CN108300772A - 用于靶核酸序列检测的多重淬灭荧光探针及方法 - Google Patents
用于靶核酸序列检测的多重淬灭荧光探针及方法 Download PDFInfo
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- CN108300772A CN108300772A CN201810074770.9A CN201810074770A CN108300772A CN 108300772 A CN108300772 A CN 108300772A CN 201810074770 A CN201810074770 A CN 201810074770A CN 108300772 A CN108300772 A CN 108300772A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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CN2017105874443 | 2017-07-18 | ||
CN201710587444.3A CN107236815A (zh) | 2017-07-18 | 2017-07-18 | 用于靶核酸序列检测的多重淬灭荧光探针及方法 |
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CN108300772A true CN108300772A (zh) | 2018-07-20 |
CN108300772B CN108300772B (zh) | 2020-10-23 |
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CN201710587444.3A Withdrawn CN107236815A (zh) | 2017-07-18 | 2017-07-18 | 用于靶核酸序列检测的多重淬灭荧光探针及方法 |
CN201810074770.9A Active CN108300772B (zh) | 2017-07-18 | 2018-01-25 | 用于靶核酸序列检测的多重淬灭荧光探针及方法 |
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CN201710587444.3A Withdrawn CN107236815A (zh) | 2017-07-18 | 2017-07-18 | 用于靶核酸序列检测的多重淬灭荧光探针及方法 |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111534576A (zh) * | 2020-07-08 | 2020-08-14 | 圣湘生物科技股份有限公司 | 用于荧光定量pcr的方法、组合物、试剂盒及其用途 |
CN113846187A (zh) * | 2021-10-24 | 2021-12-28 | 北京宏微特斯生物科技有限公司 | 利用荧光探针熔解曲线检测待测目标核酸序列的方法及其试剂盒 |
CN114134219A (zh) * | 2021-12-14 | 2022-03-04 | 广州市金圻睿生物科技有限责任公司 | 一种多重核酸检测系统及其制备方法与应用 |
RU2808238C1 (ru) * | 2020-07-08 | 2023-11-28 | Сэншур Байотек Инк. | Способ, композиция и набор для флуоресцентной количественной пцр и их применение |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112795473A (zh) * | 2021-02-04 | 2021-05-14 | 杭州遂曾生物技术有限公司 | 一种淋病奈瑟菌一体化核酸检测卡盒 |
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CN103866037A (zh) * | 2011-01-11 | 2014-06-18 | Seegene株式会社 | 借助于探测和标记的寡核苷酸切割以及延长试验的靶核酸序列检测 |
CN104245959A (zh) * | 2012-03-05 | 2014-12-24 | Seegene株式会社 | 基于探测和标记寡核苷酸切割及延伸试验的从靶核酸序列的核苷酸变异检测 |
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2017
- 2017-07-18 CN CN201710587444.3A patent/CN107236815A/zh not_active Withdrawn
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2018
- 2018-01-25 CN CN201810074770.9A patent/CN108300772B/zh active Active
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US8946404B2 (en) * | 2000-05-09 | 2015-02-03 | Biosearch Technologies, Inc. | Dark quenchers for donor-acceptor energy transfer |
CN1348096A (zh) * | 2000-10-10 | 2002-05-08 | 栾国彦 | 一种均相特异性检测核酸的探针及应用方法 |
JP2012060901A (ja) * | 2010-09-14 | 2012-03-29 | Nagoya Univ | オリゴヌクレオチド及びその利用 |
CN103866037A (zh) * | 2011-01-11 | 2014-06-18 | Seegene株式会社 | 借助于探测和标记的寡核苷酸切割以及延长试验的靶核酸序列检测 |
CN102242211A (zh) * | 2011-07-08 | 2011-11-16 | 无锡锐奇基因生物科技有限公司 | 检测突变核酸序列的方法及试剂盒 |
CN104245959A (zh) * | 2012-03-05 | 2014-12-24 | Seegene株式会社 | 基于探测和标记寡核苷酸切割及延伸试验的从靶核酸序列的核苷酸变异检测 |
CN104903465A (zh) * | 2012-12-27 | 2015-09-09 | Seegene株式会社 | 基于寡核苷酸切割及延伸-依赖性非-杂交探测的靶核酸序列的检测 |
WO2016168750A1 (en) * | 2015-04-15 | 2016-10-20 | Biosearch Technologies, Inc. | Dual quencher probes |
CN104962608A (zh) * | 2015-05-28 | 2015-10-07 | 广东省生态环境与土壤研究所 | 一种基于双淬灭基团核酸自组装技术的双酚a检测方法及检测试剂盒 |
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JUEWEN LIU等: "Improving Fluorescent DNAzyme Biosensors by Combining Inter- and Intramolecular Quenchers", 《ANAL. CHEM.》 * |
M. EAQUB ALI等: "Analysis of Pork Adulteration in Commercial Burgers Targeting Porcine- Specific Mitoch ondrial Cytochrome B Gene by TaqMan Probe Real-Time Polymera se Chain Reaction", 《FOOD ANAL. METHODS》 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111534576A (zh) * | 2020-07-08 | 2020-08-14 | 圣湘生物科技股份有限公司 | 用于荧光定量pcr的方法、组合物、试剂盒及其用途 |
WO2022007224A1 (zh) * | 2020-07-08 | 2022-01-13 | 圣湘生物科技股份有限公司 | 用于荧光定量pcr的方法、组合物、试剂盒及其用途 |
RU2808238C1 (ru) * | 2020-07-08 | 2023-11-28 | Сэншур Байотек Инк. | Способ, композиция и набор для флуоресцентной количественной пцр и их применение |
CN113846187A (zh) * | 2021-10-24 | 2021-12-28 | 北京宏微特斯生物科技有限公司 | 利用荧光探针熔解曲线检测待测目标核酸序列的方法及其试剂盒 |
CN113846187B (zh) * | 2021-10-24 | 2023-07-18 | 北京宏微特斯生物科技有限公司 | 利用荧光探针熔解曲线检测待测目标核酸序列的方法及其试剂盒 |
CN114134219A (zh) * | 2021-12-14 | 2022-03-04 | 广州市金圻睿生物科技有限责任公司 | 一种多重核酸检测系统及其制备方法与应用 |
Also Published As
Publication number | Publication date |
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CN107236815A (zh) | 2017-10-10 |
CN108300772B (zh) | 2020-10-23 |
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Effective date of registration: 20190410 Address after: The first floor of No. 4 workshop of Wudangshan Road Medical Devices Industrial Park, Zhanggong District, Ganzhou City, Jiangxi Province, 341000 Applicant after: Jiangxi Xinxing Medical Technology Co., Ltd. Applicant after: Affiliated hospital of Gannan Medical College first Applicant after: Wuhan Jixi Biotechnology Co.,Ltd. Address before: 341000 A-102, First Floor of Building A, Small Business Incubation Base, Ganzhou Development Zone, Ganzhou City, Jiangxi Province Applicant before: Jiangxi Xian Jujingxin medicine bioengineering Science and Technology Ltd. Applicant before: Affiliated hospital of Gannan Medical College first Applicant before: Wuhan Jixi Biotechnology Co.,Ltd. |
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