CN107439353A - A kind of tissue culture formula rhizoma Gastrodiae raising technology - Google Patents
A kind of tissue culture formula rhizoma Gastrodiae raising technology Download PDFInfo
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- CN107439353A CN107439353A CN201710817862.7A CN201710817862A CN107439353A CN 107439353 A CN107439353 A CN 107439353A CN 201710817862 A CN201710817862 A CN 201710817862A CN 107439353 A CN107439353 A CN 107439353A
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- rhizoma gastrodiae
- bacterium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C1/00—Ammonium nitrate fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention proposes a kind of tissue culture formula rhizoma Gastrodiae raising technology,Elm is chosen first,Chinese scholartree or birch interception branch,Branch is cut into by segment by cutter again,Bacterium branch is processed with this,Or by elm,Chinese scholartree or birch bar are sawn into the section of 1.0 1.5 chis length,The side of section is uniformly chopped to 4 row's fish scale mouths,At a distance of 2.0 3.0 cun between upper and lower fish scale mouth,Bacteria stick is intercepted with this,Bacterium branch and bacteria stick are soaked 12 hours using nutrient solution and taken out,The surface of bacterium branch and bacteria stick is air-dried again,Picking individual development is complete,Disease-free spot,Without deformity,Color and luster is normal,Eye substantially plants fiber crops,Bacterium material is soaked by nutrient solution in plantation,It can make it that the germination percentage of rhizoma Gastrodiae is higher with this,And nutrient solution is mixed into soil,So that the growth of rhizoma Gastrodiae is more preferable,Than and add nutrient solution in potato water,So that rhizoma Gastrodiae growth is more preferable,The tissue culture formula rhizoma Gastrodiae raising technology step is simple,And the Quality of Gastrodia Elata Bl bred is more consummate.
Description
Technical field
The present invention relates to rhizoma Gastrodiae raising technology field, specially a kind of tissue culture formula rhizoma Gastrodiae raising technology.
Background technology
Rhizoma Gastrodiae, also known as rhizoma gastrodiae, DINGFENGCAO, from mother, Bai Longpi, RHIZONA GASTRODIAE, be a kind of rare Chinese medicine.Rhizoma Gastrodiae nature and flavor
It is sweet, pungent, flat, nontoxic, return liver warp, energy Inflammation Zhijing, Pinggan Yang, dispelling wind and removing obstruction in the meridians, there is treatment to faint from fear, twitch contraction, dizziness, head
Bitterly, hemiplegia, extremity numbness, arthralgia pain due to rheumatism and other effects.Gastrodia orchid family perennial herb parasitic animal and plant, unrooted is without leaf, plant
In do not contain chlorophyll, thus photosynthesis can not be carried out and autotrophy, directly can not absorb nutrition from the external world, can only be with white mushroom
Section's halimasch symbiosis, provide growth required nutrient for rhizoma Gastrodiae by halimasch, Gastrodia protocorm is cultivated under usual manner
Not high with halimasch Percentage bound, easy infection bacterium or miscellaneous bacteria cause the underproduction or total crop failure;It is subject to the insect pest plague of rats and other animals danger
Evil;The seed cultivated is very high with residual risk in spite of illness, is easily caused the plantation underproduction or total crop failure of next step;Halimasch under usual manner
Nutrition is not sufficient enough in compost, the seed individual slight of stature of production, it has not been convenient to manage.
The content of the invention
The technical problem to be solved in the present invention is to overcome the defects of existing, there is provided a kind of tissue culture formula rhizoma Gastrodiae raising technology, can
Effectively to solve the problems, such as in background technology.
