CN107484693B - A kind of identification and labeling method of top shell gender - Google Patents
A kind of identification and labeling method of top shell gender Download PDFInfo
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- CN107484693B CN107484693B CN201710838268.6A CN201710838268A CN107484693B CN 107484693 B CN107484693 B CN 107484693B CN 201710838268 A CN201710838268 A CN 201710838268A CN 107484693 B CN107484693 B CN 107484693B
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- top shell
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- shell
- spiral case
- gyration
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/90—Sorting, grading, counting or marking live aquatic animals, e.g. sex determination
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- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Artificial Fish Reefs (AREA)
- Cultivation Of Seaweed (AREA)
Abstract
The invention discloses the identifications and labeling method of a kind of top shell gender comprising spiral shell selects, sex abnormality, label and temporarily supports four steps.The method of the present invention outfit is simple, operating process is easily grasped, identify and labeling process is apparent, visual, process will not cause substantive damage to breechblock, can be used in artificial breeding reasonably selecting female, male spiral shell ratio, and then guarantee nursery success rate, improve breeding efficiency.
Description
Technical field
The invention belongs to technical field of aquaculture, it is more particularly related to a kind of identification of top shell gender
And labeling method.
Background technique
Top shell is a kind of valuable tropical invertebrate, is distributed mainly on the entire Indian Ocean to Western Pacific
Tropical waters, including China's South China Sea.Due to its delicious flavour, it is the important foodstuffs source of locals, together that nutritive value is high
Shi Zuowei exports marine product, craftwork raw material and the Important Economic source of locals.In recent years due to excessively being caught to top shell
Fishing, natural survival volume are increasingly reduced.
Meanwhile China is greatly developing and is building Maritime Silk Road, safeguard the marine eco-environment with keep ocean
The ecological balance is the important problem that must be solved in development process.Due to Environmental variations and the effect of human activity, China
The extent of the destruction of coral reef ecologic system increasingly sharpen；Important composition species of the top shell as coral reef ecologic system are protected
The stabilization for holding its biomass has great significance to protecting and repairing coral reef ecologic system.And before carrying out restoration of the ecosystem
Mentioning is sufficient seed supply, so it is very necessary to carry out artificial breeding to top shell.
Very few to the research of top shell both at home and abroad at present, only few research is related to top shell artificial breeding, at present state
It is inside and outside to form more perfect top shell artificial seedling rearing's method not yet.Commonly required male during shellfish hatchery
It is few, it needs to select a certain proportion of female, male individual and is bred；The gender of top shell belongs to gonochorism type, but from shape
Its gender cannot be identified, so that female, male spiral shell cannot reasonably be selected to carry out nursery, be easy to cause nursery to fail, breeding efficiency is low.
So primarily solve the problems, such as to be how that the gender of spiral shell is identified and marked during top shell artificial breeding, so as to
Reasonable employment is female, male spiral shell, improves the success rate of nursery.As it can be seen that being badly in need of a kind of easy, easy-operating gender mirror in seedling raising process
Other and labeling method.
Summary of the invention
It is an object of the invention to: overcome existing top shell artificial breeding to be difficult to the problems such as identifying the gender of spiral shell in the process,
The identification and labeling method of a kind of easy, easy-operating top shell gender are provided.
In order to achieve the above-mentioned object of the invention, the present invention provides the identification and labeling method of a kind of top shell gender, packets
Include following steps:
(1) spiral shell is selected: selecting the complete top shell of spiral case；
(2) sex abnormality: top shell is placed according to the direction of land (spire most on one layer of gyration) upward, tap
Land shrinks the gonad in land to spiral case base portion, and land is then cut down (cutting angle is perpendicular to spiral shell axis), is seen
To top gyration in cavity-like (having not yet to see gyration in cavity-like if any individual, repeat above procedure to visible)；From spiral case base
Portion stimulates the abdominal foot of top shell, keeps gonad top mobile to spiral case top；When spiral case push pipe sees life in the gyration of cavity-like
When growing gland, having blackish green gonad is female top shell individual, and having white gonad is male top shell individual；
(3) it marks: by female top shell individual and male top shell individual marker material by its top in cavity-like
Gyration is filled and is sealed；
(4) it temporarily supports: the top shell marked being placed in the container equipped with seawater according to the direction of land upward and is temporarily supported,
Solidify to the marker material.
