CN107821021B

CN107821021B - interplanting method of Cassia alata and white clover

Info

Publication number: CN107821021B
Application number: CN201711134739.1A
Authority: CN
Inventors: 易显凤; 姚娜; 丘金花; 庞天德; 杨启晟; 邓素媛; 曾繁泉
Original assignee: Guangxi Zhuang Autonomous Region Institute of Animal Husbandry
Current assignee: Guangxi Zhuang Autonomous Region Institute of Animal Husbandry
Priority date: 2017-11-16
Filing date: 2017-11-16
Publication date: 2019-12-13
Anticipated expiration: 2037-11-16
Also published as: CN107821021A

Abstract

The invention relates to the technical field of planting, in particular to an interplanting method of cassia alata and white clover, which adopts the white clover and the cassia alata to interplant, and utilizes the super strong arsenic-rich capability of the white clover to achieve the purpose of improving the arsenic content in soil; simultaneously this application still utilizes the superstrong nitrogen fixation ability of the cassia tora of wing pod and reaches and provide sufficient nitrogen element for soil, guarantees that white clover grows and carries out the intergrowth with the cassia tora of wing pod, and this application carries out the preliminary treatment to white clover seed and the cassia seed of wing pod, can improve the germination percentage of the cassia tora of wing pod and white clover greatly, effectively promotes the growth of the cassia tora of wing pod and white clover, reaches the intergrowth of the cassia tora of wing pod and white clover, thereby reach and accelerate the soil fertility of supply, further improve the purpose of arsenic content in the soil.

Description

interplanting method of Cassia alata and white clover

[ technical field ] A method for producing a semiconductor device

The invention relates to the technical field of planting, in particular to an interplanting method of cassia wingnut and white clover.

[ background of the invention ]

In recent years, as industrial activities become more frequent and the soil becomes more polluted, arsenic accumulates in the soil and thus enters crop tissues. Arsenic and arsenide generally enter the body through water, atmosphere, and food, causing harm. The toxicity of the element arsenic is extremely low, the arsenides have toxicity, and the trivalent arsenic compound has stronger toxicity than other arsenic compounds; while white clover (Trifolium repens L) also known as white clover, white flower clover, white clover, lotus see-Shao, etc. are perennial herbs; because the growth cycle is short, the weeds are escaped to the field of dry crops sometimes, the harm is not serious, and the weeds are harmful to vegetables and young forests in local areas; however, the forage grass has wide adaptability and strong heat and cold resistance, can grow vigorously in acid soil and sandy soil, has certain ornamental value, is one of the main cultivated pastures in all countries in the world, is mainly used for grassland construction in China, and has good ecological and economic values; the white clover has the function of enriching arsenic, can effectively improve the phenomenon of overhigh content of arsenic in soil, but is difficult to coexist with other organisms and is easy to cause soil fertilizer shortage due to extremely strong growth capability of the white clover.

Cassia alata (academia alata) is a species of the genus Cassia of the family Leguminosae. Distributed in the southern region of Guangdong and Yunnan provinces. The natural plants grow on sparse forests or arid hillsides, and are widely distributed in tropical regions of America; has strong nitrogen fixation capacity, and if the cassia alata and the white clover can be planted together, the problems that the white clover needs fertilizer and the arsenic content in soil is too high can be solved.

Therefore, a method for interplanting the cassia alata and the white clover is needed, and the cassia alata and the white clover can be symbiotic, so that the purposes of accelerating soil fertility supplementation and further improving the arsenic content in soil are achieved.

[ summary of the invention ]

In view of the above, there is a need for a method for interplanting cassia alata and white clover, which can achieve the symbiosis of cassia alata and white clover, thereby achieving the purposes of accelerating soil fertility supplementation and further improving the arsenic content in soil.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

a method for interplanting Cassia alata and white clover comprises the following steps:

(1) Land block arrangement: turning the pre-ground and then mixing the pre-ground with the ground according to the proportion of 1kg/m²-2kg/m²The application amount of (2) is 500g/m of the disinfectant powder²-800g/m²Watering the pre-planted land with the watering amount, covering the pre-planted land with a mulching film, uncovering the mulching film after covering for 20-30 days, and applying organic fertilizer I to the pre-planted land, wherein the application amount of the organic fertilizer I is 1kg/m²-2kg/m²After fertilizing, curing for 15d-20d to obtain a planting land block;

(2) pretreatment of Cassia alata seeds: selecting healthy and plump Cassia alata seeds, mixing the Cassia alata seeds with a disinfectant according to a mass ratio of 1:2-3, soaking for 5-10 min, washing with distilled water, soaking the Cassia alata seeds and a seed soaking liquid according to a mass ratio of 1:3-4 for 24-26 h at a constant temperature of 25-30 ℃, filtering after soaking, draining the seeds, uniformly placing the seeds in a culture cup, and culturing under the conditions that the temperature is 25-30 ℃, the relative humidity is 10-15%, and the illumination intensity is 800Lx-1000Lx until the Cassia alata seedlings with the height of 5-7 cm grow out; the washing mode of the distilled water is as follows: filtering Cassia alata seeds from a disinfectant, taking out, mixing the Cassia alata seeds and distilled water according to the mass ratio of 1:2, stirring for 1min, removing the distilled water, mixing the Cassia alata seeds and the distilled water according to the mass ratio of 1:2, stirring, and repeatedly washing for 5-7 times;

(3) Planting Cassia alata: performing hole sowing on the Cassia alata seedlings in the step (2) on the planting land blocks in the step (1) according to the plant-row spacing of 30-40 cm multiplied by 50-60 cm, and watering the planting land blocks irregularly to keep the relative humidity of the planting land blocks at 20% -30%;

(4) pretreatment of white clover seeds: selecting healthy and plump white clover seeds, mixing the white clover seeds with a germination accelerating solution according to a mass ratio of 1:2-3, soaking for 20-30 min, filtering, then embedding the white clover seeds in sandy soil, taking out the seeds after 12-14 h, washing the white clover seeds with distilled water for 5-7 min, draining the seeds, uniformly placing the drained seeds in a culture cup II, and culturing under the conditions that the temperature is 28-32 ℃, the relative humidity is 20-25% and the illumination intensity is 1000Lx-1500Lx until white clover seedlings with the height of 5-8 cm grow out;

(5) interplanting white clover: planting the cassia tora alata seedlings pretreated in the step (4) between two adjacent rows of cassia tora alata 20-30 days after the cassia tora alata is planted in the step (3), and planting the seedlings according to the weight of 300g/m²-400g/m²The insect repellant is sprayed into the whole planting land, and the insect repellant is sprayed once again according to the same spraying amount after the interval of 20-30 days.

Further, the sterilizing powder in the step (1) comprises the following components in parts by weight: 15-25 parts of calcium oxide, 8-13 parts of eucalyptus extract, 9-15 parts of sweet wormwood extract, 7-13 parts of chrysanthemum root bark extract and 5-11 parts of chitosan.

Further, the organic fertilizer I in the step (1) comprises the following components in parts by weight: 5-10 parts of vermiculite, 15-25 parts of cassava dregs, 13-23 parts of mulberry leaf enzyme, 14-26 parts of soybean hulls and 23-35 parts of peanut bran.

Further, the disinfectant in the step (2) comprises the following components in percentage by weight: 0.1% -0.3% of HgCl₂1 to 3 percent of aloe extract, 2 to 5 percent of willow bark extract and the balance of water.

Further, the seed soaking liquid in the step (2) comprises the following components in percentage by weight: 1 to 3 percent of potassium permanganate, 1 to 3 percent of gibberellin, 1 to 3 percent of sodium chloride, 1 to 3 percent of okra extract and the balance of water.

Further, a culture solution is also filled in the culture cup in the step (2), and the culture solution comprises the following components in percentage by weight: 5 to 7 percent of cassava residue soak solution I, 2 to 5 percent of trehalose, 1 to 3 percent of spirulina extract, 1 to 3 percent of dragon fruit stem extract and the balance of water.

further, the germination accelerating liquid in the step (4) comprises the following components in percentage by weight: 8-10% of water fertilizer, 1-3% of indoleacetic acid, 1-3% of camellia oleifera dreg soaking solution, 1-3% of trehalose and the balance of water.

Further, a white clover culture solution is also filled in the culture cup II in the step (4), and the white clover culture solution comprises the following components in percentage by weight: 8-10% of fermentation bed waste padding soaking liquid, 2-4% of chitosan, 1-3% of spirulina extract, 1-3% of pawpaw extract and the balance of water.

Further, the insect repellent in the step (5) comprises the following components in percentage by weight: 2 to 5 percent of orange peel extract, 2 to 5 percent of eucalyptus extract, 2 to 5 percent of willow bark extract, 1 to 3 percent of lemon extract, 1 to 3 percent of sodium chloride and the balance of water.

The gibberellin used herein is (GA 3).

the invention has the following beneficial effects:

1. the invention can realize the interplanting and symbiosis of the white clover and the cassia alata, so as to provide enough nitrogen element for soil by utilizing the super strong nitrogen fixation capability of the cassia alata, ensure the growth of the white clover and the cassia alata, and achieve the aim of improving the arsenic content in the soil by utilizing the super strong arsenic-rich capability of the white clover; different from conventional planting, the method is characterized in that the planting land is disinfected by using disinfection powder in advance before planting, harmful pathogenic bacteria which are not suitable for growth of the trifolium repens in soil can be effectively killed, the disinfection powder is composed of calcium oxide, a eucalyptus extract, a sweet wormwood extract, a chrysanthemum root bark extract and chitosan, except the calcium oxide which is an inorganic substance and organic substance extracts, the formula has an active ingredient which can kill pathogenic microorganisms to kill viruses, and the active ingredient can be decomposed into organic substances through the effects of land turning, piling, retting and the like at the later stage, so that the environment cannot be polluted; this application is according to the growth characteristic of wing pod cassia tora, organic fertilizer I is applyed at the planting process in advance, organic fertilizer I is by the vermiculite, the cassava sediment, the mulberry leaf ferment, soybean shell and peanut bran are constituteed, wherein, contain abundant probiotic in addition to abundant organic matter among the cassava sediment, the mulberry leaf ferment, macromolecular substance in the organic fertilizer I can effectively be decomposed, especially vegetable protein, can turn into micromolecular substance with the organic matter, more do benefit to the plant and absorb, contain abundant protein in soybean shell and the peanut bran, be the main source of organic fertilizer nitrogen source, the vermiculite improves the effect of organic fertilizer porosity in organic fertilizer fermentation process, make organic fertilizer I porosity increase, promote the decomposition of microorganism to organic fertilizer I, improve the soil property before being fit for planting.

2. The method also comprises the steps of pretreating the seeds of the cassia wingpod, and disinfecting the seeds of the cassia wingpod by using a disinfectant in the pretreatment; the disinfectant consists of HgCl₂The disinfection solution contains Hg, and also contains abundant barbaloin, aloe polysaccharide, salicin and tannin components, which can play a broad-spectrum sterilization role and effectively kill harmful microorganisms in the seeds of the Cassia alata; the invention also uses seed soaking liquid to pretreat the cassia seed, the seed soaking liquid is composed of potassium permanganate, gibberellin, sodium chloride and okra extract; wherein potassium permanganate, gibberellin, okra polysaccharide and flavonoids can effectively improve the conversion rate of organic matters in the cassia wingnut seeds and can effectively improve the conversion rate of the organic matters in the cassia wingnut seedsGermination rate of Cassia alata seeds; the cassia wingpod culture solution is prepared from cassava residue soaking solution I, trehalose, a spirulina extract, vitamin C in a dragon fruit stem extract and dragon fruit polysaccharide, and can obviously improve the growth capacity of cassia wingpod seeds.

3. The method also comprises the steps of pretreating the white clover, wherein the white clover is pretreated by using a germination accelerating liquid in the pretreatment, and the germination accelerating liquid consists of a water fertilizer, indoleacetic acid, a tea-oil tree residue soaking liquid and trehalose; wherein the water fertilizer can provide fertility for the white clover, the indoleacetic acid can promote the transformation of the white clover seeds, and the tea saponin in the camellia oleifera residue soaking solution can improve the antibacterial capacity of the white clover; the application also uses the white clover culture solution to culture the white clover, and the white clover culture solution can promote the growth of the white clover by soaking chitosan, linolenic acid substances, vitamins, pawpaw extract saponin and flavonoid substances in the waste padding of a fermentation bed; the insect repellent consists of an orange peel extract, a eucalyptus extract, a willow bark extract and sodium chloride of a lemon extract, wherein limonin in the orange peel extract, a terpene component in the eucalyptus extract, a tannin component, a phloroglucinol component, a glucoside component, salicin in the willow bark extract, a tannin component, limonin in the lemon extract and tannin have a broad-spectrum sterilization effect and can effectively repel most insect pests.

[ detailed description ] embodiments

all of the features disclosed in this specification, or all of the steps in any method or process so disclosed, may be combined in any combination, except combinations of features and/or steps that are mutually exclusive.

Any feature disclosed in this specification (including any accompanying claims, abstract) is merely an example of a generic series of equivalent or similar features, unless explicitly described as such.

example 1:

(1) Land block arrangement: turning the pre-ground and then mixing the pre-ground with the ground according to the proportion of 1kg/m²is applied in a predetermined amountSpreading antivirus powder at 500g/m²Watering the pre-planted land with the watering amount, covering the pre-planted land with a mulching film, uncovering the mulching film after covering for 20 days, and applying organic fertilizer I to the pre-planted land, wherein the application amount of the organic fertilizer I is 1kg/m²after fertilization, curing for 15d to obtain a planting land block;

(2) Pretreatment of Cassia alata seeds: selecting healthy and plump Cassia alata seeds, mixing the Cassia alata seeds with a disinfectant according to a mass ratio of 1:2, soaking for 5min, washing with distilled water, soaking the Cassia alata seeds and a seed soaking liquid according to a mass ratio of 1:3 at a constant temperature of 25 ℃ for 24h, filtering after soaking, draining the seeds, uniformly placing the seeds in a culture cup, and culturing under the conditions of a temperature of 25 ℃, a relative humidity of 10% and a light intensity of 800Lx until Cassia alata seedlings with a height of 5cm grow out; the washing mode of the distilled water is as follows: filtering Cassia alata seeds from a disinfectant, taking out, mixing the Cassia alata seeds and distilled water according to the mass ratio of 1:2, stirring for 1min, removing the distilled water, mixing the Cassia alata seeds and the distilled water according to the mass ratio of 1:2, stirring, and repeatedly washing for 5-7 times;

