CN108244001A - A kind of method for improving different fissure abdomen fish sperm motility rate and vigor - Google Patents
A kind of method for improving different fissure abdomen fish sperm motility rate and vigor Download PDFInfo
- Publication number
- CN108244001A CN108244001A CN201810128675.2A CN201810128675A CN108244001A CN 108244001 A CN108244001 A CN 108244001A CN 201810128675 A CN201810128675 A CN 201810128675A CN 108244001 A CN108244001 A CN 108244001A
- Authority
- CN
- China
- Prior art keywords
- sperm motility
- rate
- motility rate
- water
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000019100 sperm motility Effects 0.000 title claims abstract description 99
- 238000000034 method Methods 0.000 title claims abstract description 41
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 34
- 210000001015 abdomen Anatomy 0.000 title claims abstract description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 117
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 44
- 230000004720 fertilization Effects 0.000 claims abstract description 44
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 41
- 239000012190 activator Substances 0.000 claims abstract description 39
- 238000002360 preparation method Methods 0.000 claims abstract description 35
- 230000012447 hatching Effects 0.000 claims abstract description 31
- 210000000582 semen Anatomy 0.000 claims abstract description 23
- 239000011780 sodium chloride Substances 0.000 claims abstract description 22
- 235000013601 eggs Nutrition 0.000 claims description 49
- 102000002322 Egg Proteins Human genes 0.000 claims description 23
- 108010000912 Egg Proteins Proteins 0.000 claims description 23
- 210000003746 feather Anatomy 0.000 claims description 23
- 210000004681 ovum Anatomy 0.000 claims description 23
- 239000004744 fabric Substances 0.000 claims description 20
- 210000001647 gastrula Anatomy 0.000 claims description 9
- 238000000386 microscopy Methods 0.000 claims description 8
- 238000012797 qualification Methods 0.000 claims description 8
- 230000013020 embryo development Effects 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
- 230000008961 swelling Effects 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 3
- 210000000287 oocyte Anatomy 0.000 abstract description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 11
- 239000008103 glucose Substances 0.000 description 11
- 230000004913 activation Effects 0.000 description 10
- 238000011835 investigation Methods 0.000 description 9
- 239000011734 sodium Substances 0.000 description 9
- 230000003592 biomimetic effect Effects 0.000 description 6
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 238000007796 conventional method Methods 0.000 description 6
- 241001125816 Coregonus autumnalis Species 0.000 description 5
- 238000012544 monitoring process Methods 0.000 description 5
- 241001677838 Cyprinus carpio nudus Species 0.000 description 4
- 241000784329 Juniperus formosana Species 0.000 description 4
- 230000006378 damage Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 230000013011 mating Effects 0.000 description 4
- 230000004899 motility Effects 0.000 description 4
- 230000000638 stimulation Effects 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000009027 insemination Effects 0.000 description 3
- 241001275867 Schizothorax Species 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 241000252210 Cyprinidae Species 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 210000002455 dental arch Anatomy 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002196 ecbolic effect Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000007115 recruitment Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of methods for improving different fissure abdomen fish sperm motility rate and vigor, include the following steps：(1) hatch the preparation of frame；(2) preparation of fresh semen；(3) preparation of sperm motility rate activator：Sperm motility rate activator is prepared using pure water and NaCl；(4) preparation of sperm motility extension agent：Use pure water, NaCl and C6H12O6Prepare sperm motility extension agent；(5) it inseminates；(6) hatch.The rate of fertilization of the rate of fertilization of different tooth schizothoracin from primitive technology 78.59% or so is increased to more than 97.40% by the present invention, opposite to improve 18.81 percentage points.When Oocyte maturity is good, rate of fertilization can reach more than 99%, and hatching rate is also opposite to improve 23.17 percentage points.
Description
Technical field
The present invention relates to aquaculture field, particularly a kind of method for improving different fissure abdomen fish sperm motility rate and vigor.
Background technology
Different tooth Schizothorax Cypriniformes, Cyprinidae, schizothoracin is sub-, Schizothorax.Local name stick fish, be commonly called as it is European bow fish,
Horizontal four row tooth fish of mouth, secondary schizothoracin and different dental arch fish.Different tooth schizothoracin is China endemic species, is only distributed in the Yarlung Zangbo River
In upstream Heavenly Stems and Earthly Branches stream and attached water body, belong to plateau cold water fishes, be the important indigenous strains in Tibet Yarlung Zangbo River waters,
It is play an important role in the ecosystem of the high and cold fragility in Tibet and one of local main economic fish.
Flourishing due to Xizang road bridge economy in recent years, amount of fishing steeply rises, and leads to the original inhabitants such as different tooth schizothoracin
Main Commercial Fishes quantity drastically declines.
