CN108738477A - A kind of production method for exempting from broken shell aspermous watermelon seed - Google Patents
A kind of production method for exempting from broken shell aspermous watermelon seed Download PDFInfo
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- CN108738477A CN108738477A CN201810616962.8A CN201810616962A CN108738477A CN 108738477 A CN108738477 A CN 108738477A CN 201810616962 A CN201810616962 A CN 201810616962A CN 108738477 A CN108738477 A CN 108738477A
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- aspermous watermelon
- watermelon seed
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- broken shell
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of production methods for exempting from broken shell aspermous watermelon seed, include the following steps:The selection of S1, aspermous watermelon seed;The first time disinfection of S2, aspermous watermelon seed;Second of disinfection of S3, aspermous watermelon seed;The third time disinfection of S4, aspermous watermelon seed;S5, artificial broken shell is carried out to aspermous watermelon seed;S6, the aspermous watermelon seed after broken shell is immersed in Gibberellins solution, and dries to obtain in room temperature and exempts from broken shell aspermous watermelon seed, broken shell aspermous watermelon seed will be exempted from and be vacuum-packed;The present invention, pass through the disinfection three times to aspermous watermelon seed, the bacterium of aspermous watermelon the surface of the seed and virus are thoroughly eliminated, it is influenced by bacterium and virus to reduce aspermous watermelon seed, the survival rate for improving seed, exempting from broken shell aspermous watermelon seed can directly sow, and bring great convenience to grower, and germination percentage is high, reduces the production cost of client.
Description
Technical field
The present invention relates to seed breeding technique field more particularly to a kind of production methods for exempting from broken shell aspermous watermelon seed.
Background technology
Existing seed is all common aspermous watermelon seed, and grower needs voluntarily after buying after aspermous watermelon seed
Broken shell is carried out to watermelon seed, the planting percent of growth of watermelon can be reduced, and of high cost, the bad problem of watermelon seedling can be caused,
Improve the production cost of grower.It is typically all directly broken to seed in the existing method to aspermous watermelon seed broken shell
Shell causes seed to be easy to be infected by bacterium or virus, reduces the germination percentage of watermelon seed.
Therefore, we have proposed a kind of production methods for exempting from broken shell aspermous watermelon seed for solving the above problems.
Invention content
The purpose of the present invention is to solve aspermous watermelon seed exists in the prior art to need grower's voluntarily broken shell, lead
The disadvantage for causing the germination percentage of aspermous watermelon seed low, and a kind of production method that exempting from broken shell aspermous watermelon seed proposed.
A kind of production method for exempting from broken shell aspermous watermelon seed, includes the following steps:
The selection of S1, aspermous watermelon seed:Select that grain is full greatly, the good watermelon seed of regularity, it is small to reject grain, lopsided and not plump shape
The watermelon seed of son;
The first time disinfection of S2, aspermous watermelon seed:It, will be without seed after the seed of aspermous watermelon is impregnated 3h-4h in tap water
Watermelon seed takes out and is placed in disinfectant and takes out aspermous watermelon seed after immersion 3h-4h, and using distilled water to nothing
Seed watermelon seed is rinsed 20min-30min;
Second of disinfection of S3, aspermous watermelon seed:The seed of aspermous watermelon is immersed in the distilled water that temperature is 50 DEG C -60 DEG C
Middle 30min, and the temperature-resistant of distilled water is kept, and the seed of aspermous watermelon is constantly stirred, by aspermous watermelon seed
Distilled water flushing 2h-3h is used after taking-up;
The third time disinfection of S4, aspermous watermelon seed:The seed of aspermous watermelon is immersed in the distilled water that temperature is 75 DEG C
30min is constantly stirred the seed of aspermous watermelon, and the temperature of distilled water is made to be gradually decrease to 50 DEG C -60 DEG C, by nothing
The seed of seed watermelon will be spare after the seed taking-up drying of aspermous watermelon after impregnating 4h-6h;
S5, artificial broken shell is carried out to aspermous watermelon seed;
S6, the aspermous watermelon seed after broken shell is immersed in Gibberellins solution, and dries to obtain in room temperature and exempts from broken shell without seed west
Melon seed will be exempted from broken shell aspermous watermelon seed and is vacuum-packed.
Preferably, it is female parent, diploid watermelon seed that the source of the aspermous watermelon seed, which is with tetraploid watermelon seed,
For male parent, hybridization obtains aspermous watermelon seed.
Preferably, the disinfectant is copper-bath, one kind in liquor potassic permanganate and trisodium phosphate solution, and
The mass fraction of copper-bath is 0.3%-1%, and the mass fraction of liquor potassic permanganate is 0.4%-0.8%, trisodium phosphate solution
Mass fraction be 1%-2%.
