CN105961201A - Tissue culture method for strawberry transplant seedlings - Google Patents
Tissue culture method for strawberry transplant seedlings Download PDFInfo
- Publication number
- CN105961201A CN105961201A CN201610320663.0A CN201610320663A CN105961201A CN 105961201 A CN105961201 A CN 105961201A CN 201610320663 A CN201610320663 A CN 201610320663A CN 105961201 A CN105961201 A CN 105961201A
- Authority
- CN
- China
- Prior art keywords
- culture
- seedling
- culture medium
- propagation
- carrying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a tissue culture method for strawberry transplant seedlings. The method comprises the steps: (A) selecting strawberry explants; (B) carrying out disinfection and sterilization; (C) carrying out anti-browning culture; (D) carrying out callus induction, bud generation and propagation culture; (E) carrying out propagation-expanding culture; (F) carrying out rooting culture; (G) carrying out seedling hardening and transplanting. The method has the beneficial effects that strawberry seedling tissue culture procedures are simple, and callus induction, bud generation and propagation culture can be simultaneously carried out in the same culture medium; the cycle of tissue culture is short, the propagation coefficient is high, and problems in browning phenomenon, callus, bud generation and propagation and rooting can be solved by only three kinds of culture media in a whole rapid propagation process; test-tube plantlet is subjected to adventitious root induction without adding plant hormones, so that the transplanting survival rate of hardened seedlings is high, and the growth speed is high; the seedlings can maintain the same genotype, so that propagated offspring are not prone to browning, and the viral accumulation is little; the browning inhibition tissue culture method is low in cost and short in time, the cultured seedlings are high in quality, high in survival rate and stable in traits, and thus the requirements of high-quality strawberry seedlings on industrial production can be met.
Description
Technical field
The invention belongs to seedling of Fructus Fragariae Ananssae technical field of tissue culture, relate to a kind of Fructus Fragariae Ananssae transplanted seedling tissue culture method.
Background technology
Fructus Fragariae Ananssae mainly breeds with stolon and plant division mode traditionally, and both modess of reproduction all exist the problems such as cycle length, coefficient be low.Long-term asexual propagation easily causes virus at strawberry cylinder accumulation, causes Fructus Fragariae Ananssae kind sexual involution, and yield reduces year by year.Utilize tissue culture technique to carry out detoxification, Fast-propagation is the effective way solving these problems.But in existing tissue culture technique, the overall applicability of tissue cultured seedling is in reduced levels, Plantlets of Strawberry Brown phenomenon is serious, breeding coefficient is low, group training cycle length, transplanting survival rate are low, have impact on the industrialized development of Fructus Fragariae Ananssae.
Summary of the invention
The present invention is directed in prior art, seedling of Fructus Fragariae Ananssae tissue culture technique exists that browning is serious, breeding coefficient is low, group training cycle length, problem that transplanting survival rate is low, it is provided that a kind of Fructus Fragariae Ananssae transplanted seedling tissue culture method, and its technical scheme is:
A kind of Fructus Fragariae Ananssae transplanted seedling tissue culture method, comprises the following steps:
A, the choosing of the outer implant of Fructus Fragariae Ananssae: select to grow fine, without pest and disease damage, without the healthy and strong strawberry of deformity, take its stolon stem-segment with node, be about 2 ~ 3 cm length with scalpel cutting, it is thus achieved that implant outside Fructus Fragariae Ananssae；
B, sterilization: outer implant is first cleaned with tap water earth and the dust on surface, then the washing powder solution that outer implant mass ratio is 10% is soaked 10 min, after slight concussion agitation, flowing water rinses 30 min, is placed in the vessel on superclean bench；3 min are soaked again with the vitamin c solution that mass ratio is 50%, it is that 75% ethanol solution processes 10 ~ 15 s by volume ratio, then through hydrargyrum aqueous solution sterilization 8 ~ 10 min that mass ratio is 0.1%, finally uses aseptic water washing 5 ~ 6 times, it is not less than 3 min every time, whole disinfecting process fully shakes vessel；