Have been reported that display in June, 2011, Yarlung Zangbo River middle reaches, the Exploitation degree of different fissure abdomen fish Stock resoures is more
Rationally, for the breeding potential of male and female than being respectively 41.8% and 43.6%, population magnitude of recruitment is sufficient, population can kept to stablize
Under the premise of high yield is provided, situation can be said to be optimistic.But after 2011,2012 to 2016 Tibet tourist receipts years
Increase by 136%, huge consumption requirements lead to overfishing, and population quantity falls sharply rapidly.In addition plateau water temperature is low, eats
Object is deficient, and different tooth schizothoracin is slow-growing, sexal maturity is late, concentrates on oviposition breeding in 4 to May, and its population is once by broken
It is bad, it will be difficult to restore, the protection work carried out to its resource is very urgent.Therefore, carry out the artificial propagation work of different tooth schizothoracin
Make, to restore and supplement its Population, be of great significance.And the rate of fertilization in artificial propagation work is improved, ensure
Then it is the key technology in artificial propagation techniques to more fertilized eggs quantity.
Publication number CN104255614A, the invention of entitled " a kind of hard Juniperus formosana Pan naked carp artificial fecundation method of schizothoracin "
Patent, open step are：1st, free of contamination water quality is selected；2nd, using net catch in a manner of collect wild hard Juniperus formosana Pan naked carp after
Standby parent population is raised and train；3rd, selection milter weight is not less than 10g/ from by 1 year wild hard Juniperus formosana Pan naked carp reserve parent fish raised and train
Tail, raun weight not less than 20g/ tails it is physical strong, disease-free and injury-free without deformity hard Juniperus formosana Pan naked carp as breeding parent population into
Row is cultivated；4th, the breeding parent population cultivated by 1 year carries out preparation of hastening parturition；5th, it selects sexually matured parent population and carries out artificial induced spawning；6、
To the parent population artificial insemination after artificial induced spawning；7th, artificial incubation is carried out using flowing water method.
Publication number CN103734044A, the invention of entitled " a kind of induced spawning method of the different tooth schizothoracin of plateau fish " are special
Profit, open step are：The first step selects mature brood fish：Second step selects oxytocic drug type and dosage；Third walks, medicine
Object processing；4th step, medication；5th step, artificial insemination.
The activation of spermatozoa method of traditional schizothoracin is dry method fertilization, i.e., in fertilization process after the mixing of sperm ovum,
Or first mix the spermatozoa preservative fluid of preparation and sperm, then after mixed liquor mix with ovum, directly by breeding pond culture pond
It is mixed into along fertilization chamber wall, is gently agitated for completing activation of spermatozoa, fertilization with feather.Conventional dry fertilization is using cultivation water
It directly activates, the plan ecology that the best activation concentration, glucose to sperm add in time point and activation condition is never made clearly
And detailed specification regulation, the arbitrariness influence of operating personnel are very big.
Publication number CN103734044A, entitled " a kind of Coregonus autumnalis sperm compound activation liquid and its preparation method " carry
A kind of Coregonus autumnalis sperm compound activation liquid is supplied, the activation of spermatozoa liquid is made of glucose and sodium chloride, wherein sodium chloride
There is certain inhibiting effect to saprolegniasis during Coregonus autumnalis fry hatching, glucose swashs Coregonus autumnalis sperm
Active fruit is preferable.
Traditional Coregonus autumnalis sperm compound activation liquid and configuration method are not particularly suited for different tooth schizothoracin, and tradition
Activation of spermatozoa and extension substep are not carried out in method, also without the addition time point of refinement extension agent, it is thus possible to because
The slight change of manual operation brings damage to sperm, ovum, fertilized eggs, reduces hatchability.
In existing method, temporarily not yet to put forth effort to improve in different tooth schizothoracin artificial propagation techniques different tooth schizothoracin by
The method of smart rate.
Invention content
To solve problems of the prior art, the present invention provides it is a kind of improve different fissure abdomen fish sperm motility rate and
The method of vigor.The rate of fertilization of the rate of fertilization of different tooth schizothoracin from primitive technology 78.59% or so can be increased to by the present invention
More than 97.40%, it is opposite to improve 18.81 percentage points.
The technical solution adopted by the present invention is：A kind of method for improving different fissure abdomen fish sperm motility rate and vigor, including with
Lower step：
(1) hatch the preparation of frame：A hatching frame is made, tight smooth being fixed on of screen cloth is hatched on frame；
(2) preparation of fresh semen：Prepare the fresh semen of different tooth schizothoracin；
(3) preparation of sperm motility rate activator：Pure water dress is put in a reservoir after being impregnated 1 hour in culture pond
It takes out, sperm motility rate activator is prepared using pure water and NaCl；
(4) preparation of sperm motility extension agent：Pure water dress is put in a reservoir after being impregnated 1 hour in culture pond
It takes out, uses pure water, NaCl and C6H12O6Prepare sperm motility extension agent；
(5) it inseminates：Microscopy qualification fresh semen and mature egg are mixed in a reservoir, are gently mixed uniformly with feather,
The sperm motility rate activator prepared is poured slowly into along chamber wall immediately, while pouring into using feather edge " ∞ " font at the uniform velocity
5s-7s is stirred, adds the sperm motility extension agent prepared, addition while is at the uniform velocity stirred using feather along " ∞ " font
35s-45s is gently poured into hatching frame after the complete water swelling of fertilized eggs and fertilized eggs is obtained by filtration；
(6) hatch：Fertilized eggs are evenly laid out in frame is hatched, the hatching frame completed is put into the stream of intake-outlet
In pond, and the discharge outlet in flowing water pond is sealed with screen cloth, avoids direct sunlight, during by 40h embryonic developments to primitive gut mid-term,
The dead ovum of white is picked, calculates rate of fertilization.
