CN107333982A - A kind of method for making matrix Bei Lu feeds with soya bean straw using Lenlinus edodes - Google Patents
A kind of method for making matrix Bei Lu feeds with soya bean straw using Lenlinus edodes Download PDFInfo
- Publication number
- CN107333982A CN107333982A CN201710727297.5A CN201710727297A CN107333982A CN 107333982 A CN107333982 A CN 107333982A CN 201710727297 A CN201710727297 A CN 201710727297A CN 107333982 A CN107333982 A CN 107333982A
- Authority
- CN
- China
- Prior art keywords
- grams
- culture
- feeds
- soya bean
- bean straw
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
Abstract
The present invention provide it is a kind of the method that matrix prepares Ke Li Lu feeds is made with soya bean straw using Lenlinus edodes, belong to field of feed, including the shake-flask seed cultures of Lenlinus edodes, seed tank culture, soya bean straw handle, connect the steps such as bacterium after fermentation culture, pelletizing forming.Research Lenlinus edodes of the invention main make the method technique that matrix prepares Ke Li Lu feeds with soya bean straw, and do matrix with soya bean straw, using modern technology, pass through the fermentation of Lenlinus edodes, the high density granular Zhuan Lu feeds formed by HTHP rolling, act not only as Shang Pin Lu feeds to be transported, and prevention winter drought, sick calamity, snow disaster are acquired a special sense.And nutrition is high, palatability is good, and digestibility is high, can turn into good biological feedstuff, feeding safe ready, moreover it is possible to which disease-resistant growth-promoting etc. has obvious economic benefits, also has obvious ecology and environmental protection effect.
Description
Technical field
The invention belongs to field of feed, especially relates to one kind and does matrix preparation using Lenlinus edodes with soya bean straw
The method of Li Lu feeds.
Background technology
Soybean stalks feed resource is wide, quantity is big, and soybean stalks contain cellulose, hemicellulose and pentosan, by means of
The fermentation of rumen microorganism, can be by digestibility and utilizations such as Niu Yang Lu.Utilization power ratio of the external every Western Europe country to soybean stalk
Preferably, about 40% soybean stalk is used as the mixed feed of ox, sheep.But soybean stalks are directly prepared into Niu Yang Lu and raised
Material, there is the defects of quality is thick and stiff, palatability is poor, absorption difference, low nutritive value.
Therefore it provides a kind of effect is good, cost is low, the soybean stalks processing method of suitable for China, it is directed to improving soya bean
Stalk palatability, digestibility is improved, have additional nutrients value, improves enzymolysis ability, makes full use of this resource, development grain-saving type poultry
Animal husbandry, it is that the present invention will complete big task.
The content of the invention
The technical problems to be solved by the invention are：One kind is provided and prepares Ke Li Lu feeds using soya bean straw using Lenlinus edodes
Method, for the drawbacks such as unstable, poor taste, not comprehensive nutrition, trophic disturbance that solve current Shi Lu feed efficiencies
Technical problem.
One kind of the present invention does matrix with soya bean straw using Lenlinus edodes and prepares Ke Li Lu feeds, it is characterized in that：Including
Following steps.
1st, mushroom inclined-plane parent species are chosen and carry out shake-flask seed culture；
2nd, seed tank culture：Inocalation method is protected using flame, by step 1）Shake-flask seed carry out seed tank culture it is standby.
3rd, weigh in proportion 77 parts of soya bean straw, 10 parts of wheat bran, 3 parts of maize flour, 6 parts of beancake powder, 1 part of gypsum, 1 part of white sugar,
Bacterium bag, each vaccination of inoculum concentration is made in 0.8 part of salt, 1.2 parts of spices that stir of calcium superphosphate, sterilizing, cooling, inoculation
20ml~30ml；
4th, bacterium culture is educated：
15 days interior room temperature control systems are at 26 DEG C ~ 28 DEG C after inoculation；15 ~ 30 days temperature controls are at 22 DEG C ± 2 DEG C；20 DEG C of temperature after 30 days
±2℃；Relative air humidity is controlled 45~60%；Bacterium germination room lucifuge；Cultivating in a fungus bag terminates；
5th, it is granulated：Take out step 4）Bacterium material in bacterium bag, the particle manufacture craft system through routine is into Lu feeds.