C, anti-brownization are cultivated: add the sodium thiosulfate of 20000 ~ 30000 mg in every liter of distilled water, the vitamin C of 100 ~ 150 mg, the activated carbon of 1.0 ~ 5.0mg, the sodium chloride of 1.0 ~ 10 mg, the sucrose of 30000 mg and the agar powder of 5300 mg are configured to culture medium I, and the pH value of culture medium I is between 5.4 ~ 5.8；
The outer implant of sterilized sterilizing being accessed in culture medium I and cultivate, after being spaced 7 d, be transferred to outer implant in another identical fresh culture I cultivate, outer implant corotation connects 3 times and completes anti-brownization process；
D, callus induction, clump bud occurs and enrichment culture: add the 6-benzamido group purine of 1.0 ~ 1.5 mg in every liter of distilled water, the naphthalene acetic acid of 0.1 ~ 1.0 mg, the 2 of 0.05 ~ 0.1 mg, 4-dichlorphenoxyacetic acid, the sucrose of 30000 mg, the agar of 5300 ~ 5500 mg makes culture medium II, and the pH value of culture medium II is between 5.4 ~ 5.8；
The outer implant obtained in C is linked in culture medium II, being 1500 ~ 2000 lx in illuminance, light application time is 12 h/d, and temperature controls to carry out callus induction under conditions of 22 ± 1 DEG C, clump bud occurs and enrichment culture, callus occurs in its joint portion and base portion after 5 d；After 10 d, callus is bred rapidly, and its surface starts green bud point occur；Adventitious bud clump is differentiated after 15 d；
E, expanding propagation are cultivated: carrying out aseptic cutting by differentiating adventitious bud clump after 15 d in D step, be cut into 4-6 strain one clump, be transferred to respectively in another identical fresh culture II, carry out expanding propagation, after cultivating 30 d, expanding propagation turns out seedling；
F, root culture: add the activated carbon of 1.0 mg, the sucrose of 10000 mg in every liter of distilled water, the agar powder of 5300-5500 mg is configured to culture medium III, and the pH value of culture medium III is between 5.4 ~ 5.8；
Taking the healthy and strong seedling of high 3 ~ 4 cm in E step, be inoculated in culture medium III, being placed on illuminance is 1500 ~ 2000 lx, and light application time is 12 h/d, and temperature controls to cultivate under conditions of 22 ± 1 DEG C, obtains Seedling and strengthen the Seedling of taking root that root is thick after cultivating 45 d；
G, seedling exercising and transplanting: take the high Seedling of taking root of 6 ~ 8 cm, after being placed in room temperature lower refining seedling 3 d together with culture bottle, open bottle cap, seedling is taken out from culture medium, remaining medium on seedling is cleaned up, after putting into 2 ~ 3 min that sterilize in the carbendazim solution that concentration is 0.1 ~ 0.2%, transplants heat and moisture preserving in the sandy soil substrate to sterilization and cultivate, after growing 30 d, obtain transplanted seedling.
The medicine have the advantages that
1, whole year production can be realized in culturing room with tissue culture technique, both save land resource, and improve again economic benefit, overcome the difficult point that tradition modes of reproduction cannot carry out producing in the anniversary.
2, solve the browning easily occurred in strawberry plants Fast-propagation, plant shoot survival percent and quality is improved.
3, eliminate the outer implant after brownization to synchronize to carry out wound healing group induction, the differentiation of adventitious bud clump in same culture medium, substantially reduce the vitro propagation cycle of Fructus Fragariae Ananssae, solve forefathers and fail to synchronize callus induction and the problem of bud clump differentiation.
4, all seedlings can keep same gene type background, it is prone to standardization, batch production operation, it is effectively improved seedling quality, can be that spread plantation provides the good seed sought unity of standard, solve raise up seed easy brownization, the virus accumulation of tradition Fructus Fragariae Ananssae many, Character instability and a difficult problem that the seedling quality that causes differs.
5, test tube Seedling root induction is not added with phytohormone, and acclimatization and transplants survival rate is high, and growth is rapidly, and effect is fine.
6, using three kinds of culture medium to carry out anti-brownization respectively to process, callus, clump bud occur and propagation processes and rooting treatment, solve the technical problem that seedling of Fructus Fragariae Ananssae different growing stage exists, and solve the with strong points, effective of problem.
The method of the Browning control 7, used, low cost, the time is short, the Seedling quality better that brings out and survival rate high, merit can be fixed up, the factorial praluction demand of Fructus Fragariae Ananssae high-quality seedling can be met.
Detailed description of the invention
Embodiment one: a kind of Fructus Fragariae Ananssae transplanted seedling tissue culture method, comprises the following steps:
A, the choosing of the outer implant of Fructus Fragariae Ananssae: select to grow fine, without pest and disease damage, without the healthy and strong strawberry of deformity, take its stolon stem-segment with node, be about 3 cm length with scalpel cutting, it is thus achieved that implant outside Fructus Fragariae Ananssae；
B, sterilization: outer implant is first cleaned with tap water earth and the dust on surface, then the washing powder solution that outer implant mass ratio is 10% is soaked 10 min, after slight concussion agitation, flowing water rinses 30 min, is placed in the vessel on superclean bench；3 min are soaked again with the vitamin c solution that mass ratio is 50%, it is that 75% ethanol solution processes 13 s by volume ratio, then sterilizes 9 min through the hydrargyrum aqueous solution that mass ratio is 0.1%, finally with aseptic water washing 5 times, each 4 min, fully shake vessel in whole disinfecting process；
C, anti-brownization are cultivated: add the sodium thiosulfate of 25 mg in every liter of distilled water, the vitamin C of 130 mg, the activated carbon of 3.0 mg, the sodium chloride of 5 mg, the sucrose of 30000 mg and the agar powder of 5300 mg are configured to culture medium I, and the pH value of culture medium I is 5.6；
The outer implant of sterilized sterilizing being accessed in culture medium I and cultivate, after being spaced 7 d, be transferred to outer implant in another identical fresh culture I cultivate, outer implant corotation connects 3 times and completes anti-brownization process；
D, callus induction, clump bud occurs and enrichment culture: add the 6-benzamido group purine of 1.3 mg, the naphthalene acetic acid of 0.5 mg in every liter of distilled water, the 2 of 0.08 mg, 4-dichlorphenoxyacetic acid, the sucrose of 30000 mg, the agar of 5400 mg makes culture medium II, and the pH value of culture medium II is 5.6；
The outer implant obtained in C being linked in culture medium II, be 1800 lx in illuminance, light application time is 12 h/d, temperature controls to carry out callus induction under conditions of 22 DEG C, clump bud occurs and enrichment culture, callus occurs in its joint portion and base portion after 5 d, and obtaining the rate of healing is 98.87%；After 10 d, callus is bred rapidly, and its surface starts green bud point occur；Adventitious bud clump, bud clump differentiation rate 100% is differentiated after 15 d；20 d metaplexus bud genetic coefficients are up to 6.32；
E, expanding propagation are cultivated: carrying out aseptic cutting by differentiating adventitious bud clump after 15 d in D step, be cut into 5 strain one clump, be transferred to respectively in another identical fresh culture II, carry out expanding propagation, after cultivating 30 d, average breeding coefficient reaches 12.86, and expanding propagation turns out seedling；
F, root culture: add the activated carbon of 1.0 mg, the sucrose of 10000 mg in every liter of distilled water, the agar powder of 5400 mg is configured to culture medium III, and the pH value of culture medium III is 5.6；
Taking the healthy and strong seedling of high 4 cm in E step, be inoculated in culture medium III, being placed on illuminance is 1800 lx, and light application time is 12 h/d, and temperature controls to cultivate under conditions of 22 DEG C, obtains Seedling and strengthen the Seedling of taking root that root is thick after cultivating 45 d, and its rooting rate is up to 100%；
G, seedling exercising and transplanting: take the high Seedling of taking root of 7 cm, after being placed in room temperature lower refining seedling 3 d together with culture bottle, open bottle cap, from culture medium, take out seedling, remaining medium on seedling is cleaned up, after putting into 3 min that sterilize in the carbendazim solution that concentration is 0.15%, transplant heat and moisture preserving in the sandy soil substrate to sterilization to cultivate, after growing 30 d, obtaining transplanted seedling, survival rate is up to 98%.
