CN108040845A - A kind of method for culturing seedlings of oil Paeonia suffruticosa seed - Google Patents
A kind of method for culturing seedlings of oil Paeonia suffruticosa seed Download PDFInfo
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- CN108040845A CN108040845A CN201711207022.5A CN201711207022A CN108040845A CN 108040845 A CN108040845 A CN 108040845A CN 201711207022 A CN201711207022 A CN 201711207022A CN 108040845 A CN108040845 A CN 108040845A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
Abstract
The invention discloses a kind of method for culturing seedlings of oil Paeonia suffruticosa seed, comprise the following steps：(1) seed collection urges root culture, (3) presprouting of seeds nursery, (4) transplanting and final-period management with processing, (2) seed.A kind of method for culturing seedlings of oil Paeonia suffruticosa seed of the present invention can effectively break the dormancy of Paeonia suffruticosa seed, and the rate that roots is high, and speed of germinating is fast, simple to operate, is suitble to large-scale promotion.
Description
Technical field
The present invention relates to the method for culturing seedlings of Paeonia suffruticosa seed, more particularly to a kind of method for culturing seedlings of oil Paeonia suffruticosa seed.
Background technology
Tree peony (Classification system：Paeonia suffruticosaAndr.), it is Ranunculaceae Paeonia, belongs to machaka and plant
Object.Paeonia suffruticosa seed endosperm is loose, and rich in grease, carbohydrate, amino acid, protein and vitamin etc., 17 kinds are shared in seed oil
Fatty acid composition is a kind of good oil resource.
Paeonia suffruticosa seed has the characteristic of typical epicotyl and the dual dormancy of hypocotyl, in its natural state, Paeonia suffruticosa seed
Germination process show stage, mature seed under the conditions of warm temperature hypocotyl elongation, take root, take root seed after low temperature on
Plumular axis termination of diapause, then germinate and be unearthed under the conditions of warm temperature, then growth of seedling.However, in the reproductive process of Paeonia suffruticosa seed,
Seed is often not thorough because dormancy is not released or released, and reduces germination percentage, influences cultivation production.
Phoenix pellet tree peony is medicine oil dual-purpose breed, and peony seed oil unsaturated fatty acid content is 92%, polyunsaturated fatty acid
Content is 70%, and wherein linolenic acid content is more than 40%, is 140 times of olive oil, 10 times of soybean oil, be a kind of good
Oil resource.Phoenix pellet Paeonia suffruticosa seed sprouts difficulty, and growing-seedling period is long, and seedling quality is not high, seriously constrains the development of industry, because
This, sprouts according to Paeonia suffruticosa seed and Seedling Growth Characteristics, the correlative study for carrying out phoenix pellet tree peony seedling-raising technique are significant.
Nano material refers to that structural unit size is at least one-dimensional in nanoscale scope or by it in three dimensions
As elementary cell form material.Nano material the sprouting of seed and the growth effect of plant are mainly shown as promotion,
Inhibit and the three classes that do not make significant difference.Meanwhile influence of the nano material of the same race to different plants is different, i.e. the plant life of nano material
Object effect has species difference.At present on nano materials such as nano-titanium oxide, nano silver, carbon nanotubes to cucumber, western red
Persimmon, soybean growth and development research it is very much, for Paeonia suffruticosa seed sprout, growth research it is seldom.
Nano-titanium oxide, nano-sized iron oxide and nano zine oxide are applied to the taking root of oil Paeonia suffruticosa seed, urged by the present invention
In bud, the rooting rate and germination percentage of oil Paeonia suffruticosa seed are improved.
The content of the invention
The present invention's provides a kind of method for culturing seedlings of oil Paeonia suffruticosa seed.The present invention adopts the following technical scheme that：
A kind of method for culturing seedlings of oil Paeonia suffruticosa seed, comprises the following steps：
(1) seed collection and processing：The ripening fruits of mid or late August phoenix pellet tree peony is gathered, is placed on airing at shady and cool ventilation,
When fruit natural cracking, seed is taken out, and ripe seed is selected with wet cleaning process；
(2) seed urges root culture：It will be rinsed well after seed disinfection with clear water, drain the surface of the seed moisture, seed is existed
25-35 DEG C, quality for 4-6 times of seed quality water in impregnate 9-15 it is small when, taking-up seed, when airing 6-12 is small in the cool,
Then seed is placed in the hole tray equipped with matrix I, a seed is put in each hole tray, hole tray is placed on 20-30 DEG C artificial
Culture grows the root of more than 3cm to seed in climate box, and humidity is 75-90% in growth cabinet, daily illumination during culture
12h, flux density 3000-12000lx, dark 12h；
The matrix I is that river sand, plant ash, perlite are 2 in mass ratio:1:1 mixture；
(3) presprouting of seeds nursery：The seed for the root for growing more than 3cm after step (2) culture is placed in equipped with matrix II
Hole tray in, a seed is put in each hole tray, under natural lighting culture grow two panels true leaf to seed；The matrix II is river
Husky, vermiculite is 4 in mass ratio:1 mixture；
(4) transplanting and final-period management：The seedling for growing two panels true leaf is transplanted in sand bed under natural light and is cultivated, uses sunshade
Net shading, it is 40-60% that daily water drenching, which keeps sand bed humidity,；Husky thickness 15-17cm in the sand bed, after seedling is transplanted to sand bed
Seedling spacing is 35-55cm, and sand used in sand bed is river sand.
A kind of method for culturing seedlings of oil Paeonia suffruticosa seed, comprises the following steps：
(1) seed collection and processing：The ripening fruits of mid or late August phoenix pellet tree peony is gathered, is placed on airing at shady and cool ventilation,
When fruit natural cracking, seed is taken out, and ripe seed is selected with wet cleaning process；
(2) seed urges root culture：It will be rinsed well after seed disinfection with clear water, drain the surface of the seed moisture, seed is existed
25-35 DEG C, quality for 4-6 times of seed quality seed-soaking liquid in impregnate 9-15 it is small when, taking-up seed, airing 6-12 is small in the cool
When, then seed is placed in the hole tray equipped with matrix I, a seed is put in each hole tray, hole tray is placed on to 20-30 DEG C of people
Culture grows the root of more than 3cm to seed in work climate box, and humidity is 75-90% in growth cabinet, daily light during culture
According to 12h, flux density 3000-12000lx, dark 12h；
The matrix I is that river sand, plant ash, perlite are 2 in mass ratio:1:1 mixture；The preparation of the seed-soaking liquid
Method is as follows：By 10-40mg potassium sulfates, 8-32mg sulfuric acid, 100-200mg vitamin B1s, 30-70mg thiocarbamides and 30-70mg mountains
Pears acid is added in the 500-1000g water that temperature is 25-50 DEG C, is stirred 30-60 minutes, obtained with 500-1000 revs/min of rotating speed
To seed-soaking liquid；
(3) presprouting of seeds nursery：The seed for the root for growing more than 3cm after step (2) culture is placed in equipped with matrix II
Hole tray in, a seed is put in each hole tray, under natural lighting culture grow two panels true leaf to seed；The matrix II is river
Husky, vermiculite is 4 in mass ratio:1 mixture；
(4) transplanting and final-period management：The seedling for growing two panels true leaf is transplanted in sand bed under natural light and is cultivated, uses sunshade
Net shading, it is 40-60% that daily water drenching, which keeps sand bed humidity,；Husky thickness 15-17cm in the sand bed, after seedling is transplanted to sand bed
Seedling spacing is 35-55cm, and sand used in sand bed is river sand.
A kind of method for culturing seedlings of oil Paeonia suffruticosa seed, comprises the following steps：
(1) seed collection and processing：The ripening fruits of mid or late August phoenix pellet tree peony is gathered, is placed on airing at shady and cool ventilation,
When fruit natural cracking, seed is taken out, and ripe seed is selected with wet cleaning process；
(2) seed urges root culture：It will be rinsed well after seed disinfection with clear water, drain the surface of the seed moisture, seed is existed
25-35 DEG C, quality for 4-6 times of seed quality seed-soaking liquid in impregnate 9-15 it is small when, taking-up seed, airing 6-12 is small in the cool
When, then seed is placed in the hole tray equipped with matrix I, a seed is put in each hole tray, hole tray is placed on to 20-30 DEG C of people
Culture grows the root of more than 3cm to seed in work climate box, and humidity is 75-90% in growth cabinet, daily light during culture
According to 12h, flux density 3000-12000lx, with the ultra violet lamp that wavelength is 254-395nm, power is 10-40W after illumination
Hole tray 15-45min, ultraviolet lamp is located at directly over hole tray, horizontal positioned, and vertical range of the ultraviolet lamp apart from hole tray is 40-80 lis
Rice, remaining time is interlunation；
The matrix I is that river sand, plant ash, perlite are 2 in mass ratio:1:1 mixture；The preparation of the seed-soaking liquid
Method is as follows：By 10-40mg potassium sulfates, 8-32mg sulfuric acid, 100-200mg vitamin B1s, 30-70mg thiocarbamides, 0.15-1.5mg
Nano-titanium oxide and 30-70mg sorbic acids are added in the 500-1000g water that temperature is 25-50 DEG C, with 500-1000 revs/min
Rotating speed stirs 30-60 minutes, obtains seed-soaking liquid；
(3) presprouting of seeds nursery：The seed for the root for growing more than 3cm after step (2) culture is placed in equipped with matrix II
Hole tray in, a seed is put in each hole tray, under natural lighting culture grow two panels true leaf to seed；The matrix II is river
Husky, vermiculite is 4 in mass ratio:1 mixture；
(4) transplanting and final-period management：The seedling for growing two panels true leaf is transplanted in sand bed under natural light and is cultivated, uses sunshade
Net shading, it is 40-60% that daily water drenching, which keeps sand bed humidity,；Husky thickness 15-17cm in the sand bed, after seedling is transplanted to sand bed
Seedling spacing is 35-55cm, and sand used in sand bed is river sand.
A kind of method for culturing seedlings of oil Paeonia suffruticosa seed, comprises the following steps：
(1) seed collection and processing：The ripening fruits of mid or late August phoenix pellet tree peony is gathered, is placed on airing at shady and cool ventilation,
When fruit natural cracking, seed is taken out, and ripe seed is selected with wet cleaning process；
(2) seed urges root culture：It will be rinsed well after seed disinfection with clear water, drain the surface of the seed moisture, seed is existed
25-35 DEG C, quality for 4-6 times of seed quality seed-soaking liquid in impregnate 9-15 it is small when, taking-up seed, airing 6-12 is small in the cool
When, then seed is placed in the hole tray equipped with matrix I, a seed is put in each hole tray, hole tray is placed on to 20-30 DEG C of people
Culture grows the root of more than 3cm to seed in work climate box, and humidity is 75-90% in growth cabinet, daily light during culture
According to 12h, flux density 3000-12000lx, with the ultra violet lamp that wavelength is 254-395nm, power is 10-40W after illumination
Hole tray 15-45min, ultraviolet lamp is located at directly over hole tray, horizontal positioned, and vertical range of the ultraviolet lamp apart from hole tray is 40-80 lis
Rice, remaining time is interlunation；
The matrix I is that river sand, plant ash, perlite are 2 in mass ratio:1:1 mixture；The preparation of the seed-soaking liquid
Method is as follows：By 10-40mg potassium sulfates, 8-32mg sulfuric acid, 100-200mg vitamin B1s, 30-70mg thiocarbamides, 0.15-1.5mg
Nano-titanium oxide and 30-70mg sorbic acids are added in the 500-1000g water that temperature is 25-50 DEG C, with 500-1000 revs/min
Rotating speed stirs 30-60 minutes, obtains seed-soaking liquid；
(3) presprouting of seeds nursery：The seed for the root for growing more than 3cm after step (2) culture is placed in equipped with matrix II
Hole tray in, a seed is put in each hole tray, daily 50-200 milliliters of the nutrient solution of middle sprinkling into each hole tray, in nature
Culture to seed grows two panels true leaf under illumination；
The preparation method of the nutrient solution is as follows, and described part is parts by weight：By dried peppermint leaf, pomegranate leaf, perilla leaf difference
200 mesh sieves are crushed, take 25-50 parts dried peppermint leaf, 25-35 parts of pomegranate leafs, 15-45 parts of perilla leafs and 5-10 parts of white sands after crushing
Sugar, 1-5 parts of lactobacillus plantarums, 60-100 parts of water are added in fermentation tank after mixing, and 20-35 DEG C is sealed by fermentation 20-40 days,
Mixture after fermentation is crossed into 300 mesh sieves, retains filtrate to get nutrient solution；
The matrix II is that river sand, vermiculite are 4 in mass ratio:1 mixture；
(4) transplanting and final-period management：The seedling for growing two panels true leaf is transplanted in sand bed under natural light and is cultivated, uses sunshade
Net shading, it is 40-60% that daily water drenching, which keeps sand bed humidity,；Husky thickness 15-17cm in the sand bed, after seedling is transplanted to sand bed
Seedling spacing is 35-55cm, and sand used in sand bed is river sand.
A kind of method for culturing seedlings of oil Paeonia suffruticosa seed, comprises the following steps：
(1) seed collection and processing：The ripening fruits of mid or late August phoenix pellet tree peony is gathered, is placed on airing at shady and cool ventilation,
When fruit natural cracking, seed is taken out, and ripe seed is selected with wet cleaning process；
(2) seed urges root culture：It will be rinsed well after seed disinfection with clear water, drain the surface of the seed moisture, seed is existed
25-35 DEG C, quality for 4-6 times of seed quality seed-soaking liquid in impregnate 9-15 it is small when, taking-up seed, airing 6-12 is small in the cool
When, then seed is placed in the hole tray equipped with matrix I, a seed is put in each hole tray, hole tray is placed on to 20-30 DEG C of people
Culture grows the root of more than 3cm to seed in work climate box, and humidity is 75-90% in growth cabinet, daily light during culture
According to 12h, flux density 3000-12000lx, with the ultra violet lamp that wavelength is 254-395nm, power is 10-40W after illumination
Hole tray 15-45min, ultraviolet lamp is located at directly over hole tray, horizontal positioned, and vertical range of the ultraviolet lamp apart from hole tray is 40-80 lis
Rice, remaining time is interlunation；
The matrix I is that river sand, plant ash, perlite are 2 in mass ratio:1:1 mixture；The preparation of the seed-soaking liquid
Method is as follows：By 10-40mg potassium sulfates, 8-32mg sulfuric acid, 100-200mg vitamin B1s, 30-70mg thiocarbamides, 0.15-1.5mg
Nano-titanium oxide (being preferably 0.3mg nano-titanium oxides) and 30-70mg sorbic acids are added to the 500- that temperature is 25-50 DEG C
In 1000g water, stirred 30-60 minutes with 500-1000 revs/min of rotating speed, obtain seed-soaking liquid；
(3) presprouting of seeds nursery：The seed for the root for growing more than 3cm after step (2) culture is placed in equipped with matrix II
Hole tray in, a seed is put in each hole tray, daily 50-200 milliliters of the nutrient solution of middle sprinkling into each hole tray, in nature
Culture to seed grows two panels true leaf under illumination；
The preparation method of the nutrient solution is as follows, and described part is parts by weight：By dried peppermint leaf, pomegranate leaf, perilla leaf difference
200 mesh sieves are crushed, take 25-50 parts dried peppermint leaf, 25-35 parts of pomegranate leafs, 15-45 parts of perilla leafs and 5-10 parts of white sands after crushing
Sugar, 1-5 parts of lactobacillus plantarums, 60-100 parts of water are added in fermentation tank after mixing, and 20-35 DEG C is sealed by fermentation 20-40 days,
Mixture after fermentation is crossed into 300 mesh sieves, retains filtrate to get nutrient solution；
The matrix II is that river sand, vermiculite, nano material are 4 in mass ratio:1：0.02 mixture；
The nano material is nano ferrous iron oxide and/or nano zine oxide；
(4) transplanting and final-period management：The seedling for growing two panels true leaf is transplanted in sand bed under natural light and is cultivated, uses sunshade
Net shading, it is 40-60% that daily water drenching, which keeps sand bed humidity,；Husky thickness 15-17cm in the sand bed, after seedling is transplanted to sand bed
Seedling spacing is 35-55cm, and sand used in sand bed is river sand.