(3) planting Cassia alata: performing hole sowing on the Cassia alata seedlings in the step (2) on the planting land in the step (1) according to the plant-row spacing of 30cm multiplied by 50cm, and watering the planting land irregularly to keep the relative humidity of the planting land at 20%;

(4) Pretreatment of white clover seeds: selecting healthy and plump white clover seeds, mixing the white clover seeds with a germination accelerating solution according to a mass ratio of 1:2, soaking for 20min, filtering, then embedding the white clover seeds in sandy soil, taking out the seeds after 12h, washing the white clover seeds with distilled water for 5min, draining the seeds, uniformly placing the seeds in a culture cup II, and culturing under the conditions that the temperature is 28 ℃, the relative humidity is 20% and the illumination intensity is 1000Lx until white clover seedlings with the height of 5cm grow out;

(5) Interplanting white clover: planting the cassia tora alata seedlings pretreated in the step (4) between two adjacent rows of cassia tora alata 20 days after planting the cassia tora alata in the step (3), and planting the seedlings according to the weight of 300g/m²the insect repellent is sprayed into the whole planting plots at intervals of 20 days, and the insects are sprayed again according to the same spraying amount.

wherein, the sterilizing powder in the step (1) comprises the following components in parts by weight: 15 parts of calcium oxide, 8 parts of eucalyptus extract, 9 parts of sweet wormwood extract, 7 parts of chrysanthemum root bark extract and 5 parts of chitosan.

The method for extracting the eucalyptus extract from the sterilizing powder comprises the following steps: mixing eucalyptus leaves with the water content of 5% and bark according to the mass ratio of 1:2, crushing, screening by a 100-mesh screen, dividing the powder into two parts, putting one part into a butanol solution with the mass ratio of 3 times and the volume percentage of 20% of butanol, performing reflux extraction for 22 hours, filtering, taking the solvent, performing reduced pressure distillation, and drying until the water content is 3% to obtain a eucalyptus extract I; and putting the other part into a petroleum ether solution with the mass part being 4 times and the volume percentage of the petroleum ether being 10%, performing reflux extraction for 24 hours, filtering, taking the solvent, performing reduced pressure distillation, and drying until the water content is 3% to obtain a eucalyptus extract II. In the eucalyptus extract, the content of terpenoid components is 45.36mg/g, the content of flavonoid components is 187.14mg/g, the content of tannin components is 25.87mg/g, the content of phloroglucinol components is 121.32mg/g, and the content of glucoside components is 109.76 mg/g.

② the extraction method of the sweet wormwood extract in the sterilizing powder is as follows: mashing fresh sweet wormwood, adding 3 times of ethanol solution with the volume percentage of 80% ethanol, uniformly mixing, putting the mixture into an ultrasonic extractor for ultrasonic extraction, wherein the ultrasonic extraction power is 20kHz and 90W ultrasonic waves, the extraction temperature is 50 ℃, after single action for 20min, stopping 1min, continuing to perform single action for 20min, repeating the single action for 5 times, filtering, taking filtrate, performing rotary evaporation concentration and drying until the water content is 4%, and obtaining sweet wormwood extract, wherein the artemisinin component in the extract is 87.88%.

③ the extraction method of the chrysanthemum root-bark extract in the sterilizing powder is as follows: drying chrysanthemum root bark until the water content is 4%, then mixing chrysanthemum root and chrysanthemum bark according to the mass ratio of 1:2, crushing, screening by a 40-mesh screen, dividing the powder into two parts, putting one part into a butanol solution with the mass ratio of 3 times that of the chrysanthemum root and the chrysanthemum bark and the butanol of 25% by volume, performing reflux extraction for 30 hours, filtering, taking the solvent, performing reduced pressure distillation, and drying until the water content is 3% to obtain a chrysanthemum root bark extract I; and putting the other part into an acetone solution with the mass part being 6 times that of the chrysanthemum root bark extract II and the volume percentage of acetone being 10%, performing reflux extraction for 32 hours, filtering, taking the solvent, performing reduced pressure distillation, and drying until the water content is 3% to obtain the chrysanthemum root bark extract II. In the chrysanthemum root-bark extract, the content of the marigold-3-O-glucoside is 361.24mg/g, and the content of the flavonoid component is 518.03 mg/g.

The preparation method of the antivirus powder comprises the following steps: respectively screening the calcium oxide, the eucalyptus extract, the sweet wormwood extract, the chrysanthemum root bark extract and the chitosan through 200-mesh screens, weighing the components in parts by weight, and mixing the components to obtain the chrysanthemum health-care tea.

Wherein the organic fertilizer I in the step (1) comprises the following components in parts by weight: 5 parts of vermiculite, 15 parts of manioc waste, 13 parts of mulberry leaf enzyme, 14 parts of soybean hulls and 23 parts of peanut bran.

The cassava residues in the organic fertilizer I are derived from the following raw materials: the organic matter content of the waste residue obtained after the cassava alcohol fermentation can reach 90 percent, wherein the COD content is 200 mg/L.

② the mulberry leaf enzyme in the organic fertilizer I is: putting fresh mulberry leaves into a constant-temperature fermentation chamber with the temperature of 25 ℃ and the relative humidity of 15% for stack retting, and spraying sucrose water into the mulberry leaves according to the addition of 20mg/g during stack retting, wherein the concentration of the sucrose water is 3 mol/L; and (3) retting for 10 days, adding dry yeast into the mulberry leaves according to the addition of 5mg/g, uniformly stirring, and sealing and fermenting the fermentation chamber for 40 days to obtain the mulberry leaf enzyme.

The production method of the organic fertilizer I comprises the following steps: weighing the raw materials according to the weight parts, uniformly mixing, and then putting the mixture into an anaerobic fermentation chamber with the temperature of 28 ℃ and the relative humidity of 15% for fermentation for 10 days.

wherein, the disinfectant in the step (2) comprises the following components in percentage by weight: 0.1% HgCl₂1% of aloe extract, 2% of willow bark extract and the balance of water.

The extraction method of the aloe extract in the disinfectant comprises the following steps: crushing fresh aloe with skin, adding 70% ethanol solution according to the mass ratio of aloe to ethanol of 1:4, standing at-3 deg.C for 24h, ultrasonic extracting in an ultrasonic extractor at 50 deg.C for 30min at 500w, reflux-extracting in a reflux extractor at 100 deg.C for 6h, filtering, concentrating the filtrate by rotary evaporation, and drying until the water content is 3% to obtain aloe extract. The content of barbaloin in the extract is 132.45 mg/g; the aloe polysaccharide content is 245.98 mg/g.

② the method for extracting willow bark extract in the disinfection solution comprises: drying willow bark until the water content of the willow bark is 5%, then crushing the willow bark, sieving the willow bark with a 100-mesh sieve to obtain willow bark powder, mixing the willow bark powder and an ethanol solution according to the mass ratio of 1:6, and standing for 24 hours, wherein the volume percentage of the ethanol solution is 95%; standing, placing the mixture into a reflux extractor for reflux extraction for 12h, filtering, distilling the solvent under reduced pressure, and drying until the water content is 3% to obtain the chrysanthemum root bark extract II. The extract contains salicin 541.26mg/g and tannin 42.03 mg/g.

