CN107429254B - 原代造血细胞中的蛋白递送 - Google Patents
原代造血细胞中的蛋白递送 Download PDFInfo
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- CN107429254B CN107429254B CN201680016762.8A CN201680016762A CN107429254B CN 107429254 B CN107429254 B CN 107429254B CN 201680016762 A CN201680016762 A CN 201680016762A CN 107429254 B CN107429254 B CN 107429254B
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- C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
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- C12N15/90—Stable introduction of foreign DNA into chromosome
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- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562110187P | 2015-01-30 | 2015-01-30 | |
| US62/110,187 | 2015-01-30 | ||
| US201562209711P | 2015-08-25 | 2015-08-25 | |
| US62/209,711 | 2015-08-25 | ||
| PCT/US2016/015836 WO2016123578A1 (en) | 2015-01-30 | 2016-01-29 | Protein delivery in primary hematopoietic cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107429254A CN107429254A (zh) | 2017-12-01 |
| CN107429254B true CN107429254B (zh) | 2021-10-15 |
Family
ID=56544445
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201680016762.8A Active CN107429254B (zh) | 2015-01-30 | 2016-01-29 | 原代造血细胞中的蛋白递送 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20190388469A1 (https=) |
| EP (2) | EP3929296A1 (https=) |
| JP (3) | JP6886404B2 (https=) |
| KR (1) | KR102605464B1 (https=) |
| CN (1) | CN107429254B (https=) |
| AU (2) | AU2016211161C1 (https=) |
| ES (1) | ES2880473T5 (https=) |
| IL (1) | IL253498B (https=) |
| SG (1) | SG11201706059SA (https=) |
| WO (1) | WO2016123578A1 (https=) |
Families Citing this family (91)
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| JP6261500B2 (ja) | 2011-07-22 | 2018-01-17 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ヌクレアーゼ切断特異性の評価および改善 |
| EP3597741A1 (en) | 2012-04-27 | 2020-01-22 | Duke University | Genetic correction of mutated genes |
| AU2014227653B2 (en) | 2013-03-15 | 2017-04-20 | The General Hospital Corporation | Using RNA-guided foki nucleases (RFNs) to increase specificity for RNA-guided genome editing |
| US9828582B2 (en) | 2013-03-19 | 2017-11-28 | Duke University | Compositions and methods for the induction and tuning of gene expression |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| US20200332259A1 (en) * | 2014-11-04 | 2020-10-22 | Versiti Blood Research Institute Foundation, Inc. | Method to bioengineer designer red blood cells using gene editing and stem cell methodologies |
| US10676726B2 (en) | 2015-02-09 | 2020-06-09 | Duke University | Compositions and methods for epigenome editing |
| AU2016261358B2 (en) | 2015-05-11 | 2021-09-16 | Editas Medicine, Inc. | Optimized CRISPR/Cas9 systems and methods for gene editing in stem cells |
| EP3307887A1 (en) | 2015-06-09 | 2018-04-18 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for improving transplantation |
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| US11643668B2 (en) | 2015-06-17 | 2023-05-09 | The Uab Research Foundation | CRISPR/Cas9 complex for genomic editing |
| GB2592821B (en) | 2015-07-31 | 2022-01-12 | Univ Minnesota | Modified cells and methods of therapy |
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