Preferably, the disinfection be by seed 25 DEG C, quality be 3-6 times of seed quality, mass fraction be 5% it is secondary
It is impregnated 5 minutes in sodium chlorate solution.
Preferably, the volume that matrix I is packed into the hole tray is the 4/5 of hole tray volume.
Preferably, the volume that matrix II is packed into the hole tray is the 4/5 of hole tray volume.
Contain nano-titanium oxide in the seed-soaking liquid of the present invention, the seed for impregnating the seed-soaking liquid containing nano-titanium oxide is purple
Outside line illumination, the cell and tissue of nano-titanium oxide energy activated seed activate seed enzymatic activity, in addition ultraviolet light irradiation can excite
Seed is taken root vigor.Preferably, it is 254nm that step (2) seed, which is urged in root culture after daily illumination with wavelength, power is
The ultra violet lamp hole tray 25min of 21W, ultraviolet lamp is located at directly over hole tray, horizontal positioned, ultraviolet lamp apart from hole tray it is vertical away from
From for 40 centimetres, remaining time is interlunation.The preparation method of seed-soaking liquid is as follows used in step (2)：By 10mg potassium sulfates,
It is 25 DEG C that 8mg sulfuric acid, 100mg vitamin B1s, 30mg thiocarbamides, 0.3mg nano-titanium oxides and 30mg sorbic acids, which are added to temperature,
In 500g water, stirred 30 minutes with 500 revs/min of rotating speed, obtain seed-soaking liquid.
Preferably, the preparation method of the nutrient solution is as follows, and described part is parts by weight：By dried peppermint leaf, pomegranate leaf, purple perilla
Leaf crushed 200 mesh sieves respectively, take crush after 40 parts of dried peppermint leaves, 35 parts of pomegranate leafs, 20 parts of perilla leafs and 5 portions of white granulated sugars, 1 part
Lactobacillus plantarum, 60 parts of water are added in fermentation tank after mixing, and 30 DEG C are sealed by fermentation 25 days, by the mixture mistake after fermentation
300 mesh sieves retain filtrate to get nutrient solution.
Preferably, the nano material is that nano ferrous iron oxide and nano zine oxide are (1-3) in mass ratio:(1-3's)
Mixture, it is highly preferred that it is 3 in mass ratio that the nano material, which is nano ferrous iron oxide and nano zine oxide,:1 mixture.
A kind of method for culturing seedlings of oil Paeonia suffruticosa seed of the present invention, can effectively break the dormancy of Paeonia suffruticosa seed, root
Rate is high, and speed of germinating is fast, simple to operate, is suitble to large-scale promotion.
On the basis of common knowledge of the art, above-mentioned each optimum condition, can be each preferably to get the present invention in any combination
Embodiment.
Specific embodiment
It is further illustrated the present invention below by the mode of embodiment, but does not therefore limit the present invention to the reality
It applies among a scope.The experimental method of actual conditions is not specified in the following example, according to conventional methods and conditions or according to business
Product specification selects.
In following embodiments, primary raw material and instrument used are as follows：
Phoenix pellet tree peony is medicine oil dual-purpose breed, and peony seed oil unsaturated fatty acid content 92%, polyunsaturated fatty acid contains
Amount 70%, wherein leukotrienes are more than 40%, are 140 times of olive oil, 10 times of soybean oil, be a kind of good oil plant money
Source.Phoenix pellet peony seed oil has effects that reducing blood lipid, reduces cholesterol.
Sodium hypochlorite, No. CAS：7681-52-9, purchased from Chongqing Tianyuan Chemical Co., Ltd..
River sand, purchased from Lingshou County Shi Nuo mineral products processings factory, 70 mesh of grain size.
Plant ash, purchased from Shanghai little Fan afforestation Co., Ltd.
Perlite, purchased from Lingshou County Shi Nuo mineral products processings factory, 40 mesh of grain size.
Potassium sulfate, No. CAS：7778-80-5, purchased from Chongqing Tianyuan Chemical Co., Ltd..
Sulfuric acid, No. CAS：7664-93-9, purchased from Chongqing Tianyuan Chemical Co., Ltd., mass fraction is 98% concentrated sulfuric acid.
Vitamin B1, No. CAS：59-43-8, purchased from Chongqing Wei Dong biochemical industries Co., Ltd.
Thiocarbamide, No. CAS：62-56-6, purchased from Jining magnificence Chemical Co., Ltd..
Sorbic acid, No. CAS：110-44-1, purchased from Henan Tian Fu Chemical Co., Ltd.s.
Nano-titanium oxide, i.e., nano level titanium dioxide, No. CAS：13463-67-7, protecting Carter new material purchased from Nanjing has
Limit company, model PTT-T10, Detitanium-ore-type, grain size 5nm.
Vermiculite, purchased from Lingshou County Shi Nuo mineral products processings factory, grain size 2mm.
Nano ferrous iron oxide, i.e., nano level ferrous oxide, No. CAS：1345-25-1 climbs field powder body material purchased from Shanghai
Co., Ltd, grain size 30nm, content 99.9%.
Nano zine oxide, the nano level zinc oxide of grade, No. CAS：1314-13-2, purchased from the Nanjing day limited public affairs of row new material
Department, model TZP-50, grain size 30nm, content 99.9%.
Dried peppermint leaf is the fresh of Labiatae mint peppermint (Classification system Mentha haplocalyx Briq.)
Leaf, place of production Fuyang.Dried peppermint leaf containing peppermint oil, Herba Menthae cream, camphor terpene, lemon terpene, protein, fat, carbohydrate,
The ingredients such as minerals, vitamin, it is full of nutrition.
Pomegranate leaf is pomegranate (Classification system：Punica granatum L.) Fresh leaves, place of production Jiangsu Province Shuyang
County.
Perilla leaf is Labiatae purple perilla platymiscium purple perilla (Classification system：Perilla frutescens(L.)Britt.)
Fresh leaves, place of production Qingyang District, Gansu Province.
White granulated sugar, purchased from the prosperous Kowloon bio tech ltd in Jinan, food-grade.
Lactobacillus plantarum, Classification system：Lactobacillus plantarum, by the bright limited public affairs of moral biotechnology in Xi'an
Department provides, and food-grade, bacterial strain Han Liang≤5.0 × 109cfu/g, Shui divides Han Liang≤6.0%.
Hole tray, purchased from Heng Kun plastic products factory, specification 540mm × 280mm, cave number 72, upper bore 40mm, lower relative aperture
40mm, height 45mm, material PVC.
Ultraviolet lamp, the ultraviolet-sterilization that the wavelength produced using Zhuozhou City rising sun Puri electric light source Manufacturing Co., Ltd is 254nm
Lamp.
Growth cabinet, purchased from Shanghai Zuo Le Instrument Ltd., model PRX350A.
In following embodiments, used test method and key instrument are as follows：
Seed originates rootage duration and rooting rate test：The seed for observing and recording each embodiment daily is taken root situation, note
The starting rootage duration of seed is recorded, until seed urges root culture 40 days, calculates seed rooting rate, seed rooting rate=seed is taken root
Number/seed sum × 100%.
The calculating of percentage of seedgermination：The germination situation of each embodiment is observed and recorded daily, until presprouting of seeds nursery
The 40th day afterwards, calculate percentage of seedgermination, percentage of seedgermination=germination number/seed sum × 100%.
Height of seedling, the trunk diameter of tree peony seedling of each embodiment through step (4) cultivation the 7th day are measured, measurement knot
Fruit takes the average value of 30 plants of tree peony seedlings.
It is further illustrated the present invention below by the mode of embodiment, unless otherwise specified, used number is attached most importance to
Measure number.
Embodiment 1
The method for culturing seedlings of oil Paeonia suffruticosa seed, comprises the following steps：
(1) seed collection and processing：The ripening fruits of mid or late August phoenix pellet tree peony is gathered, is placed on airing at shady and cool ventilation,
When fruit natural cracking, seed is taken out, and ripe seed is selected with wet cleaning process；
(2) seed urges root culture：By seed temperature be 25 DEG C, quality be seed quality three times and mass fraction be
It is impregnated 5 minutes in 5% aqueous sodium hypochlorite solution, seed is taken out after dipping, is rinsed well with clear water, drains the surface of the seed water
Point, seed impregnate in the water that temperature is 25 DEG C, quality is four times of seed quality 9 it is small when, taking-up seed, airing in the cool
6 it is small when, then seed is placed in the hole tray equipped with matrix I, a seed is put in each hole tray, seed buries in matrix I
Depth is 1cm, and hole tray is placed in 20 DEG C of growth cabinet and is cultivated to the root for growing 3.5-3.8cm, wet in growth cabinet
It spends for 75%, daily illumination 12h, flux density 3000lx, dark 12h during culture；
The matrix I is that river sand, plant ash, perlite are 2 in mass ratio:1:1 mixture, hole tray is interior to be packed into matrix I
Volume be hole tray volume 4/5；
(3) presprouting of seeds nursery：The seed for the root for growing 3.5-3.8cm after step (2) culture is placed in equipped with matrix
In II hole tray, a seed is put in each hole tray, the root of seed is imbedded in matrix II, cultivated under natural lighting to seed
Grow two panels true leaf；The matrix II is that river sand, vermiculite are 4 in mass ratio:1 mixture, the interior body for being packed into matrix II of hole tray
Product is the 4/5 of hole tray volume；
(4) transplanting and final-period management：The seedling for growing two panels true leaf is transplanted in sand bed under natural light and is cultivated, uses sunshade
Net shading, it is 40% that daily water drenching, which keeps sand bed humidity,；
Husky thickness 15cm in the sand bed, it is 40cm that seedling, which is transplanted to the seedling spacing after sand bed, and sand used in sand bed is river sand.
The wet cleaning process seed selection is to wash with water seed totally, in the water that temperature is 25 DEG C, quality is 5 times of seed quality
Middle immersion 1h removes sundries waterborne and floating seed, chooses the full seed of sinking, drains the surface of the seed moisture to get to use
In the ripe seed of the present embodiment.
Embodiment 2
The method for culturing seedlings of oil Paeonia suffruticosa seed, comprises the following steps：
(1) seed collection and processing：The ripening fruits of mid or late August phoenix pellet tree peony is gathered, is placed on airing at shady and cool ventilation,
When fruit natural cracking, seed is taken out, and ripe seed is selected with wet cleaning process；
(2) seed urges root culture：By seed temperature be 25 DEG C, quality be seed quality three times and mass fraction be
It impregnates 5 minutes and sterilizes in 5% liquor natrii hypochloritis, take out seed, rinsed well with clear water, drain the surface of the seed moisture, it will
Seed temperature be 25 DEG C, dipping 9 is small in seed-soaking liquid that quality is four times of seed quality when, take out seed, spread out in the cool
Dry in the air 6 it is small when, then seed is placed in the hole tray equipped with matrix I, a seed is put in each hole tray, seed buries in matrix I
Tibetan depth is 1cm, and hole tray is placed in 20 DEG C of growth cabinet and is cultivated to the root for growing 3.5-3.8cm, in growth cabinet
Humidity is 75%, daily illumination 12h, flux density 3000lx, dark 12h during culture；
The matrix I is that river sand, plant ash, perlite are 2 in mass ratio:1:1 mixture, hole tray is interior to be packed into matrix I
Volume be hole tray volume 4/5；The preparation method of the seed-soaking liquid is as follows：By 10mg potassium sulfates, 8mg sulfuric acid, 100mg dimension lifes
Plain B1,30mg thiocarbamide and 30mg sorbic acids are added in the 500g water that temperature is 25 DEG C, and 30 points are stirred with 500 revs/min of rotating speed
Clock obtains seed-soaking liquid；
(3) presprouting of seeds nursery：The seed for the root for growing 3.5-3.8cm after step (2) culture is placed in equipped with matrix
In II hole tray, a seed is put in each hole tray, the root of seed is imbedded in matrix II, cultivated under natural lighting to seed
Grow two panels true leaf；The matrix II is that river sand, vermiculite are 4 in mass ratio:1 mixture, the interior body for being packed into matrix II of hole tray
Product is the 4/5 of hole tray volume；
(4) transplanting and final-period management：The seedling for growing two panels true leaf is transplanted in sand bed under natural light and is cultivated, uses sunshade
Net shading, it is 40% that daily water drenching, which keeps sand bed humidity,；
Husky thickness 15cm in the sand bed, it is 40cm that seedling, which is transplanted to the seedling spacing after sand bed, and sand used in sand bed is river sand.
The step of wet cleaning process seed selection, is the same as embodiment 1.
Embodiment 3
The method for culturing seedlings of oil Paeonia suffruticosa seed, comprises the following steps：
(1) seed collection and processing：The ripening fruits of mid or late August phoenix pellet tree peony is gathered, is placed on airing at shady and cool ventilation,
When fruit natural cracking, seed is taken out, and ripe seed is selected with wet cleaning process；
(2) seed urges root culture：By seed temperature be 25 DEG C, quality be seed quality three times and mass fraction be
It impregnates 5 minutes and sterilizes in 5% liquor natrii hypochloritis, take out seed, rinsed well with clear water, drain the surface of the seed moisture, it will
Seed temperature be 25 DEG C, dipping 9 is small in seed-soaking liquid that quality is four times of seed quality when, take out seed, spread out in the cool
Dry in the air 6 it is small when, then seed is placed in the hole tray equipped with matrix I, a seed is put in each hole tray, seed buries in matrix I
Tibetan depth is 1cm, and hole tray is placed in 20 DEG C of growth cabinet and is cultivated to the root for growing 3.5-3.8cm, in growth cabinet
Humidity is 75%, daily illumination 12h, flux density 3000lx during culture, with wavelength is 254nm after illumination, power 10W
Ultra violet lamp hole tray 45min, ultraviolet lamp is located at directly over hole tray, horizontal positioned, vertical range of the ultraviolet lamp apart from hole tray
For 40 centimetres, remaining time is interlunation；
The matrix I is that river sand, plant ash, perlite are 2 in mass ratio:1:1 mixture, hole tray is interior to be packed into matrix I
Volume be hole tray volume 4/5；The preparation method of the seed-soaking liquid is as follows：By 10mg potassium sulfates, 8mg sulfuric acid, 100mg dimension lifes
Plain B1,30mg thiocarbamide, 0.15mg nano-titanium oxides and 30mg sorbic acids are added to temperature, with 500 turns/
The rotating speed divided stirs 30 minutes, obtains seed-soaking liquid；
(3) presprouting of seeds nursery：The seed for the root for growing 3.5-3.8cm after step (2) culture is placed in equipped with matrix
In II hole tray, a seed is put in each hole tray, the root of seed is imbedded in matrix II, cultivated under natural lighting to seed
Grow two panels true leaf；The matrix II is that river sand, vermiculite are 4 in mass ratio:1 mixture, the interior body for being packed into matrix II of hole tray
Product is the 4/5 of hole tray volume；
(4) transplanting and final-period management：The seedling for growing two panels true leaf is transplanted in sand bed under natural light and is cultivated, uses sunshade
Net shading, it is 40% that daily water drenching, which keeps sand bed humidity,；
Husky thickness 15cm in the sand bed, it is 40cm that seedling, which is transplanted to the seedling spacing after sand bed, and sand used in sand bed is river sand.
