CN107250348B - 使用表达lgr4、lgr5和lgr6的上皮干细胞在组织应用中开发和使用最小极性化功能细胞微聚集体单元的方法 - Google Patents

使用表达lgr4、lgr5和lgr6的上皮干细胞在组织应用中开发和使用最小极性化功能细胞微聚集体单元的方法 Download PDF

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CN107250348B
CN107250348B CN201580075326.3A CN201580075326A CN107250348B CN 107250348 B CN107250348 B CN 107250348B CN 201580075326 A CN201580075326 A CN 201580075326A CN 107250348 B CN107250348 B CN 107250348B
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lgr
lgr6
wound
cells expressing
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CN107250348A (zh
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D.M.洛
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RegenETP Inc
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N1/12—Chemical aspects of preservation
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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    • A01N1/12—Chemical aspects of preservation
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/362—Skin, e.g. dermal papillae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3813—Epithelial cells, e.g. keratinocytes, urothelial cells
    • A—HUMAN NECESSITIES
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    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
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    • A61L2430/00—Materials or treatment for tissue regeneration
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CN201580075326.3A 2014-12-02 2015-12-01 使用表达lgr4、lgr5和lgr6的上皮干细胞在组织应用中开发和使用最小极性化功能细胞微聚集体单元的方法 Active CN107250348B (zh)

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Application Number Priority Date Filing Date Title
US201462086526P 2014-12-02 2014-12-02
US62/086526 2014-12-02
US14/954,335 US10926001B2 (en) 2014-12-02 2015-11-30 Methods related to minimally polarized functional units
US14/954335 2015-11-30
PCT/US2015/063114 WO2016089825A1 (en) 2014-12-02 2015-12-01 Methods for development and use of minimally polarized function cell micro-aggregate units in tissue applications using lgr4, lgr5 and lgr6 expressing epithelial stem cells

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CN107250348A CN107250348A (zh) 2017-10-13
CN107250348B true CN107250348B (zh) 2021-09-03

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Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201908271WA (en) * 2017-03-14 2019-10-30 Juno Therapeutics Inc Methods for cryogenic storage
AU2019212976A1 (en) * 2018-01-26 2020-07-23 Polarityte, Inc. Complex living interface-coordinated self-assembling materials (CLICSAM)
EP3743125A4 (en) * 2018-01-26 2022-03-02 Polarityte, Inc. BIOMATERIAL ACCELERATING SUBSTRATE WITH COMPOSITE INTERFACE
FR3082123B1 (fr) 2018-06-07 2020-10-16 Urgo Rech Innovation Et Developpement Pansement cellularise et son procede de fabrication
JP2024006681A (ja) * 2022-07-04 2024-01-17 学校法人近畿大学 生体材料
WO2025166131A1 (en) * 2024-02-01 2025-08-07 Board Of Regents, The University Of Texas System Compositions and methods for treating bone and muscle injuries

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2412800A1 (en) * 2010-07-29 2012-02-01 Koninklijke Nederlandse Akademie van Wetenschappen Liver organoid, uses thereof and culture method for obtaining them
CN103550828A (zh) * 2013-10-17 2014-02-05 中国科学院动物研究所 一种基于毛囊干细胞和硅凝胶敷料的皮肤重建方法

