites, 16-20 parts of biogas residues, 14-19 parts of peat soils, 7- 10 parts of organic matter particles after mixing, are added 30-35 parts of water, continue after stirring evenly, mixture is put into closed thereto In container, cultivation matrix is made in ripening 3-4 days;
(3)The moso bamboo for selecting a diameter of 8-12cm, it is sawed-off at moso bamboo section, moso bamboo Planting utensil is made, by moso bamboo Planting utensil upper end Opening polishes, and the depth of control moso bamboo Planting utensil is 7-9cm, and the bottom of moso bamboo Planting utensil is opened to the circle of 3-5 a diameter of 1cm Then cultivation matrix is filled in hole into moso bamboo Planting utensil, struck off the cultivation matrix on moso bamboo Planting utensil surface with small plank, temporary planting The previous day sprinkles profoundly water, for use;
(4)By the dendrobe tissue culture seedling after hardening with 4-6 single plant for one clump, each moso bamboo Planting utensil is planted one clump, then weekly A liquid fertilizer is sprayed, wherein liquid fertilizer is made of the component of following parts by weight:1-3 parts of courage phosphorus choline, ethylenediamine base propanesulfonate 4-8 Part, 24-29 parts of potassium dihydrogen phosphate, 15-19 parts of urea, 400-500 parts of water.
2. a kind of method promoting dendrobe tissue culture seedling planting survival rates according to claim 1, which is characterized in that above-mentioned step Suddenly(1)In, honeycomb powder is made of following methods:Honeycomb is dried, it is obtained honeycomb after 60-80 mesh to be crushed to average grain diameter Powder.
3. a kind of method promoting dendrobe tissue culture seedling planting survival rates according to claim 1, which is characterized in that above-mentioned step Suddenly(2)In, the Average Particle Diameters of glass microballoon are 2-3mm, and the Average Particle Diameters of vermiculite are 0.4-0.6mm.
4. a kind of method promoting dendrobe tissue culture seedling planting survival rates according to claim 1, which is characterized in that above-mentioned step Suddenly(2)In, in biogas residue containing mass fraction be 50% organic matter, mass fraction be 20% humic acid, mass fraction be 2% The full potassium that full phosphorus that full nitrogen, mass fraction are 1.2%, mass fraction are 1.8%, surplus is water.
5. a kind of method promoting dendrobe tissue culture seedling planting survival rates according to claim 1, which is characterized in that above-mentioned step Suddenly(4)In, every clump of dendrobe tissue culture seedling sprinkling liquid fertilizer 80-100g.
CN201810450522.XA 2018-05-11 2018-05-11 A method of promoting dendrobe tissue culture seedling planting survival rates Pending CN108739243A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104987207A (en) * 2015-06-30 2015-10-21 柳州市天姿园艺有限公司 Dendrobium nobile tissue culture substrate
CN105248265A (en) * 2015-11-04 2016-01-20 富阳市文曲生态农业开发有限公司 Method of planting dendrobium officinale by using moso bamboo
CN105918084A (en) * 2016-04-25 2016-09-07 广西智宝科技有限公司 Wild-imitating planting substrate for dendrobium huoshanense and preparation method for wild-imitating planting substrate
CN105918085A (en) * 2016-04-27 2016-09-07 覃伟 Dendrobium huoshanense special-purpose planting matrix and preparation method thereof
CN105948839A (en) * 2016-02-29 2016-09-21 浙江省农业科学院 Preparation and application method of organic fertilizer specially used for dendrobium officinale
CN206024634U (en) * 2016-08-31 2017-03-22 福建永耕农业开发有限公司 Portable stem of noble dendrobium formation stores pylon

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104987207A (en) * 2015-06-30 2015-10-21 柳州市天姿园艺有限公司 Dendrobium nobile tissue culture substrate
CN105248265A (en) * 2015-11-04 2016-01-20 富阳市文曲生态农业开发有限公司 Method of planting dendrobium officinale by using moso bamboo
CN105948839A (en) * 2016-02-29 2016-09-21 浙江省农业科学院 Preparation and application method of organic fertilizer specially used for dendrobium officinale
CN105918084A (en) * 2016-04-25 2016-09-07 广西智宝科技有限公司 Wild-imitating planting substrate for dendrobium huoshanense and preparation method for wild-imitating planting substrate
CN105918085A (en) * 2016-04-27 2016-09-07 覃伟 Dendrobium huoshanense special-purpose planting matrix and preparation method thereof
CN206024634U (en) * 2016-08-31 2017-03-22 福建永耕农业开发有限公司 Portable stem of noble dendrobium formation stores pylon

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