CN108329094A - A kind of booth vegetable spraying Se-enriched bio preparation and preparation method thereof - Google Patents
A kind of booth vegetable spraying Se-enriched bio preparation and preparation method thereof Download PDFInfo
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- CN108329094A CN108329094A CN201810174525.5A CN201810174525A CN108329094A CN 108329094 A CN108329094 A CN 108329094A CN 201810174525 A CN201810174525 A CN 201810174525A CN 108329094 A CN108329094 A CN 108329094A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C5/00—Fertilisers containing other nitrates
- C05C5/02—Fertilisers containing other nitrates containing sodium or potassium nitrate
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/02—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing at least one abnormal peptide link
- C07K5/0215—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing at least one abnormal peptide link containing natural amino acids, forming a peptide bond via their side chain functional group, e.g. epsilon-Lys, gamma-Glu
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Abstract
The present invention relates to a kind of booth vegetable spraying Se-enriched bio preparations and preparation method thereof.The nutrient solution is digested as the source of selenium element by crushing using biomass straw waste material as raw material, centrifugation, obtains biomass straw waste disposal liquid, then with mineral salt, amino acid, urea, folic acid, composite growth regulator, oxidized form of glutathione, deionized water are mixed in a certain ratio, mixed solution is stirred using magnetic stirring apparatus, then through centrifugation, takes supernatant, it is finally dialysed with NF membrane, obtains filtered Selenium-rich nutrient solution, it is spray-dried to be made.Booth vegetable spraying Se-enriched bio preparation produced by the present invention can not only discharge the natural plant active factor, promote crop root system growth, while the immunity that crops resist pest and disease damage can be improved, and promote crops photosynthesis, improve crop yield and quality.
Description
Technical field
The present invention relates to a kind of biological agents and preparation method thereof, are given birth to selenium-rich more particularly to a kind of booth vegetable spraying
Object preparation and preparation method thereof.
Background technology
Green plants utilizes the CO that luminous energy is absorbed it2And H20 assimilates into organic matter, and the process for releasing oxygen claims
For photosynthesis.It is absorbed there are two the main paths of nutrient, i.e. root and leaf.In addition to root system, crop can also pass through stem
Leaf (especially blade) absorbs nutrient.Foliar fertilizer is the general designation for the fertilizer for providing nutriment for crop by crop leaf.Leaf
Leaven is that nutrient is applied to vegetable leaf surface, and a kind of fertilizer type of base function is played by the absorption of blade, is planted
The blade of object has upper layer and lower layer epidermis, is made of epidermis exquisiteness, there is cuticula and wax on table on the outside of cell, can protect table
Mesophyll cell under skin tissue exercises the functions such as photosynthetic, breathing, is not influenced by the variation of extraneous unfavorable conditions, and blade performance also has
Many small stomatas exercise the function that gas is replaced.Therefore, foliar fertilizer is sprayed on crop leaf, can energy by stomata
Crop nutrient is supplied, and obtains the agricultural product of high yield and high quality.
In recent years, easy to operate due to foliar fertilizer, Foliar Spraying is carried out during vegetable growth increasingly by extensive
Concern, but existing blade fertilizer has improved space in terms of promoting photosynthesis of plant, improving yield.
The present invention provides a kind of vegetables foliage-spray Se-enriched bio preparations, and selenium element is enriched with by biomass waste material,
Through highly purified, the techniques such as compounding are made, while a kind of composite growth regulator being added in nutrient solution, by mutual
Synergistic effect significantly improves absorption of the vegetables to nutrient, promotes photosynthesis, improves the ability that vegetables resist insect pest, carries
High yield of vegetables, while realizing recycling for biological waste.
Invention content
To solve deficiency in the prior art, the present invention provides a kind of booth vegetable spraying Se-enriched bio preparations, should
Se-enriched bio preparation is that Organic Selenium is extracted from biomass waste material, is made through techniques such as highly concentrated, compoundings, can not only discharge day
Right plant active factors improve crops and resist the immunity of pest and disease damage, and can promote crops photosynthesis, improve farming
Produce amount and quality.
The technical solution adopted by the present invention to solve the technical problems is：
A kind of booth vegetable spraying Se-enriched bio preparation, the biomass sources soilless culture Selenium-rich nutrient solution is by following parts by weight
Raw material processing form：150-250 parts of biomass waste material treatment fluid, 15-25 parts of mineral salt, 5-25 parts of amino acid 1, urea
15-25 parts, 5-10 parts of composite growth regulator, 5-10 parts of folic acid, 5-10 parts of oxidized form of glutathione.
The preparation method of the biomass sources soilless culture Selenium-rich nutrient solution is as follows：
1）It by Se-enriched bio matter waste material, is crushed with pulverizer, crosses 80-100 mesh sieve；
2）Superfine grinding is carried out to the waste material after crushing using ball milling instrument, milling time 10-30min, power 10-30HZ are obtained
Raw material is digested, carries out preservation at room temperature；
3）By step 2）In enzymolysis raw material and water 1:3-5（Weight）Mixing, and stir evenly, pH value is adjusted to the left sides 5.6-6.7
The right side is added the cellulase of waste material quality 0.8%-1.2% and is stirred continuously, enzymolysis time 2-4h, 45-55 DEG C of hydrolysis temperature；
4）After enzymolysis, enzymolysis liquid is cooled to 30-50 DEG C in 110-120 DEG C, heating 3-5s enzyme deactivations；
5）Using centrifuge to step 4）Enzymolysis liquid centrifugation after inactivation, centrifuge speed 4000-6000rmp, centrifugation time 5-
10min obtains enzymolysis clear liquid；
6）By step 5）Enzymolysis clear liquid with NF membrane carry out dialysis concentration 1-3 times, the Se-enriched bio matter waste material after being concentrated
Treatment fluid；
7）, by step 6）Obtained Se-enriched bio matter waste disposal liquid and mineral salt, amino acid, urea, folic acid, composite growth
Conditioning agent and oxidized form of glutathione are uniformly mixed by formula ratio, filter off water-insoluble impurity, obtain Se-enriched bio preparation half at
Product；
8）7 will be walked）Obtained Se-enriched bio intermediate formulations are by concentrating, being dried to obtain powdered Se-enriched bio finished dosage form.
Preferably, the Se-enriched bio matter waste material is selenium-rich stalk, selenium-rich bacteria stick, selenium-rich husk waste material.
Preferably, the mineral salt be potassium nitrate, sodium chloride, calcium chloride, magnesium sulfate by weight（0.8-1.2）:
（0.8-1.2）:（0.5-1.2）:（0.5-1.2）Composition.
Preferably, steps are as follows for the preparation method of the oxidized form of glutathione：
1）Reduced glutathione is taken, in 40-45 DEG C of dissolving, the reduced glutathione solution of 50-100g/L is obtained, surveys its pH
Value is A1；
2) with barium hydroxide tune pH to A2,7.0≤A2≤7.2；
3）A certain amount of hydrogen peroxide is added, it is stirring while adding, continue to be stirred to react 0.2-0.5h after adding；
4）After reaction, a certain amount of manganese dioxide is added, stirs 5-10min, then fully filters off manganese dioxide；
5）With sulphur acid for adjusting pH to A3, A1-0.1≤A3≤A1；
6）Barium sulfate precipitate is fully filtered off, spray drying obtains oxidized form of glutathione.
It is furthermore preferred that the dosage of the hydrogen peroxide is 0.4-0.5ml/g glutathione, the mass fraction of hydrogen peroxide is 20-
30%。
Preferably, the composite growth regulator is formed by the Raw material processing of following parts by weight：Digest seaweed residue dry powder
15-25 parts, 5-10 parts of avenabeta glucosan, 0.5-1 parts of polylysine, 1-2 parts of heteroauxin.
It is furthermore preferred that the preparation method of composite growth regulator is as follows：
1）Dry seaweed residue is added in a certain amount of inorganic salt solution, 30min is impregnated；
2）High speed is beaten, and pH to 6.5-6.8 is adjusted after mashing, and the wetting and penetrating agent and 2%- of dry seaweed mass of residue 2%-3% is added
3% complex enzyme zymohydrolysis 30-50min, 45-55 DEG C of hydrolysis temperature, after be heated to 80-90 DEG C of enzyme deactivation 3-5s, be cooled to 30-
50℃；
3）Using centrifuge to step 2）Enzymolysis solution after enzyme deactivation centrifuges, centrifuge speed 4000-6000rmp, centrifugation time 5-
10min takes supernatant, to digest seaweed extracted liquor；
4）Enzymolysis seaweed extracted liquor is subjected to dialysis concentration with NF membrane, the nanofiltration retaining molecular weight is 150-500Da,
Obtain filtered enzymolysis seaweed extracted liquor；
5）Filtered enzymolysis seaweed extracted liquor is further concentrated at 60-80 DEG C of temperature, vacuum degree -0.075-0.095mpa
It is 30-40% to solid content, is then dried to obtain the powdered enzymolysis seaweed that moisture content is 4%-7% in spray dryer again
Residue dry powder；
6）By step 5）Obtained enzymolysis seaweed residue dry powder and avenabeta glucosan, polylysine, heteroauxin, by formula ratio
It is uniformly mixed, is sieved with 80-100 mesh sieve, the growth regulator after being compounded.
Preferably, the wetting and penetrating agent is neopelex, lauryl sodium sulfate, alkylphenol-polyethenoxy
The one or more of mixing of ether, sorbitan fatty acid ester -20, the group of complex enzyme become one kind in cellulase and protease
Or two kinds of mixing.
The beneficial effects of the invention are as follows：The present invention is enriched with selenium element by using biomass waste material, and concentrated purification is another to add
Enter other natural nutritions and inorganic substances, obtains the Se-enriched bio formulation product through compounding, the suction of vegetable nutrient element can be promoted
It receives, sufficient nutrition is provided for the plant growth full stage, while improving the ability that vegetables resist pest and disease damage, promote plant photosynthesis
Effect improves yield of vegetables and quality.
The biological agent of the present invention using when be configured to water the solution of mass fraction 5-10%, be uniformly sprayed on vegetable leaf
Face, continuous sprinkling 5-7 days, can significantly improve vegetable growth performance.
Specific implementation mode
Below by specific embodiment, technical scheme of the present invention will be further explained in detail.
Embodiment 1：
The preparation method of biomass sources soilless culture Selenium-rich nutrient solution is as follows：
1）It by chromium-rich stalk waste material, is crushed with pulverizer, crosses 80 mesh sieve；
2）Superfine grinding is carried out to the waste material after crushing using ball milling instrument, it is former to obtain enzymolysis by milling time 10min, power 10HZ
Material, carries out preservation at room temperature；
3）By step 2）In enzymolysis raw material and water 1:3（Weight）Mixing, and stir evenly, pH value is adjusted to 5.6 or so, is added
The cellulase of waste material quality 0.8% is simultaneously stirred continuously, enzymolysis time 2h, 45 DEG C of hydrolysis temperature；
4）After enzymolysis, enzymolysis liquid is cooled to 30 DEG C in 110 DEG C, heating 3s enzyme deactivations；
5）Using centrifuge to step 4）Enzymolysis liquid centrifugation after inactivation, centrifuge speed 4000rmp, centrifugation time 5min are obtained
Digest clear liquid；
6）By step 5）Enzymolysis clear liquid carry out 1 times of dialysis concentration with NF membrane, at the Se-enriched bio matter waste material after being concentrated
Manage liquid；
7）, by step 6）Obtained Se-enriched bio matter waste disposal liquid and mineral salt, amino acid, urea, folic acid, composite growth
Conditioning agent and oxidized form of glutathione are uniformly mixed by formula ratio, filter off water-insoluble impurity, obtain Se-enriched bio preparation half at
Product；
8）7 will be walked）Obtained Se-enriched bio intermediate formulations are by concentrating, being dried to obtain powdered Se-enriched bio finished dosage form.
Specific to the present embodiment, the mineral salt is potassium nitrate, sodium chloride, calcium chloride, magnesium sulfate by weight 0.8:
0.8:0.5:0.5 composition.