The purpose of raising deer is that requirement has higher reproductive capacity, the constitution of unsoundness, excellent production performance, these all with
Feeding management also has extremely close relation.The ectoenzyme that " mushroom mycelium " of the present invention is secreted in soya bean straw matrix
Such as laccase, manganese peroxidase, cellulase, zytase, can be with degraded cellulose, hemicellulose and lignin.Soya bean
Straw contain higher protein, digestible energy：0.71 million Jiao/kilogram；Crude protein：8.9%；Crude fibre：39.8%；Calcium：0.87% mass
Preferably.Soya bean straw feed one is can directly to save substantial amounts of concentrated feed grain, and hundred jin of soya bean straws can replace 3 kilograms of grains.Second,
Lu feeding back to the field adds the ability of soil.Third, soybean stalks feed can reduce the biological loss of energy, prevent burning from causing
Environmental pollution and protein resource loss.Fourth, solve the problems, such as that stable breeding improves livestock on hand rate.Fifth, soya bean straw feed really reaches
The purpose of soybean " eating dry squeeze only " is arrived.
The positive effect of the present invention is：
The method technique of a matrix preparation grain Lu feed is done with soya bean straw using Lenlinus edodes, and is main matrix with soya bean straw, is led to
The fermentation of Lenlinus edodes is crossed, the high density granular shape feed formed by HTHP rolling, acts not only as Shang Pin Lu feeds
Transported, and prevention winter drought and sick calamity, snow disaster are acquired a special sense.The side that the present invention is fermented using Liquid solid Bonding
Formula, accelerated 15 days than conventional method.Shiitake mushroom hypha has high protein, its lentinan contained, vitamin D Deng Dui Lu lifes
Long hair gives birth to good effect, and with nutrition height, palatability is good, digestibility is high, can turn into good biological feedstuff, feeding safety
It is convenient, moreover it is possible to disease-resistant growth-promoting etc..
Embodiment
By following examples further illustrate description the present invention, do not limit the invention in any way, without departing substantially from
On the premise of the technical solution of the present invention, what those of ordinary skill in the art made for the present invention easily realized any changes
Dynamic or change is fallen within scope of the presently claimed invention.
Embodiment 1
（1）, the mushroom inclined-plane parent species of choosing artificial culture carry out shake-flask seed culture；
Culture medium prescription used is：15 grams of peptone, 1 gram of yeast extract, 10 grams of maltose, 10 grams of glucose, 50 grams of wheat bran, phosphorus
1.5 grams of acid dihydride potassium, 0.75 gram of magnesium sulfate, 0.01 gram of vitamin B1,1000 milliliters of water, pH value 6.5；
Each raw material is accurately weighed by formula ratio, adds 1300 milliliters of water to dissolve by heating, is settled to 1000 milliliters, wheat bran adds water logging
Boil, 30 ~ 40 minutes under warm fire, filter to take filtered juice, fully mix rear high pressure steam sterilization thoroughly, after cooling aseptically, by oblique
The ratio between face and shaking flask are 1：6 ratio inoculation；26~25 DEG C of cultivation temperature, the rotating speed of rotary shaker is 170 ~ 200 revs/min
Clock；130 ~ 160 revs/min of rotating speed early stage of Clothoid type shaking table, 160 revs/min of later stage, culture terminate 9 ~ 10 days time；
（2）, seed tank culture：
Used medium formula is：Add 2000 grams of potato starch, 2000 grams of wheat bran, 4000 grams of glucose, soya-bean cake in 1000 liters of tanks
1000 grams of powder, 8000 grams of brown sugar, 500 grams of yeast extract, 800 grams of potassium dihydrogen phosphate, 800 grams of magnesium sulfate, vitamin B1 800, disappear
150 milliliters -200 milliliters of infusion, substantive 700 liters of amount of solution；
Ferment pH value 5.0---6.0；Steam pressure 0.13Mpa ~ 0.15Mpa in incubator, 123 DEG C ~ 126 DEG C of culture medium temperature,
Retention time 45min, sterilizing terminate 24 DEG C~28 DEG C of cooling；Flame is protected inocalation method to put into strain in tank and is inoculated with；Liquid bacteria
Kind condition of culture：Ventilation is adjusted, is adjusted ventilation to 1 by intake valve and air bleeding valve：0.5V/V~1.2V/V（Feed liquid body
Product/volume of air）；Tank pressure is adjusted, regulation tank pressure is maintained between 0.02Mpa~0.04Mpa；Cultivation temperature regulation 24 DEG C~
