CN107937422A - 一种panD突变基因、基因工程及其在催化生产β‑丙氨酸中的应用 - Google Patents
一种panD突变基因、基因工程及其在催化生产β‑丙氨酸中的应用 Download PDFInfo
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- C12N9/88—Lyases (4.)
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12P13/00—Preparation of nitrogen-containing organic compounds
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- C12Y401/00—Carbon-carbon lyases (4.1)
- C12Y401/01—Carboxy-lyases (4.1.1)
- C12Y401/01011—Aspartate 1-decarboxylase (4.1.1.11)
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Abstract
Description
Km | Kcat | Vmax(nkcat*mg-1*protein) | Kcat/Km(s-1*mM-1) | |
对照 | 57.49 | 0.002 | 0.126 | 3.5*10-5 |
56号 | 11.48 | 0.0014 | 0.088 | 1.22*10-4 |
134号 | 1.03 | 0.00097 | 0.061 | 9.4*10-4 |
Claims (10)
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108546697A (zh) * | 2018-04-08 | 2018-09-18 | 浙江华睿生物技术有限公司 | 酶法制备beta丙氨酸 |
CN109402097A (zh) * | 2018-10-23 | 2019-03-01 | 江南大学 | 一种L-天冬氨酸α-脱羧酶的基因工程菌株以及应用 |
CN109735522A (zh) * | 2018-12-26 | 2019-05-10 | 浙江工业大学 | 一种L-天冬氨酸-α-脱羧酶突变体及其应用 |
CN113444712A (zh) * | 2021-05-26 | 2021-09-28 | 浙江工业大学 | 一种L-天冬氨酸-α-脱羧酶突变体及其应用 |
Citations (1)
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CN106754845A (zh) * | 2016-11-30 | 2017-05-31 | 浙江工业大学 | 一种panD突变基因、编码蛋白、载体、工程菌及其应用 |
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CN106754845A (zh) * | 2016-11-30 | 2017-05-31 | 浙江工业大学 | 一种panD突变基因、编码蛋白、载体、工程菌及其应用 |
Non-Patent Citations (2)
Title |
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沈艳: "谷氨酸棒杆菌L-天冬氨酸α-脱羧酶催化性能的定点突变分子改造", 《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》 * |
石增秀等: "谷氨酸棒杆菌L-天冬氨酸α-脱羧酶基因的克隆及重组酶性质研究", 《生物技术通报》 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108546697A (zh) * | 2018-04-08 | 2018-09-18 | 浙江华睿生物技术有限公司 | 酶法制备beta丙氨酸 |
CN108546697B (zh) * | 2018-04-08 | 2020-07-24 | 浙江华睿生物技术有限公司 | 酶法制备beta丙氨酸 |
CN109402097A (zh) * | 2018-10-23 | 2019-03-01 | 江南大学 | 一种L-天冬氨酸α-脱羧酶的基因工程菌株以及应用 |
CN109735522A (zh) * | 2018-12-26 | 2019-05-10 | 浙江工业大学 | 一种L-天冬氨酸-α-脱羧酶突变体及其应用 |
CN113444712A (zh) * | 2021-05-26 | 2021-09-28 | 浙江工业大学 | 一种L-天冬氨酸-α-脱羧酶突变体及其应用 |
CN113444712B (zh) * | 2021-05-26 | 2022-06-21 | 浙江工业大学 | 一种L-天冬氨酸-α-脱羧酶突变体及其应用 |
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