Preferably, the screen cloth in the step (1) and step (6) is 80 mesh.
Preferably, in the step (3) sperm motility rate activator Na+A concentration of 3.5 ‰ -4.5 ‰.
Preferably, in the step (4) sperm motility extension agent C6H12O6A concentration of 6%-10%, NaCl are a concentration of
3.5‰—4.5‰。
Preferably, the sperm motility rate activator and the weight of sperm motility extension agent added in the step (4) is maturation
5 times of ovum weight.
Preferably, the water temperature of culture pond is 12 DEG C -15 DEG C in the step (3) and step (4).
Preferably, paving ovum density of the fertilized eggs in frame is hatched is 1/cm in the step (6)2- 3/cm2。
Preferably, in the step (6) flowing water pond depth of water 0.6m.
Preferably, the water temperature in flowing water pond is 12 DEG C -15 DEG C in the step (6).
Preferably, the speed of flow is 0.2m/s in the step (6).
The beneficial effects of the invention are as follows：
1st, the rate of fertilization of rate of fertilization from the primitive technology 78.59% or so of different tooth schizothoracin is increased to by the present invention
More than 97.40%, it is opposite to improve 18.81 percentage points.When Oocyte maturity is good, rate of fertilization can reach more than 99%.It incubates
Rate is also opposite to improve 23.17 percentage points.
2nd, the purpose that the present invention impregnates 1h is to make the water temperature of pure water consistent with culture pond water temperature, is reduced when using because of temperature
Stimulation of the difference to fish-egg, sperm, fertilized eggs, the water temperature of culture pond can provide the breeding and life of the most comfortable for different tooth schizothoracin
Long temperature.
3rd, the present invention fresh semen and mature egg of microscopy qualification are mixed in a reservoir, with feather be gently mixed to
Uniformly, the sperm that is attached on ovum can be allowed to combine ovum nearby in the moment of activation, reduce since ovum is found in travelling and
Have lost the energy into ovum.
4th, the present invention adds in extension agent after pouring into sperm motility rate activator stirring 5s-7s, is because by sperm existence point
The energy of sperm starts not enough, to show as locomitivity and begin to decline after analysis discovery 5s-7s, and needing outside help property glycogen is as benefit
It fills, fertilization is completed to obtain lasting locomitivity.
5th, sperm motility extension agent stirring 35s-45s of the present invention is because by sperm the study found that different fissure abdomen milt
The sub really time with fertility only has 35s-45s, until death is no complete fertility after 45s, that is, enables
Hard to bear essence, the fertilized eggs of generation can also develop primitive gut mid-term, but not be it is difficult to which development is exactly developmental deformity, to development backward
The outburst of saprolegniasis leaves condition after 40h to primitive gut mid-term.Effective 35-45s is only stirred, it is long to reduce artificial mixing time
To the damage that fertilized eggs are brought, saprolegniasis incidence is reduced to a certain extent, and then improve hatchability.
6th, the Na of sperm motility rate activator of the present invention+A concentration of 3.5 ‰ -4.5 ‰ be because of natural spawning field investigation hair
The spawning ground of existing different tooth schizothoracin for water quality is pure and fresh, at shoal flowing water of gravel substrate, the water quality Na+ concentration of natural spawning field
It is very low, it is approached with the concentration, sets this concentration that can preferably simulate natural ecological environment, improve the motility rate of sperm.
7th, the C of sperm motility extension agent of the present invention6H12O6A concentration of 6%-10%, NaC l a concentration of 3.5 ‰ -4.5 ‰
It can ensure additionally to give the glucose of the concentration in the case of activating Na+ concentration constant, the glucose of this concentration can allow different
Fissure abdomen milt utilizes, and it is too low or too high cause its dead to be unlikely to concentration.
8th, paving ovum density of the invention can ensure to have cycle stimulation by running water around each fertilized eggs and be unlikely to anoxic,
It has substantially reduced ovum and saprolegniasis probability is obtained due to the downright bad ovum of contact, ensured that each fertilized eggs have enough developmenting spaces.
9th, the water quality that the depth of water of the invention can keep hatchery good and stablize, just right makes entire hatchery
Inlet and outlet water recycled under the conditions of the temperature needed for incubating oosperm, dissolved oxygen, water velocity etc. is met, until membrane.It avoids
The damage that human intervention water quality brings fertilized eggs in hatching process.
10th, it investigates and supervises in natural spawning field for many years when the water temperature in flowing water pond is different tooth schizothoracin mating period in the present invention
Gained is surveyed, the water temperature of different tooth schizothoracin natural breed, reaches biomimetic purpose close under natural conditions.
11st, the water velocity in flowing water pond is for many years obtained by the investigation and monitoring of natural spawning field, close to nature item in the present invention
The flow stimulation flow velocity of different tooth schizothoracin natural breed under part, while fertilized eggs is avoided always to stay in a position for hatching frame,
Realization allows fertilized eggs to move up, and reaches biomimetic purpose.