To achieve the above object, the present invention proposes:A kind of tissue culture formula rhizoma Gastrodiae raising technology, comprises the following steps:
S1)Bacterium material prepares:Elm, Chinese scholartree or birch interception branch are chosen first, then branch is cut into by segment by cutter, with
This is processed to bacterium branch, and elm, Chinese scholartree or birch bar are either sawn into the section of 1.0-1.5 chis length, and the side of section is uniform
It is chopped to 4 row's fish scale mouths, at a distance of 2.0-3.0 cuns between upper and lower fish scale mouth, bacteria stick is intercepted with this;
S2)The processing of bacterium material:Bacterium branch and bacteria stick immersion 1-2 hours are taken out using nutrient solution, then by bacterium branch and the surface wind of bacteria stick
It is dry;
S3)Seed selection:Picking individual development is complete, disease-free spot, without deformity, color and luster is normal, eye substantially plants fiber crops;
S4)Tissue culture arranges:Selection fills up tissue culture frame rich in the sand of organic humic, then by sand mix into
Row stirring, then flattens the sand on tissue culture frame;
S5)Plantation:Then in soil dig pit, then the bacterium branch and bacteria stick of preparation are placed into the hole dug, will plant
Fiber crops are put close to bacterium material, and six kind fiber crops are put on a root fungus material, four are placed among bacterium material, the both ends of bacterium material respectively place one
It is individual;
S6)Covering:Sawdust, straw, cogongrass or the equal big plantation of powder uniform fold of wheat bran are used on the soil planted
On good soil, the thickness of covering is 2.0-3.0, then is filled and led up by the soil of detritus, then small to being outputed around plantation hole again
Ditch;
S7)Management:In rhizoma Gastrodiae growth period so that the water content of soil is maintained at 25%-35%, is maintained at the humidity of air
55%-60%, air is maintained at 20-25 degree steadily, and when rhizoma Gastrodiae expands peak period, nutrient solution will be added in potato water,
Mixed water is sprayed by flusher to rhizoma Gastrodiae, after germination, every watering in 4-5 days once, during watering, passes through water
Pipe is watered in the ditch outputed, and periodically the air to interior is filtered, changed, and prevents disease pest from producing;
S8)Harvest:After soil first above plantation hole is removed, then starting bacterium material slowly, plucked to rhizoma Gastrodiae.
As a preferred technical solution of the present invention:When choosing bacterium branch, the length of bacterium branch is set to be maintained at 8-11 centimetres, and
And a diameter of 2-3 centimetres of the bacterium branch chosen.
As a preferred technical solution of the present invention:When digging pit, the depth in hole is set to remain to 20-25 centimetres, plantation
Before, by being watered by water pipe in the hole dug, wait water to be immersed in soil and planted again.
As a preferred technical solution of the present invention:The nutrient solution is prepared by clear water, ammonium nitrate and magnesium sulfate and completed, clearly
The quality proportioning of water, ammonium nitrate and magnesium sulfate is 100:1:1.
Compared with prior art, the beneficial effects of the invention are as follows:Bacterium material is soaked by nutrient solution in plantation, with
This can make it that the germination percentage of rhizoma Gastrodiae is higher, and nutrient solution has been mixed into soil so that the growth of rhizoma Gastrodiae is more preferable, than and
Nutrient solution is added in potato water so that rhizoma Gastrodiae growth is more preferable, and the tissue culture formula rhizoma Gastrodiae raising technology step is simple, and breed
Quality of Gastrodia Elata Bl is more consummate.
Embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described,
Obviously, described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, all
Belong to the scope of protection of the invention.
The present invention provides following technical scheme:
Embodiment one:
A kind of tissue culture formula rhizoma Gastrodiae raising technology, comprises the following steps:
S1)Bacterium material prepares:Elm, Chinese scholartree or birch interception branch are chosen first, then branch is cut into by segment by cutter, with
This is processed to bacterium branch, and elm, Chinese scholartree or birch bar are either sawn into the section of 1.0 chis length, the side of section is uniformly chopped into 4
Fish scale mouth is arranged, at a distance of 2.0 cun between upper and lower fish scale mouth, bacteria stick is intercepted with this, when choosing bacterium branch, is maintained at the length of bacterium branch
8 centimetres, and a diameter of 2 centimetres of the bacterium branch chosen;
S2)The processing of bacterium material:Bacterium branch and bacteria stick are soaked 1 hour using nutrient solution and taken out, then the surface of bacterium branch and bacteria stick is air-dried,
Nutrient solution is prepared by clear water, ammonium nitrate and magnesium sulfate and completed, and the quality proportioning of clear water, ammonium nitrate and magnesium sulfate is 100:1:1;
S3)Seed selection:Picking individual development is complete, disease-free spot, without deformity, color and luster is normal, eye substantially plants fiber crops;
S4)Tissue culture arranges:Sand of the selection rich in organic humic fills up tissue culture frame, then mixes sand and seeks
Nutrient solution is stirred, and then flattens the sand on tissue culture frame;
S5)Plantation:Then in soil dig pit, then the bacterium branch and bacteria stick of preparation are placed into the hole dug, will plant
Fiber crops are put close to bacterium material, and six kind fiber crops are put on a root fungus material, four are placed among bacterium material, the both ends of bacterium material respectively place one
It is individual, when digging pit, the depth in hole is remained to 20 centimetres, before plantation, by being watered by water pipe in the hole dug, wait water
It is immersed in soil and is planted again;
S6)Covering:Sawdust, straw, cogongrass or the equal big plantation of powder uniform fold of wheat bran are used on the soil planted
On good soil, the thickness of covering is 2.0 centimetres, then is filled and led up by the soil of detritus, then small to being outputed around plantation hole again
Ditch;
S7)Management:In rhizoma Gastrodiae growth period so that the water content of soil is maintained at 25%, the humidity of air is maintained at 55%, makes
Air is maintained at 20 degree steadily, and when rhizoma Gastrodiae expands peak period, nutrient solution will be added in potato water, mixed water passes through
Flusher sprays to rhizoma Gastrodiae, after germination, every watering in 4 days once, during watering, by water pipe in the ditch outputed
Watered, periodically the air to interior is filtered, changed, and prevents disease pest from producing;
S8)Harvest:After soil first above plantation hole is removed, then starting bacterium material slowly, plucked to rhizoma Gastrodiae.
