CN102669021B - Method for temporary culture, egg laying and egg protecting of octopus variabilis sasaki and device for method - Google Patents
Method for temporary culture, egg laying and egg protecting of octopus variabilis sasaki and device for method Download PDFInfo
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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Abstract
本发明公开了一种长蛸暂养、产卵和护卵的方法及所用装置,所述的方法包括有1)亲体的选择、2)亲体暂养促熟和3)亲体护卵步骤;且采用特殊设计的蛸巢。本发明的方法操作简单,所用装置陈本低、利用率高,能有效提高长蛸亲体的成活率、产卵量及受精卵的孵化率和幼苗的健康程度。并且本发明的蛸巢模拟长蛸自然巢穴结构,更适合亲蛸的隐蔽、拒敌及产卵、护卵等行为,而且蛸巢制作简单,使用方便,利用率高,是一种投资少、效果好的长蛸蛸巢。
The invention discloses a method for temporarily raising, laying eggs and protecting eggs of long octopus and the device used. The method includes 1) selection of parents, 2) temporary raising of parents to promote maturation and 3) steps of protecting eggs; and A specially designed octopus nest is used. The method of the invention is simple to operate, and the device used has low cost and high utilization rate, and can effectively improve the survival rate of the long octopus parent body, the amount of eggs laid, the hatching rate of fertilized eggs and the health of the seedlings. And the octopus nest of the present invention simulates the natural nest structure of the long octopus, which is more suitable for the behaviors of concealing, resisting enemies, laying eggs, and protecting eggs for close octopuses. Moreover, the octopus nest is simple to make, easy to use, and has a high utilization rate. It is a low-investment, A long octopus nest with good effect.
Description
技术领域 technical field
本发明属于水产养殖技术领域,具体涉及一种长蛸暂养、产卵和护卵的方法及所用装置。 The invention belongs to the technical field of aquaculture, and in particular relates to a method for temporarily raising long octopuses, laying eggs and protecting eggs and a device used therefor. the
背景技术 Background technique
长蛸(Octopus minor)隶属头足纲、八腕目、蛸科、蛸属,俗称马蛸、大蛸或八爪鱼,广泛分布于我国的渤海、黄海、东海、南海以及日本海域。长蛸一年生,在北方其繁殖高峰期在4~7月份。长蛸味道鲜美,营养价值丰富,大量出口日本、韩国,是我国北方重要的创汇海珍品。 Octopus minor belongs to Cephalopoda, Octopus order, Octopus family, Octopus genus, commonly known as horse octopus, giant octopus or octopus, and is widely distributed in the Bohai Sea, Yellow Sea, East China Sea, South China Sea and Japan Sea in my country. The long octopus is an annual, and its breeding peak in the north is from April to July. The long octopus is delicious and has rich nutritional value. It is exported to Japan and South Korea in large quantities. It is an important foreign exchange-earning treasure in northern my country. the
长蛸在人工暂养和亲体培育期间,一般采用PVC管或瓦片作为蛸巢(钱耀森,2011)。PVC管两端开口,内径一致,常出现多只长蛸挤在同一巢穴的现象,造成长蛸互相争斗和残食,不利于暂养和促熟,更不利于护卵。瓦片与自然蛸巢差别较大,没有形成洞穴结构,长蛸产卵后卵群更易受外界干扰。依据对自然生态环境下长蛸繁殖习性及其巢穴的观察,设计了适合其栖息繁育的蛸巢,并以此建立了一套技术体系和方法,以满足人工育苗、增养殖及放流需要。 Long octopuses generally use PVC pipes or tiles as octopus nests during artificial temporary breeding and broodstock cultivation (Qian Yaosen, 2011). Both ends of the PVC pipe are open and have the same inner diameter. Many octopuses often squeeze into the same nest, causing the octopuses to fight each other and cannibalize each other, which is not conducive to temporary breeding and maturation, and is not conducive to egg protection. There is a big difference between the tile and the natural octopus nest, and no cave structure is formed. After the long octopus lays eggs, the egg group is more easily disturbed by the outside world. Based on the observation of the breeding habits and nests of long octopuses in the natural ecological environment, the octopus nests suitable for their habitat and breeding were designed, and a set of technical systems and methods were established to meet the needs of artificial seedlings, breeding and release. the
发明内容 Contents of the invention
本发明的目的是提供一种长蛸暂养、产卵和护卵的方法及所用装置,解决现有条件下长蛸亲体互相残食以及在护卵期间相互残食受精卵等问题,以弥补现有技术的不足。 The object of the present invention is to provide a kind of long octopus temporary support, method and device used for laying eggs and egg protection, solve the problems such as mutual cannibalism of long octopus parents and mutual cannibalism during egg protection under the existing conditions, to make up for Insufficiency of existing technology. the
本发明的方法,包括如下步骤: The method of the present invention comprises the steps of:
1)亲体的选择:在每年的4~5月份从自然海区采捕长蛸,挑选体表完整、胴体部饱满、体色健康的作为亲体; 1) Selection of broodstock: Harvest long octopuses from natural sea areas from April to May every year, and select those with complete body surface, plump carcass, and healthy body color as the broodstock;
2)亲体暂养促熟:将亲体在暂养池中进行暂养促熟,在暂养池中投放蛸巢,蛸巢的数量为亲体的2倍;暂养池初始水温14~16℃,盐度28~32‰;暂养池光照强度为不超过500Lx;每两天升温1℃,升温至23~26℃时恒定,每天投喂饵料,直至亲体产卵; 2) Temporary raising of the parent body to promote ripening: the parent body is temporarily raised in the temporary raising pool to promote ripening, and octopus nests are placed in the temporary raising pool. The number of octopus nests is twice that of the parent body; The salinity is 28-32‰; the light intensity of the holding pond is not more than 500Lx; the temperature is raised by 1°C every two days, and when the temperature rises to 23-26°C, it is constant, and the bait is fed every day until the brood spawns;
3)亲体护卵:亲体产卵后将装有亲体的蛸巢移入孵化池,用筛网封住蛸巢开口端,使亲蛸在蛸巢中护卵,防止亲蛸从蛸巢中逃逸;孵化池水温调节为24℃恒定,调节光照强度不超过300Lx,流水培养并投喂饵料。每天检查受精卵 情况,及时清除坏卵、死卵。 3) Egg protection by the parent body: after the parent body lays eggs, move the octopus nest with the parent body into the hatching pool, seal the opening end of the octopus nest with a screen, so that the parent octopus can protect the eggs in the octopus nest, and prevent the parent octopus from escaping from the octopus nest; The water temperature of the hatching tank is adjusted to be constant at 24°C, the light intensity is adjusted to not exceed 300Lx, and the water is cultivated and bait is fed. Check the condition of fertilized eggs every day, and remove bad eggs and dead eggs in time. the
对于饵料投喂,在亲体暂养促熟期间,饵料为沙蚕、招潮蟹、肉球近方蟹、菲律宾蛤仔、杂色蛤和四角蛤蜊以及冰鲜白虾,其中各种饵料的比例为白虾30%,蟹类30%,蛤类20%,沙蚕20%,待亲蛸即将产卵时,将饵料中蟹类比例减小至10%,蛤类和沙蚕的比例提高到30%。亲体护卵期间,继续投饵,饵料为去掉螯足的蟹类和去掉一半外壳的蛤类,补充亲体能量,提高亲体护卵质量。 For bait feeding, during the temporary maturation period of broodstock, the bait is lugworm, fiddler crab, meat ball near crab, Philippine clam, variegated clam and square clam, and chilled white shrimp, and the ratio of various baits is white Shrimp 30%, crab 30%, clam 20%, clam worm 20%, when the parent octopus is about to lay eggs, reduce the proportion of crab in the bait to 10%, and increase the ratio of clam and clam worm to 30%. During the brood protection period, continue to cast bait. The bait is crabs with chelated feet removed and clams with half of the shell removed to supplement the energy of the broods and improve the quality of the broods. the
本发明所用的蛸巢,包括透水筒和护卵筒两个部分,所述的透水筒为圆筒状,前端开口,后端连接在护卵筒上;且透水筒的筒体上开有小孔;所述的护卵筒的后端为封闭的圆锥状。 The octopus nest used in the present invention includes two parts: a water-permeable cylinder and an egg-protecting cylinder. The water-permeable cylinder is cylindrical, with an open front end and a rear end connected to the egg-protecting cylinder; hole; the rear end of the egg protector is a closed conical shape. the
上述的透水筒的前端装有一个用于增加重量的不锈钢环;小孔直径为0.8cm,小孔间距为1.5cm。 The front end of the above-mentioned permeable cylinder is equipped with a stainless steel ring for increasing the weight; the diameter of the small holes is 0.8cm, and the distance between small holes is 1.5cm. the
上述护卵筒的后端连接有一个陶器坠。 A pottery pendant is connected to the rear end of the above-mentioned egg protector. the
该蛸巢总长为50~70cm,直径10~15cm。 The octopus nest has a total length of 50-70 cm and a diameter of 10-15 cm. the
本发明具有如下的优点: The present invention has the following advantages:
1、模拟生态条件下长蛸巢穴结构,使用无菌材料制备蛸巢,具有结实、抗腐蚀、抗磨损、价格低廉等特点,便于大规模推广应用,而且使长蛸合理分布,确保产卵后的亲体彼此隔离,为产卵、护卵创造稳定环境,避免外界干扰和亲体彼此间残食和争斗,可以提高长蛸的产卵量和孵化率。 1. Simulate the structure of the long octopus nest under ecological conditions, and use sterile materials to prepare the octopus nest, which has the characteristics of firmness, corrosion resistance, wear resistance, and low price, which is convenient for large-scale promotion and application, and makes the long octopus reasonable distribution to ensure The broodstock are isolated from each other to create a stable environment for laying eggs and protecting eggs, avoiding external interference and cannibalism and fighting among the broodstock, which can increase the spawning rate and hatching rate of long octopuses. the
2、整个培育过程,饵料搭配合理。暂养促熟阶段,蟹等甲壳类在饵料中占较大比例,大大促进长蛸亲体的性腺发育和成熟度,提高产卵量和受精卵质量;护卵期间,亲蛸活力减弱,减少蟹类的比例,以及提供去掉螯足的蟹类,避免蟹类攻击亲蛸,防止蟹类破坏受精卵和亲体,同时投喂去掉一半外壳的蛤类,使护卵亲体更容易摄食,有效地补充能量,进行长时间护卵。这对于提高幼体孵化率和成活率起到关键作用。 2. During the whole breeding process, the bait mix is reasonable. In the stage of temporary breeding and maturation promotion, crustaceans such as crabs account for a large proportion of the bait, which greatly promotes the gonad development and maturity of the parent octopus, improves the amount of eggs laid and the quality of fertilized eggs; during the egg protection period, the activity of the parent octopus weakens, reducing the The proportion of species, as well as providing crabs with their chelater feet removed, avoiding crabs attacking broodstock and preventing crabs from destroying fertilized eggs and broodstock. Energy for long-term egg protection. This plays a key role in improving the hatchability and survival rate of larvae. the
3、此外,护卵期间,采取控温、控光、流水培养等技术手段,都是依据对长蛸生态环境详细调查和分析的基础上获得的数据,为亲蛸和受精卵建立了一套稳定适宜的生态条件。及时清除坏卵、死卵,也为受精卵的发育创造有利条件。整套培育体系,易于长蛸规模化育苗和示范推广。 3. In addition, during the egg protection period, technical means such as temperature control, light control, and running water culture were adopted, all based on the data obtained from the detailed investigation and analysis of the ecological environment of the long octopus, and a set of breeding methods was established for the parent octopus and fertilized eggs. Stable and suitable ecological conditions. Timely removal of bad eggs and dead eggs also creates favorable conditions for the development of fertilized eggs. The whole set of cultivation system is easy for large-scale octopus seedling cultivation and demonstration and promotion. the
本蛸巢模拟长蛸自然巢穴结构,更适合亲蛸的隐蔽、拒敌及产卵、护卵等 行为,而且蛸巢制作简单,使用方便,利用率高,是一种投资少、效果好的长蛸蛸巢。 This octopus nest simulates the natural nest structure of the long octopus, which is more suitable for the concealment, enemy resistance, egg laying, egg protection and other behaviors of the parent octopus. Moreover, the octopus nest is simple to make, easy to use, and has high utilization rate. It is a kind of low investment and good effect. Long octopus nest. the
附图说明 Description of drawings
图1:为本发明的蛸巢结构示意图; Fig. 1: is the schematic diagram of octopus nest structure of the present invention;
图2:为本发明蛸巢的分解图; Fig. 2: is the exploded view of octopus nest of the present invention;
其中:1、透水筒 2、护卵筒 3、小孔 4、不锈钢环 5、陶器坠。
Among them: 1. Water-
具体实施方式 Detailed ways
本发明长蛸亲体暂养、产卵和护卵的方法具体步骤如下: The specific steps of the method for the long octopus parent body of the present invention to temporarily raise, lay eggs and protect eggs are as follows:
1)亲体的选择:挑选胴体体表完整、胴体饱满、体色健康的长蛸作为亲体;同时制备蛸巢:蛸巢为无菌塑料制成,由两段构成,前段呈圆筒状结构的透水筒1,后段呈圆锥状结构的护卵筒2,总长50~70cm,直径为10~15cm。透水筒1前端开口,管壁上镶嵌一枚不锈钢环4;护卵筒2的后段逐渐变窄,末端封闭,末端捆绑陶器坠5,陶瓷坠5重100~200g。透水筒1中空的筒状结构开口端的前三分之二处的管壁具有小孔3,小孔3直径为0.8cm,小孔3间距为1.5cm
1) Selection of parent body: select long octopus with complete carcass surface, plump carcass, and healthy body color as parent; prepare octopus nest at the same time: octopus nest is made of aseptic plastic, consisting of two sections, the front section is cylindrical. Permeable tube 1, rear section is the
2)亲体暂养促熟:将亲体在暂养池中进行暂养促熟,水温为14~16℃,盐度为28~32‰的暂养池,向暂养池投放本专利设计的蛸巢,数量为亲体的2倍,放入长蛸亲体,暂养池每两天升温1℃,升温至23~26℃后保持恒温,暂养车间用黑布遮光,调节光照强度不超过500Lx,暂养期间每天17:00投喂沙蚕、招潮蟹、肉球近方蟹、菲律宾蛤仔、杂色蛤、四角蛤蜊、冰鲜白虾等饵料,投喂量为长蛸亲体体重的3%~10%,长蛸最喜欢吃虾蟹类,各种饵料的比例为白虾30%,蟹类30%,蛤类20%,沙蚕20%。第二天上午清理残饵,暂养期间,每2天换水1/2。长蛸亲体即将产卵时,活力减弱,此时应将饵料中蟹比例减少至10%,防止蟹类攻击亲体,将沙蚕和蛤类的比例提高至30%。通过饵料调整,可以保证亲体在暂养促熟期间既能有充足的活饵,又不会在进食时消耗额外的能量,促使亲体的性腺发育更好,提高产卵量。 2) Temporary raising of the parent body to promote ripening: Put the parent body in the temporary raising pond for temporary raising to promote ripening. The water temperature is 14-16°C and the salinity is 28-32‰, and the octopus designed by this patent is put into the temporary raising pond The number of nests is twice that of the broodstock. Put the octopus broodstock in. The temperature of the holding pool is raised by 1°C every two days, and then kept at a constant temperature after the temperature rises to 23-26°C. The holding workshop is shaded with black cloth, and the light intensity is adjusted to no more than 500Lx. During the temporary breeding period, feed clam worms, fiddler crabs, meat ball close crabs, Philippine clams, variegated clams, four-cornered clams, and chilled white shrimps every day at 17:00. 10%, octopus likes to eat shrimp and crabs most, and the proportion of various baits is white shrimp 30%, crab 30%, clams 20%, and clam worm 20%. Clean up the residual bait the next morning, and change 1/2 of the water every 2 days during the temporary breeding period. When the octopus parent is about to lay eggs, its vitality weakens. At this time, the proportion of crabs in the bait should be reduced to 10% to prevent crabs from attacking the parent, and the proportion of clams and clams should be increased to 30%. By adjusting the bait, it is possible to ensure that the broodstock can have sufficient live bait during the temporary maturation promotion period, and will not consume extra energy when eating, so as to promote the better development of the gonads of the broodstock and increase the amount of eggs laid. the
3)亲体护卵:当发现亲体产卵时,立即将蛸巢开口端用皮筋和筛网封住,并转移至孵化池,使亲蛸相互隔离,并防止其逃逸。护卵期间,亲蛸抵抗力减弱,应避免环境的频繁变化,将孵化池水温保持在24℃,并调节光照强度至300Lx以下,采用长流水进行培育,抑制细菌及其他敌害生物的滋生,并防止换水时 对亲蛸的刺激,提高护卵质量。护卵期间,亲蛸极少主动摄食。每隔2~3天投饵一次,维持亲体体能。投饵时将封住蛸巢的网取下来,将招潮蟹或肉球近方蟹剪断螯足,投入蛸巢中,或将菲律宾蛤仔、四角蛤蜊去掉一半外壳投入蛸巢中,每个蛸巢内投喂一个,再用网将蛸巢封好,放回孵化池,第二天清理残饵。在亲蛸护卵期间,每天检查受精卵情况,及时清除膨胀的坏卵、死卵。用此长蛸的护卵装置和方法,孵化率可达90%,高于一般方法的孵化率。 3) Egg protection by the parent body: When the parent body is found to be laying eggs, immediately seal the opening of the octopus nest with a rubber band and a screen, and transfer it to the hatching tank to isolate the parent octopus from each other and prevent them from escaping. During the egg protection period, the resistance of parent octopuses is weakened. Frequent changes in the environment should be avoided. The water temperature of the hatching pool should be kept at 24°C, and the light intensity should be adjusted to below 300Lx. Long running water should be used for cultivation to inhibit the growth of bacteria and other harmful organisms. And prevent the stimulation to the parent octopus when changing the water, improve the quality of egg protection. During the egg protection period, the parent octopus seldom takes the initiative to feed. Feed once every 2 to 3 days to maintain the physical fitness of the parent. When casting bait, remove the net that seals the octopus nest, cut off the claws of the fiddler crab or the crab near the meat ball, and throw it into the octopus nest, or remove half of the shell of the Philippine clam and four-horned clam and put it into the octopus nest. Feed one in the octopus nest, then seal the octopus nest with a net, put it back into the hatching pond, and clean up the residual bait the next day. During the period of pro-octopus egg protection, check the fertilized eggs every day, and remove the swollen bad eggs and dead eggs in time. With the egg protection device and method for long octopuses, the hatching rate can reach 90%, which is higher than the hatching rate of the general method. the
本发明中蛸巢结构根据自然界中长蛸巢穴设计,模拟自然产卵环境,有效地保护了亲体,防止了亲体间的争斗及残食,不仅提高了亲蛸的成活率,还提高了亲蛸的产卵量、受精卵的孵化率和幼体质量。亲蛸护卵期间活动较少,免疫力降低,采取主动在蛸巢中投饵的方法,使亲体通过摄食补充能量,采取降低光照强度、保持温度恒定、流水培养等方法保护亲蛸,提高护卵质量。 The structure of the octopus nest in the present invention is designed according to the nests of long octopuses in nature, simulating the natural spawning environment, effectively protecting the parents, preventing fights and cannibalism between the parents, not only improving the survival rate of the parent octopus, but also improving the survival rate of the parent octopus. The number of eggs laid, the hatching rate of fertilized eggs and the quality of larvae. During the egg protection period, the parent octopus is less active and the immunity is lowered. The method of actively feeding the octopus nest is adopted to make the parent body replenish energy through feeding. The parent octopus is protected by reducing the light intensity, keeping the temperature constant, and running water culture to improve the protection. egg quality. the
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