Claims (1)
1. Fructus Fragariae Ananssae transplanted seedling tissue culture method, it is characterised in that comprise the following steps:
A, the choosing of the outer implant of Fructus Fragariae Ananssae: select to grow fine, without pest and disease damage, without the healthy and strong strawberry of deformity, take its stolon stem-segment with node, be about 2 ~ 3 cm length with scalpel cutting, it is thus achieved that implant outside Fructus Fragariae Ananssae；
B, sterilization: outer implant is first cleaned with tap water earth and the dust on surface, then the washing powder solution that outer implant mass ratio is 10% is soaked 10 min, after slight concussion agitation, flowing water rinses 30 min, is placed in the vessel on superclean bench；3 min are soaked again with the vitamin c solution that mass ratio is 50%, it is that 75% ethanol solution processes 10 ~ 15 s by volume ratio, then through hydrargyrum aqueous solution sterilization 8 ~ 10 min that mass ratio is 0.1%, finally uses aseptic water washing 5 ~ 6 times, it is not less than 3 min every time, whole disinfecting process fully shakes vessel；
C, anti-brownization are cultivated: add the sodium thiosulfate of 20000 ~ 30000 mg in every liter of distilled water, the vitamin C of 100 ~ 150 mg, the activated carbon of 1.0 ~ 5.0mg, the sodium chloride of 1.0 ~ 10 mg, the sucrose of 30000 mg and the agar powder of 5300 mg are configured to culture medium I, and the pH value of culture medium I is between 5.4 ~ 5.8；
The outer implant of sterilized sterilizing being accessed in culture medium I and cultivate, after being spaced 7 d, be transferred to outer implant in another identical fresh culture I cultivate, outer implant corotation connects 3 times and completes anti-brownization process；
D, callus induction, clump bud occurs and enrichment culture: the 6-benzamido group purine of addition 1.0 ~ 1.5 mg in every liter of distilled water, and 0.1 ~ 1.0
The naphthalene acetic acid of mg, 0.05 ~ 0.1
The 2 of mg, 4-dichlorphenoxyacetic acid, the sucrose of 30000 mg, the agar of 5300 ~ 5500 mg makes culture medium II, and the pH value of culture medium II is between 5.4 ~ 5.8；
The outer implant obtained in C is linked in culture medium II, being 1500 ~ 2000 lx in illuminance, light application time is 12 h/d, and temperature controls to carry out callus induction under conditions of 22 ± 1 DEG C, clump bud occurs and enrichment culture, callus occurs in its joint portion and base portion after 5 d；After 10 d, callus is bred rapidly, and its surface starts green bud point occur；Adventitious bud clump is differentiated after 15 d；
E, expanding propagation are cultivated: carrying out aseptic cutting by differentiating adventitious bud clump after 15 d in D step, be cut into 4-6 strain one clump, be transferred to respectively in another identical fresh culture II, carry out expanding propagation, after cultivating 30 d, expanding propagation turns out seedling；
F, root culture: add the activated carbon of 1.0 mg, the sucrose of 10000 mg in every liter of distilled water, the agar powder of 5300-5500 mg is configured to culture medium III, and the pH value of culture medium III is between 5.4 ~ 5.8；
Taking the healthy and strong seedling of high 3 ~ 4 cm in E step, be inoculated in culture medium III, being placed on illuminance is 1500 ~ 2000 lx, and light application time is 12 h/d, and temperature controls to cultivate under conditions of 22 ± 1 DEG C, obtains Seedling and strengthen the Seedling of taking root that root is thick after cultivating 45 d；
G, seedling exercising and transplanting: take the high Seedling of taking root of 6 ~ 8 cm, after being placed in room temperature lower refining seedling 3 d together with culture bottle, open bottle cap, seedling is taken out from culture medium, remaining medium on seedling is cleaned up, after putting into 2 ~ 3 min that sterilize in the carbendazim solution that concentration is 0.1 ~ 0.2%, transplants heat and moisture preserving in the sandy soil substrate to sterilization and cultivate, after growing 30 d, obtain transplanted seedling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610320663.0A CN105961201A (en) | 2016-05-16 | 2016-05-16 | Tissue culture method for strawberry transplant seedlings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610320663.0A CN105961201A (en) | 2016-05-16 | 2016-05-16 | Tissue culture method for strawberry transplant seedlings |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105961201A true CN105961201A (en) | 2016-09-28 |
Family
ID=56955431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610320663.0A Pending CN105961201A (en) | 2016-05-16 | 2016-05-16 | Tissue culture method for strawberry transplant seedlings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105961201A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107182785A (en) * | 2017-06-16 | 2017-09-22 | 黔东南民族职业技术学院 | Strawberry tissue culture method |
| CN109220790A (en) * | 2018-08-30 | 2019-01-18 | 丽江市古城区秋成种养殖有限公司 | A kind of in vitro outer breeding method of red fruit ginseng |
| CN111528090A (en) * | 2020-05-06 | 2020-08-14 | 天津农学院 | Strawberry tissue culture seedling hardening matrix for improving hardening quality of strawberry tissue culture seedlings and preparation and use methods and application thereof |
| CN111955346A (en) * | 2020-09-03 | 2020-11-20 | 云南华农农业有限公司 | Novel method for inhibiting browning and improving artificial rapid propagation efficiency of Monte raspberries |
| CN112586350A (en) * | 2020-12-02 | 2021-04-02 | 蚌埠海上明珠农业科技发展有限公司 | Strawberry virus-free seedling culture method |
| CN116686708A (en) * | 2023-06-13 | 2023-09-05 | 武汉生物工程学院 | Method for open tissue culture of strawberry virus-free seedlings |
| CN118696832A (en) * | 2024-08-27 | 2024-09-27 | 云南省农业科学院药用植物研究所 | Tissue culture method of medicinal plant fructus psoraleae |
| CN118696832B (en) * | 2024-08-27 | 2024-10-25 | 云南省农业科学院药用植物研究所 | Tissue culture method of medicinal plant fructus psoraleae |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05153996A (en) * | 1991-11-01 | 1993-06-22 | Ishikawajima Harima Heavy Ind Co Ltd | Production of anthocyanin using cultured cell of strawberry |
| CN101803569A (en) * | 2010-03-26 | 2010-08-18 | 通化师范学院 | Method for inducing strawberry stolons and eliminating viruses in test tube by high temperature processing in combination with shoot tip culture |
| CN103004754A (en) * | 2012-12-14 | 2013-04-03 | 苏州和美生物科技有限公司 | Composition for preventing browning of plant tissue culture and using method of composition |
| CN103299905A (en) * | 2013-05-31 | 2013-09-18 | 句容茂园生态农业发展有限公司 | Strawberry subculture method |
| CN104186351A (en) * | 2014-09-24 | 2014-12-10 | 江苏农林职业技术学院 | Tissue culture method of strawberries |
| CN104686323A (en) * | 2014-12-31 | 2015-06-10 | 四川省农业科学院园艺研究所 | Method for cultivating strawberry seedlings by secondary detoxification method |
-
2016
- 2016-05-16 CN CN201610320663.0A patent/CN105961201A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05153996A (en) * | 1991-11-01 | 1993-06-22 | Ishikawajima Harima Heavy Ind Co Ltd | Production of anthocyanin using cultured cell of strawberry |
| CN101803569A (en) * | 2010-03-26 | 2010-08-18 | 通化师范学院 | Method for inducing strawberry stolons and eliminating viruses in test tube by high temperature processing in combination with shoot tip culture |
| CN103004754A (en) * | 2012-12-14 | 2013-04-03 | 苏州和美生物科技有限公司 | Composition for preventing browning of plant tissue culture and using method of composition |
| CN103299905A (en) * | 2013-05-31 | 2013-09-18 | 句容茂园生态农业发展有限公司 | Strawberry subculture method |
| CN104186351A (en) * | 2014-09-24 | 2014-12-10 | 江苏农林职业技术学院 | Tissue culture method of strawberries |
| CN104686323A (en) * | 2014-12-31 | 2015-06-10 | 四川省农业科学院园艺研究所 | Method for cultivating strawberry seedlings by secondary detoxification method |
Non-Patent Citations (2)
| Title |
|---|
| 牛红云等: "草莓（Fragaria ananassa Duch）茎段组织培养的研究", 《东北农业大学学报》 * |
| 郭艳等: "植物组织培养中的褐化现象及解决途径", 《山西农业科学》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107182785A (en) * | 2017-06-16 | 2017-09-22 | 黔东南民族职业技术学院 | Strawberry tissue culture method |
| CN109220790A (en) * | 2018-08-30 | 2019-01-18 | 丽江市古城区秋成种养殖有限公司 | A kind of in vitro outer breeding method of red fruit ginseng |
| CN111528090A (en) * | 2020-05-06 | 2020-08-14 | 天津农学院 | Strawberry tissue culture seedling hardening matrix for improving hardening quality of strawberry tissue culture seedlings and preparation and use methods and application thereof |
| CN111955346A (en) * | 2020-09-03 | 2020-11-20 | 云南华农农业有限公司 | Novel method for inhibiting browning and improving artificial rapid propagation efficiency of Monte raspberries |
| CN112586350A (en) * | 2020-12-02 | 2021-04-02 | 蚌埠海上明珠农业科技发展有限公司 | Strawberry virus-free seedling culture method |
| CN116686708A (en) * | 2023-06-13 | 2023-09-05 | 武汉生物工程学院 | Method for open tissue culture of strawberry virus-free seedlings |
| CN118696832A (en) * | 2024-08-27 | 2024-09-27 | 云南省农业科学院药用植物研究所 | Tissue culture method of medicinal plant fructus psoraleae |
| CN118696832B (en) * | 2024-08-27 | 2024-10-25 | 云南省农业科学院药用植物研究所 | Tissue culture method of medicinal plant fructus psoraleae |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105961201A (en) | Tissue culture method for strawberry transplant seedlings | |
| CN101491215B (en) | Chinese toon tissue-culture quick propagation technique | |
| CN103190347B (en) | Teapot dates tissue culturing method | |
| CN105494103A (en) | Method for tissue culture and rapid propagation of high-quality paphiopedilum maudiae seedlings | |
| CN107182780A (en) | The method for culturing seedlings of P. kingianum | |
| CN105638477A (en) | Rapid propagation method for dendrobium hancockii seeds | |
| CN111789027B (en) | Method for simultaneously and efficiently obtaining cluster buds and rooted seedlings by taking beautiful bamboo rhizome buds as explants | |
| CN109258460A (en) | Micro-stem tip culture combines the breeding method of heat treatment acquisition Zengcheng honey chrysanthemum detoxic seedling | |
| CN101897297B (en) | Two-step tissue culture quick propagation method for hemerocallis | |
| CN102047842A (en) | Method for directly regenerating plants by adopting citrullus lanatus cotyledon nodes | |
| CN105265316B (en) | A kind of allium plateau rapid propagation method | |
| CN115281081A (en) | Breeding method of miniature test tube detoxified seed ginger | |
| CN103155862B (en) | Sinocalamus latiflorus flower pesticide inductor embryo the method obtaining regeneration plant | |
| CN106106178B (en) | A kind of method for tissue culture of candy iris | |
| CN106069755A (en) | A kind of strengthening seedling and rooting cultural method of tea-tree tissue culture seedling | |
| CN105265317B (en) | A kind of For-carrying green onions rapid propagation method | |
| CN104938335B (en) | The method that regeneration plant is obtained using oil tea hypocotyls | |
| CN100394845C (en) | In-bottle production method of detoxified small seed ball of east lily | |
| CN103155869A (en) | Sweet cherry rootstock Colt tissue culture method | |
| CN105766639B (en) | A kind of method of cultivating sweet sorghum tissue culture fast seedling growing | |
| CN115606503B (en) | Tissue culture method of aster | |
| CN104012406A (en) | Regeneration in-vitro method for sweet cherry variety wanhongzhu | |
| CN110476816A (en) | A method of being quickly obtained grape paulownia seedling | |
| CN105409773A (en) | Lophophora williamsii sterile seeding and regeneration system establishing method | |
| CN106613973B (en) | Utilize the method for tissue-cultured seedling leaf regeneration adventitious bud fast breeding Chinese azalea |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20160926 Address after: 653100 Yuxi City, Yunnan province Hongta District North Street Community Lianchi nine group thirty-two Building No. six Applicant after: Yuxi Xiang Xin agricultural technology development Co., Ltd. Address before: 653100 No. 7, No. 8, Caixin Road, hi tech Zone, Yunnan, Yuxi Applicant before: YUXI LIUHE AGRICULTURAL SCIENCE AND TECHNOLOGY DEVELOPMENT CO., LTD. |
|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160928 |