CN108048349A - 副干酪乳杆菌n1115包埋菌粉的制备方法及其应用 - Google Patents
副干酪乳杆菌n1115包埋菌粉的制备方法及其应用 Download PDFInfo
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- CN108048349A CN108048349A CN201711352459.8A CN201711352459A CN108048349A CN 108048349 A CN108048349 A CN 108048349A CN 201711352459 A CN201711352459 A CN 201711352459A CN 108048349 A CN108048349 A CN 108048349A
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- lactobacillus paracasei
- bacterium
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- bacterium powder
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- Granted
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- 241000894006 Bacteria Species 0.000 title claims abstract description 66
- 239000000843 powder Substances 0.000 title claims abstract description 37
- 241000186315 Lactobacillus paracasei N1115 Species 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- 239000003223 protective agent Substances 0.000 claims abstract description 19
- 239000006041 probiotic Substances 0.000 claims abstract description 14
- 235000018291 probiotics Nutrition 0.000 claims abstract description 14
- 235000021001 fermented dairy product Nutrition 0.000 claims abstract description 6
- 238000012545 processing Methods 0.000 claims abstract description 4
- 230000001954 sterilising effect Effects 0.000 claims description 9
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 8
- 238000005119 centrifugation Methods 0.000 claims description 6
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 4
- 235000011187 glycerol Nutrition 0.000 claims description 4
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 3
- 229920001661 Chitosan Polymers 0.000 claims description 3
- 108010010803 Gelatin Proteins 0.000 claims description 3
- 239000005862 Whey Substances 0.000 claims description 3
- 102000007544 Whey Proteins Human genes 0.000 claims description 3
- 108010046377 Whey Proteins Proteins 0.000 claims description 3
- 239000008273 gelatin Substances 0.000 claims description 3
- 229920000159 gelatin Polymers 0.000 claims description 3
- 235000019322 gelatine Nutrition 0.000 claims description 3
- 235000011852 gelatine desserts Nutrition 0.000 claims description 3
- 230000036571 hydration Effects 0.000 claims description 3
- 238000006703 hydration reaction Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims description 3
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims description 3
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims description 3
- 235000010413 sodium alginate Nutrition 0.000 claims description 3
- 239000000661 sodium alginate Substances 0.000 claims description 3
- 229940005550 sodium alginate Drugs 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 238000010792 warming Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 230000001580 bacterial effect Effects 0.000 abstract description 27
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 abstract description 14
- 238000000034 method Methods 0.000 abstract description 12
- 230000004083 survival effect Effects 0.000 abstract description 12
- 241000186605 Lactobacillus paracasei Species 0.000 abstract description 9
- 210000000941 bile Anatomy 0.000 abstract description 7
- 210000004051 gastric juice Anatomy 0.000 abstract description 7
- 230000001681 protective effect Effects 0.000 abstract description 5
- 239000000243 solution Substances 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 235000013618 yogurt Nutrition 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
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- 241000186660 Lactobacillus Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
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- 239000012895 dilution Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 229940039696 lactobacillus Drugs 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 235000013406 prebiotics Nutrition 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
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- 239000000725 suspension Substances 0.000 description 2
- 206010011732 Cyst Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 201000010538 Lactose Intolerance Diseases 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000020167 acidified milk Nutrition 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000013532 brandy Nutrition 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 208000031513 cyst Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000002183 duodenal effect Effects 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 235000013332 fish product Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 210000000232 gallbladder Anatomy 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 235000015141 kefir Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000003094 microcapsule Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000021110 pickles Nutrition 0.000 description 1
- 229920006254 polymer film Polymers 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 235000021262 sour milk Nutrition 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
Description
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CN201711352459.8A CN108048349B (zh) | 2017-12-15 | 2017-12-15 | 副干酪乳杆菌n1115包埋菌粉的制备方法及其应用 |
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CN201711352459.8A CN108048349B (zh) | 2017-12-15 | 2017-12-15 | 副干酪乳杆菌n1115包埋菌粉的制备方法及其应用 |
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CN108048349B CN108048349B (zh) | 2020-08-28 |
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CN201711352459.8A Active CN108048349B (zh) | 2017-12-15 | 2017-12-15 | 副干酪乳杆菌n1115包埋菌粉的制备方法及其应用 |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111286469A (zh) * | 2018-12-10 | 2020-06-16 | 东北农业大学 | 一种副干酪乳杆菌冻干菌粉的制备方法 |
CN111317140A (zh) * | 2020-05-06 | 2020-06-23 | 永安康健药业(武汉)有限公司 | 一种耐胆盐耐胃酸益生菌制剂及其制备方法和应用 |
CN111480849A (zh) * | 2019-12-11 | 2020-08-04 | 石家庄君乐宝乳业有限公司 | 母乳化的复合益生菌微胶囊粉及其制备方法和应用 |
CN112458029A (zh) * | 2020-12-21 | 2021-03-09 | 苏州微克生活科技有限公司 | 一种高活性副干酪乳杆菌冻干粉的制备方法及应用 |
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CN1843385A (zh) * | 2006-02-17 | 2006-10-11 | 哈尔滨美华生物技术股份有限公司 | 肠道微生态制剂及其制备方法 |
CN1916161A (zh) * | 2006-09-08 | 2007-02-21 | 肖雯娟 | 一种肠道益生菌包埋保护方法 |
CN104644612A (zh) * | 2015-01-26 | 2015-05-27 | 天津科技大学 | 聚丙烯酸钠接枝海藻酸钠包埋益生菌微胶囊及其制备方法 |
CN105919970A (zh) * | 2016-06-02 | 2016-09-07 | 天津欣益源科技发展有限公司 | 一种用于提高益生菌生物活性的包埋微胶囊 |
CN106689394A (zh) * | 2017-01-08 | 2017-05-24 | 东北农业大学 | 含有微胶囊化降胆固醇植物乳杆菌的white‑brined干酪及其制备方法 |
CN107007633A (zh) * | 2016-01-27 | 2017-08-04 | 聊城市畜牧兽医局 | 一种益生菌多糖复合制剂制备方法 |
CN107156575A (zh) * | 2017-05-26 | 2017-09-15 | 江苏碧青园海洋生物科技有限公司 | 一种益生菌固体饮料的制作方法 |
-
2017
- 2017-12-15 CN CN201711352459.8A patent/CN108048349B/zh active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1843385A (zh) * | 2006-02-17 | 2006-10-11 | 哈尔滨美华生物技术股份有限公司 | 肠道微生态制剂及其制备方法 |
CN1916161A (zh) * | 2006-09-08 | 2007-02-21 | 肖雯娟 | 一种肠道益生菌包埋保护方法 |
CN104644612A (zh) * | 2015-01-26 | 2015-05-27 | 天津科技大学 | 聚丙烯酸钠接枝海藻酸钠包埋益生菌微胶囊及其制备方法 |
CN107007633A (zh) * | 2016-01-27 | 2017-08-04 | 聊城市畜牧兽医局 | 一种益生菌多糖复合制剂制备方法 |
CN105919970A (zh) * | 2016-06-02 | 2016-09-07 | 天津欣益源科技发展有限公司 | 一种用于提高益生菌生物活性的包埋微胶囊 |
CN106689394A (zh) * | 2017-01-08 | 2017-05-24 | 东北农业大学 | 含有微胶囊化降胆固醇植物乳杆菌的white‑brined干酪及其制备方法 |
CN107156575A (zh) * | 2017-05-26 | 2017-09-15 | 江苏碧青园海洋生物科技有限公司 | 一种益生菌固体饮料的制作方法 |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111286469A (zh) * | 2018-12-10 | 2020-06-16 | 东北农业大学 | 一种副干酪乳杆菌冻干菌粉的制备方法 |
CN111286469B (zh) * | 2018-12-10 | 2022-11-01 | 东北农业大学 | 一种副干酪乳杆菌冻干菌粉的制备方法 |
CN111480849A (zh) * | 2019-12-11 | 2020-08-04 | 石家庄君乐宝乳业有限公司 | 母乳化的复合益生菌微胶囊粉及其制备方法和应用 |
CN111317140A (zh) * | 2020-05-06 | 2020-06-23 | 永安康健药业(武汉)有限公司 | 一种耐胆盐耐胃酸益生菌制剂及其制备方法和应用 |
CN112458029A (zh) * | 2020-12-21 | 2021-03-09 | 苏州微克生活科技有限公司 | 一种高活性副干酪乳杆菌冻干粉的制备方法及应用 |
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