In the present embodiment, steps are as follows for the preparation method of the oxidized form of glutathione：
1）Reduced glutathione is taken, in 40 DEG C of dissolvings, obtains the reduced glutathione solution of 50g/L, it is A1 to survey its pH value；
2) with barium hydroxide tune pH to A2=7.0；
3）A certain amount of hydrogen peroxide is added, it is stirring while adding, continue to be stirred to react 0.2h after adding；
4）After reaction, a certain amount of manganese dioxide is added, stirs 5min, then fully filters off manganese dioxide；
5）With sulphur acid for adjusting pH to A3=A1-0.1；
6）Barium sulfate precipitate is fully filtered off, spray drying obtains oxidized form of glutathione.
In the present embodiment, the mass fraction of the hydrogen peroxide is 20%, and dosage is 0.4ml/g glutathione.
In the present embodiment, the composite growth regulator is formed by the Raw material processing of following parts by weight：Digest seaweed residue
15 parts of dry powder, 5 parts of avenabeta glucosan, 0.5 part of polylysine, 1 part of heteroauxin.
In the present embodiment, the preparation method of composite growth regulator is as follows：
1）30g dry seaweed residues are added in water and inorganic salt solution, 30min is impregnated；
2）High speed is beaten, and PH to 6.5 is adjusted after mashing, and 2% wetting and penetrating agent and 2% complex enzyme zymohydrolysis 30min, enzymolysis temperature is added
Degree 45 DEG C, after be heated to 80 DEG C of enzyme deactivation 3s, be cooled to 30 DEG C；
3）Using centrifuge to step 2）Enzymolysis solution after enzyme deactivation centrifuges, and centrifuge speed 4000rmp, centrifugation time 5min take
Supernatant, to digest seaweed extracted liquor；
4）Enzymolysis seaweed extracted liquor is subjected to dialysis concentration with NF membrane, the nanofiltration retaining molecular weight is 150Da, is obtained
Filtered enzymolysis seaweed extracted liquor；
5）Filtered enzymolysis seaweed extracted liquor is further concentrated into solid content at temperature 60 C, vacuum degree -0.075mpa
Content is 30%, is then dried to obtain the powdered enzymolysis seaweed residue dry powder that moisture content is 4% in spray dryer again；
6）By step 5）Obtained enzymolysis seaweed residue dry powder and avenabeta glucosan, polylysine, heteroauxin, by formula ratio
It is uniformly mixed, is sieved with 80 mesh sieve, the growth regulator after being compounded.
Embodiment two：
The preparation method of biomass sources soilless culture Selenium-rich nutrient solution is as follows：
1）It by selenium-rich bacteria stick waste material, is crushed with pulverizer, crosses 90 mesh sieve；
2）Superfine grinding is carried out to the waste material after crushing using ball milling instrument, it is former to obtain enzymolysis by milling time 20min, power 20HZ
Material, carries out preservation at room temperature；
3）By step 2）In enzymolysis raw material and water 1:4（Weight）Mixing, and stir evenly, pH value is adjusted to 6 or so, is added useless
Expect the cellulase of quality 1% and is stirred continuously, enzymolysis time 3h, 55 DEG C of hydrolysis temperature；
4）After enzymolysis, enzymolysis liquid is cooled to 40 DEG C in 115 DEG C, heating 4s enzyme deactivations；
5）Using centrifuge to step 4）Enzymolysis liquid centrifugation after inactivation, centrifuge speed 5000rmp, centrifugation time 7.5min,
Clear liquid must be digested；
6）By step 5）Enzymolysis clear liquid carry out 2 times of dialysis concentration with NF membrane, at the Se-enriched bio matter waste material after being concentrated
Manage liquid；
7）, by step 6）Obtained Se-enriched bio matter waste disposal liquid and mineral salt, amino acid, urea, folic acid, composite growth
Conditioning agent and oxidized form of glutathione are uniformly mixed by formula ratio, filter off water-insoluble impurity, obtain Se-enriched bio preparation half at
Product；
8）7 will be walked）Obtained Se-enriched bio intermediate formulations are by concentrating, being dried to obtain powdered Se-enriched bio finished dosage form.
In the present embodiment, the mineral salt is potassium nitrate, sodium chloride, calcium chloride, magnesium sulfate by weight 1:1:0.8:
0.8 composition.
In the present embodiment, steps are as follows for the preparation method of the oxidized form of glutathione：
1）Reduced glutathione is taken, in 42 DEG C of dissolvings, obtains the reduced glutathione solution of 75g/L, it is A1 to survey its pH value；
2) with barium hydroxide tune pH to A2=7.1；
3）A certain amount of hydrogen peroxide is added, it is stirring while adding, continue to be stirred to react 0.35h after adding；
4）After reaction, a certain amount of manganese dioxide is added, stirs 7.5min, then fully filters off manganese dioxide；
5）With sulphur acid for adjusting pH to A3=A1-0.1；
6）Barium sulfate precipitate is fully filtered off, spray drying obtains oxidized form of glutathione.
In the present embodiment, the mass fraction of the hydrogen peroxide is 25%, and dosage is 0.45ml/g glutathione.
In the present embodiment, the composite growth regulator is formed by the Raw material processing of following parts by weight：Digest seaweed residue
20 parts of dry powder, 8 parts of avenabeta glucosan, 0.75 part of polylysine, 1.5 parts of heteroauxin.
In the present embodiment, the preparation method of composite growth regulator is as follows：
1）Dry seaweed residue is added in water and inorganic salt solution, 30min is impregnated；
2）High speed is beaten, and PH to 6 is adjusted after mashing, 2.5% wetting and penetrating agent and 2.5% complex enzyme zymohydrolysis 40min is added, and is digested
Temperature 50 C, after be heated to 85 DEG C of enzyme deactivation 3.5s, be cooled to 40 DEG C；
3）Using centrifuge to step 2）Enzymolysis solution after enzyme deactivation centrifuges, and centrifuge speed 5000rmp, centrifugation time 8min take
Supernatant, to digest seaweed extracted liquor；
4）Enzymolysis seaweed extracted liquor is subjected to dialysis concentration with NF membrane, the nanofiltration retaining molecular weight is 300Da, is obtained
Filtered enzymolysis seaweed extracted liquor；
5）Filtered enzymolysis seaweed extracted liquor is further concentrated into solid content at temperature 70 C, vacuum degree 0.015mpa to contain
Amount is 35%, is then dried to obtain the powdered enzymolysis seaweed residue dry powder that moisture content is 6% in spray dryer again；
6）By step 5）Obtained enzymolysis seaweed residue dry powder and avenabeta glucosan, polylysine, heteroauxin, by formula ratio
It is uniformly mixed, is sieved with 90 mesh sieve, the growth regulator after being compounded.
Embodiment 3：
The preparation method of biomass sources soilless culture Selenium-rich nutrient solution is as follows：
1）By selenium-rich husk waste material, is crushed, sieved with 100 mesh sieve with pulverizer；
2）Superfine grinding is carried out to the waste material after crushing using ball milling instrument, it is former to obtain enzymolysis by milling time 30min, power 30HZ
Material, carries out preservation at room temperature；
3）By step（2）In enzymolysis raw material and water 1:5（Weight）Mixing, and stir evenly, pH value is adjusted to 6.7 or so, is added
Enter the cellulase of waste material quality 1.2% and is stirred continuously, enzymolysis time 4h, 55 DEG C of hydrolysis temperature；
4）After enzymolysis, enzymolysis liquid is cooled to 50 DEG C in 120 DEG C, heating 5s enzyme deactivations；
5）Using centrifuge to step 4）Enzymolysis liquid centrifugation after inactivation, centrifuge speed 6000rmp, centrifugation time 10min are obtained
Digest clear liquid；
6）By step 5）Enzymolysis clear liquid carry out 3 times of dialysis concentration with NF membrane, at the Se-enriched bio matter waste material after being concentrated
Manage liquid；
7）, by step 6）Obtained Se-enriched bio matter waste disposal liquid and mineral salt, amino acid, urea, folic acid, composite growth
Conditioning agent and oxidized form of glutathione are uniformly mixed by formula ratio, filter off water-insoluble impurity, obtain Se-enriched bio preparation half at
Product；
8）7 will be walked）Obtained Se-enriched bio intermediate formulations are by concentrating, being dried to obtain powdered Se-enriched bio finished dosage form.
In the present embodiment, the mineral salt is potassium nitrate, sodium chloride, calcium chloride, magnesium sulfate by weight 1.2:1.2:
1.2:1.2 composition.
In the present embodiment, steps are as follows for the preparation method of the oxidized form of glutathione：
1）Reduced glutathione is taken, in 45 DEG C of dissolvings, obtains the reduced glutathione solution of 100g/L, surveying its pH value is
A1；
2) with barium hydroxide tune pH to A2=7.2；
3）A certain amount of hydrogen peroxide is added, it is stirring while adding, continue to be stirred to react 0.5h after adding；
4）After reaction, a certain amount of manganese dioxide is added, stirs 10min, then fully filters off manganese dioxide；
5）With sulphur acid for adjusting pH to A3=A1；
6）Barium sulfate precipitate is fully filtered off, spray drying obtains oxidized form of glutathione.
In the present embodiment, the mass fraction of the hydrogen peroxide is 30%, and dosage is 0.5ml/g glutathione.
In the present embodiment, the composite growth regulator is formed by the Raw material processing of following parts by weight：Digest seaweed residue
25 parts of dry powder, 10 parts of avenabeta glucosan, 1 part of polylysine, 2 parts of heteroauxin.
In the present embodiment, the preparation method of composite growth regulator is as follows：
1）Dry seaweed residue is added in water and inorganic salt solution, 30min is impregnated；
2）High speed is beaten, and PH to 6.8 is adjusted after mashing, 3% alkyl phenol polyoxyethylene ether and 3% amylase and protease is added
Mixture digest 50min, 55 DEG C of hydrolysis temperature, after be heated to 90 DEG C of enzyme deactivation 5s, be cooled to 50 DEG C；
3）Using centrifuge to step 2）Enzymolysis solution after enzyme deactivation centrifuges, and centrifuge speed 6000rmp, centrifugation time 10min take
Supernatant, to digest seaweed extracted liquor；
4）Enzymolysis seaweed extracted liquor is subjected to dialysis concentration with NF membrane, the nanofiltration retaining molecular weight is 500Da, is obtained
Filtered enzymolysis seaweed extracted liquor；
5）Filtered enzymolysis seaweed extracted liquor is further concentrated into solid content at 80 DEG C of temperature, vacuum degree 0.095mpa to contain
Amount is 40%, is then dried to obtain the powdered enzymolysis seaweed residue dry powder that moisture content is 7% in spray dryer again；
6）By step 5）Obtained enzymolysis seaweed residue dry powder and avenabeta glucosan, polylysine, heteroauxin, by formula ratio
It is uniformly mixed, is sieved with 100 mesh sieve, the growth regulator after being compounded.
The present invention provides a kind of vegetables foliage-spray Se-enriched bio preparations, and selenium element is enriched with by biomass waste material,
Through highly purified, the techniques such as compounding are made, while a kind of composite growth regulator being added in nutrient solution, by mutual
Synergistic effect significantly improves absorption of the vegetables to nutrient, promotes photosynthesis, improves the ability that vegetables resist insect pest, carries
High yield of vegetables, while realizing recycling for biological waste.
Above-mentioned embodiment is only a preferred solution of the present invention, not the present invention is made in any form
Limitation, on the premise of not exceeding the technical scheme recorded in the claims also other variations and modifications.
Claims (8)
1. a kind of booth vegetable spraying Se-enriched bio preparation, which is characterized in that the booth vegetable spraying Se-enriched bio system
Agent is formed by the Raw material processing of following parts by weight：150-250 parts of Se-enriched bio matter waste disposal liquid, 15-25 parts of mineral salt, ammonia
15-25 parts of base acid, 15-25 parts of urea, 5-10 parts of composite growth regulator, 5-10 parts of oxidized form of glutathione, the greenhouse vegetable
The preparation method following steps of dish spraying Se-enriched bio preparation：
S1, by Se-enriched bio matter waste material, crushed with pulverizer, cross 80-100 mesh sieve；
S2, superfine grinding is carried out to the waste material after crushing using ball milling instrument, milling time 10-30min, power 10-30HZ are obtained
Raw material is digested, carries out preservation at room temperature；
S3, by the enzymolysis raw material and water in mass ratio 1 in step S2:3-5 is mixed, and is stirred evenly, and adjusts pH value to 5.6-
6.7, the cellulase of waste material quality 0.8%-1.2% is added and is stirred continuously, enzymolysis time 2-4h, 45-55 DEG C of hydrolysis temperature；
After S4, enzymolysis, enzymolysis liquid heats 3-5s enzyme deactivations, is cooled to 30-50 DEG C in 110-120 DEG C；
S5, the enzymolysis liquid after step S4 inactivations is centrifuged using centrifuge, centrifuge speed 4000-6000rmp, centrifugation time 5-
10min obtains enzymolysis clear liquid；
S6, the enzymolysis clear liquid of step S5 is subjected to 1-3 times of dialysis concentration, the Se-enriched bio matter waste material after being concentrated with NF membrane
Treatment fluid；
S7, by the obtained Se-enriched bio matter waste disposal liquid of step S6 and mineral salt, amino acid, urea, folic acid, composite growth
Conditioning agent and oxidized form of glutathione are uniformly mixed by formula ratio, filter off water-insoluble impurity, obtain Se-enriched bio preparation half at
Product；
8）7 will be walked）Obtained Se-enriched bio intermediate formulations are by concentrating, being dried to obtain powdered Se-enriched bio finished dosage form.
2. a kind of preparation method of booth vegetable spraying Se-enriched bio preparation as described in claim 1, it is characterised in that：Institute
It is selenium-rich stalk, selenium-rich bacteria stick and selenium-rich husk waste material to state Se-enriched bio matter waste material.
3. a kind of preparation method of booth vegetable spraying Se-enriched bio preparation as described in claim 1, which is characterized in that institute
State mineral salt be potassium nitrate, sodium chloride, calcium chloride, magnesium sulfate by weight（0.8-1.2）:（0.8-1.2）:（0.5-1.2）:
（0.5-1.2）Composition.
4. a kind of preparation method of booth vegetable spraying Se-enriched bio preparation as described in claim 1, which is characterized in that institute
Stating the preparation method of oxidized form of glutathione, steps are as follows：
S1, reduced glutathione is taken, in 40-45 DEG C of dissolving, obtains the reduced glutathione solution of 50-100g/L, survey it
PH value is A1；
S2, with barium hydroxide tune pH to A2,7.0≤A2≤7.2；
S3, a certain amount of hydrogen peroxide is added, it is stirring while adding, continue to be stirred to react 0.2-0.5h after adding；
A certain amount of manganese dioxide is added in S4, after reaction, stirs 5-10min, then fully filters off manganese dioxide；
S5, with sulphur acid for adjusting pH to A3, A1-0.1≤A3≤A1；
S6, barium sulfate precipitate is fully filtered off, be spray-dried, obtain oxidized form of glutathione.
5. a kind of preparation method of booth vegetable spraying Se-enriched bio preparation as claimed in claim 5, which is characterized in that institute
The mass fraction for stating hydrogen peroxide is 20-30%, and dosage is 0.4-0.5ml/g glutathione.
6. a kind of preparation method of booth vegetable spraying Se-enriched bio preparation as described in claim 1, which is characterized in that institute
Composite growth regulator is stated to be formed by the Raw material processing of following parts by weight：15-25 parts of seaweed residue dry powder is digested, oat β-Portugal is poly-
It is 5-10 parts sugared, 0.5-1 parts of polylysine, 1-2 parts of heteroauxin.
7. a kind of preparation method of booth vegetable spraying Se-enriched bio preparation as claimed in claim 7, which is characterized in that institute
It is as follows to state composite growth regulator preparation method：
S1, dry seaweed residue is added in a certain amount of inorganic salt solution, impregnates 30-40min；
S2, high speed are beaten, and adjust pH to 6.5-6.8 after mashing, the wetting and penetrating agent of addition dry seaweed mass of residue 2%-3% and
The complex enzyme zymohydrolysis 30-50min of 2%-3%, 45-55 DEG C of hydrolysis temperature, after be heated to 80-90 DEG C of enzyme deactivation 3-5s, be cooled to
30-50℃；
S3, step S2 enzymolysis solution after enzyme deactivation is centrifuged using centrifuge, centrifuge speed 4000-6000rmp, centrifugation time 5-
10min takes supernatant, to digest seaweed extracted liquor；
S4, enzymolysis seaweed extracted liquor is subjected to dialysis concentration with NF membrane, the nanofiltration retaining molecular weight is 150-
500Da obtains filtered enzymolysis seaweed extracted liquor；
S5, by filtered enzymolysis seaweed extracted liquor at 60-80 DEG C of temperature, vacuum degree -0.075-0.095mpa further it is dense
It is 30-40% to be reduced to solid content, is then dried to obtain the powdered enzymolysis sea that moisture content is 4%-7% in spray dryer again
Algae residue dry powder；
S6, by the obtained enzymolysis seaweed residue dry powder of step S5 and avenabeta glucosan, polylysine, heteroauxin presses formula ratio
It is uniformly mixed, is sieved with 80-100 mesh sieve, obtains composite growth regulator.
8. a kind of preparation method of booth vegetable spraying Se-enriched bio preparation as claimed in claim 8, which is characterized in that institute
It is neopelex, lauryl sodium sulfate, alkyl phenol polyoxyethylene ether, sorbitan fatty to state wetting and penetrating agent
The one or more of mixing of acid esters -20, the group of complex enzyme become arbitrary two kinds or three in cellulase, amylase and protease
The mixing of kind.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113860963A (en) * | 2021-09-14 | 2021-12-31 | 湖北国硒科技发展有限公司 | Organic selenium-rich nutrient solution and preparation method and application thereof |
| CN114249619A (en) * | 2022-01-20 | 2022-03-29 | 黑龙江省昊千生物科技有限公司 | Foliar application fertilizer for rapidly increasing selenium content of crops |
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| CA2318702A1 (en) * | 1999-09-17 | 2001-03-17 | Tamie Nasu | Fertilizer with a chelate element |
| CN101580852A (en) * | 2009-05-18 | 2009-11-18 | 汤洁 | Method for preparing algae essence by using enzymatic hydrolysis method |
| CN105110972A (en) * | 2015-09-18 | 2015-12-02 | 吴限 | Organic compound fertilizer special for cucumber rich in selenium and glutathione and preparation method of organic compound fertilize |
| CN105541432A (en) * | 2015-12-22 | 2016-05-04 | 高畅 | Edible fungus culture substrate rich in glutathione and selenium |
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| CA2318702A1 (en) * | 1999-09-17 | 2001-03-17 | Tamie Nasu | Fertilizer with a chelate element |
| CN101580852A (en) * | 2009-05-18 | 2009-11-18 | 汤洁 | Method for preparing algae essence by using enzymatic hydrolysis method |
| CN105110972A (en) * | 2015-09-18 | 2015-12-02 | 吴限 | Organic compound fertilizer special for cucumber rich in selenium and glutathione and preparation method of organic compound fertilize |
| CN105541432A (en) * | 2015-12-22 | 2016-05-04 | 高畅 | Edible fungus culture substrate rich in glutathione and selenium |
Cited By (2)
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| CN113860963A (en) * | 2021-09-14 | 2021-12-31 | 湖北国硒科技发展有限公司 | Organic selenium-rich nutrient solution and preparation method and application thereof |
| CN114249619A (en) * | 2022-01-20 | 2022-03-29 | 黑龙江省昊千生物科技有限公司 | Foliar application fertilizer for rapidly increasing selenium content of crops |
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