Specific embodiment
The embodiment of the present invention is described in detail below.
Embodiment 1
A kind of method for improving different fissure abdomen fish sperm motility rate and vigor, includes the following steps：
(1) hatch the preparation of frame：A hatching frame is made, tight smooth being fixed on of screen cloth is hatched on frame；
(2) preparation of fresh semen：Prepare the fresh semen of different tooth schizothoracin；
(3) preparation of sperm motility rate activator：Pure water dress is put in a reservoir after being impregnated 1 hour in culture pond
It takes out, sperm motility rate activator is prepared using pure water and NaCl；
(4) preparation of sperm motility extension agent：Pure water dress is put in a reservoir after being impregnated 1 hour in culture pond
It takes out, uses pure water, NaCl and C6H12O6Prepare sperm motility extension agent；
(5) it inseminates：Microscopy qualification fresh semen and mature egg are mixed in a reservoir, are gently mixed uniformly with feather,
The sperm motility rate activator prepared is poured slowly into along chamber wall immediately, while pouring into using feather edge " ∞ " font at the uniform velocity
6s is stirred, adds the sperm motility extension agent prepared, 40s is at the uniform velocity stirred along " ∞ " font using feather while addition, treats
It is gently poured into after the complete water swelling of fertilized eggs in hatching frame and fertilized eggs is obtained by filtration；
(6) hatch：Fertilized eggs are evenly laid out in frame is hatched, the hatching frame completed is put into the stream of intake-outlet
In pond, and the discharge outlet in flowing water pond is sealed with screen cloth, avoids direct sunlight, during by 40h embryonic developments to primitive gut mid-term,
The dead ovum of white is picked, calculates rate of fertilization.
The rate of fertilization of rate of fertilization from the primitive technology 78.59% of different tooth schizothoracin is increased to 97.40% by the present invention, phase
To improving 18.81 percentage points.When Oocyte maturity is good, rate of fertilization can reach 99%.Hatching rate is also opposite to be improved
23.17 percentage points.
Embodiment 2
A kind of method for improving different fissure abdomen fish sperm motility rate and vigor, includes the following steps：
(1) hatch the preparation of frame：A hatching frame is made, tight smooth being fixed on of screen cloth is hatched on frame；
(2) preparation of fresh semen：Prepare the fresh semen of different tooth schizothoracin；
(3) preparation of sperm motility rate activator：Pure water dress is put in a reservoir after being impregnated 1 hour in culture pond
It takes out, sperm motility rate activator is prepared using pure water and NaCl；
(4) preparation of sperm motility extension agent：Pure water dress is put in a reservoir after being impregnated 1 hour in culture pond
It takes out, uses pure water, NaCl and C6H12O6Prepare sperm motility extension agent；
(5) it inseminates：Microscopy qualification fresh semen and mature egg are mixed in a reservoir, are gently mixed uniformly with feather,
The sperm motility rate activator prepared is poured slowly into along chamber wall immediately, while pouring into using feather edge " ∞ " font at the uniform velocity
5s is stirred, adds the sperm motility extension agent prepared, 35s is at the uniform velocity stirred along " ∞ " font using feather while addition, treats
It is gently poured into after the complete water swelling of fertilized eggs in hatching frame and fertilized eggs is obtained by filtration；
(6) hatch：Fertilized eggs are evenly laid out in frame is hatched, the hatching frame completed is put into the stream of intake-outlet
In pond, and the discharge outlet in flowing water pond is sealed with screen cloth, avoids direct sunlight, during by 40h embryonic developments to primitive gut mid-term,
The dead ovum of white is picked, calculates rate of fertilization.
Screen cloth in the step (1) and step (6) is 80 mesh；The water of culture pond in the step (3) and step (4)
Temperature is 15 DEG C；The sperm motility rate activator and the weight of sperm motility extension agent added in the step (4) is mature egg weight
5 times of amount；The paving ovum density is 1/cm2。
The rate of fertilization of rate of fertilization from the primitive technology 78.59% of different tooth schizothoracin is increased to 97.51% by the present invention, phase
To improving 18.92 percentage points.When Oocyte maturity is good, rate of fertilization can reach 99.1%.Hatching rate is also opposite to be improved
23.27 percentage points.
Embodiment 3
A kind of method for improving different fissure abdomen fish sperm motility rate and vigor, includes the following steps：
(1) hatch the preparation of frame：A hatching frame is made, tight smooth being fixed on of screen cloth is hatched on frame；
(2) preparation of fresh semen：Prepare the fresh semen of different tooth schizothoracin；
(3) preparation of sperm motility rate activator：Pure water dress is put in a reservoir after being impregnated 1 hour in culture pond
It takes out, sperm motility rate activator is prepared using pure water and NaCl；
(4) preparation of sperm motility extension agent：Pure water dress is put in a reservoir after being impregnated 1 hour in culture pond
It takes out, uses pure water, NaCl and C6H12O6Prepare sperm motility extension agent；
(5) it inseminates：Microscopy qualification fresh semen and mature egg are mixed in a reservoir, are gently mixed uniformly with feather,
The sperm motility rate activator prepared is poured slowly into along chamber wall immediately, while pouring into using feather edge " ∞ " font at the uniform velocity
7s is stirred, adds the sperm motility extension agent prepared, 45s is at the uniform velocity stirred along " ∞ " font using feather while addition, treats
It is gently poured into after the complete water swelling of fertilized eggs in hatching frame and fertilized eggs is obtained by filtration；
(6) hatch：Fertilized eggs are evenly laid out in frame is hatched, the hatching frame completed is put into the stream of intake-outlet
In pond, and the discharge outlet in flowing water pond is sealed with screen cloth, avoids direct sunlight, during by 40h embryonic developments to primitive gut mid-term,
The dead ovum of white is picked, calculates rate of fertilization.
