CN104472060A - Method for increasing germination rate of flemingiaphilippinensis seeds - Google Patents
Method for increasing germination rate of flemingiaphilippinensis seeds Download PDFInfo
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- CN104472060A CN104472060A CN201410826882.7A CN201410826882A CN104472060A CN 104472060 A CN104472060 A CN 104472060A CN 201410826882 A CN201410826882 A CN 201410826882A CN 104472060 A CN104472060 A CN 104472060A
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Abstract
The invention relates to the field of planting of crop seeds, in particular to a method for increasing the germination rate of flemingiaphilippinensis seeds. The method is characterized by comprising steps as follows: 1) seed selection; 2) scratching treatment of seed coats; 3) disinfection; 4) seed soaking; 5) wet layer treatment; 6) seeding. With the adoption of the method, the water absorption and the air permeability of the flemingiaphilippinensis seeds are greatly enhanced after seed selection, seed coat scratching and seed soaking treatment, the seeds are promoted to germinate quickly, the germination rate is high, seedlings grow uniformly, the technical problem that the flemingiaphilippinensis seeds do not germinate or are low in germination rate in the prior art is solved, and the method can be widely applied to artificial cultivation of flemingiaphilippinensis and has the broad market prospect.
Description
Technical field
The present invention relates to crop seeds field of planting, particularly relate to a kind of method improving Flemingia philippinensis percentage of seedgermination.
Background technology
Flemingia philippinensis, also known as hanging hitching post, hangs Ma Dun, a root, rat tail, Chinese hydrangeavine root-bark etc.Upright or hang down loosely undershrub.Sprout triangular prism shape, close by taupe pubescence.Leaf tool finger-like 3 leaflet; Stipule wire lanceolar, is about 0.6-1cm, has longitudinal grin, by hair, and tip apicule, Su Cun.On the wilderness, level land being often born in height above sea level 50-300m or meadow, roadside, hillside.Because of its root hyoscine, dispel rheumatism, stimulate the circulation of the blood and cause the muscles and joints to relax, strengthen muscles and bones, the effect such as anti-inflammatory analgetic, there is good pharmacological action pursuing deeply by plantation family.But Flemingia philippinensis seed is waterproof, airtight, and germination rate is extremely low under field conditions (factors), germinate irregular, have a strong impact on the uniformity of this plant grown in field, give the problem that plantation family brings plantation difficult.So by processing Flemingia philippinensis seed, the germination rate improving seed seems most important in process of production.Method provided by the invention can solve waterproof, the air-locked problem of Flemingia philippinensis seed effectively, has the advantage that seed germination is fast, germination rate is high, growth of seedling is neat, among the artificial cultivation that can be widely used in Flemingia philippinensis.
Summary of the invention
In order to solve Problems existing in background technology, the invention provides a kind of method improving Flemingia philippinensis percentage of seedgermination, it is characterized in that, comprise the following steps:
1) choose seeds: results Flemingia philippinensis mature seed, through threshing, remove impurity, airing, it is for subsequent use that selected full, unabroken seed natural air drying is placed on 4 DEG C of Cord blood, at being prepended to 35 DEG C, dry 24h.Carry out the baked seed in the described time, can promote that seed physiology is ripe, promote seed metabolism, thus improve the survival rate of seed.
2) seed coat scratch process: seed is placed in pure white machine and carries out scratch process, the processing time is 90s, is abraded by seed coat, increases the ventilative of seed and water penetration, is beneficial to Seed imbibes and aerobic respiration.
3) sterilize: sterilize 30min in the clorox of 10%, more fully clean up with distilled water, sterilizing, prevents infection process.
4) soak seed: seed is soaked 6h in 40 DEG C of clear water, can seed coat be softened, impel Seed imbibes to expand, make the increased activity of enzyme, impel good the growing of embryo.
5) wet layer process: pretreated seed being placed in temperature is that the wet sand of 30 DEG C buries, from the 2nd day, observe seed whether have breach, every day observes once, carries out next step operation when the seed observing 40 ~ 50% splits and has radicle to expose; During process, every day supplements a small amount of moisture, makes sand bed absolute water content remain on 20%.
6) sow: germination is carried out to seed and cultivates or directly sow.Seed after disinfecting is placed in the culture dish being lined with filter paper and cultivates or directly sow, and the temperature of cultivation is 30 DEG C, and the ambient humidity of plantation controls 70%, controls the plantation temperature and humidity of seed well, improves survival rate and the germination rate of seed greatly.
Preferably, the method for described raising Flemingia philippinensis percentage of seedgermination, is characterized in that, selected maturation, full seed in described seed selection step, and use product bake out temperature to control at 35 DEG C, the water content of seed is no more than 10%.
Preferably, the method for described raising Flemingia philippinensis percentage of seedgermination, is characterized in that, in described seed coat scratch treatment step, seed is placed in pure white machine and carries out scratch process, the processing time is 90s, is abraded by seed coat.
Preferably, the method for described raising Flemingia philippinensis percentage of seedgermination, is characterized in that, sterilize seed in the clorox of 10% in described sterilisation step 30min, by seed at 40 DEG C of bubbly water 6h.
Preferably, the method for described raising Flemingia philippinensis percentage of seedgermination, is characterized in that, in described wet layer treatment step, pretreated seed being placed in temperature is that the wet sand of 30 DEG C buries, and sand bed absolute water content remains on 20%.
Beneficial effect of the present invention, adopt method provided by the invention by seed selection, seed coat scratch process and sterilization, seed soaking, Flemingia philippinensis Seed imbibes after wet layer process and gas permeability strengthen greatly, Seed imbibes expands, make the increased activity of enzyme, impel good the growing of embryo, then by sterilize and control well sowing temperature, humidity impels seed germination fast, germination rate is high, growth of seedling is neat, solve the Flemingia philippinensis seed existed in prior art waterproof, airtight, plant strain growth slowly and irregular technical problem of germinateing, in the middle of the artificial cultivation that can be widely used in Flemingia philippinensis, there are good market prospects.
Embodiment
Following examples further illustrate of the present invention, but absolutely not limit the scope of the present invention.Elaborate the present invention further referring to embodiment, but it will be appreciated by those skilled in the art that the present invention is not limited to the preparation method of these embodiments and use.And those skilled in the art can carry out equivalent replacement, combination, improvement to the present invention according to description of the invention or modify, and these all comprise within the scope of the invention.
Embodiment
Test establishes A to abrade process (s), B disinfecting time (min), C seed soaking time (h), D wet layer treatment temperature (DEG C) 4 factor, and each factor design 3 levels, specifically in table 1.
Table 1. tests process factor and level
| Level | A scratch process (s) | B disinfecting time (min) | C seed soaking time (h) | D wet layer treatment temperature (DEG C) |
| 1 | 0 | 0 | 6 | 25 |
| 2 | 90 | 15 | 12 | 30 |
| 3 | 180 | 30 | 18 | 35 |
Test adopts L
9(3
4) Orthogonal Experiment and Design, repeat for 2 times, overall design test is as table 2.
Table 2.L
9(3
4) orthogonal test designs table and result
Table 3. orthogonal design variance analysis table (random district group model):
| Source of variation | Sum of squares | Degree of freedom | All square | F value | Significance level |
| District's group | 0.0556 | 1 | 0.05556 | ||
| 1st row | 11045.3333 | 2 | 5522.66667 | 379.41985 | 0.00000 |
| 2nd row | 1156.0000 | 2 | 578.00000 | 39.70992 | 0.00007 |
| 3rd row | 664.3333 | 2 | 332.16667 | 22.82061 | 0.00049 |
| 4th row | 1520.3333 | 2 | 760.16667 | 52.22519 | 0.00003 |
| Error | 116.44444 | 8 | 14.55556 | ||
| Summation | 14502.50000 |
Significance of difference SSR inspection between each process of table 4.
Intuitive analysis and variance analysis is done by orthogonal experiment acquired results, table 3 shows, the factor order affecting Flemingia philippinensis seed sprouting is: scratch processing time (A) > wet layer treatment temperature (D) > disinfecting time (B) > seed soaking time (C).Shown by table 4 the results of analysis of variance, process 6 germination rates the highest, process 6 and process 4 without significant difference, but reach pole significant difference with other treatment combinations, the optimised process drawn thus is combined as (processing 6) A
2b
3c
1d
2.
As can be seen from statistical form, adopt method provided by the invention by basking seeds, seed coat scratch and seed soaking after Flemingia philippinensis Seed imbibes and gas permeability greatly strengthen, impel good the growing of embryo, then impelled by temperature, the humidity of sterilizing and control to sow that seed germination is fast, germination rate is high.Singly carry out basking seeds process, immersion treatment or both combine, Flemingia philippinensis seed germination is slow, and germinating time is of a specified duration, and germination rate is low and germinate irregular.
Although embodiment of the present invention are open as above, but it is not restricted to listed in specification and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the embodiment described.
Claims (6)
1. improve a method for Flemingia philippinensis percentage of seedgermination, it is characterized in that, comprise the following steps:
1) choose seeds: results Flemingia philippinensis mature seed, through threshing, remove impurity, airing, selected full unabroken seed.
2) seed coat scratch process: the seed after air-dry is placed in pure white machine and carries out scratch and process, the processing time is 90s.
3) sterilize: sterilize 30min in the clorox of 10%, more fully clean up with distilled water.
4) seed soaking: seed is put 40 DEG C of bubbly water 6h.
5) wet layer process: pretreated seed being placed in temperature is that the wet sand of 30 DEG C buries, from the 2nd day, observe seed whether have breach, every day observes once, carries out next step operation when the seed observing 40 ~ 50% splits and has radicle to expose; During process, every day supplements a small amount of moisture, makes sand bed absolute water content remain on about 20%.
6) carry out germination to seed cultivate or directly sow.
2. according to the method for the raising Flemingia philippinensis percentage of seedgermination described in claim 1, it is characterized in that, seed requires mature and plump, and the water content of seed is lower than 10%.
3. according to the method for the raising Flemingia philippinensis percentage of seedgermination described in claim 1, it is characterized in that, in described seed coat scratch treatment step, carry out seed coat scratch process 90s with pure white machine.
4. according to the method for the raising Flemingia philippinensis percentage of seedgermination described in claim 1, it is characterized in that, with the 30min that sterilizes in the clorox of 10% in described sterilisation step.
5. according to the method for the raising Flemingia philippinensis percentage of seedgermination described in claim 1, it is characterized in that, with 40 DEG C of water logging kind 6h in described seed soaking step.
6. according to the method for the raising Flemingia philippinensis percentage of seedgermination described in claim 1, it is characterized in that, bury with the wet sand of 30 DEG C in described wet layer treatment step, sand humidity is 20%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108718887A (en) * | 2018-06-06 | 2018-11-02 | 贺州学院 | A method of improving Flemingia philippinensis seed-setting rate and grain weight |
| CN110121977A (en) * | 2019-06-28 | 2019-08-16 | 广西壮族自治区药用植物园 | The method for abolishing Flemingia philippinensis seed dormancy |
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| CN101611678A (en) * | 2008-06-23 | 2009-12-30 | 株洲千金药业股份有限公司 | A kind of method for cultivating flemingia macrophylla seedlings |
| CN103766041A (en) * | 2014-01-22 | 2014-05-07 | 江西省鑫隆农业发展有限公司 | Germination accelerating method and planting method for flemingia philippinensis |
| CN104067820A (en) * | 2014-06-25 | 2014-10-01 | 江西省鑫隆农业发展有限公司 | Standard planting method of Philippine flemingia roots |
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2014
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Patent Citations (4)
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| CN101185414A (en) * | 2007-12-25 | 2008-05-28 | 中国科学院西双版纳热带植物园 | Artificial fecundation method for flemingia macrophylla |
| CN101611678A (en) * | 2008-06-23 | 2009-12-30 | 株洲千金药业股份有限公司 | A kind of method for cultivating flemingia macrophylla seedlings |
| CN103766041A (en) * | 2014-01-22 | 2014-05-07 | 江西省鑫隆农业发展有限公司 | Germination accelerating method and planting method for flemingia philippinensis |
| CN104067820A (en) * | 2014-06-25 | 2014-10-01 | 江西省鑫隆农业发展有限公司 | Standard planting method of Philippine flemingia roots |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108718887A (en) * | 2018-06-06 | 2018-11-02 | 贺州学院 | A method of improving Flemingia philippinensis seed-setting rate and grain weight |
| CN110121977A (en) * | 2019-06-28 | 2019-08-16 | 广西壮族自治区药用植物园 | The method for abolishing Flemingia philippinensis seed dormancy |
| CN114026990A (en) * | 2019-06-28 | 2022-02-11 | 广西壮族自治区药用植物园 | Grinding wheel machine, application of grinding wheel machine and method for breaking dormancy of flemingia sprains seeds |
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| CN104472060B (en) | 2016-08-17 |
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