CN108635618B - Preparation method of medical adhesive with healing promoting and repairing functions - Google Patents

Abstract

The invention discloses a preparation method of medical adhesive with healing promoting and repairing functions, which is characterized by comprising the following components in parts by weight: 20-25 parts of a-n-butyl cyanoacrylate, 25-35 parts of a-isooctyl cyanoacrylate, 2-6 parts of styrene thermoplastic elastomer microspheres, 25-37 parts of efficient healing promoting and repairing components, 2-4 parts of sodium alginate, 3-6 parts of sodium hyaluronate, 8-10 parts of allantoin, 1 part of hydrolyzed wheat protein, 1 part of stabilizer, 220 parts of compound fruit oil component 150 and 200 parts of sterile water. The medical adhesive of the invention particularly selects various biological extracts with regeneration function, adds three components for promoting healing and repairing to promote healing and improve repairing effect, has high bonding strength and good biocompatibility, and meets the clinical application requirement on the medical adhesive.

Description

Preparation method of medical adhesive with healing promoting and repairing functions

Technical Field

The invention belongs to the technical field of medical materials, and particularly relates to medical glue with healing promoting and repairing functions and a preparation method thereof.

Background

With medical advances, clinical requirements for surgical techniques and the effects of auxiliary materials are becoming higher and higher. Modern medicine demands that the pain of patients be minimized and the repair of scars after surgery be emphasized, especially in the medical cosmetic and plastic industry, the postoperative recovery and beauty are important. The medical adhesive is a branch of medical macromolecules, takes biological tissues as an adhesive object, and can replace partial suture and ligation operations by applying an adhesive technology to most tissues and organs of a human body in the treatment process without anesthesia; the wound is automatically dropped after healing. Compared with the traditional method, the use of the medical adhesive can effectively shorten the operation time, reduce the pain of the patient, and simultaneously realize the quick repair of the wound and ensure the beauty.

At present, the main research direction of medical adhesives is to improve the bonding strength of various adhesives, however, biomedical materials applied in the current surgical operation rarely have the efficient effects of promoting healing and repairing scars, the remaining of ugly scars brings huge mental and psychological pressure to patients, and the function of promoting healing and repairing medical materials is an effective method for solving the problem. The generation of formaldehyde in the storage and use processes of cyanoacrylate medical adhesive has side effects on organism tissues, and the damage to organisms is increased along with the increase of the concentration of the formaldehyde, thereby seriously influencing the effect and the application of the medical adhesive.

Disclosure of Invention

Based on the prior art, the invention aims to provide the medical adhesive which combines a plurality of effective components to greatly improve the healing rate, repair and reduce scars and effectively inhibit formaldehyde generation and has the functions of promoting healing and repairing, and the preparation method thereof.

The purpose of the invention is realized by the following technical scheme:

the medical adhesive with the functions of promoting healing and repairing is characterized by comprising the following components in parts by weight: 20-25 parts of a-n-butyl cyanoacrylate, 25-35 parts of a-isooctyl cyanoacrylate, 2-6 parts of styrene thermoplastic elastomer microspheres, 25-37 parts of efficient healing promoting and repairing components, 2-4 parts of sodium alginate, 3-6 parts of sodium hyaluronate, 8-10 parts of allantoin, 1 part of hydrolyzed wheat protein, 1 part of stabilizer, 220 parts of compound fruit oil component 150 and 200 parts of sterile water;

the high-efficiency healing promoting and repairing component comprises 6 parts of specific healing promoting and repairing component, 12-24 parts of natural healing promoting and repairing component and 6-12 parts of traditional Chinese medicine healing promoting and repairing component;

the components for promoting healing and repairing specially comprise 2 parts of jellyfish extract, 2 parts of starfish extract, 1 part of gecko extract, 1 part of salamander essence, 4 parts of octopus polysaccharide and 4 parts of fish skin collagen in parts by weight;

the natural healing promoting and repairing component comprises 2 parts of tricholoma matsutake extract, 2 parts of lycium ruthenicum extract, 3 parts of cocoa seed extract and 5 parts of kiwi fruit extract in parts by weight;

the traditional Chinese medicine components for promoting healing and repairing comprise, by weight, 0.5 part of ganoderma sinensis extract, 2 parts of earthworm extract, 2 parts of turmeric extract and 1.5 parts of cordyceps sinensis extract;

the composite fruit oil component consists of aloe oil, rose hip oil, evening primrose oil and Pu' er tea fruit oil according to the weight fraction of 5:3:2: 1.

Further, the stabilizer is polyanionic cellulose and poloxamer according to the weight part ratio of 5: 1, combining the components; the starfishes in the starfish extract consist of asterias rollestoni, asterias amurensis and the starfish in a weight ratio of 1:1: 1; the particle size range of the styrene thermoplastic elastomer microspheres is 50nm-3 mu m, and the particle size distribution is less than 0.5.

Further, the specific extraction steps of the jellyfish and starfish extracts are as follows: respectively taking freshly collected jellyfish and starfish as raw materials, cleaning the raw materials, shearing the raw materials, adding 95% ethanol solution which is 6 times of the weight of the raw materials according to the weight-volume ratio, and performing reflux extraction for 3 times, 6 hours each time; mixing extractive solutions, and recovering solvent to obtain jellyfish extract and starfish extract.

Further, the preparation method of the cocoa seed extract specifically comprises the following steps: drying and pulverizing cacao seeds, sieving with 100 mesh sieve to obtain powder of cacao seeds, adding 95% ethanol solution, and ultrasonic extracting at 45 deg.C and 200W for 35min to obtain extractive solution; filtering the extractive solution, and concentrating under reduced pressure to obtain slurry; the slurry was mixed at a ratio of 1: dissolving the extract in deionized water at a volume ratio of 15, stirring, shaking, and sterilizing with microporous membrane to obtain cacao seed extract.

Further, the preparation method of the lycium ruthenicum extract comprises the following steps: washing away silt on the surface of the lycium ruthenicum by using running water, introducing an ozone generator into sterile water to wash the lycium ruthenicum again, crushing pulp into pulp, adding deionized water according to the volume of 30% of the pulp, adding 2% of pectinase at the same time, uniformly stirring, and standing for 50min at 33 ℃; adding the supernatant into 95% ethanol solution, ultrasonic extracting at 40 deg.C and 200W ultrasonic power for 20min, and centrifuging at 4000rpm/min for 10 min; after concentrating the supernatant from the centrifugation to a semi-fluid slurry, the slurry was mixed at a ratio of 1: dissolving the extract in deionized water at a volume ratio of 20, stirring, shaking, and sterilizing with microporous membrane to obtain Lycium ruthenicum Murr extract.

Further, the ganoderma sinensis extract, the earthworm extract, the turmeric extract and the cordyceps sinensis extract are extracted by the following specific steps: respectively pulverizing dried Ganoderma, Lumbricus, Curcuma rhizome and Cordyceps, sieving with 200 mesh sieve, adding 90% ethanol solution 20 times the weight of the powder, soaking for 60 hr, filtering, and centrifuging at 3000 rpm/min for 10 min; collecting supernatant, concentrating by rotary evaporation to concentration of 1g/ml, and sterilizing the concentrated solution with microporous membrane filter to obtain Ganoderma extract, Lumbricus extract, Curcuma rhizome extract and Cordyceps extract.

