CN107771674A - Hall crabapple flower breeding method and its florescence control method - Google Patents
Hall crabapple flower breeding method and its florescence control method Download PDFInfo
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- CN107771674A CN107771674A CN201711124179.1A CN201711124179A CN107771674A CN 107771674 A CN107771674 A CN 107771674A CN 201711124179 A CN201711124179 A CN 201711124179A CN 107771674 A CN107771674 A CN 107771674A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a kind of hall crabapple flower breeding method and its florescence control method, belong to technical field of bioengineering, solve the problems, such as that traditional hall crabapple flower production yields is low, cost is high, be difficult to obtain popularity and currently without effective hall crabapple flower florescence control technology.It is main to describe two kinds of hall crabapple flower breeding methods and effective hall crabapple flower regulation and control method.Compared with traditional hall crabapple flower Cultivating techniques same period, production efficiency substantially increases substantially breeding method of the present invention；Florescence control method is simple and easy, and practical value is high, and economic results in society are obvious.
Description
Technical field
The invention belongs to technical field of bioengineering, specifically, more particularly to a kind of hall crabapple flower breeding method and its
Florescence control method.
Background technology
Hall crabapple flower scientific name Malus halliana Koehne.Tree crown is carried out, bark taupe, smooth.Sprout is purple brown
Color, there is sparsely grow pubescence, leaf alternate, elliptical or oval shape, tip is tapering, and edge sawtooth is tiny and blunt.The highest is spent, if bright when opening
Rosy clouds, umbrella shape raceme, blossom 4-7, bennet is elongated, sagging, red when not opening, and pink is faded to after opening, and is mostly half
Polyphyll, also there is simple flower.The month at florescence 3-4.The operatic circle obovate, yellow green, incidentally purple, fructescence September-October.Tree crown is evacuated,
Tree performance is whirling, and bennet is elongated, bud bright red, grows up, then sagging when open, and pollen is red, like last layer powder fat is smeared, preferably
Plant and most preferably planted in waterside on lawn in path both sides, or isolated planting, group planting.Fruit reddish yellow is alternate, exquisite lovely.Hall crabapple flower
With very high ornamental value, medical value and edibility, compatriots are enjoyed to like, but hall crabapple flower yield is relatively low,
Purchase cost is high, hardly results in commonly used.In addition, for the florescence control of hall crabapple flower, there is presently no effective side
Method.The florescence control of hall crabapple flower is significant, and from the angle of social benefit, the florescence for regulating and controlling hall crabapple flower can be market
Fresh flower product is provided on time, red-letter day is enriched or meets daily demand；From the angle of economic benefit, by the manual adjustment florescence,
Annual flower supply is fully able to, precise arrangements breeding program, the production cycle can be shortened, accelerates land use turnover rate, it is punctual for flower
The favourable market price can also be obtained.So research for hall crabapple flower florescence control technology or significant
's.
The content of the invention
The purpose of the present invention is in view of the deficienciess of the prior art, providing hall crabapple flower breeding method and its florescence tune
Prosecutor method.
The present invention is achieved by the following technical solutions：
A kind of hall crabapple flower breeding method, comprises the following steps：
A. in spring, the 3-4cm just sprouted hall crabapple flower bud is removed, aseptically with 75% alcohol disinfecting 30s, with
Afterwards with 0.1% mercuric chloride（HgC12）8-10min is sterilized, aseptic water washing is used after sterilization 4-5 times, material is cut into 0.8-1.0cm and grows section
Explant, aseptically it is inoculated on proliferated culture medium；
B. after explant described in step A is bred and stem apex, selection healthy and strong stem apex seedling, which is inoculated on root media, is given birth to
Root culture；
Preferably, the formula of the proliferated culture medium is MS+6-BA0.5 ~ 1.0mg/L+NAA0.1 ~ 0.3mg/L+20 ~ 30g sucrose
+ 6.5g agar.
Preferably, the proliferated culture medium pH value in the step A is adjusted to 5.8, and is 11kg/cm in pressure2, temperature be
20-25min is sterilized under 121 DEG C of environment.