Screen cloth in the step (1) and step (6) is 80 mesh；The water of culture pond in the step (3) and step (4)
Temperature is 12 DEG C；The sperm motility rate activator and the weight of sperm motility extension agent added in the step (4) is mature egg weight
5 times of amount；The paving ovum density is 2/cm2。
The rate of fertilization of rate of fertilization from the primitive technology 78.59% of different tooth schizothoracin is increased to 97.62% by the present invention, phase
To improving 19.03 percentage points.When Oocyte maturity is good, rate of fertilization can reach 99.31%.Hatching rate is also opposite to be improved
23.48 percentage points.
Embodiment 4
A kind of method for improving different fissure abdomen fish sperm motility rate and vigor, includes the following steps：
(1) hatch the preparation of frame：A hatching frame is made, tight smooth being fixed on of screen cloth is hatched on frame；
(2) preparation of fresh semen：Prepare the fresh semen of different tooth schizothoracin；
(3) preparation of sperm motility rate activator：Pure water dress is put in a reservoir after being impregnated 1 hour in culture pond
It takes out, sperm motility rate activator is prepared using pure water and NaCl；
(4) preparation of sperm motility extension agent：Pure water dress is put in a reservoir after being impregnated 1 hour in culture pond
It takes out, uses pure water, NaCl and C6H12O6Prepare sperm motility extension agent；
(5) it inseminates：Microscopy qualification fresh semen and mature egg are mixed in a reservoir, are gently mixed uniformly with feather,
The sperm motility rate activator prepared is poured slowly into along chamber wall immediately, while pouring into using feather edge " ∞ " font at the uniform velocity
5s is stirred, adds the sperm motility extension agent prepared, 44s is at the uniform velocity stirred along " ∞ " font using feather while addition, treats
It is gently poured into after the complete water swelling of fertilized eggs in hatching frame and fertilized eggs is obtained by filtration；
(6) hatch：Fertilized eggs are evenly laid out in frame is hatched, the hatching frame completed is put into the stream of intake-outlet
In pond, and the discharge outlet in flowing water pond is sealed with screen cloth, avoids direct sunlight, during by 40h embryonic developments to primitive gut mid-term,
The dead ovum of white is picked, calculates rate of fertilization.
Screen cloth in the step (1) and (6) is 80 mesh；The water temperature of culture pond is in the step (3) and step (4)
13℃；The sperm motility rate activator and the weight of sperm motility extension agent added in the step (4) is mature egg weight
5 times；The paving ovum density is 3/cm2。
The rate of fertilization of rate of fertilization from the primitive technology 78.59% of different tooth schizothoracin is increased to 97.63% by the present invention, phase
To improving 19.04 percentage points.When Oocyte maturity is good, rate of fertilization can reach 99.32%.Hatching rate is also opposite to be improved
23.49 percentage points.
Embodiment 5
A kind of method for improving different fissure abdomen fish sperm motility rate and vigor, includes the following steps：
(1) hatch the preparation of frame：A hatching frame is made, tight smooth being fixed on of screen cloth is hatched on frame；
(2) preparation of fresh semen：Prepare the fresh semen of different tooth schizothoracin；
(3) preparation of sperm motility rate activator：Pure water dress is put in a reservoir after being impregnated 1 hour in culture pond
It takes out, sperm motility rate activator is prepared using pure water and NaCl；
(4) preparation of sperm motility extension agent：Pure water dress is put in a reservoir after being impregnated 1 hour in culture pond
It takes out, uses pure water, NaCl and C6H12O6Prepare sperm motility extension agent；
(5) it inseminates：Microscopy qualification fresh semen and mature egg are mixed in a reservoir, are gently mixed uniformly with feather,
The sperm motility rate activator prepared is poured slowly into along chamber wall immediately, while pouring into using feather edge " ∞ " font at the uniform velocity
7s is stirred, adds the sperm motility extension agent prepared, 36s is at the uniform velocity stirred along " ∞ " font using feather while addition, treats
It is gently poured into after the complete water swelling of fertilized eggs in hatching frame and fertilized eggs is obtained by filtration；
(6) hatch：Fertilized eggs are evenly laid out in frame is hatched, the hatching frame completed is put into the stream of intake-outlet
In pond, and the discharge outlet in flowing water pond is sealed with screen cloth, avoids direct sunlight, during by 40h embryonic developments to primitive gut mid-term,
The dead ovum of white is picked, calculates rate of fertilization.