A preparation method of medical glue with the functions of promoting healing and repairing is characterized by comprising the following steps:

step one, adding sodium alginate, sodium hyaluronate, allantoin, hydrolyzed wheat protein and a stabilizer into sterile water according to a part ratio, heating to 75 ℃, stirring for dissolving for 150min, and cooling to room temperature to obtain an aqueous solution A;

step two, putting the a-n-butyl cyanoacrylate, the a-isooctyl cyanoacrylate and the styrene thermoplastic elastomer microspheres into the composite fruit oil according to the parts ratio, heating to 70 ℃, stirring for 80min, and cooling to room temperature to obtain a composite fruit oil solution B;

step three, uniformly mixing the aqueous solution A and the composite fruit oil solution B, adding efficient healing promoting and repairing components according to the parts ratio, and stirring for 3.5 hours;

and step four, filtering and sterilizing the product obtained in the step three, subpackaging, sealing and packaging to obtain the medical adhesive with the healing promoting and repairing functions.

The medical alpha-cyanoacrylate glue has developed fast because of its unique advantages of single component, no solvent, no need of added catalyst, etc. and may be adhered fast at normal temperature. Moreover, the adhesive has the characteristics of wide adhesive range, high strength and instant curing, and more importantly, the medical adhesive has no toxic or side effect on organisms such as skin and the like. The particularity of the chemical structure determines the excellent property of the alpha-cyanoacrylate in adhesion, and the alpha-carbon atom in the molecular structure CNCH2CCOOR of the alpha-cyanoacrylate compound has extremely strong ability of attracting electrons, so that the chemical reaction can be immediately carried out when the alpha-cyanoacrylate compound contacts with a very small amount of anions. The basic substance of the organism, namely protein amino acid chains, are provided with a large number of electronegative groups-NH 2 and-COOH, and when the basic substance of the organism is contacted with alpha-cyanoacrylate, the reaction can be rapidly carried out, and the principle also enables the medical adhesive to be widely applied to clinic. The effect of α -cyanoacrylates on tissue is strongly related to the type and size of the alkyl group. Generally, the longer the ester carbon chain, the slower the polymerization reaction rate, the less irritation to the tissue, and the better the flexibility of the adhesive layer. The invention selects the alpha-n-butyl cyanoacrylate and the alpha-isooctyl cyanoacrylate as the main raw materials in the system, and the alpha-n-butyl cyanoacrylate and the isooctyl cyanoacrylate have good biocompatibility and excellent healing promotion and repair performance. The needle suture operation is replaced, so that no trace is left after the skin incision is anastomosed.

The styrene thermoplastic elastomer microspheres have good biocompatibility, blood compatibility, light transmittance, air permeability and the like, have the characteristics of excellent bonding strength and durable bonding when used in blood and tissue fluid environments, and have unique effects in the aspect of tissue repair. The polymer microspheres have the property of an adhesive, can be used for repairing tissues and stopping bleeding, and can also play a role of a dispersing agent in a system.

Allantoin, also known as 5-ureylethanolamide, ureidoacetolactam, ureidohydantoin, uretidione, is a hydantoin derivative. At present, the annual production capacity of allantoin in the world is about 5 million tons, the market demand is mainly three fields of medicine, cosmetics and agriculture, and the traditional process for extracting natural allantoin from natural animals and plants is low in content, complex in process and high in cost, so that the allantoin cannot be applied in a large scale. In medicine, allantoin has physiological functions of promoting cell growth, accelerating wound healing, softening keratin and the like, and is a good healing agent and an anti-ulcer agent for skin wounds. In addition, allantoin can be used for alleviating and treating xerosis cutis and skin ulcer, and has good therapeutic effect on inflammation. The allantoin is an amphoteric compound, can be combined with various substances to form a double salt, has the effects of protecting from light, sterilizing, preventing corrosion, relieving pain and resisting oxidation, can keep the skin moisture, and is more moist and soft.

The medical material prepared by taking alginate as a raw material has good affinity with wounds, has the characteristic of rapid hemostasis, can be used for repairing tissues and organs of a human body and has the property of promoting healing. The chemical molecular formula of sodium alginate is (C6H706Na) n, and the sodium alginate is a natural polysaccharide carbohydrate, and has the physicochemical properties that: the product is tasteless white or light yellow amorphous powder, is easily soluble in water, has strong hygroscopicity, is insoluble in organic solvents such as alcohol and chloroform, and has the characteristics of bioadhesion, biocompatibility, biodegradability and the like. Sodium alginate is used as a base material of drugs in the fields of medicine, biotechnology, and the like. Sodium alginate has been received in the United states pharmacopoeia as early as 1938. Alginic acid was incorporated in the british pharmacopoeia in 1963. Alginic acid is insoluble in water but swells when placed in water. Therefore, sodium alginate is used as a binding agent of tablets in the prior art, has unique advantages in thickening, and can prevent the viscosity reduction phenomenon in the product disinfection process due to good fluidity of sodium alginate. Meanwhile, sodium alginate can also be used as a stabilizer in the system, so that the storage and transportation of the product are more convenient.

Matsutake, a scientific name of Tricholoma matsutake, also called matsutake, belongs to the secondary protection plant of China. The matsutake is widely distributed in northeast and southwest areas of China, particularly the matsutake Singer in Sichuan, Yunnan and Jilin is the most popular name, wherein the matsutake Singer in Yajiang in Sichuan is popular at home and abroad due to high yield, large size, fine meat quality, good color and good taste, the collection quantity of the matsutake Singer in Yajiang is up to over 1000 tons every year, and the matsutake Singer is purchased from Shanwang to Yajiang in China, such as Japan, Korea and other countries. The matsutake which is a rare plateau wild rare fungus is rich in nutrition, contains various proteins, amino acids, polysaccharides, vitamins and mineral substances, has the effects of relieving pain and building the body, and can play a strong role in quickly supplying nutrition to a wound surface by the special polysaccharides, vitamins and mineral substances in the components.

The Lycium ruthenicum Murr extract is water extract of Lycium ruthenicum Murr fruit powder. The lycium ruthenicum fruit is rich in protein, amino acid, flavone, procyanidine, fat, polysaccharide, vitamin E and other components. The protein is a structural substance of human skin, provides nutrition to the skin, can repair damaged cells and tissues in time with high quality, and enables the skin to be glossy and elastic. The lycium ruthenicum fruit contains 17 amino acids except tryptophan, and the amino acid is one of the most basic substances which form biological protein and are closely related to the vital activities, and can provide nutrition, promote growth and maintain normal metabolism. The Lycium ruthenicum Murr pigment is anthocyanin and flavonoid, has oxidation resistance obviously higher than that of vitamin E, and has stabilizing effect on visible light and ultraviolet. The oxidation resistance of flavone is mainly shown in the aspects of scavenging free radicals, resisting lipidosome peroxidation and the like. The lycium ruthenicum fruit is rich in protein, amino acid, procyanidine, polysaccharide, vitamin E and other components, and can provide strong support for wound healing, high-quality recovery and beauty maintenance.

The cocoa seed extract, which is rich in cocoa peptides, saccharides and polyphenols, is highly effective as an antioxidant, and helps to protect the skin from oxidative stress due to blue light rays and photodamage, and is a supplementary measure of UV and IR protection, which helps to retain the youthful appearance of the skin. The saccharide and polyphenol components in the cacao seed extract can effectively slow down scar formation and assist the skin to keep beautiful.