Embodiment two:
A kind of tissue culture formula rhizoma Gastrodiae raising technology, comprises the following steps:
S1)Bacterium material prepares:Elm, Chinese scholartree or birch interception branch are chosen first, then branch is cut into by segment by cutter, with
This is processed to bacterium branch, and elm, Chinese scholartree or birch bar are either sawn into the section of 1.2 chis length, the side of section is uniformly chopped into 4
Fish scale mouth is arranged, at a distance of 2.3 cun between upper and lower fish scale mouth, bacteria stick is intercepted with this, when choosing bacterium branch, is maintained at the length of bacterium branch
9 centimetres, and a diameter of 1.2 centimetres of the bacterium branch chosen;
S2)The processing of bacterium material:Bacterium branch and bacteria stick are soaked 1.3 hours using nutrient solution and taken out, then by bacterium branch and the surface wind of bacteria stick
Dry, nutrient solution is prepared by clear water, ammonium nitrate and magnesium sulfate and completed, and the quality proportioning of clear water, ammonium nitrate and magnesium sulfate is 100:1:1;
S3)Seed selection:Picking individual development is complete, disease-free spot, without deformity, color and luster is normal, eye substantially plants fiber crops;
S4)Tissue culture arranges:Sand of the selection rich in organic humic fills up tissue culture frame, then mixes sand and seeks
Nutrient solution is stirred, and then flattens the sand on tissue culture frame;
S5)Plantation:Then in soil dig pit, then the bacterium branch and bacteria stick of preparation are placed into the hole dug, will plant
Fiber crops are put close to bacterium material, and six kind fiber crops are put on a root fungus material, four are placed among bacterium material, the both ends of bacterium material respectively place one
It is individual, when digging pit, the depth in hole is remained to 22 centimetres, before plantation, by being watered by water pipe in the hole dug, wait water
It is immersed in soil and is planted again;
S6)Covering:Sawdust, straw, cogongrass or the equal big plantation of powder uniform fold of wheat bran are used on the soil planted
On good soil, the thickness of covering is 2.3 centimetres, then is filled and led up by the soil of detritus, then small to being outputed around plantation hole again
Ditch;
S7)Management:In rhizoma Gastrodiae growth period so that the water content of soil is maintained at 28%, the humidity of air is maintained at 58%, makes
Air is maintained at 22 degree steadily, and when rhizoma Gastrodiae expands peak period, nutrient solution will be added in potato water, mixed water passes through
Flusher sprays to rhizoma Gastrodiae, after germination, every watering in 4 days once, during watering, by water pipe in the ditch outputed
Watered, periodically the air to interior is filtered, changed, and prevents disease pest from producing;
S8)Harvest:After soil first above plantation hole is removed, then starting bacterium material slowly, plucked to rhizoma Gastrodiae.
Embodiment three:
A kind of tissue culture formula rhizoma Gastrodiae raising technology, comprises the following steps:
S1)Bacterium material prepares:Elm, Chinese scholartree or birch interception branch are chosen first, then branch is cut into by segment by cutter, with
This is processed to bacterium branch, and elm, Chinese scholartree or birch bar are either sawn into the section of 1.4 chis length, the side of section is uniformly chopped into 4
Fish scale mouth is arranged, at a distance of 2.8 cun between upper and lower fish scale mouth, bacteria stick is intercepted with this, when choosing bacterium branch, is maintained at the length of bacterium branch
10 centimetres, and a diameter of 2.8 centimetres of the bacterium branch chosen;
S2)The processing of bacterium material:Bacterium branch and bacteria stick are soaked 1.8 hours using nutrient solution and taken out, then by bacterium branch and the surface wind of bacteria stick
Dry, nutrient solution is prepared by clear water, ammonium nitrate and magnesium sulfate and completed, and the quality proportioning of clear water, ammonium nitrate and magnesium sulfate is 100:1:1;
S3)Seed selection:Picking individual development is complete, disease-free spot, without deformity, color and luster is normal, eye substantially plants fiber crops;
S4)Tissue culture arranges:Sand of the selection rich in organic humic fills up tissue culture frame, then mixes sand and seeks
Nutrient solution is stirred, and then flattens the sand on tissue culture frame;
S5)Plantation:Then in soil dig pit, then the bacterium branch and bacteria stick of preparation are placed into the hole dug, will plant
Fiber crops are put close to bacterium material, and six kind fiber crops are put on a root fungus material, four are placed among bacterium material, the both ends of bacterium material respectively place one
It is individual, when digging pit, the depth in hole is remained between 24, before plantation, by being watered by water pipe in the hole dug, wait water
It is immersed in soil and is planted again;
S6)Covering:Sawdust, straw, cogongrass or the equal big plantation of powder uniform fold of wheat bran are used on the soil planted
On good soil, the thickness of covering is 2.8 centimetres, then is filled and led up by the soil of detritus, then small to being outputed around plantation hole again
Ditch;
S7)Management:In rhizoma Gastrodiae growth period so that the water content of soil is maintained at 32%, the humidity of air is maintained at 58%, makes
Air is maintained at 24 degree steadily, and when rhizoma Gastrodiae expands peak period, nutrient solution will be added in potato water, mixed water passes through
Flusher sprays to rhizoma Gastrodiae, after germination, every watering in 5 days once, during watering, by water pipe in the ditch outputed
Watered, periodically the air to interior is filtered, changed, and prevents disease pest from producing;
S8)Harvest:After soil first above plantation hole is removed, then starting bacterium material slowly, plucked to rhizoma Gastrodiae.
Example IV:
A kind of tissue culture formula rhizoma Gastrodiae raising technology, comprises the following steps:
S1)Bacterium material prepares:Elm, Chinese scholartree or birch interception branch are chosen first, then branch is cut into by segment by cutter, with
This is processed to bacterium branch, and elm, Chinese scholartree or birch bar are either sawn into the section of 1.5 chis length, the side of section is uniformly chopped into 4
Fish scale mouth is arranged, at a distance of 3.0 cun between upper and lower fish scale mouth, bacteria stick is intercepted with this, when choosing bacterium branch, is maintained at the length of bacterium branch
11 centimetres, and a diameter of 3 centimetres of the bacterium branch chosen;
S2)The processing of bacterium material:Bacterium branch and bacteria stick are soaked 2 hours using nutrient solution and taken out, then the surface of bacterium branch and bacteria stick is air-dried,
Nutrient solution is prepared by clear water, ammonium nitrate and magnesium sulfate and completed, and the quality proportioning of clear water, ammonium nitrate and magnesium sulfate is 100:1:1;
S3)Seed selection:Picking individual development is complete, disease-free spot, without deformity, color and luster is normal, eye substantially plants fiber crops;
S4)Tissue culture arranges:Sand of the selection rich in organic humic fills up tissue culture frame, then mixes sand and seeks
Nutrient solution is stirred, and then flattens the sand on tissue culture frame;
S5)Plantation:Then in soil dig pit, then the bacterium branch and bacteria stick of preparation are placed into the hole dug, will plant
Fiber crops are put close to bacterium material, and six kind fiber crops are put on a root fungus material, four are placed among bacterium material, the both ends of bacterium material respectively place one
It is individual, when digging pit, the depth in hole is remained between 25, before plantation, by being watered by water pipe in the hole dug, wait water
It is immersed in soil and is planted again;
S6)Covering:Sawdust, straw, cogongrass or the equal big plantation of powder uniform fold of wheat bran are used on the soil planted
On good soil, the thickness of covering is 3.0 centimetres, then is filled and led up by the soil of detritus, then small to being outputed around plantation hole again
Ditch;
S7)Management:In rhizoma Gastrodiae growth period so that the water content of soil is maintained at 35%, the humidity of air is maintained at 60%, makes
Air is maintained at 25 degree steadily, and when rhizoma Gastrodiae expands peak period, nutrient solution will be added in potato water, mixed water passes through
Flusher sprays to rhizoma Gastrodiae, after germination, every watering in 5 days once, during watering, by water pipe in the ditch outputed
Watered, periodically the air to interior is filtered, changed, and prevents disease pest from producing;
S8)Harvest:After soil first above plantation hole is removed, then starting bacterium material slowly, plucked to rhizoma Gastrodiae.
Benefit of the present invention:Bacterium material is soaked by nutrient solution in plantation, the germination percentage of rhizoma Gastrodiae can be caused with this
It is higher, and nutrient solution has been mixed into soil so that the growth of rhizoma Gastrodiae is more preferable, than and add nutrient solution in potato water, makes
Obtain rhizoma Gastrodiae growth more preferably, the tissue culture formula rhizoma Gastrodiae raising technology step is simple, and the Quality of Gastrodia Elata Bl bred is more consummate
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, it is possible to understand that
These embodiments can be carried out with a variety of changes, modification without departing from the principles and spirit of the present invention, replace and become
Type, the scope of the present invention is defined by the appended.
Claims (4)
- A kind of 1. tissue culture formula rhizoma Gastrodiae raising technology, it is characterised in that:Comprise the following steps:S1)Bacterium material prepares:Elm, Chinese scholartree or birch interception branch are chosen first, then branch is cut into by segment by cutter, with This is processed to bacterium branch, and elm, Chinese scholartree or birch bar are either sawn into the section of 1.0-1.5 chis length, and the side of section is uniform It is chopped to 4 row's fish scale mouths, at a distance of 2.0-3.0 cuns between upper and lower fish scale mouth, bacteria stick is intercepted with this;S2)The processing of bacterium material:Bacterium branch and bacteria stick immersion 1-2 hours are taken out using nutrient solution, then by bacterium branch and the surface wind of bacteria stick It is dry;S3)Seed selection:Picking individual development is complete, disease-free spot, without deformity, color and luster is normal, eye substantially plants fiber crops;S4)Tissue culture arranges:Sand of the selection rich in organic humic fills up tissue culture frame, then mixes sand and seeks Nutrient solution is stirred, and then flattens the sand on tissue culture frame;S5)Plantation:Then in soil dig pit, then the bacterium branch and bacteria stick of preparation are placed into the hole dug, will plant Fiber crops are put close to bacterium material, and six kind fiber crops are put on a root fungus material, four are placed among bacterium material, the both ends of bacterium material respectively place one It is individual;S6)Covering:Sawdust, straw, cogongrass or the equal big plantation of powder uniform fold of wheat bran are used on the soil planted On good soil, the thickness of covering is 2.0-3.0 centimetres, then is filled and led up by the soil of detritus, then again to being opened around plantation hole Go out ditch;S7)Management:In rhizoma Gastrodiae growth period so that the water content of soil is maintained at 25%-35%, is maintained at the humidity of air 55%-60%, air is maintained at 20-25 degree steadily, and when rhizoma Gastrodiae expands peak period, nutrient solution will be added in potato water, Mixed water is sprayed by flusher to rhizoma Gastrodiae, after germination, every watering in 4-5 days once, during watering, passes through water Pipe is watered in the ditch outputed, and periodically the air to interior is filtered, changed, and prevents disease pest from producing;S8)Harvest:After soil first above plantation hole is removed, then starting bacterium material slowly, plucked to rhizoma Gastrodiae.
- 2. a kind of tissue culture formula rhizoma Gastrodiae raising technology according to claim 1, it is characterised in that when choosing bacterium branch, make bacterium branch Length is maintained at 8-11 centimetres, and a diameter of 2-3 centimetres of the bacterium branch chosen.
- 3. a kind of tissue culture formula rhizoma Gastrodiae raising technology according to claim 1, it is characterised in that when digging pit, make the depth in hole 20-25 centimetres, before plantation is remained to, by being watered by water pipe in the hole dug, waits water to be immersed in soil and carries out again Plantation.
- 4. a kind of tissue culture formula rhizoma Gastrodiae raising technology according to claim 1, it is characterised in that the nutrient solution is by clear water, nitre Ammonium and magnesium sulfate, which are prepared, to be completed, and the quality proportioning of clear water, ammonium nitrate and magnesium sulfate is 100:1:1.
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CN108934810B (en) * | 2018-06-29 | 2020-12-01 | 安徽德昌药业股份有限公司 | Three-dimensional gastrodia elata cultivation device and gastrodia elata cultivation method thereof |
CN114208616A (en) * | 2021-12-31 | 2022-03-22 | 西南林业大学 | Organic planting method for gastrodia elata |
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