CN107581121A - A kind of prawn culturing method using ozone combination microbial technique - Google Patents
A kind of prawn culturing method using ozone combination microbial technique Download PDFInfo
- Publication number
- CN107581121A CN107581121A CN201710821062.2A CN201710821062A CN107581121A CN 107581121 A CN107581121 A CN 107581121A CN 201710821062 A CN201710821062 A CN 201710821062A CN 107581121 A CN107581121 A CN 107581121A
- Authority
- CN
- China
- Prior art keywords
- water
- ozone
- pond
- days
- seedling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 title claims abstract description 41
- 241000238557 Decapoda Species 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000012258 culturing Methods 0.000 title claims abstract description 12
- 230000000813 microbial effect Effects 0.000 title claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 77
- 241000894006 Bacteria Species 0.000 claims abstract description 21
- 238000009395 breeding Methods 0.000 claims abstract description 13
- 230000001488 breeding effect Effects 0.000 claims abstract description 13
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 9
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 8
- 244000005700 microbiome Species 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 10
- 238000009472 formulation Methods 0.000 claims description 7
- 238000009360 aquaculture Methods 0.000 claims description 6
- 239000004576 sand Substances 0.000 claims description 6
- 244000144974 aquaculture Species 0.000 claims description 5
- 235000013305 food Nutrition 0.000 claims description 4
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 3
- ZKQDCIXGCQPQNV-UHFFFAOYSA-N Calcium hypochlorite Chemical compound [Ca+2].Cl[O-].Cl[O-] ZKQDCIXGCQPQNV-UHFFFAOYSA-N 0.000 claims description 3
- 238000005276 aerator Methods 0.000 claims description 3
- 239000007844 bleaching agent Substances 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000003643 water by type Substances 0.000 claims description 3
- 241000195493 Cryptophyta Species 0.000 abstract description 8
- 230000001954 sterilising effect Effects 0.000 abstract description 6
- 241000192700 Cyanobacteria Species 0.000 abstract description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 4
- 201000010099 disease Diseases 0.000 abstract description 3
- 239000006041 probiotic Substances 0.000 abstract description 3
- 235000018291 probiotics Nutrition 0.000 abstract description 3
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 abstract description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 2
- 229910000037 hydrogen sulfide Inorganic materials 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 150000007524 organic acids Chemical class 0.000 abstract description 2
- 230000001590 oxidative effect Effects 0.000 abstract description 2
- 125000004430 oxygen atom Chemical group O* 0.000 abstract description 2
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000899885 Desmidiales Species 0.000 description 1
- 241001491711 Melosira Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000108664 Nitrobacteria Species 0.000 description 1
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- 241000607598 Vibrio Species 0.000 description 1
- 206010047400 Vibrio infections Diseases 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 230000021332 multicellular organism growth Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Treatment Of Water By Oxidation Or Reduction (AREA)
- Farming Of Fish And Shellfish (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a kind of prawn culturing method using ozone combination microbial technique, removed the evil using ozone as sterilization material, oxygen atom with strong oxidizing property can reach the purpose of sterilizing, afterwards efficient probiotics is launched in the breeding water body after ozone processing, on the one hand later stage can eliminate ammonia nitrogen in breeding water body by discontinuity photosynthetic bacteria of splashing, the harmful substance such as hydrogen sulfide and organic acid, improve water body, on the other hand the breeding of blue-green algae can be suppressed, and green alga, diatom has but obtained effective breeding, water colour is in pale green, the kilters such as yellowish-brown, positive role is served to pond algae structure regulating, it compensate for the influence that the processing of single use ozone is brought to aqueous bio, add aqueous bio vigor and shrimp seedling resistance against diseases.
Description
Technical field
The invention belongs to technical field of aquaculture, and in particular to a kind of prawn using ozone combination microbial technique is supported
Grow method.
Background technology
China is prawn producing country maximum in the world, and the occurrence degree of disease of prawn has what is constantly strengthened to become in recent years
Gesture, shrimp culture industry face bottleneck.In prawn culturing water body, planktonic algae is the main body bait in shrimp ponds, its species, density
Maintenance to Shrimp pond ecosystem plays a crucial role, and there is extremely close relation between being grown with prawn.How to maintain
Good water colour, the excellent planktonic microalgae of culture, the generation that reduces vibrios disease are always to receive much concern during prawn culturing
One of subject matter.
Ozone has very strong sterilizing, taste removal, discoloration, the characteristic of degradation of organic substances as a kind of strong oxidizer, and will not
Residual is produced, is had been widely used in sea, freshwater aquiculture and nursery production.Research shows, breeding water, energy are handled with ozone
Effectively suppress the pathogenic microorganism in the cultivation of the aquatic animals such as fish, Shrimp waste, shellfish, remove harmful bacteria, debirs, oxygen
Change nitrite nitrogen and ammoniacal nitrogen.
Although the effect that ozone is handled breeding water is preferable, ozone has also been eliminated all in water while sterilization
Food organisms, such as nitrobacteria, algae beneficial organism.
The content of the invention
For overcome the deficiencies in the prior art, the present invention provides a kind of prawn culturing using ozone combination microbial technique
Method, using Ozone Water cultured prawn, probiotics is launched with reference to reasonable, promotes beneficial microbe and algae in breeding water body
Growth, increase aqueous bio vigor and shrimp seedling resistance against diseases.
A kind of prawn culturing method using ozone combination microbial technique, including step in detail below：
（1）Pond disinfection：
Put before seedling 20-30 days, thorough disinfection is carried out to band water pond bottom and surrounding with bleaching powder, dosage presses 20g/m3 Concentration
Full pool spilling head, drain within second day water, exposure 7 days；
（2）Pond culture food organisms and rich water：
Ozone Water is used as pond waters, starts to intake putting seedling the last week, can first intake after 40-50cm, use biological rich water
Precious 75kg/hm2, slowly water inlet adds biological rich water treasured to water level 80-120cm, and according to water colour and transparency afterwards；
（3）Apply microorganism formulation：
Putting 2-6 days of before seedling, high-efficiency microorganism preparation of uniformly being splashed into pond, viable bacteria concentration it is stable every milligram 2,000,000,000 with
On, dosage stirs water 2-3 hours splashing while opening aerator, is uniformly distributed strain in 1.0-2.0mg/L；
（4）Seedling is selected to put in a suitable place to breed：
The health young shrimp that body long size is 0.8-1cm is chosen to be put into culture pond, when putting seedling, 22-26 DEG C of water temperature, pH 7.5-
8.5, breeding density is ten thousand tails of 2-4/mu；
（5）Aquaculture management：
A pond water was handled using ozone generator per 4-7 days, per the photosynthetic bacteria for adding 10ppm concentration for 2-4 days, at ozone
Reason is launched to stagger one day and launched with bacterium, while controls water pH value in 7.6-8.5；Change water once daily, add ozone concentration
The water body after 0.2mg/L processing, quantity of exchanged water is the 10-20% in former pond, and every blowdown in 2 days is once；
（6）Feedstuff feeding：
Shrimp seedling starts to feed feed after launching 3-6 days, from 6 points to 21 points, is fed once per 3-4 hours, feeding after afternoon
Amount accounts for the 50-60% of whole day feeding volume.
Wherein, the step（2）In by rich water make water present yellow green, light brown, brown, 30-40 lis of transparency
Meter Wei Yi.
Wherein, the step（2）Pond water water source come from beach sand filter phreatic water, through precipitation, sand filter, ozone machine
After be re-introduced into culture pond, the content of ozone in water is 0.2-0.5mg/L.
Wherein, the step（3）Middle microorganism formulation refers to photosynthetic bacteria, bacillus amyloliquefaciens, denitrifying bacteria
Mix bacterium agent.
Compared with prior art, the present invention use ozone to be removed the evil material as sterilization, securely and reliably, efficient, ozone decomposed
Into the oxygen atom with strong oxidizing property can reach the purpose of sterilizing, launched afterwards in the breeding water body after ozone processing efficient
Probiotics, early stage blue-green algae account for main advantage, middle and later periods green alga turns into sociales, and blue-green algae is referred to as having in aquaculture
Evil algae, therefore carries out shrimp seedling after microorganism formulation is launched and puts in a suitable place to breed again in a few days, now exist in water body substantial amounts of boat-shaped algae,
Melosira, crisp bar algae etc. and green alga and desmids with flagellum, can directly as the high-quality bait of shrimp seedling, water stabilization, after
On the one hand phase can eliminate the nuisances such as ammonia nitrogen, hydrogen sulfide and organic acid in breeding water body by discontinuity photosynthetic bacteria of splashing
Matter, improve water body, on the other hand stabilizing water quality can suppress the breeding of blue-green algae, and green alga, diatom obtained it is effective numerous
Grow, water colour serves positive role to pond algae structure regulating, compensate for single make in kilters such as pale green, yellowish-brown
The influence brought to aqueous bio is handled with ozone.
Embodiment
A kind of prawn culturing method using ozone combination microbial technique, including step in detail below：
（1）Pond disinfection：
Put before seedling 30 days, thorough disinfection is carried out to band water pond bottom and surrounding with bleaching powder, dosage presses 20g/m3 The full pond of concentration
Splash, drain within second day water, exposure 7 days；
（2）Pond culture food organisms and rich water：
Ozone Water is used as pond waters, starts to intake putting seedling the last week, after the 50cm that can first intake, uses biological rich water precious
75kg/hm2, slowly water inlet adds biological rich water treasured to water level 120cm, and according to water colour and transparency afterwards, and water is presented
Yellow green, light brown, brown, transparency is in 35cm；
（3）Apply microorganism formulation：
To put 5 days of before seedling, high-efficiency microorganism preparation of uniformly being splashed into pond, viable bacteria concentration is stable at every milligram more than 2,000,000,000,
Dosage stirs water 2 hours splashing while opening aerator, is uniformly distributed strain in 1.5mg/L；
（4）Seedling is selected to put in a suitable place to breed：
The health young shrimp that body long size is 0.8-1cm is chosen to be put into culture pond, when putting seedling, 25 DEG C of water temperature, pH 7.5-
8.5, breeding density is 40,000 tails/mu；
（5）Aquaculture management：
Every 5 days using ozone generator handle a pond water, every 3 days photosynthetic bacterias for adding 10ppm concentration, ozone processing with
Bacterium is launched to stagger one day and launched, while controls water pH value in 7.6-8.5；Change water once daily, add ozone concentration and exist
Water body after 0.2mg/L processing, quantity of exchanged water is the 20% of former pond, and every blowdown in 2 days is once；
（6）Feedstuff feeding：
Shrimp seedling starts to feed feed after launching 4 days, from 6 points to 21 points, feeds once within every 3 hours, the feeding volume after afternoon accounts for
The 60% of whole day feeding volume.
Wherein, the step（2）Pond water water source come from beach sand filter phreatic water, through precipitation, sand filter, ozone machine
After be re-introduced into culture pond, the content of ozone in water is 0.3mg/L.
Wherein, the step（3）Middle microorganism formulation refers to photosynthetic bacteria, bacillus amyloliquefaciens, denitrifying bacteria
Mix bacterium agent.
Claims (4)
- A kind of 1. prawn culturing method using ozone combination microbial technique, it is characterised in that including step in detail below：（1）Pond disinfection：Put before seedling 20-30 days, thorough disinfection is carried out to band water pond bottom and surrounding with bleaching powder, dosage presses 20g/m3 Concentration is complete Pond is splashed, and drains within second day water, exposure 7 days；（2）Pond culture food organisms and rich water：Ozone Water is used as pond waters, starts to intake putting seedling the last week, can first intake after 40-50cm, use biological rich water Precious 75kg/hm2, slowly water inlet adds biological rich water treasured to water level 80-120cm, and according to water colour and transparency afterwards；（3）Apply microorganism formulation：Putting 2-6 days of before seedling, high-efficiency microorganism preparation of uniformly being splashed into pond, viable bacteria concentration it is stable every milligram 2,000,000,000 with On, dosage stirs water 2-3 hours splashing while opening aerator, is uniformly distributed strain in 1.0-2.0mg/L；（4）Seedling is selected to put in a suitable place to breed：The health young shrimp that body long size is 0.8-1cm is chosen to be put into culture pond, when putting seedling, 22-26 DEG C of water temperature, pH 7.5- 8.5, breeding density is ten thousand tails of 2-4/mu；（5）Aquaculture management：A pond water was handled using ozone generator per 4-7 days, per the photosynthetic bacteria for adding 10ppm concentration for 2-4 days, at ozone Reason is launched to stagger one day and launched with bacterium, while controls water pH value in 7.6-8.5；Change water once daily, add ozone concentration The water body after 0.2mg/L processing, quantity of exchanged water is the 10-20% in former pond, and every blowdown in 2 days is once；（6）Feedstuff feeding：Shrimp seedling starts to feed feed after launching 3-6 days, from 6 points to 21 points, is fed once per 3-4 hours, feeding after afternoon Amount accounts for the 50-60% of whole day feeding volume.
- 2. a kind of prawn culturing method using ozone combination microbial technique according to claims 1, its feature exist In the step（2）In by rich water make water that yellow green, light brown, brown be presented, 30-40 centimetres of transparency is advisable.
- 3. a kind of prawn culturing method using ozone combination microbial technique according to claims 1, its feature exist In the step（2）Pond water water source come from beach sand filter phreatic water, be re-introduced into after precipitation, sand filter, ozone machine foster Grow in the pool, the content of ozone in water is 0.2-0.5mg/L.
- 4. a kind of prawn culturing method using ozone combination microbial technique according to claims 1, its feature exist In the step（3）Middle microorganism formulation refers to the mix bacterium agent of photosynthetic bacteria, bacillus amyloliquefaciens, denitrifying bacteria.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710821062.2A CN107581121B (en) | 2017-09-13 | 2017-09-13 | Prawn culture method using ozone and microbial technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710821062.2A CN107581121B (en) | 2017-09-13 | 2017-09-13 | Prawn culture method using ozone and microbial technology |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107581121A true CN107581121A (en) | 2018-01-16 |
| CN107581121B CN107581121B (en) | 2020-09-29 |
Family
ID=61051265
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710821062.2A Expired - Fee Related CN107581121B (en) | 2017-09-13 | 2017-09-13 | Prawn culture method using ozone and microbial technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107581121B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111747617A (en) * | 2020-07-21 | 2020-10-09 | 中国水产科学研究院淡水渔业研究中心 | Method for treating pond blue algae bloom |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101647415A (en) * | 2009-09-08 | 2010-02-17 | 中国水产科学研究院淡水渔业研究中心 | Method for culturing ripe Luo pond crayfishes |
| CN103843703A (en) * | 2013-12-30 | 2014-06-11 | 浙江省海洋开发研究院 | Penaeus vannamei boone low-salinity culturing method |
| CN103843707A (en) * | 2013-12-30 | 2014-06-11 | 浙江省海洋开发研究院 | Method for breeding penaeus vannamei boone by using ozone water |
| CN104585106A (en) * | 2015-02-11 | 2015-05-06 | 上海海洋大学 | Method for regulating micro-ecology of shrimp culture pond by utilizing photobioreactor |
| CN104642221A (en) * | 2014-11-12 | 2015-05-27 | 重庆茂升林业开发有限公司 | Penaeus japonicus pond culture technology |
-
2017
- 2017-09-13 CN CN201710821062.2A patent/CN107581121B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101647415A (en) * | 2009-09-08 | 2010-02-17 | 中国水产科学研究院淡水渔业研究中心 | Method for culturing ripe Luo pond crayfishes |
| CN103843703A (en) * | 2013-12-30 | 2014-06-11 | 浙江省海洋开发研究院 | Penaeus vannamei boone low-salinity culturing method |
| CN103843707A (en) * | 2013-12-30 | 2014-06-11 | 浙江省海洋开发研究院 | Method for breeding penaeus vannamei boone by using ozone water |
| CN104642221A (en) * | 2014-11-12 | 2015-05-27 | 重庆茂升林业开发有限公司 | Penaeus japonicus pond culture technology |
| CN104585106A (en) * | 2015-02-11 | 2015-05-06 | 上海海洋大学 | Method for regulating micro-ecology of shrimp culture pond by utilizing photobioreactor |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111747617A (en) * | 2020-07-21 | 2020-10-09 | 中国水产科学研究院淡水渔业研究中心 | Method for treating pond blue algae bloom |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107581121B (en) | 2020-09-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110122386A (en) | A method of being suitble to high salinity mariculture prawn | |
| CN107581122A (en) | It is a kind of to carry out fresh water aquaculture method for penaeus vannamei using biological flocculation technology | |
| CN108913632A (en) | A kind of compound microorganism ferments liquid used for aquiculture and its preparation method and application | |
| CN105130012A (en) | Water quality improver | |
| CN104137797B (en) | The fresh water ecological cultivation method of Penaeus Vannmei | |
| CN1846481A (en) | Mandarin cultivating method in pond | |
| CN104585105B (en) | Water ecology formula method for culturing seedlings outside Litopenaeus vannamei room | |
| CN110628644B (en) | Novel biological floc, application thereof and method for marking crude litopenaeus vannamei by using same | |
| CN108633792B (en) | Method for comprehensive planting and breeding of fishery-agriculture in saline-alkali soil | |
| CN109122443A (en) | Australia freshwater lobster recirculated water oogenesis hatching system and method for culturing seedlings | |
| CN111771772B (en) | Grouper fry breeding method | |
| CN103444603A (en) | Method for culturing grouper fries in small water body | |
| CN110663595B (en) | Tilapia breeding method | |
| CN104651282B (en) | A kind of preparation method of Composite Photosynthetic Bacteria preparation | |
| CN103641229B (en) | Method for regulating and controlling of water quality of Litopenaeus Vannamei culture pond | |
| CN107251859A (en) | A kind of preparation of multi-functional microbial inoculum compound of promotion shrimp crab shell and application process | |
| CN107926794A (en) | A kind of high-efficiency breeding method of Macrobrachium rosenbergii | |
| TW201630530A (en) | Smart aquarium | |
| CN107581121A (en) | A kind of prawn culturing method using ozone combination microbial technique | |
| CN108770742A (en) | A kind of method for culturing seedlings of Macrobrachium rosenbergii high-survival rate | |
| CN108633802A (en) | A kind of method of biological breeding Penaeus Vannmei parent shrimp | |
| CN109588350A (en) | A kind of Penaeus Vannmei batch production desalination cyclic culture method | |
| CN111285542B (en) | Treatment process of industrial seedling raising tail water of stichopus japonicus for cultivating unicellular algae | |
| CN107306861A (en) | A kind of biological feed feeds the cultural method of fish seedling | |
| CN108782385A (en) | A kind of cultural method of Macrobrachium rosenbergii seedling |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200929 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |