CN108567093A - 一种多穗柯提取物泡腾片及其制备方法 - Google Patents
一种多穗柯提取物泡腾片及其制备方法 Download PDFInfo
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- CN108567093A CN108567093A CN201810358613.0A CN201810358613A CN108567093A CN 108567093 A CN108567093 A CN 108567093A CN 201810358613 A CN201810358613 A CN 201810358613A CN 108567093 A CN108567093 A CN 108567093A
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- pasania cuspidata
- grain
- effervescent tablet
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
- A23L2/395—Dry compositions in a particular shape or form
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/30—Further treatment of dried tea extract; Preparations produced thereby, e.g. instant tea
- A23F3/32—Agglomerating, flaking or tabletting or granulating
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/40—Effervescence-generating compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/20—Agglomerating; Granulating; Tabletting
- A23P10/28—Tabletting; Making food bars by compression of a dry powdered mixture
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Botany (AREA)
- Mycology (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
本发明提供一种多穗柯提取物泡腾片及其制备方法,采用多穗柯毛绿茶为原料,用热水浸提其活性成分,添加辅料制成酸粒、碱粒,并对酸粒、碱粒的配方进行优化,得到既保留多穗柯中活性成分,又能快速溶解的新型固体饮料;将茶饮料与泡腾产品结合,既可以保留茶的清香,充分利用茶有效成分清热解毒、明目提神等,又可以充分利用湖南雪峰山地区丰富的多穗柯资源,增加原料的附加值,提升茶叶的利用价值。
Description
技术领域
本发明属于食品领域,涉及一种多穗柯提取物泡腾片及其制备方法。
背景技术
多穗柯俗称甜茶,壳斗科石柯属植物,主要以野生形式零星分布于我国长江以南各省区的低山密林中[1],湖南省怀化市是多穗柯的主要生产地之一。多穗柯产地的群众千百年来都采其嫩叶作甜茶,茶香浓郁,色泽鲜艳,回味甘甜持久,风味独特。多穗柯的化学成分主要为黄酮类和三萜类化合物,目前已从多穗柯叶部位地上部分分离鉴定出至少22种黄酮类成分,该类物质主要分布于叶部位,是多穗柯主要甜味成份[2-4]。多穗柯在我国南方具有悠久的应用历史,随着对其研究和认识的加深。多穗柯较强的降血脂、减肥、降低胆固醇、消食去腻、降血糖、抗过敏、抑菌、抗氧化、改善记忆能力等功效已逐步被人民认识和认可[5]。
泡腾片是指含有碳酸氢钠和有机酸,遇水会产生大量二氧化碳而迅速溶解呈泡腾状的片剂[6],具有携带和使用都方便的特点,在水中迅速溶解,有效成分便于吸收,并且生物利用度高,兼具固体制剂和液体制剂的特点,在药品、食品和农药行业具有很广阔的市场前景[7]。
近年来,多穗柯已走出民间,形成了商品化,各种散装茶和袋泡茶已在市场上流通,但对多穗柯的研究依然还不够深,这也限制了其应用和推广,需要从多个角度进行综合和可持续发展研究,从而使其得到更好的开发和利用[8-9]。
参考文献
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[9]宿迷菊,毛志方,施海根,等.食用泡腾片的制备及研究概况[J].中国茶叶加工,2008,5(2):20-23.[10]罗延红,廉秀娟,李引乾,等.泡腾片研究进展[J].西北药学杂志,2001,16(1):39-40.
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[12]王宁,陈学峰,王锐平.茶饮料泡腾片的加工工艺[J].食品与发酵工业,2007,23(3):151-153.
[13]王宁,陈学峰,王锐平.山楂泡腾片生产工艺研究[J].食品与发酵工业,2006,6(8):128-130.
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[15]周斌星,王燕,周增志,等.普洱茶(熟茶)提取物泡腾片主体配方的研究[J].安徽农业
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发明内容
本发明的目的在于提供一种多穗柯提取物泡腾片的制备方法,既保留多穗柯中活性成分,又能快速溶解的新型固体饮料,提升多穗柯的附加值。
多穗柯提取物泡腾片的制备方法,包括以下步骤:
(1)多穗柯提取物干粉制备:称取30g多穗柯毛绿茶,粉碎,过20~40目筛,加入质量分数分别为0.5%、1%的抗坏血酸和β-CD,加入300mL80℃温水浸提15min,趁热抽滤,滤渣用沸水洗涤,合并滤液,浓缩至浸提液黏稠得黏液,往其中加入黏液质量40%的麦芽糊精作填充剂,干燥10h,得多穗柯提取物干粉;
(2)酸粒制备:将多穗柯提取物干粉、柠檬酸、酒石酸、木糖醇和阿斯巴甜混合,粉碎,用质量浓度1%聚乙烯吡咯烷酮的无水乙醇润湿,50℃烘干,制粒机制粒,得酸粒;
(3)碱粒制备:将多穗柯提取物干粉、碳酸钠和碳酸氢钠混合,粉碎,50℃烘干,制粒机制粒,得碱粒;
(4)将酸粒、碱粒混合,添加酸粒和碱粒总质量1%的L-HPC,压片机压片,即得成品。
进一步,酸粒中有机酸柠檬酸和酒石酸质量分数为1:1。
进一步,酸粒添加量为成品质量的8%、9%、10%、11%或12%。
进一步,碱粒中碱性物质碳酸钠、碳酸氢钠质量分数1:1。
进一步,碱粒添加量为为成品质量的5%、5.5%、6%、6.5%或7%。
进一步,酒石酸、柠檬酸、碳酸钠、木糖醇、碳酸氢钠、阿斯巴甜、麦芽糊精、抗坏血酸、β-CD、聚乙烯吡咯烷酮、低取代羟丙基纤维素、无水乙醇为食品级。
进一步,步骤(1)中采用旋转蒸发仪将合并滤液浓缩至浸提液黏稠。
本发明采用多穗柯毛绿茶为原料,用热水浸提其活性成分,添加辅料制成酸粒、碱粒,并对酸粒、碱粒的配方进行优化,得到既保留多穗柯中活性成分,又能快速溶解的新型固体饮料,提升多穗柯的附加值,丰富其产品形式,为农民创收。将茶饮料与泡腾产品结合,既可以保留茶的清香,充分利用茶有效成分清热解毒、明目提神等,又可以充分利用湖南雪峰山地区丰富的多穗柯资源,增加原料的附加值,提升茶叶的利用价值;结合泡腾片本身的诸多优点,适应市场需求,满足消费者,尤其是新一代青年消费群体的求新、求异的需求,市场前景广阔。
附图说明
图1为本发明的工艺流程图
图2.1为多穗柯提取物泡腾片成品图
图2.2为芦丁标准曲线图
图2.3为多穗柯提取物泡腾片中总黄酮含量
图2.4为多穗柯提取物泡腾片中茶多酚含量测定
具体实施方式
为使本发明的目的、技术方案和优点更加清楚,下面本发明中的技术方案进行清楚、完整地描述,显然,所描述的实施例是本发明一部分实施例,而不是全部的实施例。基于本发明中的实施例,本领域普通技术人员在没有作出创造性劳动前提下所获得的所有其他实施例,都属于本发明保护的范围。
如图1所示,本发明的多穗柯提取物泡腾片的制备方法具体步骤如下:
(1)多穗柯提取物干粉制备:称取30.00g多穗柯毛绿茶,粉碎,过20~40目筛,加入质量分数分别为0.5%、1%的抗坏血酸和β-CD[12],加入300mL80℃温水浸提15min,趁热抽滤,滤渣用沸水洗涤,合并滤液,旋转蒸发仪浓缩至浸提液黏稠,往其中加入40%麦芽糊精作填充剂,干燥10h,得出多穗柯提取物干粉。
(2)酸粒制备:将多穗柯提取物干粉、柠檬酸、酒石酸、木糖醇、阿斯巴甜等混合,粉碎,用少量1%PVP的无水乙醇润湿[13],50℃烘干,制粒机制粒,得酸粒。
(3)碱粒制备:将多穗柯提取物干粉、碳酸钠、碳酸氢钠等混合,粉碎,50℃烘干,制粒机制粒,得碱粒。
(4)将酸粒、碱粒混合,添加1%的L-HPC,压片机压片,即得成品[14-15]。
1实验材料
多穗柯毛绿茶,购于怀化市溆浦县君健中药材专业合作社。
酒石酸、柠檬酸、碳酸钠、木糖醇、碳酸氢钠、阿斯巴甜、麦芽糊精、抗坏血酸、β-环状糊精、聚乙烯吡咯烷酮、低取代羟丙基纤维素、无水乙醇,食品级,购于杭州临安金龙化工有限公司。
磷酸二氢钾、酒石酸亚铁、磷酸氢二钠、酒石酸钾钠、芦丁、硝酸钠,分析纯,购于贵州迪大科技有限公司。
2.1有机酸添加量优化
酸粒中有机酸由柠檬酸和酒石酸按1:1组成,改变其添加量为8%、9%、10%、11%、12%,其它工艺同多穗柯提取物泡腾片制备工艺流程,根据评分结果,选择适宜的有机酸添加量。
2.2碱性物质添加量优化
碱粒中碱性物质由碳酸钠、碳酸氢钠按1:1组成,改变其添加量为5%、5.5%、6%、6.5%、7%,其它工艺同多穗柯提取物泡腾片制备工艺流程,根据评分结果,选择适宜碱性物质添加量。
3多穗柯提取物泡腾片理化指标检测
3.1总黄酮含量测定
(1)芦丁标准曲线的制备
精密称取减压干燥至恒重的芦丁10mg,置50mL容量瓶中,加入30mL 85%乙醇,水浴使其溶解并放置冷却至室温,加乙醇稀释至刻度,然后摇匀。使之成为浓度为0.2mg/mL的芦丁标准品溶液,作为贮备液备用。精密吸取此对照液0、0.5、1.0、2.0、3.0、4.0、5.0mL分别置于25mL量瓶中,加5%亚硝酸钠溶液1.0mL,摇匀放置6min后,加10%氯化铝溶液1.0mL,摇匀。放置6min后,加入1.0moL/LNaOH 10mL,最后各用85%乙醇稀释至刻度并摇匀,放置15min。第一瓶作空白,在510nm处测吸光度,以芦丁质量(mg)作横坐标,吸光度(OD510nm)作纵坐标,绘制标准曲线,计算回归方程。
(2)多穗柯提取物泡腾片中总黄酮含量测定
称取1g泡腾片成品,加入乙醇溶液浸泡24h后,进行超声波提取,过滤,蒸发回收乙醇,加水定容至25mL,得样品溶液,备用。精密吸取1mL样品溶液至25mL的定量管,后续同标准曲线制备,测定OD510nm,计算样品中总黄酮含量[16]。
3.2茶多酚含量测定
称取1g泡腾片成品入250mL烧杯中,加入100mL水,溶解,得样品匀液,称取其总质量,然后置于电炉上加热至沸腾10min,将二氧化碳气排除,冷却后,用水补足其原来的质量,摇匀,备用。
精确称取上述制备的试液5g于25mL容量瓶,加水4mL酒石酸亚铁溶液5mL,充分摇匀,用pH7.5磷酸缓冲溶定容至刻度液。用比色皿测定样品的OD540nm,以试剂空白做参比,测定其吸光度(A1),同时称取等量的试液于25mL容量瓶中,加水4mL,用pH7.5磷酸缓冲溶液定容至刻度测定其吸光度(A2),以试剂空白做参比。
X—样品中茶多酚的含量,mg/kg;
k:稀释倍数;
M:样品质量,g。
4结果与分析
4.1多穗柯提取物干燥工艺优化
不同干燥方法对多穗柯干粉的感官评价结果如表2.1所示。
表2.1不同干燥方法对多穗柯干粉的感官评价结果
由表2.1可知,鼓风干燥制的的干粉难以压片,压片成品相互粘结在压片机上,导致成品压片效果差,并且成品溶解速度差,沉淀较多;真空干燥较鼓风干燥容易压制成片,但产品溶解速度较慢并且带有不溶物较多的现象。这可能是因为鼓风干燥和真空干燥温度太高,使其中的糖类物质溶化,从而导致制备的干粉粘度太大,使压片无法正常进行。冷冻干燥得到的干粉疏松多孔,易于在水中迅速溶解,压制干粉效果最好,故选择冷冻干燥对多穗柯提取物进行干制。
4.2多穗柯提取物泡腾片制备工艺优化
4.2.1有机酸添加量优化
有机酸添加量对多穗柯提取物泡腾片感官评价影响结果如表2.2所示。
表2.2有机酸添加量对产品的影响
由表2.2可知:当有机酸添加量在8%~10%时,所制成的产品甜度减小,酸味增强,当添加量为10%时,所制成的产品口味酸甜适中,溶解性好,崩解速度快。但有机酸添加量超过10%时,所制成的产品酸味过重,并且产品中产生较多的不溶物,这可能是因为有机酸添加量过低会导致产气过少,影响产品崩解速度。有机酸添加量过高则会影响产品口感,并且会导致产品酸性物质过量而产生沉淀。故选择最优有机酸添加量为10%。
4.2.2碱性物质添加量优化
碱性物质添加量对多穗柯提取物泡腾片的影响结果如表2.3所示。
表2.3碱性物质添加量对产品的影响