As the identification and labeling method of top shell gender of the present invention, in step (1), the complete top shell of spiral case is
Clean no-sundries and the phenomenon that suture is high-visible, each gyration is built without obvious damaged and land without breakage again in spiral case.
It is first right before marker material in step (3) as the identification and labeling method of top shell gender of the present invention
Female top shell individual and male top shell individual are handled, comprising: are dried the land cut place of spiral case, stimulation push pipe is in
The gonad of cavity-like gyration makes it to spiral case base portion be contracted to top invisible.
As the identification and labeling method of top shell gender of the present invention, in step (3), the marker material is different colours
Glass cement.
As the identification and labeling method of top shell gender of the present invention, in step (4), the seawater is through sand filter and dark place
The natural sea-water of reason, depth are the 1/3 of top shell height.
Compared with the existing technology, the method for the present invention outfit is simple, operating process is easily grasped, and identifies and labeling process table
It sees, is visual, process will not cause substantive damage to breechblock, it can be used in artificial breeding reasonably selecting female, male spiral shell ratio, into
And guarantee nursery success rate, improve breeding efficiency.
Specific embodiment
In order to be more clear the purpose of the present invention, technical solution and advantageous effects, with reference to embodiments, to this
Invention is further elaborated.It should be understood that embodiment described in this specification is just for the sake of this hair of explanation
It is bright, be not intended to limit the present invention, parameter, ratio of embodiment etc. can adaptation to local conditions make a choice and substance had no to result
It influences.
Embodiment 1
(1) spiral shell is selected after clearing up spiral case using the top shell (Tectus pyramis) acquired in Shenzhen, sea area, Nan'ao
The clean no-sundries of shell and 100 conducts of spiral shell that suture is high-visible, gyration is without obvious damaged, land without damaged the phenomenon that building again
Test material (spiral shell height and spiral shell are respectively 60.43 ± 4.65mm, 79.02 ± 7.26g again).
(2) spiral shell selected is placed upwards by land, with hammer slowly from upper tap land 5~6 times, makes the reproduction in land
Gland is shunk to base portion.Tight breechblock spire is then held, spiral shell axis is parallel with desktop, and breechblock is adjacent to desktop, and table is stretched out on shell top
Face is hanging.With hand held power saw perpendicular to spiral shell axis, shell top is cut down, it is seen that top gyration is in cavity-like；Some individuals are not yet
Gyration is seen in cavity-like, then repeats above procedure to visible.Immediately its abdominal foot, gonad top are stimulated from base portion with hand
It can move up, until top is in the visible sexual gland of cavity-like gyration.The spiral shell finally can be identified according to the color of sexual gland
Gender, having blackish green sexual gland is female individuals, and having white sexual gland is male.
(3) after identifying gender, the seawater around cut place is dried with dry towel, is in cavity-like with finger stimulation top
The sexual gland of gyration is contracted to it to base portion again invisible.It is in immediately cavity-like spiral shell by female land end with transparent glass glue
Layer is filled and is sealed, as label；Male land end is filled and sealed in cavity-like gyration with white glass glue, as label.
(4) spiral shell marked is placed on upwards by land in the container equipped with the natural sea-water through husky filter, dark treatment temporarily
It supports.Sea water advanced about 1/3 spiral shell is high, to guarantee that the glass cement for label is in drying regime, glass cement after 10~12 hours
It is fully cured.
(5) spiral shell marked is put into a cement pool cultivation, bottom of pond portion culture has benthic diatom, micro- inflation in pond.One
The spiral shell of each group is normally ingested in week, no death condition, illustrates that above-mentioned identification and marking operation will not cause death to breechblock, method
Safe and feasible.
Embodiment 2
(1) spiral shell is selected after clearing up spiral case using the top shell (Tectus pyramis) acquired in Shenzhen, sea area, Nan'ao
The clean no-sundries of shell and 100 conducts of spiral shell that suture is high-visible, gyration is without obvious damaged, land without damaged the phenomenon that building again
Test material (spiral shell height and spiral shell are respectively 53.1178 ± 3.402667mm, 56.5138 ± 7.885025g again).