The step of wet cleaning process seed selection, is the same as embodiment 1.
Embodiment 4
It is substantially the same manner as Example 3, it differs only in：The seed of step (2) described in the present embodiment 4 urges the root culture to be：It will
Seed impregnates 5 minutes in the liquor natrii hypochloritis that temperature is 25 DEG C, quality is seed quality three times and mass fraction is 5%
Disinfection, take out seed, rinsed well with clear water, drain the surface of the seed moisture, by seed temperature be 25 DEG C, quality be seed
When dipping 9 is small in the seed-soaking liquid that four times of quality, seed is taken out, when airing 6 is small in the cool, then seed is placed in equipped with matrix
In I hole tray, a seed is put in each hole tray, seed buried depth in matrix I is 1cm, and hole tray is placed on to 20 DEG C of people
It is cultivated in work climate box to the root for growing 3.5-3.8cm, humidity is 75%, daily illumination 12h during culture in growth cabinet,
Flux density is 3000lx, with the ultra violet lamp hole tray 25min that wavelength is 254nm, power is 21W, ultraviolet lamp position after illumination
Horizontal positioned directly over hole tray, vertical range of the ultraviolet lamp apart from hole tray is 40 centimetres, and remaining time is interlunation；
The matrix I is that river sand, plant ash, perlite are 2 in mass ratio:1:1 mixture, hole tray is interior to be packed into matrix I
Volume be hole tray volume 4/5；The preparation method of the seed-soaking liquid is as follows：By 10mg potassium sulfates, 8mg sulfuric acid, 100mg dimension lifes
Plain B1,30mg thiocarbamide, 0.3mg nano-titanium oxides and 30mg sorbic acids are added to temperature, with 500 turns/
The rotating speed divided stirs 30 minutes, obtains seed-soaking liquid.
Embodiment 5
It is substantially the same manner as Example 3, it differs only in：The seed of step (2) described in the present embodiment 5 urges the root culture to be：It will
Seed impregnates 5 minutes in the liquor natrii hypochloritis that temperature is 25 DEG C, quality is seed quality three times and mass fraction is 5%
Disinfection, take out seed, rinsed well with clear water, drain the surface of the seed moisture, by seed temperature be 25 DEG C, quality be seed
When dipping 9 is small in the seed-soaking liquid that four times of quality, seed is taken out, when airing 6 is small in the cool, then seed is placed in equipped with matrix
In I hole tray, a seed is put in each hole tray, seed buried depth in matrix I is 1cm, and hole tray is placed on to 20 DEG C of people
It is cultivated in work climate box to the root for growing 3.5-3.8cm, humidity is 75%, daily illumination 12h during culture in growth cabinet,
Flux density is 3000lx, with the ultra violet lamp hole tray 15min that wavelength is 254nm, power is 40W, ultraviolet lamp position after illumination
Horizontal positioned directly over hole tray, vertical range of the ultraviolet lamp apart from hole tray is 40 centimetres, and remaining time is interlunation；
The matrix I is that river sand, plant ash, perlite are 2 in mass ratio:1:1 mixture, hole tray is interior to be packed into matrix I
Volume be hole tray volume 4/5；The preparation method of the seed-soaking liquid is as follows：By 10mg potassium sulfates, 8mg sulfuric acid, 100mg dimension lifes
Plain B1,30mg thiocarbamide, 0.45mg nano-titanium oxides and 30mg sorbic acids are added to temperature, with 500 turns/
The rotating speed divided stirs 30 minutes, obtains seed-soaking liquid.
Embodiment 6
The method for culturing seedlings of oil Paeonia suffruticosa seed, comprises the following steps：
(1) seed collection and processing：The ripening fruits of mid or late August phoenix pellet tree peony is gathered, is placed on airing at shady and cool ventilation,
When fruit natural cracking, seed is taken out, and ripe seed is selected with wet cleaning process；
(2) seed urges root culture：By seed temperature be 25 DEG C, quality be seed quality three times and mass fraction be
It impregnates 5 minutes and sterilizes in 5% liquor natrii hypochloritis, take out seed, rinsed well with clear water, drain the surface of the seed moisture, it will
Seed temperature be 25 DEG C, dipping 9 is small in seed-soaking liquid that quality is four times of seed quality when, take out seed, spread out in the cool
Dry in the air 6 it is small when, then seed is placed in the hole tray equipped with matrix I, a seed is put in each hole tray, seed buries in matrix I
Tibetan depth is 1cm, and hole tray is placed in 20 DEG C of growth cabinet and is cultivated to the root for growing 3.5-3.8cm, in growth cabinet
Humidity is 75%, daily illumination 12h, flux density 3000lx during culture, with wavelength is 254nm after illumination, power 21W
Ultra violet lamp hole tray 25min, ultraviolet lamp is located at directly over hole tray, horizontal positioned, vertical range of the ultraviolet lamp apart from hole tray
For 40 centimetres, remaining time is interlunation；
The matrix I is that river sand, plant ash, perlite are 2 in mass ratio:1:1 mixture, hole tray is interior to be packed into matrix I
Volume be hole tray volume 4/5；The preparation method of the seed-soaking liquid is as follows：By 10mg potassium sulfates, 8mg sulfuric acid, 100mg dimension lifes
Plain B1,30mg thiocarbamide, 0.3mg nano-titanium oxides and 30mg sorbic acids are added to temperature, with 500 turns/
The rotating speed divided stirs 30 minutes, obtains seed-soaking liquid；
(3) presprouting of seeds nursery：The seed for the root for growing 3.5-3.8cm after step (2) culture is placed in equipped with matrix
In II hole tray, a seed is put in each hole tray, the root of seed is imbedded in matrix II, the middle spray into each hole tray daily
5 milliliters of nutrient solution is spilt, culture to seed grows two panels true leaf under natural lighting；The matrix II is river sand, vermiculite presses matter
Amount is than being 4:1 mixture, the interior volume for being packed into matrix II of hole tray is the 4/5 of hole tray volume；The preparation method of the nutrient solution
As follows, described part is parts by weight：Dried peppermint leaf, pomegranate leaf, perilla leaf be crushed into 200 mesh sieves respectively, take 50 parts after crushing
Dried peppermint leaf, 30 parts of pomegranate leafs, 15 parts of perilla leafs and 5 portions of white granulated sugars, 1 part of lactobacillus plantarum, 60 parts of water are added to after mixing
In fermentation tank, 35 DEG C are sealed by fermentation 20 days, and the mixture after fermentation is crossed 300 mesh sieves, retain filtrate to get nutrient solution；
(4) transplanting and final-period management：The seedling for growing two panels true leaf is transplanted in sand bed under natural light and is cultivated, uses sunshade
Net shading, it is 40% that daily water drenching, which keeps sand bed humidity,；
Husky thickness 15cm in the sand bed, it is 40cm that seedling, which is transplanted to the seedling spacing after sand bed, and sand used in sand bed is river sand.
The step of wet cleaning process seed selection, is the same as embodiment 1.
Embodiment 7
It is substantially the same manner as Example 6, it differs only in：The preparation method of nutrient solution is as follows described in the present embodiment 7, described
Part is parts by weight：Dried peppermint leaf, pomegranate leaf, perilla leaf be crushed into 200 mesh sieves respectively, take 40 parts of dried peppermint leaves, 35 after crushing
Part pomegranate leaf, 20 parts of perilla leafs and 5 portions of white granulated sugars, 1 part of lactobacillus plantarum, 60 parts of water are added in fermentation tank after mixing,
30 DEG C are sealed by fermentation 25 days, and the mixture after fermentation is crossed 300 mesh sieves, retain filtrate to get nutrient solution.
Embodiment 8
It is substantially the same manner as Example 6, it differs only in：The preparation method of nutrient solution is as follows described in the present embodiment 8, described
Part is parts by weight：Dried peppermint leaf, pomegranate leaf, perilla leaf be crushed into 200 mesh sieves respectively, take 25 parts of dried peppermint leaves, 25 after crushing
Part pomegranate leaf, 45 parts of perilla leafs and 5 portions of white granulated sugars, 1 part of lactobacillus plantarum, 60 parts of water are added in fermentation tank after mixing,
20 DEG C are sealed by fermentation 40 days, and the mixture after fermentation is crossed 300 mesh sieves, retain filtrate to get nutrient solution.
Embodiment 9
The method for culturing seedlings of oil Paeonia suffruticosa seed, comprises the following steps：
(1) seed collection and processing：The ripening fruits of mid or late August phoenix pellet tree peony is gathered, is placed on airing at shady and cool ventilation,
When fruit natural cracking, seed is taken out, and ripe seed is selected with wet cleaning process；
(2) seed urges root culture：By seed temperature be 25 DEG C, quality be seed quality three times and mass fraction be
It impregnates 5 minutes and sterilizes in 5% liquor natrii hypochloritis, take out seed, rinsed well with clear water, drain the surface of the seed moisture, it will
Seed temperature be 25 DEG C, dipping 9 is small in seed-soaking liquid that quality is four times of seed quality when, take out seed, spread out in the cool
Dry in the air 6 it is small when, then seed is placed in the hole tray equipped with matrix I, a seed is put in each hole tray, seed buries in matrix I
Tibetan depth is 1cm, and hole tray is placed in 20 DEG C of growth cabinet and is cultivated to the root for growing 3.5-3.8cm, in growth cabinet
Humidity is 75%, daily illumination 12h, flux density 3000lx during culture, with wavelength is 254nm after illumination, power 21W
Ultra violet lamp hole tray 25min, ultraviolet lamp is located at directly over hole tray, horizontal positioned, vertical range of the ultraviolet lamp apart from hole tray
For 40 centimetres, remaining time is interlunation；
The matrix I is that river sand, plant ash, perlite are 2 in mass ratio:1:1 mixture, hole tray is interior to be packed into matrix I
Volume be hole tray volume 4/5；The preparation method of the seed-soaking liquid is as follows：By 10mg potassium sulfates, 8mg sulfuric acid, 100mg dimension lifes
Plain B1,30mg thiocarbamide, 0.3mg nano-titanium oxides and 30mg sorbic acids are added to temperature, with 500 turns/
The rotating speed divided stirs 30 minutes, obtains seed-soaking liquid；
(3) presprouting of seeds nursery：The seed for the root for growing 3.5-3.8cm after step (2) culture is placed in equipped with matrix
In II hole tray, a seed is put in each hole tray, the root of seed is imbedded in matrix II, the middle spray into each hole tray daily
5 milliliters of nutrient solution is spilt, culture to seed grows two panels true leaf under natural lighting；The matrix II is river sand, vermiculite, nanometer
Material is 4 in mass ratio:1：0.02 mixture, the interior volume for being packed into matrix II of hole tray is the 4/5 of hole tray volume；The nanometer
Material is nano ferrous iron oxide；The preparation method of the nutrient solution is as follows, and described part is parts by weight：By dried peppermint leaf, pomegranate leaf,
Perilla leaf crushed 200 mesh sieves respectively, take 40 parts of dried peppermint leaves, 35 parts of pomegranate leafs, 20 parts of perilla leafs and 5 parts of white sands after crushing
Sugar, 1 part of lactobacillus plantarum, 60 parts of water are added in fermentation tank after mixing, and 30 DEG C are sealed by fermentation 25 days, will be mixed after fermentation
It closes object and crosses 300 mesh sieves, retain filtrate to get nutrient solution；
(4) transplanting and final-period management：The seedling for growing two panels true leaf is transplanted in sand bed under natural light and is cultivated, uses sunshade
Net shading, it is 40% that daily water drenching, which keeps sand bed humidity,；
Husky thickness 15cm in the sand bed, it is 40cm that seedling, which is transplanted to the seedling spacing after sand bed, and sand used in sand bed is river sand.
The step of wet cleaning process seed selection, is the same as embodiment 1.
Embodiment 10
It is substantially the same manner as Example 9, it differs only in, nano material described in the present embodiment 10 is nano zine oxide.
Embodiment 11
It is substantially the same manner as Example 9, differ only in, nano material described in the present embodiment 11 for nano ferrous iron oxide and
Nano zine oxide is 3 in mass ratio:1 mixture.
The germination percentage test result of the present embodiment 11 is：95.3%, to the tree peony seedling after transplanting and final-period management 7 days
Height of seedling and trunk diameter measurement are carried out, height of seedling and trunk diameter are respectively 12.2cm, 1.5cm.
Effect example 1
The situation of taking root of each embodiment is observed, rooting rate and starting rootage duration are recorded and calculated, the results are shown in Table
1。
Table 1：Originate rootage duration and rooting rate result table
| Originate rootage duration/day | Rooting rate/% | |
| Embodiment 1 | 26 | 76.2 |
| Embodiment 2 | 22 | 82.1 |
| Embodiment 3 | 17 | 87.6 |
| Embodiment 4 | 15 | 93.5 |
| Embodiment 5 | 19 | 88.4 |
Comparing embodiment 2 and embodiment 1, seed are impregnated in the seed-soaking liquid of embodiment 2, than impregnating in water more conducively
Seed is taken root.Compared with Example 2, also contain nano-titanium oxide in the seed-soaking liquid of embodiment 3-5, above-mentioned seed-soaking liquid will be impregnated
Seed be aided with ultraviolet illumination, the cell and tissue of nano-titanium oxide energy activated seed activate seed enzymatic activity, and ultraviolet light shines
Penetrating can excite seed to take root vigor, and promotion makes intraseminal Free water become combining water.Seed-soaking liquid component proportion in embodiment 3
And ultraviolet light irradiation condition is more conducive to seed and takes root.
Effect example 2
The germination of each embodiment is observed, germination percentage is counted, the results are shown in Table 2.
Table 2：Germination percentage test result table
| Germination percentage/% | |
| Embodiment 4 | 71.5 |
| Embodiment 6 | 70.2 |
| Embodiment 7 | 83.1 |
| Embodiment 8 | 81.4 |
| Embodiment 9 | 90.7 |
| Embodiment 10 | 87.6 |
Compared with Example 4, embodiment 6-8 sprays nutrient solution, this hair into the matrix II containing seed of having taken root daily
The bright nutrient solution used tunning for selecting dried peppermint leaf, pomegranate leaf and perilla leaf, embodiment 6-8 is by optimizing dried peppermint leaf, pomegranate
The ratio and fermentation condition of leaf and perilla leaf have obtained the nutrient solution of more conducively germination.The nutrition that wherein embodiment 7 uses
Liquid has the effect of better stratification.
Respectively containing nano ferrous iron oxide and nano zine oxide in embodiment 9, the matrix II of embodiment 10, addition is above-mentioned to be received
Rice material after, the germination percentage of seed significantly improves, it may be possible to nano material by seed root system absorb after, by with cell membrane and life
The combination of object macromolecular influences the breeding of cell, promotes the germination of seed.
Effect example 3
Height of seedling and trunk diameter measurement, measurement are carried out to tree peony seedling of each embodiment after transplanting and final-period management 7 days
As a result table 3 between.
Table 3：Height of seedling and trunk diameter test result table
| Height of seedling/cm | Trunk diameter/cm | |
| Embodiment 4 | 3.8 | 0.3 |
| Embodiment 6 | 5.1 | 0.5 |
| Embodiment 7 | 6.6 | 0.7 |
| Embodiment 8 | 5.4 | 0.6 |
| Embodiment 9 | 9.5 | 1.2 |
| Embodiment 10 | 9.0 | 1.0 |
The tree peony seedling height of seedling of 30 days that embodiment 6-8 method for culturing seedlings obtains is urged apparently higher than embodiment 4, sprinkling nutrient solution
It is transplanted after the germination of bud, seedling early growth is faster.It is used in pregermination procedure containing nano ferrous iron oxide and nano zine oxide
Matrix (embodiment 9, embodiment 10), the seed after germination is transplanted again, and seedling early growth is fast, and trunk length is thick apparent, cultivation
Seedling is healthier, more has vitality.
The preferred embodiment of the present invention described in detail above.It should be appreciated that those of ordinary skill in the art without
Creative work is needed according to the present invention can to conceive and makes many modifications and variations.Therefore, all technologies in the art
Personnel are available by logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Technical solution, all should be in the protection domain being defined in the patent claims.
Claims (8)
1. a kind of method for culturing seedlings of oil Paeonia suffruticosa seed, which is characterized in that comprise the following steps：
(1) seed collection and processing：The ripening fruits of mid or late August phoenix pellet tree peony is gathered, airing at shady and cool ventilation is placed on, treats fruit
During real natural cracking, seed is taken out, and ripe seed is selected with wet cleaning process；
(2) seed urges root culture：It will be rinsed well after seed disinfection with clear water, the surface of the seed moisture drained, by seed in 25-35
DEG C, quality for 4-6 times of seed quality water in impregnate 9-15 it is small when, take out seed, when airing 6-12 is small in the cool, then general
Seed is placed in the hole tray equipped with matrix I, and a seed is put in each hole tray, and hole tray is placed on to 20-30 DEG C of growth cabinet
Middle to cultivate the root that more than 3cm is grown to seed, humidity is 75-90%, daily illumination 12h during culture in growth cabinet, is led to
Metric density is 3000-12000lx, dark 12h；
The matrix I is that river sand, plant ash, perlite are 2 in mass ratio:1:1 mixture；
(3) presprouting of seeds nursery：The seed for the root for growing more than 3cm after step (2) culture is placed in the cave equipped with matrix II
In disk, a seed is put in each hole tray, culture to seed grows two panels true leaf under natural lighting；The matrix II for river sand,
Vermiculite is 4 in mass ratio:1 mixture；
(4) transplanting and final-period management：The seedling for growing two panels true leaf is transplanted in sand bed under natural light and is cultivated, is hidden with sunshade net
The moon, it is 40-60% that daily water drenching, which keeps sand bed humidity,；Husky thickness 15-17cm in the sand bed, seedling are transplanted between the seedling after sand bed
Away from for 35-55cm, sand used in sand bed is river sand.
2. a kind of method for culturing seedlings of oil Paeonia suffruticosa seed, which is characterized in that comprise the following steps：
(1) seed collection and processing：The ripening fruits of mid or late August phoenix pellet tree peony is gathered, airing at shady and cool ventilation is placed on, treats fruit
During real natural cracking, seed is taken out, and ripe seed is selected with wet cleaning process；
(2) seed urges root culture：It will be rinsed well after seed disinfection with clear water, the surface of the seed moisture drained, by seed in 25-35
DEG C, quality for 4-6 times of seed quality seed-soaking liquid in impregnate 9-15 it is small when, taking-up seed, when airing 6-12 is small in the cool, so
Seed is placed in the hole tray equipped with matrix I afterwards, a seed is put in each hole tray, hole tray is placed on to 20-30 DEG C of artificial gas
It waits culture in case and grows the root of more than 3cm to seed, humidity is 75-90% in growth cabinet, daily illumination during culture
12h, flux density 3000-12000lx, dark 12h；
The matrix I is that river sand, plant ash, perlite are 2 in mass ratio:1:1 mixture；The preparation method of the seed-soaking liquid
It is as follows：By 10-40mg potassium sulfates, 8-32mg sulfuric acid, 100-200mg vitamin B1s, 30-70mg thiocarbamides and 30-70mg sorbic acids
It is added in the 500-1000g water that temperature is 25-50 DEG C, is stirred 30-60 minutes, soaked with 500-1000 revs/min of rotating speed
Kind liquid；
(3) presprouting of seeds nursery：The seed for the root for growing more than 3cm after step (2) culture is placed in the cave equipped with matrix II
In disk, a seed is put in each hole tray, culture to seed grows two panels true leaf under natural lighting；The matrix II for river sand,
Vermiculite is 4 in mass ratio:1 mixture；
(4) transplanting and final-period management：The seedling for growing two panels true leaf is transplanted in sand bed under natural light and is cultivated, is hidden with sunshade net
The moon, it is 40-60% that daily water drenching, which keeps sand bed humidity,；Husky thickness 15-17cm in the sand bed, seedling are transplanted between the seedling after sand bed
Away from for 35-55cm, sand used in sand bed is river sand.
3. a kind of method for culturing seedlings of oil Paeonia suffruticosa seed, which is characterized in that comprise the following steps：
(1) seed collection and processing：The ripening fruits of mid or late August phoenix pellet tree peony is gathered, airing at shady and cool ventilation is placed on, treats fruit
During real natural cracking, seed is taken out, and ripe seed is selected with wet cleaning process；
(2) seed urges root culture：It will be rinsed well after seed disinfection with clear water, the surface of the seed moisture drained, by seed in 25-35
DEG C, quality for 4-6 times of seed quality seed-soaking liquid in impregnate 9-15 it is small when, taking-up seed, when airing 6-12 is small in the cool, so
Seed is placed in the hole tray equipped with matrix I afterwards, a seed is put in each hole tray, hole tray is placed on to 20-30 DEG C of artificial gas
It waits culture in case and grows the root of more than 3cm to seed, humidity is 75-90% in growth cabinet, daily illumination during culture
12h, flux density 3000-12000lx, with the ultra violet lamp cave that wavelength is 254-395nm, power is 10-40W after illumination
Disk 15-45min, ultraviolet lamp is located at directly over hole tray, horizontal positioned, and vertical range of the ultraviolet lamp apart from hole tray is 40-80 centimetres,
Remaining time is interlunation；
The matrix I is that river sand, plant ash, perlite are 2 in mass ratio:1:1 mixture；The preparation method of the seed-soaking liquid
It is as follows：By 10-40mg potassium sulfates, 8-32mg sulfuric acid, 100-200mg vitamin B1s, 30-70mg thiocarbamides, 0.15-1.5mg nanometers
Titanium oxide and 30-70mg sorbic acids are added in the 500-1000g water that temperature is 25-50 DEG C, with 500-1000 revs/min of rotating speed
Stirring 30-60 minutes, obtains seed-soaking liquid；
(3) presprouting of seeds nursery：The seed for the root for growing more than 3cm after step (2) culture is placed in the cave equipped with matrix II
In disk, a seed is put in each hole tray, culture to seed grows two panels true leaf under natural lighting；The matrix II for river sand,
Vermiculite is 4 in mass ratio:1 mixture；
(4) transplanting and final-period management：The seedling for growing two panels true leaf is transplanted in sand bed under natural light and is cultivated, is hidden with sunshade net
The moon, it is 40-60% that daily water drenching, which keeps sand bed humidity,；Husky thickness 15-17cm in the sand bed, seedling are transplanted between the seedling after sand bed
Away from for 35-55cm, sand used in sand bed is river sand.
4. a kind of method for culturing seedlings of oil Paeonia suffruticosa seed, which is characterized in that comprise the following steps：
(1) seed collection and processing：The ripening fruits of mid or late August phoenix pellet tree peony is gathered, airing at shady and cool ventilation is placed on, treats fruit
During real natural cracking, seed is taken out, and ripe seed is selected with wet cleaning process；
(2) seed urges root culture：It will be rinsed well after seed disinfection with clear water, the surface of the seed moisture drained, by seed in 25-35
DEG C, quality for 4-6 times of seed quality seed-soaking liquid in impregnate 9-15 it is small when, taking-up seed, when airing 6-12 is small in the cool, so
Seed is placed in the hole tray equipped with matrix I afterwards, a seed is put in each hole tray, hole tray is placed on to 20-30 DEG C of artificial gas
It waits culture in case and grows the root of more than 3cm to seed, humidity is 75-90% in growth cabinet, daily illumination during culture
12h, flux density 3000-12000lx, with the ultra violet lamp cave that wavelength is 254-395nm, power is 10-40W after illumination
Disk 15-45min, ultraviolet lamp is located at directly over hole tray, horizontal positioned, and vertical range of the ultraviolet lamp apart from hole tray is 40-80 centimetres,
Remaining time is interlunation；
The matrix I is that river sand, plant ash, perlite are 2 in mass ratio:1:1 mixture；The preparation method of the seed-soaking liquid
It is as follows：By 10-40mg potassium sulfates, 8-32mg sulfuric acid, 100-200mg vitamin B1s, 30-70mg thiocarbamides, 0.15-1.5mg nanometers
Titanium oxide and 30-70mg sorbic acids are added in the 500-1000g water that temperature is 25-50 DEG C, with 500-1000 revs/min of rotating speed
Stirring 30-60 minutes, obtains seed-soaking liquid；
(3) presprouting of seeds nursery：The seed for the root for growing more than 3cm after step (2) culture is placed in the cave equipped with matrix II
In disk, a seed is put in each hole tray, daily the middle nutrient solution for spraying 50-200 milliliters into each hole tray, in natural lighting
Lower culture to seed grows two panels true leaf；
The preparation method of the nutrient solution is as follows, and described part is parts by weight：Dried peppermint leaf, pomegranate leaf, perilla leaf are crushed respectively
Cross 200 mesh sieves, take crush after 25-50 parts dried peppermint leaf, 25-35 parts of pomegranate leafs, 15-45 parts of perilla leafs and 5-10 portions of white granulated sugars,
1-5 parts of lactobacillus plantarums, 60-100 parts of water are added in fermentation tank after mixing, and 20-35 DEG C is sealed by fermentation 20-40 days, will
Mixture after fermentation crosses 300 mesh sieves, retains filtrate to get nutrient solution；
The matrix II is that river sand, vermiculite are 4 in mass ratio:1 mixture；
(4) transplanting and final-period management：The seedling for growing two panels true leaf is transplanted in sand bed under natural light and is cultivated, is hidden with sunshade net
The moon, it is 40-60% that daily water drenching, which keeps sand bed humidity,；Husky thickness 15-17cm in the sand bed, seedling are transplanted between the seedling after sand bed
Away from for 35-55cm, sand used in sand bed is river sand.
5. a kind of method for culturing seedlings of oil Paeonia suffruticosa seed, which is characterized in that comprise the following steps：
(1) seed collection and processing：The ripening fruits of mid or late August phoenix pellet tree peony is gathered, airing at shady and cool ventilation is placed on, treats fruit
During real natural cracking, seed is taken out, and ripe seed is selected with wet cleaning process；
(2) seed urges root culture：It will be rinsed well after seed disinfection with clear water, the surface of the seed moisture drained, by seed in 25-35
DEG C, quality for 4-6 times of seed quality seed-soaking liquid in impregnate 9-15 it is small when, taking-up seed, when airing 6-12 is small in the cool, so
Seed is placed in the hole tray equipped with matrix I afterwards, a seed is put in each hole tray, hole tray is placed on to 20-30 DEG C of artificial gas
It waits culture in case and grows the root of more than 3cm to seed, humidity is 75-90% in growth cabinet, daily illumination during culture
12h, flux density 3000-12000lx, with the ultra violet lamp cave that wavelength is 254-395nm, power is 10-40W after illumination
Disk 15-45min, ultraviolet lamp is located at directly over hole tray, horizontal positioned, and vertical range of the ultraviolet lamp apart from hole tray is 40-80 centimetres,
Remaining time is interlunation；
The matrix I is that river sand, plant ash, perlite are 2 in mass ratio:1:1 mixture；The preparation method of the seed-soaking liquid
It is as follows：By 10-40mg potassium sulfates, 8-32mg sulfuric acid, 100-200mg vitamin B1s, 30-70mg thiocarbamides, 0.15-1.5mg nanometers
Titanium oxide and 30-70mg sorbic acids are added in the 500-1000g water that temperature is 25-50 DEG C, with 500-1000 revs/min of rotating speed
Stirring 30-60 minutes, obtains seed-soaking liquid；
(3) presprouting of seeds nursery：The seed for the root for growing more than 3cm after step (2) culture is placed in the cave equipped with matrix II
In disk, a seed is put in each hole tray, daily the middle nutrient solution for spraying 50-200 milliliters into each hole tray, in natural lighting
Lower culture to seed grows two panels true leaf；
The preparation method of the nutrient solution is as follows, and described part is parts by weight：Dried peppermint leaf, pomegranate leaf, perilla leaf are crushed respectively
Cross 200 mesh sieves, take crush after 25-50 parts dried peppermint leaf, 25-35 parts of pomegranate leafs, 15-45 parts of perilla leafs and 5-10 portions of white granulated sugars,
1-5 parts of lactobacillus plantarums, 60-100 parts of water are added in fermentation tank after mixing, and 20-35 DEG C is sealed by fermentation 20-40 days, will
Mixture after fermentation crosses 300 mesh sieves, retains filtrate to get nutrient solution；
The matrix II is that river sand, vermiculite, nano material are 4 in mass ratio:1：0.02 mixture；
The nano material is nano ferrous iron oxide and/or nano zine oxide；
(4) transplanting and final-period management：The seedling for growing two panels true leaf is transplanted in sand bed under natural light and is cultivated, is hidden with sunshade net
The moon, it is 40-60% that daily water drenching, which keeps sand bed humidity,；Husky thickness 15-17cm in the sand bed, seedling are transplanted between the seedling after sand bed
Away from for 35-55cm, sand used in sand bed is river sand.
6. such as the method for culturing seedlings of claim 1-5 any one of them oil Paeonia suffruticosa seeds, which is characterized in that the disinfection is will
Seed impregnates 5 minutes in the liquor natrii hypochloritis that 25 DEG C, quality are 3-6 times of seed quality and mass fraction is 5%.
7. such as the method for culturing seedlings of claim 1-5 any one of them oil Paeonia suffruticosa seeds, which is characterized in that the hole tray is built-in
The volume for entering matrix I is the 4/5 of hole tray volume.
8. such as the method for culturing seedlings of claim 1-5 any one of them oil Paeonia suffruticosa seeds, which is characterized in that the hole tray is built-in
The volume for entering matrix II is the 4/5 of hole tray volume.
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| CN116584293A (en) * | 2023-03-16 | 2023-08-15 | 中国长江三峡集团有限公司 | Method for efficiently breeding eucommia ulmoides in low-altitude area |
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