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040126881A1 (en) 2002-09-06 2004-07-01 Vincent Ronfard Fibrin cell supports and methods of use thereof
WO2006008009A2 (en) 2004-07-23 2006-01-26 Bayer Healthcare Ag Diagnostics and therapeutics for diseases associated with leucine-rich repeat-containing gpcr 6 (lgr6)
US20060134074A1 (en) 2004-08-30 2006-06-22 Naughton Gail K Compositions and methods for promoting hair growth
EP1942957B1 (en) * 2005-10-27 2012-07-11 Coloplast A/S Biodegradable scaffold with ecm material
WO2008008009A1 (en) 2006-07-13 2008-01-17 St. Jude Medical Ab Medical information management in a patient information hub system
US8366723B2 (en) 2006-08-03 2013-02-05 Rassman Licensing, Llc Hair harvesting device and method with localized subsurface dermal fluid insertion
US7998737B2 (en) 2006-09-12 2011-08-16 Deutsches Krebsforschungszentrum Cell culture of keratinocytes under non-differentiating conditions
US9752124B2 (en) 2009-02-03 2017-09-05 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium for epithelial stem cells and organoids comprising the stem cells
GB201111244D0 (en) 2011-06-30 2011-08-17 Konink Nl Akademie Van Wetenschappen Knaw Culture media for stem cells
FI20095355A0 (fi) 2009-04-01 2009-04-01 Helsingin Yliopisto Regeneroituva aktiivinen matriisi ja sen käytöt
CN102498221A (zh) 2009-06-25 2012-06-13 株式会社资生堂 基于aff-4表达筛选抗白发剂的方法
US20110130711A1 (en) 2009-11-19 2011-06-02 Follica, Inc. Hair growth treatment
JP6023049B2 (ja) 2010-06-30 2016-11-09 チェン，ジンシー 皮膚組織細胞の集合体の製造方法及びその用途
GB201106395D0 (en) 2011-04-14 2011-06-01 Hubrecht Inst Compounds
EP2771454B1 (en) 2011-10-27 2019-07-24 Universität Leipzig Method for deriving melanocytes from the hair follicle outer root sheath and preparation for grafting
JP6186997B2 (ja) 2012-08-07 2017-08-30 日油株式会社 塗布型帯電防止剤
US9655930B2 (en) 2012-10-01 2017-05-23 Aderans Research Institute, Inc. Compositions and methods for producing reconstituted skin
US20140106447A1 (en) 2012-10-12 2014-04-17 Mimedx Group, Inc. Compositions and methods for recruiting stem cells
LT2970890T (lt) 2013-03-14 2020-07-10 The Brigham And Women`S Hospital, Inc. Kompozicijos ir būdai, skirti epitelinių kamieninių ląstelių padauginimui ir kultivavimui
US9592257B2 (en) 2013-10-11 2017-03-14 MWV Cell, LLC Complete human skin organ generated from culture-expanded cells
TWI548413B (zh) 2014-11-07 2016-09-11 國立臺灣大學 誘導毛囊新生的皮膚萃取物、組合物及其用途

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2412800A1 (en) * 2010-07-29 2012-02-01 Koninklijke Nederlandse Akademie van Wetenschappen Liver organoid, uses thereof and culture method for obtaining them
CN103550828A (zh) * 2013-10-17 2014-02-05 中国科学院动物研究所 一种基于毛囊干细胞和硅凝胶敷料的皮肤重建方法

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GB2569056B (en) 2020-01-01
CA2969707A1 (en) 2016-06-09
US20190046691A1 (en) 2019-02-14
JP2019088299A (ja) 2019-06-13
CA2969707C (en) 2019-08-20
EP3227431C0 (en) 2025-01-29
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IL287488B2 (en) 2023-06-01
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AU2019240603A1 (en) 2019-10-17
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ZA201703907B (en) 2023-03-29
BR112017011808A2 (pt) 2018-02-27
JP6791854B2 (ja) 2020-11-25
MX2017007243A (es) 2018-01-25
CN107250348A (zh) 2017-10-13
SG11201704502RA (en) 2017-06-29
US20180154044A1 (en) 2018-06-07
EP4438128A2 (en) 2024-10-02
US11596714B2 (en) 2023-03-07
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KR20210043024A (ko) 2021-04-20
AU2023202857A1 (en) 2023-05-25
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US11338060B2 (en) 2022-05-24
HK1250515A1 (zh) 2018-12-21
US20160151540A1 (en) 2016-06-02
GB2569056A (en) 2019-06-05
EP3227431B1 (en) 2025-01-29
AU2015355187B2 (en) 2019-10-03
JP2017538411A (ja) 2017-12-28
SG10201914058QA (en) 2020-03-30
KR20180136587A (ko) 2018-12-24
KR20170098844A (ko) 2017-08-30
US10926001B2 (en) 2021-02-23
CR20170296A (es) 2018-01-25
EA201791125A1 (ru) 2018-06-29
US11266765B2 (en) 2022-03-08
AU2019240603B2 (en) 2023-02-09
US20210322643A1 (en) 2021-10-21
US20230181797A1 (en) 2023-06-15
GB201710646D0 (en) 2017-08-16
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US20180154043A1 (en) 2018-06-07
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AU2015355187C1 (en) 2020-02-20
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