CN107396861A - A kind of method of the more squama shovel jaw fish artificial induced spawnings in southwest - Google Patents
A kind of method of the more squama shovel jaw fish artificial induced spawnings in southwest Download PDFInfo
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- CN107396861A CN107396861A CN201710713035.3A CN201710713035A CN107396861A CN 107396861 A CN107396861 A CN 107396861A CN 201710713035 A CN201710713035 A CN 201710713035A CN 107396861 A CN107396861 A CN 107396861A
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000002347 injection Methods 0.000 claims abstract description 55
- 239000007924 injection Substances 0.000 claims abstract description 55
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 210000001015 abdomen Anatomy 0.000 claims description 12
- 230000002196 ecbolic effect Effects 0.000 claims description 10
- 239000005556 hormone Substances 0.000 claims description 10
- 229940088597 hormone Drugs 0.000 claims description 10
- 210000004907 gland Anatomy 0.000 claims description 7
- 230000001568 sexual effect Effects 0.000 claims description 7
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 6
- 239000003814 drug Substances 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 4
- 241000252233 Cyprinus carpio Species 0.000 claims description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 3
- 230000026109 gonad development Effects 0.000 claims description 3
- 229910052742 iron Inorganic materials 0.000 claims description 3
- 210000000006 pectoral fin Anatomy 0.000 claims description 3
- 239000003488 releasing hormone Substances 0.000 claims description 3
- 210000000582 semen Anatomy 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 230000000638 stimulation Effects 0.000 claims description 3
- 230000018109 developmental process Effects 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 230000001817 pituitary effect Effects 0.000 claims 1
- 230000032696 parturition Effects 0.000 abstract description 7
- 230000004720 fertilization Effects 0.000 abstract description 4
- 238000011534 incubation Methods 0.000 abstract description 4
- 238000009313 farming Methods 0.000 abstract description 3
- 230000000384 rearing effect Effects 0.000 abstract description 3
- 241000404692 Opistognathidae Species 0.000 description 6
- 210000004556 brain Anatomy 0.000 description 2
- 210000002149 gonad Anatomy 0.000 description 2
- 241000219307 Atriplex rosea Species 0.000 description 1
- 241000251730 Chondrichthyes Species 0.000 description 1
- 241000252210 Cyprinidae Species 0.000 description 1
- 241000252206 Cypriniformes Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001505014 Onychostoma macrolepis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/60—Fish, e.g. seahorses; Fish eggs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/08—Peptides having 5 to 11 amino acids
- A61K38/09—Luteinising hormone-releasing hormone [LHRH], i.e. Gonadotropin-releasing hormone [GnRH]; Related peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/24—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Zoology (AREA)
- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Endocrinology (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Reproductive Health (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Wood Science & Technology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of method of the more squama shovel jaw fish artificial induced spawnings in southwest, comprise the following steps：(1) parent population is selected：It is the Best Times of artificial induced spawning before and after annual Clear and Bright；(2) flowing water pond deposit parent population；(3) inject for the first time：Raun injection dosage is：First time injection dosage is that every kilogram of parent population injects the μ g of LRH A 4, and milter injection dosage is the 1/2 of raun；(4) second of injection：Carry out injecting for second after 24 hours, raun injection dosage is：Every kilogram of μ g+HCG 800IU+PG 35 of parent population injection LRH A 4, LRH A2 800IU/kg, milter injection dosage is the 1/2 of raun.The present invention solves the key technology difficulty of more squama shovel jaw fish artificial propagations, and then realizes that seed rearing and marketable fish artificial farming provide safeguard, and is commercialized on the premise of realizing more squama shovel jaw fish species conservations.After being hastened parturition using this method, male and female synchronization maturity, spawning rate, rate of fertilization and incubation rate are up to more than 90%.
Description
Technical field
Hastened parturition field the present invention relates to Technique in Fishes, the side of specifically a kind of more squamas shovel jaw fish artificial induced spawnings in southwest
Method.
Background technology
More squama shovel jaw fishes (Varicorhinus macrolepis), category Cypriniformes, Cyprinidae, Barbinae, prominent kiss fish category, custom
Claim money fish, the white first, red scale fish of the tip, stone mouth fish, inhabit in gravel substrate, the clear temperature of water is low, flow velocity is larger, 300-1500 meters of height above sea level
Mountain stream water small stream.Southwest is mainly distributed on Daning River upstream waters, and individual is than the tai shan area big (In The Taishan Area of individual
20 centimetres are usually no more than into fish length, body weight is no more than 100 grams), it is maximum up to more than 1 kilogram.It is reported that more squama shovel jaw fishes
Fine and tender taste, nutritious, medicinal health value is high, its DHA, EPA content are respectively 6.20%, 2.89%, are shark respectively
48 times and 32 times, but at present its wild resource it is very deficient.Before the artificial propagation that this project team carries out more squama shovel jaw fishes,
Southwest has no successfully to be reported on the shovel jaw fish artificial propagation of more squamas.The districts such as southwest Chengkou County, Wuxi County have few
Number plant stores a number of more squama shovel jaw fish (entrance of the city is referred to as Cheng Kou Lian fishes, Wuxi is referred to as money fish) parent populations, supports
The artificial propagation trial of more squama shovel jaw fishes can all be carried out every year by growing field, but not succeeded.When main cause is artificial induced spawning
Between and method it is improper, rate of fertilization is low and open-mouthed bait does not solve well.Traditional artificial propagation of fish is all higher in water temperature
Shi Jinhang, corresponding Effect time are shorter.More squama shovel jaw fishes are distributed in the flowing water of mountain area more, and repoductive time is more early, and water temperature is relatively low, effect
It is longer between seasonable, the problems such as male and female are ripe asynchronous, it is artificial induced spawning side the shortcomings that current more squamas shovel jaw fish artificial propagation maximums
Method and time of hastening parturition are improper.Therefore need solve the problems, such as that more squama shovel jaw fish artificial propagation induced spawning methods are improper.
The content of the invention
It is an object of the invention to provide a kind of method of the more squama shovel jaw fish artificial induced spawnings in southwest, to solve the above-mentioned back of the body
The problem of being proposed in scape technology, more squama shovel jaw fish artificial induced spawning success rates are improved, and then improve fertility rate and hatchability.
To achieve the above object, the present invention provides following technical scheme：
A kind of method of the more squama shovel jaw fish artificial induced spawnings in southwest, comprises the following steps：
(1) parent population is selected：Before and after annual clear and bright, the situation that milter pursues and attacks raun is had in reserve parent fish great Chi, is artificial
The Best Times hastened parturition；
(2) flowing water pond deposit parent population：The healthy and strong fish fully ripe without wound, sexual gland of selection is as male and female parent population, according to hero
The scattered degree of fish seminal concentration, chance moisture and raun belly size, soft degree differentiate its sexal maturity situation, select parent population and are put into
Rectangle gutter channel, and suitably regulation flowing water intensity is stimulated, to promote gonad development；
(3) inject for the first time：To the parent population chosen, manual injection's oxytocic hormone is carried out, oxytocic hormone hangs down for carp brain
Body PG, luteotropin releasing hormone d-ala analog LRH-A and human chorionic gonadtropin HCG；Double injection method is sampled to carry out manually
Injection of hormone, the time interval of pin injection twice is 24 hours, and all solids oxytocic hormone uses normal saline dilution, raun
Injection dosage is：First time injection dosage is that every kilogram of parent population injects the μ g of LRH-A 4, and milter injection dosage is the 1/2 of raun；
(4) second of injection：Carry out injecting for second after 24 hours, raun injection dosage is：Every kilogram of parent population injection
The μ g+HCG 800IU+PG 3-5 grains of LRH-A 4, LRH-A2800IU/kg, milter injection dosage are the 1/2 of raun；Injection is hastened parturition
Male and female parent population is separately put into the gutter channel separated with iron bar after medicine, micro- stimulation by running water, and checks parent population every 4 hours
Developmental state.
As the further scheme of the present invention：The fully ripe raun belly of sexual gland substantially expands in step (2), feel
Soft, flexible, gonopore is ruddy, and the fully ripe milter rhynchodaenm cone astropyle of sexual gland goes out, and the belly raun that compares is small, light pressure
Belly can flow out milky seminal fluid, and can be scattered rapidly in water.
As further scheme of the invention：The specification of gutter channel described in step (2) is 15m × 0.6m × 0.8m,
Water inlet flow velocity is 0.3-0.5m3/s。
As further scheme of the invention：The parent population chosen in step (3) is injected using pectoral fin base portion, and depth is
5mm。
As further scheme of the invention：Second of injection dosage of raun is in step (4)：Every kilogram of parent population injection
LRH-A 4 μ g+HCG 800IU+PG 4, LRH-A2800IU/kg.
As further scheme of the invention：Due to by parent population maturity, water temperature and injection needle time difference in step (4)
And the influence of injection dosage, more pin time injecting effect times of squama shovel jaw fish two are 46-50h.
Compared with prior art, the beneficial effects of the invention are as follows：The present invention carries out manual injection using double injection method,
It it is 24 hours every the time.So as to solve the key technology difficulty of more squama shovel jaw fish artificial propagations, and then realize seed rearing and business
The artificial farming of product fish provides safeguard, and is commercialized on the premise of realizing more squama shovel jaw fish species conservations.Urged using this method
In postpartum, male and female synchronization maturity, spawning rate, rate of fertilization and incubation rate are up to more than 90%.
Brief description of the drawings
Accompanying drawing described herein is used for providing a further understanding of the present invention, forms the part of the present invention, this hair
Bright schematic description and description is used to explain the present invention, does not form inappropriate limitation of the present invention.In the accompanying drawings：
Fig. 1 is the flow chart for the method that the more squamas in southwest shovel jaw fish artificial induced spawning.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made
Embodiment, belong to the scope of protection of the invention.
Referring to Fig. 1, in the embodiment of the present invention, a kind of method of the more squama shovel jaw fish artificial induced spawnings in southwest, including with
Lower step：
(1) parent population is selected：Before and after annual clear and bright, the situation that milter pursues and attacks raun is had in reserve parent fish great Chi, this when
Between be artificial induced spawning Best Times；
(2) flowing water pond deposit parent population：The healthy and strong fish fully ripe without wound, sexual gland of selection is as male and female parent population, according to hero
The scattered degree of fish seminal concentration, chance moisture and raun belly size, soft degree differentiate its sexal maturity situation, select parent population and are put into
Rectangle gutter channel (postpartum can do incubation tank), and suitably regulation flowing water intensity is stimulated, to promote gonad development；Gutter channel
Specification be 15m × 0.6m × 0.8m, water inlet flow velocity is 0.3-0.5m3/s；The visible belly of the good raun of maturity of fish gonads
Substantially expand, sponginess is flexible, and gonopore is ruddy, and the good milter rhynchodaenm cone astropyle of maturity of fish gonads goes out, and belly is relative
It is smaller than raun, it is light to press belly to flow out milky seminal fluid, and can be scattered rapidly in water；
(3) inject for the first time：To the parent population chosen, manual injection's oxytocic drug is carried out, oxytocic hormone hangs down for carp brain
Body (PG), luteotropin releasing hormone d-ala analog (LRH-A) and human chorionic gonadtropin (HCG)；Sampling double injection method is entered
Pedestrian's work injection of hormone, the time interval of pin injection twice is 24 hours, and all parent populations are injected using pectoral fin base portion, and depth is
5mm, all solids oxytocic drug use normal saline dilution, and raun injection dosage is：First time injection dosage is every kilogram
Parent population injects the μ g of LRH-A 4, and milter injection dosage is the 1/2 of raun；
(4) second of injection：Carry out injecting for second after 24 hours, raun injection dosage is：Every kilogram of parent population injection
The μ g+HCG 800IU+PG 3-5 grains of LRH-A 4, LRH-A2800IU/kg, milter injection dosage are the 1/2 of raun；Injection is hastened parturition
Male and female parent population is separately put into the gutter channel separated with iron bar after medicine, micro- stimulation by running water, and checks parent population every 4 hours
Developmental state；Due to being influenceed by parent population maturity, water temperature and injection needle time different and injection dosage, more pins of squama shovel jaw fish two
The injecting effect time is 46-50h.
The present invention carries out manual injection using double injection method, and interval time is 24 hours.So as to solve more squama shovel jaw fishes
The key technology difficulty of artificial propagation, and then realize that seed rearing and marketable fish artificial farming provide safeguard, realize more squama shovel jaws
Commercialized on the premise of fish species conservation.After being hastened parturition using this method, male and female synchronization maturity, spawning rate, rate of fertilization
And incubation rate is up to more than 90%.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.Any reference in claim should not be considered as to the involved claim of limitation.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It is appreciated that other embodiment.
Claims (6)
- A kind of 1. method of the more squama shovel jaw fish artificial induced spawnings in southwest, it is characterised in that comprise the following steps：(1) parent population is selected：Before and after annual clear and bright, the situation that milter pursues and attacks raun is had in reserve parent fish great Chi, is artificial induced spawning Best Times；(2) flowing water pond deposit parent population：The healthy and strong fish fully ripe without wound, sexual gland of selection is as male and female parent population, according to milter essence Liquid concentration, meet the scattered degree of moisture and raun belly size, soft degree differentiate its sexal maturity situation, select parent population be put into it is rectangular Shape gutter channel, and suitably regulation flowing water intensity is stimulated, to promote gonad development；(3) inject for the first time：To the parent population chosen, carry out manual injection's oxytocic hormone, oxytocic hormone be carp pituitary PG, Luteotropin releasing hormone d-ala analog LRH-A and human chorionic gonadtropin HCG；Sample double injection method and carry out artificial hormone note Penetrate, the time interval of pin injection twice is 24 hours, and all solids oxytocic hormone uses normal saline dilution, raun injection Measure and be：First time injection dosage is that every kilogram of parent population injects the μ g of LRH-A 4, and milter injection dosage is the 1/2 of raun；(4) second of injection：Carry out injecting for second after 24 hours, raun injection dosage is：Every kilogram of parent population injects LRH-A 4 μ g+HCG 800IU+PG 3-5 grains, LRH-A2 800IU/kg, milter injection dosage are the 1/2 of raun；After injecting oxytocic drug Male and female parent population is separately put into the gutter channel separated with iron bar, micro- stimulation by running water, and every 4 hours development feelings for checking parent population Condition.
- 2. the method for the more squama shovel jaw fish artificial induced spawnings in southwest according to claim 1, it is characterised in that step (2) The fully ripe raun belly of middle sexual gland substantially expands, and sponginess is flexible, and gonopore is ruddy, and sexual gland is fully ripe Milter rhynchodaenm cone astropyle go out, the belly raun that compares is small, light to press belly to flow out milky seminal fluid, and can be dissipated rapidly in water Open.
- 3. the method for the more squama shovel jaw fish artificial induced spawnings in southwest according to claim 1, it is characterised in that step (2) Described in the specification of gutter channel be 15m × 0.6m × 0.8m, water inlet flow velocity is 0.3-0.5m3/s.
- 4. the method for the more squama shovel jaw fish artificial induced spawnings in southwest according to claim 1, it is characterised in that step (3) In the parent population that chooses injected using pectoral fin base portion, depth 5mm.
- 5. the method for the more squama shovel jaw fish artificial induced spawnings in southwest according to claim 1, it is characterised in that step (4) Middle second of injection dosage of raun be：Every kilogram of parent population injection LRH-A 4 μ g+HCG 800IU+PG 4, LRH-A2 800IU/ kg。
- 6. the method for the more squama shovel jaw fish artificial induced spawnings in southwest according to claim 1, it is characterised in that step (4) In due to being influenceed by parent population maturity, water temperature and injection needle time different and injection dosage, more pins time injection effects of squamas shovel jaw fish two It is 46-50h between seasonable.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710713035.3A CN107396861A (en) | 2017-08-18 | 2017-08-18 | A kind of method of the more squama shovel jaw fish artificial induced spawnings in southwest |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710713035.3A CN107396861A (en) | 2017-08-18 | 2017-08-18 | A kind of method of the more squama shovel jaw fish artificial induced spawnings in southwest |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113925958A (en) * | 2021-09-30 | 2022-01-14 | 南方海洋科学与工程广东省实验室(湛江) | Oxytocic for Seriola quinqueradiata and oxytocic method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113925958A (en) * | 2021-09-30 | 2022-01-14 | 南方海洋科学与工程广东省实验室(湛江) | Oxytocic for Seriola quinqueradiata and oxytocic method |
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