Screen cloth in the step (1) and step (6) is 80 mesh；The water of culture pond in the step (3) and step (4)
Temperature is 14 DEG C；The sperm motility rate activator and the weight of sperm motility extension agent added in the step (4) is mature egg weight
5 times of amount.
The rate of fertilization of rate of fertilization from the primitive technology 78.59% of different tooth schizothoracin is increased to 97.64% by the present invention, phase
To improving 19.05 percentage points.When Oocyte maturity is good, rate of fertilization can reach 99.32%.Hatching rate is also opposite to be improved
23.49 percentage points.
Embodiment 6
The present embodiment is on the basis of embodiment 2, the Na of sperm motility rate activator in the step (3)+It is a concentration of
3.5‰。
The Na of sperm motility rate activator of the present invention+A concentration of 3.5 ‰ be because the investigation of natural spawning field finds different fissure abdomen
The spawning ground of fish for water quality is pure and fresh, at shoal flowing water of gravel substrate, the water quality Na+ concentration of natural spawning field is very low, dense with this
Degree is close, sets this concentration that can preferably simulate natural ecological environment, improves the motility rate of sperm.
Embodiment 7
The present embodiment is on the basis of embodiment 3, the Na of sperm motility rate activator in the step (3)+It is a concentration of
4.5‰。
The Na of sperm motility rate activator of the present invention+A concentration of 4.5 ‰ be because the investigation of natural spawning field finds different fissure abdomen
The spawning ground of fish for water quality is pure and fresh, at shoal flowing water of gravel substrate, the water quality Na+ concentration of natural spawning field is very low, dense with this
Degree is close, sets this concentration that can preferably simulate natural ecological environment, improves the motility rate of sperm.
Embodiment 8
The present embodiment is on the basis of embodiment 4, the Na of sperm motility rate activator in the step (3)+A concentration of 4 ‰.
The Na of sperm motility rate activator of the present invention+A concentration of 4 ‰ be because the investigation of natural spawning field finds different tooth schizothoracin
Spawning ground for water quality is pure and fresh, at shoal flowing water of gravel substrate, the water quality Na+ concentration of natural spawning field is very low, with the concentration
It is close, it sets this concentration that can preferably simulate natural ecological environment, improves the motility rate of sperm.
Embodiment 9
The present embodiment is on the basis of embodiment 6, the C of sperm motility extension agent in the step (4)6H12O6It is a concentration of
6%th, NaC l a concentration of 3.5 ‰.
The C of sperm motility extension agent of the present invention6H12O6A concentration of 6%, NaC l a concentration of 3.5 ‰ can ensure to activate Na
The glucose of the concentration is additionally given in the case that+concentration is constant, the glucose of this concentration can allow different fissure abdomen milt sharp
With, and it is too low or too high cause its dead to be unlikely to concentration.
Embodiment 10
The present embodiment is on the basis of embodiment 7, the C of sperm motility extension agent in the step (4)6H12O6It is a concentration of
10%th, NaC l a concentration of 4.5 ‰.
The C of sperm motility extension agent of the present invention6H12O6A concentration of 10%, NaCl concentration can ensure to activate Na for 4.5 ‰
The glucose of the concentration is additionally given in the case that+concentration is constant, the glucose of this concentration can allow different fissure abdomen milt sharp
With, and it is too low or too high cause its dead to be unlikely to concentration.
Embodiment 11
The present embodiment is on the basis of embodiment 8, the C of sperm motility extension agent in the step (4)6H12O6It is a concentration of
8%th, NaC l a concentration of 4 ‰.
The C of sperm motility extension agent of the present invention6H12O6A concentration of 8%, NaCl concentration can ensure to activate Na+ dense for 4 ‰
Spend it is constant in the case of additionally give the glucose of the concentration, the glucose of this concentration can allow different fissure abdomen milt to utilize,
It is too low or too high cause its dead to be unlikely to concentration again.
Embodiment 12
The present embodiment is on the basis of embodiment 11, the depth of water 0.6m in flowing water pond in the step (6).
Water quality that the depth of water of the present invention can keep hatchery good and stablize is just right to make entire hatchery
Inlet and outlet water recycles under the conditions of the temperature needed for incubating oosperm, dissolved oxygen, water velocity etc. is met, until membrane.It avoids and incubates
The injury that human intervention water quality brings fertilized eggs during change.
Embodiment 13
The present embodiment is on the basis of embodiment 12, and the water temperature in flowing water pond is 12 DEG C in the step (6).
For many years in natural spawning field investigation and monitoring institute when the water temperature in flowing water pond is different tooth schizothoracin mating period in the present invention
, the water temperature of different tooth schizothoracin natural breed, reaches biomimetic purpose close under natural conditions.
Embodiment 14
The present embodiment is on the basis of embodiment 12, and the water temperature in flowing water pond is 14 DEG C in the step (6).
For many years in natural spawning field investigation and monitoring institute when the water temperature in flowing water pond is different tooth schizothoracin mating period in the present invention
, the water temperature of different tooth schizothoracin natural breed, reaches biomimetic purpose close under natural conditions.
