CN108291189A - 在具有降低的clr2活性的丝状真菌中生产蛋白质的方法 - Google Patents

在具有降低的clr2活性的丝状真菌中生产蛋白质的方法 Download PDF

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Publication number
CN108291189A
CN108291189A CN201680069639.2A CN201680069639A CN108291189A CN 108291189 A CN108291189 A CN 108291189A CN 201680069639 A CN201680069639 A CN 201680069639A CN 108291189 A CN108291189 A CN 108291189A
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clr2
filamentous fungi
seq
nucleic acid
sequence
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Chinese (zh)
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S·哈夫纳
A·提维森
H·哈特曼
N·伯默尔
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BASF SE
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BASF SE
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/2488—Mannanases
    • C12N9/2491—Beta-mannosidase (3.2.1.25), i.e. mannanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03008—3-Phytase (3.1.3.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01025—Beta-mannosidase (3.2.1.25), i.e. mannanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21063—Oryzin (3.4.21.63)

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  • Life Sciences & Earth Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
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  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201680069639.2A 2015-12-02 2016-12-02 在具有降低的clr2活性的丝状真菌中生产蛋白质的方法 Pending CN108291189A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP15197497 2015-12-02
EP15197497.9 2015-12-02
PCT/EP2016/079526 WO2017093450A1 (en) 2015-12-02 2016-12-02 Method of producing proteins in filamentous fungi with decreased clr2 activity

Publications (1)

Publication Number Publication Date
CN108291189A true CN108291189A (zh) 2018-07-17

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CN201680069639.2A Pending CN108291189A (zh) 2015-12-02 2016-12-02 在具有降低的clr2活性的丝状真菌中生产蛋白质的方法

Country Status (9)

Country Link
US (1) US11299522B2 (en26)
EP (1) EP3384002A1 (en26)
JP (1) JP2019503168A (en26)
KR (1) KR20180084135A (en26)
CN (1) CN108291189A (en26)
BR (1) BR112018009729A8 (en26)
CA (1) CA3004990A1 (en26)
MX (1) MX2018006535A (en26)
WO (1) WO2017093450A1 (en26)

Cited By (1)

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CN113151264A (zh) * 2021-03-11 2021-07-23 上海中溶科技有限公司 一种利用正向调控基因构建高产纤维素酶菌株的方法及应用

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JP2019503168A (ja) 2015-12-02 2019-02-07 ビーエーエスエフ ソシエタス・ヨーロピアＢａｓｆ Ｓｅ Ｃｌｒ２活性が低減した糸状菌においてタンパク質を生産する方法
MX2018006536A (es) 2015-12-02 2018-08-15 Basf Se Metodo para producir proteinas en hongos filamentosos con accion clr1 reducida.
US11738080B2 (en) 2017-08-21 2023-08-29 Dyadic International Inc. Production of flu vaccine in Myceliophthora thermophila
CN110042116A (zh) * 2019-04-30 2019-07-23 贵州大学 一种稻瘟菌生物钟控制基因MoCCG4的重组载体和表达方法
CN112813091A (zh) * 2021-02-03 2021-05-18 山东农业大学 Nat1基因作为筛选标记在卵菌遗传转化中的应用
WO2024107812A1 (en) 2022-11-15 2024-05-23 Basf Corporation Methods of reducing conidiation in cell culture
WO2024132947A1 (en) 2022-12-19 2024-06-27 Basf Se New cellulase promoters for fungal protein production

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Publication number Priority date Publication date Assignee Title
CN113151264A (zh) * 2021-03-11 2021-07-23 上海中溶科技有限公司 一种利用正向调控基因构建高产纤维素酶菌株的方法及应用

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CA3004990A1 (en) 2017-06-08
KR20180084135A (ko) 2018-07-24
MX2018006535A (es) 2018-08-15
WO2017093450A1 (en) 2017-06-08
BR112018009729A2 (pt) 2018-11-21
BR112018009729A8 (pt) 2019-02-26
US11299522B2 (en) 2022-04-12
JP2019503168A (ja) 2019-02-07
EP3384002A1 (en) 2018-10-10
US20200308235A1 (en) 2020-10-01

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