The kiwi fruit contains abundant vitamin C, A, E, potassium, magnesium, cellulose, and other less common nutrients of fruits, such as folic acid, carotene, calcium, lutein, amino acids, and natural inositol. The vitamin C can strengthen the immune system, promote wound healing and absorb iron; inositol and amino acid can supplement consumed nutrition; the perfect proportion of low sodium and high potassium can supplement the physical strength lost by the patients who undergo the operation. One of the folic acid (folic acid) vitamin B complexes, which corresponds to pteroylglutamic acid (PGA), is purified from spinach leaves by michael (h.k. mitchell, 1941) and is thus named folic acid. Has effects in promoting maturation of immature cells in bone marrow, and can cause megaloblastic anemia and leukopenia due to folic acid deficiency in human. Folic acid is a substance necessary for human body to utilize sugar and amino acids, and is a substance necessary for growth and reproduction of body cells. Folic acid functions in vivo in the form of tetrahydrofolic acid, which is involved in the synthesis and conversion of purine and pyrimidine nucleotides in vivo. Folic acid plays an important role in the production of nucleic acids (ribonucleic acid, deoxyribonucleic acid). Folic acid, which helps protein metabolism and promotes the generation and maturation of erythrocytes together with vitamin B12, is an essential substance for the production of erythrocytes. Folic acid also functions as a proliferation-promoting factor for Lactobacillus casei (Lactobacillus casei) and other microorganisms. Therefore, the kiwi fruit extract can more effectively help and promote the healing of wounds and the high-speed repair, and plays a significant role in the components of the invention.

Starfish (starfish) was originally recorded in "Chinese medicinal animal journal" and is an animal of the Asteriodea (Asterioidea) of Echinodermata (Echinodermata). The variety is various, the resource is very rich, 5 meshes exist, nearly 1500 meshes exist, about 100 varieties exist in China, and the distribution is realized in deep water between intertidal zone and 600 m. The external form of the medicine is three wrists, four wrists, more than ten wrists and up to fifty wrists. The starfish is marine benthonic animal, is violently and completely murder, and specially eats clams, oysters, scallops, abalones, sea cucumbers and the like. The starfish can perform reproduction by breaking, and has strong reproductive capacity and regeneration capacity. Starfish is salty in taste and neutral in nature. It enters heart, stomach and large intestine meridians. Has effects in regulating stomach function, relieving pain, relieving hyperacidity, relieving diarrhea, and tranquilizing mind. Modern pharmacological experiments prove that the spina and gonad parts of the Chinese medicinal composition have good medicinal values, such as tumor resistance, virus resistance, bacteriostasis, inflammation resistance, ulcer resistance and the like. The basic structure of starfish consists of thorns, bone shells and internal organs. Its main components include sterol, glucoside, alkaloid, small amount of anthraquinone, lipid, polysaccharide, vitamins, protein, polypeptide and amino acids and terpenes, etc. The starfish saponin is the main source of the reproductive capacity and the regeneration capacity of the starfish saponin, most of the starfish saponin is combined with glycoprotein and lipoprotein, only a small part of the starfish saponin exists in a free state, and the saponin in the starfish belongs to steroid saponin which has the function of regulating reproduction and development, so that the starfish extract has good capacities of promoting healing, promoting reproduction and promoting cell proliferation on damaged wound surfaces, damaged tissues and the like. Starfish has a strong regenerative capacity and can quickly grow new parts at the wound site. The characteristic of starfish of quick wound repair has obvious curative effect on wound healing and broad-spectrum antibacterial performance.

Gekko Swinhonis is a generic name of all distinct scorpions of the family Gekkonidae (Sauria) of the order Vigores, commonly known as Tetrakistrodon acutus, eave snake, Gekko Swinhonis, Scorpion tiger, creeping gecko, eave dragon, Bagecko or Dalong dragon, and contains about 80 species of 750 species, wherein the Gekko Swinhonis belongs to about 20 species, 8 species are produced in China, and are mainly distributed in southwest and south regions of the Yangtze river basin in China. People have conducted intensive research on the medicinal value of the gecko in our country. The most of the drug effect of gecko is in the tail, so the tail of gecko is usually cut off to be used as drug, and the gecko is cut after regenerating a new tail, which has strong regeneration function. The Gekko Swinhonis contains various active ingredients including wasp venom analogue, protein, vitamins, histamine nucleoside, amino acids, etc., and active substances including protein and peptide mixture, adenosine, sulfated polysaccharide protein, etc. As one-door skill of geckos, tail-breaking escape has been an effective way to self-defend, and is called "self-interception" or "disabled self-defense" in biology. Glia growth factor-2 released by the nerve ending of the body after the tail of the gecko is cut off, and substance P and transferrin are factors for promoting the survival and proliferation of bud-based cells; growth factors, such as platelet-derived factor, fibroblast growth factor-2 (FGF-2), Epidermal Growth Factor (EGF), etc., cause the cell to re-enter the cell cycle. Therefore, the gecko extract can effectively improve and stimulate the proliferation and regeneration capacity of body cells. The polysaccharide proteoglycan moiety is a glycosaminoglycan, also known as a hexosamine polysaccharide, produced by fibroblasts and mainly classified into two types, sulfated and non-sulfated. The former group mainly comprises chondroitin sulfate, keratan sulfate, heparin sulfate and the like; the latter is hyaluronic acid, which is a long-chain macromolecule that is convoluted into a meander, and forms the backbone of the protein-polysaccharide complex, while other glycosaminoglycans bind to proteins to form proteoglycan subunits, which in turn are linked to the hyaluronic acid long-chain molecules by binding proteins to form proteoglycan polymers. A large amount of proteoglycan polymer forms a molecular sieve with a plurality of tiny gaps, so that the substrate becomes a barrier for limiting the growth of bacteria and has good antibacterial effect.

Jellyfish belongs to coelenteras of coelenterates and is divided into Jellyfish of the california class and Jellyfish of the water daughter-in-law class. Jellyfishes are distributed in temperate zone, subtropical zone and tropical sea area, and are widely distributed in coastal sea areas such as Shandong, Zhejiang, Fujian and Guangdong in China. The jellyfish consists of an umbrella part, a mouth and wrist part, an accessory and the like, contains abundant chemical substances such as protein, fat, carbohydrate, vitamin, nicotinic acid, phosphorus, iodine, iron and the like, and has good nutritional value and medicinal value. The record of "Bo Zhi" of the Hua jin Dynasty is that people have already begun to eat in the jin Dynasty. In medicine, it has the functions of clearing away heat and toxic material, softening hard mass and dissipating stagnation and lowering blood pressure, and may be used in treating chronic tracheitis, dermatitis and other diseases. Although amputated limb regeneration is a dream for mankind, it is a life-saving instinct for jellyfishes, and animals of the jellyfish category all have extremely strong regeneration capability. Jellyfish contains abundant nutrient substances, and scientists also extract vascular endothelial growth factor, antibacterial peptide, lectin and the like. The growth of the vascular endothelium can effectively stimulate cell proliferation and migration, participate in angiogenesis and permeability regulation, have good effects on healing of injured parts and tissue regeneration, and have high-efficiency antibacterial capacity for antibacterial peptides, agglutinin and the like, thereby playing an important auxiliary antibacterial role in the wound healing process. The jellyfish adopted by the invention is white cyanea nozakii.

The salamander is an amphibian with tails, the body shape is similar to that of lizard, but the body surface has no scales. The salamander has effects of eliminating dampness, relieving itching, relieving pain, clearing heat and detoxicating, and can be used for treating skin prurigo, burn, scald, etc. The adult can regenerate not only the complete tail and limbs, but also a plurality of tissues such as spinal cord, upper and lower collar bones, crystalline lens, retina and the like. The salamander contains the protein of multiple functional nature in vivo, for example salamander preceding gradient protein, salamander keratin, salamander surface protein PROD1, bone morphogenetic protein etc. still has a large amount of fibroblast growth factors, plays the effect of induction and promotion cell division proliferation simultaneously to the healing and the restoration of effectual promotion woundplast wound. Salamander essence consists of activated cells and activated cell decomposition products extracted from the body of salamander and rich in induced programming instructions. Because salamander element can promote any position of salamander body to regenerate to can provide directive property recoding procedure for the regeneration of wound, delay the sending of histocyte self healing instruction, strive for the time for sending of histocyte regeneration instruction, thereby promote the wound and regenerate before the scabbing, promote the perfect restoration of wound.