由表2.3可知,碱性物质添加量为5%~6.5%,酸味逐渐减弱,崩解速度逐渐变快,产品的口感体验慢慢上升,溶解性也升高;当添加量达到6.5%时,所制成产品溶解效果好,崩解速度快,口味好;但碱性物质添加量超过6.5%时,崩解速度不再增快,且产品口感会出现涩味。碱性物质添加量过低与过低均会会影响产品口感,其原因可能是由于:酸过量易出现沉淀,碱性物质添加量过低则导致产品酸碱反应不充分,影响产品的崩解速度,碱性物质添加过量时,碱味会对产品产生较为强烈的覆盖。故选择最优碱性物质添加量为6.5%。
4.3多穗柯提取物泡腾片感官评价
按优化后的工艺制备多穗柯提取物泡腾片成品如图2.1所示,由图2.1所知,成品呈圆形,黄色,表面光滑,硬度适中。将其分别溶解于水中,崩解时间如表2.4所示。
表2.4成品质量与崩解时间
由表2.4可以看出,采用优化工艺所制成的穗柯提取物泡腾片,崩解速度快且质量比较均一。
4.4多穗柯提取物泡腾片理化指标检测
4.4.1总黄酮含量测定
4.4.1.1芦丁标准曲线制备
芦丁标准曲线如图2.2所示,在浓度0.6~0.8mg/kg范围之内,OD510nm与芦丁浓度线性关系良好,回归方程为y=0.3516x-0.0068,R2=0.9976。
4.4.1.2多穗柯提取物泡腾片中总黄酮含量测定
多穗柯提取物泡腾片中总黄酮含量测定结果如图2.3所示,多穗柯提取物泡腾片中总黄酮含量在410mg/kg左右。
4.4.2茶多酚含量测定
多穗柯提取物泡腾片中茶多酚含量测定结果如图2.4所示,多穗柯提取物泡腾片中茶多酚含量平均420mg/kg,GB/T31766-2008中要求茶汤中最低限量300mg/kg,本产品符合国标要求。
5结论
对多穗柯提取物泡腾片的主体配方进行优化,得出有机酸添加量10%、碱性物质添加量6.5%为最优结果,按该配方生产的多穗柯提取物泡腾片溶解速度快,沉淀较少、酸甜适中,茶多酚含量为420mg/kg,总黄酮含量为410mg/kg。
最后应说明的是:以上实施例仅用以说明本发明的技术方案,而非对其限制;尽管参照前述实施例对本发明进行了详细的说明,本领域的普通技术人员应当理解:其依然可以对前述各实施例所记载的技术方案进行修改,或者对其中部分技术特征进行等同替换;而这些修改或者替换,并不使相应技术方案的本质脱离本发明各实施例技术方案的精神和范围。
Claims (8)
1.多穗柯提取物泡腾片的制备方法,其特征在于,包括以下步骤:
(1)多穗柯提取物干粉制备:称取30g多穗柯毛绿茶,粉碎,过20~40目筛,加入质量分数分别为0.5%、1%的抗坏血酸和β-CD,加入300mL80℃温水浸提15min,趁热抽滤,滤渣用沸水洗涤,合并滤液,浓缩至浸提液黏稠得黏液,往其中加入黏液质量40%的麦芽糊精作填充剂,干燥10h,得多穗柯提取物干粉;
(2)酸粒制备:将多穗柯提取物干粉、柠檬酸、酒石酸、木糖醇和阿斯巴甜混合,粉碎,用质量浓度1%聚乙烯吡咯烷酮的无水乙醇润湿,50℃烘干,制粒机制粒,得酸粒;
(3)碱粒制备:将多穗柯提取物干粉、碳酸钠和碳酸氢钠混合,粉碎,50℃烘干,制粒机制粒,得碱粒;
(4)将酸粒、碱粒混合,添加酸粒和碱粒总质量1%的L-HPC,压片机压片,即得成品。
2.根据权利要求1所述的多穗柯提取物泡腾片的制备方法,其特征在于:酸粒中有机酸柠檬酸和酒石酸质量分数为1:1。
3.根据权利要求1所述的多穗柯提取物泡腾片的制备方法,其特征在于:酸粒添加量为成品质量的8%、9%、10%、11%或12%。
4.根据权利要求1所述的多穗柯提取物泡腾片的制备方法,其特征在于:碱粒中碱性物质碳酸钠、碳酸氢钠质量分数1:1。
5.根据权利要求1所述的多穗柯提取物泡腾片的制备方法,其特征在于:碱粒添加量为为成品质量的5%、5.5%、6%、6.5%或7%。
6.根据权利要求1所述的多穗柯提取物泡腾片的制备方法,其特征在于:酒石酸、柠檬酸、碳酸钠、木糖醇、碳酸氢钠、阿斯巴甜、麦芽糊精、抗坏血酸、β-CD、聚乙烯吡咯烷酮、低取代羟丙基纤维素、无水乙醇为食品级。
7.根据权利要求1所述的多穗柯提取物泡腾片的制备方法,其特征在于:步骤(1)中采用旋转蒸发仪将合并滤液浓缩至浸提液黏稠。
8.根据权利要求1-7任一所述方法制备的得到的多穗柯提取物泡腾片。
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