CN107484693A - A kind of discriminating of top shell sex and labeling method - Google Patents
A kind of discriminating of top shell sex and labeling method Download PDFInfo
- Publication number
- CN107484693A CN107484693A CN201710838268.6A CN201710838268A CN107484693A CN 107484693 A CN107484693 A CN 107484693A CN 201710838268 A CN201710838268 A CN 201710838268A CN 107484693 A CN107484693 A CN 107484693A
- Authority
- CN
- China
- Prior art keywords
- top shell
- shell
- land
- sex
- spiral case
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000237983 Trochidae Species 0.000 title claims abstract description 52
- 238000002372 labelling Methods 0.000 title claims abstract description 18
- 230000005856 abnormality Effects 0.000 claims abstract description 3
- 210000004907 gland Anatomy 0.000 claims description 20
- 239000013535 sea water Substances 0.000 claims description 15
- 239000011521 glass Substances 0.000 claims description 14
- 210000002149 gonad Anatomy 0.000 claims description 13
- 239000004568 cement Substances 0.000 claims description 11
- 239000000463 material Substances 0.000 claims description 11
- 239000003550 marker Substances 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 5
- 230000003187 abdominal effect Effects 0.000 claims description 5
- 239000003086 colorant Substances 0.000 claims description 2
- 238000007711 solidification Methods 0.000 claims description 2
- 230000008023 solidification Effects 0.000 claims description 2
- 230000004936 stimulating effect Effects 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 17
- 238000009395 breeding Methods 0.000 abstract description 9
- 230000001488 breeding effect Effects 0.000 abstract description 9
- 230000008569 process Effects 0.000 abstract description 7
- 230000000007 visual effect Effects 0.000 abstract description 2
- 230000001568 sexual effect Effects 0.000 description 15
- 239000003292 glue Substances 0.000 description 6
- 235000014653 Carica parviflora Nutrition 0.000 description 3
- 241000243321 Cnidaria Species 0.000 description 3
- 241000289291 Tectus pyramis Species 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/90—Sorting, grading, counting or marking live aquatic animals, e.g. sex determination
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Of Seaweed (AREA)
- Artificial Fish Reefs (AREA)
Abstract
The invention discloses a kind of discriminating of top shell sex and labeling method, it includes spiral shell and selects, sex abnormality, mark and temporarily supports four steps.The inventive method outfit is simple, operating process is easily grasped, differentiate and labeling process is apparent, visual, process will not cause substantial damage to breechblock, available for reasonable selection in artificial breeding is female, male spiral shell ratio, and then ensure nursery success rate, improve breeding efficiency.
Description
Technical field
The invention belongs to technical field of aquaculture, it is more particularly related to a kind of discriminating of top shell sex
And labeling method.
Background technology
Top shell is a kind of valuable tropical invertebrate, is distributed mainly on the whole Indian Ocean to Western Pacific
Tropical waters, including China's South China Sea.Due to its delicious flavour, nutritive value is high, is the important foodstuffs source of locals, together
Shi Zuowei outlets marine product, handicraft raw material, and the Important Economic source of locals.In recent years due to excessively being caught to top shell
Drag for, its natural survival volume is increasingly reduced.
Meanwhile China greatly develops and built Maritime Silk Road, safeguard the marine eco-environment with keep ocean
The ecological balance is the important problem that must be solved in evolution.Due to Environmental variations and the effect of human activity, China
The destructiveness of coral reef ecologic system increasingly sharpen；Important composition species of the top shell as coral reef ecologic system, protect
The stabilization of its biomass is held, is had great significance to protecting and repairing coral reef ecologic system.And before carrying out restoration of the ecosystem
It is sufficient seed supply to carry, so it is very necessary to carry out artificial breeding to top shell.
The current research to top shell both at home and abroad is very few, and only few research is related to top shell artificial breeding, at present state
It is inside and outside also without formed more perfect top shell artificial seedling rearing's method.Commonly required male during shellfish hatchery
Bred less, it is necessary to select a certain proportion of female, male individual；The sex of top shell belongs to gonochorism type, but from profile
Its sex can not be differentiated, so as to can not reasonably select female, male spiral shell to carry out nursery, easily cause nursery failure, breeding efficiency is low.
So primarily solve the problems, such as to be how that the sex of spiral shell is differentiated and marked during top shell artificial breeding, so as to
Reasonable employment is female, male spiral shell, improves the success rate of nursery.It can be seen that it is badly in need of a kind of easy, easy-operating sex mirror in seedling raising process
Other and labeling method.
The content of the invention
It is an object of the invention to：Overcome the problems such as being difficult to during existing top shell artificial breeding differentiate the sex of spiral shell,
Discriminating and the labeling method of a kind of easy, easy-operating top shell sex are provided.
In order to realize foregoing invention purpose, the invention provides a kind of discriminating of top shell sex and labeling method, and it is wrapped
Include following steps：
(1) spiral shell is selected：Select the complete top shell of spiral case；
(2) sex abnormality：Top shell is placed according to the direction of land (spire most on one layer of gyration) upward, touch
Land, the gonad in land is shunk to spiral case base portion, land is then cut down into (cutting angle is perpendicular to spiral shell axle), seen
To top gyration in cavity-like (it be in cavity-like to have not yet to see gyration if any individual, repeatable above procedure to visible)；From spiral case base
Portion stimulates the abdominal foot of top shell, gonad top is moved to spiral case top；When spiral case push pipe sees life in the gyration of cavity-like
When growing gland, have blackish green gonad for female top shell individual, have white gonad for male top shell individual；
(3) mark：By the individual and male top shell individual marker material of female top shell by its top in cavity-like
Gyration is filled and sealed；
(4) temporarily support：The top shell marked is placed in the container equipped with seawater according to the direction of land upward and temporarily supported,
Treat the marker material solidification.
Discriminating and labeling method as top shell sex of the present invention, in step (1), the complete top shell of spiral case is
Clean no-sundries and the phenomenon that suture is high-visible, each gyration is built again without obvious damaged and land without breakage in spiral case.
Discriminating and labeling method as top shell sex of the present invention, it is first right before marker material in step (3)
The individual and male top shell individual of female top shell is handled, including：The land cut place of spiral case is dried, stimulation push pipe is in
The gonad of cavity-like gyration, it is set to spiral case base portion to be contracted to top invisible.
Discriminating and labeling method as top shell sex of the present invention, in step (3), the marker material is different colours
Glass cement.
Discriminating and labeling method as top shell sex of the present invention, in step (4), the seawater is through sand filter and dark place
The natural sea-water of reason, depth are the 1/3 of top shell height.
Relative to prior art, the inventive method outfit is simple, operating process is easily grasped, and differentiates and labeling process table
See, be visual, process will not cause substantial damage to breechblock, available for reasonable selection in artificial breeding is female, male spiral shell ratio, enter
And ensure nursery success rate, improve breeding efficiency.
Embodiment
In order that the purpose of the present invention, technical scheme and advantageous effects become apparent from, with reference to embodiments, to this
Invention is further elaborated.It should be appreciated that the embodiment described in this specification is just for the sake of this hair of explanation
Bright, being not intended to limit the present invention, the parameter of embodiment, ratio etc. can suit measures to local conditions to make a choice and have no substance to result
Influence.
Embodiment 1
(1) using the top shell (Tectus pyramis) in the collection of Shenzhen Nan'ao marine site, after clearing up spiral case, spiral shell is selected
100 conducts of spiral shell of the clean no-sundries of shell and suture is high-visible, gyration is built again without obvious damaged, land without breakage phenomenon
Test material (spiral shell is high and spiral shell is respectively 60.43 ± 4.65mm, 79.02 ± 7.26g again).
(2) spiral shell selected is placed upwards by land, slowly touches land 5~6 times from upper with hammer, make the reproduction in land
Gland shrinks to base portion.Tight breechblock spire is then held, spiral shell axle is parallel with desktop, and breechblock is adjacent to desktop, and shell top is stretched out into table
Face is hanging.With hand held power saw perpendicular to spiral shell axle, shell top is cut down, it is seen that top gyration is in cavity-like；Some individuals are not yet
It is in cavity-like to see gyration, then repeats above procedure to visible.Its abdominal foot immediately is stimulated from base portion with hand, its gonad top
It can move up, until top is in the visible sexual gland of cavity-like gyration.The spiral shell finally can be identified according to the color of sexual gland
Sex, have blackish green sexual gland for female individuals, have white sexual gland for male.