Collagen is a protein composed of specific amino acids. It is not only the most frequently occurring protein in animals and humans, but also the most important functional component of connective and supportive tissue within cells. Fish belongs to the subphylum vertebrate of the phylum chordata, is an aquatic cold blood vertebrate, breathes with gills, and has jaws and fins. Can be divided into two main groups: cartilaginous fish and teleostean. At present, over 2.6 thousands of fish species are named globally, and nearly 3 thousands of fish species exist in China, wherein about 1000 species of freshwater fish exist. The human body absorption rate of the fish protein reaches 87-98%, and the fish protein is rich in vitamin A, D and trace elements, and has very high nutritional value. Take freshwater fish as an example, 58-70 g of freshwater fish can be eaten, and 350-480 kilojoules of energy can be used. Per 100 grams of edible portion: 70-78 g of water, 0-2.4 g of carbohydrate, 15-24 g of protein, 1.8-3.2 g of fat and 0.6-1.8 g of ash, and the milk contains rich mineral substances, vitamins and trace elements. The fish is not only a dietary food, but also a good food therapy food, in particular to fish skin, and the medicinal efficacy of the fish skin is recorded in the drug books of the south and the Song in China. The theory of traditional Chinese medicine considers that: the fish skin is sweet, salty and neutral in flavor, and has nourishing effect. In the aspect of food therapy, the fish skin contains abundant proteins and a plurality of trace elements, the proteins are mainly macromolecular collagen and mucopolysaccharide components, and also contain abundant amino acids, which are all necessary components for protecting and repairing the skin and play an extremely important role in the process of repairing, healing and repairing the skin.

The active ingredients of Cordyceps sinensis extract include cordycepin, protein, amino acid, sterol, mannitol, alkaloid, polysaccharide and mineral; cordycepin has broad-spectrum antibacterial effect, and can inhibit growth of pathogenic bacteria such as streptococcus, Bacillus rhinoceros, Bacillus anthracis, Swine hemorrhagic septicemia bacillus, and staphylococcus. In addition, cordycepin also has inhibitory effect on pathogenic fungi of skin such as Trichophyton gypseum, Trichophyton lanosum and Trichophyton mentagrophytes, and Bacillus subtilis. Sugar proves that cordycepin has very strong antifungal activity, and cordycepin also has strong antiviral activity. The cordyceps sinensis is mild in medicine property, warm but not dry, and can tonify but not lead to stagnation, so that the cordyceps sinensis can tonify deficiency and strengthen the body, can treat diseases and prolong the life, is more stable in medicine property than ginseng and pilose antler, is not limited by physique, diseases, seasons and ages, and can be used for improving immunity and disease resistance. Meanwhile, cordyceps polysaccharide, superoxide dismutase SOD, 18 amino acids, vitamin VA, VB1, VB2 and trace elements such as calcium, iron, phosphorus, selenium and the like in the cordyceps sinensis extract can greatly improve the speed of recovery and healing of wounds.

Ganoderma sinensis is an important member of Ganoderma lucidum, and the Ganoderma sinensis is mainly distributed in subtropical and tropical regions of China and grows on broad-leaved tree aspergillosis, rotten wood and forest land. According to the records in the compendium of materia Medica: the purple ganoderma is a woody ganoderma, has sweet and warm smell and is non-toxic; it is mainly used for treating deafness, benefiting joint, protecting spirit, benefiting essence and qi, strengthening bones and muscles, good color, long-term taking, weight reduction, aging resistance and prolonging life (Li Shizhen 1978). Ganoderma sinense (Ganoderma sinense) is fruiting body of Ganoderma sinense (Ganoderma sinense) Zhao, Xu et Zhang of Polyporaceae. Scientific research shows that the pharmacological components of ganoderma lucidum are very rich, wherein the effective components can be divided into ten major groups, including ganoderma lucidum polysaccharide, ganoderma lucidum polypeptide, triterpenes, 16 amino acids (including seven essential amino acids for human bodies), proteins, steroids, mannitol, coumarins, alkaloids, organic acids (mainly including fumaric acid), and trace elements of Ge, P, Fe, Ca, Mn, Zn and the like. Ganoderma has bidirectional regulating effect on human body. In addition, most components in the ganoderma lucidum have the effect of caring the skin, which is different from the effect of treating certain diseases by common medicines, is also different from the effect of supplementing and strengthening the deficiency of certain nutrient in common nutritional health-care foods, and can regulate the function balance of the human body in two directions on the whole, mobilize the internal vitality of the body and regulate the metabolism function of the human body. It can also promote the synthesis of nucleic acid and protein: the polysaccharide compound contained in Ganoderma sinensis can promote synthesis of human protein and improve hemopoiesis function, thereby promoting healing and repair of diseases, pain parts and wound, and making organism reach a new balance state. The polysaccharide peptide specially contained in the ganoderma sinensis can effectively eliminate the capability of free radicals of a human body, reduce the production of lipofuscin in the heart and the brain, improve the activity and the metabolic capability of cells, delay the cell aging and keep skin cells tight, tight and bright, and is a top-quality product for repairing the skin.

Lumbricus, Li Shizhen, is called as the action of clearing and activating the channels and collaterals, promoting blood circulation and removing blood stasis in Ben Cao gang mu. Lumbricus is salty and cold in nature, and has effects of clearing heat, arresting convulsion, relieving asthma, dredging collaterals, and promoting blood circulation. Lumbricus can reduce exudation, shorten inflammation period, and promote wound healing. Meanwhile, the earthworm extract has the plasminogen activation effect and further has the promotion effect on wound healing. The earthworm has the functions of detoxifying, activating blood circulation to dissipate blood stasis, promoting granulation and relieving pain and inhibiting bacteria, contains various active components, such as earthworm extract, earthworm alkali, purine, choline, vitamins, amino acid and the like, has better fibrinolysis and anticoagulation effects, can increase the activity of plasminogen activator, thereby promoting the healing of wound surfaces more efficiently while relieving pain and inhibiting bacteria.

Curcuma xanthorrhiza of Zingiberaceae, a perennial herb of the genus Curcuma, is also known as Curcuma longa L (Latin's name: turmeric root-tuber, Baodingxiang, Chinese ephedra, ginger, etc. The main components of the medicine comprise curcumin, turmerone, aromatic-turmerone, curcumene, aromatic-curcumene, sterols, fatty acid, metal elements such as potassium, sodium, magnesium, calcium, manganese, iron, copper, zinc and the like. The curcumin has strong anti-inflammatory, antibacterial and antioxidant effects, and can be combined with polysaccharide substances in fish skin collagen, Cordyceps sinensis extract, octopus polysaccharide, Gekko Swinhonis extract and starfish extract, and cooperate with the polysaccharide substances to effectively heal wound surfaces.

Wheat is an important agricultural product and mainly planted in the middle and north areas of China, and for a long time, the wheat is mainly used for flour production. Protein is the second largest component in wheat flour, hydrolyzed wheat protein: the major amino acids of wheat, gliadin (gl5ad55) and glutenin, are rich in cystine (cyst55e), a sulfur-containing amino acid. It has effects of keeping moisture, resisting oxidation, softening and refining skin, moistening skin, and improving skin condition. Meanwhile, hydrolyzed wheat protein provides essential amino acid for the anabolism of the organism to maintain the balance of nitrogen in the body. Particularly useful for anti-inflammatory and post-surgical protein supplementation. That is, hydrolyzed wheat protein can simultaneously supplement protein nutrition, help skin repair and resist inflammation.