In the range of 25 DEG C；Culture 4d~5d, which can reach, under these conditions puts tank index, standby；
（3）, ingredient requirement：Soya bean straw is crushed with rubbing type pulverizer；It is formulated and is：77 parts of soya bean straw, 10 parts of wheat bran, maize flour
3 parts, 6 parts of beancake powder, 1 part of gypsum, 1 part of white sugar, 0.8 part of salt, 1.2 parts of calcium superphosphate；
（4）, spice pack：Culture medium is got out every raw and auxiliary material by formula rate, will be got ready by the specification of production requirement
Raw material after screening is directly transferred to be sufficiently stirred with mixing in transit mixer by proportioning by conveyer belt；The raw material after 20 minutes
Stirring finishes, and Compost moisture content control is in 58-60%, pH value 7.5-8.0, pack；
（5）, sterilizing：Autoclave cabinet sterilizes, and sterilizing cabinet must drain cold air before insulated sterilizing, make sterilization kettle temperature uniform
Unanimously, dead angle is not stayed, 2 h are kept between 0.12 Mpa；
（6）, cooling：To room temperature；
（7）, inoculation：Temperature is kept for 18 ~ 20 DEG C；The each vaccination 20ml~30ml of inoculum concentration；
（8）, educate bacterium culture：15 d interior room temperature control systems are at 26 DEG C ~ 28 DEG C after inoculation, 15 ~ 30 d temperature controls at 22 DEG C ± 2 DEG C,
20 DEG C ± 2 DEG C of temperature after 30 d；Relative air humidity control is advisable 45~60%）；
（9）, be granulated：Ke Li Lu feeds.
Embodiment 2
1st, the mushroom inclined-plane parent species for choosing artificial culture carry out shake-flask seed culture；
2nd, seed tank culture：Inocalation method is protected using flame, pyrosphere is placed on above dog-house and lighted, now opens charging aperture
Lid, rapidly puts into strain in tank, then, feeding lid is covered rapidly, tightened.Flame ring is removed, inoculation finishes.Liquid bacteria
Kind condition of culture：Ventilation is adjusted, is adjusted ventilation to 1 by intake valve and air bleeding valve：0.5V/V~1.2V/V (feed liquid bodies
Product/volume of air).Tank pressure is adjusted, regulation tank pressure is maintained between 0.02Mpa~0.04Mpa.Cultivation temperature regulation 24 DEG C~
In the range of 25 DEG C.Culture 4d~5d, which can reach, under these conditions puts tank index, standby；
3rd, ingredient requirement：To meet NY 5009 regulation using cultivation matrix and various auxiliary materials.Select fresh nothing go mouldy soya bean straw use
(long generous mm) 2 × 2 × 3-4 × 4 × 3.2 after rubbing type pulverizer crushes；It is formulated and is：Soya bean straw 77kg, the kg of wheat bran 10,
The kg of maize flour 3, the kg of beancake powder 6, the kg of gypsum 1, the kg of white sugar 1, the kg of salt 0.8, the kg of calcium superphosphate 1.2；
4th, spice packs：Culture medium is got out every raw and auxiliary material by formula rate, sieve will be got ready by the specification of production requirement
Raw material after choosing is directly transferred to be sufficiently stirred with mixing in transit mixer by proportioning by conveyer belt.Raw material stirs after 20 minutes
Mix and finish, Compost moisture content control is in 58-60%, and 7.5~8.0. of pH value is from the cm specifications of the cm of 16.2 cm × 36 × 0.035
Low pressure ethylene knuckle bag；Being packed using automatic socket mouth plunger sack filling machine, bacterium bag is filled to 20 centimeters of height, weight in wet base 1.1~
1.15 kilograms, install put after bag it is subject to sterilization in basket；
5th, sterilize：By charged culture bag, it is sent in sterilization bin with sterilizing tote cart, is sterilized using Large-scale High-Pressure sterilizing cabinet,
Sterilizing cabinet must drain cold air before insulated sterilizing, after making elimination bacterium, treat high-pressure sterilizing pot, when pressure gauge is down to 0, open
Pot door, radiates to 20 minutes and starts to take the dish out of the pot, and enters cooling chamber cooling；
6th, cool down：Cooling chamber must carry out cleaning-sterilizing, install air cleaner, and 300,000 grades of degree of purifications of precooling room are strong cold
At least keep 10000 grades of degree of purification；Refrigeration machine in cooling chamber should be arranged to interior circulation, it is desirable to which power is big, and cooling is fast, most
Cultivating bag is down to suitable temperature in the short time.The exchange rate of air can be reduced, reduces the risk of pollution；
7th, it is inoculated with：Using laminar flow supply air system and return air filtration system, the air velocity for keeping transfer room certain reclaims miscellaneous bacteria
Onto filter, ensure the cleanliness of interior.Blown from strong cold house, reduce influence of the humidity to laminar flow hood high efficiency particulate air filter.Using
Special airflow design, certain barometric gradient is kept, reduce the infection rate of bacterium bag.Transfer room indoor temperature keeps 18~20
℃；Due to there is cultigen to transmit to outer operating area during inoculation, certain barotropic state must be kept so indoor, and fresh air
Introducing has to pass through high efficiency filter, indoor 10000 grades of holding, and inoculation device region is kept for 100 grades；People during inoculation operation
Member must be carried out by sterile working requirement, liquid spawn inoculator be opened, into automatic disinfection program；Bacterium bag to be seeded is put
Inoculator conveyer belt is put, is inputted in the inoculation cabin of inoculator；In inoculator inoculation cabin, liquid spawn is injected into bacterium with inoculating gun
In bag；Perform inoculator inoculation code；It is swift in motion, accurately；The each vaccination 20ml~30ml of inoculum concentration；
8th, bacterium culture is educated：Bacteria room, which has, to be incubated with air exchange function, and the bacterium bag after inoculation is straight by frame restocking with fork truck in time
It is vertical to put.Educating bacterium room control device mainly includes the control system of temperature control system, ventilation (CO2) control system and air humidity
System.15 d interior room temperature control systems are at 26 DEG C ~ 28 DEG C after inoculation, and 15 ~ 30 d temperature controls are at 22 DEG C ± 2 DEG C, 20 DEG C of temperature after 30 d
±2℃；Relative air humidity control is advisable 45~60%）；Bacterium germination room needs lucifuge.Appropriate ventilation.Using lower return side
The air-cooler of air-supply, air-supply is soft, reduces the temperature difference in storehouse.PLC controllers can control temperature, humidity, CO simultaneously2 Concentration, pass through
The regulation of each parameter controls the growth of mycelia at any time.Special airflow design and control method can effectively avoid temperature, humidity,
CO2Upper lower leaf, ensure the uniformity of mycelial growth.To be cultivated through 30 days, bacterium bag visual examination mycelia is pure white, in short flannel hairy,
Uniformly flexible up and down, tubbiness, close neat, bag is without ecru ponding, and do not dry up shrinkage phenomenon, and sack is down at charge level sky bag
There are many glistening droplets, no living contaminants, microscopy mycelia color depth, and branch is more, and crotch angle is big, there is clamp connection,
Growing point is clearly visible.Cultivating in a fungus bag terminates.
9th, it is granulated：It is granulated using the special prilling process of the feed of new high-efficiency, the good bacterium bag of fermented and cultured is removed
Be put into pelletizing forming equipment after polybag, grain products without obvious mechanical admixture, it is uniform in size, have a fragrant, pelletizing forming, then
Lu feeds are prepared and finished.
Test example 1
It is bred as public sika deer feeding experiment
Feeding effect：Test and carried out on December 1st, 2015 on 2 1st, 2016, entered in the big sub- deer field of battalion in Changchun Shuangyang District
OK, 20 incubation sika deers are fed, feed two processing of Lenlinus edodes Ke Li Lu feeds and Chang Gui Lu feed of the present invention, feeding experiment
It is set as 60 days, it is 8 days preliminary trial periods, positive to try 52 days phases, while the measurement of body weight, body chi is carried out to being bred as sika deer.
Feeding materials and animal：Experimental animal and deer field conventional feed are provided by the big sub- deer field of battalion in Changchun Shuangyang District, fragrant
Mushroom grain Lu feeds are provided by scientific research institution of Yan Biandekang Bioisystech Co., Ltd, and it is to produce for 2013 that experiment, which is bred as sika deer,
The incubation of 15~16 monthly age 20 public sika deers, Suo You Lu select health, body weight similar（P>0.05）Experimental animal point
Group, it is respectively 10.Conventional feed is maize flour 67%, soya-bean cake 30%, salt 1.2%, bone meal 1%, B B-complex 0.8%.
Experiment is bred as sika deer management：Experiment be bred as sika deer be rear livestock in pens, Lenlinus edodes Ke Li Lu feeds of the present invention with
The daily scale of feeding of conventional feed, respectively 1050g/ heads .d, 1180g/ head .d, by poultry raiser 8：00、15：00、21：30 point three
Secondary to feed, roughage is robur leaf, blue or green storage corn stalk, 24 hours free choice feedings, average scale of feeding 4.78kg/ heads .d, is freely drunk
Water.
Body weight, body measurement and output of pilose antler：At the beginning and end of feeding experiment, sika deer platform balance is bred as to each group
Empty stomach check weighing is carried out, with meter ruler measurement body is high, chest depth, weighed with scale when sawing pilose antler and records Mei Tou Lu fresh output of pilose antler.
It is bred as Mei Hua Lu Lu body weight gains：The average day total augment weight of Lenlinus edodes Ke Li Lu feeds of the present invention, conventional feed is respectively
68.43 g、63.28 g。
It is bred as sika deer Body size measurements result：Lenlinus edodes Ke Li Lu feeds of the present invention, conventional feed feeding , Lu body length increase
Respectively 6.58cm, 6.21cm；Body high growth is respectively 3.21 cm, 3.2 cm；It is respectively 5.21 cm, 4.94 that chest depth, which increases,
cm。
Output of pilose antler result：Feeding conventional feed saws the fine and soft time as 15 days~August in July 6 days, feeding Lenlinus edodes of the present invention
The Li Lu feed times are 2 days~August of August 12；The feeding Lenlinus edodes Ke Li Lu feeds saw pilose antler time of the present invention substantially shortens.Raise
With Lenlinus edodes Ke Li Lu feeds of the present invention, total yield pilose antler amount is 510.4g/ heads, and conventional feed total yield pilose antler amount is 480.9g/ heads；
For Lenlinus edodes Ke Li Lu feeds of the present invention in terms of incubation sika deer production performance is improved, effect is better than conventional feed, has adapted to work as
Preceding stag breeding is advantageous to the utilization of feed resource to intensive, scale, the demand of high-quality and efficient operation and development.
Test example 2
Influence to being bred as sika deer feed intake and protein metabolism rate
The experimental animal selection 6 monthly age male sika deer son Lu 4 that body condition is good, body weight is similar.Tested on June 15th, 2016
~August is carried out in plant of Shuan Yangqu Lu townshiies of Jilin Province on the 5th.
Daily ration is tested using Lenlinus edodes Ke Li Lu feeds of the present invention as raw material.
Test method and feeding management：4X4 Latin―Square designs are taken in this experiment, respectively by 4 sika deer stable breedings in
In the cage of 1.8X2.5 rice, experiment is carried out 4 periods altogether to early August from mid-June, and each issue is 13 days, including 10 days preliminary trial periods
With positive 3 days phases of examination, Lenlinus edodes Ke Li Lu feeds of the present invention are fed successively.
Sample collection and DATA REASONING：This experiment collects fecaluria using full fecaluria method of receiving, in the just examination phase daily before feeding
Collect and differentiate, urine is each just being tried the phase and collected 1 time（10% concentrated sulfuric acid, 20 milliliters of fixed nitrogen are added in bucket before collecting urine sample）.Simultaneously
Measure the feed intake of every sika deer.After excrement sample is weighed plus after 10% dense sulfuric acid treatment, in 60~70 DEG C of drying, 40 are crossed after crushing
Mesh sieve is standby.Accurate measurement and the total volume of urine for recording each positive examination phase, and total volume of urine 10% is fitted into -20 DEG C of preservations in plastic bottle
It is to be measured.
Sample surveys fixed sum data analysis：The assay method of reference laboratories conventional nutrient index, determine and calculate excrement sample,
Dry, crude protein in urine sample, the data obtained is arranged using Excel (2003) and initial analysis, calculates each experiment week
Indices in phase.
Interpretation of result
Influence to sika deer apparent digestibility of the dry matter：DM feed intakes（Gram/day）For 1638 ± 16.20b;DM discharge rates（Gram/
My god）For 530 ± 6.88b；DM digestibilities（%）For 69.49 ± 1.19a.
Influence to sika deer protein metabolism rate：Feed Lenlinus edodes Ke Li Lu feed protein feed intakes of the present invention（Gram/
My god）For 264.83 ± 3.78ab, fecal nitrogen discharge rate（Gram/day）For 13.50 ± 0.54b, urinary nitrogen discharge rate（Gram/day）For 9.78 ±
0.56c;Protein deposit volume（Gram/day）For 113.40 ± 1.22a;Nitrogen metabolic rate is 44.12 ± 0.86b.
This experiment passes through 4X4 Latin square metabolic tests, have studied Lenlinus edodes Ke Li Lu feeds of the present invention to sika deer albumen
The influence of matter digestion and metabolism.Result of the test shows：Feeding Lenlinus edodes grain Lu feeds can significantly improve the metabolic rate of protein.
Protein feeds is saved, feeding cost is reduced and reduces pollution of the sika deer excreta to environment.
Claims (3)
1. one kind makees matrix using Lenlinus edodes with soya bean straw prepares Ke Li Lu feeds, it is characterised in that：
The granulation of Lenlinus edodes strain fermentation is accessed thereto using soya bean straw as material to form, strain access amount is 20%, Lenlinus edodes
Every 100 grams of Ke Li Lu feeds contain 91.3 grams of dry, 17.66 grams of crude protein, 0.99 gram of crude fat, 1.12 grams of calcium, 0.53 gram of phosphorus,
15.24 grams of energy.
2. it is as claimed in claim 1 it is a kind of the preparation technology that matrix prepares Ke Li Lu feeds is made with soya bean straw using Lenlinus edodes,
It is characterized in that comprise the following steps：
1）Choose mushroom inclined-plane parent species and carry out shake-flask seed culture；
2）Seed tank culture：Inocalation method is protected using flame, by step 1）Shake-flask seed carry out seed tank culture it is standby；
3）77 parts of soya bean straw, 10 parts of wheat bran, 3 parts of maize flour, 6 parts of beancake powder, 1 part of gypsum, 1 part of white sugar, salt are weighed in proportion
0.8 part, 1.2 parts of spices that stir of calcium superphosphate, sterilizing, cooling, inoculation be made bacterium bag, each vaccination 20ml of inoculum concentration~
30ml；
4）Educate bacterium culture：
15 days interior room temperature control systems are at 26 DEG C ~ 28 DEG C after inoculation；15 ~ 30 days temperature controls are at 22 DEG C ± 2 DEG C；20 DEG C of temperature after 30 days
±2℃；Relative air humidity is controlled 45~60%；Bacterium germination room lucifuge；Cultivating in a fungus bag terminates；
5）It is granulated：Take out step 4）Bacterium material in bacterium bag, the particle manufacture craft system through routine is into Lu feeds.
3. it is as claimed in claim 1 it is a kind of the preparation technology that matrix prepares Ke Li Lu feeds is made with soya bean straw using Lenlinus edodes,
It is characterized in that comprise the following steps：
1）, the mushroom inclined-plane parent species of choosing artificial culture carry out shake-flask seed culture；
Culture medium prescription used is：15 grams of peptone, 1 gram of yeast extract, 10 grams of maltose, 10 grams of glucose, 50 grams of wheat bran, phosphorus
1.5 grams of acid dihydride potassium, 0.75 gram of magnesium sulfate, 0.01 gram of vitamin B1,1000 milliliters of water, pH value 6.5；
Each raw material is accurately weighed by formula ratio, adds 1300 milliliters of water to dissolve by heating, is settled to 1000 milliliters, wheat bran adds water logging
Boil, 30~40 minutes under warm fire, filter to take filtered juice, fully mix rear high pressure steam sterilization thoroughly, after cooling aseptically, by oblique
The ratio between face and shaking flask are 1：6 ratio inoculation；26~25 DEG C of cultivation temperature, the rotating speed of rotary shaker is 170~200 revs/min
Clock；130~160 revs/min of rotating speed early stage of Clothoid type shaking table, 160 revs/min of later stage, culture terminate 9~10 days time；
2）, seed tank culture：
Used medium formula is：Add 2000 grams of potato starch, 2000 grams of wheat bran, 4000 grams of glucose, soya-bean cake in 1000 liters of tanks
1000 grams of powder, 8000 grams of brown sugar, 500 grams of yeast extract, 800 grams of potassium dihydrogen phosphate, 800 grams of magnesium sulfate, vitamin B1 800, disappear
150 milliliters -200 milliliters of infusion；
Ferment pH value 5.0---6.0；Steam pressure 0.13Mpa ~ 0.15Mpa in incubator, 123 DEG C ~ 126 DEG C of culture medium temperature,
Retention time 45min, sterilizing terminate 24 DEG C~28 DEG C of cooling；Flame is protected inocalation method to put into strain in tank and is inoculated with；Liquid bacteria
Kind condition of culture：Ventilation is adjusted, is adjusted ventilation to 1 by intake valve and air bleeding valve：0.5V/V~1.2V/V（Feed liquid body
Product/volume of air）；Tank pressure is adjusted, regulation tank pressure is maintained between 0.02Mpa~0.04Mpa；Cultivation temperature regulation 24 DEG C~
In the range of 25 DEG C；Culture 4d~5d reaches that to put tank index standby under these conditions；
3）, ingredient requirement：Soya bean straw is crushed with rubbing type pulverizer；It is formulated and is：77 parts of soya bean straw, 10 parts of wheat bran, maize flour 3
Part, 6 parts of beancake powder, 1 part of gypsum, 1 part of white sugar, 0.8 part of salt, 1.2 parts of calcium superphosphate；
4）, spice pack：Culture medium is got out every raw and auxiliary material by formula rate, sieve will be got ready by the specification of production requirement
Raw material after choosing is directly transferred to be sufficiently stirred with mixing in transit mixer by proportioning by conveyer belt；Raw material stirs after 20 minutes
Mix and finish, Compost moisture content control is in 58-60%, pH value 7.5-8.0, pack；
5）, sterilizing：Autoclave cabinet sterilizes, and sterilizing cabinet must drain cold air before insulated sterilizing, make sterilization kettle temperature uniform
Unanimously, dead angle is not stayed, 2 h are kept between 0.12 Mpa；
6）, cooling：To room temperature；
7）, inoculation：Temperature is kept for 18 ~ 20 DEG C；The each vaccination 20ml~30ml of inoculum concentration；
8）, educate bacterium culture：15 d interior room temperature control systems are at 26 DEG C ~ 28 DEG C after inoculation, 15 ~ 30 d temperature controls at 22 DEG C ± 2 DEG C,
20 DEG C ± 2 DEG C of temperature after 30 d；Relative air humidity 45~60%；
9）, be granulated：Ke Li Lu feeds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710727297.5A CN107333982A (en) | 2017-08-23 | 2017-08-23 | A kind of method for making matrix Bei Lu feeds with soya bean straw using Lenlinus edodes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710727297.5A CN107333982A (en) | 2017-08-23 | 2017-08-23 | A kind of method for making matrix Bei Lu feeds with soya bean straw using Lenlinus edodes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107333982A true CN107333982A (en) | 2017-11-10 |
Family
ID=60214029
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710727297.5A Pending CN107333982A (en) | 2017-08-23 | 2017-08-23 | A kind of method for making matrix Bei Lu feeds with soya bean straw using Lenlinus edodes |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107333982A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113317393A (en) * | 2021-06-03 | 2021-08-31 | 焦作市佰役安生物工程有限公司 | Method for preparing fermentation type feed additive by using gas explosion |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102389023A (en) * | 2011-09-04 | 2012-03-28 | 蒲增军 | Straw feed protein bacterium and manufacture method thereof |
| CN103168921A (en) * | 2012-06-18 | 2013-06-26 | 南开大学 | Method for producing straw feed |
| CN103652353A (en) * | 2013-11-24 | 2014-03-26 | 何寒 | Method for preparing feed by taking edible fungi mycelia as raw materials |
| CN104719665A (en) * | 2015-02-16 | 2015-06-24 | 江西农业大学 | Edible fungus processing feed capable of improving utilization rate of ruminants for rape stalks and preparation method of edible fungus processing feed |
| CN105385607A (en) * | 2015-12-18 | 2016-03-09 | 湖北五林中地农业科技有限公司 | Lentinus edodes liquid submerged fermentation culture medium formula and fermentation technology |
| CN106386163A (en) * | 2016-08-15 | 2017-02-15 | 韦中定 | Method for cultivating edible fungi by using crop straw |
| CN106912685A (en) * | 2017-01-20 | 2017-07-04 | 中国科学院东北地理与农业生态研究所 | A kind of preparation method of the full mixed feed of myceliaization stalk high-quality |
| CN106942477A (en) * | 2017-03-31 | 2017-07-14 | 沙洋县汉江牛业发展有限公司 | A kind of cattle diet using mushroom mushroom bran as raw material and its preparation method and application |
-
2017
- 2017-08-23 CN CN201710727297.5A patent/CN107333982A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102389023A (en) * | 2011-09-04 | 2012-03-28 | 蒲增军 | Straw feed protein bacterium and manufacture method thereof |
| CN103168921A (en) * | 2012-06-18 | 2013-06-26 | 南开大学 | Method for producing straw feed |
| CN103652353A (en) * | 2013-11-24 | 2014-03-26 | 何寒 | Method for preparing feed by taking edible fungi mycelia as raw materials |
| CN104719665A (en) * | 2015-02-16 | 2015-06-24 | 江西农业大学 | Edible fungus processing feed capable of improving utilization rate of ruminants for rape stalks and preparation method of edible fungus processing feed |
| CN105385607A (en) * | 2015-12-18 | 2016-03-09 | 湖北五林中地农业科技有限公司 | Lentinus edodes liquid submerged fermentation culture medium formula and fermentation technology |
| CN106386163A (en) * | 2016-08-15 | 2017-02-15 | 韦中定 | Method for cultivating edible fungi by using crop straw |
| CN106912685A (en) * | 2017-01-20 | 2017-07-04 | 中国科学院东北地理与农业生态研究所 | A kind of preparation method of the full mixed feed of myceliaization stalk high-quality |
| CN106942477A (en) * | 2017-03-31 | 2017-07-14 | 沙洋县汉江牛业发展有限公司 | A kind of cattle diet using mushroom mushroom bran as raw material and its preparation method and application |
Non-Patent Citations (2)
| Title |
|---|
| 李野等: "4株食用菌发酵小麦秸秆粗蛋白含量研究", 《饲料研究》 * |
| 热沙来提汗？买买提等: "食用担子菌接种对棉花秸秆消化性改善效果研究", 《新疆农业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113317393A (en) * | 2021-06-03 | 2021-08-31 | 焦作市佰役安生物工程有限公司 | Method for preparing fermentation type feed additive by using gas explosion |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105054261B (en) | A kind of waste matter of fermenting produces the apparatus and method for of high activity high nutrition feed | |
| CN101066090B (en) | Aweto activated reinforced immunological feed additive and its production process | |
| CN102090345B (en) | Ultrathin fermentation bed and used fermentation bed microbial inoculum | |
| CN103798503B (en) | The ruminant Tiny ecosystem functional feed of composite zymocyte liquid and preparation method | |
| CN103404370B (en) | The method of edible fungus species is cultivated with pelelith | |
| CN103804098B (en) | A kind of straw mushroom medium and cultivating straw mushroom method thereof | |
| CN104877937A (en) | Bacillus amyloliquefaciens bacterial fertilizer for promoting growth of capsicum annuum and preparation method and applications thereof | |
| CN101669583A (en) | Method for preparing novel complex enzyme feed additive | |
| CN104017739B (en) | Complex micro organism fungicide, its preparation method and its application in high protein potato dreg fodder is produced | |
| CN101912040A (en) | Tomato pomace biological fermentation feed and preparation method thereof | |
| CN102224792B (en) | Aquaculture method of selenium-rich hericium erinaceus | |
| CN100408673C (en) | Organic microbial composite and use | |
| CN102948324A (en) | Method for producing edible mushroom by used biological packing | |
| CN104761386B (en) | The compost and preparation method of a kind of utilization giant knotweed rhizome residue Xinbao mushroom culturing | |
| CN107365727A (en) | Organic fertilizer fermentation agent | |
| CN103976147B (en) | Lactobacillus acidophilus pomace fermented feed and production technology thereof | |
| CN101715875B (en) | Protein feed prepared by adopting rapeseed dregs and preparation method thereof | |
| CN106306359A (en) | Method for preparing feed for grass feed animal through straw fermentation | |
| CN104774787A (en) | Compounding method of microorganism agent for chicken manure fermentation | |
| CN107853071A (en) | A kind of method using Chinese pennisetum factory culture pleurotus eryngii | |
| CN101962622A (en) | Complex bacteria inoculant for preparing organic fertilizer from effluents of animal slaughter houses and breeding plants serving as raw materials and preparation method thereof | |
| CN106747839A (en) | A kind of culture medium of edible fungus, its preparation method and application | |
| CN102174460A (en) | Gliocladium roseum chlamydospore and method for producing wettable powder thereof | |
| CN104381615B (en) | Produce the method and product of aweto feed addictive | |
| CN109619265A (en) | A kind of method of selenium-enriched cordceps militaris edible fungi residue feed preparation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171110 |
|
| RJ01 | Rejection of invention patent application after publication |