(3) after identifying sex, the seawater around cut place is dried with dry towel, it is in cavity-like to stimulate top with finger
The sexual gland of gyration, it is set to be contracted to again to base portion invisible.It is in immediately cavity-like spiral shell by female land end with clear glass glue
Layer is filled and sealed, as mark；Male land end is filled and sealed in cavity-like gyration with white glass glue, as mark.
(4) spiral shell marked is placed on upwards by land and be equipped with the container of the natural sea-water through husky filter, dark treatment temporarily
Support.Sea water advanced about 1/3 spiral shell is high, to ensure to be in drying regime, glass cement after 10~12 hours for the glass cement marked
It is fully cured.
(5) spiral shell marked is put into cement pit and cultivated, bottom of pond portion, which is cultivated, benthic diatom, micro- inflation in pond.One
The spiral shell of each group is normally ingested in week, no death condition, illustrates that above-mentioned discriminating and marking operation will not cause death to breechblock, method
Safe and feasible.
Embodiment 2
(1) using the top shell (Tectus pyramis) in the collection of Shenzhen Nan'ao marine site, after clearing up spiral case, spiral shell is selected
100 conducts of spiral shell of the clean no-sundries of shell and suture is high-visible, gyration is built again without obvious damaged, land without breakage phenomenon
Test material (spiral shell is high and spiral shell is respectively 53.1178 ± 3.402667mm, 56.5138 ± 7.885025g again).
(2) spiral shell selected is placed upwards by land, slowly touches land 5~6 times from upper with hammer, make the reproduction in land
Gland shrinks to base portion.Tight breechblock spire is then held, spiral shell axle is parallel with desktop, and breechblock is adjacent to desktop, and shell top is stretched out into table
Face is hanging.With hand held power saw perpendicular to spiral shell axle, shell top is cut down, it is seen that top gyration is in cavity-like；Some individuals are not yet
It is in cavity-like to see gyration, then repeats above procedure to visible.Its abdominal foot immediately is stimulated from base portion with hand, its gonad top
It can move up, until top is in the visible sexual gland of cavity-like gyration.The spiral shell finally can be identified according to the color of sexual gland
Sex, have blackish green sexual gland for female individuals, have white sexual gland for male.
(3) after identifying sex, the seawater around cut place is dried with dry towel, it is in cavity-like to stimulate top with finger
The sexual gland of gyration, it is set to be contracted to again to base portion invisible.It is in immediately cavity-like spiral shell by female land end with clear glass glue
Layer is filled and sealed, as mark；Male land end is filled and sealed in cavity-like gyration with white glass glue, as mark.
(4) spiral shell marked is placed on upwards by land and be equipped with the container of the natural sea-water through husky filter, dark treatment temporarily
Support.Sea water advanced about 1/3 spiral shell is high, to ensure to be in drying regime, glass cement after 10~12 hours for the glass cement marked
It is fully cured.
(5) spiral shell marked is put into cement pit and cultivated, bottom of pond portion, which is cultivated, benthic diatom, micro- inflation in pond.One
The spiral shell of each group is normally ingested in week, no death condition, illustrates that above-mentioned discriminating and marking operation will not cause death to breechblock, method
Safe and feasible.
Embodiment 3
(1) using the top shell (Tectus pyramis) in the collection of Shenzhen Nan'ao marine site, after clearing up spiral case, spiral shell is selected
100 conducts of spiral shell of the clean no-sundries of shell and suture is high-visible, gyration is built again without obvious damaged, land without breakage phenomenon
Test material (spiral shell is high and spiral shell is respectively 43.47867 ± 3.216031mm, 32.31667 ± 7.503185g again).
(2) spiral shell selected is placed upwards by land, slowly touches land 5~6 times from upper with hammer, make the reproduction in land
Gland shrinks to base portion.Tight breechblock spire is then held, spiral shell axle is parallel with desktop, and breechblock is adjacent to desktop, and shell top is stretched out into table
Face is hanging.With hand held power saw perpendicular to spiral shell axle, shell top is cut down, it is seen that top gyration is in cavity-like；Some individuals are not yet
It is in cavity-like to see gyration, then repeats above procedure to visible.Its abdominal foot immediately is stimulated from base portion with hand, its gonad top
It can move up, until top is in the visible sexual gland of cavity-like gyration.The spiral shell finally can be identified according to the color of sexual gland
Sex, have blackish green sexual gland for female individuals, have white sexual gland for male.
(3) after identifying sex, the seawater around cut place is dried with dry towel, it is in cavity-like to stimulate top with finger
The sexual gland of gyration, it is set to be contracted to again to base portion invisible.It is in immediately cavity-like spiral shell by female land end with clear glass glue
Layer is filled and sealed, as mark；Male land end is filled and sealed in cavity-like gyration with white glass glue, as mark.
(4) spiral shell marked is placed on upwards by land and be equipped with the container of the natural sea-water through husky filter, dark treatment temporarily
Support.Sea water advanced about 1/3 spiral shell is high, to ensure to be in drying regime, glass cement after 10~12 hours for the glass cement marked
It is fully cured.
(5) spiral shell marked is put into cement pit and cultivated, bottom of pond portion, which is cultivated, benthic diatom, micro- inflation in pond.One
The spiral shell of each group is normally ingested in week, no death condition, illustrates that above-mentioned discriminating and marking operation will not cause death to breechblock, method
Safe and feasible.
The announcement and teaching of book according to the above description, those skilled in the art in the invention can also be to above-mentioned embodiment party
Formula carries out appropriate change and modification.Therefore, the invention is not limited in embodiment disclosed and described above, to this
Some modifications and changes of invention should also be as falling into the scope of the claims of the present invention.In addition, although this specification
In used some specific terms, but these terms are merely for convenience of description, do not form any restrictions to the present invention.
Claims (5)
1. discriminating and the labeling method of a kind of top shell sex, it is characterised in that comprise the following steps：
(1) spiral shell is selected：Select the complete top shell of spiral case；
(2) sex abnormality：Top shell is placed according to land direction upward, touches land, makes the gonad in land to spiral case
Base portion shrinks, and then cuts down land, it is in cavity-like to see top gyration；The abdominal foot of top shell is stimulated from spiral case base portion,
Gonad top is set to be moved to spiral case top；When spiral case push pipe sees gonad in the gyration of cavity-like, there is blackish green life
Grow gland for female top shell individual, have white gonad for male top shell individual；
(3) mark：Gyration by the individual and male top shell individual marker material of female top shell by its top in cavity-like
Fill and seal；
(4) temporarily support：The top shell marked is placed in the container equipped with seawater according to the direction of land upward and temporarily supported, treats institute
State marker material solidification.
2. discriminating and the labeling method of top shell sex according to claim 1, it is characterised in that described in step (1)
The complete top shell of spiral case be in spiral case clean no-sundries and suture is high-visible, each gyration without obvious damaged and land without
The phenomenon that breakage is built again.
3. discriminating and the labeling method of top shell sex according to claim 1, it is characterised in that in step (3), use
Before marker material, first individual to female top shell and male top shell individual is handled, including：The land of spiral case is cut
Place is dried, and the gonad for stimulating push pipe to be in cavity-like gyration, it to spiral case base portion is contracted to top invisible.
4. discriminating and the labeling method of top shell sex according to claim 1, it is characterised in that described in step (3)
Marker material is the glass cement of different colours.
5. discriminating and the labeling method of top shell sex according to claim 1, it is characterised in that described in step (4)
Seawater is the natural sea-water through husky filter and dark treatment, and depth is the 1/3 of top shell height.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710838268.6A CN107484693B (en) | 2017-09-18 | 2017-09-18 | A kind of identification and labeling method of top shell gender |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710838268.6A CN107484693B (en) | 2017-09-18 | 2017-09-18 | A kind of identification and labeling method of top shell gender |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107484693A true CN107484693A (en) | 2017-12-19 |
| CN107484693B CN107484693B (en) | 2019-06-11 |
Family
ID=60651802
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710838268.6A Active CN107484693B (en) | 2017-09-18 | 2017-09-18 | A kind of identification and labeling method of top shell gender |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107484693B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110074025A (en) * | 2019-05-05 | 2019-08-02 | 广西师范大学 | It is a kind of quickly to identify China and USA method for distinguishing |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003015508A1 (en) * | 2001-08-14 | 2003-02-27 | Mollusc Pty Ltd | A method of tagging a shellfish and a detectable shellfish produced by such a method |
| CN105454104A (en) * | 2015-09-25 | 2016-04-06 | 福建省水产研究所 | Babylonia spiral shell metal marking method |
| CN106359233A (en) * | 2016-11-11 | 2017-02-01 | 天津农学院 | Preparation method of traceable label carrying fresh and alive shellfish product information |
| CN106614216A (en) * | 2016-11-30 | 2017-05-10 | 中国水产科学研究院南海水产研究所 | Method for marking marine shellfishes by means of waterproof labels |
-
2017
- 2017-09-18 CN CN201710838268.6A patent/CN107484693B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003015508A1 (en) * | 2001-08-14 | 2003-02-27 | Mollusc Pty Ltd | A method of tagging a shellfish and a detectable shellfish produced by such a method |
| CN105454104A (en) * | 2015-09-25 | 2016-04-06 | 福建省水产研究所 | Babylonia spiral shell metal marking method |
| CN106359233A (en) * | 2016-11-11 | 2017-02-01 | 天津农学院 | Preparation method of traceable label carrying fresh and alive shellfish product information |
| CN106614216A (en) * | 2016-11-30 | 2017-05-10 | 中国水产科学研究院南海水产研究所 | Method for marking marine shellfishes by means of waterproof labels |
Non-Patent Citations (1)
| Title |
|---|
| 史兼华: "《海洋珍珠贝类遗传标记研究进展》", 《海洋通报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110074025A (en) * | 2019-05-05 | 2019-08-02 | 广西师范大学 | It is a kind of quickly to identify China and USA method for distinguishing |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107484693B (en) | 2019-06-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103875575B (en) | Breeding method for South American white shrimps in freshwater lake | |
| CN107897068B (en) | Method for improving proliferation and releasing survival rate of artificially bred tridacna juvenile mollusks | |
| CN103283641B (en) | A kind of hybridized prussian carp is at coastal saline-alkali water pond high-yield cultivation method | |
| CN101341851A (en) | Artificial algae field and construction method | |
| CN105557575A (en) | Breeding method for high-quality crab | |
| CN104686427A (en) | Intensive ecological culture method for trachidermus fasciatus | |
| CN105684878A (en) | Artificial ecological culturing method for caulerpa lentillifera | |
| Hwang et al. | Effects of stocking density on the cage culture of Korean rockfish (Sebastes schlegeli) | |
| CN104255613A (en) | Method of freshwater aquaculture of Litopenaeus vannamei in net cage | |
| CN104969889B (en) | A kind of method for pearl culture | |
| CN107484693A (en) | A kind of discriminating of top shell sex and labeling method | |
| CN206402949U (en) | A kind of food platform with aerating device | |
| CN102715104B (en) | Method for promoting maturation of pampus argenteus gonads | |
| CN104813968A (en) | Cultchless connection aquaculture method for improving economic benefit of Hong Kong oysters | |
| CN108271719A (en) | A kind of cultural method of haliotis discus hannai Ino | |
| CN103875556A (en) | Method for breeding abalone | |
| CN103262816A (en) | Equipment for cultivating Charybdis feriatus female parents and application method of equipment | |
| CN106106263A (en) | The mixed cultivation method of high yield Lobster | |
| CN106069935A (en) | The cultural method of Concha Ostreae | |
| CN101473803A (en) | All-weather induced rapid ovulation method of Chlamys nobilis | |
| CN111820161A (en) | Breeding device and method for polyculture of marsupenaeus japonicus and sea grapes | |
| CN104221839B (en) | The removing method of " monkey hair " in asparagus cultivation | |
| CN108064778A (en) | A kind of cultural method for improving mud crab survival rate | |
| CN105638538B (en) | A kind of lefteye flounder ecology mark method for being used to release statistics | |
| CN104206338A (en) | Artificial culture method for bastard halibuts |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |