CN108401769A - A kind of cultural method of cercis - Google Patents
A kind of cultural method of cercis Download PDFInfo
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- CN108401769A CN108401769A CN201810280402.XA CN201810280402A CN108401769A CN 108401769 A CN108401769 A CN 108401769A CN 201810280402 A CN201810280402 A CN 201810280402A CN 108401769 A CN108401769 A CN 108401769A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/13—Zeolites
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of cultural method of cercis, which includes following steps：(1) cultivation matrix pre-treatment selects specified viscosity native, plants grass-seed top dressing, ploughs deeply cohesive soil, glass fibre, ceramic grain filter, zeolite powder and the bentonite of the amount of reordering turn over even；(2) nursery paving is built to demarcate cultivation matrix and be limited, the pre-buried built-in pipe at boundary, is connected to setting between different built-in pipes, entrance and exit is exposed outside cultivation matrix；(3) nursery stock transplanting early spring transplants, and selects sapling, digs cave, digs cave；(4) cultivation management applies a nitrogenous fertilizer after the flowers are in blossom every year, and early autumn applies a phosphorus potassium complex fertilizer；To florescence before spring rudiment, the air of 95~97%RH is sent into certain time and speed into built-in pipe；When autumn and rainy season, it is normally opened to open and close valve；The preparation of built-in pipe：It takes PP to manage, opens up multiple through-holes on the side wall and bottom wall of PP pipes, waterproof ventilated membrane is welded at the both ends of through-hole, have the advantages that improve gas permeability.
Description
Technical field
The present invention relates to a kind of cultural methods of cercis.
Background technology
Cercis (scientific name CercischinensisBunge) is pulse family redbud, alias red, purple strain, large bamboo or wicker basket for full item
Tree etc., it is native to China, the provinces such as West of Hubei Province, South Liaoning, Hebei, Shaanxi, Henan, Gansu, Guangdong, Yunnan, Sichuan all
It is distributed.Cercis flower is beautiful, and flower amount is big, and pattern is bright-coloured, when spring important ornamental shrub.It is suitble to greenery patches isolated planting, group planting, or
With other trees mixed plantings, it can also make garden tree or shade tree and be planted with evergreen tree cooperation.
The Chinese patent that application publication number is CN107371962A, application publication number is on November 24th, 2017 discloses one
Kind extends the implantation methods at cercis florescence comprising trimming, fertilising and rush are spent.It can be divided into sand, loam according to the soil texture
And cohesive soil.Sand due between its soil particle hole it is big, capillarity is weak, and water-retaining property and nutrient preserving capability are poor, but its permeability
It is good.Cohesive soil and sand lack macrovoid on the contrary, its heavy texture, tilth is poor between grogs, thus ventilating permeable is poor, both
It is not drought-enduring, also intolerant to waterlogging, but its retain water and nutrients power is strong, resistance to fertilizer, and nutrient is not easy leaching loss, and nutrient content is abundant compared with sand, organic matter
It decomposes slowly, humus easily accumulates.Loam falls between, and is culture base matrix commonly used in the prior art.
Nutritional ingredient in cohesive soil is more, and when using it as culture substrate, poor air permeability, survival rate is low.
Invention content
The object of the present invention is to provide a kind of cultural method of cercis, have the advantages that improve gas permeability.
