CN106359183A - Coregonus autumnalis sperm compound activation liquid and preparation method thereof - Google Patents
Coregonus autumnalis sperm compound activation liquid and preparation method thereof Download PDFInfo
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- CN106359183A CN106359183A CN201610744590.8A CN201610744590A CN106359183A CN 106359183 A CN106359183 A CN 106359183A CN 201610744590 A CN201610744590 A CN 201610744590A CN 106359183 A CN106359183 A CN 106359183A
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- 150000001875 compounds Chemical class 0.000 title claims abstract description 60
- 239000007788 liquid Substances 0.000 title claims abstract description 56
- 230000004913 activation Effects 0.000 title claims abstract description 45
- 241001125816 Coregonus autumnalis Species 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 46
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 24
- 239000011780 sodium chloride Substances 0.000 claims abstract description 22
- 239000008103 glucose Substances 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 17
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 28
- 238000004458 analytical method Methods 0.000 claims description 6
- 239000012467 final product Substances 0.000 claims description 4
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 239000006166 lysate Substances 0.000 claims description 3
- 239000011435 rock Substances 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 2
- 238000004821 distillation Methods 0.000 claims 1
- 230000033001 locomotion Effects 0.000 abstract description 20
- 210000000582 semen Anatomy 0.000 abstract description 13
- 150000003839 salts Chemical class 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 8
- 230000008901 benefit Effects 0.000 abstract description 5
- 150000001720 carbohydrates Chemical class 0.000 abstract description 5
- 238000002474 experimental method Methods 0.000 abstract description 4
- 230000012447 hatching Effects 0.000 abstract description 4
- 230000002401 inhibitory effect Effects 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 2
- 235000014633 carbohydrates Nutrition 0.000 abstract 2
- 241000251468 Actinopterygii Species 0.000 description 16
- 235000019688 fish Nutrition 0.000 description 16
- 230000004720 fertilization Effects 0.000 description 5
- 230000003204 osmotic effect Effects 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000002045 lasting effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004899 motility Effects 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 244000153158 Ammi visnaga Species 0.000 description 1
- 235000010585 Ammi visnaga Nutrition 0.000 description 1
- 241000208340 Araliaceae Species 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241001125817 Coregonus Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 241000277331 Salmonidae Species 0.000 description 1
- 235000009392 Vitis Nutrition 0.000 description 1
- 241000219095 Vitis Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 230000013439 flagellum movement Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 210000002149 gonad Anatomy 0.000 description 1
- 230000026109 gonad development Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 230000019100 sperm motility Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention provides coregonus autumnalis sperm compound activation liquid. The sperm compound activation liquid consists of glucose and sodium chloride, wherein sodium chloride has a certain inhibiting effect on saprolegniasis in a hatching process of coregonus autumnalis fries, and glucose has a relatively good activation effect on coregonus autumnalis sperms. According to the coregonus autumnalis sperm compound activation liquid, through scientific proportioning, the usage amount of carbohydrates is reduced, meanwhile, the usage amount of salts is increased, and not only the good activation effect of carbohydrates but also the good saprolegniasis inhibiting effect of salts are utilized to realize complementation of advantages. Experiments find that if the semen is not activated by the sperm compound activation liquid, the rapid movement activation rate of sperms is 80%, and the rapid movement time of sperms lasts for 20s; and if the semen is activated by the sperm compound activation liquid, the rapid movement activation rate of sperms is over 95%, and the rapid movement time of sperms lasts for 55.2s, and thus, the sperm compound activation liquid can obviously improve the activity of coregonus autumnalis sperms.
Description
Technical field
The invention belongs to activation of spermatozoa liquid technical field is and in particular to a kind of Coregonus autumnalis sperm compound recipe activates liquid and its joins
Method processed.
Background technology
Coregonus autumnalis (coregonus autumnalis) originate in Russia, belong to salmon shape mesh, salmonidae, Coregonus.For writing
The cold aqueous economic fish of name, its meat is fine and smooth, pierces, delicious flavour no between flesh, nutritious, is deeply liked by consumers in general,
Xinjiang of China Sayram Lake was introduced Coregonus autumnalis eyed eggs from Russia first in 1998 and is carried out artificial cultivation.With China's warp
The continuous development of Ji, the continuous expansion of cold water fish consumption market, the cultivation demand of the cold aqueous high-end aquatic products such as Coregonus autumnalis is not
Disconnected expansion.