The preparation method of the disinfectant comprises the following steps: weighing HgCl according to the weight percentage₂mixing with Aloe extract, willow bark extract and water, slowly heating to 50 deg.C, stirring at constant temperature for 2min, and cooling to room temperature to obtain disinfectant.

Wherein, the seed soaking liquid in the step (2) comprises the following components in percentage by weight: 1% of potassium permanganate, 1% of gibberellin, 1% of sodium chloride, 1% of okra extract and the balance of water.

The method for extracting the okra extract in the seed soaking liquid comprises the following steps: crushing fresh okra with skin, adding 95% ethanol solution according to the weight ratio of okra to ethanol of 1:5, standing at-3 ℃ for 12h, placing into an ultrasonic extractor for ultrasonic extraction with the ultrasonic extraction power of 800w, the extraction temperature of 60 ℃ and the extraction time of 50min, placing into a reflux extractor for reflux extraction with the extraction temperature of 95 ℃ and the extraction time of 8h, filtering, taking the filtrate, performing rotary evaporation concentration and drying until the water content is 3%, and thus obtaining the okra extract. The content of okra polysaccharide in the extract is 368.32 mg/g; the content of flavonoids is 465.54 mg/g.

The configuration method of the seed soaking liquid comprises the following steps: weighing potassium permanganate, gibberellin, sodium chloride, okra extract and water according to the weight percentage, mixing, slowly heating to 60 ℃, stirring at constant temperature for 2min, and cooling to room temperature to obtain seed soaking liquid.

The culture cup in the step (2) is also filled with a culture solution, and the culture solution comprises the following components in percentage by weight: 5% of cassava residue soak solution I, 2% of trehalose, 1% of spirulina extract, 1% of dragon fruit stem extract and the balance of water.

The method for soaking the cassava residue soaking solution I in the culture solution comprises the following steps: drying waste residues generated in alcohol production by cassava fermentation until the water content is 10%, mixing the waste residues and distilled water according to the mass ratio of 2:1, heating to 60 ℃, soaking at constant temperature for 48h, and filtering to obtain a cassava residue soaking solution I. The organic matter content in the soaking solution can reach 534.32 mg/g.

② the extraction method of spirulina extract in the culture solution is: mixing spirulina and ethanol at a mass ratio of 1:8 with 95% ethanol, standing at-3 deg.C for 8h, ultrasonic extracting at 800w and 60 deg.C for 60min, filtering, concentrating the filtrate by rotary evaporation, and drying until the water content is 3% to obtain spirulina extract. The linoleic acid content in the extract is 416.28 mg/g; the vitamin content was 95.54 mg/g.

③ the extraction method of the pitaya stem extract in the culture solution comprises the following steps: peeling and grinding the dragon fruit stems, adding 70% ethanol solution according to the mass ratio of the dragon fruit stems to the ethanol of 1:4, standing for 18h under a freezing condition at the temperature of-3 ℃, performing reflux extraction at a constant temperature of 100 ℃ for 6h, filtering, taking filtrate, performing rotary evaporation concentration and drying until the water content is 3%, and thus obtaining the dragon fruit stem extract. The content of vitamin C in the extract is 154.25 mg/g; the content of dragon fruit polysaccharide is 365.25 mg/g.

The preparation method of the culture solution comprises the following steps: weighing the cassava residue soaking solution I, trehalose, a spirulina extract, a dragon fruit stem extract and water according to the weight percentage, mixing, slowly heating to 55 ℃, stirring at constant temperature for 2min, and cooling to room temperature to obtain a culture solution.

Wherein, the germination accelerating liquid in the step (4) comprises the following components in percentage by weight: 8% of water fertilizer, 1% of indoleacetic acid, 1% of camellia oleifera residue soaking solution, 1% of trehalose and the balance of water.

The water fertilizer in the germination accelerating liquid is prepared by the following method: diluting animal urine with water according to a ratio of 1:4 to obtain diluted animal urine; soaking the cassava residue waste produced by alcohol with water for 12 hours according to the ratio of 3:1, and then filtering to remove filter residues to obtain cassava residue soaking liquid; soaking waste fermentation bed padding materials with water for 12 hours at a ratio of 4:1, and filtering to remove filter residues to obtain fermentation bed padding material soaking liquid; and mixing the diluted animal urine, the cassava residue soaking solution and the fermentation bed padding soaking solution according to the mass ratio of 2:1:1 to obtain the water fertilizer.

② the tea-leaf soaking liquid in the germination accelerating liquid is prepared by the following method: mixing oil tea residues with oil content of 5% after the tea seed oil is squeezed and water according to the mass ratio of 4:1, soaking for 12 hours at the constant temperature of 80 ℃, filtering to remove filter residues, and cooling to room temperature to obtain an oil tea residue soaking solution, wherein the content of tea saponin in the soaking solution is 123.87 mg/g.

the preparation method of the germination accelerating liquid comprises the following steps: weighing the water fertilizer, the indoleacetic acid, the camellia oleifera dreg soaking solution, the trehalose and the water according to the weight percentage, mixing, slowly heating to 65 ℃, stirring at constant temperature for 2min, and cooling to room temperature to obtain the germination accelerating liquid.

Wherein, the white clover culture solution is also filled in the culture cup II in the step (4), and consists of the following components in percentage by weight: 8% of fermentation bed waste padding soaking liquid, 2% of chitosan, 1% of spirulina extract, 1% of pawpaw extract and the balance of water.

The soaking solution of the waste padding of the fermentation bed in the white clover culture solution is prepared by the following method: mixing and heating the waste padding of the fermentation bed and water according to the mass ratio of 4:1 to 60 ℃, soaking at constant temperature for 12 hours, filtering to remove filter residues, and cooling the filtrate to room temperature to obtain the waste padding soaking solution of the fermentation bed.

② the spirulina extract in the white clover culture solution is prepared by the following method: mixing spirulina and ethanol at a mass ratio of 1:8 with 95% ethanol, standing at-3 deg.C for 8h, ultrasonic extracting at 800w and 60 deg.C for 60min, filtering, concentrating the filtrate by rotary evaporation, and drying until the water content is 3% to obtain spirulina extract. The linoleic acid content in the extract is 416.28 mg/g; the vitamin content was 95.54 mg/g.

③ the pawpaw extract in the white clover culture solution is prepared by the following method: mixing the papaya peel and the papaya flesh according to a mass ratio of 1:4, then mixing the mixture with 95% ethanol according to a mass ratio of 1:1, grinding, then putting into water at a temperature of 60 ℃ for constant-temperature water bath for 12 hours, then filtering to remove filter residues, carrying out rotary evaporation concentration on the filtrate, and drying until the water content is 3% to obtain a papaya extract; the saponin content in the extract is 109.76 mg/g; the content of flavonoids is 421.42 mg/g.

The preparation method of the white clover culture solution comprises the following steps: weighing the fermentation bed waste padding soaking solution, chitosan, spirulina extract, pawpaw extract and water according to the weight percentage, mixing, slowly heating to 50 ℃, stirring at constant temperature for 2min, and cooling to room temperature to obtain the white clover culture solution.

wherein, the insect repellant prepared in the step (5) comprises the following components in percentage by weight: 2% of orange peel extract, 2% of eucalyptus extract, 2% of willow bark extract, 1% of lemon extract, 1% of sodium chloride and the balance of water.

The orange peel extract in the insect repellent is prepared by the following method: crushing orange peel with the water content of 5%, mixing the orange peel with butanol with the volume percentage of 20% according to the mass ratio of 1:3, placing the mixture in a freezer with the temperature of-2 ℃ for standing for 10 hours, placing the mixture in a reflux extractor with the temperature of 100 ℃ for reflux extraction for 4 hours, filtering to remove filter residues, carrying out rotary evaporation concentration on the filtrate, and drying until the water content is 3% to obtain an orange peel extract; the content of vitamin C in the extract is 98.87 mg/g; the content of limonin is 187.09 mg/g.

② the eucalyptus extract in the insect repellant is prepared by the following method: mixing eucalyptus leaves with the water content of 5% and bark according to the mass ratio of 1:2, crushing, screening by a 100-mesh screen, dividing the powder into two parts, putting one part into a butanol solution with the mass ratio of 3 times and the volume percentage of 20% of butanol, performing reflux extraction for 22 hours, filtering, taking the solvent, performing reduced pressure distillation, and drying until the water content is 3% to obtain a eucalyptus extract I; and putting the other part into a petroleum ether solution with the mass part being 4 times and the volume percentage of the petroleum ether being 10%, performing reflux extraction for 24 hours, filtering, taking the solvent, performing reduced pressure distillation, and drying until the water content is 3% to obtain a eucalyptus extract II. In the eucalyptus extract, the content of terpenoid components is 45.36mg/g, the content of flavonoid components is 187.14mg/g, the content of tannin components is 25.87mg/g, the content of phloroglucinol components is 121.32mg/g, and the content of glucoside components is 109.76 mg/g.

③ the willow bark extract in the insect repellant is prepared by the following method: drying willow bark until the water content of the willow bark is 5%, then crushing the willow bark, sieving the willow bark with a 100-mesh sieve to obtain willow bark powder, mixing the willow bark powder and an ethanol solution according to the mass ratio of 1:6, and standing for 24 hours, wherein the volume percentage of the ethanol solution is 95%; standing, placing the mixture into a reflux extractor for reflux extraction for 12h, filtering, distilling the solvent under reduced pressure, and drying until the water content is 3% to obtain the chrysanthemum root bark extract II. The extract contains salicin 541.26mg/g and tannin 42.03 mg/g.

fourthly, the lemon extract in the insect repellant is prepared by the following method: mashing the whole lemon, mixing the mashed lemon with an ethanol solution with the volume percentage of 95% according to the mass ratio of 1:3, then placing the mixture into a freezer with the temperature of-5 ℃ for standing for 10h, then placing the mixture into an ultrasonic extractor for ultrasonic extraction, wherein the extraction temperature is 60 ℃, the extraction time is 60min, the extraction power is 800w, then placing the mixture into a reflux extractor with the temperature of 100 ℃ for reflux extraction for 8h, filtering to remove filter residues, carrying out rotary evaporation concentration on the filtrate, and drying until the water content is 3% to obtain a lemon extract; the content of vitamin C in the extract is 128.43 mg/g; the content of limonin is 216.21 mg/g; the tannin content was 32.42 mg/g.

The preparation method of the insect repellant comprises the following steps: weighing the orange peel extract, the eucalyptus extract, the willow bark extract, the lemon extract, the sodium chloride and water according to the weight percentage, mixing, slowly heating to 70 ℃, stirring at constant temperature for 2min, and cooling to room temperature to obtain the insect repellent.

Example 2:

(1) Land block arrangement: turning the pre-ground and then according to the proportion of 2kg/m²The application amount of (2) is that the antivirus powder is thrown into the pre-ground land according to the proportion of 800g/m²watering the pre-planted land with the watering amount, covering the pre-planted land with a mulching film, uncovering the mulching film after covering for 30 days, and applying an organic fertilizer I to the pre-planted land, wherein the application amount of the organic fertilizer I is 2kg/m²after fertilization, curing for 20d to obtain a planting land block;

(2) Pretreatment of Cassia alata seeds: selecting healthy and plump Cassia alata seeds, mixing the Cassia alata seeds with a disinfectant according to a mass ratio of 1:3, soaking for 10min, washing with distilled water, soaking the Cassia alata seeds and a seed soaking liquid according to a mass ratio of 1:4 at a constant temperature of 30 ℃ for 26h, filtering after soaking, draining the seeds, uniformly placing the seeds in a culture cup, and culturing under the conditions of a temperature of 30 ℃, a relative humidity of 15% and a light intensity of 1000Lx until Cassia alata seedlings with a height of 7cm grow out; the washing mode of the distilled water is as follows: filtering Cassia alata seeds from a disinfectant, taking out, mixing the Cassia alata seeds and distilled water according to the mass ratio of 1:2, stirring for 1min, removing the distilled water, mixing the Cassia alata seeds and the distilled water according to the mass ratio of 1:2, stirring, and repeatedly washing for 7 times;

(3) Planting Cassia alata: performing hole sowing on the Cassia alata seedlings in the step (2) on the planting land in the step (1) according to the plant-row spacing of 40cm multiplied by 60cm, and watering the planting land irregularly to keep the relative humidity of the planting land at 30%;

(4) Pretreatment of white clover seeds: selecting healthy and plump white clover seeds, mixing the white clover seeds with a germination accelerating solution according to a mass ratio of 1:3, soaking for 30min, filtering, then embedding the white clover seeds in sandy soil, taking out the seeds after 14h, washing the white clover seeds with distilled water for 7min, draining the seeds, uniformly placing the seeds in a culture cup II, and culturing under the conditions that the temperature is 32 ℃, the relative humidity is 25% and the illumination intensity is 1500Lx until white clover seedlings with the height of 8cm grow out;

(5) Interplanting white clover: planting the cassia tora alata seedlings pretreated in the step (4) between two adjacent rows of cassia tora alata after planting the cassia tora alata in the step (3) for 30 days, and planting the seedlings according to the ratio of 400g/m²The insect repellent is sprayed into the whole planting plots at the spraying amount, and the insect repellent is sprayed once again according to the same spraying amount after the interval of 30 d.

Wherein, the sterilizing powder in the step (1) comprises the following components in parts by weight: 25 parts of calcium oxide, 13 parts of eucalyptus extract, 15 parts of sweet wormwood extract, 13 parts of chrysanthemum root bark extract and 11 parts of chitosan.

(the extraction method of the antivirus powder eucalyptus extract, the extraction method of the artemisia apiacea extract, the extraction method of the chrysanthemum root bark extract and the preparation method of the antivirus powder are completely the same as the embodiment 1.)

wherein the organic fertilizer I in the step (1) comprises the following components in parts by weight: 10 parts of vermiculite, 25 parts of manioc waste, 23 parts of mulberry leaf enzyme, 26 parts of soybean hulls and 35 parts of peanut bran.

(the source of the cassava residue in the organic fertilizer I, the preparation method of the mulberry leaf enzyme and the production method of the organic fertilizer I are completely the same as those in the example 1.)

Wherein the disinfectant in the step (2) is prepared from the following components in percentage by weightThe components by weight percentage are as follows: 0.3% HgCl₂3% of aloe extract, 5% of willow bark extract and the balance of water.

(the method for extracting aloe extract, the method for extracting willow bark extract and the method for preparing the disinfectant are completely the same as in example 1.)

Wherein, the seed soaking liquid in the step (2) comprises the following components in percentage by weight: 3 percent of potassium permanganate, 3 percent of gibberellin, 3 percent of sodium chloride, 3 percent of okra extract and the balance of water.

(the extraction method of the okra extract in the seed soaking solution and the preparation method of the seed soaking solution are completely the same as those in example 1.)

The culture cup in the step (2) is also filled with a culture solution, and the culture solution comprises the following components in percentage by weight: 7% of cassava residue soak solution I, 5% of trehalose, 3% of spirulina extract, 3% of dragon fruit stem extract and the balance of water.

(the method for soaking the cassava residue soak solution I in the culture solution, the method for extracting the spirulina extract, the method for extracting the dragon fruit stem extract and the preparation method of the culture solution are completely the same as those in the example 1.)

Wherein, the germination accelerating liquid in the step (4) comprises the following components in percentage by weight: 10% of water fertilizer, 3% of indoleacetic acid, 3% of camellia oleifera residue soaking solution, 3% of trehalose and the balance of water.

(the preparation method of the water and fertilizer in the germination accelerating liquid, the tea-oil tree residue soaking method and the preparation method of the germination accelerating liquid are completely the same as those in the example 1.)

Wherein, the culture cup II in the step (4) is also filled with a white clover culture solution, and the culture solution comprises the following components in percentage by weight: 10% of fermentation bed waste padding soaking liquid, 4% of chitosan, 3% of spirulina extract, 3% of pawpaw extract and the balance of water.

(the preparation method of the fermentation bed waste padding soaking solution in the white clover culture solution, the extraction method of the spirulina extract, the extraction method of the pawpaw extract and the preparation method of the culture solution are completely the same as the embodiment 1.)

Wherein, the insect repellant prepared in the step (5) comprises the following components in percentage by weight: 5% of orange peel extract, 5% of eucalyptus extract, 5% of willow bark extract, 3% of lemon extract, 3% of sodium chloride and the balance of water.

(the method of extracting orange peel extract, the method of extracting eucalyptus extract, the method of extracting willow bark extract, the method of extracting lemon extract, and the method of preparing insect repellent were completely the same as in example 1.)

Example 3:

(1) Land block arrangement: turning the pre-ground land, and then according to the proportion of 1.5kg/m²the application amount of (2) is that the antivirus powder is sprinkled into the pre-ground land according to 600g/m²Watering the pre-planted land with the watering amount, covering the pre-planted land with a mulching film, uncovering the mulching film after covering for 25 days, and applying organic fertilizer I to the pre-planted land, wherein the application amount of the organic fertilizer I is 1.5kg/m²after fertilization, curing for 17d to obtain a planting land parcel;

(2) pretreatment of Cassia alata seeds: selecting healthy and plump Cassia alata seeds, mixing the Cassia alata seeds with a disinfectant according to a mass ratio of 1:2.5, soaking for 7min, washing with distilled water, soaking the Cassia alata seeds and a seed soaking liquid according to a mass ratio of 1:3.5 at a constant temperature of 27 ℃ for 25h, filtering after soaking, draining the seeds, uniformly placing the seeds in a culture cup, and culturing under the conditions of a temperature of 27 ℃, a relative humidity of 12% and a light intensity of 900Lx until Cassia alata seedlings with a height of 6cm grow out; the washing mode of the distilled water is as follows: filtering Cassia alata seeds from a disinfectant, taking out, mixing the Cassia alata seeds and distilled water according to the mass ratio of 1:2, stirring for 1min, removing the distilled water, mixing the Cassia alata seeds and the distilled water according to the mass ratio of 1:2, stirring, and repeatedly washing for 5-7 times;

(3) planting Cassia alata: performing hole sowing on the Cassia alata seedlings in the step (2) on the planting land in the step (1) according to the plant-row spacing of 35cm multiplied by 55cm, and watering the planting land irregularly to keep the relative humidity of the planting land at 25%;

(4) pretreatment of white clover seeds: selecting healthy and plump white clover seeds, mixing the white clover seeds with a germination accelerating solution according to a mass ratio of 1:2.5, soaking for 25min, filtering, then embedding the white clover seeds in sandy soil, taking out the seeds after 13h, washing the white clover seeds with distilled water for 6min, draining the seeds, uniformly placing the seeds in a culture cup II, and culturing under the conditions that the temperature is 30 ℃, the relative humidity is 22% and the illumination intensity is 1200Lx until white clover seedlings with the height of 7cm grow out;

(5) Interplanting white clover: planting the white clover seedlings pretreated in the step (4) between two adjacent rows of cassia wingnuts after planting the cassia wingnuts in the step (3) for 25 days, and planting the white clover seedlings according to 350g/m²The insect repellent was sprayed to the entire planting plots at the same amount of spraying, and the same amount of spraying was applied again after an interval of 25 d.

Wherein, the sterilizing powder in the step (1) comprises the following components in parts by weight: 20 parts of calcium oxide, 10 parts of eucalyptus extract, 12 parts of sweet wormwood extract, 8 parts of chrysanthemum root bark extract and 6 parts of chitosan.

Wherein the organic fertilizer I in the step (1) comprises the following components in parts by weight: 7 parts of vermiculite, 20 parts of manioc waste, 18 parts of mulberry leaf enzyme, 20 parts of soybean hulls and 30 parts of peanut bran.

Wherein, the disinfectant in the step (2) comprises the following components in percentage by weight: 0.2% HgCl₂2% of aloe extract, 3% of willow bark extract and the balance of water.

wherein, the seed soaking liquid in the step (2) comprises the following components in percentage by weight: 2 percent of potassium permanganate, 2 percent of gibberellin, 2 percent of sodium chloride, 2 percent of okra extract and the balance of water.

The culture cup in the step (2) is also filled with a culture solution, and the culture solution comprises the following components in percentage by weight: 6% of cassava residue soak solution I, 4% of trehalose, 2% of spirulina extract, 2% of dragon fruit stem extract and the balance of water.

Wherein, the germination accelerating liquid in the step (4) comprises the following components in percentage by weight: 9% of water fertilizer, 2% of indoleacetic acid, 2% of camellia oleifera residue soaking solution, 2% of trehalose and the balance of water.

Wherein, the culture cup II in the step (4) is also filled with a white clover culture solution, and the culture solution comprises the following components in percentage by weight: 9% of fermentation bed waste padding soaking liquid, 3% of chitosan, 2% of spirulina extract, 2% of pawpaw extract and the balance of water.

Wherein, the insect repellant prepared in the step (5) comprises the following components in percentage by weight: 3% of orange peel extract, 3% of eucalyptus extract, 3% of willow bark extract, 2% of lemon extract, 2% of sodium chloride and the balance of water.

control group 1:

the control group was planted exactly as in example 1, but the plots were not pre-treated with a sanitizer, and the other methods were exactly the same as in example 1.

Namely: the method comprises the following steps:

(1) Land block arrangement: turning the pre-planted land, and applying organic fertilizer I in an amount of 1kg/m to the pre-planted land²after fertilization, curing for 15d to obtain a planting land block;

Control group 2:

The control group was planted exactly in the same way as in example 1, but the plots were not fertilized with organic fertilizer I, and the other methods were exactly the same as in example 1.

