CN107494284A - A kind of seed selection of fleece-flower root and cultural method - Google Patents

A kind of seed selection of fleece-flower root and cultural method Download PDF

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Publication number
CN107494284A
CN107494284A CN201710975333.XA CN201710975333A CN107494284A CN 107494284 A CN107494284 A CN 107494284A CN 201710975333 A CN201710975333 A CN 201710975333A CN 107494284 A CN107494284 A CN 107494284A
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fleece
flower root
cultural method
seed selection
seedling
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CN107494284B (en
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梁木强
张晓莉
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Deqin County Planting Professional Cooperatives From Kant
Guangdong Yin Tian Agricultural Science And Technology Co Ltd
Guangdong Dekang Agriculture Technology Co Ltd
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Deqin County Planting Professional Cooperatives From Kant
Guangdong Yin Tian Agricultural Science And Technology Co Ltd
Guangdong Dekang Agriculture Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008Methods for regeneration to complete plants

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  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Cultivation Of Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of seed selection of fleece-flower root and cultural method, including selection of land site preparation, screening high-quality root tuber, vernalization processing, tissue cultures, the induction of magnetoelectricity field and transplanting.Seed selection and cultural method the screening high-quality root tuber from wild fleece-flower root introduces a collection of the fleece-flower root of the present invention, and the stem apex that obtains through vernalization processing obtains nutritive cup seedlings by tissue culture, ensure that the superiority of follow-up fleece-flower root seedling;Another to cause fleece-flower root seedling to strengthen diseases and insect pests resistance using magnetoelectricity field co-induction, well developed root system, root tuber growing way are healthy and strong, improve water, the ability of fertilizer of absorbing, transplanting survival rate is up to 99%, so as to reach the purpose of fleece-flower root volume increase.

Description

A kind of seed selection of fleece-flower root and cultural method
Technical field
The present invention relates to technical field of Chinese herbal medicine cultivation is defended, in particular to a kind of seed selection of the fleece-flower root and cultivation side Method.
Background technology
The fleece-flower root is the perennial winding liana of polygonaceae fleece-flower root category fleece-flower root kind, is used as medicine with root tuber, can calm the nerves, support Blood, active, removing toxic substances (preventing malaria), disappear carbuncle;RADIX POLYGONI MULTIFLORI PREPARATA can help essence and blood, black beard and hair, strengthening the bones and muscles, filling liver kidney, be your common detail medicine Material.Fleece-flower root each province and city on the south China the Yellow River are distributed, polygonum multiflorum planting nearly millennium history,《Bencao Tujing》(Song Su Song writings in 1020~1101):" fleece-flower root, the present have it at place.Spring gives birth to seedling leaf, and leaf is relative if sweet potato is without gloss.Its stem Between sprawling bamboo and wood wall.Knot has that rib is tiny like buckwheat, just as grain is big.Autumn and winter take root, big person such as fist, respectively have five rib valves, seemingly Small muskmelon.”
It is optimal with wild fleece-flower root kind in fleece-flower root industry, but predatory excavation is wild how to appear for the first time as serious, makes Fleece-flower root wild resource faces deficient crisis.Long-term use of vegetative manner in cultivation simultaneously, most commonly with not being inconsistent The defect ware for closing sales terms selects the excellent breeding of excellent training as potato kind or the use tendril cuttage seeding of non-selectivity, this shortage Mode, result in variety deterioration, survival rate, quality, yield, root tuber diseases and insect pests resistance drastically decline, thus also indirectly orderization The usage amount of fertilizer and agricultural chemicals increases further, not only allows the input cost of peasant household to increase, and causes agricultural pollution increasing, agricultural chemicals Residual is serious, and the medicinal medical effect and edible safety that have impact on the fleece-flower root are horizontal.In addition, needed in fleece-flower root general planting Support is climbed up by holding on to using substantial amounts of bamboo pole as tendril, during which also needs to multiple artificial bud picking, cost is higher.
The content of the invention
In view of this, a kind of seed selection of fleece-flower root provided by the invention and cultural method, preferably overcome above-mentioned existing The problem of technology is present and defect, the screening high-quality root tuber from wild fleece-flower root introduces a collection, and handled through vernalization, take its stem apex to pass through Tissue culture obtains nutritive cup seedlings, ensure that the superiority of fleece-flower root seedling;It is another make it that seedling enhancing is anti-using magnetoelectricity field co-induction Pest and disease damage ability, well developed root system, root tuber growing way are healthy and strong, improve water, the ability of fertilizer of absorbing, transplanting survival rate up to 99%, so as to Reach the purpose of fleece-flower root volume increase.
A kind of seed selection of fleece-flower root and cultural method, the cultural method comprise the following steps:
(1) root tuber is screened from the wild fleece-flower root:For its Growing years more than 3 years, effective ingredient was higher than NF mark Standard, head are in well-balanced spindle, and surface is in rufous or bronzing, and disease-free spot, section is in bright yellow and with obvious gold Yellow Nanjing brocade decorative pattern;
(2) vernalization processing is carried out to the root tuber, it is grown stem eye;
(3) stem apex of the root tuber is taken, by tissue cultures, to obtain nutritive cup seedlings as explant;
(4) nutritive cup seedlings are cultivated under greenhouse-environment, will when the nutritive cup seedlings grow into 30~40cm height Nutritive cup seedlings are cut into the band bud cutting fringe of 10~15cm length, carry out cuttage, then handle the progress magnetoelectricity field induction of cuttage bar To seedling;
(5) when the seedling early growth is to 10~15cm height, be transplanted to plantation field in, pour it is permeable, keep soil it is wet Profit.
Further, in step (1), the length of the root tuber is 6~15cm, a diameter of 6~10cm.
Further, in step (2), the vernalization processing procedure includes:The root tuber is planted in the sand by sterilization In son, it is then placed within incubator and carries out vernalization, the temperature in the incubator is 23~27 DEG C.
Further, in step (4), in the magnetoelectricity field induction processing procedure:Voltage is 70~90kv, magnetic induction intensity For 1500~1700GS;The magnetoelectricity field induction processing time is 10~20min.
Further, base manure has been applied in the plantation field, and the base manure is the mixed fertilizer of barnyard manure and plant ash;Every mu of application The amount of the base manure is 1500~3500kg.
Further, the width of the furrow in the plantation field is 70~100cm, is highly 25~30cm.
Further, covering with ground sheeting is carried out on the furrow in the plantation field.
Further, the width of the mulch film is 1.2~1.5m.
Further, in step (5), after transplanting, the plant of seedling is away from for 25~40cm, line-spacing in the plantation field For 35~50cm.
Further, also include after the step (5):When the seedling grows to 25~50cm, strong 1 is left ~2 Tengs are climing, erase remaining Teng is climing or cuts, will be thin and delicate then after the furrow face in the full plantation of the climing covering of Teng Teng is climing to be removed.
Compared with prior art, the seed selection of a kind of fleece-flower root of the invention and the beneficial effect of cultural method are:
(1) seed selection and cultural method the screening high-quality root tuber from wild fleece-flower root introduces a collection of the fleece-flower root of the invention, and pass through The stem apex that obtains of vernalization processing obtains nutritive cup seedlings by tissue culture, ensure that the superiority of follow-up fleece-flower root seedling;Separately use magnetic Electric field co-induction causes fleece-flower root seedling to strengthen diseases and insect pests resistance, and well developed root system, root tuber growing way are healthy and strong, raising absorption water, The ability of fertilizer, transplanting survival rate is up to 99%, so as to reach the purpose of fleece-flower root volume increase.
(2) seed selection of the fleece-flower root of the invention and cultural method use covering with ground sheeting on the furrow in plantation field, effectively Reach water conservation, fertilizer conservation, the effect increase soil fertility, reduce the expense of subsequent artefacts' weeding and artificial bud picking, effectively reduce The cultivation cost of the fleece-flower root.
To enable the above objects, features and advantages of the present invention to become apparent, preferred embodiment cited below particularly, make detailed It is described as follows.
Embodiment
For the ease of understanding the present invention, technical scheme is elaborated with reference to the mode of embodiment, Many details are elaborated in the following description in order to fully understand the present invention.
But the invention can be embodied in many other ways as described herein, those skilled in the art can be with Similar improvement is done in the case of without prejudice to intension of the present invention, therefore the present invention is not limited to the specific embodiments disclosed below.
Unless otherwise defined, all technologies used herein and scientific terminology have and the common skill of art of the present invention The identical implication that art personnel are generally understood that.When contradiction be present, the definition in this specification is defined.
Term as used herein:
Term "comprising" used herein, " comprising ", " having ", " containing " or its any other deformation, it is intended that covering Non-exclusionism includes.Wanted for example, composition, step, method, product or device comprising listed elements are not necessarily limited to those Element, but can include not expressly listed other key elements or such a composition, step, method, product or device it is intrinsic Key element.
Conjunction " by ... form " exclude any key element do not pointed out, step or component.If be used in claim, This phrase will make claim be closed, it is not included the material in addition to the material of those descriptions, but relative Except customary impurities.When phrase " by ... form " be rather than immediately following theme in the clause that appears in claim main body after When, it is only limited to the key element described in the clause;Other key elements be not excluded as entirety the claim it Outside.
Equivalent, concentration or other values or parameter are excellent with scope, preferred scope or a series of upper limit preferred values and lower limit During the Range Representation that choosing value limits, this, which is appreciated that, specifically discloses by any range limit or preferred value and any scope All scopes that any pairing of lower limit or preferred value is formed, regardless of whether the scope separately discloses.For example, when open Scope " when 1~5 ", described scope should be interpreted as including scope " 1~4 ", " 1~3 ", " 1~2 ", " 1~2 and 4~ 5 ", " 1~3 and 5 " etc..When number range is described herein, unless otherwise indicated, otherwise the scope is intended to include its end Value and all integers and fraction within the range.
"and/or" is used to represent that one of illustrated situation or both may to occur, for example, A and/or B includes (A And B) and (A or B).
A kind of seed selection of fleece-flower root and cultural method, the cultural method are further comprising the steps of:
(1) from wild fleece-flower root kind screening high-quality root tuber:For Growing years more than 3 years, effective ingredient was higher than NF mark Standard, head are in well-balanced spindle, and surface is in rufous or bronzing, disease-free spot, and section is in bright yellow and with obvious gold Yellow Nanjing brocade decorative pattern.In addition, the preferred flexibility of high-quality root tuber is small, surface longitudinal furrow is uniform, wrinkle is fine and close, and sense of touch is hard, no Frangibility, and there is strong fragrance, mildly bitter flavor and it is sweet puckery.Preferably, the high-quality root tuber is selected from Zhaoqing City of Guangdong Province Deqing The local wild polygonum multiforum root tuber in county.Certainly, the present invention is not intended to limit the source place of high-quality root tuber.
(2) vernalization processing is carried out to the high-quality root tuber, it is grown stem eye.
(3) stem apex of the high-quality root tuber is taken, by tissue cultures, to obtain nutritive cup seedlings as explant.
(4) nutritive cup seedlings are cultivated, when the nutritive cup seedlings grow into 30~40cm such as 30cm, 32cm, 35cm, 38cm Or 40cm etc. height when, nutritive cup seedlings are cut into 10~15cm such as 10cm, 11cm, 12cm, 13cm, 14cm or 15cm equal lengths Band bud cutting fringe, carry out cuttage, then by cuttage bar progress magnetoelectricity field induction handle to obtain seedling.Wherein, preferred growth it is good, Strong, healthy nutritive cup seedlings, it is cut into band bud cutting fringe.
(5) when the seedling early growth to 10~15cm such as 10cm, 11cm, 12cm, 13cm, 14cm or 15cm height, It is transplanted in plantation field, pours permeable, holding ground moistening.
It should be noted that plantation field selection soil layer it is deep, it is loose it is fertile, rich in humus and have stable cleaning The gentle hillside fields of river water supply applies base manure as plantation field, then makes furrow.
Preferably, the base manure is the mixed fertilizer of barnyard manure and plant ash;Every mu of amount for applying the base manure for 1500~ 3500kg such as 1500kg, 2000kg, 2500kg, 3000kg or 3500kg etc..
Preferably, it is described plantation field furrow width for 70~100cm such as 70cm, 75cm, 80cm, 85cm, 90cm, 95cm or 100cm etc. is highly 25~30cm as such as 25cm, 26cm, 27cm, 28cm, 29cm or 30cm.
Preferably, covering with ground sheeting is carried out on the furrow in the plantation field.
Mulch film is used it should be noted that not had been reported that in existing cultivation technology of polygonum multiflorum containing, but seedling is directly moved Plant and plant in field, easy long weeds, and easily water, fertilizer are lost in, and subsequently need to expend a large amount of manpowers progress artificial bud pickings.
Covering with ground sheeting have heat, water conservation, fertilizer conservation, improve soil physico-chemical property, increase soil fertility, suppress weeds life It is long, mitigate the effect of disease, also have the function of reducing humidity in the case of continuous rainfall, so as to promote root tuber to grow, Yield positive results ahead of time, increase yield, reduce the effect such as labor cost.The present invention is further by using covering with ground sheeting in plot of land On, water conservation, fertilizer conservation are effectively achieved, the effect increase soil fertility, reduces the expense of subsequent artefacts' weeding and artificial bud picking, So as to effectively reduce the cultivation cost of the fleece-flower root.
Preferably, the width of the mulch film is 1.2~1.5m such as 1.2cm, 1.3cm, 1.4cm or 1.5cm.
Preferably, in step (1), the length of the high-quality root tuber is 6~15cm such as 6cm, 8cm, 10cm, 12cm, 14cm Or 15cm, a diameter of 6~10cm such as 6cm, 7cm, 8cm, 9cm or 15cm.
Preferably, in step (2), the vernalization processing procedure includes:The high-quality root tuber is planted in by sterilization In sand, it is then placed within incubator and carries out vernalization.
Temperature in the incubator is 23~27 DEG C such as 23 DEG C, 24 DEG C, 25 DEG C, 26 DEG C or 27 DEG C.
Preferably, in step (4), in the magnetoelectricity field induction processing procedure:Voltage be 70~90kv such as 70kv, 75kv, 80kv, 85kv or 90kv etc., the magnetoelectricity field induction processing time is 10~20min.
Preferably, in step (5), after transplanting, it is described plantation field in seedling plant away from for 25~40cm such as 25cm, 28cm, 30cm, 32cm, 35cm, 38cm or 40cm etc., line-spacing be 35~50cm such as 35cm, 38cm, 40cm, 42cm, 45cm, 48cm or 50cm etc..
Preferably, also include after the step (5):When the seedling grow to 25~50cm such as 25cm, 30cm, When 35cm, 40cm, 45cm or 50cm etc., it is climing to leave 1~2 strong Teng, erases remaining Teng is climing or cuts, Ran Hou After the furrow face in the climing full plantation of covering of Teng, remove thin and delicate Teng is climing.
Seed selection and cultural method the screening high-quality root tuber from wild fleece-flower root introduces a collection of the fleece-flower root of the present invention, and through vernalization The stem apex that obtains of processing obtains nutritive cup seedlings by tissue culture, ensure that the superiority of follow-up fleece-flower root seedling;Separately use magnetoelectricity field Co-induction causes fleece-flower root seedling to strengthen diseases and insect pests resistance, well developed root system, root tuber growing way are healthy and strong, improves and absorbs water, fertilizer Ability, transplanting survival rate is up to 99%, so as to reach the purpose of fleece-flower root volume increase.
For the ease of understanding the present invention, technical scheme is further illustrated with reference to embodiment.Applicant Statement, the present invention illustrate the detailed process equipment of the present invention and technological process, but not office of the invention by following embodiments It is limited to following detailed process equipments and technological process, that is, does not mean that the present invention should rely on following detailed process equipment and technique streams Cheng Caineng is implemented.Person of ordinary skill in the field is it will be clearly understood that any improvement in the present invention, to each original of product of the present invention The equivalence replacement of material and the addition of auxiliary element, the selection of concrete mode etc., all fall within protection scope of the present invention and open model Within enclosing.
Embodiment 1
A kind of seed selection of fleece-flower root and cultural method, comprise the following steps:
(1) the screening high-quality root tuber from the local wild fleece-flower root in Zhaoqing City of Guangdong Province Deqing County:Root tuber Growing years surpass 3 years are spent, effective ingredient is higher than NF standard, and head is in well-balanced spindle, and flexibility is small, length 7cm, a diameter of 6cm;Surface is in rufous or bronzing, longitudinal furrow are uniform, wrinkle is fine and close, disease-free spot, and sense of touch is hard, is not easily broken, and section is in Bright yellow simultaneously carries obvious golden yellow Nanjing brocade decorative pattern;With strong fragrance, mildly bitter flavor and it is sweet puckery.
(2) the high-quality root tuber obtained to above-mentioned screening carries out vernalization processing:The high-quality root tuber of the fleece-flower root is planted in by disappearing In the sand of poison, it is in 23 DEG C of incubators to place temperature, it is grown stem eye.
(3) stem apex of the high-quality root tuber is taken, by tissue cultures routine techniques, to obtain nutritive cup seedlings as explant.
(4) nutritive cup seedlings are placed under the conditions of greenhouse-environment and cultivated, when the nutritive cup seedlings grow into 30cm height, Good, strong, the healthy nutritive cup seedlings of growth selection are cut into the band bud cutting fringe of 10cm length, cuttage are carried out, then to cuttage bar Magnetoelectricity field induction is carried out to handle to obtain seedling;Wherein, in magnetoelectricity field induction processing procedure:Voltage is 70kv, and magnetic induction intensity is 1500GS;Magnetoelectricity field induction processing time is 10min.
(5) select away from urban district and industrial area, around pollution-free source, the transitable gentle hillside fields of vehicle as plantation Ground, the plantation field plot soil layer is deep, loose fertile, rich in humus, there is stable healthy stream water supply;Every mu of basis Actual soil fertility applies barnyard manure, plant ash mixed fertilizer 1500kg makees base manure, is raked the soil level after fertilising 1 time, fertilizer is mixed with table soil Uniformly;Then width is made to be 70cm, be highly 25cm plot of land, then in the mulch film that cover width above is 1.2m.
(6) when the height of seedling early growth to 10cm, cloudy day or fine day at dusk by seedling transplanting to plant field in, Make the plant of seedling in plantation field away from for 25cm, line-spacing 35cm;Then to the seedling watering after transplanting, and soil is kept Moistening.
(7) when seedling grows to 25cm, it is climing to leave 2 strong Tengs, erases remaining Teng is climing or cuts, Ran Hou After the furrow face in the climing full plantation of covering of Teng, appropriate trimming is carried out according to the growing state of tendril, is removed thin and delicate Teng is climing.
(8) after occurring bud on tendril, bud is removed.
Embodiment 2
A kind of seed selection of fleece-flower root and cultural method, comprise the following steps:
(1) the screening high-quality root tuber from the local wild fleece-flower root in Zhaoqing City of Guangdong Province Deqing County:Root tuber Growing years surpass 3 years are spent, effective ingredient is higher than NF standard, and head is in well-balanced spindle, and flexibility is small, length 10cm, a diameter of 8cm;Surface is in rufous or bronzing, longitudinal furrow are uniform, wrinkle is fine and close, disease-free spot, and sense of touch is hard, is not easily broken, and section is in Bright yellow simultaneously carries obvious golden yellow Nanjing brocade decorative pattern;With strong fragrance, mildly bitter flavor and it is sweet puckery.
(2) the high-quality root tuber obtained to above-mentioned screening carries out vernalization processing:The high-quality root tuber of the fleece-flower root is planted in by disappearing In the sand of poison, it is in 25 DEG C of incubators to place temperature, it is grown stem eye.
(3) stem apex of the high-quality root tuber is taken, by tissue cultures routine techniques, to obtain nutritive cup seedlings as explant.
(4) nutritive cup seedlings are placed under the conditions of greenhouse-environment and cultivated, when the nutritive cup seedlings grow into 30cm height, Good, strong, the healthy nutritive cup seedlings of growth selection are cut into the band bud cutting fringe of 12cm length, cuttage are carried out, then to cuttage bar Magnetoelectricity field induction is carried out to handle to obtain seedling;Wherein, in magnetoelectricity field induction processing procedure:Voltage is 80kv, and magnetic induction intensity is 1600GS;Magnetoelectricity field induction processing time is 15min.
(5) select away from urban district and industrial area, around pollution-free source, the transitable gentle hillside fields of vehicle as plantation Ground, the plantation field plot soil layer is deep, loose fertile, rich in humus, there is stable healthy stream water supply;Every mu of basis Actual soil fertility applies barnyard manure, plant ash mixed fertilizer 2500kg makees base manure, is raked the soil level after fertilising 1 time, fertilizer is mixed with table soil Uniformly;Then width is made to be 85cm, be highly 28cm plot of land, then in the mulch film that cover width above is 1.3m.
(6) when the height of seedling early growth to 12cm, cloudy day or fine day at dusk by seedling transplanting to plant field in, Make the plant of seedling in plantation field away from for 35cm, line-spacing 40cm;Then to the seedling watering after transplanting, and soil is kept Moistening.
(7) when seedling grows to 35cm, it is climing to leave 1 strong Teng, erases remaining Teng is climing or cuts, Ran Hou After the furrow face in the climing full plantation of covering of Teng, appropriate trimming is carried out according to the growing state of tendril, is removed thin and delicate Teng is climing.
(8) after occurring bud on tendril, bud is removed.
Embodiment 3
A kind of seed selection of fleece-flower root and cultural method, comprise the following steps:
(1) the screening high-quality root tuber from the local wild fleece-flower root in Zhaoqing City of Guangdong Province Deqing County:Root tuber Growing years surpass 3 years are spent, effective ingredient is higher than NF standard, and head is in well-balanced spindle, and flexibility is small, length 15cm, a diameter of 10cm;Surface is in rufous or bronzing, longitudinal furrow are uniform, wrinkle is fine and close, disease-free spot, and sense of touch is hard, is not easily broken, and section is in Bright yellow simultaneously carries obvious golden yellow Nanjing brocade decorative pattern;With strong fragrance, mildly bitter flavor and it is sweet puckery.
(2) the high-quality root tuber obtained to above-mentioned screening carries out vernalization processing:The high-quality root tuber of the fleece-flower root is planted in by disappearing In the sand of poison, it is in 27 DEG C of incubators to place temperature, it is grown stem eye.
(3) stem apex of the high-quality root tuber is taken, by tissue cultures routine techniques, to obtain nutritive cup seedlings as explant.
(4) nutritive cup seedlings are placed under the conditions of greenhouse-environment and cultivated, when the nutritive cup seedlings grow into 30cm height, Good, strong, the healthy nutritive cup seedlings of growth selection are cut into the band bud cutting fringe of 15cm length, cuttage are carried out, then to cuttage bar Magnetoelectricity field induction is carried out to handle to obtain seedling;Wherein, in magnetoelectricity field induction processing procedure:Voltage is 90kv, and magnetic induction intensity is 1700GS;Magnetoelectricity field induction processing time is 20min.
(5) select away from urban district and industrial area, around pollution-free source, the transitable gentle hillside fields of vehicle as plantation Ground, the plantation field plot soil layer is deep, loose fertile, rich in humus, there is stable healthy stream water supply;Every mu of basis Actual soil fertility applies barnyard manure, plant ash mixed fertilizer 3500kg makees base manure, is raked the soil level after fertilising 1 time, fertilizer is mixed with table soil Uniformly;Then width is made to be 30cm, be highly 40cm plot of land, then in the mulch film that cover width above is 1.5m.
(6) when the height of seedling early growth to 15cm, cloudy day or fine day at dusk by seedling transplanting to plant field in, Make the plant of seedling in plantation field away from for 40cm, line-spacing 50cm;Then to the seedling watering after transplanting, and soil is kept Moistening.
(7) when seedling grows to 50cm, it is climing to leave 2 strong Tengs, erases remaining Teng is climing or cuts, Ran Hou After the furrow face in the climing full plantation of covering of Teng, appropriate trimming is carried out according to the growing state of tendril, is removed thin and delicate Teng is climing.
(8) after occurring bud on tendril, bud is removed.
The preferred embodiments of the present invention are above are only, are not intended to limit the invention, for those skilled in the art For member, inventive formulation and preparation technology can have various modifications and variations.Within the spirit and principles of the invention, institute Any modification, equivalent substitution and improvements of work etc., should be included in the scope of the protection.

Claims (10)

1. seed selection and the cultural method of a kind of fleece-flower root, it is characterised in that:Comprise the following steps:
(1) root tuber is screened from the wild fleece-flower root:For its Growing years more than 3 years, effective ingredient was higher than NF standard, individual Head is in well-balanced spindle, and surface is in rufous or bronzing, and disease-free spot, section is in bright yellow and with obvious golden yellow cloud Bright and beautiful decorative pattern;
(2) vernalization processing is carried out to the root tuber, it is grown stem eye;
(3) stem apex of the root tuber is taken, by tissue cultures, to obtain nutritive cup seedlings as explant;
(4) nutritive cup seedlings are cultivated under greenhouse-environment, when the nutritive cup seedlings grow into 30~40cm height, by nutrition Cup seedling is cut into the band bud cutting fringe of 10~15cm length, carries out cuttage, then handles the progress magnetoelectricity field induction of cuttage bar and is planted Seedling;
(5) when the seedling early growth is to 10~15cm height, it is transplanted in plantation field, pours permeable, holding ground moistening.
2. seed selection and the cultural method of the fleece-flower root according to claim 1, it is characterised in that:In step (1), the root tuber Length be 6~15cm, a diameter of 6~10cm.
3. seed selection and the cultural method of the fleece-flower root according to claim 1, it is characterised in that:In step (2), the vernalization Processing procedure includes:The root tuber is planted in the sand by sterilization, is then placed within incubator and carries out vernalization, it is described Temperature in incubator is 23~27 DEG C.
4. seed selection and the cultural method of the fleece-flower root according to claim 1, it is characterised in that:In step (4), the magnetoelectricity In field induction processing procedure:Voltage is 70~90kv, and magnetic induction intensity is 1500~1700GS;During the magnetoelectricity field induction processing Between be 10~20min.
5. seed selection and the cultural method of the fleece-flower root according to claim 1, it is characterised in that:Base is applied in the plantation field Fertilizer, the base manure are the mixed fertilizer of barnyard manure and plant ash;Every mu of amount for applying the base manure is 1500~3500kg.
6. seed selection and the cultural method of the fleece-flower root according to claim 1, it is characterised in that:The furrow in the plantation field Width is 70~100cm, is highly 25~30cm.
7. seed selection and the cultural method of the fleece-flower root according to claim 1, it is characterised in that:On the furrow in the plantation Carry out covering with ground sheeting.
8. seed selection and the cultural method of the fleece-flower root according to claim 7, it is characterised in that:The width of the mulch film is 1.2~1.5m.
9. seed selection and the cultural method of the fleece-flower root according to claim 1, it is characterised in that:In step (5), after transplanting, The plant of seedling is away from being 35~50cm for 25~40cm, line-spacing in the plantation field.
10. seed selection and the cultural method of the fleece-flower root according to claim 7, it is characterised in that:Also wrapped after step (5) Include:When the seedling grows to 25~50cm, it is climing to leave 1~2 strong Teng, erases remaining Teng is climing or cuts, so Afterwards after the furrow face in Teng climing covering full plantation, remove thin and delicate Teng is climing.
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