Preferably, the formula of the root media in the step B is 1/2MS+IBA0.3mg/L+ sucrose 20g/L.
A kind of hall crabapple flower breeding method, comprises the following steps：
A. 48 hole disks are used, in annual 6-7 months, takes hall crabapple flower to give birth to semi-lignified branch conduct then and adopts bar；
B. bar will be adopted described in step A and is cut into the branch section formation cutting that length is 4-6 centimetres, upper blade is stayed, it is tender to remove lower end
Leaf, top 0.5 centimeters on bud are cut flat, and lower cut is cut into single horse ear shape；
C. the cutting in step B is put into standby in clear water；
D. the cutting got ready is put into 800 times of carbendazim solutions in time before cuttage and soaked 5 minutes, then with 500 mg/litres
The aqueous solution of indolebutyric acid dips in the cutting base portion 5-10 seconds；
E. per one cutting of cave cuttage, stromal surface is exposed as principle using lateral bud；
F. watered immediately after cuttage；
G. 1 1000 times of 50% carbendazim solution is sprayed weekly after inserting, and prevents cutting from rotting；
H. carry out a spray fertilizer within two weeks, spray 0.3% urea and 0.2% potassium dihydrogen phosphate；
I. such rule operation is until take root.
Preferably, the collection of the branch in the step A it is general in the morning 8 when before carry out.
Preferably, the formula of the matrix in the step E includes turf, perlite, wherein vermiculite, turf, perlite, leech
The proportioning of stone is 2:1:1；Or including turf, perlite, wherein turf, the proportioning of perlite are 3:1；Or including husky, wood
Bits, peat, wherein the proportioning of sand, wood chip, peat is 6:2:2.
A kind of hall crabapple flower florescence control method cultivated by the hall crabapple flower breeding method of claim 1 or 2, including carry
Preceding florescence control method and delay florescence control method；
The florescence control method comprises the following steps, and growth selection is healthy and strong, and the potted plant Jing Guo artificial shaping, October
The last ten-days period shift to an earlier date dormancy, are put into 0 DEG C of Freezing room；35 days removal freezers before the phase are opened in plan, under 5-10 DEG C of low temperature, daily on limb
Water spray 2-3 times；Greenhouse is moved into after 10 days, is heated up to 20-25 DEG C, can be buddingged within 10-15 days；10 DEG C of low temperature environments are placed in after buddingging
In, it is irradiated under the conditions of red blue colour mixture light light quality with the light of 40001x-70001x intensities of illumination, bud is developed the color；
The delay florescence control method comprises the following steps, and in late January to early Febuary, temperature not yet turns to warm up, bud is not sprouted
Before, 0-2 DEG C of freezer is moved into, continues to dormancy；Suitably watered when basin soil of 2 weekly check, overdrying, water can not be excessive；
Before the florescence is needed, when outdoor temperature is more than or equal to 20 DEG C, outbound in 10-15 days in advance, it is preferable to be firstly placed on ventilation condition under cool canopy
Place, bud sprout after be subjected to natural light irradiation, make pattern beautiful；Such as go out to budding too early, should be placed in 4 DEG C of freezer,
The florescence can be postponed；Such as budding relatively late, can go heating or spray 0.2% potassium dihydrogen phosphate 2-3 times again, promote to bloom.
Compared with prior art, the beneficial effects of the invention are as follows：
Compared with traditional hall crabapple flower Cultivating techniques same period, 1. production efficiency substantially increases substantially breeding method of the present invention, production
Amount is increased substantially so that the cost for buying hall crabapple flower is greatly lowered, and social and economic benefits increase substantially, and especially exist
Field of environment engineering obtains popularity；
2. the florescence control method of the present invention is simple to operation, the florescence for realizing hall crabapple flower with minimum regulatory process step adjusts
Control, practical value is high, and economic results in society are obvious.
Embodiment
The present invention is further described below：
Hall crabapple flower tree crown is carried out, bark taupe, smooth.Sprout puce, there is a sparsely grow pubescence, and leaf alternate is avette or ellipse
Circle, tip is tapering, and edge sawtooth is tiny and blunt.The highest is spent, if bright rosy clouds when opening, umbrella shape raceme, blossom 4-7, flower
Obstruct it is elongated, it is sagging, it is red when not opening, pink, mostly half polyphyll are faded to after opening, also there is simple flower.The month at florescence 3-4.The operatic circle
Obovate, yellow green, incidentally purple, fructescence September-October.Tree crown is evacuated, and tree performance is whirling, and bennet is elongated, bud bright red, to
Upper growth, then sagging when open, pollen is red, like last layer powder fat is smeared, preferably plants in path both sides, or isolated planting, group planting are in grass
On level ground, most preferably plant in waterside.Fruit reddish yellow is alternate, exquisite lovely.It is mainly used in viewing and admiring, is medicinal, edible.
A kind of hall crabapple flower breeding method, comprises the following steps：
A. in spring, the 3-4cm just sprouted hall crabapple flower bud is removed, aseptically with 75% alcohol disinfecting 30s, with
Afterwards with 0.1% mercuric chloride（HgC12）8-10min is sterilized, aseptic water washing is used after sterilization 4-5 times, material is cut into 0.8-1.0cm and grows section
Explant, aseptically it is inoculated on proliferated culture medium；
B. after explant described in step A is bred and stem apex, selection healthy and strong stem apex seedling, which is inoculated on root media, is given birth to
Root culture；
Preferably, the formula of the proliferated culture medium is MS+6-BA0.5 ~ 1.0mg/L+NAA0.1 ~ 0.3mg/L+20 ~ 30g sucrose
+ 6.5g agar.
Preferably, the proliferated culture medium pH value in the step A is adjusted to 5.8, and is 11kg/cm in pressure2, temperature be
20-25min is sterilized under 121 DEG C of environment.
Preferably, the formula of the root media in the step B is 1/2MS+IBA0.3mg/L+ sucrose 20g/L.
Wherein 6-BA Chinese scientific name is 6- benzyl aminopterin-induced syndromes；NAA Chinese scientific name is methyl α-naphthyl acetate；IBA Chinese scientific name
For heteroauxin.
A kind of hall crabapple flower breeding method, comprises the following steps：
A. 48 hole disks are used, in annual 6-7 months, takes hall crabapple flower to give birth to semi-lignified branch conduct then and adopts bar；
B. bar will be adopted described in step A and is cut into the branch section formation cutting that length is 4-6 centimetres, upper blade is stayed, it is tender to remove lower end
Leaf, top 0.5 centimeters on bud are cut flat, and lower cut is cut into single horse ear shape；
C. the cutting in step B is put into standby in clear water；
D. the cutting got ready is put into 800 times of carbendazim solutions in time before cuttage and soaked 5 minutes, then with 500 mg/litres
The aqueous solution of indolebutyric acid dips in the cutting base portion 5-10 seconds；
E. per one cutting of cave cuttage, stromal surface is exposed as principle using lateral bud；
F. watered immediately after cuttage；
G. 1 1000 times of 50% carbendazim solution is sprayed weekly after inserting, and prevents cutting from rotting；
H. carry out a spray fertilizer within two weeks, spray 0.3% urea and 0.2% potassium dihydrogen phosphate；
I. such rule operation is until take root.
Preferably, the collection of the branch in the step A it is general in the morning 8 when before carry out.
Preferably, the formula of the matrix in the step E includes turf, perlite, wherein vermiculite, turf, perlite, leech
The proportioning of stone is 2:1:1；Or including turf, perlite, wherein turf, the proportioning of perlite are 3:1；Or including husky, wood
Bits, peat, wherein the proportioning of sand, wood chip, peat is 6:2:2.
A kind of hall crabapple flower florescence control method cultivated by the hall crabapple flower breeding method of claim 1 or 2, including carry
Preceding florescence control method and delay florescence control method；
The florescence control method comprises the following steps, and growth selection is healthy and strong, and the potted plant Jing Guo artificial shaping, October
The last ten-days period shift to an earlier date dormancy, are put into 0 DEG C of Freezing room；35 days removal freezers before the phase are opened in plan, under 5-10 DEG C of low temperature, daily on limb
Water spray 2-3 times；Greenhouse is moved into after 10 days, is heated up to 20-25 DEG C, can be buddingged within 10-15 days；10 DEG C of low temperature environments are placed in after buddingging
In, it is irradiated under the conditions of red blue colour mixture light light quality with the light of 40001x-70001x intensities of illumination, bud is developed the color；
The delay florescence control method comprises the following steps, and in late January to early Febuary, temperature not yet turns to warm up, bud is not sprouted
Before, 0-2 DEG C of freezer is moved into, continues to dormancy；Suitably watered when basin soil of 2 weekly check, overdrying, water can not be excessive；
Before the florescence is needed, when outdoor temperature is more than or equal to 20 DEG C, outbound in 10-15 days in advance, it is preferable to be firstly placed on ventilation condition under cool canopy
Place, bud sprout after be subjected to natural light irradiation, make pattern beautiful；Such as go out to budding too early, should be placed in 4 DEG C of freezer,
The florescence can be postponed；Such as budding relatively late, can go heating or spray 0.2% potassium dihydrogen phosphate 2-3 times again, promote to bloom.
Embodiment 1：
Hall crabapple flower breeding method, comprises the following steps：
A. in spring, the 3-4cm just sprouted hall crabapple flower bud is removed, aseptically with 75% alcohol disinfecting 30s, with
Afterwards with 0.1% mercuric chloride（HgC12）8-10min is sterilized, aseptic water washing is used after sterilization 4-5 times, material is cut into 0.8-1.0cm and grows section
Explant, aseptically it is inoculated on proliferated culture medium；
B. after explant described in step A is bred and stem apex, selection healthy and strong stem apex seedling, which is inoculated on root media, is given birth to
Root culture；
The formula of the proliferated culture medium is MS+6-BA0.5 ~ 1.0mg/L+NAA0.1 ~ 0.3mg/L+20 ~ 30g sucrose+6.5g fine jades
Fat；The proliferated culture medium pH value in the step A is adjusted to 5.8, and is 11kg/cm in pressure2, temperature be under 121 DEG C of environment
Sterilize 20-25min.The formula of proliferated culture medium in the step B is 1/2MS+IBA0.3mg/L+ sucrose 20g/L.
By spring, the 3-4cm just sprouted hall crabapple flower bud is removed, aseptically with 75% alcohol disinfecting 30s,
Then with 0.1% mercuric chloride（HgC12）8-10min is sterilized, aseptic water washing is used after sterilization 4-5 times, material is cut into 0.8-1.0cm length
Section explant, is aseptically inoculated on proliferated culture medium, the formula of the proliferated culture medium is MS+6-BA0.5 ~ 1.0mg/
L+NAA0.1 ~ 0.3mg/L+20 ~ 30g sucrose+6.5g agar.And the proliferated culture medium pH value need to be adjusted to 5.8, and it is in pressure
11kg/cm2, temperature be 121 DEG C of environment under sterilize 20-25min.After the explant is bred and stem apex, healthy and strong stem apex is chosen
Seedling, which is inoculated on root media, carries out culture of rootage, and the formula of root media is 1/2MS+IBA0.3mg/L+ sucrose 20g/
L, in the generation that 15 days or so just have root, rooting rate is up to more than 95% after 30 days.
Wherein, test result indicates that, proliferation culture medium formula be MS+6-BA0.5mg/L+NAA0.1mg/L+20g sucrose+
During 6.5g agar, growth coefficient is up to 5；Proliferation culture medium formula be MS+6-BA0.6mg/L+NAA0.2mg/L+20g sucrose+
During 6.5g agar, growth coefficient is up to 5.5；Proliferation culture medium formula be MS+6-BA0.7mg/L+NAA0.3mg/L+30g sucrose+
During 6.5g agar, growth coefficient is up to 7；Proliferation culture medium formula be MS+6-BA1.0mg/L+NAA0.3mg/L+30g sucrose+
During 6.5g agar, growth coefficient occurs vitrification phenomenon and influences the later stage and continue to break up up to 9, therefore breeds Nutrient medium
It is the optimum culture medium of Shoot Tip Culture base to be formulated MS+6-BA1.0mg/L+NAA0.3mg/L+30g sucrose+6.5g agar.
