CN107402269B - 基于scx/sax混合填料的集成化蛋白质组学样品前处理平台及其应用 - Google Patents
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/08—Preparation using an enricher
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- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
Description
比例 | 吸附容量(μg) |
5:1 | 148±10 |
3:1 | 156±10 |
1:1 | 182±10 |
1:3 | 146±10 |
1:5 | 131±10 |
7:3 | 126±10 |
10:1 | 116±10 |
1:10 | 113±10 |
12:1 | 85±10 |
1:12 | 83±10 |
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CN108593818B (zh) * | 2018-05-08 | 2021-03-16 | 重庆市食品药品检验检测研究院 | 一种测定尼麦角林有关物质的高效液相色谱方法 |
CN109061016A (zh) * | 2018-07-25 | 2018-12-21 | 大连工业大学 | 一种富集生物胺的固相萃取柱的制备方法与应用 |
CN111208243B (zh) * | 2018-11-21 | 2022-05-17 | 中国科学院大连化学物理研究所 | 一种基于阴离子交换色谱柱的sumo化肽段的富集方法 |
CN111487349A (zh) * | 2020-05-15 | 2020-08-04 | 易达斯生物科技(苏州)有限公司 | 用于选择性富集纯化皂苷的色谱柱及其使用方法 |
CN111766325B (zh) * | 2020-07-20 | 2022-09-20 | 南方科技大学 | 一种多组学分析的样品前处理方法及其应用 |
CN112649490A (zh) * | 2020-12-09 | 2021-04-13 | 复旦大学 | 一种一体化快速蛋白酶解方法 |
CN113267554A (zh) * | 2021-05-14 | 2021-08-17 | 谱天(天津)生物科技有限公司 | 一种用于评估蛋白质组还原烷基化效率的质控方法 |
CN114509521B (zh) * | 2022-01-19 | 2023-12-19 | 南方科技大学 | 一种糖蛋白质组学样品前处理装置及方法 |
CN115184526A (zh) * | 2022-05-31 | 2022-10-14 | 南方科技大学 | 一种基于混合模式填料的少量细胞蛋白质组学反应器及其应用 |
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CN102133488A (zh) * | 2010-01-26 | 2011-07-27 | 上海安谱科学仪器有限公司 | 同时萃取三聚氰胺和三聚氰酸的固相萃取小柱及其制备方法和应用 |
CN105866317A (zh) * | 2016-03-31 | 2016-08-17 | 南方科技大学 | 集成蛋白质前处理和多肽高pH值反相分级的蛋白质组反应器及其应用 |
CN106770814A (zh) * | 2016-11-18 | 2017-05-31 | 南方科技大学 | 一种蛋白质色谱分离平台及其应用 |
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CN102133488A (zh) * | 2010-01-26 | 2011-07-27 | 上海安谱科学仪器有限公司 | 同时萃取三聚氰胺和三聚氰酸的固相萃取小柱及其制备方法和应用 |
CN105866317A (zh) * | 2016-03-31 | 2016-08-17 | 南方科技大学 | 集成蛋白质前处理和多肽高pH值反相分级的蛋白质组反应器及其应用 |
CN106770814A (zh) * | 2016-11-18 | 2017-05-31 | 南方科技大学 | 一种蛋白质色谱分离平台及其应用 |
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