Preferably, a concentration of 50mg/l-150mg/l of the Gibberellins solution, and aspermous watermelon seed is molten in gibberellin
Soaking time in liquid is 12h-36h.
Preferably, the method for the artificial broken shell is gently to push the tip of aspermous watermelon seed aside using nipper pliers, is made
Aspermous watermelon seed umbilical region suture splits, and the length that suture splits is the 1/3 of seed length.
Preferably, the production for exempting from broken shell aspermous watermelon seed carries out in the workshops GMP.
The beneficial effects of the invention are as follows:
1, of the invention, pass through the disinfection three times to aspermous watermelon seed so that the bacterium of aspermous watermelon the surface of the seed and virus can
Thoroughly to be eliminated, is influenced by bacterium and virus to reduce aspermous watermelon seed, improve the survival rate of seed.
2, of the invention, the seed of aspermous watermelon is impregnated by using gibberellin, can effectively improve aspermous watermelon kind
The germination percentage of son, improves the economic benefit of farmer.
3, of the invention, broken shell aspermous watermelon seed produced by the invention of exempting from can be sowed directly, and pole is brought to grower
Big convenience, and exempt from broken shell aspermous watermelon percentage of seedgermination height, greatly reduce the production cost of client.
Specific implementation mode
The present invention is made further to explain with reference to specific embodiment.
Embodiment one
A kind of production method for exempting from broken shell aspermous watermelon seed, includes the following steps:
S1, aspermous watermelon seed(It is female parent, diploid watermelon seed that the source of aspermous watermelon seed, which is with tetraploid watermelon seed,
For male parent, hybridization obtains aspermous watermelon seed)Selection:Select that grain is full greatly, the good watermelon seed of regularity, it is small to reject grain,
The watermelon seed of lopsided and not plump shape;
The first time disinfection of S2, aspermous watermelon seed:After the seed of aspermous watermelon is impregnated 3h in tap water, by aspermous watermelon
Seed is taken out and is placed in the copper-bath that mass fraction is 0.6 and takes out aspermous watermelon seed after immersion 3h, and is used
Distilled water is rinsed 30min to aspermous watermelon seed;
Second of disinfection of S3, aspermous watermelon seed:The seed of aspermous watermelon is immersed in the distilled water that temperature is 50 DEG C
30min, and the temperature-resistant of distilled water is kept, and the seed of aspermous watermelon is constantly stirred, aspermous watermelon seed is taken
Distilled water flushing 3h is used after going out;
The third time disinfection of S4, aspermous watermelon seed:The seed of aspermous watermelon is immersed in the distilled water that temperature is 75 DEG C
30min is constantly stirred the seed of aspermous watermelon, and the temperature of distilled water is made to be gradually decrease to 50 DEG C, will be without seed west
The seed of melon will be spare after the seed taking-up drying of aspermous watermelon after impregnating 4h;
S5, artificial broken shell is carried out to aspermous watermelon seed:The tip for gently pushing aspermous watermelon seed aside using nipper pliers, makes nothing
Seed watermelon seed umbilical region suture splits, and the length that suture splits is the 1/3 of seed length;
S6, the aspermous watermelon seed after broken shell is immersed in the Gibberellins solution of a concentration of 50mg/l for 24 hours, and dried in the air in room temperature
It is dry to obtain exempting from broken shell aspermous watermelon seed, broken shell aspermous watermelon seed will be exempted from and be vacuum-packed, broken shell aspermous watermelon seed is exempted from
Production carried out in the workshops GMP.
Embodiment two
A kind of production method for exempting from broken shell aspermous watermelon seed, includes the following steps:
S1, aspermous watermelon seed(It is female parent, diploid watermelon seed that the source of aspermous watermelon seed, which is with tetraploid watermelon seed,
For male parent, hybridization obtains aspermous watermelon seed)Selection:Select that grain is full greatly, the good watermelon seed of regularity, it is small to reject grain,
The watermelon seed of lopsided and not plump shape;
The first time disinfection of S2, aspermous watermelon seed:After the seed of aspermous watermelon is impregnated 4h in tap water, by aspermous watermelon
Seed is taken out and is placed in the liquor potassic permanganate that mass fraction is 0.6% and takes out aspermous watermelon seed after immersion 4h, and is made
30min is rinsed to aspermous watermelon seed with distilled water;
Second of disinfection of S3, aspermous watermelon seed:The seed of aspermous watermelon is immersed in the distilled water that temperature is 55 DEG C
30min, and the temperature-resistant of distilled water is kept, and the seed of aspermous watermelon is constantly stirred, aspermous watermelon seed is taken
Distilled water flushing 3h is used after going out;
The third time disinfection of S4, aspermous watermelon seed:The seed of aspermous watermelon is immersed in the distilled water that temperature is 75 DEG C
30min is constantly stirred the seed of aspermous watermelon, and the temperature of distilled water is made to be gradually decrease to 55 DEG C, will be without seed west
The seed of melon will be spare after the seed taking-up drying of aspermous watermelon after impregnating 6h;
S5, artificial broken shell is carried out to aspermous watermelon seed:The tip for gently pushing aspermous watermelon seed aside using nipper pliers, makes nothing
Seed watermelon seed umbilical region suture splits, and the length that suture splits is the 1/3 of seed length;
S6, the aspermous watermelon seed after broken shell is immersed in the Gibberellins solution of a concentration of 100mg/l for 24 hours, and in room temperature
It dries to obtain and exempts from broken shell aspermous watermelon seed, broken shell aspermous watermelon seed will be exempted from and be vacuum-packed, broken shell aspermous watermelon kind is exempted from
The production of son carries out in the workshops GMP.