The sodium hyaluronate can improve the physiological condition of skin, provide excellent external environment for the synthesis of dermal collagen and elastic fiber, enhance the supply of nutrient substances and play a role in skin care. The hyaluronic acid can prevent the generation of some enzymes in cells, reduce the formation of free radicals, play an important role in preventing the free radicals from damaging cell structures, generating lipid peroxidation, causing body aging and the like, provide a proper place for cell proliferation and differentiation, directly promote cell growth, differentiation, reconstruction, repair and the like, achieve the effects of moistening and diminishing inflammation and have an excellent repair effect on damaged skin.

Aloe is a fleshy herbaceous plant of the family Liliaceae, native to Africa. The aloe introduced into China is mainly aloe vera, aloe arborescens and aloe ferox in the United states, and unique aloe Chinese is formed after many years of cultivation and planting. Aloe is rich in polysaccharide, protein, amino acids, vitamins, active enzyme and various trace elements. The typical component of the aloe is aloe anthraquinone which contains various bioactive substances and is used as a natural medicine for sterilizing, resisting inflammation, beautifying, protecting hair and treating skin diseases in folk. The properties of the medicine are that they enter liver meridian, heart meridian, stomach meridian and large intestine meridian. The main effects of aloe are as follows: sterilization; anti-inflammatory effects; moistening and beautifying effects; invigorating stomach and promoting defecation; strengthening heart and promoting blood circulation; immune and anti-tumor effects; detoxification function; anti-aging effect; relieving pain and tranquilizing effect. The aloe leaf is leaf and mainly contains anthraquinone compounds such as aloin and aloe-emodin. Anthraquinone compounds are the most important part of the organic active components of aloe, and include aloe-emodin glycoside (Aloin), also called Aloin, aloe-emodin (Aloeemodin). Wherein aloin is one of the most basic components, and the average content of aloin in Chinese aloe is shown by data: 335.28 mg/g. Reported by Dai et al: the Chinese aloe leaf polysaccharide comprises mannose, galactose, glucose, xylose, arabinose and rhamnose, and the total content is 3.45%. The relative contents of mannose are 60.73%, galactose 16.42%, glucose 9.16%, xylose 3.83%, arabinose 5.45% and rhamnose 4.42%. The total protein content is more than 9.50%.

Aloe oil has special health promotion effect on skin that other oils and fats can not replace, is suitable for various skin types, and is especially beneficial for oily skin and skin with inflammation. The aloe vera contains pure oily aloe vera gel which can provide deeper moistening and nutrition for injured skin and skin losing moisture, quickly repair damaged cell tissues, promote cell regeneration and quickly restore the health of the skin.

The rose hip oil deeply moistens the skin, provides nutrients, enables the skin to be soft, tender and smooth, prevents ultraviolet rays from damaging the skin, and whitens the skin; increasing skin tissue activity, keeping skin elasticity, recovering natural luster of dry cracked skin, and improving and eliminating new and old scar after wound, sunburn, burn scar, and scar after operation. The rose hip oil can improve the skin regeneration and renewal capacity by promoting the activity of epidermal cells, thereby promoting the regeneration of new skin, and can restore the optimal balance between fatty acid and water due to the rich natural vitamin C, thereby playing an effective role in moistening and maintaining the skin.

Evening primrose oil contains saturated fatty acid and unsaturated fatty acid, wherein high-carbon fatty acid such as oleic acid, linoleic acid, gamma-linolenic acid, etc. has high content, soft and elastic feeling to skin, good touch and ductility, and easy absorption by skin blood vessel, and can promote blood circulation and prevent epidermal cell keratinization. The evening primrose oil contains saturated fatty acid and unsaturated fatty acid, provides sufficient toughness for skin structure, and can restore normal barrier function of skin. The evening primrose oil is harmless and non-irritant to human bodies, and has a good auxiliary medical effect on burns and wounds.

The Pu' er tea fruit oil has high nutritive value, is rich in tea polyphenol, has strong antioxidant and anti-inflammatory effects, can improve the no-wash performance of a skin system, and simultaneously has good effect on improving the vitality of human bodies. The tea polyphenol has good reducibility, astringency and antibacterial property, and can be used for treating topical hemorrhage. In conclusion, the Pu' er tea fruit oil integrates the functions of antibiosis, anti-inflammation, antioxidation, hemostasis and skin protection.

The natural cellulose is polysaccharide which is most widely distributed and contained in nature, and has abundant sources. Current cellulose modification technologies focus primarily on both etherification and esterification. Carboxymethylation is one of the etherification techniques. Carboxymethyl cellulose (CMC) is obtained after carboxymethylation of cellulose, and the aqueous solution of the carboxymethyl cellulose has the functions of thickening, film forming, adhesion, water retention, colloid protection and the like. The high-end substitute product of carboxymethyl cellulose (CMC) is polyanionic cellulose (PAC) and anionic cellulose ether, has higher substitution degree and substitution uniformity, shorter molecular chain and more stable molecular structure, thereby having better comprehensive performance and enhanced solubility. The method is applied to all the industries where the carboxymethyl cellulose can be applied, and can provide better stability and meet higher process requirements. Poloxamers (poloxamers) are polyoxyethylene polyoxypropylene ether block copolymers available under the trade name Pluronic. The surfactant is a novel macromolecular nonionic surfactant. The combination of polyanionic cellulose (PAC) and poloxamer can effectively provide better stability for a reaction system, and simultaneously can enhance the adhesive property of medical glue and has the water retention property.

Due to the adoption of the technical scheme, the invention has the following beneficial effects:

1. wound healing is a biochemical and cytological process involving and interacting with inflammatory cells, epidermal cells, endothelial cells, fibroblasts and the like, and collagen synthesized and secreted by the fibroblasts is a main component of wound tissues. The medical adhesive with the functions of promoting healing and repairing contains important components capable of promoting the healing process in high efficiency and repairing components selected by repeated experiments, can effectively improve the wound healing rate, and has good effect on repairing scars. The jellyfish extract, the starfish extract, the gecko extract and the salamander extract which are selected as the special healing promoting and repairing components have strong function of regenerating amputated limbs, and the extracts also contain various components for promoting cell proliferation and important substances for promoting tissue repair.

Protein, amino acid, special polysaccharide and mineral substances in the natural components for promoting healing and repairing tricholoma matsutake extract are combined; proteins, amino acids, flavones, procyanidins and polysaccharides in the Lycium ruthenicum Murr extract; the cocoa seed extract is rich in cocoa molecular peptides, saccharides and polyphenols; the natural healing promoting and repairing component system can provide nutrition for the skin, promote healing, repair damaged cells and tissues in time and in high quality, and establish a protective layer for the skin.

Combining with active ingredients of cordycepin, protein, amino acid, sterol, etc. of Cordyceps extract in the Chinese medicinal composition for promoting healing and repairing; ganoderma lucidum polysaccharide, Ganoderma lucidum polypeptide, triterpene, alkaloid and various amino acids in Ganoderma sinensis extract, and Lumbricus extract, Lumbricus alkali, purine, choline, vitamins, amino acids, etc.; curcumin, turmerone, aromatic-turmerone, curcumene, aromatic-curcumene, sterols and the like in the turmeric extract form a strong restoration and repair capability system in a traditional Chinese medicine healing promotion and repair component system. The three major active ingredients supplement each other and cooperate with each other, thereby effectively achieving the purposes of efficiently promoting healing and repairing injured tissues.

