CN108603185B - 酶变体以及编码它们的多核苷酸 - Google Patents

酶变体以及编码它们的多核苷酸 Download PDF

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Publication number
CN108603185B
CN108603185B CN201680076825.9A CN201680076825A CN108603185B CN 108603185 B CN108603185 B CN 108603185B CN 201680076825 A CN201680076825 A CN 201680076825A CN 108603185 B CN108603185 B CN 108603185B
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CN108603185A (zh
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T.B.雷盖拉
B.普莱斯纳
T.H.布利克
A.D.霍格
S.阿内赫德
L.L.H.克里斯滕森
C.安德森
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Novozymes AS
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Novozymes AS
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Priority to CN202411263543.2A priority patent/CN119120430A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
    • C11D2111/10—Objects to be cleaned
    • C11D2111/12—Soft surfaces, e.g. textile
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
    • C11D2111/10—Objects to be cleaned
    • C11D2111/14—Hard surfaces
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
CN201680076825.9A 2015-12-30 2016-12-29 酶变体以及编码它们的多核苷酸 Active CN108603185B (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202210558733.1A CN114921442B (zh) 2015-12-30 2016-12-29 酶变体以及编码它们的多核苷酸
CN202411263543.2A CN119120430A (zh) 2015-12-30 2016-12-29 酶变体以及编码它们的多核苷酸

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP15203051 2015-12-30
EP15203051.6 2015-12-30
EP16168114 2016-05-03
EP16168114.3 2016-05-03
PCT/EP2016/082828 WO2017114891A1 (en) 2015-12-30 2016-12-29 Enzyme variants and polynucleotides encoding the same

Related Child Applications (2)

Application Number Title Priority Date Filing Date
CN202411263543.2A Division CN119120430A (zh) 2015-12-30 2016-12-29 酶变体以及编码它们的多核苷酸
CN202210558733.1A Division CN114921442B (zh) 2015-12-30 2016-12-29 酶变体以及编码它们的多核苷酸

Publications (2)

Publication Number Publication Date
CN108603185A CN108603185A (zh) 2018-09-28
CN108603185B true CN108603185B (zh) 2022-05-17

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Family Applications (3)

Application Number Title Priority Date Filing Date
CN202411263543.2A Pending CN119120430A (zh) 2015-12-30 2016-12-29 酶变体以及编码它们的多核苷酸
CN201680076825.9A Active CN108603185B (zh) 2015-12-30 2016-12-29 酶变体以及编码它们的多核苷酸
CN202210558733.1A Active CN114921442B (zh) 2015-12-30 2016-12-29 酶变体以及编码它们的多核苷酸

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CN202411263543.2A Pending CN119120430A (zh) 2015-12-30 2016-12-29 酶变体以及编码它们的多核苷酸

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Country Status (8)

Country Link
US (3) US11407986B2 (enExample)
EP (2) EP3397761B1 (enExample)
JP (2) JP7626576B2 (enExample)
CN (3) CN119120430A (enExample)
CA (1) CA3006607A1 (enExample)
MX (2) MX2018007485A (enExample)
WO (1) WO2017114891A1 (enExample)
ZA (1) ZA201803706B (enExample)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7626576B2 (ja) 2015-12-30 2025-02-07 ノボザイムス アクティーゼルスカブ 酵素変異体およびこれをコードするポリヌクレオチド
EP3241890B1 (en) * 2016-05-03 2019-06-26 The Procter and Gamble Company Automatic dishwashing detergent composition
WO2017191160A1 (en) * 2016-05-03 2017-11-09 Novozymes A/S Alpha-amylase variants and polynucleotides encoding the same
CN111212906B (zh) * 2017-08-18 2024-02-02 丹尼斯科美国公司 α-淀粉酶变体
KR101991684B1 (ko) * 2017-09-29 2019-09-30 오스템임플란트 주식회사 상아세관 침투력 및 코팅 유지력이 향상된 치아 도포제 및 이의 제조방법
CA3108284A1 (en) * 2018-07-31 2020-02-06 Danisco Us Inc Variant alpha-amylases having amino acid substitutions that lower the pka of the general acid
MX2021011287A (es) * 2019-03-21 2021-10-13 Novozymes As Variantes de alfa-amilasa y polinucleotidos que codifican las mismas.
CN114787329A (zh) * 2019-08-27 2022-07-22 诺维信公司 洗涤剂组合物
US12410385B2 (en) * 2019-10-24 2025-09-09 The Procter & Gamble Company Automatic dishwashing detergent composition comprising an amylase
WO2021123184A2 (en) * 2019-12-19 2021-06-24 Novozymes A/S Alpha-amylase variants
WO2021148364A1 (en) * 2020-01-23 2021-07-29 Novozymes A/S Enzyme compositions and uses thereof
WO2022175435A1 (en) * 2021-02-22 2022-08-25 Basf Se Amylase variants
EP4047088A1 (en) * 2021-02-22 2022-08-24 Basf Se Amylase variants
CN113151329B (zh) * 2021-03-30 2023-09-08 云南师范大学 中性蛋白酶突变体及其构造方法和应用
CN114381448B (zh) * 2022-01-10 2024-02-20 鑫缘茧丝绸集团股份有限公司 一种葡聚糖酶突变体及其应用
PE20251535A1 (es) 2022-05-14 2025-06-05 Novozymes As Composiciones y metodos para prevenir, tratar, suprimir y/o eliminar infecciones e infestaciones fitopatogenas
CN119677846A (zh) * 2022-08-11 2025-03-21 巴斯夫欧洲公司 淀粉酶变体

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