Embodiment 15
The present embodiment is on the basis of embodiment 12, and the water temperature in flowing water pond is 15 DEG C in the step (6).
For many years in natural spawning field investigation and monitoring institute when the water temperature in flowing water pond is different tooth schizothoracin mating period in the present invention
, the water temperature of different tooth schizothoracin natural breed, reaches biomimetic purpose close under natural conditions.
Embodiment 16
The present embodiment is on the basis of embodiment 15, and the speed of flow is 0.2m/s in the step (6).
The water velocity in flowing water pond is for many years obtained by the investigation and monitoring of natural spawning field, close to natural conditions in the present invention
Under different tooth schizothoracin natural breed flow stimulation flow velocity, while fertilized eggs is avoided always to stay in a position of hatching frame, it is real
Now fertilized eggs is allowed to move up, reach biomimetic purpose.
Embodiment 17
Using the rate of fertilization of conventional method and the different tooth schizothoracin of the method for the present invention artificial insemination, hatchability to such as table 1
It is shown.
1 conventional method of table and the method for the present invention rate of fertilization, the comparison of hatchability
By table 1 it is found that the rate of fertilization of the method for the present invention is 97.40%, the rate of fertilization of conventional method is 78.59%, this
The rate of fertilization of inventive method improves 18.81 percentage points than the rate of fertilization of conventional method；The hatchability of the method for the present invention is
84.27%, the hatchability of conventional method is 61.10%, and the hatchability of the method for the present invention is improved than the hatchability of conventional method
23.17 percentage points, it can be seen that the rate of fertilization and hatchability that different tooth schizothoracin can be greatly improved of the method for the present invention.
Embodiment described above only expresses the specific embodiment of the present invention, and description is more specific and detailed, but simultaneously
Cannot the limitation to the scope of the claims of the present invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the present invention's
Protection domain.
Claims (10)
- A kind of 1. method for improving different fissure abdomen fish sperm motility rate and vigor, which is characterized in that include the following steps：(1) hatch the preparation of frame：A hatching frame is made, tight smooth being fixed on of screen cloth is hatched on frame；(2) preparation of fresh semen：Prepare the fresh semen of different tooth schizothoracin；(3) preparation of sperm motility rate activator：Pure water dress is put in a reservoir in culture pond after impregnating 1 hour and taken out, Sperm motility rate activator is prepared using pure water and NaCl；(4) preparation of sperm motility extension agent：Pure water dress is put in a reservoir in culture pond after impregnating 1 hour and taken out, Use pure water, NaCl and C6H12O6Prepare sperm motility extension agent；(5) it inseminates：The fresh semen of microscopy qualification and mature egg are mixed in a reservoir, are gently mixed with feather to uniform, The sperm motility rate activator prepared along chamber wall is poured slowly into immediately, is at the uniform velocity stirred along " ∞ " font using feather while pouring into 5s-7s is mixed, adds the sperm motility extension agent prepared, at the uniform velocity stirs 35s- along " ∞ " font using feather while addition 45s is gently poured into after the complete water swelling of fertilized eggs and is sterilized completely and fertilized eggs are obtained by filtration in the hatching frame bubbled through the water column；(6) hatch：Fertilized eggs are evenly laid out in frame is hatched, the hatching frame completed is put into the flowing water pond of intake-outlet In, and the discharge outlet in flowing water pond is sealed with screen cloth, avoids direct sunlight, during by 40h embryonic developments to primitive gut mid-term, is picked white The dead ovum of color calculates rate of fertilization.
- A kind of 2. method for improving different fissure abdomen fish sperm motility rate and vigor according to claim 1, which is characterized in that institute It is 80 mesh to state the screen cloth in step (1) and step (6).
- A kind of 3. method for improving different fissure abdomen fish sperm motility rate and vigor according to claim 1, which is characterized in that institute State the Na of sperm motility rate activator in step (3)+A concentration of 3.5 ‰ -4.5 ‰.
- A kind of 4. method for improving different fissure abdomen fish sperm motility rate and vigor according to claim 1, which is characterized in that institute State the C of sperm motility extension agent in step (4)6H12O6A concentration of 6%-10%, NaCl concentration are 3.5 ‰ -4.5 ‰.
- A kind of 5. method for improving different fissure abdomen fish sperm motility rate and vigor according to claim 1, which is characterized in that institute State 5 times of the weight of the sperm motility rate activator added in step (4) and sperm motility extension agent for mature egg weight.
- A kind of 6. method for improving different fissure abdomen fish sperm motility rate and vigor according to claim 1, which is characterized in that institute The water temperature for stating culture pond in step (3) and step (4) is 12 DEG C -15 DEG C.
- A kind of 7. method for improving different fissure abdomen fish sperm motility rate and vigor according to claim 1, which is characterized in that institute It is 1/cm to state paving ovum density of the fertilized eggs in frame is hatched in step (6)2- 3/cm2。
- A kind of 8. method for improving different fissure abdomen fish sperm motility rate and vigor according to claim 1, which is characterized in that institute State the depth of water 0.6m in flowing water pond in step (6).