Namely: the method comprises the following steps:

(1) Land block arrangement: turning the pre-ground and then mixing the pre-ground with the ground according to the proportion of 1kg/m²The application amount of (2) is 500g/m of the disinfectant powder²Watering the pre-planted land with the watering amount, then covering the pre-planted land with a mulching film, and uncovering the mulching film after covering for 20 days to obtain a planting land block;

Control group 3:

This control was grown exactly as in example 1, but the cassia winged seed was not pretreated and the other procedure was exactly the same as in example 1.

Namely: the method comprises the following steps:

(1) Land block arrangement: turning the pre-ground and then mixing the pre-ground with the ground according to the proportion of 1kg/m²The application amount of (2) is 500g/m of the disinfectant powder²watering the pre-planted land with the watering amount, covering the pre-planted land with a mulching film, uncovering the mulching film after covering for 20 days, and applying organic fertilizer I to the pre-planted land, wherein the application amount of the organic fertilizer I is 1kg/m²after fertilization, curing for 15d to obtain a planting land block;

(2) Pretreatment of Cassia alata seeds: selecting healthy and plump Cassia alata seeds, and planting the Cassia alata seeds in sand until Cassia alata seedlings with the height of 5cm grow out;

Control group 4:

the control group was planted exactly in the same manner as in example 1, except that the white clover seeds were not pretreated, and the other methods were exactly the same as in example 1.

Namely: the method comprises the following steps:

(4) Pretreatment of white clover seeds: selecting healthy and plump white clover seeds, and directly sowing the white clover seeds in sandy soil until white clover seedlings with the height of 5cm grow out;

Control group 5:

The control group was planted exactly in the same manner as in example 1, but only cassia winglets were planted, and the other method was exactly the same as in example 1.

Namely: the method comprises the following steps:

(3) planting Cassia alata: and (3) performing hole sowing on the cassia wingpod seedlings in the step (2) on the planting land in the step (1) according to the plant-row spacing of 30cm multiplied by 50cm, and watering the planting land irregularly to keep the relative humidity of the planting land at 20%.

Control group 6:

the control group was planted in exactly the same manner as in example 1, but only white clover was planted, and the other methods were exactly the same as in example 1.

Namely: the method comprises the following steps:

(2) Pretreatment of white clover seeds: selecting healthy and plump white clover seeds, mixing the white clover seeds with a germination accelerating solution according to a mass ratio of 1:2, soaking for 20min, filtering, then embedding the white clover seeds in sandy soil, taking out the seeds after 12h, washing the white clover seeds with distilled water for 5min, draining the seeds, uniformly placing the seeds in a culture cup II, and culturing under the conditions that the temperature is 28 ℃, the relative humidity is 20% and the illumination intensity is 1000Lx until white clover seedlings with the height of 5cm grow out;

(3) Planting white clover: planting white clover at plant row spacing of 20cm × 20cm, and at a ratio of 300g/m²Spraying amount of the fertilizer to the whole planting land block to expel insectsthe agent is sprayed once more according to the same spraying amount after 20 days.

Test run 1:

Experiments are completed in an experimental base of the research institute, an experimental plot is artificially manufactured to have the arsenic (As) -rich environment arsenic content of 73.43mg/kg, planting is carried out according to the methods of examples 1-3 and comparison groups 1-6, after 1 year of planting, a surface impurity layer is uniformly scraped, and the nitrogen, phosphorus and arsenic (As) content and organic matter content of a soil layer with the thickness of 2cm, a soil layer with the thickness of 5cm, a soil layer with the thickness of 10cm and a soil layer with the thickness of 20cm are tested, and the contents are specifically shown in Table 1:

TABLE 1

As can be seen from the above table, the nitrogen, phosphorus and organic matter contents of the soil layer with thickness of 2cm, the soil layer with thickness of 5cm, the soil layer with thickness of 10cm and the soil layer with thickness of 20cm in the examples 1-3 are all higher than those of the control group 1-6, while the nitrogen, phosphorus and organic matter contents of the soil layer with thickness of 2cm, the soil layer with thickness of 5cm, the soil layer with thickness of 10cm and the soil layer with thickness of 20cm in the control group 1-6 are the lowest, which indicates that the interplanting method of the white clover and the cassia winged Cassia used in the present application has stronger nitrogen fixation capability than that of singly planting the white clover or the cassia winged Cassia and can also significantly improve the phosphorus and organic matter contents in the soil; while the arsenic (As) content of the soil layer with the thickness of 2cm, the soil layer with the thickness of 5cm, the soil layer with the thickness of 10cm and the soil layer with the thickness of 20cm in the examples 1-3 is obviously lower than that of the control group 1-6, the arsenic (As) content of the soil layer with the thickness of 2cm, the soil layer with the thickness of 5cm, the soil layer with the thickness of 10cm and the soil layer with the thickness of 20cm in the control group 5 and the control group 6 is the highest in the control group 1-6, which shows that the interplanting method of the white clover and the wing pod cassia used in the application has the stronger purpose of reducing the arsenic (As) content in the soil than the single planting of the white clover or the wing pod cassia tora.

test run 2:

experiments were completed in the experimental sites of the institute, planting was performed according to the methods of examples 1-3 and control groups 1-6, 3 seedlings were randomly selected for each treatment after 10 days of white clover planting, and the seedling heights of the cassia winged pod and white clover were measured with a ruler, respectively. The details are shown in Table 2:

TABLE 2

From the above table, it can be seen that: the seedlings of examples 1-3 are significantly higher than those of control groups 1-6, which shows that the sterilizing powder, the organic fertilizer I, the pretreatment of the cassia alata seeds, the pretreatment of the white clover and the interplanting method of the cassia alata and the white clover can effectively improve the growth capacity of the white clover and the cassia alata.

test run 3:

Single factor experiments were performed on cassia wingnut seed pretreatment: the cassia winged seed was divided equally into 9 groups and treated as per treatment as in table 3:

TABLE 3

After the seeds of the Cassia alata L.var.alata Maxim are treated according to the treatment method of the test group 1-9, the seeds are cultured for 7 days under the condition that the illumination intensity is 800Lx, the daily germination amount is counted at regular time every day from 1d after sowing, the germination is finished after 7d after sowing, the germination vigor and the germination rate are calculated, 3 seedlings are randomly selected for each treatment, the height and the main root length of the seedlings are measured by using a ruler, and the number of lateral roots is counted. See table 4 for details:

TABLE 4

As can be seen from the above table, the germination vigor, the germination rate, the seedling height, the main root length and the root number of the test groups 1-9 are all obviously lower than those of the control group, which indicates that the disinfectant, the seed soaking solution and the culture solution used in the application can obviously improve the germination vigor, the germination rate, the seedling height, the main root length and the root number of the cassia wingpod seedlings; wherein the disinfectant contains Aloe extract and willow bark extract as main active components; the main active component in the seed soaking liquid is okra extract; the culture solution contains main active ingredients including cassava residue soaking solution I, Spirulina extract and dragon fruit stem extract.