For the breeding method of the embodiment of the present invention 1 compared with traditional hall crabapple flower Cultivating techniques same period, production efficiency improves 60-
80%.
Embodiment 2：
Hall crabapple flower breeding method, comprises the following steps：
A. 48 hole disks are used, in annual 6-7 months, takes hall crabapple flower to give birth to semi-lignified branch conduct then and adopts bar；
B. bar will be adopted described in step A and is cut into the branch section formation cutting that length is 4-6 centimetres, upper blade is stayed, it is tender to remove lower end
Leaf, top 0.5 centimeters on bud are cut flat, and lower cut is cut into single horse ear shape；
C. the cutting in step B is put into standby in clear water；
D. the cutting got ready is put into 800 times of carbendazim solutions in time before cuttage and soaked 5 minutes, then with 500 mg/litres
The aqueous solution of indolebutyric acid dips in the cutting base portion 5-10 seconds；
E. per one cutting of cave cuttage, stromal surface is exposed as principle using lateral bud；
F. watered immediately after cuttage；
G. 1 1000 times of 50% carbendazim solution is sprayed weekly after inserting, and prevents cutting from rotting；
H. carry out a spray fertilizer within two weeks, spray 0.3% urea and 0.2% potassium dihydrogen phosphate；
I. such rule operation is until take root.
Preferably, the collection of the branch in the step A it is general in the morning 8 when before carry out.
Preferably, the formula of the matrix in the step E includes turf, perlite, wherein vermiculite, turf, perlite, leech
The proportioning of stone is 2:1:1；Or including turf, perlite, wherein turf, the proportioning of perlite are 3:1；Or including husky, wood
Bits, peat, wherein the proportioning of sand, wood chip, peat is 6:2:2.
Using 48 hole disks, in annual 6-7 months, take hall crabapple flower to give birth to semi-lignified branch conduct then and adopt bar.Branch
The collection of bar it is general in the morning 8 when before carry out.The bar of adopting is cut into the branch section formation cutting that length is 4-6 centimetres, stayed
Portion's blade, remove lower end tender leaf, top 0.5 centimeters on bud are cut flat, and lower cut is cut into single horse ear shape.Cutting is put into afterwards
It is standby in clear water.The cutting got ready is put into 800 times of carbendazim solutions in time before cuttage and soaked 5 minutes, then with 500 millis
G/l the aqueous solution of indolebutyric acid dip in the cutting base portion 5-10 seconds.Per cave cuttage one cutting, using lateral bud expose stromal surface as
Principle.Matching somebody with somebody for its mesostroma can be three kinds, and the first formula includes turf, perlite, vermiculite, wherein turf, perlite, leech
The proportioning of stone is 2:1:1；Second of formula includes turf, perlite, and wherein turf, the proportioning of perlite are 3:1；The third is matched somebody with somebody
Side includes sand, wood chip, peat, wherein the proportioning of sand, wood chip, peat is 6:2:2.Watered immediately after cuttage, start auto spraying
Device, initial stage, spray amount was big, and interval time is short, and mid-term is suitably sprayed less, later stage control water hardening.Insert after spray weekly 1 time 1000 times
50% carbendazim solution, prevents cutting from rotting.Carry out a spray fertilizer within two weeks, spray 0.3% urea and 0.2% potassium dihydrogen phosphate.15
Base portion starts block protuberance occur after it, starts to take root after 20 days, peak of taking root is reached after 30 days, rooting rate is reachable after 40 days
90%.
For the breeding method of the embodiment of the present invention 1 compared with traditional hall crabapple flower Cultivating techniques same period, production efficiency improves 53-
70%.
Embodiment 3：
Hall crabapple flower florescence control method, including shift to an earlier date florescence control method and delay florescence control method；The florescence control
Method comprises the following steps, and growth selection is healthy and strong, and the potted plant Jing Guo artificial shaping, shifts to an earlier date dormancy in late October, puts
Enter 0 DEG C of Freezing room；35 days removal freezers before the phase are opened in plan, under 5-10 DEG C of low temperature, daily in being sprayed water 2-3 times on limb；10 days
After move into greenhouse, be heated up to 20-25 DEG C, can budding within 10-15 days；It is placed in after buddingging in 10 DEG C of low temperature environments, in red blue colour mixture
It is irradiated under the conditions of light light quality with the light of 40001x-70001x intensities of illumination, bud is developed the color；The delay florescence control
Method comprises the following steps, and in late January to early Febuary, before temperature not yet turns to warm up, bud is not sprouted, moves into 0-2 DEG C of freezer, makes
It continues dormancy；Suitably watered when basin soil of 2 weekly check, overdrying, water can not be excessive；Before the florescence is needed, work as outdoor temp
When degree is more than or equal to 20 DEG C, outbound in 10-15 days in advance is firstly placed on that ventilation condition under cool canopy is preferably local, and bud can after sprouting
Receive natural light irradiation, make pattern beautiful；Such as go out to budding too early, should be placed in 4 DEG C of freezer, the florescence can be postponed；Such as budding compared with
Late, it can again go and heating or spray with 0.2% potassium dihydrogen phosphate 2-3 times, promote to bloom.The florescence control method of the present invention is simply easily grasped
Make, the florescence control of hall crabapple flower is realized with minimum regulatory process step, practical value is high, and economic results in society are obvious.
In summary, only presently preferred embodiments of the present invention, is not used for limiting the scope that the present invention is implemented, it is all according to
The equivalent changes and modifications carried out by shape, construction, feature and spirit described in scope of the invention as claimed, this all should be included in
In the right of invention.
Claims (7)
1. a kind of hall crabapple flower breeding method, it is characterised in that comprise the following steps：
A. in spring, the 3-4cm just sprouted hall crabapple flower bud is removed, aseptically with 75% alcohol disinfecting 30s, with
Afterwards with 0.1% mercuric chloride（HgC12）8-10min is sterilized, aseptic water washing is used after sterilization 4-5 times, material is cut into 0.8-1.0cm and grows section
Explant, aseptically it is inoculated on proliferated culture medium；
B. after explant described in step A is bred and stem apex, selection healthy and strong stem apex seedling, which is inoculated on root media, is given birth to
Root culture；
Hall crabapple flower breeding method according to claim 1, it is characterised in that：The formula of the proliferated culture medium is MS+
6-BA0.5 ~ 1.0mg/L+NAA0.1 ~ 0.3mg/L+20 ~ 30g sucrose+6.5g agar.
2. hall crabapple flower breeding method according to claim 1 or 2, it is characterised in that：Adjust the propagation in the step A
Medium's PH Value is 11kg/cm to 5.8, and in pressure2, temperature be 121 DEG C of environment under sterilize 20-25min.
3. hall crabapple flower breeding method according to claim 1, it is characterised in that：Root media in the step B
Formula be 1/2MS+IBA0.3mg/L+ sucrose 20g/L.
4. a kind of hall crabapple flower breeding method, it is characterised in that comprise the following steps：
A. 48 hole disks are used, in annual 6-7 months, takes hall crabapple flower to give birth to semi-lignified branch conduct then and adopts bar；
B. bar will be adopted described in step A and is cut into the branch section formation cutting that length is 4-6 centimetres, upper blade is stayed, it is tender to remove lower end
Leaf, top 0.5 centimeters on bud are cut flat, and lower cut is cut into single horse ear shape；
C. the cutting in step B is put into standby in clear water；
D. the cutting got ready is put into 800 times of carbendazim solutions in time before cuttage and soaked 5 minutes, then with 500 mg/litres
The aqueous solution of indolebutyric acid dips in the cutting base portion 5-10 seconds；
E. per one cutting of cave cuttage, stromal surface is exposed as principle using lateral bud；
F. watered immediately after cuttage；
G. 1 1000 times of 50% carbendazim solution is sprayed weekly after inserting, and prevents cutting from rotting；
H. carry out a spray fertilizer within two weeks, spray 0.3% urea and 0.2% potassium dihydrogen phosphate；
I. such rule operation is until take root.
5. hall crabapple flower breeding method according to claim 2, it is characterised in that：The collection of branch in the step A
Typically in the morning 8 when before carry out.
6. hall crabapple flower breeding method according to claim 2, it is characterised in that：The formula of matrix in the step E
Including turf, perlite, vermiculite, wherein turf, perlite, the proportioning of vermiculite are 2:1:1；Or including turf, perlite, its
Middle turf, the proportioning of perlite are 3:1；Or including sand, wood chip, peat, wherein the proportioning of sand, wood chip, peat is 6:2:2.
7. a kind of hall crabapple flower florescence control method cultivated by the hall crabapple flower breeding method of claim 1 or 2, its feature exist
In：Including shifting to an earlier date florescence control method and delay florescence control method；
The florescence control method comprises the following steps, and growth selection is healthy and strong, and the potted plant Jing Guo artificial shaping, October
The last ten-days period shift to an earlier date dormancy, are put into 0 DEG C of Freezing room；35 days removal freezers before the phase are opened in plan, under 5-10 DEG C of low temperature, daily on limb
Water spray 2-3 times；Greenhouse is moved into after 10 days, is heated up to 20-25 DEG C, can be buddingged within 10-15 days；10 DEG C of low temperature environments are placed in after buddingging
In, it is irradiated under the conditions of red blue colour mixture light light quality with the light of 40001x-70001x intensities of illumination, bud is developed the color；
The delay florescence control method comprises the following steps, and in late January to early Febuary, temperature not yet turns to warm up, bud is not sprouted
Before, 0-2 DEG C of freezer is moved into, continues to dormancy；Suitably watered when basin soil of 2 weekly check, overdrying, water can not be excessive；
Before the florescence is needed, when outdoor temperature is more than or equal to 20 DEG C, outbound in 10-15 days in advance, it is preferable to be firstly placed on ventilation condition under cool canopy
Place, bud sprout after be subjected to natural light irradiation, make pattern beautiful；Such as go out to budding too early, should be placed in 4 DEG C of freezer,
The florescence can be postponed；Such as budding relatively late, can go heating or spray 0.2% potassium dihydrogen phosphate 2-3 times again, promote to bloom.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711124179.1A CN107771674A (en) | 2017-11-14 | 2017-11-14 | Hall crabapple flower breeding method and its florescence control method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711124179.1A CN107771674A (en) | 2017-11-14 | 2017-11-14 | Hall crabapple flower breeding method and its florescence control method |
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| CN107771674A true CN107771674A (en) | 2018-03-09 |
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Citations (3)
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| CN103749137A (en) * | 2014-01-16 | 2014-04-30 | 四川农业大学 | Cutting seedling method for malus toringoides |
| CN103875498A (en) * | 2014-03-10 | 2014-06-25 | 宣威市三友农业科技发展有限公司 | Method for planting malus halliana |
| CN104686331A (en) * | 2015-02-21 | 2015-06-10 | 杨业云 | Tissue culture and rapid propagation method for malus halliana |
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|---|---|---|---|---|
| CN103749137A (en) * | 2014-01-16 | 2014-04-30 | 四川农业大学 | Cutting seedling method for malus toringoides |
| CN103875498A (en) * | 2014-03-10 | 2014-06-25 | 宣威市三友农业科技发展有限公司 | Method for planting malus halliana |
| CN104686331A (en) * | 2015-02-21 | 2015-06-10 | 杨业云 | Tissue culture and rapid propagation method for malus halliana |
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