2. The invention integrates animal protein, plant protein, protein in the compound fruit oil and the sterilization and anti-inflammatory effects of the animal protein and the plant protein, so that the sore wound on the skin can be quickly healed, the immunity of the skin is further improved, meanwhile, as fish skin collagen, wheat protein hydrolysate lycium ruthenicum and aloe also contain a large amount of protein and trace elements, the skin structure can be improved, the skin is compact, and a protective barrier is formed, and a plurality of proteins, amino acids and trace elements are organically combined together, so that the product has better effect.

3. The medical adhesive with the healing promoting and repairing functions selects sodium alginate, sodium hyaluronate, allantoin, hydrolyzed wheat protein, a stabilizer and other auxiliary components to comprehensively improve the long-acting and efficient healing promoting and repairing performance of the whole product, simultaneously improves the comprehensive mechanical property of the medical adhesive, and greatly reduces the risk of a user. The composite additive disclosed by the invention is combined with other components, so that the air permeability and the permeability of a final cosmetic product can be enhanced, and the repair effect of the final product is improved.

4. The composite fruit oil selected by the medical adhesive with the functions of promoting healing and repairing is prepared by the synergistic use of aloe oil, rose hip oil, evening primrose oil and Pu' er tea fruit oil, so that the whole fruit oil matrix participating in reaction can simultaneously and rapidly realize the repair of damaged cell tissues, promote cell regeneration and wound healing, promote blood circulation, nourish skin more deeply, improve and eliminate new and old scars after trauma, and simultaneously can establish the function of protecting barriers for new skin. The composite fruit oil composition provides the most stable foundation for promoting healing and repairing for the medical adhesive, can continuously and effectively enhance the skin resistance, effectively prevent oxidative damage, and simultaneously can repair the skin, improve scars and promote wound healing and recovery.

5. The medical glue with the functions of promoting healing and repairing is particularly added with allantoin, and has unique and important effects. Allantoin has the physiological functions of promoting cell growth, accelerating wound healing, softening keratin and the like, and is a good healing agent for skin wounds. In addition, allantoin can be used for alleviating and treating xerosis cutis and skin ulcer, and has good therapeutic effect on inflammation. The allantoin is an amphoteric compound, can be combined with various substances to form a double salt, has the effects of protecting from light, sterilizing, preventing corrosion, relieving pain and resisting oxidation, can keep the skin moisture, and is more moist and soft. The allantoin is used and simultaneously acts as a formaldehyde inhibitor in the system, and effectively acts as the formaldehyde inhibitor, so that the system does not need to additionally add a separate formaldehyde inhibitor and a preservative.

6. The curcumin contained in the curcuma extract in the medical gel with the functions of promoting healing and repairing can be combined with fish skin collagen, cordyceps sinensis extract, octopus polysaccharide, gecko extract and polysaccharide substances in starfish extract, and the curcumin is mutually cooperated to effectively realize the healing of wound surfaces.

7. Each additive component except the stabilizer selected by the medical adhesive with the healing promoting and repairing functions has efficient healing promoting and repairing functions, becomes an essential component of the whole healing promoting and repairing product, has natural effects of various healing promoting and repairing components, and is prepared into the medical adhesive with the healing promoting and repairing functions. Sodium alginate is a natural polysaccharide carbohydrate, and a medical material prepared by taking alginate as a raw material has good affinity with wounds, has the characteristic of rapid hemostasis, can be used for repairing tissues and organs of a human body, and has the performances of promoting healing and repairing. The main amino acids of wheat, gliadin and glutenin, are rich in cystine, which is a sulfur-containing amino acid. The hydrolyzed wheat protein can supplement protein nutrition, help skin repair and resist inflammation. The hyaluronic acid can prevent the generation of some enzymes in cells, reduce the formation of free radicals, play an important role in preventing the free radicals from damaging cell structures, generating lipid peroxidation, causing body aging and the like, provide a proper place for cell proliferation and differentiation, directly promote cell growth, differentiation, reconstruction, repair and the like, and have an excellent repair effect on damaged skin. Allantoin has the physiological functions of promoting cell growth, accelerating wound healing, softening keratin and the like, and is a good healing agent for skin wounds. In conclusion, all the selected added raw materials have the healing promoting and repairing performance, three components of specific healing promoting and repairing components, natural healing promoting and repairing components and traditional Chinese medicine healing promoting and repairing components are added on the basis, the healing promoting and repairing performance of the product is comprehensively improved by a repairing system, and the medical adhesive with the healing promoting and repairing functions has stronger and more efficient healing promoting and repairing performance and more complete comprehensive performance under the synergistic effect.

The medical adhesive with the functions of promoting healing and repairing has the bonding strength of more than 60N, the antibacterial rate to staphylococcus aureus of more than 97.7 percent, the overall healing efficiency of more than 98.5 percent, high biocompatibility, no stimulation to wound tissues, lowered sensitization rate, excellent comprehensive performance, accordance with the application requirement of clinical medical adhesive, and the mental and psychological pressure of patients can be reduced to the maximum extent by improving the healing promoting and repairing performances of the medical adhesive.

Detailed Description

The invention is described in detail below by means of specific examples:

example 1

A medical adhesive with healing promoting and repairing functions comprises the following components in parts by weight:

20 parts of n-butyl a-cyanoacrylate, 25 parts of isooctyl a-cyanoacrylate, 2 parts of styrene thermoplastic elastomer microspheres, 25 parts of efficient healing promoting and repairing components, 2 parts of sodium alginate, 3 parts of sodium hyaluronate, 8 parts of allantoin, 1 part of hydrolyzed wheat protein, 1 part of stabilizer, 150 parts of composite fruit oil component and 200 parts of sterile water; the high-efficiency healing promoting and repairing component comprises 6 parts of specific healing promoting and repairing component, 12 parts of natural healing promoting and repairing component and 6 parts of traditional Chinese medicine healing promoting and repairing component; the components for promoting healing and repairing specially comprise 2 parts of jellyfish extract, 2 parts of starfish extract, 1 part of gecko extract, 1 part of salamander, 4 parts of octopus polysaccharide and 4 parts of fish skin collagen in parts by weight; the natural healing promoting and repairing component comprises 2 parts of tricholoma matsutake extract, 2 parts of lycium ruthenicum extract, 3 parts of cocoa seed extract and 5 parts of kiwi fruit extract in parts by weight; the Chinese medicinal components for promoting healing and repairing comprise 0.5 part of Ganoderma sinensis extract, 2 parts of earthworm extract, 2 parts of Curcuma longa extract and 1.5 parts of Cordyceps sinensis extract in parts by weight; the composite fruit oil consists of aloe oil, rose hip oil, evening primrose oil and Pu' er tea fruit oil according to the weight percentage of 5:3:2: 1.

The stabilizer is polyanionic cellulose and poloxamer according to the weight part of 5: 1, combining the components; starfishes in the starfish extract consist of asterias rollestoni, asterias amurensis and starfish in a weight ratio of 1:1: 1; the particle size range of the styrene thermoplastic elastomer microspheres is 50nm-3 mu m, and the particle size distribution is less than 0.5.

The medical adhesive prepared by the embodiment is used for smearing wounds, and relevant performance tests are carried out, wherein the test results are as follows: the medical adhesive with the functions of promoting healing and repairing has the bonding strength of 61N, the antibacterial rate to staphylococcus aureus reaches more than 97.7%, and the overall healing rate reaches 98.5%.

Example 2

A medical adhesive with healing promoting and repairing functions comprises the following components in parts by weight:

22 parts of a-n-butyl cyanoacrylate, 28 parts of a-isooctyl cyanoacrylate, 4 parts of styrene thermoplastic elastomer microspheres, 30 parts of efficient healing promotion and repair components, 3 parts of sodium alginate, 4 parts of sodium hyaluronate, 9 parts of allantoin, 1 part of hydrolyzed wheat protein, 1 part of stabilizer, 180 parts of composite fruit oil component and 200 parts of sterile water; the high-efficiency healing promoting and repairing component comprises 6 parts of specific healing promoting and repairing component, 17 parts of natural healing promoting and repairing component and 9 parts of traditional Chinese medicine healing promoting and repairing component; the components for promoting healing and repairing specially comprise 2 parts of jellyfish extract, 2 parts of starfish extract, 1 part of gecko extract, 1 part of salamander, 4 parts of octopus polysaccharide and 4 parts of fish skin collagen in parts by weight; the natural healing promoting and repairing component comprises 2 parts of tricholoma matsutake extract, 2 parts of lycium ruthenicum extract, 3 parts of cocoa seed extract and 5 parts of kiwi fruit extract in parts by weight; the Chinese medicinal components for promoting healing and repairing comprise 0.5 part of Ganoderma sinensis extract, 2 parts of earthworm extract, 2 parts of Curcuma longa extract and 1.5 parts of Cordyceps sinensis extract in parts by weight; the composite fruit oil consists of aloe oil, rose hip oil, evening primrose oil and Pu' er tea fruit oil according to the weight percentage of 5:3:2: 1.

The stabilizer is polyanionic cellulose and poloxamer according to the weight part of 5: 1, combining the components; starfishes in the starfish extract consist of asterias rollestoni, asterias amurensis and starfish in a weight ratio of 1:1: 1; the particle size range of the styrene thermoplastic elastomer microspheres is 50nm-3 mu m, and the particle size distribution is less than 0.5.

The medical adhesive prepared by the embodiment is used for smearing wounds, and relevant performance tests are carried out, wherein the test results are as follows: the medical adhesive with the functions of promoting healing and repairing has the bonding strength of 62N, the antibacterial rate to staphylococcus aureus of more than 98 percent and the overall healing rate of 99 percent.

Example 3

A medical adhesive with healing promoting and repairing functions comprises the following components in parts by weight:

24 parts of n-butyl a-cyanoacrylate, 33 parts of isooctyl a-cyanoacrylate, 5 parts of styrene thermoplastic elastomer microspheres, 35 parts of efficient healing promoting and repairing components, 4 parts of sodium alginate, 5 parts of sodium hyaluronate, 10 parts of allantoin, 1 part of hydrolyzed wheat protein, 1 part of stabilizer, 210 parts of composite fruit oil component and 200 parts of sterile water; the high-efficiency healing promoting and repairing component comprises 6 parts of specific healing promoting and repairing component, 22 parts of natural healing promoting and repairing component and 10 parts of traditional Chinese medicine healing promoting and repairing component; the components for promoting healing and repairing specially comprise 2 parts of jellyfish extract, 2 parts of starfish extract, 1 part of gecko extract, 1 part of salamander, 4 parts of octopus polysaccharide and 4 parts of fish skin collagen in parts by weight; the natural healing promoting and repairing component comprises 2 parts of tricholoma matsutake extract, 2 parts of lycium ruthenicum extract, 3 parts of cocoa seed extract and 5 parts of kiwi fruit extract in parts by weight; the Chinese medicinal components for promoting healing and repairing comprise 0.5 part of Ganoderma sinensis extract, 2 parts of earthworm extract, 2 parts of Curcuma longa extract and 1.5 parts of Cordyceps sinensis extract in parts by weight; the composite fruit oil consists of aloe oil, rose hip oil, evening primrose oil and Pu' er tea fruit oil according to the weight percentage of 5:3:2: 1.

The stabilizer is polyanionic cellulose and poloxamer according to the weight part of 5: 1, combining the components; starfishes in the starfish extract consist of asterias rollestoni, asterias amurensis and starfish in a weight ratio of 1:1: 1; the particle size range of the styrene thermoplastic elastomer microspheres is 50nm-3 mu m, and the particle size distribution is less than 0.5.

The medical adhesive prepared by the embodiment is used for smearing wounds, and relevant performance tests are carried out, wherein the test results are as follows: the medical adhesive with the functions of promoting healing and repairing has the bonding strength of 61.5N, the antibacterial rate to staphylococcus aureus of more than 98.2 percent and the overall healing rate of 99 percent.

Example 4

A medical adhesive with healing promoting and repairing functions comprises the following components in parts by weight:

25 parts of n-butyl a-cyanoacrylate, 35 parts of isooctyl a-cyanoacrylate, 6 parts of styrene thermoplastic elastomer microspheres, 37 parts of efficient healing promoting and repairing components, 4 parts of sodium alginate, 6 parts of sodium hyaluronate, 10 parts of allantoin, 1 part of hydrolyzed wheat protein, 1 part of stabilizer, 220 parts of composite fruit oil component and 200 parts of sterile water; the high-efficiency healing promoting and repairing component comprises 6 parts of specific healing promoting and repairing component, 24 parts of natural healing promoting and repairing component and 12 parts of traditional Chinese medicine healing promoting and repairing component; the components for promoting healing and repairing specially comprise 2 parts of jellyfish extract, 2 parts of starfish extract, 1 part of gecko extract, 1 part of salamander, 4 parts of octopus polysaccharide and 4 parts of fish skin collagen in parts by weight; the natural healing promoting and repairing component comprises 2 parts of tricholoma matsutake extract, 2 parts of lycium ruthenicum extract, 3 parts of cocoa seed extract and 5 parts of kiwi fruit extract in parts by weight; the Chinese medicinal components for promoting healing and repairing comprise 0.5 part of Ganoderma sinensis extract, 2 parts of earthworm extract, 2 parts of Curcuma longa extract and 1.5 parts of Cordyceps sinensis extract in parts by weight; the composite fruit oil consists of aloe oil, rose hip oil, evening primrose oil and Pu' er tea fruit oil according to the weight percentage of 5:3:2: 1.

The stabilizer is polyanionic cellulose and poloxamer according to the weight part of 5: 1, combining the components; starfishes in the starfish extract consist of asterias rollestoni, asterias amurensis and starfish in a weight ratio of 1:1: 1; the particle size range of the styrene thermoplastic elastomer microspheres is 50nm-3 mu m, and the particle size distribution is less than 0.5.

The medical adhesive prepared by the embodiment is used for smearing wounds, and relevant performance tests are carried out, wherein the test results are as follows: the medical adhesive with the functions of promoting healing and repairing has the bonding strength of 62N, the antibacterial rate to staphylococcus aureus of more than 98 percent and the overall healing rate of 99 percent.

In the above embodiments

The preparation method of jellyfish extract and starfish extract comprises the following steps:

respectively taking freshly collected jellyfish and starfish as raw materials, cleaning the raw materials, shearing the raw materials, adding 95% ethanol solution which is 6 times of the weight of the raw materials according to the weight-volume ratio, and performing reflux extraction for 3 times, 6 hours each time; mixing extractive solutions, and recovering solvent to obtain jellyfish extract and starfish extract.

