CN104396707A - Method for improving stress resistance of pinus sylvestris seedling - Google Patents
Method for improving stress resistance of pinus sylvestris seedling Download PDFInfo
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- CN104396707A CN104396707A CN201410275883.7A CN201410275883A CN104396707A CN 104396707 A CN104396707 A CN 104396707A CN 201410275883 A CN201410275883 A CN 201410275883A CN 104396707 A CN104396707 A CN 104396707A
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- inoculation
- microbial inoculum
- sterilizing
- improved
- green trichoderma
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Environmental Sciences (AREA)
- Mycology (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention relates to a method for improving stress resistance of a pinus sylvestris seedling. The method comprises the steps of: (1) mycelium fermentation broth culturing; (2) inoculant preparation; (3) seed treatment; (4) sterile soil preparation; (5) sowing; and (6) inoculant inoculation. With the method provided by the invention, pinus sylvestris seedling growth and stress resistance levels are improved. With a drilling and root irrigation manner and an interval inoculation manner, a suillus luteus fermentation broth is inoculated to pinus sylvestris seedlings one month after emergence of seedlings. After one month of growth, a trichoderma virens fermentation broth is inoculated in the same manner. In 180 days, seedling growth is improved by 42.3%, the ground diameter is improved by 66.7%, peroxidase is improved by 268.3%, superoxide dismutase is improved by 66.18%, and no pest or disease occurs in the inoculated seedlings.
Description
Technical field
The present invention relates to a kind of method improving pinus sylvestris seedling resistance.
Background technology
Pinus sylvestris var. mongolica is northern main reproducting tree species, and its good drought-enduring resistance makes it in revegetation, restoration of the ecosystem and afforestation, all play important function.Seedling quality is the key of afforestation success or not.Introduced plant beneficial rhizosphere microorganisms (as VA Mycorrhizal Fungi and Trichoderma) is conducive to improving nursery soil environment, effectively can promote seedling growth, strengthen the generation of nursery stock resistance and pre-disease prevention.The resistance improving nursery stock with multiple-microorganism Mixed inoculation can obtain good effect, improves the survival rate of nursery stock.
Summary of the invention
The object of the invention is to overcome the weak point existed in above-mentioned technology, a kind of inoculation method improving the rhizosphere probio of Seedling Growth of Mongol Scotch Pine and resistance is provided.
In order to achieve the above object, the technical solution used in the present invention is:
(1) mycelium fermentation broth is cultivated: adopt potato dextrose medium, potato 180-250g, glucose 18-20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, water 1000mL, the medium made is loaded in the triangular flask of 500mL, liquid amount 200-300mL/500mL, be placed in 121 DEG C of high-pressure sterilizing pot sterilizing 20min, aseptically inoculate slippery jack and green trichoderma, inoculum concentration 10%-15%, postvaccinal triangular flask is placed in rotating speed 120-140r/min, the shaking table that temperature is 25 DEG C-27 DEG C is cultivated, fermentation number of days slippery jack 20d-30d, green trichoderma 3d-5d,
(2) microbial inoculum makes: slippery jack, green trichoderma zymotic fluid are aseptically pulverized, add sterile water and be made into zymotic fluid: sterile water concentration is 1: 5,
(3) seed treatment: will for planting experimentally son 0.5% potassium permanganate sterilization 30min, clear water rinses for several times, with the moisturizing of sterilizing wet gauze parcel, in climatic cabinate, vernalization at being placed in 25 DEG C, morning and evening every day sterile water respectively rinses once, until sprout,
(4) sterile soil preparation: by turfy soil, vermiculite and river sand by the proportions composite soil of 2: 1: 1, to put in high-temperature high-pressure sterilizing chamber sterilizing 2h at 121 DEG C, loads in diameter 15cm × high 13cm nutritive cube,
(5) sow: be sown in nutritive cube by the scotch pine seed through vernalization, every alms bowl 30 seeds, above cover the thick sterile soil of 2cm, water permeable after put into booth and cultivate, after coming up, final singling, to the strain of every alms bowl 15, carries out conventional dailyly to manage and protect,
(6) microbial inoculum inoculation: take to punch and fill with the mode of root inoculation, 30d inoculates emerging, every basin inoculation slippery jack microbial inoculum 40mL-50mL, after seedling growth 30d, inoculates green trichoderma microbial inoculum in the same way.
Advantage of the present invention is:
(1) Seedling Growth of Mongol Scotch Pine and degeneration-resistant level is improved;
(2) adopt punching to fill with the mode of root and spaced inoculations, to the pinus sylvestris seedling inoculation slippery jack zymotic fluid of emerging 1 month, grow after 1 month, adopt and inoculate green trichoderma zymotic fluid in the same way.Within 180 days, seedling-height growth improves 42.3%, and leading thread improves 66.7%, and peroxidase improves 268.3%, and superoxide dismutase improves 66.18%, the disease-free generation of inoculation nursery stock.
Depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center
Address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica
Preservation date: on June 4th, 2014
Deposit number: 9246 (N94) 9264 (M43)
Classification And Nomenclature: slippery jack, suillus luteus; Green trichoderma Trichodermavirens
Embodiment
Below embodiments of the invention are described in further detail.
Embodiment 1,
(1) mycelium fermentation broth is cultivated: the potato dextrose medium of employing, potato 180g, glucose 18g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, water 1000mL, loads in the triangular flask of 500mL by the medium made, liquid amount 200mL/500mL, be placed in 121 DEG C of high-pressure sterilizing pot sterilizing 20min, aseptically inoculate slippery jack and green trichoderma, inoculum concentration 10%, is placed in rotating speed 120r/min by postvaccinal triangular flask, the shaking table that temperature is 25 DEG C is cultivated, fermentation number of days slippery jack 20d, green trichoderma 3d
(2) microbial inoculum makes: slippery jack, green trichoderma zymotic fluid are aseptically pulverized, add sterile water and be made into zymotic fluid: sterile water concentration is 1: 5,
(3) seed treatment: will for planting experimentally son 0.5% potassium permanganate sterilization 30min, clear water rinses for several times, with the moisturizing of sterilizing wet gauze parcel, in climatic cabinate, vernalization at being placed in 25 DEG C, morning and evening every day sterile water respectively rinses once, until sprout,
(4) sterile soil preparation: by turfy soil, vermiculite and river sand by the proportions composite soil of 2: 1: 1, to put in high-temperature high-pressure sterilizing chamber sterilizing 2h at 121 DEG C, loads in diameter 15cm × high 13cm nutritive cube,
(5) sow: be sown in nutritive cube by the scotch pine seed through vernalization, every alms bowl 30 seeds, above cover the thick sterile soil of 2cm, water permeable after put into booth and cultivate, after coming up, final singling, to the strain of every alms bowl 15, carries out conventional dailyly to manage and protect,
(6) microbial inoculum inoculation: take to punch and fill with the mode of root inoculation, 30d inoculates emerging, every basin inoculation slippery jack microbial inoculum 40mL, after seedling growth 30d, inoculates green trichoderma microbial inoculum in the same way.
Embodiment 2,
(1) mycelium fermentation broth is cultivated: the potato dextrose medium of employing, potato 210g, glucose 19g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, water 1000mL, loads in the triangular flask of 500mL by the medium made, liquid amount 260mL/500mL, be placed in 121 DEG C of high-pressure sterilizing pot sterilizing 20min, aseptically inoculate slippery jack and green trichoderma, inoculum concentration 12%, is placed in rotating speed 130r/min by postvaccinal triangular flask, the shaking table that temperature is 26 DEG C is cultivated, fermentation number of days slippery jack 25d, green trichoderma 4d
(2) microbial inoculum makes: slippery jack, green trichoderma zymotic fluid are aseptically pulverized, add sterile water and be made into zymotic fluid: sterile water concentration is 1: 5,
(3) seed treatment: will for planting experimentally son 0.5% potassium permanganate sterilization 30min, clear water rinses for several times, with the moisturizing of sterilizing wet gauze parcel, in climatic cabinate, vernalization at being placed in 25 DEG C, morning and evening every day sterile water respectively rinses once, until sprout,
(4) sterile soil preparation: by turfy soil, vermiculite and river sand by the proportions composite soil of 2: 1: 1, to put in high-temperature high-pressure sterilizing chamber sterilizing 2h at 121 DEG C, loads in diameter 15cm × high 13cm nutritive cube,
(5) sow: be sown in nutritive cube by the scotch pine seed through vernalization, every alms bowl 30 seeds, above cover the thick sterile soil of 2cm, water permeable after put into booth and cultivate, after coming up, final singling, to the strain of every alms bowl 15, carries out conventional dailyly to manage and protect,
(6) microbial inoculum inoculation: take to punch and fill with the mode of root inoculation, 30d inoculates emerging, every basin inoculation slippery jack microbial inoculum 45mL, after seedling growth 30d, inoculates green trichoderma microbial inoculum in the same way.
Embodiment 3,
(1) mycelium fermentation broth is cultivated: the potato dextrose medium of employing, potato 250g, glucose 20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, water 1000mL, loads in the triangular flask of 500mL by the medium made, liquid amount 300mL/500mL, be placed in 121 DEG C of high-pressure sterilizing pot sterilizing 20min, aseptically inoculate slippery jack and green trichoderma, inoculum concentration 15%, is placed in rotating speed 140r/min by postvaccinal triangular flask, the shaking table that temperature is 27 DEG C is cultivated, fermentation number of days slippery jack 30d, green trichoderma 5d
(2) microbial inoculum makes: slippery jack, green trichoderma zymotic fluid are aseptically pulverized, add sterile water and be made into zymotic fluid: sterile water concentration is 1: 5,
(3) seed treatment: will for planting experimentally son 0.5% potassium permanganate sterilization 30min, clear water rinses for several times, with the moisturizing of sterilizing wet gauze parcel, in climatic cabinate, vernalization at being placed in 25 DEG C, morning and evening every day sterile water respectively rinses once, until sprout,
(4) sterile soil preparation: by turfy soil, vermiculite and river sand by the proportions composite soil of 2: 1: 1, to put in high-temperature high-pressure sterilizing chamber sterilizing 2h at 121 DEG C, loads in diameter 15cm × high 13cm nutritive cube,
(5) sow: be sown in nutritive cube by the scotch pine seed through vernalization, every alms bowl 30 seeds, above cover the thick sterile soil of 2cm, water permeable after put into booth and cultivate, after coming up, final singling, to the strain of every alms bowl 15, carries out conventional dailyly to manage and protect,
(6) microbial inoculum inoculation: take to punch and fill with the mode of root inoculation, 30d inoculates emerging, every basin inoculation slippery jack microbial inoculum 50mL, after seedling growth 30d, inoculates green trichoderma microbial inoculum in the same way.
Claims (1)
1. improve a method for pinus sylvestris seedling resistance, it is characterized in that:
(1) mycelium fermentation broth is cultivated: adopt potato dextrose medium, potato 180-250g, glucose 18-20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, water 1000mL, the medium made is loaded in the triangular flask of 500mL, liquid amount 200-300mL/500mL, be placed in 121 DEG C of high-pressure sterilizing pot sterilizing 20min, aseptically inoculate slippery jack and green trichoderma, inoculum concentration 10%-15%, postvaccinal triangular flask is placed in rotating speed 120-140r/min, the shaking table that temperature is 25 DEG C-27 DEG C is cultivated, fermentation number of days slippery jack 20d-30d, green trichoderma 3d-5d,
(2) microbial inoculum makes: slippery jack, green trichoderma zymotic fluid are aseptically pulverized, add sterile water and be made into zymotic fluid: sterile water concentration is 1: 5,
(3) seed treatment: will for planting experimentally son 0.5% potassium permanganate sterilization 30min, clear water rinses for several times, with the moisturizing of sterilizing wet gauze parcel, in climatic cabinate, vernalization at being placed in 25 DEG C, morning and evening every day sterile water respectively rinses once, until sprout,
(4) sterile soil preparation: by turfy soil, vermiculite and river sand by the proportions composite soil of 2: 1: 1, to put in high-temperature high-pressure sterilizing chamber sterilizing 2h at 121 DEG C, loads in diameter 15cm × high 13cm nutritive cube,
(5) sow: be sown in nutritive cube by the scotch pine seed through vernalization, every alms bowl 30 seeds, above cover the thick sterile soil of 2cm, water permeable after put into booth and cultivate, after coming up, final singling, to the strain of every alms bowl 15, carries out conventional dailyly to manage and protect,
(6) microbial inoculum inoculation: take to punch and fill with the mode of root inoculation, 30d inoculates emerging, every basin inoculation slippery jack microbial inoculum 40mL-50mL, after seedling growth 30d, inoculates green trichoderma microbial inoculum in the same way.
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| CN201410275883.7A CN104396707A (en) | 2014-06-19 | 2014-06-19 | Method for improving stress resistance of pinus sylvestris seedling |
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| CN201410275883.7A CN104396707A (en) | 2014-06-19 | 2014-06-19 | Method for improving stress resistance of pinus sylvestris seedling |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107896690A (en) * | 2017-10-18 | 2018-04-13 | 内蒙古和盛生态科技研究院有限公司 | A kind of nutrient solution for promoting the fast quick-recovery of pinus sylvestris var. mongolica tree vigo(u)r |
| CN108271623A (en) * | 2018-02-05 | 2018-07-13 | 中国林业科学研究院林业研究所 | A kind of culture medium, solid mycorhiza preparation and preparation method thereof |
| CN108477223A (en) * | 2018-05-07 | 2018-09-04 | 东北林业大学 | The preparation process of the antibacterial metabolite biocontrol agent aquas of green trichoderma M43 and its application |
| CN109769568A (en) * | 2019-03-11 | 2019-05-21 | 中国神华能源股份有限公司 | A kind of method of opencast coal mine dump restoration of the ecosystem |
| CN109879931A (en) * | 2018-05-23 | 2019-06-14 | 西北工业大学 | A method of utilizing crystallisation separating-purifying superoxide dismutase |
-
2014
- 2014-06-19 CN CN201410275883.7A patent/CN104396707A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107896690A (en) * | 2017-10-18 | 2018-04-13 | 内蒙古和盛生态科技研究院有限公司 | A kind of nutrient solution for promoting the fast quick-recovery of pinus sylvestris var. mongolica tree vigo(u)r |
| CN108271623A (en) * | 2018-02-05 | 2018-07-13 | 中国林业科学研究院林业研究所 | A kind of culture medium, solid mycorhiza preparation and preparation method thereof |
| CN108477223A (en) * | 2018-05-07 | 2018-09-04 | 东北林业大学 | The preparation process of the antibacterial metabolite biocontrol agent aquas of green trichoderma M43 and its application |
| CN109879931A (en) * | 2018-05-23 | 2019-06-14 | 西北工业大学 | A method of utilizing crystallisation separating-purifying superoxide dismutase |
| CN109769568A (en) * | 2019-03-11 | 2019-05-21 | 中国神华能源股份有限公司 | A kind of method of opencast coal mine dump restoration of the ecosystem |
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Application publication date: 20150311 |