The present invention above-mentioned technical purpose technical scheme is that：
A kind of cultural method of cercis, includes the following steps：
(1) cultivation matrix pre-treatment
It is 12~15 cohesive soil to select plasticity index, plants grass-seed top dressing on it, and planting density is 25~28g/m2；
Cohesive soil is ploughed deeply, glass fibre, ceramic grain filter, zeolite powder and the bentonite for crushing and crossing 50~80 mesh sieve is added, turns over even；
Total nitrogen content is 0.78~0.84g/kg in the cohesive soil of selection, and content of tatal phosphorus is 0.50~0.55g/kg, hydrolysis
Nitrogen content is 75.5~77.0mg/kg, and quick-acting potassium content is 49.5~52.0mg/kg, and available phosphorus content is 1.90~1.95mg/
Kg, the content of organic matter are 9.20~9.76g/kg, and loam pH is 6.3~6.4；
Glass fibre, ceramic grain filter, zeolite powder and bentonitic dosage be 1.5~1.6,0.6~0.7,0.25~0.28,
5.6~6.0kg/m2；
(2) nursery paving is built
Cultivation matrix is pressed to the specification division limits of 2.0~2.2m × 2.0~2.2m, it is pre-buried at all boundaries
The pre-buried depth of pipe, built-in pipe is 50~55cm；Connection is arranged between different built-in pipes and all nozzles are in addition to entrance and exit
It is all closed processing, keying valve is both provided at entrance and exit and entrance and exit is exposed outside cultivation matrix；
(3) nursery stock transplanting
Early spring transplant, select seedling age 0.2~0.3 year, plant height for 0.50~0.60m plant as sapling, in cultivation base
Each of matter 2.0~2.2m rule centers of a lattice dig cave, and digging acupoint depth is 30~32cm, one plant of seedling of planting trees per cave；
(4) cultivation management
A nitrogenous fertilizer is applied after the flowers are in blossom every year, early autumn applies a phosphorus potassium complex fertilizer；
To florescence before spring rudiment, the keying valve on every 2~3 days entrance and exits for opening built-in pipe, into it
Advertise into the air that relative humidity is 95~97%RH, advertise wind speed be 0.10~0.12m/s and advertise the time be 1.5~
1.8hr；When autumn and rainy season, the keying valve on the entrance and exit of built-in pipe is in normally open；
The preparation method of built-in pipe is：It takes PP to manage, multiple through-holes is opened up on the side wall and bottom wall of PP pipes, in all through-holes
Both ends at be welded with waterproof ventilated membrane.
Further preferably：The waterproof ventilated membrane is multi-layer co-extruded curtain coating comprising the PET polyester films that set gradually,
PET film, PP films, PET film and PET polyester films, PET polyester films, PET film, PP films, PET film and PET polyester films thickness be respectively
0.05~0.06,0.12~0.15,0.08~0.11,0.12~0.15 and 0.05~0.06mm；It is mixed with 5.5 in the PP films~
The glass fibre of 6.0wt%.
Further preferably：Seedling age 0.2~0.3 year, the plant that plant height is 0.50~0.60m pass through tissue cultures, packet
Include following steps：
I, pre-treatment：It collects ripe pod 9~October, takes out seed, cleaning, sterilizing, with 4~6 DEG C of soaked in absolute ethyl alcohol
5.5~6.0hr of seed dries at 25~28 DEG C；
Ii, initial incubation：Seed after pre-treatment is inoculated in A culture mediums, put to 18~20 DEG C and 1500~
It is cultivated 3.5~4.0 days under 16001x；B liquid is added into A culture mediums, is put under 22~24 DEG C and 2000~21001x and cultivates 6.5
~7.0 days, obtain seedling；
A culture mediums are MS+ sucrose 25~27g/l+ agar 6.8~7.0g/l+ sodium chloride 56~60mg/l+ carboxymethyl celluloses
Plain 4.5~4.8mg/l+ of sodium sodium cellulose glycolates 2.5~2.7mg/1, pH are 5.5~6.0；
B liquid include 2.5~2.8wt% of sodium chloride, 0.12~0.15wt% of sodium cellulose glycolate, sodium bicarbonate 4.0~
The base solvent of 4.2wt% and water 22.5~23.2wt%, B liquid is acetone；
By mass, the amount ratio of A culture mediums and B mixed liquors is 1: 0.08~0.10；
Iii, Multiplying culture：Seedling is cut, is transferred in C culture mediums, is put to 22~24 DEG C and 2400~25001x
Culture 15.0~15.5 days；D liquid is added into C culture mediums, is put under 15~16 DEG C and 2400~25001x and cultivates 3.5~4.0
It；
C culture mediums are MS+6- benzyl aminoadenine 3.0~3.2mg/l+, 1.2~1.4mg/l+ of indolebutyric acid methyl α-naphthyl acetates 0.2
1.4~1.5mg/ of~0.3mg/l+ sodium chloride 80~85mg/l+ hydroxypropyl methyl cellulose 2.1~2.2mg/l+ polyacrylamides
l；
D liquid includes 5.1~5.4wt% of sodium chloride, 0.25~0.28wt% of hydroxypropyl methyl cellulose, sodium bicarbonate 3.1
The base solvent of~3.2wt% and water 34.2~34.5wt%, D liquid is ethyl acetate；
By mass, the amount ratio of C culture mediums and D mixed liquors is 1: 0.11~0.12；
Iv, further culture：Seedling after Multiplying culture is cut, is transferred in E culture mediums, put to 8~10 DEG C and
It is cultivated 20.5~21.5 days under 1500~16001x；
E culture mediums be 1/2MS+ indolebutyric acid 0.6~0.8mg/l+, 0.4~0.5mg/l+ of methyl α-naphthyl acetate sodium chloride 125~
44~48mg/l of 130mg/l+ polyacrylamide 1.9~2.0mg/l+ volcanic ash 144~150mg/l+ hydroxypropyl converted starches.
Further preferably：A culture mediums further include PEO-PPO-PEO triblock copolymers 6.5~6.8mg/l and PEO-
3.3~3.5mg/l of PPO diblock copolymers；B liquid further includes 0.5~0.6wt% of lactose；C culture mediums further include diphenol propane
1.1~1.2mg/l of 0.5~0.6mg/l of type epoxy resin and ethylene glycol；D liquid further includes 1.9~2.0wt% of ethylene glycol and the third three
1.5~1.6wt% of alcohol；E culture mediums further include 1.2~1.3mg/l of 4.5~4.7mg/l of ethylene glycol and citric acid.
In conclusion the invention has the advantages that：
Gas permeability is good, and survival rate is high；With preferable acid resistance and salt tolerance.
Specific implementation mode
This specific embodiment is only explanation of the invention, is not limitation of the present invention, people in the art
Member can as needed make the present embodiment the modification of not creative contribution after reading this specification, but as long as at this
It is all protected by Patent Law in the protection domain of invention.
Embodiment 1：A kind of cultural method of cercis, includes the following steps：
(1) cultivation matrix pre-treatment
It is 15 cohesive soil to select plasticity index, plants grass-seed top dressing, planting density 28g/m on it2；Plough deeply viscosity
Soil is added glass fibre, ceramic grain filter, zeolite powder and the bentonite for crushing and crossing 50 mesh sieve, turns over even；
Total nitrogen content is 0.84g/kg, content of tatal phosphorus 0.55g/kg in the cohesive soil of selection, and hydrolysis nitrogen content is
77.0mg/kg, quick-acting potassium content 52.0mg/kg, available phosphorus content 1.95mg/kg, content of organic matter 9.76g/kg, earth
Native pH is 6.3~6.4；
Glass fibre, ceramic grain filter, zeolite powder and bentonitic dosage be 1.6,0.7,0.28,6.0kg/m2；
(2) nursery paving is built
By cultivation matrix by the specification division limits of 2.0m × 2.0m, the pre-buried built-in pipe at all boundaries, built-in pipe
Pre-buried depth is 50cm；Connection is arranged between different built-in pipes and all nozzles are all closed processing, entrance in addition to entrance and exit
It is both provided with keying valve with exit and entrance and exit is exposed outside cultivation matrix；
(3) nursery stock transplanting
Early spring transplant, select seedling age 0.2 year, plant height for 0.50m plant as sapling, in each 2.0m of cultivation matrix
It advises center of a lattice and digs cave, digging acupoint depth is 30cm, one plant of seedling of planting trees per cave；
Seedling age 0.2 year, the plant that plant height is 0.50m are obtained by tissue cultures comprising following steps：
I, pre-treatment：It collects ripe pod 9~October, takes out seed, cleaning, sterilizing, with 4 DEG C of soaked in absolute ethyl alcohol seeds
6.0hr dries at 25 DEG C；
Ii, initial incubation：Seed after pre-treatment is inoculated in A culture mediums, 4.0 are cultivated under putting to 18 DEG C and 16001x
It；B liquid is added into A culture mediums, is put under 22 DEG C and 21001x and cultivates 7.0 days, obtain seedling；
A culture mediums are MS+ sucrose 27g/l+ agar 7.0g/l+ sodium chloride 60mg/l+ sodium carboxymethylcelluloses 4.8mg/l+
Sodium cellulose glycolate 2.7mg/l+PEO-PPO-PEO triblock copolymer 6.8mg/l+PEO-PPO diblock copolymers
3.5mg/l, pH 5.5；
B liquid includes sodium chloride 2.8wt%, sodium cellulose glycolate 0.15wt%, sodium bicarbonate 4.2wt%, water
The base solvent of 23.2wt% and lactose 0.6wt%, B liquid is acetone；
By mass, the amount ratio of A culture mediums and B mixed liquors is 1: 0.10；
Iii, Multiplying culture：Seedling is cut, is transferred in C culture mediums, is cultivated 15.5 days under putting to 22 DEG C and 25001x；
D liquid is added into C culture mediums, is put under 15 DEG C and 25001x and cultivates 4.0 days；
C culture mediums are MS+6- benzyl aminoadenine 3.2mg/l+ indolebutyric acid 1.4mg/l+ methyl α-naphthyl acetate 0.3mg/l+ chlorinations
Sodium 85mg/l+ hydroxypropyl methyl cellulose 2.2mg/l+ polyacrylamide 1.5mg/l+ propane type epoxy resin of di phenolic group 0.6mg/
L and ethylene glycol 1.2mg/l；
D liquid includes sodium chloride 5.4wt%, hydroxypropyl methyl cellulose 0.28wt%, sodium bicarbonate 3.2wt%, water
The base solvent of 34.5wt%, ethylene glycol 2.0wt% and glycerine 1.6wt%, D liquid is ethyl acetate；
By mass, the amount ratio of C culture mediums and D mixed liquors is 1: 0.12；
Iv, further culture：Seedling after Multiplying culture is cut, is transferred in E culture mediums, is put to 8 DEG C and 16001x
Lower culture 21.5 days；
E culture mediums are 1/2MS+ indolebutyric acid 0.8mg/l+ methyl α-naphthyl acetate 0.5mg/l+ sodium chloride 130mg/l+ polyacrylamides
2.0mg/l+ volcanic ash 150mg/l+ hydroxypropyl converted starches 48mg/l+ ethylene glycol 4.7mg/l and citric acid 1.3mg/l；
(4) cultivation management
A nitrogenous fertilizer is applied after the flowers are in blossom every year, early autumn applies a phosphorus potassium complex fertilizer；
To florescence before spring rudiment, the keying valve on the every 3 days entrance and exits for opening built-in pipe is advertised into it
Enter the air that relative humidity is 97%RH, advertises that wind speed is 0.12m/s and to advertise the time be 1.8hr；It is pre-buried when autumn and rainy season
Keying valve on the entrance and exit of pipe is in normally open；
The preparation method of built-in pipe is：It takes PP to manage, multiple through-holes is opened up on the side wall and bottom wall of PP pipes, in all through-holes
Both ends at be welded with waterproof ventilated membrane；
Waterproof ventilated membrane is multi-layer co-extruded curtain coating comprising the PET polyester films that set gradually, PET film, PP films, PET film and
PET polyester films, PET polyester films, PET film, PP films, PET film and PET polyester films thickness be respectively 0.06,0.15,0.11,
0.15 and 0.06mm；The glass fibre of 6.0wt% is mixed in PP films.
Embodiment 2：A kind of cultural method of cercis, includes the following steps：
(1) cultivation matrix pre-treatment
It is 12 cohesive soil to select plasticity index, plants grass-seed top dressing, planting density 25g/m on it2；Plough deeply viscosity
Soil is added glass fibre, ceramic grain filter, zeolite powder and the bentonite for crushing and crossing 80 mesh sieve, turns over even；
Total nitrogen content is 0.78g/kg, content of tatal phosphorus 0.50g/kg in the cohesive soil of selection, and hydrolysis nitrogen content is
75.5mg/kg, quick-acting potassium content 49.5mg/kg, available phosphorus content 1.90mg/kg, content of organic matter 9.20g/kg, earth
Native pH is 6.3；
Glass fibre, ceramic grain filter, zeolite powder and bentonitic dosage be 1.5,0.6,0.25,5.6kg/m2；
(2) nursery paving is built
By cultivation matrix by the specification division limits of 2.2m × 2.2m, the pre-buried built-in pipe at all boundaries, built-in pipe
Pre-buried depth is 55cm；Connection is arranged between different built-in pipes and all nozzles are all closed processing, entrance in addition to entrance and exit
It is both provided with keying valve with exit and entrance and exit is exposed outside cultivation matrix；
(3) nursery stock transplanting
Early spring transplant, select seedling age 0.3 year, plant height for 0.60m plant as sapling, in each 2.2m of cultivation matrix
It advises center of a lattice and digs cave, digging acupoint depth is 32cm, one plant of seedling of planting trees per cave；
Seedling age 0.3 year, the plant that plant height is 0.60m are obtained by tissue cultures comprising following steps：
I, pre-treatment：It collects ripe pod 9~October, takes out seed, cleaning, sterilizing, with 6 DEG C of soaked in absolute ethyl alcohol seeds
5.5hr dries at 28 DEG C；
Ii, initial incubation：Seed after pre-treatment is inoculated in A culture mediums, 3.5 are cultivated under putting to 20 DEG C and 15001x
It；B liquid is added into A culture mediums, is put under 24 DEG C and 20001x and cultivates 6.5 days, obtain seedling；
A culture mediums are MS+ sucrose 25g/l+ agar 6.8g/l+ sodium chloride 56mg/l+ sodium carboxymethylcelluloses 4.5mg/l+
Sodium cellulose glycolate 2.5mg/l+PEO-PPO-PEO triblock copolymer 6.5mg/l+PEO-PPO diblock copolymers
3.3mg/l, pH 6.0；
B liquid includes sodium chloride 2.5wt%, sodium cellulose glycolate 0.12wt%, sodium bicarbonate 4.0wt%, water
The base solvent of 22.5wt% and lactose 0.5wt%, B liquid is acetone；
By mass, the amount ratio of A culture mediums and B mixed liquors is 1: 0.08；
Iii, Multiplying culture：Seedling is cut, is transferred in C culture mediums, is cultivated 15.0 days under putting to 24 DEG C and 2400lx；
D liquid is added into C culture mediums, is put under 16 DEG C and 2400lx and cultivates 3.5 days；
C culture mediums are MS+6- benzyl aminoadenine 3.0mg/l+ indolebutyric acid 1.2mg/l+ methyl α-naphthyl acetate 0.2mg/l+ chlorinations
Sodium 80mg/l+ hydroxypropyl methyl cellulose 2.1mg/l+ polyacrylamide 1.4mg/l+ propane type epoxy resin of di phenolic group 0.5mg/
L and ethylene glycol 1.1mg/l；
D liquid includes sodium chloride 5.1wt%, hydroxypropyl methyl cellulose 0.258wt%, sodium bicarbonate 3.1wt%, water
The base solvent of 34.2wt%, ethylene glycol 1.9wt% and glycerine 1.5wt%, D liquid is ethyl acetate；
By mass, the amount ratio of C culture mediums and D mixed liquors is 1: 0.11；
Iv, further culture：Seedling after Multiplying culture is cut, is transferred in E culture mediums, is put to 10 DEG C and 15001x
Lower culture 20.5 days；
E culture mediums are 1/2MS+ indolebutyric acid 0.6mg/l+ methyl α-naphthyl acetate 0.4mg/l+ sodium chloride 125mg/l+ polyacrylamides
1.9mg/l+ volcanic ash 144mg/l+ hydroxypropyl converted starches 44mg/l+ ethylene glycol 4.5mg/l and citric acid 1.2mg/l；
(4) cultivation management
A nitrogenous fertilizer is applied after the flowers are in blossom every year, early autumn applies a phosphorus potassium complex fertilizer；
To florescence before spring rudiment, the keying valve on the every 2 days entrance and exits for opening built-in pipe is advertised into it
Enter the air that relative humidity is 95%RH, it is 0.10m/ to advertise wind speedsAnd the time is advertised as 1.5hr；It is pre-buried when autumn and rainy season
Keying valve on the entrance and exit of pipe is in normally open；
The preparation method of built-in pipe is：It takes PP to manage, multiple through-holes is opened up on the side wall and bottom wall of PP pipes, in all through-holes
Both ends at be welded with waterproof ventilated membrane；
Waterproof ventilated membrane is multi-layer co-extruded curtain coating comprising the PET polyester films that set gradually, PET film, PP films, PET film and
PET polyester films, PET polyester films, PET film, PP films, PET film and PET polyester films thickness be respectively 0.05,0.12,0.08,
0.12 and 0.05mm；The glass fibre of 5.5wt% is mixed in PP films.
Embodiment 3：A kind of cultural method of cercis, difference from example 1 is that, waterproof ventilated membrane derives from city
It sells, TPU film 0.2mm.
Embodiment 4：A kind of cultural method of cercis, difference from example 1 is that, seedling is purchased from commercially available.
Embodiment 5：A kind of cultural method of cercis, difference from example 1 is that, A culture mediums do not contain PEO-
PPO-PEO triblock copolymers or PEO-PPO diblock copolymers, B liquid do not contain lactose, C culture mediums not type containing diphenol propane
Epoxy resin or ethylene glycol, D liquid do not contain ethylene glycol or glycerine, and E culture mediums do not contain ethylene glycol or citric acid.
Performance test
(1) control group
Control group 1：With reference to the embodiment 1 of CN107371962A.
(2) cultivation test
It is cultivated by test group, 90 days and 360 days survival rates are cultivated in observation.Each test group cultivates 100 plants of plant.Test
The results are shown in Table 1.
The display of table 1 is compared with control group, the survival rate higher of embodiment 1-5.It is found on the built-in pipe of part in research process
Waterproof ventilated membrane and built-in pipe be detached from, the waterproof ventilated membrane on the built-in pipe of part is recessed inwardly by matrix effect, causes to cultivate
Matrix fills built-in pipe.
The cultivation test of table 1
(3) acid resistance is tested
PH to 6.3,5.6,4.7 of matrix, observation are adjusted to cultivate 90 days and 360 days survival rates.Each test group cultivation 100
Strain plant.Test result is as shown in table 2.
The display of table 2, compared with the control group, survival rate highers of the embodiment 1-5 in acid test Tanaka.
2 acid resistance of table is tested
(4) salt tolerance is tested
Adjust the concentration of the sodium chloride of matrix to 0.0,1.0 and 3.5wt%, 90 days and 360 days survival rates are cultivated in observation.Often
A test group cultivates 100 plants of plant.Test result is as shown in table 3.
The display of table 3, compared with the control group, survival rate highers of the embodiment 1-5 in saliferous experimental plot.3 salt tolerance of table is surveyed
Examination
Claims (4)
1. a kind of cultural method of cercis, which is characterized in that include the following steps：
(1) cultivation matrix pre-treatment
It is 12~15 cohesive soil to select plasticity index, plants grass-seed top dressing on it, and planting density is 25~28g/m2；It ploughs deeply
Cohesive soil is added glass fibre, ceramic grain filter, zeolite powder and the bentonite for crushing and crossing 50~80 mesh sieve, turns over even；
Total nitrogen content is 0.78~0.84g/kg in the cohesive soil of selection, and content of tatal phosphorus is 0.50~0.55g/kg, and hydrolyzable nitrogen contains
Amount is 75.5~77.0mg/kg, and quick-acting potassium content is 49.5~52.0mg/kg, and available phosphorus content is 1.90~1.95mg/kg,
The content of organic matter is 9.20~9.76g/kg, and loam pH is 6.3~6.4；
Glass fibre, ceramic grain filter, zeolite powder and bentonitic dosage are 1.5~1.6,0.6~0.7,0.25~0.28,5.6
~6.0kg/m2；
(2) nursery paving is built
By cultivation matrix by the specification division limits of 2.0~2.2m × 2.0~2.2m, the pre-buried built-in pipe at all boundaries, in advance
The pre-buried depth of pipe laying is 50~55cm；Connection is arranged between different built-in pipes and all nozzles are all closed in addition to entrance and exit
It handles, keying valve is both provided at entrance and exit and entrance and exit is exposed outside cultivation matrix；
(3) nursery stock transplanting
Early spring transplant, select seedling age 0.2~0.3 year, plant height for 0.50~0.60m plant as sapling, in cultivation matrix
Each 2.0~2.2m rule center of a lattice digs cave, and digging acupoint depth is 30~32cm, one plant of seedling of planting trees per cave；
(4) cultivation management
A nitrogenous fertilizer is applied after the flowers are in blossom every year, early autumn applies a phosphorus potassium complex fertilizer；
To florescence before spring rudiment, the keying valve on every 2~3 days entrance and exits for opening built-in pipe is advertised into it
Enter the air that relative humidity is 95~97%RH, advertises that wind speed is 0.10~0.12m/s and to advertise the time be 1.5~1.8hr；Autumn
When season and rainy season, the keying valve on the entrance and exit of built-in pipe is in normally open；
The preparation method of built-in pipe is：It takes PP to manage, multiple through-holes is opened up on the side wall and bottom wall of PP pipes, the two of all through-holes
Waterproof ventilated membrane is welded at end.
2. a kind of cultural method of cercis according to claim 1, which is characterized in that the waterproof ventilated membrane is total for multilayer
Squish flow is prolonged comprising PET polyester films, PET film, PP films, PET film and the PET polyester films set gradually, PET polyester films, PET film,
The thickness of PP films, PET film and PET polyester films is respectively 0.05~0.06,0.12~0.15,0.08~0.11,0.12~0.15
With 0.05~0.06mm；The glass fibre of 5.5~6.0wt% is mixed in the PP films.
3. a kind of cultural method of cercis according to claim 2, which is characterized in that seedling age 0.2~0.3 year, plant height are
The plant of 0.50~0.60m passes through tissue cultures comprising following steps：
I, pre-treatment：It collects ripe pod 9~October, takes out seed, cleaning, sterilizing, with 4~6 DEG C of soaked in absolute ethyl alcohol seeds
5.5~6.0hr dries at 25~28 DEG C；
Ii, initial incubation：Seed after pre-treatment is inoculated in A culture mediums, is put to 18~20 DEG C and 1500~1600lx
Culture 3.5~4.0 days；B liquid is added into A culture mediums, is put under 22~24 DEG C and 2000~2100lx and cultivates 6.5~7.0 days,
Obtain seedling；
A culture mediums are MS+ sucrose 25~27g/l+ agar 6.8~7.0g/l+ sodium chloride 56~60mg/l+ sodium carboxymethylcelluloses
4.5~4.8mg/l+ sodium cellulose glycolates 2.5~2.7mg/l, pH are 5.5~6.0；
B liquid include 2.5~2.8wt% of sodium chloride, 0.12~0.15wt% of sodium cellulose glycolate, sodium bicarbonate 4.0~
The base solvent of 4.2wt% and water 22.5~23.2wt%, B liquid is acetone；
By mass, the amount ratio of A culture mediums and B mixed liquors is 1: 0.08~0.10；
Iii, Multiplying culture：Seedling is cut, is transferred in C culture mediums, is cultivated under putting to 22~24 DEG C and 2400~25001x
15.0~15.5 days；D liquid is added into C culture mediums, is put under 15~16 DEG C and 2400~25001x and cultivates 3.5~4.0 days；
C culture mediums be MS+6- benzyl aminoadenine 3.0~3.2mg/l+, 1.2~1.4mg/l+ of indolebutyric acid methyl α-naphthyl acetates 0.2~
1.4~1.5mg/l of 0.3mg/l+ sodium chloride 80~85mg/l+ hydroxypropyl methyl cellulose 2.1~2.2mg/l+ polyacrylamides；
D liquid include 5.1~5.4wt% of sodium chloride, 0.25~0.28wt% of hydroxypropyl methyl cellulose, sodium bicarbonate 3.1~
The base solvent of 3.2wt% and water 34.2~34.5wt%, D liquid is ethyl acetate；
By mass, the amount ratio of C culture mediums and D mixed liquors is 1: 0.11~0.12；
Iv, further culture：Seedling after Multiplying culture is cut, is transferred in E culture mediums, put to 8~10 DEG C and 1500~
It is cultivated 20.5~21.5 days under 1600lx；
E culture mediums are 125~130mg/l of 1/2MS+ indolebutyric acid 0.6~0.8mg/l+ methyl α-naphthyl acetate 0.4~0.5mg/l+ sodium chloride
44~48mg/l of+polyacrylamide 1.9~2.0mg/l+ volcanic ash 144~150mg/l+ hydroxypropyl converted starches.
4. a kind of cultural method of cercis according to claim 3, which is characterized in that A culture mediums further include PEO-PPO-
3.3~3.5mg/l of PEO triblock copolymer 6.5~6.8mg/l and PEO-PPO diblock copolymers；B liquid further includes lactose 0.5
~0.6wt%；C culture mediums further include 1.1~1.2mg/l of 0.5~0.6mg/l of propane type epoxy resin of di phenolic group and ethylene glycol；D
Liquid further includes 1.5~1.6wt% of 1.9~2.0wt% of ethylene glycol and glycerine；E culture mediums further include 4.5~4.7mg/ of ethylene glycol
1.2~1.3mg/l of l and citric acid.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201784223U (en) * | 2010-08-12 | 2011-04-06 | 何永林 | Air-permeable elastic fold non-woven fabric |
| CN103355170A (en) * | 2013-07-24 | 2013-10-23 | 江苏绿苑园林建设有限公司 | Tissue culture method of cerciscanadensis forestpansy |
| CN103814761A (en) * | 2014-03-07 | 2014-05-28 | 陈瑞文 | Delicate agriculture planting creating natural air conveying carbon capturing method and structure thereof |
| CN104782490A (en) * | 2015-05-02 | 2015-07-22 | 冯文杰 | Establishment method of Cercis Canadensis 'Forest Pansy' tissue culture rapid propagation technical system |
-
2018
- 2018-04-01 CN CN201810280402.XA patent/CN108401769A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201784223U (en) * | 2010-08-12 | 2011-04-06 | 何永林 | Air-permeable elastic fold non-woven fabric |
| CN103355170A (en) * | 2013-07-24 | 2013-10-23 | 江苏绿苑园林建设有限公司 | Tissue culture method of cerciscanadensis forestpansy |
| CN103814761A (en) * | 2014-03-07 | 2014-05-28 | 陈瑞文 | Delicate agriculture planting creating natural air conveying carbon capturing method and structure thereof |
| CN104782490A (en) * | 2015-05-02 | 2015-07-22 | 冯文杰 | Establishment method of Cercis Canadensis 'Forest Pansy' tissue culture rapid propagation technical system |
Non-Patent Citations (2)
| Title |
|---|
| 石进朝等: "《彩色苗木繁育技术》", 30 April 2014, 中国农业大学出版社 * |
| 程广有: "《名优花卉组织培养技术》", 31 January 2001, 科学技术文献出版社 * |
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Application publication date: 20180817 |