Embodiment three
A kind of production method for exempting from broken shell aspermous watermelon seed, includes the following steps:
S1, aspermous watermelon seed(It is female parent, diploid watermelon seed that the source of aspermous watermelon seed, which is with tetraploid watermelon seed,
For male parent, hybridization obtains aspermous watermelon seed)Selection:Select that grain is full greatly, the good watermelon seed of regularity, it is small to reject grain,
The watermelon seed of lopsided and not plump shape;
The first time disinfection of S2, aspermous watermelon seed:After the seed of aspermous watermelon is impregnated 4h in tap water, by aspermous watermelon
Seed is taken out and is placed in the trisodium phosphate solution that mass fraction is 1.3% and takes out aspermous watermelon seed after immersion 4h, and is made
30min is rinsed to aspermous watermelon seed with distilled water;
Second of disinfection of S3, aspermous watermelon seed:The seed of aspermous watermelon is immersed in the distilled water that temperature is 60 DEG C
30min, and the temperature-resistant of distilled water is kept, and the seed of aspermous watermelon is constantly stirred, aspermous watermelon seed is taken
Distilled water flushing 3h is used after going out;
The third time disinfection of S4, aspermous watermelon seed:The seed of aspermous watermelon is immersed in the distilled water that temperature is 75 DEG C
30min is constantly stirred the seed of aspermous watermelon, and the temperature of distilled water is made to be gradually decrease to 60 DEG C, will be without seed west
The seed of melon will be spare after the seed taking-up drying of aspermous watermelon after impregnating 6h;
S5, artificial broken shell is carried out to aspermous watermelon seed:The tip for gently pushing aspermous watermelon seed aside using nipper pliers, makes nothing
Seed watermelon seed umbilical region suture splits, and the length that suture splits is the 1/3 of seed length;
S6, the aspermous watermelon seed after broken shell is immersed in the Gibberellins solution of a concentration of 150mg/l for 24 hours, and in room temperature
It dries to obtain and exempts from broken shell aspermous watermelon seed, broken shell aspermous watermelon seed will be exempted from and be vacuum-packed, broken shell aspermous watermelon kind is exempted from
The production of son carries out in the workshops GMP.
By embodiment one, the broken shell aspermous watermelon seed of exempting from that embodiment two and embodiment three are prepared respectively takes 1000,
It will exempt from broken shell aspermous watermelon seed to be planted in sterile vegetative soil, and observe its germination percentage, the results are shown in table below:
Embodiment one | Embodiment two | Embodiment three | |
Germination percentage(%) | 96.5 | 97.3 | 96.8 |
By above-mentioned result it is found that a kind of exempting from brokenly of producing of production method for exempting from broken shell aspermous watermelon seed proposed by the present invention
The germination percentage of shell aspermous watermelon seed is high, and embodiment two is highly preferred embodiment of the present invention.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Any one skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Present inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (6)
1. a kind of production method for exempting from broken shell aspermous watermelon seed, which is characterized in that include the following steps:
The selection of S1, aspermous watermelon seed:Select that grain is full greatly, the good watermelon seed of regularity, it is small to reject grain, lopsided and not plump shape
The watermelon seed of son;
The first time disinfection of S2, aspermous watermelon seed:It, will be without seed after the seed of aspermous watermelon is impregnated 3h-4h in tap water
Watermelon seed takes out and is placed in disinfectant and takes out aspermous watermelon seed after immersion 3h-4h, and using distilled water to nothing
Seed watermelon seed is rinsed 20min-30min;
Second of disinfection of S3, aspermous watermelon seed:The seed of aspermous watermelon is immersed in the distilled water that temperature is 50 DEG C -60 DEG C
Middle 30min, and the temperature-resistant of distilled water is kept, and the seed of aspermous watermelon is constantly stirred, by aspermous watermelon seed
Distilled water flushing 2h-3h is used after taking-up;
The third time disinfection of S4, aspermous watermelon seed:The seed of aspermous watermelon is immersed in the distilled water that temperature is 75 DEG C
30min is constantly stirred the seed of aspermous watermelon, and the temperature of distilled water is made to be gradually decrease to 50 DEG C -60 DEG C, by nothing
The seed of seed watermelon will be spare after the seed taking-up drying of aspermous watermelon after impregnating 4h-6h;
S5, artificial broken shell is carried out to aspermous watermelon seed;
S6, the aspermous watermelon seed after broken shell is immersed in Gibberellins solution, and dries to obtain in room temperature and exempts from broken shell without seed west
Melon seed will be exempted from broken shell aspermous watermelon seed and is vacuum-packed.
2. a kind of production method for exempting from broken shell aspermous watermelon seed according to claim 1, which is characterized in that the no seed
It is female parent that the source of watermelon seed, which is with tetraploid watermelon seed, and diploid watermelon seed is male parent, and hybridization obtains aspermous watermelon
Seed.
3. a kind of production method for exempting from broken shell aspermous watermelon seed according to claim 1, which is characterized in that the disinfection
Liquid medicine is copper-bath, one kind in liquor potassic permanganate and trisodium phosphate solution, and the mass fraction of copper-bath is
The mass fraction of 0.3%-1%, liquor potassic permanganate are 0.4%-0.8%, and the mass fraction of trisodium phosphate solution is 1%-2%.
4. a kind of production method for exempting from broken shell aspermous watermelon seed according to claim 1, which is characterized in that described red mould
A concentration of 50mg/l-150mg/l of plain solution, and soaking time of the aspermous watermelon seed in Gibberellins solution is 12h-36h.
5. a kind of production method for exempting from broken shell aspermous watermelon seed according to claim 1, which is characterized in that described artificial
The method of broken shell is gently to push the tip of aspermous watermelon seed aside using nipper pliers, and aspermous watermelon seed umbilical region suture is made to split
It opens, and the length that suture splits is the 1/3 of seed length.
6. a kind of production method for exempting from broken shell aspermous watermelon seed according to claim 1, which is characterized in that described to exempt from brokenly
The production of shell aspermous watermelon seed carries out in the workshops GMP.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110692308A (en) * | 2019-10-14 | 2020-01-17 | 中国农业科学院郑州果树研究所 | Seedless watermelon shell-breaking-free germination accelerating method |
CN111149461A (en) * | 2020-01-21 | 2020-05-15 | 曹哲峰 | Cucurbitaceae seed shell treatment method |
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JP2010200628A (en) * | 2009-02-27 | 2010-09-16 | Nanto Seed Co Ltd | Method for sterilizing seeds and seeds |
CN103988604A (en) * | 2014-04-23 | 2014-08-20 | 黄少伟 | Seedless watermelon seed germination accelerating method |
CN104969685A (en) * | 2014-04-09 | 2015-10-14 | 芜湖美农农业科技有限公司 | Production method of directly-seeding-able shell-breaking-free seedless watermelon seeds |
CN105723848A (en) * | 2014-12-06 | 2016-07-06 | 哈尔滨弘睿翔科技开发有限公司 | Watermelon seed selection and shell breaking germination method |
CN107046853A (en) * | 2017-04-21 | 2017-08-18 | 王现明 | A kind of germination accelerating method of aspermous watermelon |
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2018
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Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2010200628A (en) * | 2009-02-27 | 2010-09-16 | Nanto Seed Co Ltd | Method for sterilizing seeds and seeds |
CN104969685A (en) * | 2014-04-09 | 2015-10-14 | 芜湖美农农业科技有限公司 | Production method of directly-seeding-able shell-breaking-free seedless watermelon seeds |
CN103988604A (en) * | 2014-04-23 | 2014-08-20 | 黄少伟 | Seedless watermelon seed germination accelerating method |
CN105723848A (en) * | 2014-12-06 | 2016-07-06 | 哈尔滨弘睿翔科技开发有限公司 | Watermelon seed selection and shell breaking germination method |
CN107046853A (en) * | 2017-04-21 | 2017-08-18 | 王现明 | A kind of germination accelerating method of aspermous watermelon |
Non-Patent Citations (1)
Title |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110692308A (en) * | 2019-10-14 | 2020-01-17 | 中国农业科学院郑州果树研究所 | Seedless watermelon shell-breaking-free germination accelerating method |
CN111149461A (en) * | 2020-01-21 | 2020-05-15 | 曹哲峰 | Cucurbitaceae seed shell treatment method |
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