CN107857826B - 一种降血糖香蕉花多糖的分离纯化方法 - Google Patents
一种降血糖香蕉花多糖的分离纯化方法 Download PDFInfo
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- CN107857826B CN107857826B CN201711234553.3A CN201711234553A CN107857826B CN 107857826 B CN107857826 B CN 107857826B CN 201711234553 A CN201711234553 A CN 201711234553A CN 107857826 B CN107857826 B CN 107857826B
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- polysaccharide
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- banana flower
- banana
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- 235000018290 Musa x paradisiaca Nutrition 0.000 title claims abstract description 155
- 150000004676 glycans Chemical class 0.000 title claims abstract description 155
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 155
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 155
- 239000008280 blood Substances 0.000 title claims abstract description 28
- 210000004369 blood Anatomy 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000000746 purification Methods 0.000 title claims abstract description 13
- 238000000926 separation method Methods 0.000 title claims abstract description 13
- 240000005561 Musa balbisiana Species 0.000 title 1
- 241000234295 Musa Species 0.000 claims abstract description 155
- 239000000284 extract Substances 0.000 claims abstract description 68
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 11
- 239000012528 membrane Substances 0.000 claims abstract description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 75
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 54
- 238000000605 extraction Methods 0.000 claims description 53
- 239000003208 petroleum Substances 0.000 claims description 52
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 48
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 45
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 33
- 238000003810 ethyl acetate extraction Methods 0.000 claims description 32
- 239000002244 precipitate Substances 0.000 claims description 32
- 239000000126 substance Substances 0.000 claims description 26
- 238000004062 sedimentation Methods 0.000 claims description 18
- 239000008367 deionised water Substances 0.000 claims description 16
- 229910021641 deionized water Inorganic materials 0.000 claims description 16
- 238000001914 filtration Methods 0.000 claims description 16
- 239000000843 powder Substances 0.000 claims description 16
- 239000006228 supernatant Substances 0.000 claims description 16
- 239000002021 butanolic extract Substances 0.000 claims description 9
- 239000012259 ether extract Substances 0.000 claims description 9
- 239000002024 ethyl acetate extract Substances 0.000 claims description 9
- 241001164374 Calyx Species 0.000 claims description 8
- 238000009835 boiling Methods 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 8
- 239000000706 filtrate Substances 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 238000007873 sieving Methods 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- 230000002218 hypoglycaemic effect Effects 0.000 abstract description 11
- 238000011160 research Methods 0.000 abstract description 8
- 238000011161 development Methods 0.000 abstract description 3
- HIXDQWDOVZUNNA-UHFFFAOYSA-N 2-(3,4-dimethoxyphenyl)-5-hydroxy-7-methoxychromen-4-one Chemical compound C=1C(OC)=CC(O)=C(C(C=2)=O)C=1OC=2C1=CC=C(OC)C(OC)=C1 HIXDQWDOVZUNNA-UHFFFAOYSA-N 0.000 description 38
- 102100024295 Maltase-glucoamylase Human genes 0.000 description 9
- 108010028144 alpha-Glucosidases Proteins 0.000 description 9
- 239000000463 material Substances 0.000 description 6
- 238000000338 in vitro Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- XUFXOAAUWZOOIT-SXARVLRPSA-N (2R,3R,4R,5S,6R)-5-[[(2R,3R,4R,5S,6R)-5-[[(2R,3R,4S,5S,6R)-3,4-dihydroxy-6-methyl-5-[[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)-1-cyclohex-2-enyl]amino]-2-oxanyl]oxy]-3,4-dihydroxy-6-(hydroxymethyl)-2-oxanyl]oxy]-6-(hydroxymethyl)oxane-2,3,4-triol Chemical compound O([C@H]1O[C@H](CO)[C@H]([C@@H]([C@H]1O)O)O[C@H]1O[C@@H]([C@H]([C@H](O)[C@H]1O)N[C@@H]1[C@@H]([C@@H](O)[C@H](O)C(CO)=C1)O)C)[C@@H]1[C@@H](CO)O[C@@H](O)[C@H](O)[C@H]1O XUFXOAAUWZOOIT-SXARVLRPSA-N 0.000 description 2
- 229940077274 Alpha glucosidase inhibitor Drugs 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 229960002632 acarbose Drugs 0.000 description 2
- XUFXOAAUWZOOIT-UHFFFAOYSA-N acarviostatin I01 Natural products OC1C(O)C(NC2C(C(O)C(O)C(CO)=C2)O)C(C)OC1OC(C(C1O)O)C(CO)OC1OC1C(CO)OC(O)C(O)C1O XUFXOAAUWZOOIT-UHFFFAOYSA-N 0.000 description 2
- 239000003888 alpha glucosidase inhibitor Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 239000006286 aqueous extract Substances 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 229930003944 flavone Natural products 0.000 description 2
- 235000011949 flavones Nutrition 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000000291 postprandial effect Effects 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 210000000813 small intestine Anatomy 0.000 description 2
- 238000011895 specific detection Methods 0.000 description 2
- 238000003809 water extraction Methods 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000004366 Glucosidases Human genes 0.000 description 1
- 108010056771 Glucosidases Proteins 0.000 description 1
- 208000013016 Hypoglycemia Diseases 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000003472 antidiabetic agent Substances 0.000 description 1
- 235000021015 bananas Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000023852 carbohydrate metabolic process Effects 0.000 description 1
- 235000021256 carbohydrate metabolism Nutrition 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000002026 chloroform extract Substances 0.000 description 1
- 210000004292 cytoskeleton Anatomy 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000000469 ethanolic extract Substances 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 125000000185 sucrose group Chemical group 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
Description
样品 | IC<sub>50</sub>/ug·mL<sup>-1</sup> |
总提取物 | 1726.3 |
石油醚萃取物 | 2635.8 |
乙酸乙酯萃取物 | 2108.4 |
正丁醇萃取物 | 1824.1 |
水萃取物 | 742.5 |
阿卡波糖(阳性对照) | 677.4 |
样品 | IC<sub>50</sub>/ug·mL<sup>-1</sup> |
香蕉花粗多糖溶液 | 726.3 |
Mr>30kD的多糖溶液 | 235.8 |
10kD<Mr<30kD的多糖溶液 | 875.9 |
3kD<Mr<10kD的多糖溶液 | 824.1 |
650D<Mr<3kD的多糖溶液 | 908.4 |
Mr<650D的多糖溶液 | 1045.5 |
阿卡波糖(阳性对照) | 682.3 |
Claims (1)
Priority Applications (1)
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CN201711234553.3A CN107857826B (zh) | 2017-11-30 | 2017-11-30 | 一种降血糖香蕉花多糖的分离纯化方法 |
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CN201711234553.3A CN107857826B (zh) | 2017-11-30 | 2017-11-30 | 一种降血糖香蕉花多糖的分离纯化方法 |
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CN107857826A CN107857826A (zh) | 2018-03-30 |
CN107857826B true CN107857826B (zh) | 2020-12-11 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111234046B (zh) * | 2020-03-23 | 2021-10-15 | 广西壮族自治区农业科学院 | 茉莉花渣活性多糖的制备方法及其应用 |
CN115353571A (zh) * | 2022-08-05 | 2022-11-18 | 广西民族大学 | 一种从香蕉花中提取降血糖香蕉花多糖的方法 |
CN116621999A (zh) * | 2022-11-26 | 2023-08-22 | 中国热带农业科学院海口实验站 | 一种香蕉花多糖的提取及其对晚期糖基化终末产物的抑制作用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1397545A (zh) * | 2002-08-14 | 2003-02-19 | 鹰翰国际贸易(上海)有限公司 | 一种从胡芦巴种子中提取4-羟基-异亮氨酸及胡芦巴胶等多种副产品的方法 |
CN102133247A (zh) * | 2011-01-28 | 2011-07-27 | 西南大学 | 具有降血糖活性的木香提取物及其应用 |
WO2014117238A1 (pt) * | 2013-02-01 | 2014-08-07 | Universidade Federal Do Paraná | Extratos e frações de musa paradisiaca l. e seus usos |
-
2017
- 2017-11-30 CN CN201711234553.3A patent/CN107857826B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1397545A (zh) * | 2002-08-14 | 2003-02-19 | 鹰翰国际贸易(上海)有限公司 | 一种从胡芦巴种子中提取4-羟基-异亮氨酸及胡芦巴胶等多种副产品的方法 |
CN102133247A (zh) * | 2011-01-28 | 2011-07-27 | 西南大学 | 具有降血糖活性的木香提取物及其应用 |
WO2014117238A1 (pt) * | 2013-02-01 | 2014-08-07 | Universidade Federal Do Paraná | Extratos e frações de musa paradisiaca l. e seus usos |
Non-Patent Citations (2)
Title |
---|
Biochemical evaluation of hypoglycemic activity of Musa paradisiacal flowers in STZ induced experimental diabetes in rats;Shanmuga Sundaram C. et al;《Asian J. Research Chem》;20110530;第4卷(第5期);第827-831页 * |
香蕉花提取物抑制α-葡萄糖苷酶活性成分研究;王会;《中国优秀硕士学位论文全文数据库 农业科技辑》;20140215;第D048-257页 * |
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