Test experiment 4:

carrying out single-factor test on the pretreatment of the Trifolium repens seeds: white clover seeds were evenly divided into 12 groups and treated as in table 5:

TABLE 5

After the white clover seeds are treated according to the treatment method of the test group 1-9, the white clover seeds are cultured for 7 days under the condition that the illumination intensity is 1000Lx, the daily germination amount is counted at regular time every day from 1d after sowing, the germination is finished after 7d after sowing, the germination vigor and the germination rate are calculated, 3 seedlings are randomly selected for each treatment, and the seedling height and the leaf integrity of the seedlings are measured by using a ruler. See table 6 for details:

TABLE 6

Group of	Germination vigor (%)	Percentage of germination (%)	seedling of young plantHigh (cm)	Leaf integrity (%)
					Test group 1	56.54	67.43	5.20	97.99
Test group 2	56.34	67.23	5.09	97.19
					test group 3	66.20	77.23	8.09	84.10
test group 4	58.34	69.23	5.95	97.99
					Test group 5	57.46	68.35	6.09	98.10
Test group 6	57.34	68.23	6.98	98.30
					Test group 7	56.34	67.23	6.42	98.32
test group 8	56.87	67.76	6.09	98.48
					test group 9	66.34	76.23	8.13	88.10
Test group 10	65.45	77.28	8.18	87.10
					test group 11	66.34	77.34	8.14	88.21
Test group 12	64.45	76.76	8.16	87.99
					Control group	67.43	78.32	9.07	99.08

As can be seen from the above table, the germination vigor, the germination rate and the seedling height of the test groups 1-2 and the test groups 4-8 are obviously lower than those of the control group, which indicates that the disinfectant, the seed soaking liquid and the culture solution used in the application can obviously improve the germination vigor, the germination rate, the seedling height, the main root length and the root number of the cassia wingpod seedlings; wherein the disinfectant contains Aloe extract and willow bark extract as main active components; the main active component in the seed soaking liquid is okra extract; the culture solution contains main active ingredients including cassava residue soaking solution I, spirulina extract and dragon fruit stem extract; the leaf integrity of the test group 3 and the test groups 9-12 is obviously lower than that of the control group, which shows that the insect repellent can play the role of repelling insects, and the orange peel extract, the eucalyptus extract, the willow bark extract and the lemon extract in the insect repellent are all absent, so that the leaf integrity of the white clover can be obviously improved.

In conclusion, the method of the invention can effectively improve the contents of nitrogen, phosphorus and organic matters in soil and improve the soil environment by interplanting the cassia alata and the white clover, and can also effectively reduce the content of arsenic to improve the soil, and a series of treatment methods are used in the interplanting process: the sterilization powder, the organic fertilizer I, the pretreatment of the Cassia alata seeds and the pretreatment of the white clover can effectively improve the germination rate of the Cassia alata seeds and accelerate the growth of the Cassia alata and the white clover; the insect repellant used in the application can obviously play a role in expelling insects and improve the integrity of the leaves.

The above examples are merely illustrative of several embodiments of the present invention, and the description thereof is more specific and detailed, but not to be construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. the interplanting method of the Cassia alata and the white clover is characterized by comprising the following steps:

(2) Pretreatment of Cassia alata seeds: selecting healthy and plump Cassia alata seeds, mixing the Cassia alata seeds with a disinfectant according to a mass ratio of 1:2-3, soaking for 5-10 min, washing with distilled water, soaking the Cassia alata seeds and a seed soaking liquid according to a mass ratio of 1:3-4 for 24-26 h at a constant temperature of 25-30 ℃, filtering after soaking, draining the seeds, uniformly placing the seeds in a culture cup I, and culturing under the conditions of a temperature of 25-30 ℃, a relative humidity of 10-15% and a light intensity of 800Lx-1000Lx until the Cassia alata seedlings with a height of 5-7 cm grow out; the washing mode of the distilled water is as follows: filtering Cassia alata seeds from a disinfectant, taking out, mixing the Cassia alata seeds and distilled water according to the mass ratio of 1:2, stirring for 1min, removing the distilled water, mixing the Cassia alata seeds and the distilled water according to the mass ratio of 1:2, stirring, and repeatedly washing for 5-7 times;

(4) Pretreatment of white clover seeds: selecting healthy and plump white clover seeds, mixing the white clover seeds with a germination accelerating solution according to a mass ratio of 1:2-3, soaking for 20-30 min, filtering, then embedding the white clover seeds in sandy soil, taking out the seeds after 12-14 h, washing the white clover seeds with distilled water for 5-7 min, draining the seeds, uniformly placing the drained seeds in a culture cup II, and culturing under the conditions that the temperature is 28-32 ℃, the relative humidity is 20-25%, and the illumination intensity is 1000Lx-1500Lx until white clover seedlings with the height of 5-8 cm grow out;

(5) interplanting white clover: planting the cassia tora alata seedlings pretreated in the step (4) between two adjacent rows of cassia tora alata 20-30 days after the cassia tora alata is planted in the step (3), and planting the seedlings according to the weight of 300g/m²-400g/m²spraying insect repellent into the whole planting land at the spraying amount, and spraying once again according to the same spraying amount after the interval of 20-30 d;

The disinfectant in the step (2) comprises the following components in percentage by weight: 0.1% -0.3% of HgCl₂1 to 3 percent of aloe extract, 2 to 5 percent of willow bark extract and the balance of water.

2. The method for interplanting cassia winged fruit and white clover according to claim 1, wherein the disinfectant powder in the step (1) comprises the following components in parts by weight: 15-25 parts of calcium oxide, 8-13 parts of eucalyptus extract, 9-15 parts of sweet wormwood extract, 7-13 parts of chrysanthemum root bark extract and 5-11 parts of chitosan.

3. the method for interplanting cassia winged fruit and white clover according to claim 1, wherein the organic fertilizer I in the step (1) is prepared from the following components in parts by weight: 5-10 parts of vermiculite, 15-25 parts of cassava dregs, 13-23 parts of mulberry leaf enzyme, 14-26 parts of soybean hulls and 23-35 parts of peanut bran.

4. The method for interplanting cassia winged fruit and white clover according to claim 1, wherein the seed soaking liquid in the step (2) comprises the following components in percentage by weight: 1 to 3 percent of potassium permanganate, 1 to 3 percent of gibberellin, 1 to 3 percent of sodium chloride, 1 to 3 percent of okra extract and the balance of water.

5. The method for interplanting cassia winged and white clover according to claim 1, wherein the culture cup I in the step (2) is further filled with a cassia winged culture solution, and the culture solution comprises the following components in percentage by weight: 5 to 7 percent of cassava residue soak solution I, 2 to 5 percent of trehalose, 1 to 3 percent of spirulina extract, 1 to 3 percent of dragon fruit stem extract and the balance of water.

6. The method for interplanting cassia winged fruit and white clover according to claim 1, wherein the germination accelerating solution in the step (4) comprises the following components in percentage by weight: 8-10% of water fertilizer, 1-3% of indoleacetic acid, 1-3% of camellia oleifera dreg soaking solution, 1-3% of trehalose and the balance of water.

7. The method for interplanting cassia winged and white clover according to claim 1, wherein a white clover culture solution is further filled in the culture cup II in the step (4), and the white clover culture solution comprises the following components in percentage by weight: 8-10% of fermentation bed waste padding soaking liquid, 2-4% of chitosan, 1-3% of spirulina extract, 1-3% of pawpaw extract and the balance of water.

8. The method for interplanting cassia winged Cassia and white clover according to claim 1, wherein the insect repellent in the step (5) comprises the following components in percentage by weight: 2 to 5 percent of orange peel extract, 2 to 5 percent of eucalyptus extract, 2 to 5 percent of willow bark extract, 1 to 3 percent of lemon extract, 1 to 3 percent of sodium chloride and the balance of water.