But due to the reasons such as Coregonus autumnalis migration spawning characteristic, Reproduction Conditions harshness, the Coregonus autumnalis seed yield of China
It is constantly in the state that supply falls short of demand, the cultivation hindering Coregonus autumnalis is promoted.During China's Coregonus autumnalis artificial propagation
Find, Coregonus autumnalis milter generally existing lack of quantity of spermatic fluid, the relatively low problem of rate of fertilization, directly affects artificial incubation emergence rate,
Thus leading to the low of Coregonus autumnalis Seedling production efficiency.
In Coregonus autumnalis fertilization process, when seminal fluid is when milter gonad enters water, sperm just activates derepression shape
State thus obtaining motor capacity, and swim to ovum in combination complete be fertilized；In normal water body, most of freshwater fish sperm swashs
After work, movement time is 40-60s, and movement time in Sayram Lake lake water for the Coregonus autumnalis sperm is only 24s,
Directly affects artificial incubation emergence rate, thus leading to the low of Coregonus autumnalis Seedling production efficiency.
Content of the invention
In order to solve the problems referred to above of prior art presence, the invention provides a kind of Coregonus autumnalis sperm compound recipe activation liquid
And its compound method.Described sperm compound recipe activation liquid is made up of glucose, sodium chloride, same in the usage amount reducing glucide
When, increased the usage amount of salts substances again, serve the activation that both make use of glucide good, make use of salt again
The good suppression fish molds of material are had an effect, and advantage is complementary.
It has been found that the breathing that the sperm motility energy supply of Coregonus autumnalis mostlys come from spermatid mitochondrial is made
With providing, but its most of energy is mainly used in the regulation of sperm osmotic pressure to external world, is only used for spermatic flagellum motion on a small quantity and works as
In.Therefore, how to reduce the consumption that sperm is adjusted in osmotic pressure, allow more energy can be used by the flagellar movement of sperm, from
And extend the movement time of Coregonus autumnalis sperm, make the chance that sperm is combined with ovum higher, thus improving rate of fertilization.For this
Problem, the method for solution is to replace raw water body to enter line activating to Coregonus autumnalis sperm using the solution with certain osmotic pressure, from
And reduce the regulation loss that inside and outside sperm, osmotic pressure difference is brought, extend the movement time of sperm, lift its rate of fertilization.
In Coregonus autumnalis artificial propagation, cut both ways using salt and saccharide folk prescription activation liquid, using glucose
During with the saccharide folk prescriptions such as Fructose activation liquid, the activation effect of Coregonus autumnalis sperm is good, but can lead to fish moldss during fry hatching
The generation of disease；During using the salt folk prescription such as nacl activation liquid, slightly worse to the activation effect of Coregonus autumnalis sperm, but to fry hatching
During fish molds have certain inhibitory action.Therefore, it is necessary to develop and invention Coregonus autumnalis salt and saccharide combination
Compound recipe activation liquid, play the complementary effect of salt and glucide advantage.
The technical solution adopted in the present invention is: a kind of Coregonus autumnalis sperm compound recipe activates liquid, including component and component
Concentration is: glucose 85-163.5mmol/l, sodium chloride 40-55mmol/l, and the temperature that described sperm compound recipe activates liquid is 5-8
℃.
Preferably, the component that described sperm compound recipe activation liquid includes and concentration of component are: glucose 85-163.5mmol/l,
Sodium chloride 40-48mmol/l.
Preferably, the component that described sperm compound recipe activation liquid includes and concentration of component are: glucose 85-146.8mmol/l,
Sodium chloride 40-48mmol/l.
Further, the component that described sperm compound recipe activation liquid includes and concentration of component are: glucose 114mmol/l, chlorine
Change sodium 55mmol/l.
Present invention also offers a kind of described sperm compound recipe activates the compound method of liquid, comprise the following steps:
A, according to described concentration of component, weigh glucose and sodium chloride；
B, the glucose weighing and sodium chloride are placed in container, add water to concentration of component, that is, obtain room temperature sperm multiple
Side's activation liquid；
C, before use, solution is cooled to 5-8 DEG C, obtains final product described sperm compound recipe activation liquid.
In step a, weigh the pure glucose of analysis and analysis pure sodium chloride using electronic balance.
In step b, using a part of water, described glucose and sodium chloride are dissolved in beaker, and lysate is transferred to
In volumetric flask, reuse water washing beaker two to three times, and cleaning mixture is transferred in volumetric flask, rock uniformly after constant volume, that is,
Obtain room temperature sperm compound recipe activation liquid；Described water is deionized water or distilled water.
The invention has the benefit that
1 the invention provides a kind of Coregonus autumnalis sperm compound recipe activation liquid, described sperm compound recipe activation liquid by glucose,
Sodium chloride forms, and wherein sodium chloride has certain inhibitory action, Fructus Vitis viniferae to fish molds during Coregonus autumnalis fry hatching
Sugar is preferable to the activation effect of Coregonus autumnalis sperm, and the present invention passes through scientific matching, in the usage amount reducing glucide simultaneously,
Increased the usage amount of salts substances again, serve the activation that both make use of glucide good, make use of salt thing again
The good suppression fish molds of matter are had an effect, and advantage is complementary.Experiment finds, does not use sperm compound recipe to activate the seminal fluid of liquid activation,
Quickly motion-activated ratio is 80% to its sperm, the lasting 20s of the quick movement time of sperm about；Swashed using sperm compound recipe activation liquid
The seminal fluid lived, quickly motion-activated ratio is more than 95% to its sperm, the lasting 55.2s of the quick movement time of sperm about, described
Sperm compound recipe activation liquid energy enough significantly improves Coregonus autumnalis motility of sperm.
2 present invention also offers a kind of described sperm compound recipe activates the compound method of liquid, described compound method operation letter
List is it is easy to promote.
Specific embodiment
With reference to embodiment, further illustrate present disclosure.It should be appreciated that the present invention implement do not limit to
In the following examples, any pro forma flexible and/or change that the present invention is made falls within the scope of the present invention.
In the present invention, if not refering in particular to, all of part, percentage ratio are unit of weight, and all of equipment and raw material etc. are all
Be commercially available or the industry commonly use.Method in following embodiments, if no special instructions, is the routine of this area
Method.
Embodiment 1
The present embodiment is the embodiment that Coregonus autumnalis sperm compound recipe activates liquid different ratio consumption and compound method.
A kind of Coregonus autumnalis sperm compound recipe activates liquid, including component and concentration of component as shown in table 1:
Table 1: sperm compound recipe activation liquid different ratio scale
Above-mentioned sperm compound recipe activates the compound method of liquid, comprises the following steps:
A, activate the volume of liquid according to described concentration of component and final sperm compound recipe, calculate the use of glucose and sodium chloride
Amount, and weigh the pure glucose of analysis and analysis pure sodium chloride using electronic balance；
B, using a part of water, described glucose and sodium chloride are dissolved in beaker, and lysate is transferred to volumetric flask
In, reuse water washing beaker two to three times, and cleaning mixture is transferred in volumetric flask, rock after constant volume uniformly, that is, often obtain
Warm sperm compound recipe activates liquid；Described water is deionized water or distilled water；
C, before use, solution is cooled to 5-8 DEG C, obtains final product described sperm compound recipe activation liquid.
Embodiment 2
The present embodiment is the activation of spermatozoa experiment that in embodiment 1, sperm compound recipe activates liquid.
First, parent fish selects
Milter 10 is selected in test altogether, and average weight is 532g.Selected parent fish physique healthy and strong, no hinders, gonad development in vitro
Maturation, light pressure abdominal part seminal fluid can flow out automatically.
2nd, semen collection
Respectively catch parent fish using parent fish folder or two people end to end, after the parent fish of Baoding, a people first gently erases parent fish life with towel
Grow the body surface moisture around hole, then slightly oppress along fish belly both sides, so that seminal fluid is flowed in measuring cup.
3rd, motility of sperm is observed and detection method
During observation, prior to a reagent (lake water or described sperm compound recipe activation liquid) is added on microscope slide, with clean toothpick point
End a little seminal fluid of picking mixed, at once move under 10 × 40 power microscopes, be sequentially completed same milt liquid lake water with
Described sperm compound recipe activates the quick movement observations in liquid.Observe and be defined by a visual field.When seminal fluid is mixed with reagent phase, immediately
Start timing with stopwatch.When the sperm having more than 70% switchs to middling speed motion from quick motion, pinch table immediately, obtain final product quick fortune
The dynamic time.In the present embodiment, the sperm compound recipe from serial number in embodiment 11,3 and 5 activates liquid, is contrasted with lake water.
4th, experimental result
The present embodiment, is activated at liquid using 3 kinds of sperm compound recipes in river water and embodiment 1 respectively to the seminal fluid of every parent fish
Reason, result is as shown in table 2:
Table 2 experimental result contrast table
| Experiment group | Test number (TN)/time | Quick movement time/s |
| Lake water group | 10 | 20.7±2.9 |
| 1 group of sequence number | 10 | 51.1±4.9 |
| 3 groups of sequence number | 10 | 55.2±6.7 |
| 4 groups of sequence number | 10 | 59.3±5.4 |
Using the seminal fluid of lake water activation, quickly motion-activated ratio is 80% to its sperm；Using the activation of described sperm compound recipe
The seminal fluid of liquid activation, quickly motion-activated ratio is more than 95% to its sperm.
Test result indicate that, higher essence can be obtained using the Coregonus autumnalis sperm that described sperm compound recipe activates after liquid activation
The quick movement time of son, can effectively improve the vigor of Coregonus autumnalis sperm, and this is most important to improving rate of fertilization.
It should be noted last that, above example only in order to technical scheme to be described and unrestricted, although ginseng
According to preferred embodiment, the present invention is described in detail, should be understood that and the foregoing is only being embodied as of the present invention
Mode, the protection domain being not intended to limit the present invention, all within the spirit and principles in the present invention, done any repair
Change, equivalent, improvement etc., should be included within the scope of the present invention.
Claims (8)
1. a kind of Coregonus autumnalis sperm compound recipe activates liquid it is characterised in that the component including and concentration of component are: glucose 85-
163.5mmol/l, sodium chloride 40-55mmol/l, the temperature that described sperm compound recipe activates liquid is 5-8 DEG C.
2. sperm compound recipe according to claim 1 activates liquid it is characterised in that the component including and concentration of component are: Portugal
Grape sugar 85-163.5mmol/l, sodium chloride 40-48mmol/l.
3. sperm compound recipe according to claim 2 activates liquid it is characterised in that the component including and concentration of component are: Portugal
Grape sugar 85-146.8mmol/l, sodium chloride 40-48mmol/l.
4. sperm compound recipe according to claim 3 activates liquid it is characterised in that the component including and concentration of component are: Portugal
Grape sugar 114mmol/l, sodium chloride 55mmol/l.
5. a kind of arbitrary described sperm compound recipe of claim 1-4 activates the compound method of liquid it is characterised in that including following walking
Rapid:
A, according to described concentration of component, weigh glucose and sodium chloride；
B, the glucose weighing and sodium chloride are placed in container, add water to concentration of component, that is, obtain room temperature sperm compound recipe and swash
Liquid alive；
C, before use, solution is cooled to 5-8 DEG C, obtains final product described sperm compound recipe activation liquid.
6. compound method according to claim 5 is it is characterised in that in step a, weigh the pure Portugal of analysis using electronic balance
Grape sugar and analysis pure sodium chloride.
7. compound method according to claim 5 is it is characterised in that in step b, using a part of water by described glucose
Dissolve in beaker with sodium chloride, and lysate is transferred in volumetric flask, reuse water washing beaker two to three times, and will wash
Wash liquid to be transferred in volumetric flask, rock after constant volume uniformly, that is, obtain room temperature sperm compound recipe activation liquid.
8. compound method according to claim 5 is it is characterised in that in step b, described water is deionized water or distillation
Water.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108244001A (en) * | 2018-02-08 | 2018-07-06 | 西藏自治区农牧科学院水产科学研究所 | A kind of method for improving different fissure abdomen fish sperm motility rate and vigor |
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2016
- 2016-08-26 CN CN201610744590.8A patent/CN106359183A/en active Pending
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| RU2035858C1 (en) * | 1992-07-02 | 1995-05-27 | Акционерное общество закрытого типа "Акватрон" | Method for treatment of fish sperm |
| CN102578076A (en) * | 2012-01-11 | 2012-07-18 | 中国水产科学研究院长江水产研究所 | Universal diluent for tilapia sperm and preparation method thereof |
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| Title |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108244001A (en) * | 2018-02-08 | 2018-07-06 | 西藏自治区农牧科学院水产科学研究所 | A kind of method for improving different fissure abdomen fish sperm motility rate and vigor |
| CN108244001B (en) * | 2018-02-08 | 2020-12-11 | 西藏自治区农牧科学院水产科学研究所 | Method for improving sperm motility rate and vitality of schizothorax prenanti |
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Application publication date: 20170201 |