CN107743837A - A kind of method for breeding Chinese cabbage self incompatible line - Google Patents
A kind of method for breeding Chinese cabbage self incompatible line Download PDFInfo
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- CN107743837A CN107743837A CN201711014358.XA CN201711014358A CN107743837A CN 107743837 A CN107743837 A CN 107743837A CN 201711014358 A CN201711014358 A CN 201711014358A CN 107743837 A CN107743837 A CN 107743837A
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- Prior art keywords
- greenhouse
- chinese cabbage
- small plastic
- plastic shed
- self incompatible
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- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 title claims abstract description 32
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 title claims abstract description 31
- 238000009395 breeding Methods 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 27
- 230000001488 breeding effect Effects 0.000 title claims abstract description 26
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 title claims abstract 12
- 239000003337 fertilizer Substances 0.000 claims description 9
- 241000256844 Apis mellifera Species 0.000 claims description 8
- 241000196324 Embryophyta Species 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 238000009413 insulation Methods 0.000 claims description 3
- 238000005259 measurement Methods 0.000 claims description 3
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims description 2
- 238000003306 harvesting Methods 0.000 claims 1
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 abstract description 11
- 230000010211 insect pollination Effects 0.000 abstract description 2
- 230000005849 recognition of pollen Effects 0.000 abstract description 2
- 238000012216 screening Methods 0.000 abstract description 2
- 244000221633 Brassica rapa subsp chinensis Species 0.000 description 20
- 238000012545 processing Methods 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000010152 pollination Effects 0.000 description 2
- 235000011331 Brassica Nutrition 0.000 description 1
- 241000219198 Brassica Species 0.000 description 1
- 235000011292 Brassica rapa Nutrition 0.000 description 1
- 240000008100 Brassica rapa Species 0.000 description 1
- 241000219193 Brassicaceae Species 0.000 description 1
- 206010021929 Infertility male Diseases 0.000 description 1
- 208000007466 Male Infertility Diseases 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000009405 line breeding Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/02—Treatment of plants with carbon dioxide
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of method for breeding Chinese cabbage self incompatible line, this method utilizes high concentration CO in self incompatible line florescence2Long time treatment, break its self-incompatibility, overcome it to be selfed the difficulty of breeding, and combine CO2Automatic release, insect pollination, later stage screening etc. can efficiently carry out the breeding work of Chinese cabbage self incompatible line, Chinese cabbage is promoted to be selfed the quick utilization of not affine hybrid, it is difficult to overcome a large amount of breedings of Chinese cabbage self incompatible line in the prior art, so as to limit the problem of not affine hybrid of selfing is quickly applied.
Description
Technical field
The invention belongs to Chinese cabbage breeding field, specific to the skill of Chinese cabbage self incompatible line breeding volume increase
Art method.
Background technology
Chinese cabbage (Brassica rapa L.Chinensis Group) is commonly called as green vegetables, pakchoi, is Cruciferae
The Constantly allogamous plant of Brassica genus, there is significant hybrid vigour.Chinese cabbage is mainly passed through using hybrid vigour at present
Male sterility and the not affine two methods of selfing, wherein being main method using self incompatible line seed selection new varieties, selfing is not
Affine system's seed selection new varieties have the characteristics that the seed selection cycle is short, high without bad cytoplasmic effect, breeding yield.
The method that the selfing breeding of Chinese cabbage self incompatible line mainly uses includes artificial cabage-type rape method, electricity helps
Pollination method, heat help pollination method and NaCl solution method etc., these methods are either time consuming, cost is high, demand strict technology or
Affected by environment, hybrid seed yield is unstable.The breeding of Chinese cabbage self incompatible line have impact on Chinese cabbage selfing not
The heterosis utilization of affine system.High concentration CO is utilized under the manual control condition of facility plastic greenhouse2Break Chinese cabbage certainly
The self-incompatibility of not affine system is handed over, this process can realize automation, artificial so as to reduce.High concentration CO 2 processing procedure
In induction of the expression for being selfed not affine related gene, not by environmental influences such as temperature, illumination, so as to have stable yield
The advantages that.
The content of the invention
The invention provides a kind of a large amount of breeding methods of Chinese cabbage self incompatible line, the purpose of this method is
The defects of overcoming prior art, simple efficient cost is high when solving breeding Chinese cabbage self incompatible line, yields poorly
Problem, Chinese cabbage is accelerated to be selfed the utilization of not affine hybrid.
The purpose of the present invention can be achieved through the following technical solutions：
A kind of method for breeding Chinese cabbage self incompatible line, is comprised the steps of：
(1) before planting strain field planting, facility plastic greenhouse is transformed, and Small plastic shed is built in greenhouse；
(2) kind strain is colonized in Small plastic shed, is colonized using size row during field planting, is advantageous to divulge information, mitigates the generation of disease；
(3) Small plastic shed capping canopy film, canopy film surrounding need to reserve at least 0.5 meter more；Greenhouse leaks informaton daytime, night insulation, most
When low temperature is less than 5 DEG C, Small plastic shed is incubated for night；Control water after kind strain work, prevents disease；
(4) before spring bolting, placement CO in Small plastic shed2Concentration sensor and CO2Discharge tube, greenhouse are capped fly net outside；
(5) operation room after the early flowering season between greenhouse and Small plastic shed, which is placed, includes bee box and CO2Phase including gas cylinder
Close control device；
(6) after self incompatible line enters initial bloom stage, treat that honeybee returns locking honeycomb after nest at dusk daily, by Small plastic shed canopy
Film is put down, and surrounding compacting, is ensured closed；
(7) early morning 6 on next day:30~7：00, greenhouse is opened, then carries out CO2Fertilizer treatment, it is desirable to CO in Small plastic shed2Concentration
Maintain 4%~4.5%, CO2Small plastic shed is raised after 3~5 hours fertilizer treatment time, then opens honeycomb；
(8) CO is terminated within 8~12 days after full-bloom stage2Discharge, include bee box and CO between dismantling operation2Phase including gas cylinder
Close control device；Raised always after Small plastic shed, kind strain carries out normal management；
(9) after the florescence terminates, field few plant of bearing pods is pulled out superseded by breeding personnel, and mortality is 1~2%, kind
Harvested in time after sub- maturation.
As a kind of optimal technical scheme, the mode transformed in step (1) facility plastic greenhouse is：In greenhouse porch
Reserved 2~4 meters operation room, builds semicircle Small plastic shed in greenhouse afterwards, and the height of Small plastic shed meets kind of a height for strain growth
Degree requires.
As a kind of optimal technical scheme, when being colonized in step (2) using size row, small 30~35cm of line space, big row
45~50cm, spacing in the rows are normal.
As a kind of optimal technical scheme, CO described in step (4)2The measurement range of concentration sensor is at least up to
50000PPM, per 10m2Installation one.
As a kind of optimal technical scheme, free of frost weather can open greenhouse and stay overnight in step (6).
As a kind of optimal technical scheme, CO described in step (7)2The time of fertilizer treatment is 4 hours.
As a kind of optimal technical scheme, 10 days after full-bloom stage in step (8) terminate CO2Discharge.
This method utilizes high concentration CO in self incompatible line florescence2Long time treatment, it is not affine to break its selfing
Property, overcome it to be selfed the difficulty of breeding, and combine CO2Automatic release, insect pollination, later stage screening etc. can be carried out efficiently not
The breeding work of Chinese cabbage self incompatible line, promotes Chinese cabbage to be selfed the quick utilization of not affine hybrid, overcomes
The a large amount of breedings of Chinese cabbage self incompatible line are difficult in the prior art, quickly applied so as to limit the not affine hybrid of selfing
The problem of.
The advantage of the invention is that：
Utilize high concentration CO2The process of processing can realize automation, saved compared to other methods manually, independent of behaviour
Make the experience of personnel, yield is significantly increased and reduces the restriction of environment.
Embodiment
The content of the invention of the present invention is described further with reference to embodiment.
Embodiment 1 breeds Chinese cabbage self incompatible line
(1) according to the mode of big strain breeding, In Nanjing field planting kind mid-November strain.It is complete before in mid or late October, 2016
Into greenhouse site preparation and transformation.Local common 8332 greenhouses (greenhouse wide 8 meters, rising 3.2 meters, shoulder height 1.8) of selection, greenhouse length
90 meters.Semicircle Small plastic shed is built in greenhouse, wide 6 meters, both sides respectively retain 1 meter, rise 2.5 meters, 1.6 meters of shoulder height, in Small plastic shed
Can tractor-ploughing site preparation, stay aisle, about 2 meters of furrow face width among duplicate rows.85 meters of Small plastic shed length, greenhouse are entered and stay 3 meters of works at canopy head
Operation room, 2 meters are stayed at greenhouse dingle, convenient operation.
(2) free Chinese cabbage self incompatible line SI1000 is selected mid-November in kind of strain field planting Small plastic shed, is adopted
It is colonized with size row, spacing in the rows 30cm, small line space 30cm, big line space 50cm, to be advantageous to divulge information, mitigates the generation of disease.
(3) Small plastic shed capping canopy film, canopy film is wide 10 meters, long 92 meters, and canopy film surrounding needs to reserve 0.5 meter more.Greenhouse is put daytime
Wind, night close insulation.When the lowest temperature is less than 5 DEG C, Small plastic shed just can be used for night to be incubated.Control water after kind strain work, prevents
Only survive the winter soft corruption.
(4) before spring bolting, CO is installed in the operation room that greenhouse head is reserved2(one kind automatically controls automatic applicator
CO2 gas fertilizer applicators, the U of Authorization Notice No. CN 203167709), and by CO2Concentration sensor and CO2Discharge tube is placed in
In Small plastic shed, it is desirable to CO2Concentration sensor measurement range is at least up to 50000PPM, and every 10 ㎡ installs one.It is capped outside greenhouse
Fly net.
(5) bee box, CO are placed in operation room after the early flowering season2The control device of gas cylinder and correlation.
(6) self incompatible line SI1000 carries out CO after entering initial bloom stage2Discharge, treat that honeybee is locked after returning nest at dusk daily
Honeycomb is closed, Small plastic shed canopy film is put down, surrounding is compacted as far as possible, is ensured closed.Free of frost weather can open greenhouse and stay overnight.
(7) early morning 6 on next day:30~7：00, greenhouse is opened, then carries out CO2Fertilizer treatment, it is desirable to CO in Small plastic shed2Concentration
Maintenance 4%~4.5%, after 4 hours processing times, Small plastic shed is raised, then opens honeycomb.
(8) CO is terminated within 10 days after full-bloom stage2Fertilizer treatment, the relevant device between dismantling operation.After Small plastic shed always
Raise, kind strain carries out normal management.
(9) after the florescence terminates, field less plant of bearing pods is pulled out superseded, mortality about 1~2% by breeding personnel,
Harvested in time after seed maturity.The Chinese cabbage self incompatible line SI1000 yield of spring in 2017 is 29.7kg/667M2。
Finally illustrate, the above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted, although with reference to compared with
The present invention is described in detail good embodiment, it will be understood by those within the art that, can be to the skill of the present invention
Art scheme is modified or equivalent substitution, and without departing from the objective and scope of technical solution of the present invention, it all should cover at this
Among the right of invention.
Claims (7)
- A kind of 1. method for breeding Chinese cabbage self incompatible line, it is characterised in that：Comprise the steps of：(1) before planting strain field planting, greenhouse is transformed, and Small plastic shed is built in greenhouse；(2) kind strain is colonized in Small plastic shed, is colonized using size row during field planting；(3) Small plastic shed capping canopy film, canopy film surrounding need to reserve at least 0.5 meter more；Greenhouse leaks informaton daytime, night insulation, minimum gas When temperature is less than 5 DEG C, Small plastic shed is incubated for night；Control water after kind strain work, prevents disease；(4) before spring bolting, placement CO in Small plastic shed2Concentration sensor and CO2Discharge tube, greenhouse are capped fly net outside；(5) operation room after the early flowering season between greenhouse and Small plastic shed, which is placed, includes bee box and CO2Control including gas cylinder is set It is standby；(6) after self incompatible line enters initial bloom stage, treat that honeybee returns locking honeycomb after nest at dusk daily, Small plastic shed canopy film is put Under, surrounding compacting, ensure closed；(7) early morning 6 on next day:30~7：00, greenhouse is opened, then carries out CO2Fertilizer treatment, it is desirable to CO in Small plastic shed2Concentration maintains 4%~4.5%, CO2Small plastic shed is raised after 3~5 hours fertilizer treatment time, then opens honeycomb；(8) CO is terminated within 8~12 days after full-bloom stage2Discharge, include bee box and CO between dismantling operation2Control including gas cylinder is set It is standby；Raised always after Small plastic shed, kind strain carries out normal management；(9) after the florescence terminates, field few plant of bearing pods is pulled out superseded by breeding personnel, and mortality is 1~2%, seed into Harvesting in time after ripe.
- 2. the method for breeding Chinese cabbage self incompatible line according to claim 1, it is characterised in that：Step (1) In the mode transformed facility plastic greenhouse be：In reserved 2~4 meters operation room in greenhouse porch, built afterwards in greenhouse Semicircle Small plastic shed, the height of Small plastic shed meet kind of a requirement for height for strain growth.
- 3. the method for breeding Chinese cabbage self incompatible line according to claim 1, it is characterised in that：Step (2) During the middle field planting using size row, small 30~35cm of line space, big 45~50cm of row.
- 4. the method for breeding Chinese cabbage self incompatible line according to claim 1, it is characterised in that：Step (4) Described in CO2The measurement range of concentration sensor is at least up to 50000PPM, per 10m2Installation one.
- 5. the method for breeding Chinese cabbage self incompatible line according to claim 1, it is characterised in that：Step (6) Middle free of frost weather can open greenhouse and stay overnight.
- 6. the method for breeding Chinese cabbage self incompatible line according to claim 1, it is characterised in that：Step (7) Described in CO2The time of fertilizer treatment is 4 hours.
- 7. the method for breeding Chinese cabbage self incompatible line according to claim 1, it is characterised in that：Step (8) Terminate CO within 10 days after middle full-bloom stage2Discharge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711014358.XA CN107743837B (en) | 2017-10-25 | 2017-10-25 | Method for breeding self-incompatible line of non-heading Chinese cabbage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711014358.XA CN107743837B (en) | 2017-10-25 | 2017-10-25 | Method for breeding self-incompatible line of non-heading Chinese cabbage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107743837A true CN107743837A (en) | 2018-03-02 |
| CN107743837B CN107743837B (en) | 2020-05-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711014358.XA Expired - Fee Related CN107743837B (en) | 2017-10-25 | 2017-10-25 | Method for breeding self-incompatible line of non-heading Chinese cabbage |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109197513A (en) * | 2018-10-19 | 2019-01-15 | 南京市蔬菜科学研究所 | A kind of method of Chinese cabbage kind strain cultivation |
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| CN1148932A (en) * | 1996-10-03 | 1997-05-07 | 北京化工实验厂 | Method for supplying carbon dioxide gas fertilize to crops |
| CN1875691A (en) * | 2006-07-21 | 2006-12-13 | 吕平 | A plant quick propagation method |
| CN101422115A (en) * | 2008-12-09 | 2009-05-06 | 华中农业大学 | Method for propagating cabbage type rape of self incompatible lin |
| CN202406610U (en) * | 2011-12-31 | 2012-09-05 | 南京良华农业科技发展有限公司 | Special device utilizing fermentation of wastes to increase CO2 application to greenhouse vegetables |
| CN103039248A (en) * | 2013-01-08 | 2013-04-17 | 霍邱县红土地蔬果种植专业合作社 | Novel greenhouse strawberry culturing and planting method |
| US20130298293A1 (en) * | 2005-11-22 | 2013-11-07 | Seminis Vegetables Seeds, Inc. | Broccoli type having curds with detached florets |
| CN105917945A (en) * | 2016-05-25 | 2016-09-07 | 杨建冬 | Method for planting strawberries in greenhouse |
-
2017
- 2017-10-25 CN CN201711014358.XA patent/CN107743837B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1148932A (en) * | 1996-10-03 | 1997-05-07 | 北京化工实验厂 | Method for supplying carbon dioxide gas fertilize to crops |
| US20130298293A1 (en) * | 2005-11-22 | 2013-11-07 | Seminis Vegetables Seeds, Inc. | Broccoli type having curds with detached florets |
| CN1875691A (en) * | 2006-07-21 | 2006-12-13 | 吕平 | A plant quick propagation method |
| CN101422115A (en) * | 2008-12-09 | 2009-05-06 | 华中农业大学 | Method for propagating cabbage type rape of self incompatible lin |
| CN202406610U (en) * | 2011-12-31 | 2012-09-05 | 南京良华农业科技发展有限公司 | Special device utilizing fermentation of wastes to increase CO2 application to greenhouse vegetables |
| CN103039248A (en) * | 2013-01-08 | 2013-04-17 | 霍邱县红土地蔬果种植专业合作社 | Novel greenhouse strawberry culturing and planting method |
| CN105917945A (en) * | 2016-05-25 | 2016-09-07 | 杨建冬 | Method for planting strawberries in greenhouse |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109197513A (en) * | 2018-10-19 | 2019-01-15 | 南京市蔬菜科学研究所 | A kind of method of Chinese cabbage kind strain cultivation |
| CN109197513B (en) * | 2018-10-19 | 2021-03-30 | 南京市蔬菜科学研究所 | Method for cultivating non-heading Chinese cabbage seed |
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| CN107743837B (en) | 2020-05-12 |
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Granted publication date: 20200512 |