CN108370808A - A kind of Mycorrhizal Fungi of Pinus massoniana seedling method for culturing seedlings - Google Patents
A kind of Mycorrhizal Fungi of Pinus massoniana seedling method for culturing seedlings Download PDFInfo
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- CN108370808A CN108370808A CN201810260003.7A CN201810260003A CN108370808A CN 108370808 A CN108370808 A CN 108370808A CN 201810260003 A CN201810260003 A CN 201810260003A CN 108370808 A CN108370808 A CN 108370808A
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- seedling
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- bacterium solution
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/10—Mycorrhiza; Mycorrhizal associations
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of Mycorrhizal Fungi of Pinus massoniana seedling method for culturing seedlings, this method includes that seed disinfection cuts root processing and bacterium solution inoculation and five steps of transplanting and management with vernalization, seedling medium disinfection, strain acquisition and bacterium solution preparation, seedling.The present invention is easy to get the Mycorrhizal seedling of purpose strain, and ensure that its infection rate, and operating process of the present invention is simple and convenient, and effect of inoculation is very ideal, and infection rate is up to 95% or more；It is simple to cut root process, it is convenient and efficient, it is smaller to seedling injury；Germination box of the present invention and net are every can recycle, economical environment-protective.
Description
Technical field
The present invention relates to a kind of Mycorrhizal Fungi of Pinus massoniana seedling method for culturing seedlings, belong to Mycorrhizal seedling seedling-raising technique field.
Background technology
Masson pine (Pinus massoniana Lamb.) be cultivated area is maximum, distribution is most wide in China's coniferous species,
One of full tree highest indigenous tree of comprehensive utilization degree, and the most important industrial cut stock seeds in south, have important warp
Ji and the ecological value, belong to typical exotrophic mycorrhiza seeds.Exotrophic mycorrhiza can promote absorption of the plant to mineral nutrition, enhancing
The resistance etc. of plant, and then promote growth.But in practical nursery is operated in, the Mycorrhizal masson pine of target strain is obtained
Seedling is extremely difficult.
Exotrophic mycorrhiza is the homobium that Applying Ectomycorrhizal Fungi is formed with masson pine young root, has extension mycelia, bacterium set, Ha Di
The structures such as family name's net, can promote masson pine to enhance its resistance to the absorption of nutriment.Traditional mycorrhizal seedlings cultivation is adopted
It is formd in practice process with dressing seed, mixing the cultivation that the modes such as soil, root dipping carry out mycorrhizal seedlings and cuts root method cultivation horse hair
Loose Mycorrhizal seedling technology.This technology is mainly carried out in three steps, and masson pine seed is seeded in matrix first, waits for masson pine
After growth of seedling 15 days, seedling is removed successively and cuts out 1-2 centimetres of young root, will finally cut a root seedling point and dip in Applying Ectomycorrhizal Fungi
It is moved into soil hole after culture solution.But this process is cumbersome to be taken, and the process of nursery stock transfer is larger to seedling injury, shadow
Ring the further development of nursery stock.Therefore, it is necessary to study a kind of conveniently Mycorrhizal Fungi of Pinus massoniana seedling culture methods, thus
Improve breeding efficiency.
Invention content
In order to solve the above technical problem, the present invention provides a kind of Mycorrhizal Fungi of Pinus massoniana seedling method for culturing seedlings, this method makes
It is more convenient simple to obtain Mycorrhizal Fungi of Pinus massoniana seedling nursery, solves and cuts root process in masson pine target strain Va Mycorrhiza Seedling seedling raising process
Cumbersome, the problems such as Va Mycorrhiza Seedling survival rate is low, infection rate is low.
Technical scheme of the present invention：A kind of Mycorrhizal Fungi of Pinus massoniana seedling method for culturing seedlings, includes the following steps：
(1) seed disinfection and vernalization：Seed after wet concentration is impregnated into 2h with 0.5% potassium permanganate, pulls out and is placed on burning
Diaphragm seal stands 30 minutes on cup middle cover, with aseptic water washing 3~4 times；The seed after disinfection, which is placed in initial temperature, again is
It in 45 DEG C of sterile water, is put into 25 DEG C of growth cabinets and soaks seed for 24 hours, change a water every 6~8h halfway, with wet yarn after seed soaking
Cloth package seed is placed in vernalization in the growth cabinet that temperature is 25 DEG C, and waiting for that seed shows money or valuables one carries unintentionally can sow；
(2) seedling medium sterilizes：Seedling medium sterilizes 2h postcoolings through high-pressure steam sterilizing pan at a temperature of 121 DEG C,
It can seeding and seedling raising；
(3) strain is obtained prepares with bacterium solution：Applying Ectomycorrhizal Fungi is taken out from solid medium and is inoculated in liquid training
It supports in base, it is spare after being cultivated 15 days in growth cabinet；
(4) seedling cuts root processing and is inoculated with bacterium solution：By seedling medium be fitted into germination box at height 1cm, be put into net every
After continue add seedling medium to from germination box edge 1cm at, sowed after sprinkling profoundly water, cover matrix to cover seed, no
It is preferably blocked up, being placed in growth cabinet for sowing is completed, gives suitable illumination and humidity, and moisture is replenished in time, until
Seed throws off box cover after being unearthed；By net every integrally being lifted with seedling together with matrix after sowing 15 days, by exposing net every root it is parallel
It is cut away in bottom, while net pours into Applying Ectomycorrhizal Fungi bacterium solution every the bottom faces Qie Gen and seedling medium contact surface in the box that germinates,
By net every be put into germination box in continue cultivate 2 weeks in growth cabinet after transplant；
(5) transplanting and management：Will inoculation seedling from germination box in extract and put dip in bacterium solution after be moved into the hole tray of nursery, together
When keep soil moisture humidity, give suitable illumination, prevent weeds propagation and widespread, prevention pest and disease damage infringement.
In the above method, the Applying Ectomycorrhizal Fungi source is that the fructification of masson pine forest acquisition is isolated.
In the above method, the solid medium is by ammonium tartrate 0.5g/L, potassium dihydrogen phosphate 1.0g/L, magnesium sulfate
0.5g/L, glucose 20g/L, vitaminB10 .1 × 10-3G/L, agar 20g/L, boric acid 8.45mg/L, manganese sulfate 5mg/L, sulphur
Sour copper 0.625mg/L, ferrous sulfate 6mg/L, zinc chloride 2.77mg/L and ammonium molybdate 0.27mg/L are composed, wherein liquid
Culture medium prescription is consistent with solid medium, but does not add agar.
Due to the adoption of the above technical scheme, the advantage of the invention is that：
First, the present invention is easy to get the Mycorrhizal seedling of purpose strain, and it ensure that its infection rate.Operating process of the present invention
Simple and convenient, effect of inoculation is very ideal, and infection rate is up to 95% or more；
Second, it is simple to cut root process, and it is convenient and efficient, it is smaller to seedling injury；
Third, germination box of the present invention and net be every can recycle, economical environment-protective.
Description of the drawings
Fig. 1 is the structural schematic diagram of germination box in the present invention；
Fig. 2 be in the present invention net every structural schematic diagram.
Specific implementation mode
To make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and examples to this hair
It is bright to be described in further detail.
The embodiment of the present invention：A kind of Mycorrhizal Fungi of Pinus massoniana seedling method for culturing seedlings, includes the following steps：
(1) the preferable seed of quality is selected after wet concentration with being pulled out after 5% potassium permanganate disinfection 2h, is placed in beaker middle cover
Upper diaphragm seal stands 30 minutes, is placed in the sterile water that initial temperature is 45 DEG C for 3 times with aseptic water washing, is then placed in again
It soaks seed for 24 hours in the growth cabinet that temperature is 25 DEG C or so, changes a water per 6h halfway, set with wet gauze package seed after seed soaking
The vernalization in the growth cabinet that temperature is 25 DEG C, is sowed after seed shows money or valuables one carries unintentionally.
(2) the 2h postcoolings that seedling medium high-pressure steam sterilizing pan sterilize at a temperature of 121 DEG C are spare.In the present invention
The structural schematic diagram of germination box is as shown in Figures 1 and 2, it includes germination box 1 and box cover 2, and the germination box 1 is one upper wide
Under narrow inverted trapezoidal box body, net is placed in germination box 1 every 3, net is identical as germination box 1 every 3 shape, and volume is less than and sends out
Bud box 1.In use, after being packed into the seedling medium of 1cm thickness in the box 1 that germinates, net is put into every 3 in germination box 1 and continues to add
It at seedling medium to 1 edge 1cm of germination box, is sowed after matrix is sprinkled profoundly water, per 200~300 seeds of box.Complete sowing
Afterwards, box cover 2 is covered, germination box 1 is placed in growth cabinet, suitable illumination and humidity is given, and moisture is replenished in time, waits for
Box cover 2 is thrown off after being unearthed to seed.
(3) will be acquired under masson pine forest isolated Applying Ectomycorrhizal Fungi activation, expand it is numerous.It is first that exotrophic mycorrhiza is true
Bacterium is inoculated on Pachlewski solid mediums, spare after being cultivated 20 days in growth cabinet.The solid medium
It is by ammonium tartrate 0.5g/L, potassium dihydrogen phosphate 1.0g/L, magnesium sulfate 0.5g/L, glucose 20g/L, vitaminB10 .1 × 10- 3G/L, agar 20g/L, boric acid 8.45mg/L, manganese sulfate 5mg/L, copper sulphate 0.625mg/L, ferrous sulfate 6mg/L, zinc chloride
2.77mg/L and ammonium molybdate 0.27mg/L compositions.Wherein Liquid Culture based formulas is consistent with solid medium, but does not add fine jade
Fat.Bacterium solution preparation process：250ml triangular flasks are taken to be packed into 150ml fluid nutrient mediums, the temperature with high-pressure steam sterilizing pan at 121 DEG C
The lower 30 minutes postcoolings that sterilize of degree, from taking fungus block to be inoculated in fluid nutrient medium on solid medium, entire seeded process is super
Net workbench is completed.Triangular flask is placed in growth cabinet and cultivates 20~25 days spare, 25 DEG C or so of temperature, humidity adaptation is
It can.
(4) net is integrally lifted together with seedling medium with seedling after sowing 15 days every 3, masson pine of the net every 3 will be exposed
The young root of seedling is parallel to net and is cut away every 3 bottoms.
(5) 100ml Applying Ectomycorrhizal Fungi bacterium are poured into every the 3 bottom faces Qie Gen and seedling medium contact surface in germination box in net
Liquid moves the net containing matrix and seedling every 3 whole are put into after continuation cultivates 2 weeks in growth cabinet in germination box
It plants.Carefully seedling is extracted when transplanting, point moves into after dipping in bacterium solution of the same race in hole tray.
(6) management process mainly keeps soil moisture humidity, prevents weeds propagation and widespread, prevents the infringement of pest and disease damage in time, complete
It can be obtained the Mycorrhizal seedling of purpose strain at whole process, whole process is in progress at the beginning of 3 months.
Claims (3)
1. a kind of Mycorrhizal Fungi of Pinus massoniana seedling method for culturing seedlings, it is characterised in that include the following steps：
(1) seed disinfection and vernalization：Seed after wet concentration is impregnated into 2h with 0.5% potassium permanganate, pulls out and is placed in beaker
It covers diaphragm seal and stands 30 minutes, with aseptic water washing 3~4 times；It is 45 DEG C that the seed after disinfection, which is placed in initial temperature, again
Sterile water in, be put into 25 DEG C of growth cabinets and soak seed for 24 hours, change a water every 6~8h halfway, with wet gauze packet after seed soaking
It wraps up in seed and is placed in vernalization in the growth cabinet that temperature is 25 DEG C, waiting for that seed shows money or valuables one carries unintentionally can sow；
(2) seedling medium sterilizes：Seedling medium sterilizes 2h postcoolings through high-pressure steam sterilizing pan at a temperature of 121 DEG C, you can
Seeding and seedling raising；
(3) strain is obtained prepares with bacterium solution：Applying Ectomycorrhizal Fungi is taken out from solid medium and is inoculated in fluid nutrient medium
In, it is spare after being cultivated 15 days in growth cabinet；
(4) seedling cuts root processing and is inoculated with bacterium solution：Seedling medium is fitted into germination box to height 1cm, is put into net every subsequent
Continuous addition seedling medium is sowed at from germination box edge 1cm after sprinkling profoundly water, and covers matrix to cover seed, should not mistake
Thickness completes being placed in growth cabinet for sowing, gives suitable illumination and humidity, and moisture is replenished in time, until seed
Box cover is thrown off after being unearthed；By net every integrally being lifted with seedling together with matrix after sowing 15 days, by exposing net every root be parallel to bottom
Portion is cut away, while net pours into Applying Ectomycorrhizal Fungi bacterium solution every the bottom faces Qie Gen and seedling medium contact surface in the box that germinates, by net
It is transplanted every being put into after continuation cultivates 2 weeks in growth cabinet in germination box；
(5) transplanting and management：Will inoculation seedling from germination box in extract and put dip in bacterium solution after be moved into the hole tray of nursery, protect simultaneously
Soil moisture humidity is held, suitable illumination is given, prevents weeds propagation and widespread, prevention pest and disease damage infringement.
2. Mycorrhizal Fungi of Pinus massoniana seedling method for culturing seedlings according to claim 1, it is characterised in that：The Applying Ectomycorrhizal Fungi
Source is that the fructification of masson pine forest acquisition is isolated.
3. Mycorrhizal Fungi of Pinus massoniana seedling method for culturing seedlings according to claim 1, it is characterised in that：The solid medium is
By ammonium tartrate 0.5g/L, potassium dihydrogen phosphate 1.0g/L, magnesium sulfate 0.5g/L, glucose 20g/L, vitaminB10 .1 × 10-3g/
L, agar 20g/L, boric acid 8.45mg/L, manganese sulfate 5mg/L, copper sulphate 0.625mg/L, ferrous sulfate 6mg/L, zinc chloride
2.77mg/L and ammonium molybdate 0.27mg/L are composed, and wherein Liquid Culture based formulas is consistent with solid medium, but does not add
Add agar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810260003.7A CN108370808B (en) | 2018-03-27 | 2018-03-27 | Pinus massoniana mycorrhizal seedling raising method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810260003.7A CN108370808B (en) | 2018-03-27 | 2018-03-27 | Pinus massoniana mycorrhizal seedling raising method |
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| CN108370808A true CN108370808A (en) | 2018-08-07 |
| CN108370808B CN108370808B (en) | 2020-06-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810260003.7A Active CN108370808B (en) | 2018-03-27 | 2018-03-27 | Pinus massoniana mycorrhizal seedling raising method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110915546A (en) * | 2019-12-17 | 2020-03-27 | 云南大学 | Method for promoting plant to form ectomycorrhiza |
| CN111034557A (en) * | 2019-12-19 | 2020-04-21 | 扬州大学 | Seedling for indoor inoculation of barley yellow mosaic disease and culture method of seedling after inoculation |
| CN112075236A (en) * | 2020-09-23 | 2020-12-15 | 贵州大学 | Method for promoting secondary coniferous germination of annual pinus massoniana seedlings |
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| CN105393873A (en) * | 2015-11-11 | 2016-03-16 | 黎平县枞集林农发展有限责任公司 | Cultivation method for masson pine seedlings |
| CN106134904A (en) * | 2016-07-28 | 2016-11-23 | 杭州凌萤科技有限公司 | Pinus massoniana Lamb fast seedling-cultivating method |
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| CN102732446A (en) * | 2012-04-16 | 2012-10-17 | 南京林业大学 | Brevibacillusreuszeri and application thereof in promoting pine tree growth |
| CN103460941A (en) * | 2013-09-16 | 2013-12-25 | 广东省林业科学研究院 | Method for cultivating semi-annual high-rosin pinus massoniana lamb seedlings in nutrient bags |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110915546A (en) * | 2019-12-17 | 2020-03-27 | 云南大学 | Method for promoting plant to form ectomycorrhiza |
| CN110915546B (en) * | 2019-12-17 | 2021-11-02 | 云南大学 | Method for promoting plant to form ectomycorrhiza |
| CN111034557A (en) * | 2019-12-19 | 2020-04-21 | 扬州大学 | Seedling for indoor inoculation of barley yellow mosaic disease and culture method of seedling after inoculation |
| CN111034557B (en) * | 2019-12-19 | 2022-04-19 | 扬州大学 | Seedling for indoor inoculation of barley yellow mosaic disease and culture method of seedling after inoculation |
| CN112075236A (en) * | 2020-09-23 | 2020-12-15 | 贵州大学 | Method for promoting secondary coniferous germination of annual pinus massoniana seedlings |
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| CN108370808B (en) | 2020-06-12 |
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