The cocoa seed extract is prepared by the following steps:

drying and pulverizing cacao seeds, sieving with 100 mesh sieve to obtain powder of cacao seeds, adding 95% ethanol solution, and ultrasonic extracting at 45 deg.C and 200W for 35min to obtain extractive solution; filtering the extractive solution, and concentrating under reduced pressure to obtain slurry; the slurry was mixed at a ratio of 1: dissolving the extract in deionized water at a volume ratio of 15, stirring, shaking, and sterilizing with microporous membrane to obtain cacao seed extract.

The preparation method of the kiwi fruit extract comprises the following steps:

the kiwi fruit extract is an ethanol extract of kiwi fruit, and the specific extraction steps are as follows: pulverizing dried fructus Actinidiae chinensis, sieving with 60 mesh sieve, adding 17 times of 90% ethanol solution, soaking for 50 hr, filtering, and centrifuging at 3000 rpm/min for 10 min; collecting supernatant, concentrating by rotary evaporation to concentration of 1.5g/ml, and sterilizing the concentrated solution with microporous membrane device to obtain fructus Actinidiae chinensis extract.

The preparation method of Tricholoma matsutake extract comprises:

slicing wild matsutake, drying to obtain matsutake air-dried slices, pulverizing matsutake air-dried slices, adding into 90% ethanol solution at a mass ratio of 1:6, soaking for 40 hr, extracting under reflux for 8 hr, refining the obtained heat reflux extract, recovering ethanol, concentrating under reduced pressure, filtering, concentrating the obtained filtrate under reduced pressure, refining to obtain solution B, dissolving the filtered insoluble substance in butanediol aqueous solution, refining to obtain solution A, mixing solution A and solution B, and storing at 1 deg.C for 50 hr to obtain matsutake extract.

The preparation method of the lycium ruthenicum extract comprises the following steps:

washing away silt on the surface of the lycium ruthenicum by using running water, introducing an ozone generator into sterile water to wash the lycium ruthenicum again, crushing pulp into pulp, adding deionized water according to the volume of 30% of the pulp, adding 2% of pectinase at the same time, uniformly stirring, and standing for 50min at 33 ℃; adding the supernatant into 95% ethanol solution, ultrasonic extracting at 40 deg.C and 200W ultrasonic power for 20min, and centrifuging at 4000rpm/min for 10 min; after concentrating the supernatant from the centrifugation to a semi-fluid slurry, the slurry was mixed at a ratio of 1: dissolving the extract in deionized water at a volume ratio of 20, stirring, shaking, and sterilizing with microporous membrane to obtain Lycium ruthenicum Murr extract.

The method comprises the following specific extraction steps of:

respectively pulverizing dried Ganoderma, Lumbricus, Curcuma rhizome and Cordyceps, sieving with 200 mesh sieve, adding 90% ethanol solution 20 times the weight of the powder, soaking for 60 hr, filtering, and centrifuging at 3000 rpm/min for 10 min; collecting supernatant, concentrating by rotary evaporation to concentration of 1g/ml, and sterilizing the concentrated solution with microporous membrane filter to obtain Ganoderma extract, Lumbricus extract, Curcuma rhizome extract and Cordyceps extract.

The preparation method of the medical glue with the functions of promoting healing and repairing comprises the following steps:

step one, adding sodium alginate, sodium hyaluronate, allantoin, hydrolyzed wheat protein and a stabilizer into sterile water according to a part ratio, heating to 75 ℃, stirring for dissolving for 150min, and cooling to room temperature to obtain an aqueous solution A;

step two, putting the a-n-butyl cyanoacrylate, the a-isooctyl cyanoacrylate and the styrene thermoplastic elastomer microspheres into the composite fruit oil according to the parts ratio, heating to 70 ℃, stirring for 80min, and cooling to room temperature to obtain a composite fruit oil solution B;

step three, uniformly mixing the aqueous solution A and the composite fruit oil solution B, adding efficient healing promoting and repairing components according to the parts ratio, and stirring for 3.5 hours;

and step four, filtering and sterilizing the product obtained in the step three, subpackaging, sealing and packaging to obtain the medical adhesive with the healing promoting and repairing functions.

Performance testing

The medical adhesive prepared by the embodiment is used for smearing the wound, the bonding strength of the medical adhesive with the functions of promoting healing and repairing is greater than 60N, the antibacterial rate of staphylococcus aureus is up to more than 97.7%, the overall healing efficiency is up to more than 98.5%, the biocompatibility is high, the medical adhesive does not irritate wound tissues, the sensitization rate is reduced, the comprehensive performance is excellent, the medical adhesive meets the clinical application requirements of the medical adhesive, and the mental and psychological pressure of a patient is reduced to the maximum extent by improving the healing promoting and repairing performances of the medical adhesive.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims (1)

1. A preparation method of medical glue with healing promotion and repair functions is characterized in that:

the medical adhesive comprises the following components in parts by weight: 20-25 parts of a-n-butyl cyanoacrylate, 25-35 parts of a-isooctyl cyanoacrylate, 2-6 parts of styrene thermoplastic elastomer microspheres, 25-37 parts of efficient healing promoting and repairing components, 2-4 parts of sodium alginate, 3-6 parts of sodium hyaluronate, 8-10 parts of allantoin, 1 part of hydrolyzed wheat protein, 1 part of stabilizer, 220 parts of compound fruit oil component 150 and 200 parts of sterile water;

the high-efficiency healing promoting and repairing component comprises 6 parts of specific healing promoting and repairing component, 12-24 parts of natural healing promoting and repairing component and 6-12 parts of traditional Chinese medicine healing promoting and repairing component;

the components for promoting healing and repairing specially comprise 2 parts of jellyfish extract, 2 parts of starfish extract, 1 part of gecko extract, 1 part of salamander essence, 4 parts of octopus polysaccharide and 4 parts of fish skin collagen in parts by weight;

the natural healing promoting and repairing component comprises 2 parts of tricholoma matsutake extract, 2 parts of lycium ruthenicum extract, 3 parts of cocoa seed extract and 5 parts of kiwi fruit extract in parts by weight;

the traditional Chinese medicine components for promoting healing and repairing comprise, by weight, 0.5 part of ganoderma sinensis extract, 2 parts of earthworm extract, 2 parts of turmeric extract and 1.5 parts of cordyceps sinensis extract;

the composite fruit oil component consists of aloe oil, rose hip oil, evening primrose oil and Pu' er tea fruit oil according to the weight fraction of 5:3:2: 1;

the stabilizer is polyanionic cellulose and poloxamer according to the weight part ratio of 5: 1, combining the components;

the starfishes in the starfish extract consist of asterias rollestoni, asterias amurensis and the starfish in a weight ratio of 1:1: 1; the particle size range of the styrene thermoplastic elastomer microspheres is 50nm-3 mu m, and the particle size distribution is less than 0.5;

the preparation method comprises the following steps:

step one, adding sodium alginate, sodium hyaluronate, allantoin, hydrolyzed wheat protein and a stabilizer into sterile water according to a part ratio, heating to 75 ℃, stirring for dissolving for 150min, and cooling to room temperature to obtain an aqueous solution A;

step two, putting the a-n-butyl cyanoacrylate, the a-isooctyl cyanoacrylate and the styrene thermoplastic elastomer microspheres into the composite fruit oil according to the parts ratio, heating to 85 ℃, stirring for 70min, and cooling to room temperature to obtain a composite fruit oil solution B;

step three, uniformly mixing the aqueous solution A and the composite fruit oil solution B, adding efficient healing promoting and repairing components according to the parts ratio, and stirring for 3.5 hours;

and step four, filtering and sterilizing the product obtained in the step three, subpackaging, sealing and packaging to obtain the medical adhesive with the healing promoting and repairing functions.