(2) spiral shell selected is placed upwards by land, with hammer slowly from upper tap land 5~6 times, makes the reproduction in land
Gland is shunk to base portion.Tight breechblock spire is then held, spiral shell axis is parallel with desktop, and breechblock is adjacent to desktop, and table is stretched out on shell top
Face is hanging.With hand held power saw perpendicular to spiral shell axis, shell top is cut down, it is seen that top gyration is in cavity-like；Some individuals are not yet
Gyration is seen in cavity-like, then repeats above procedure to visible.Immediately its abdominal foot, gonad top are stimulated from base portion with hand
It can move up, until top is in the visible sexual gland of cavity-like gyration.The spiral shell finally can be identified according to the color of sexual gland
Gender, having blackish green sexual gland is female individuals, and having white sexual gland is male.
(3) after identifying gender, the seawater around cut place is dried with dry towel, is in cavity-like with finger stimulation top
The sexual gland of gyration is contracted to it to base portion again invisible.It is in immediately cavity-like spiral shell by female land end with transparent glass glue
Layer is filled and is sealed, as label；Male land end is filled and sealed in cavity-like gyration with white glass glue, as label.
(4) spiral shell marked is placed on upwards by land in the container equipped with the natural sea-water through husky filter, dark treatment temporarily
It supports.Sea water advanced about 1/3 spiral shell is high, to guarantee that the glass cement for label is in drying regime, glass cement after 10~12 hours
It is fully cured.
(5) spiral shell marked is put into a cement pool cultivation, bottom of pond portion culture has benthic diatom, micro- inflation in pond.One
The spiral shell of each group is normally ingested in week, no death condition, illustrates that above-mentioned identification and marking operation will not cause death to breechblock, method
Safe and feasible.
Embodiment 3
(1) spiral shell is selected after clearing up spiral case using the top shell (Tectus pyramis) acquired in Shenzhen, sea area, Nan'ao
The clean no-sundries of shell and 100 conducts of spiral shell that suture is high-visible, gyration is without obvious damaged, land without damaged the phenomenon that building again
Test material (spiral shell height and spiral shell are respectively 43.47867 ± 3.216031mm, 32.31667 ± 7.503185g again).
(2) spiral shell selected is placed upwards by land, with hammer slowly from upper tap land 5~6 times, makes the reproduction in land
Gland is shunk to base portion.Tight breechblock spire is then held, spiral shell axis is parallel with desktop, and breechblock is adjacent to desktop, and table is stretched out on shell top
Face is hanging.With hand held power saw perpendicular to spiral shell axis, shell top is cut down, it is seen that top gyration is in cavity-like；Some individuals are not yet
Gyration is seen in cavity-like, then repeats above procedure to visible.Immediately its abdominal foot, gonad top are stimulated from base portion with hand
It can move up, until top is in the visible sexual gland of cavity-like gyration.The spiral shell finally can be identified according to the color of sexual gland
Gender, having blackish green sexual gland is female individuals, and having white sexual gland is male.
(3) after identifying gender, the seawater around cut place is dried with dry towel, is in cavity-like with finger stimulation top
The sexual gland of gyration is contracted to it to base portion again invisible.It is in immediately cavity-like spiral shell by female land end with transparent glass glue
Layer is filled and is sealed, as label；Male land end is filled and sealed in cavity-like gyration with white glass glue, as label.
(4) spiral shell marked is placed on upwards by land in the container equipped with the natural sea-water through husky filter, dark treatment temporarily
It supports.Sea water advanced about 1/3 spiral shell is high, to guarantee that the glass cement for label is in drying regime, glass cement after 10~12 hours
It is fully cured.
(5) spiral shell marked is put into a cement pool cultivation, bottom of pond portion culture has benthic diatom, micro- inflation in pond.One
The spiral shell of each group is normally ingested in week, no death condition, illustrates that above-mentioned identification and marking operation will not cause death to breechblock, method
Safe and feasible.
According to the disclosure and teachings of the above specification, those skilled in the art in the invention can also be to above-mentioned embodiment party
Formula carries out change and modification appropriate.Therefore, the invention is not limited to the specific embodiments disclosed and described above, to this
Some modifications and changes of invention should also be as falling into the scope of the claims of the present invention.In addition, although this specification
In use some specific terms, these terms are merely for convenience of description, does not limit the present invention in any way.
Claims (5)
1. a kind of identification and labeling method of top shell gender, which comprises the steps of:
(1) spiral shell is selected: selecting the complete top shell of spiral case；
(2) sex abnormality: top shell is placed according to the direction of land upward, land is tapped, makes the gonad in land to spiral case
Base portion is shunk, and then cuts down land, sees top gyration in cavity-like；The abdominal foot of top shell is stimulated from spiral case base portion,
Keep gonad top mobile to spiral case top；When spiral case push pipe sees gonad in the gyration of cavity-like, there is blackish green life
Growing gland is female top shell individual, and having white gonad is male top shell individual；
(3) it marks: the gyration by female top shell individual and male top shell individual marker material by its top in cavity-like
It fills and seals；
(4) it temporarily supports: the top shell marked being placed in the container equipped with seawater according to the direction of land upward and is temporarily supported, to institute
State marker material solidification.
2. the identification and labeling method of top shell gender according to claim 1, which is characterized in that described in step (1)
The complete top shell of spiral case be in spiral case clean no-sundries and suture is high-visible, each gyration without obvious damaged and land without
The phenomenon that breakage is built again.
3. the identification and labeling method of top shell gender according to claim 1, which is characterized in that in step (3), use
Before marker material, first female top shell individual and male top shell individual are handled, comprising: cut the land of spiral case
Place is dried, and stimulation push pipe is in the gonad of cavity-like gyration, so that it to spiral case base portion is contracted to top invisible.
4. the identification and labeling method of top shell gender according to claim 1, which is characterized in that described in step (3)
Marker material is the glass cement of different colours.
5. the identification and labeling method of top shell gender according to claim 1, which is characterized in that described in step (4)
Seawater is the natural sea-water through husky filter and dark treatment, and depth is the 1/3 of top shell height.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710838268.6A CN107484693B (en) | 2017-09-18 | 2017-09-18 | A kind of identification and labeling method of top shell gender |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710838268.6A CN107484693B (en) | 2017-09-18 | 2017-09-18 | A kind of identification and labeling method of top shell gender |
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| CN107484693A CN107484693A (en) | 2017-12-19 |
| CN107484693B true CN107484693B (en) | 2019-06-11 |
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| CN110074025B (en) * | 2019-05-05 | 2020-05-12 | 广西师范大学 | Method for rapidly identifying sex of Chinese river snails |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003015508A1 (en) * | 2001-08-14 | 2003-02-27 | Mollusc Pty Ltd | A method of tagging a shellfish and a detectable shellfish produced by such a method |
| CN105454104A (en) * | 2015-09-25 | 2016-04-06 | 福建省水产研究所 | Babylonia spiral shell metal marking method |
| CN106359233A (en) * | 2016-11-11 | 2017-02-01 | 天津农学院 | Preparation method of traceable label carrying fresh and alive shellfish product information |
| CN106614216A (en) * | 2016-11-30 | 2017-05-10 | 中国水产科学研究院南海水产研究所 | Method for marking marine shellfishes by means of waterproof labels |
-
2017
- 2017-09-18 CN CN201710838268.6A patent/CN107484693B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003015508A1 (en) * | 2001-08-14 | 2003-02-27 | Mollusc Pty Ltd | A method of tagging a shellfish and a detectable shellfish produced by such a method |
| CN105454104A (en) * | 2015-09-25 | 2016-04-06 | 福建省水产研究所 | Babylonia spiral shell metal marking method |
| CN106359233A (en) * | 2016-11-11 | 2017-02-01 | 天津农学院 | Preparation method of traceable label carrying fresh and alive shellfish product information |
| CN106614216A (en) * | 2016-11-30 | 2017-05-10 | 中国水产科学研究院南海水产研究所 | Method for marking marine shellfishes by means of waterproof labels |
Non-Patent Citations (1)
| Title |
|---|
| 《海洋珍珠贝类遗传标记研究进展》;史兼华;《海洋通报》;20060630;80-86 * |
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