- A kind of 9. method for improving different fissure abdomen fish sperm motility rate and vigor according to claim 1, which is characterized in that institute The water temperature for stating flowing water pond in step (6) is 12 DEG C -15 DEG C.
- 10. a kind of method for improving different fissure abdomen fish sperm motility rate and vigor according to claim 1, which is characterized in that The speed of flow is 0.2m/s in the step (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810128675.2A CN108244001B (en) | 2018-02-08 | 2018-02-08 | Method for improving sperm motility rate and vitality of schizothorax prenanti |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810128675.2A CN108244001B (en) | 2018-02-08 | 2018-02-08 | Method for improving sperm motility rate and vitality of schizothorax prenanti |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108244001A true CN108244001A (en) | 2018-07-06 |
| CN108244001B CN108244001B (en) | 2020-12-11 |
Family
ID=62744291
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810128675.2A Active CN108244001B (en) | 2018-02-08 | 2018-02-08 | Method for improving sperm motility rate and vitality of schizothorax prenanti |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108244001B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130057722A (en) * | 2011-11-24 | 2013-06-03 | 전라남도 | Method for mass producing artificial seed of abudefduf vaigiensis |
| CN104255614A (en) * | 2014-10-13 | 2015-01-07 | 丽江滇江鱼类繁育有限公司 | Artificial propagation method of schizothorax gymnocypris potanini firmispinatus |
| CN105994025A (en) * | 2016-05-11 | 2016-10-12 | 宿松县龙源水产养殖专业合作社 | Practical loach incubation basket |
| CN106359183A (en) * | 2016-08-26 | 2017-02-01 | 新疆赛湖渔业科技开发有限公司 | Coregonus autumnalis sperm compound activation liquid and preparation method thereof |
-
2018
- 2018-02-08 CN CN201810128675.2A patent/CN108244001B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130057722A (en) * | 2011-11-24 | 2013-06-03 | 전라남도 | Method for mass producing artificial seed of abudefduf vaigiensis |
| CN104255614A (en) * | 2014-10-13 | 2015-01-07 | 丽江滇江鱼类繁育有限公司 | Artificial propagation method of schizothorax gymnocypris potanini firmispinatus |
| CN105994025A (en) * | 2016-05-11 | 2016-10-12 | 宿松县龙源水产养殖专业合作社 | Practical loach incubation basket |
| CN106359183A (en) * | 2016-08-26 | 2017-02-01 | 新疆赛湖渔业科技开发有限公司 | Coregonus autumnalis sperm compound activation liquid and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 于清泉: "《鲶形目良种鱼养殖技术》", 30 June 2001, 金盾出版社 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108244001B (en) | 2020-12-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101066046B (en) | Curled-lip mandarin fish and spot and spotted mandarin fish hybridizing propagation process | |
| CN104663547B (en) | A kind of perch carp large-scale artificial propagation method | |
| CN101720698B (en) | Method for distant hybridization of megalobrama amblycephala and erythroculter ilishaeformis | |
| CN101919359B (en) | Method for comprehensively regulating and controlling gonad mature ovulation of cheilinus undulates | |
| CN104904636B (en) | A kind of induced spawning method of little yellow croaker | |
| CN103931525B (en) | The artificial propagation of the naked skin-carp meat in Xinjiang and fry rearing method | |
| CN102077806A (en) | Method for artificial propagation of giant salamanders and cultivation of baby giant salamanders | |
| CN104221952B (en) | A kind of Chalcalbumus chalcoides aralensis artificial fecundation method | |
| CN108967278A (en) | A kind of artificial fecundation method of Scatophagus argus (Linnaeus) | |
| CN104255615B (en) | It is a kind of that soft thorn is naked splits buttocks fish artificial fecundation method | |
| CN104255608B (en) | Artificial spawning method for thick-lipped nude hemibarbus labeo | |
| CN103651220A (en) | Barbodes fuxianensis artificial propagation technology | |
| CN102599094B (en) | Method for artificial incubation of Percocypris pingi pingi | |
| CN112167118A (en) | Artificial propagation method of zier whitefish | |
| CN105265362A (en) | Cross breeding method for improving growth traits of slender mandarinfish | |
| CN103688880A (en) | Hybrid breeding method with siniperca chuatsi as male parent and siniperca scherzeri as female parent | |
| CN106879515A (en) | A kind of viscidity-removing method of body graining embryonated egg high | |
| CN106376500B (en) | The thin squama schizothoracin artificial fecundation of Yalongjiang River releases method | |
| CN106804477A (en) | A kind of artificial fecundation method of Pelteobagrus fulvidraco | |
| CN108244001A (en) | A kind of method for improving different fissure abdomen fish sperm motility rate and vigor | |
| CN111972324B (en) | Breeding and artificial propagation method of schizothorax prenanti | |
| CN110178766B (en) | Artificial breeding method of Acipenser lapipenser | |
| CN107926770A (en) | Epinephelus akaara and epinephelus lanceolatus fish distant hybrid breeding method | |
| CN109874707B (en) | Method for efficiently creating allooctaploid silver crucian carp | |
| CN106417117A (en) | Yalong River schizothorax wangchiachii artificial proliferation and release method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |