CN107208088A - 酵母中的油酸生产 - Google Patents

酵母中的油酸生产 Download PDF

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CN107208088A
CN107208088A CN201580073772.0A CN201580073772A CN107208088A CN 107208088 A CN107208088 A CN 107208088A CN 201580073772 A CN201580073772 A CN 201580073772A CN 107208088 A CN107208088 A CN 107208088A
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V·塔萨科拉科莱兹
E·E·不雷夫诺瓦
J·弗里德兰德
A·卡明埃尼
A·J·肖四世
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Abstract

本发明公开了转化的细胞，所述细胞包含影响所述细胞的脂质含量的一种或多种遗传修饰，这例如通过相对于同一类型的未修饰的细胞增加所述细胞中油酸的浓度来实现。还公开了用于改变细胞的脂质含量的方法，所述方法是通过提高所述细胞中一种或多种蛋白质的活性和/或通过降低所述相同细胞中一种或多种蛋白质的活性来实现的。

Description

酵母中的油酸生产
相关申请
本申请要求2014年12月10日提交的美国临时专利申请序列号62/090,169的优先权益。
序列表
本申请含有序列表，该序列表已经以ASCII格式以电子方式递交并且在此以引用的方式整体并入本文。所述在2015年12月8日创建的ASCII拷贝被命名为NGX-03725_SL.txt并且具有523,236个字节的大小。
背景技术
脂质是食品和化妆品行业中不可缺少的成分，并且它们是生物柴油和生物化学行业中重要的前体。包括被良好表征的酵母解脂耶氏酵母(Yarrowia lipolytica)在内的许多含油微生物产生脂质。
微生物合成具有不同的碳链长度和不饱和度的脂质。这些脂肪酸可以作为贮存脂质，例如作为三酰基甘油酯(TAG)被贮存在细胞器中，被称作脂质体或脂滴。细胞的脂质谱，即构成细胞中总脂质的脂肪酸物质的相对量，是通过合成脂肪酸的各种酶(脂肪酸合酶、延长酶、去饱和酶)、通过将脂肪酸并入到贮存脂质中来使它们稳定的各种酶(酰基转移酶)以及降解脂肪酸并且贮存脂质的各种酶(例如脂酶)的活性和底物特异性来确定的。
在使脂质产品以特定的市场/应用为目标时，能够调控细胞的脂质谱以提高特定的脂肪酸的浓度的能力是所期望的。确切地说，提高含油酵母，如解脂耶氏酵母的油酸含量提高了生物体中产生的TAG的值。
含油生物体的脂质收率可以通过牵涉到脂质途径中的基因的上调、下调、或缺失来增加。然而，成功调节酶最好也只不过是不可预测的。举例来说，在解脂耶氏酵母中使来自高山被孢霉(Mortierella alpine)的DGA1过表达对脂质含量没有显著的影响(美国专利7,198,937；以引用的方式并入本文)；同样，使DGA2过表达在不存在其它遗传修饰的情况下对脂质含量没有显著的影响。
发明内容
在一些方面，本发明涉及一种转化的细胞，其中所述细胞选自由以下各项组成的组：藻类、细菌、霉菌、真菌、植物、以及酵母。所述细胞可以是酵母。举例来说，所述细胞可以是选自由以下各项组成的组的酵母：噬腺嘌呤阿克苏拉酵母(Arxula adeninivorans)、酿酒酵母(Saccharomyces cerevisiae)、以及解脂耶氏酵母。
在一些实施方案中，所述转化的细胞包含提高所述细胞中一种或多种蛋白质的活性的一种或多种遗传修饰。举例来说，所述转化的细胞可以包含提高以下各项的活性的一种或多种遗传修饰：Δ9去饱和酶蛋白；延长酶蛋白；1型二酰基甘油酰基转移酶蛋白；2型二酰基甘油酰基转移酶蛋白；3型二酰基甘油酰基转移酶蛋白；甘油-3-磷酸酰基转移酶蛋白；sn-2酰基甘油脂肪酰基转移酶蛋白；溶血磷脂酸酰基转移酶蛋白；磷脂酸磷酸酶蛋白；甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白；和/或磷脂:二酰基甘油酰基转移酶蛋白。所述一种或多种遗传修饰可以是用编码以下各项的一种或多种核酸进行的转化：Δ9去饱和酶蛋白；延长酶蛋白；1型二酰基甘油酰基转移酶蛋白；2型二酰基甘油酰基转移酶蛋白；3型二酰基甘油酰基转移酶蛋白；甘油-3-磷酸酰基转移酶蛋白；sn-2酰基甘油脂肪酰基转移酶蛋白；溶血磷脂酸酰基转移酶蛋白；磷脂酸磷酸酶蛋白；甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白；和/或磷脂:二酰基甘油酰基转移酶蛋白。
在一些实施方案中，所述转化的细胞包含降低所述细胞中天然蛋白质的活性的一种或多种遗传修饰。举例来说，所述转化的细胞可以包含降低以下各项的活性的一种或多种遗传修饰：天然的Δ9去饱和酶蛋白；天然的Δ12去饱和酶蛋白；天然的二酰基甘油酰基转移酶蛋白；天然的三酰基甘油脂酶蛋白；天然的sn-2酰基甘油脂肪酰基转移酶蛋白；天然的溶血磷脂酸酰基转移酶蛋白；天然的磷脂酸磷酸酶蛋白；天然的甘油-3-磷酸酰基转移酶蛋白；天然的甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白；和/或天然的磷脂:二酰基甘油酰基转移酶蛋白。所述一种或多种遗传修饰可以是例如敲除突变。
在一些方面，本发明涉及一种源自于本发明的转化的细胞的产品。在一些实施方案中，所述产品包含油、脂质、或三酰基甘油。所述产品可以包含硬脂酸、油酸、或亚油酸。举例来说，所述产品可以是油酸。
在一些方面，本发明涉及改变细胞的脂质含量的方法，所述方法包括转化所述细胞。所述细胞可以选自由以下各项组成的组：藻类、细菌、霉菌、真菌、植物、以及酵母，例如，所述细胞可以是酵母。举例来说，所述细胞可以是选自由以下各项组成的组的酵母：噬腺嘌呤阿克苏拉酵母、酿酒酵母、以及解脂耶氏酵母。
在一些实施方案中，所述方法包括将所述细胞用提高所述细胞中一种或多种蛋白质的活性的一种或多种核酸转化。举例来说，所述一种或多种核酸可以提高以下各项的活性：Δ9去饱和酶蛋白；延长酶蛋白；1型二酰基甘油酰基转移酶蛋白；2型二酰基甘油酰基转移酶蛋白；3型二酰基甘油酰基转移酶蛋白；甘油-3-磷酸酰基转移酶蛋白；sn-2酰基甘油脂肪酰基转移酶蛋白；溶血磷脂酸酰基转移酶蛋白；磷脂酸磷酸酶蛋白；甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白；和/或磷脂:二酰基甘油酰基转移酶蛋白。所述一种或多种核酸可以编码Δ9去饱和酶基因、延长酶基因、1型二酰基甘油酰基转移酶基因、2型二酰基甘油酰基转移酶基因、3型二酰基甘油酰基转移酶基因、甘油-3-磷酸酰基转移酶基因、sn-2酰基甘油脂肪酰基转移酶基因、溶血磷脂酸酰基转移酶基因、磷脂酸磷酸酶基因、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶基因、和/或磷脂:二酰基甘油酰基转移酶基因。
在一些实施方案中，所述方法包括将所述细胞用降低所述细胞中天然蛋白质的活性的核酸转化。举例来说，所述核酸可以降低以下各项的活性：天然的Δ9去饱和酶蛋白；天然的Δ12去饱和酶蛋白；天然的二酰基甘油酰基转移酶蛋白；天然的三酰基甘油脂酶蛋白；天然的sn-2酰基甘油脂肪酰基转移酶蛋白；天然的溶血磷脂酸酰基转移酶蛋白；天然的磷脂酸磷酸酶蛋白；天然的甘油-3-磷酸酰基转移酶蛋白；天然的甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白；和/或天然的磷脂:二酰基甘油酰基转移酶蛋白。所述核酸可以通过敲除编码天然蛋白质的基因来降低所述蛋白质的活性，例如，所述核酸可以与所述基因和/或所述基因的调节区中的核苷酸序列重组，从而破坏所述基因转录或翻译成与所述天然蛋白质具有相同水平的活性的蛋白质。
参考以下说明、附图、以及权利要求书，本发明的这些和其它特征、方面、以及优势将被更好地理解。
附图说明
图1描绘了可以被操纵以改变细胞的脂质含量或脂质组成的各种生物合成途径。
图2描绘了用于使二酰基甘油酰基转移酶DGA1基因NG66在解脂耶氏酵母菌株NS18(从ARS培养物保藏中心(ARS Culture Collection)获得，NRRL号：YB 392)中过表达的pNC243构建体的图谱。在转化之前通过PacI/NotI限制酶切消化将载体pNC243线性化。“2uori”表示来自2μm环质粒的酿酒酵母复制起点；“pMB1ori”表示来自pBR322质粒的大肠杆菌pMB1复制起点；“AmpR”表示用作氨苄西林(ampicillin)选择标记的bla基因；“PR2”表示解脂耶氏酵母GPD1启动子-931至-1；“NG66”表示通过GenScript合成的天然圆红冬孢酵母(Rhodosporidium toruloides)DGA1cDNA；“TER1”表示终止密码子之后300个碱基对的解脂耶氏酵母CYC1终止子；“PR22”表示酿酒酵母TEF1启动子-412至-1；“NG3”表示用作诺尔斯菌素(nourseothricin)选择标记的诺尔斯氏链霉菌(Streptomyces noursei)Nat1基因；“TER2”表示终止密码子之后275个碱基对的酿酒酵母CYC1终止子；并且“Sc URA3”表示用于在酵母中选择的酿酒酵母URA3营养缺陷型标记。
图3描绘了在天然Δ12去饱和酶基因缺失之前(NS18)和之后(NS419)解脂耶氏酵母菌株的C16脂肪酸和C18脂肪酸的百分比，所述脂肪酸是棕榈酸酯、棕榈油酸酯、硬脂酸酯、油酸酯、以及亚油酸酯。
图4描绘了在用编码解脂耶氏酵母Δ9去饱和酶基因的另外拷贝的核酸进行转化之前(NS18)和之后(NS441)解脂耶氏酵母菌株的C16脂肪酸和C18脂肪酸的百分比，所述脂肪酸是棕榈酸酯、棕榈油酸酯、硬脂酸酯、油酸酯、以及亚油酸酯。
图5(由图A-C组成)描绘了对包含提高或降低延长酶蛋白的活性的遗传修饰的解脂耶氏酵母细胞进行的实验。(A)在天然ELO1基因缺失之前(NS18)和之后(NS276)解脂耶氏酵母菌株的脂肪酸的百分比，所述脂肪酸是C16脂肪酸或C18脂肪酸。(B)在用编码解脂耶氏酵母ELO1基因的另外拷贝的核酸进行转化之前(NS452)和之后(NS477)解脂耶氏酵母菌株的脂肪酸的百分比，所述脂肪酸是C16脂肪酸或C18脂肪酸。(C)在用编码解脂耶氏酵母ELO1基因的另外拷贝的核酸进行转化之前(NS452)和之后(NS477)解脂耶氏酵母菌株的脂肪酸的百分比，所述脂肪酸是棕榈酸酯、棕榈油酸酯、硬脂酸酯、油酸酯、以及亚油酸酯。菌株NS452包含编码DGA1蛋白的解脂耶氏酵母DGAT2基因的另外的拷贝和编码DGA2蛋白的紫色麦角菌(Claviceps purpurea)DGAT1基因的拷贝、以及天然Δ12去饱和酶基因的缺失。
图6描绘了在将解脂耶氏酵母菌株NS418用包含来自其它生物体的Δ9去饱和酶基因的核酸转化之后所述菌株的C16脂肪酸和C18脂肪酸的百分比，所述脂肪酸是棕榈酸酯、棕榈油酸酯、硬脂酸酯、油酸酯、以及亚油酸酯，所述菌株包含天然Δ9去饱和酶基因的缺失。
图7描绘了在天然甘油酰基转移酶基因(SCT1)缺失之前(NS18)和之后(NS563)解脂耶氏酵母菌株的C16脂肪酸和C18脂肪酸的百分比，所述脂肪酸是棕榈酸酯、棕榈油酸酯、硬脂酸酯、油酸酯、以及亚油酸酯。
图8描绘了在将解脂耶氏酵母菌株NS18用包含来自各种物种的DGAT2基因的核酸转化之后所述菌株的C16脂肪酸和C18脂肪酸的百分比，所述脂肪酸是棕榈酸酯、棕榈油酸酯、硬脂酸酯、油酸酯、以及亚油酸酯。所述DGAT2基因编码DGA1蛋白。
图9描绘了在将解脂耶氏酵母菌株NS281用包含来自各种物种的DGAT1基因的核酸转化之后所述菌株的C16脂肪酸和C18脂肪酸的百分比，所述脂肪酸是棕榈酸酯、棕榈油酸酯、硬脂酸酯、油酸酯、以及亚油酸酯，所述菌株包含编码来自圆红冬孢酵母的DGA1蛋白的核酸。所述DGAT1基因编码DGA2蛋白。
图10描绘了在将解脂耶氏酵母菌株NS564用包含来自各种物种的SCT1基因的核酸转化之后所述菌株的C16脂肪酸和C18脂肪酸的百分比，所述脂肪酸是棕榈酸酯、棕榈油酸酯、硬脂酸酯、油酸酯、以及亚油酸酯，所述菌株包含天然Δ12去饱和酶基因和天然SCT1基因的缺失。
图11描绘了在天然Δ12去饱和酶基因缺失之前(NS252)和之后(NS478)噬腺嘌呤阿克苏拉酵母菌株的C16脂肪酸和C18脂肪酸的百分比，所述脂肪酸是棕榈酸酯、棕榈油酸酯、硬脂酸酯、油酸酯、以及亚油酸酯。
图12描绘了在将噬腺嘌呤阿克苏拉酵母菌株NS252用包含来自各种物种的DGAT2基因的核酸转化之后所述菌株的C16脂肪酸和C18脂肪酸的百分比，所述脂肪酸是棕榈酸酯、棕榈油酸酯、硬脂酸酯、油酸酯、以及亚油酸酯。所述DGAT2基因编码DGA1蛋白。
图13描绘了用于将解脂耶氏酵母菌株NS551工程化的策略。
图14描绘了包含Δ12去饱和酶敲除和各种延长酶基因的添加的噬腺嘌呤阿克苏拉酵母菌株的C16脂肪酸和C18脂肪酸的百分比，所述脂肪酸是C16脂肪酸或C18脂肪酸。将每一种延长酶基因添加到噬腺嘌呤阿克苏拉酵母菌株NS554中，所述菌株包含Δ12去饱和酶敲除并且被示为对照。
图15描绘了包含各种延长酶基因的噬腺嘌呤阿克苏拉酵母菌株NS554和解脂耶氏酵母菌株NS276的作为占总C16和C18脂肪酸的百分比的各种脂肪酸的百分比。噬腺嘌呤阿克苏拉酵母菌株NS554包含Δ12去饱和酶敲除突变并且解脂耶氏酵母菌株NS276包含ELO1敲除突变。
图16描绘了包含ELO1敲除和各种延长酶基因的添加的解脂耶氏酵母菌株的C16脂肪酸和C18脂肪酸的百分比，所述脂肪酸是C16脂肪酸或C18脂肪酸。将每一种延长酶基因添加到解脂耶氏酵母菌株NS276中，所述菌株包含ELO1去饱和酶敲除并且被示为对照。
图17描绘了进一步包含来自解脂耶氏酵母的ELO1基因的噬腺嘌呤阿克苏拉酵母菌株NS557的C16脂肪酸和C18脂肪酸的百分比，所述脂肪酸是C16脂肪酸或C18脂肪酸。亲本菌株噬腺嘌呤阿克苏拉酵母NS557包含Δ12去饱和酶敲除突变并且表达解脂耶氏酵母DGA1。将菌株NS557作为对照来分析，并且水平线标示该菌株的C18百分比。
图18描绘了实施例14中所述的噬腺嘌呤阿克苏拉酵母菌株NS776的包含各种链长度和饱和度水平的脂肪酸的C16脂肪酸和C18脂肪酸的百分比。
图19是示出了其中将各种遗传修饰引入到解脂耶氏酵母菌株NS18中，从而产生菌株NS987、NS988、NS991、NS992、NS993、以及NS994的顺序的流程图，所述菌株描述于实施例15中。
图20描绘了描述于实施例16中的各种解脂耶氏酵母菌株的包含各种链长度和饱和度水平的C16脂肪酸和C18脂肪酸的百分比。
图21描绘了描述于实施例16中的各种解脂耶氏酵母菌株的包含各种链长度和饱和度水平的C16脂肪酸和C18脂肪酸的百分比。此外，每一种菌株的总脂质含量被示为干细胞重％(“总脂质”)。
具体实施方式
定义
冠词“一个(a/an)”和“一种”在本文中用于指代一个/种或多于一个/种(即至少一个/种)语法对象。举例来说，“一个元件(an element)”意指一个元件或多于一个元件。
术语“活性”指的是细胞执行功能的总能力。举例来说，降低细胞中酶的活性的遗传修饰可以减少细胞中所述酶的量或降低所述酶的效率。敲除减少了细胞中蛋白质的量。或者，基因突变可以降低它的蛋白质产物的效率而对细胞中所述蛋白质的量几乎没有影响。降低酶的效率的突变可以例如通过改变一个或多个活性位点残基来影响活性位点；它们可以例如通过在空间上阻挡底物或产物来损害酶的动力学；它们可以例如通过降低正确折叠的酶的比例来影响蛋白质的折叠或动力学；它们可以例如通过防止蛋白质定位到脂质颗粒来影响蛋白质定位；或它们可以例如通过添加一个或多个蛋白质切割位点或通过添加靶向蛋白质进行蛋白水解的一个或多个残基或氨基酸序列来影响蛋白质降解。这些突变影响编码区。降低蛋白质的活性的突变相反可能会影响基因的转录或翻译。举例来说，增强子或启动子的突变可以通过减少蛋白质的表达来降低它的活性。使基因的非编码部分，如它的内含子突变或缺失也可以减少转录或翻译。此外，基因的上游调节子的突变可以影响它的蛋白质产物的活性；例如，一个或多个阻遏子的过表达可以降低蛋白质的活性，并且一个或多个激活子的敲除或突变可以类似地降低蛋白质的活性。
提高细胞中蛋白质的活性的遗传修饰可以增加所述细胞中所述蛋白质的量或提高所述蛋白质的效率(例如酶的效率)。举例来说，所述遗传修饰可以简单地将所述蛋白质的另外的拷贝插入到所述细胞中以使得所述另外的拷贝被转录并且被翻译成另外的功能蛋白。所添加的基因可以是宿主生物体天然的或来自不同的生物体。或者，使基因的非编码部分，如它的内含子突变或缺失也可以增加翻译。天然基因可以通过添加引起更多转录的新启动子来改变。类似地，可以将增强子添加到基因中以增加转录，或可以使沉默子突变或从基因中缺失以增加转录。天然基因的编码区的突变也可能提高蛋白质的活性，例如通过产生不与抑制性蛋白质或分子相互作用的蛋白质变体来实现。一个或多个激活子的过表达可以通过增加蛋白质的表达来提高蛋白质的活性，并且一个或多个阻遏子的敲除或突变可以类似地提高蛋白质的活性。
术语“生物活性部分”指的是小于全长氨基酸序列，但是表现出全长序列的至少一种活性的氨基酸序列。举例来说，二酰基甘油酰基转移酶的生物活性部分可以指的是DGA1或DGA2的具有将酰基辅酶A和二酰基甘油转化成三酰基甘油的生物活性的一个或多个结构域。蛋白质的生物活性部分包括这样的肽或多肽，其包含的氨基酸序列与所述蛋白质的氨基酸序列足够相同或源自于所述蛋白质的氨基酸序列，例如以下各项中所示的氨基酸序列：SEQ ID NO:1、3、5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39、41、43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109、111、113、115、117、119、121、123、125、127、129、131、133、135、137、139、141、143、145、147、149、151、153、155、157、或159，所述肽或多肽包括比全长蛋白质少的氨基酸，并且表现出所述蛋白质的至少一种活性。类似地，蛋白质的生物活性部分包括这样的肽或多肽，其包含的氨基酸序列与所述蛋白质的氨基酸序列足够相同或源自于所述蛋白质的氨基酸序列，例如以下各项中所示的氨基酸序列：SEQ ID NO:1、3、5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39、41、43、45、47、49、51、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109、111、113、115、117、119、121、123、125、127、129、131、133、135、137、139、141、143、145、147、149、151、153、155、157、或159，所述肽或多肽包括比全长蛋白质少的氨基酸，并且表现出所述蛋白质的至少一种活性。蛋白质的生物活性部分可以包含例如100个、101个、102个、103个、104个、105个、106个、107个、108个、109个、110个、111个、112个、113个、114个、115个、116个、117个、118个、119个、120个、121个、122个、123个、124个、125个、126个、127个、128个、129个、130个、131个、132个、133个、134个、135个、136个、137个、138个、139个、140个、141个、142个、143个、144个、145个、146个、147个、148个、149个、150个、151个、152个、153个、154个、155个、156个、157个、158个、159个、160个、161个、162个、163个、164个、165个、166个、167个、168个、169个、170个、171个、172个、173个、174个、175个、176个、177个、178个、179个、180个、181个、182个、183个、184个、185个、186个、187个、188个、189个、190个、191个、192个、193个、194个、195个、196个、197个、198个、199个、200个、201个、202个、203个、204个、205个、206个、207个、208个、209个、210个、211个、212个、213个、214个、215个、216个、217个、218个、219个、220个、221个、222个、223个、224个、225个、226个、227个、228个、229个、230个、231个、232个、233个、234个、235个、236个、237个、238个、239个、240个、241个、242个、243个、244个、245个、246个、247个、248个、249个、250个、251个、252个、253个、254个、255个、256个、257个、258个、259个、260个、261个、262个、263个、264个、265个、266个、267个、268个、269个、270个、271个、272个、273个、274个、275个、276个、277个、278个、279个、280个、281个、282个、283个、284个、285个、286个、287个、288个、289个、290个、291个、292个、293个、294个、295个、296个、297个、298个、299个、300个、301个、302个、303个、304个、305个、306个、307个、308个、309个、310个、311个、312个、313个、314个、315个、316个、317个、318个、319个、320个、321个、322个、323个、324个、325个、326个、327个、328个、329个、330个、331个、332个、333个、334个、335个、336个、337个、338个、339个、340个、341个、342个、343个、344个、345个、346个、347个、348个、349个、350个、351个、352个、353个、354个、355个、356个、357个、358个、359个、360个、361个、362个、363个、364个、365个、366个、367个、368个、369个、370个、371个、372个、373个、374个、375个、376个、377个、378个、379个、380个、381个、382个、383个、384个、385个、386个、387个、388个、389个、390个、391个、392个、393个、394个、395个、396个、397个、398个、399个、400个、401个、402个、403个、404个、405个、406个、407个、408个、409个、410个、411个、412个、413个、414个、415个、416个、417个、418个、419个、420个、421个、422个、423个、424个、425个、426个、427个、428个、429个、430个、431个、432个、433个、434个、435个、436个、437个、438个、439个、440个、441个、442个、443个、444个、445个、446个、447个、448个、449个、450个、451个、452个、453个、454个、455个、456个、457个、458个、459个、460个、461个、462个、463个、464个、465个、466个、467个、468个、469个、470个、471个、472个、473个、474个、475个、476个、477个、478个、479个、480个、481个、482个、483个、484个、485个、486个、487个、488个、489个、490个、491个、492个、493个、494个、495个、496个、497个、498个、499个、500个、501个、502个、503个、504个、505个、506个、507个、508个、509个、510个、511个、512个、513个、514个、515个、516个、517个、518个、519个、520个、521个、522个、523个、524个、525个、526个、527个、528个、529个、530个、531个、532个、533个、534个、535个、536个、537个、538个、539个、540个、541个、542个、543个、544个、545个、546个、547个、548个、549个、550个、551个、552个、553个、554个、555个、556个、557个、558个、559个、560个、561个、562个、563个、564个、565个、566个、567个、568个、569个、570个、571个、572个、573个、574个、575个、576个、577个、578个、579个、580个、581个、582个、583个、584个、585个、586个、587个、588个、589个、590个、591个、592个、593个、594个、595个、596个、597个、598个、599个、600个、601个、602个、603个、604个、605个、606个、607个、608个、609个、610个、611个、612个、613个、614个、615个、616个、617个、618个、619个、620个、621个、622个、623个、624个、625个、626个、627个、628个、629个、630个、631个、632个、633个、634个、635个、636个、637个、638个、639个、640个、641个、642个、643个、644个、645个、646个、647个、648个、649个、650个、651个、652个、653个、654个、655个、656个、657个、658个、659个、660个、661个、662个、663个、664个、665个、666个、667个、668个、669个、670个、671个、672个、673个、674个、675个、676个、677个、678个、679个、680个、681个、682个、683个、684个、685个、686个、687个、688个、689个、690个、691个、692个、693个、694个、695个、696个、697个、698个、699个、700个或更多个氨基酸。通常，生物活性部分包含具有催化活性，如用于产生硬脂酸、油酸、或亚油酸的催化活性的结构域或基序。蛋白质的生物活性部分包括蛋白质的与全长肽具有相同的活性的部分以及具有比背景更高的活性的每一个部分。举例来说，相对于全长酶，酶的生物活性部分可以具有0.1％、0.5％、1％、2％、3％、4％、5％、10％、25％、30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.5％、99.6％、99.7％、99.8％、99.9％、100％、100.1％、100.2％、100.3％、100.4％、100.5％、100.6％、100.7％、100.8％、100.9％、101％、105％、110％、115％、120％、125％、130％、135％、140％、145％、150％、160％、170％、180％、190％、200％、220％、240％、260％、280％、300％、320％、340％、360％、380％、400％或更高的活性。蛋白质的生物活性部分可以包括蛋白质的缺少使蛋白质靶向细胞隔室的结构域的部分。DGA1蛋白的生物活性部分可以是具有例如262个氨基酸的长度的多肽。
术语“DGAT1”指的是编码1型二酰基甘油酰基转移酶蛋白的基因，如编码DGA2蛋白的基因。
术语“DGAT2”指的是编码2型二酰基甘油酰基转移酶蛋白的基因，如编码DGA1蛋白的基因。
“二酰基甘油酯”、“二酰基甘油”、以及“甘油二酯”是包含甘油和两个脂肪酸的酯。
术语“二酰基甘油酰基转移酶”和“DGA”指的是催化二酰基甘油形成三酰基甘油酯的任何蛋白质。二酰基甘油酰基转移酶包括1型二酰基甘油酰基转移酶(DGA2)、2型二酰基甘油酰基转移酶(DGA1)、和3型二酰基甘油酰基转移酶(DGA3)以及催化上述反应的所有同源物。
术语“二酰基甘油酰基转移酶1型”和“1型二酰基甘油酰基转移酶”指的是DGA2和DGA2直系同源物。
术语“二酰基甘油酰基转移酶2型”和“2型二酰基甘油酰基转移酶”指的是DGA1和DGA1直系同源物。
术语“结构域”指的是蛋白质的氨基酸序列中能够独立于蛋白质的其余部分折叠成稳定的三维结构的部分。
术语“药物”指的是抑制细胞生长或增殖，从而为含有赋予对药物的抗性的基因的细胞提供选择优势的任何分子。药物包括抗生素、抗微生物剂、毒素、以及农药。
“干重”和“干细胞重”意指在相对不存在水的情况下确定的重量。举例来说，在提到含油细胞包含以干重计指定百分比的特定组分时，意指该百分比是基于在基本上所有的水已经被去除之后细胞的重量来计算的。
术语“编码”指的是包含编码区、编码区的一部分、或其互补序列的核酸。DNA和RNA这两者均可以编码基因。DNA和RNA这两者均可以编码蛋白质。
如本文所用的术语“酶”指的是可以催化化学反应的蛋白质。
术语“外源”指的是被引入到细胞中的任何物质。“外源核酸”是经由细胞膜进入细胞的核酸。外源核酸可以含有存在于细胞的天然基因组中的核苷酸序列和/或先前不存在于细胞的基因组中的核苷酸序列。外源核酸包括外源基因。“外源基因”是已经被引入到细胞中(例如通过转化/转染)的进行编码以表达RNA和/或蛋白质的核酸，并且也被称为“转基因”。包含外源基因的细胞可以被称为重组细胞，其中可以引入一个或多个另外的外源基因。相对于所转化的细胞，外源基因可以来自相同的或不同的物种。因此，外源基因可以包括相对于基因的内源拷贝，在细胞的基因组中占据不同的位置或处在不同的控制下的天然基因。外源基因在细胞中可以多于一个拷贝存在。外源基因可以作为基因组(核或质体)中的插入序列或作为游离型分子保持于细胞中。
术语“表达”指的是细胞中核酸序列或氨基酸序列(例如肽、多肽或蛋白质)的量。基因表达增加指的是该基因的转录增加。氨基酸序列、肽、多肽、或蛋白质的表达增加指的是编码所述氨基酸序列、肽、多肽、或蛋白质的核酸的翻译增加。
如本文所用的术语“基因”可以涵盖含有外显子的基因组序列，特别是编码涉及特定活性的多肽序列的多核苷酸序列。所述术语进一步涵盖了并非源自于基因组序列的合成核酸。在某些实施方案中，所述基因缺少内含子，这是因为它们是基于cDNA和蛋白质序列的已知DNA序列来合成的。在其它实施方案中，所述基因是合成的非天然的cDNA，其中密码子已经基于密码子使用被优化以在解脂耶氏酵母中表达。所述术语可以进一步包括包含上游、下游、和/或内含子核苷酸序列的核酸分子。
术语“遗传修饰”指的是转化的结果。根据定义，每一种转化均会引起遗传修饰。
如本文所用的术语“同源物”指的是(a)肽、低聚肽、多肽、蛋白质、以及酶，它们相对于所考虑的未修饰的蛋白质具有氨基酸取代、缺失和/或插入并且与作为它们的来源的未修饰的蛋白质具有相似的生物活性和功能活性；以及(b)编码具有(a)中所述的相同特征的肽、低聚肽、多肽、蛋白质、以及酶的核酸。
“诱导型启动子”是响应于特定的刺激而介导可操作连接的基因转录的启动子。
术语“整合”指的是作为细胞基因组中的插入序列，如染色体中的插入序列，包括质体基因组中的插入序列被保持在细胞中的核酸。
“可操作连接”指的是两个核酸序列，如控制序列(通常是启动子)与所连接的序列(通常是编码蛋白质的序列，也被称为编码序列)之间的功能性连接。如果启动子可以介导基因的转录，那么所述启动子与所述基因是可操作连接的。
术语“敲除突变”或“敲除”指的是防止天然基因被转录并且被翻译成功能蛋白的遗传修饰。
术语“天然”指的是在转化事件之前细胞或亲本细胞的组成。“天然基因”指的是编码并非通过转化事件引入到细胞中的蛋白质的核苷酸序列。“天然蛋白质”指的是由天然基因编码的氨基酸序列。
术语“核酸”指的是具有任何长度的核苷酸(脱氧核糖核苷酸或核糖核苷酸)的聚合形式、或其类似物。多核苷酸可以具有任何三维结构，并且可以执行任何功能。以下是多核苷酸的非限制性实例：基因或基因片段的编码区或非编码区、由连锁分析所限定的多个基因座(一个基因座)、外显子、内含子、信使RNA(mRNA)、转移RNA、核糖体RNA、核糖酶、cDNA、重组多核苷酸、支链多核苷酸、质粒、载体、分离的具有任何序列的DNA、分离的具有任何序列的RNA、核酸探针、以及引物。多核苷酸可以包含修饰的核苷酸，如甲基化的核苷酸和核苷酸类似物。如果存在的话，可以在聚合物组装之前或之后赋予对核苷酸结构的修饰。多核苷酸可以被进一步修饰，如通过与标记组分缀合。在本文提供的所有核酸序列中，U核苷酸是可与T核苷酸互换的。
首字母缩写“ORF”代表开放阅读框。
术语“亲本细胞”指的是作为细胞的亲代的每一种细胞。细胞的基因组包含亲本细胞的基因组和对亲本细胞的基因组的任何后续的遗传修饰。
如本文所用的术语“质粒”指的是与生物体的基因组DNA物理分离的环状DNA分子。质粒在被引入到宿主细胞中之前可以被线性化(在本文被称作线性化质粒)。线性化质粒可能不是自我复制的，但是可以整合到生物体的基因组DNA中并且与之一起复制。
术语“部分”指的是肽、低聚肽、多肽、蛋白质结构域、以及蛋白质。编码“蛋白质的部分”的核苷酸序列包括可以被转录和/或翻译的核苷酸序列和必须经历一个或多个重组事件以被转录和/或翻译的核苷酸序列这两者。举例来说，核酸可以包含编码选择性标记蛋白的一个或多个氨基酸的核苷酸序列。该核酸可以被工程化以与编码所述蛋白质的其余部分的一个或多个不同的核苷酸序列重组。这样的核酸可用于产生敲除突变，这是因为只有与靶序列重组才有可能重构全长选择性标记基因，而随机整合事件不可能产生可以产生功能性标记蛋白的核苷酸序列。
“启动子”是引导核酸转录的核酸控制序列。如本文所用，启动子包括靠近转录起始位点的必要核酸序列。启动子还任选地包括远端增强子或阻遏子元件，它们可以位于距转录起始位点多达数千个碱基对处。
术语“蛋白质”指的是包含氨基酸序列的分子，其中所述氨基酸是由肽键连接的。
“重组”指的是细胞、核酸、蛋白质、或载体，它已经由于外源核酸的引入或天然核酸的改变而被修饰。因此，例如，重组细胞可以表达细胞的天然(非重组)形式内不存在的基因或表达天然基因而不同于那些基因由非重组细胞表达。重组细胞可以包括但不限于编码基因产物或降低细胞中活性基因产物的水平的抑制元件，如突变、敲除、反义RNA、干扰RNA(RNAi)、或dsRNA的重组核酸。“重组核酸”是一般通过操纵核酸，例如使用聚合酶、连接酶、核酸外切酶、以及核酸内切酶而最初在体外形成的核酸，或以其它方式呈自然界中通常不存在的形式。可以产生重组核酸，例如以使两个或更多个核酸处于可操作连接。因此，为了实现本发明的目的，通过连接在自然界中通常不会连接的DNA分子而在体外形成的分离的核酸或表达载体这两者均被认为是重组的。一旦重组核酸被制备并且引入到宿主细胞或生物体中，它就可以使用宿主细胞的体内细胞机制进行复制；然而，这样的核酸一旦重组产生，尽管随后在细胞内进行复制，但为了实现本发明的目的而仍然被认为是重组的。类似地，“重组蛋白”是使用重组技术，即经由使重组核酸表达而制备的蛋白质。
术语“调节区”指的是影响基因的转录或翻译，但是不编码氨基酸序列的核苷酸序列。调节区包括启动子、操纵子、增强子、以及沉默子。
术语“基本上相同”指的是编码蛋白质的生物活性部分的核苷酸序列或氨基酸序列，所述序列与参考序列具有70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同一性。对于酶，基本上相同的序列通常保留参考序列的酶活性。举例来说，如果序列编码具有参考酶的酶活性的10％至1,000％的酶，那么所述序列与参考序列基本上相同。
“转化”指的是将核酸转移到宿主生物体或宿主生物体的基因组中，从而引起遗传稳定的遗传。含有转化的核酸片段的宿主生物体被称为“重组”、“转基因”或“转化”生物体。因此，本发明的分离的多核苷酸可以被并入重组构建体中，所述构建体通常是DNA构建体，能够引入到宿主细胞中并且在宿主细胞中复制。这样的构建体可以是载体，所述载体包括复制系统和能够使多肽编码序列在给定的宿主细胞中转录和翻译的序列。通常，表达载体包括例如处在5'端和3'端调节序列的转录控制下的一个或多个克隆基因和选择性标记。这样的载体还可以含有启动子调节区(例如控制诱导型或组成型、环境调节或发育调节的、或位置特异性表达的调节区)、转录起始位点、核糖体结合位点、转录终止位点、和/或多聚腺苷酸化信号。
术语“转化的细胞”指的是已经经历转化的细胞。因此，转化的细胞包含亲本的基因组和可遗传的遗传修饰。
术语“三酰基甘油酯”、“三酰基甘油”、“甘油三酯”以及“TAG”是包含甘油和三个脂肪酸的酯。
术语“三酰基甘油脂酶”指的是可以催化脂肪酸链从三酰基甘油中去除的任何蛋白质。三酰基甘油脂酶包括TGL3、TGL4、以及TGL3/4。
术语“载体”指的是用于使核酸可以在生物体、细胞、或细胞组分之间传播和/或转移的工具。载体包括质粒、线性DNA片段、病毒、噬菌体、前病毒、噬菌粒、转座子、以及人工染色体等，它们可能能够或可能不能自主复制或整合到宿主细胞的染色体中。
微生物工程化
A.概述
在本发明的某些实施方案中，微生物被遗传修饰以改变它的脂质组成，例如增加它的油酸含量(图1)。
基因和基因产物可以被引入微生物宿主细胞中。用于表达基因和核酸分子的合适的宿主细胞是可以在真菌科或细菌科内广泛发现的微生物宿主。合适的宿主菌株的实例包括但不限于真菌或酵母物种，如阿克苏拉酵母属(Arxula)、曲霉属(Aspegillus)、破囊壶菌属(Aurantiochytrium)、假丝酵母属(Candida)、麦角菌属(Claviceps)、隐球菌属(Cryptococcus)、小克银汉霉菌属(Cunninghamella)、汉逊酵母属(Hansenula)、克鲁维酵母属(Kluyveromyces)、白冬孢酵母属(Leucosporidiella)、油脂酵母属(Lipomyces)、被孢霉属(Mortierella)、欧加铁酵母属(Ogataea)、毕赤酵母属(Pichia)、原壁藻属(Prototheca)、根霉属(Rhizopus)、红冬孢酵母属(Rhodosporidium)、红酵母属(Rhodotorula)、酵母菌属(Saccharomyces)、裂殖酵母属(Schizosaccharomyces)、银耳属(Tremella)、丝孢酵母属(Trichosporon)、耶氏酵母属(Yarrowia)；或细菌物种，如变形菌门(proteobacteria)和放线菌类的成员，以及以下属：不动杆菌属(Acinetobacter)、节杆菌属(Arthrobacter)、短杆菌属(Brevibacterium)、食酸菌属(Acidovorax)、芽孢杆菌属(Bacillus)、梭菌纲(Clostridia)、链霉菌属(Streptomyces)、埃希氏菌属(Escherichia)、沙门氏菌属(Salmonella)、假单胞菌属(Pseudomonas)、以及棒状杆菌属(Cornyebacterium)。解脂耶氏酵母和噬腺嘌呤阿克苏拉酵母适用作宿主微生物，这是因为它们可以三酰基甘油的形式积聚它们重量的较大百分比。
含有引导外来蛋白质的高水平表达的调节序列的微生物表达系统和表达载体是本领域技术人员已知的。这些中的任一种可以用于构建嵌合基因以产生具有本发明的序列的基因产物中的任一种。然后可以经由转化技术将这些嵌合基因引入到适当的微生物中以提供酶的高水平表达。
举例来说，可以将编码酶的基因在合适的质粒中克隆，并且可以将作为宿主的上述起始亲本菌株用所得的质粒转化。这种方法可以增加编码酶的基因中的每一种的拷贝数并且因此，可以提高酶的活性。所述质粒不受特别限制，只要它使得所期望的遗传修饰可遗传到微生物的后代即可。
可用于转化合适的宿主细胞的载体或盒是本领域公知的。通常，所述载体或盒含有引导相关基因转录和翻译的序列、选择性标记、以及允许自主复制或染色体整合的序列。合适的载体包含携带转录起始控制的基因的5'端区域和控制转录终止的DNA片段的3'端区域。在某些实施方案中，这两个控制区源自于与转化的宿主细胞同源的基因，尽管应当了解的是，这样的控制区不需要源自于被选择作为产生宿主的特定物种天然的基因。
启动子、cDNA和3'UTR以及载体的其它元件可以经由克隆技术，使用从天然来源分离的片段来产生(Green和Sambrook,Molecular Cloning:A Laboratory Manual(《分子克 隆：实验室手册》),(第4版,2012)；美国专利号4,683,202(以引用的方式并入本文))。或者，可以使用已知的方法合成产生元件(Gene 164:49-53(1995))。
B.同源重组
同源重组是互补DNA序列对齐和交换同源区的能力。将含有与所靶向的基因组序列(“模板”)同源的序列的转基因DNA(“供体”)引入到生物体中，然后在相应的同源基因组序列的位点处重组到基因组中。
能够在宿主生物体中进行同源重组的能力对于在分子遗传学水平上可以进行的操作具有许多实际意义并且可用于产生可以产生所期望的产物的微生物。根据同源重组的性质，它是精确的基因靶向事件，并且因此，用相同的靶向序列产生的大多数转基因株系在表型方面将是基本上相同的，从而需要筛选少得多的转化事件。同源重组还将基因插入事件定向到宿主染色体中，从而潜在地产生优良的遗传稳定性，即使是在不存在遗传选择的情况下。由于不同的染色体基因座可能会影响基因表达，甚至是由外源启动子/UTR所引起的基因表达，因此同源重组可以是在不熟悉的基因组环境中查询基因座以及评估这些环境对基因表达的影响的方法。
使用同源重组的特别有用的遗传工程化方法是选出特异性宿主调节元件，如启动子/UTR，而以高度特异性方式驱动异源性基因表达。
由于同源重组是精确的基因靶向事件，因此它可以用于对所关注的基因或区域内的任何一个或多个核苷酸进行精确修饰，只要已经鉴定出足够的侧接区域即可。因此，同源重组可以用作对影响RNA和/或蛋白质的基因表达的调节序列进行修饰的手段。它还可以用于修饰蛋白质编码区以试图改变酶活性，如底物特异性、亲和力以及Km，从而影响宿主细胞代谢的所期望的变化。同源重组提供了操纵宿主基因组，从而引起基因靶向、基因转换、基因缺失、基因复制、基因倒置以及交换基因表达调节元件，如启动子、增强子以及3'UTR的强有力的手段。
可以通过使用含有内源性序列片段的靶向构建体来“靶向”内源性宿主细胞基因组内所关注的基因或区域来实现同源重组。这样的靶向序列可以位于所关注的基因或区域的5'端处、所关注的基因/区域的3'端处或甚至侧接所关注的基因/区域。这样的靶向构建体可以作为具有另外的载体骨架的超螺旋质粒DNA、没有载体骨架的PCR产物或线性化分子被转化到宿主细胞中。在一些情况下，可能有利的是，首先通过用限制性内切酶切割转基因DNA来暴露转基因DNA(供体DNA)内的同源序列。这个步骤可以提高重组效率并且减少不期望的事件的发生。提高重组效率的其它方法包括使用PCR产生转化转基因DNA，所述DNA含有与所靶向的基因组序列同源的线性末端。
C.载体和载体组分
鉴于本文的公开内容，可以通过为本领域技术人员所熟悉的已知技术来制备用于转化根据本发明的微生物的载体。载体通常含有一个或多个基因，其中每一个基因进行编码以使所期望的产物(基因产物)表达，并且与调节基因表达或使基因产物靶向重组细胞中的特定位置的一个或多个控制序列可操作地连接。
1.控制序列
控制序列是调节编码序列的表达或将基因产物引导到细胞中或细胞外的特定位置的核酸。调节表达的控制序列包括例如调节编码序列转录的启动子和使编码序列的转录终止的终止子。另一个控制序列是位于编码序列的末端处的编码多聚腺苷酸化信号的3'非翻译序列。将基因产物引导到特定位置的控制序列包括编码信号肽的序列，所述信号肽将与它们连接的蛋白质引导到细胞内或细胞外的特定位置。
因此，用于使基因在微生物中表达的示例性载体设计含有所期望的基因产物(例如选择性标记或酶)的编码序列，所述编码序列与在酵母中具有活性的启动子可操作地连接。或者，如果载体不含与所关注的编码序列可操作地连接的启动子，那么可以将所述编码序列转化到细胞中，以使它在载体整合时与内源性启动子可操作地连接。
用于表达基因的启动子可以是与该基因天然连接的启动子或不同的启动子。
启动子一般可以被表征为组成型或诱导型。组成型启动子一般在所有时间(或在细胞生命周期中的某些时间)具有相同水平的驱动表达的活性或功能。相反，诱导型启动子仅响应于刺激而具有活性(或致使其无活性)或显著上调或下调。这两种类型的启动子均可以应用于本发明的方法中。可用于本发明中的诱导型启动子包括响应于刺激而介导可操作连接的基因转录的那些启动子，所述刺激诸如外源性提供的小分子、温度(热或冷)、培养基中缺少氮等。合适的启动子可以激活基本上沉默的基因转录或上调，例如显著上调以低水平转录的可操作连接的基因的转录。
终止区控制序列的包括是任选的，并且如果使用的话，则选择主要是方便的序列，这是因为终止区是相对可互换的。终止区可以是转录起始区(启动子)天然的、可以是所关注的DNA序列天然的、或可以是可从另外的来源获得的(参见例如Chen和Orozco,NucleicAcids Research 16:8411(1988))。
2.基因和密码子优化
通常，基因包括启动子、编码序列以及终止控制序列。当通过重组DNA技术进行组装时，基因可以被称为表达盒，并且可以由限制性位点侧接以便于插入到用于将重组基因引入宿主细胞中的载体中。表达盒可以由来自基因组或其它核酸靶标的DNA序列侧接以有助于表达盒通过同源重组稳定整合到基因组中。或者，载体和它的表达盒可以保持未整合(例如游离体)，在这种情况下，载体通常包括能够提供载体DNA的复制的复制起点。
载体上存在的常见基因是编码蛋白质的基因，所述基因的表达允许含有所述蛋白质的重组细胞与不表达所述蛋白质的细胞相区别。这样的基因和它相应的基因产物被称作选择性标记或选择标记。多种选择性标记中的任一种可以应用于可用于转化本发明的生物体的转基因构建体中。
为了使重组蛋白最佳地表达，有益的是，使用产生具有由待转化的宿主细胞最佳使用的密码子的mRNA的编码序列。因此，转基因的适当表达可能需要转基因的密码子使用与其中所述转基因正被表达的生物体的特异性密码子偏倚性相匹配。这种效应潜在的精确机制有许多，但是包括可用的氨基酰化tRNA池与细胞中正合成的蛋白质的适当平衡，联同当这一需要被满足时转基因信使RNA(mRNA)的更高效的翻译。当转基因中的密码子使用没有经过优化时，可用的tRNA池不足以允许转基因mRNA的高效翻译，从而导致核糖体失速和终止以及转基因mRNA可能的不稳定性。
C.转化
可以通过任何合适的技术转化细胞，所述技术包括例如生物射弹法、电穿孔法、玻璃珠转化法以及碳化硅晶须转化法。在本发明中可以使用用于将转基因引入到微生物中的任何方便的技术。转化可以通过例如以下方法来实现：D.M.Morrison(Methods inEnzymology 68:326(1979))的方法；通过用氯化钙提高接受体细胞对DNA的通透性而实现的方法(Mandel和Higa,J.Molecular Biology,53:159(1970))等。
转基因在含油酵母(例如解脂耶氏酵母)中表达的实例可以见于文献(Bordes等,J.Microbiological Methods,70:493(2007)；Chen等,Applied Microbiology&Biotechnology 48:232(1997))中。外源基因在诸如大肠杆菌的细菌中表达的实例是公知的(Green和Sambrook,MolecularCloning:A LaboratoryManual(《分子克隆：实验室手 册》),(第4版,2012))。
用于转化根据本发明的微生物的载体可以通过为本领域技术人员所熟悉的已知技术来制备。在一个实施方案中，用于使基因在微生物中表达的示例性载体设计含有编码酶的基因，所述基因与在微生物中具有活性的启动子可操作地连接。或者，如果载体不含与所关注的基因可操作连接的启动子，那么可以将所述基因转化到细胞中以使它在载体整合时与天然启动子可操作地连接。所述载体还可以含有编码蛋白质的第二基因。任选地，一个或这两个基因后面是含有多聚腺苷酸化信号的3'非翻译序列。编码这两个基因的表达盒可以物理方式连接于载体中或单独的载体上。还可以使用微生物的共同转化，其中同时使用不同的载体分子来转化细胞(Protist155:381-93(2004))。转化的细胞可以任选地基于在抗生素存在下生长的能力或其它选择性标记在其中缺少抗性盒的细胞不会生长的条件下来选择。
示例性细胞、核酸、以及方法
A.转化的细胞
在一些实施方案中，转化的细胞是原核细胞，如细菌细胞。在一些实施方案中，所述细胞是真核细胞，如哺乳动物细胞、酵母细胞、丝状真菌细胞、天然生物细胞、藻类细胞、禽类细胞、植物细胞、或昆虫细胞。在一些实施方案中，所述细胞是酵母。本领域技术人员将认识到的是，丝状真菌的许多形式产生酵母样生长，并且本文中酵母的定义涵盖了这样的细胞。
所述细胞可以选自由以下各项组成的组：阿克苏拉酵母属、曲霉属、破囊壶菌属、假丝酵母属、麦角菌属、隐球菌属、小克银汉霉菌属、地霉属(Geotrichum)、汉逊酵母属、克鲁维酵母属、柯达酵母属(Kodamaea)、白冬孢酵母属、油脂酵母属、被孢霉属、欧加铁酵母属、毕赤酵母属、原壁藻属、根霉属、红冬孢酵母属、红酵母属、酵母菌属、裂殖酵母属、银耳属、丝孢酵母属、威克汉姆酵母属(Wickerhamomyces)、以及耶氏酵母属。
在一些实施方案中，所述细胞选自由以下各项组成的组：噬腺嘌呤阿克苏拉酵母、黑曲霉(Aspergillus niger)、米曲霉(Aspergillus orzyae)、土曲霉(Aspergillusterreus)、裂殖壶菌(Aurantiochytrium limacinum)、产朊假丝酵母(Candida utilis)、紫色麦角菌、白色隐球菌(Cryptococcus albidus)、弯曲隐球菌(Cryptococcus curvatus)、拉氏隐球菌(Cryptococcus ramirezgomezianus)、地生隐球菌(Cryptococcus terreus)、威氏隐球菌(Cryptococcus wieringae)、刺孢小克银汉霉菌(Cunninghamellaechinulata)、日本小克银汉霉菌(Cunninghamella japonica)、发酵地霉(Geotrichumfermentans)、多形汉逊酵母(Hansenula polymorpha)、乳酸克鲁维酵母(Kluyveromyceslactis)、马克斯克鲁维酵母(Kluyveromyces marxianus)、奥默柯达酵母(Kodamaeaohmeri)、克里替弗拉白冬孢酵母(Leucosporidiella creatinivora)、产油油脂酵母(Lipomyces lipofer)、斯达氏油脂酵母(Lipomyces starkeyi)、子囊菌油脂酵母(Lipomyces tetrasporus)、黄褐色被孢霉(Mortierella isabellina)、高山被孢霉、多形欧加铁酵母(Ogataea polymorpha)、西弗毕赤酵母(Pichia ciferrii)、季也蒙毕赤酵母(Pichia guilliermondii)、巴斯德毕赤酵母(Pichia pastoris)、树干毕赤酵母(Pichiastipites)、饶氏原壁藻(Prototheca zopfii)、少根根霉(Rhizopus arrhizus)、巴布维红冬孢酵母(Rhodosporidium babjevae)、圆红冬孢酵母、沼泽生红冬孢酵母(Rhodosporidium paludigenum)、粘红酵母(Rhodotorula glutinis)、胶红酵母(Rhodotorula mucilaginosa)、酿酒酵母、粟酒裂殖酵母(Schizosaccharomyces pombe)、恩氏银耳(Tremella enchepala)、皮状丝孢酵母(Trichosporon cutaneum)、发酵丝孢酵母(Trichosporon fermentans)、西弗威克汉姆酵母(Wickerhamomyces ciferrii)、以及解脂耶氏酵母。
在某些实施方案中，所述细胞是酿酒酵母、解脂耶氏酵母、或噬腺嘌呤阿克苏拉酵母。
在一些实施方案中，所述细胞是酵母、真菌、或酵母样藻类。所述细胞可以选自破囊壶菌(破囊壶菌属)和无叶绿素单细胞藻类(原壁藻属)。
在某些实施方案中，转化的细胞包含至少50％、51％、52％、53％、54％、55％、56％、57％、58％、59％、60％、61％、62％、63％、64％、65％、66％、67％、68％、69％、70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、或更多的脂质，所述脂质是通过干细胞重量％所测量。在一些实施方案中，转化的细胞包含作为占细胞中总C16和C18脂肪酸的百分比的至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％或更高的浓度的C18脂肪酸。在一些实施方案中，转化的细胞包含作为占细胞中总C16和C18脂肪酸的百分比的至少50％、51％、52％、53％、54％、55％、56％、57％、58％、59％、60％、61％、62％、63％、64％、65％、66％、67％、68％、69％、70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％或更高的浓度的油酸。
B.用于提高蛋白质活性的核酸和方法
本发明的基因可以包含保守取代、缺失、和/或插入，而仍编码具有活性的蛋白质。举例来说，密码子可以针对特定的宿主细胞经过优化；不同的密码子可以被取代以为了方便起见，以引入限制性位点或形成最佳的PCR引物；或密码子可以被取代以实现另外的目的。类似地，核苷酸序列可以被改变以形成保守氨基酸取代、缺失、和/或插入。
蛋白质可以包含保守取代、缺失、和/或插入，而仍维持活性。保守取代表是本领域公知的(Creighton,Proteins(《蛋白质》)(第2版,1992))。
氨基酸取代、缺失和/或插入可以容易地使用重组DNA操纵技术来产生。用于操纵DNA序列以产生蛋白质的取代、插入或缺失变体的方法是本领域公知的。这些方法包括M13诱变、T7-Gen体外诱变(俄亥俄州克利夫兰的USB公司(USB,Cleveland,OH))、Quick Change定点诱变(加利福尼亚州圣地亚哥的Stratagene公司(Stratagene,San Diego,CA))、PCR介导的定点诱变、以及其它定点诱变方案。
为了确定两个氨基酸序列或两个核酸序列的同一性百分比，可以将所述序列比对以实现最佳比较的目的(例如，可以将空位引入到第一氨基酸序列或核酸序列和第二氨基酸序列或核酸序列中的一个或这两者中以进行最佳比对并且为了比较目的，不相同的序列可以被忽略)。为了比较目的所比对的参考序列的长度可以是参考序列的长度的至少95％。然后可以比较相应氨基酸位置或核苷酸位置处的氨基酸残基或核苷酸。当第一序列中的位置与第二序列中的相应位置由相同的氨基酸残基或核苷酸占据时，则所述分子在该位置处是相同的(如本文所用的氨基酸或核酸“同一性”等同于氨基酸或核酸“同源性”)。两个序列之间的同一性百分比是所述序列共有的相同位置的数目的函数，将需要被引入以将这两个序列进行最佳比对的空位数目和每一个空位的长度考虑在内。
比较序列以及确定两个序列之间的同一性百分比可以使用数学算法来实现。在一个实施方案中，两个氨基酸序列之间的同一性百分比可以使用已经被并入到GCG软件包(可在http://www.gcg.com获取)中的GAP程序中的Needleman和Wunsch(J.Molecular Biology48:444-453(1970))算法，使用Blosum 62矩阵或PAM250矩阵、以及16、14、12、10、8、6、或4的空位权重和1、2、3、4、5、或6的长度权重来确定。在又另一个实施方案中，两个核苷酸序列之间的同一性百分比可以使用GCG软件包(可在http://www.gcg.com获取)中的GAP程序，使用NWSgapdna.CMP矩阵以及40、50、60、70、或80的空位权重和1、2、3、4、5、或6的长度权重来确定。在另一个实施方案中，两个氨基酸序列或核苷酸序列之间的同一性百分比可以使用已经被并入到ALIGN程序(2.0版或2.0U版)中的E.Meyers和W.Miller的算法(ComputerApplications in the Biosciences 4:11-17(1988))，使用PAM120权重残基表、12的空位长度罚分以及4的空位罚分来确定。
可以用于确定两个序列之间的同一性的示例性计算机程序包括但不限于BLAST程序的套件，例如BLASTN、MEGABLAST、BLASTX、TBLASTN、TBLASTX、以及BLASTP；以及Clustal程序的套件，例如ClustalW、ClustalX、以及Clustal Omega。
当相对于基因库DNA序列和其它公共数据库中的核酸序列来评价给定的核酸序列时，通常使用BLASTN程序来进行序列搜索。BLASTX程序有效用于针对基因库蛋白质序列和其它公共数据库中的氨基酸序列搜索已经在所有阅读框中被翻译的核酸序列。
比对所选择的序列以确定两个或更多个序列之间的“同一性％”是使用例如CLUSTAL-W程序进行的。
“编码序列”或“编码区”指的是具有在表达序列时产生蛋白质产物，如氨基酸或多肽所必需的序列信息的核酸分子。编码序列可以包含翻译区内的非翻译序列(包括内含子或5'或3'非翻译区)和/或由所述非翻译序列组成，或可以缺少这样的居间非翻译序列(例如，如cDNA中那样)。
在整个说明书中用于指代包含核苷酸序列和/或由核苷酸序列组成的核酸的缩写是常规的单字母缩写。因此，当被包括在核酸中时，天然存在的编码核苷酸缩写如下：腺嘌呤(A)、鸟嘌呤(G)、胞嘧啶(C)、胸腺嘧啶(T)以及尿嘧啶(U)。此外，除非另外说明，否则本文所呈现的核酸序列是按5'→3'方向。
如本文所用的术语“互补”和其衍生词用于指核酸根据A与T或U配对以及C与G配对的公知规则进行配对。互补可以是“部分的”或“完全的”。在部分互补中，只有一些核酸碱基根据碱基配对规则匹配；而在完全或全部互补中，所有碱基根据配对规则均匹配。核酸链之间的互补程度可以对核酸链之间杂交的效率和强度有显著的影响，如本领域公知的那样。所述杂交的效率和强度取决于检测方法。
氨基酸序列和核苷酸序列可以源自于具有高的天然水平的脂质积聚的含油生物体。(Bioresource Technology 144:360-69(2013)；Progress Lipid Research 52:395-408(2013)；Applied Microbiology&Biotechnology90:1219-27(2011)；European JournalLipid Science&Technology113:1031-51(2011)；Food Technology&Biotechnology 47:215-20(2009)；Advances Applied Microbiology 51:1-51(2002)；Lipids 11:837-44(1976))。具有所报道的约50％和更高的脂质含量的生物体列表示于表1中。圆红冬孢酵母和斯达氏油脂酵母具有最高的脂质含量。
表1
具有所报道的约50％和高于50％的脂质含量的含油真菌的列表
蛋白质的活性可以通过使所述蛋白质过表达来提高。可以使用多种遗传修饰使蛋白质在细胞中过表达。在一些实施方案中，所述遗传修饰增加天然蛋白质的表达。可以通过修饰编码蛋白质的基因的上游转录调节子，例如通过增加转录激活子的表达或减少转录阻遏子的表达来使天然蛋白质过表达。或者，可以通过与外源核酸重组将天然基因的启动子用组成型活性或诱导型启动子取代。
在一些实施方案中，提高蛋白质的活性的遗传修饰包括用包含编码蛋白质的基因的核酸转化。所述基因可以是细胞天然的或来自不同的物种。在某些实施方案中，所述基因是可遗传到转化细胞的后代的。在一些实施方案中，所述基因是可遗传的，这是因为它存在于质粒上。在某些实施方案中，所述基因是可遗传的，这是因为它整合到转化的细胞的基因组中。
1.提高Δ9去饱和酶的活性
在一些方面，本发明涉及一种转化的细胞，所述细胞包含遗传修饰，其中所述遗传修饰提高所述细胞中Δ9去饱和酶蛋白的活性。所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用提高所述细胞中Δ9去饱和酶蛋白的活性的核酸转化。
所述核酸可以编码Δ9去饱和酶基因。在一些实施方案中，所述基因是Δ9。在一些实施方案中，所述基因来自噬腺嘌呤阿克苏拉酵母、密粘褶菌(Gloeophyllum trabeum)、花药黑粉菌(Microbotryum violaceum)、禾柄锈菌(Puccinia graminis)、圆红冬孢酵母、粘红酵母、禾本红酵母(Rhodotorula graminis)、或解脂耶氏酵母。所述基因可以来自噬腺嘌呤阿克苏拉酵母或禾柄锈菌。
在一些实施方案中，所述核酸包含核苷酸序列，所述核苷酸序列与以下各项中所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:4；SEQ ID NO:8；SEQ ID NO:10；SEQ ID NO:12；SEQ IDNO:14；SEQ ID NO:16；SEQ ID NO:112；或SEQ ID NO:114。所述核酸可以包含以下各项中所示的核苷酸序列：SEQ ID NO:4；SEQ ID NO:8；SEQ ID NO:10；SEQ ID NO:12；SEQ ID NO:14；SEQ ID NO:16；SEQ ID NO:112；或SEQ ID NO:114。在一些实施方案中，所述核酸包含核苷酸序列，所述核苷酸序列与SEQ ID NO:8或SEQ ID NO:14中所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性。所述核酸可以包含SEQ ID NO:8或SEQ ID NO:14中所示的核苷酸序列。
在一些实施方案中，所述核酸编码氨基酸序列，所述氨基酸序列与以下各项中所示的序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:3；SEQ ID NO:7；SEQ IDNO:9；SEQ ID NO:11；SEQ ID NO:13；SEQ ID NO:15；SEQ ID NO:111；或SEQ ID NO:113、或其生物活性部分。所述核酸可以编码以下各项中所示的氨基酸序列：SEQ ID NO:3；SEQ IDNO:7；SEQ ID NO:9；SEQ ID NO:11；SEQ ID NO:13；SEQ ID NO:15；SEQ ID NO:111；或SEQID NO:113。在一些实施方案中，所述核酸编码氨基酸序列，所述氨基酸序列与以下各项中所示的序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:7或SEQ ID NO:13、或其生物活性部分。所述核酸可以编码SEQ ID NO:7或SEQ ID NO:13中所示的氨基酸序列。
包含编码Δ9去饱和酶蛋白的基因的核酸可以包含以下各项中所示的核苷酸序列：SEQ ID NO:4；SEQ ID NO:8；SEQ ID NO:10；SEQ ID NO:12；SEQ ID NO:14；SEQ ID NO:16；SEQ ID NO:112；或SEQ ID NO:114。在其它实施方案中，所述基因与以下各项基本上相同：SEQ ID NO:4；SEQ ID NO:8；SEQ ID NO:10；SEQ ID NO:12；SEQ ID NO:14；SEQ ID NO:16；SEQ ID NO:112；或SEQ ID NO:114，并且所述核苷酸序列编码保留由以下各项编码的蛋白质的Δ9去饱和酶活性的蛋白质，但是在核苷酸序列上，例如由于天然等位基因变异或诱变而不同：SEQ ID NO:3；SEQ ID NO:7；SEQ ID NO:9；SEQ ID NO:11；SEQ ID NO:13；SEQ IDNO:15；SEQ ID NO:111；或SEQ ID NO:113。
Δ9去饱和酶蛋白可以具有以下各项中所示的氨基酸序列：SEQ ID NO:3；SEQ IDNO:7；SEQ ID NO:9；SEQ ID NO:11；SEQ ID NO:13；SEQ ID NO:15；SEQ ID NO:111；或SEQID NO:113。在其它实施方案中，Δ9去饱和酶蛋白与以下各项基本上相同：SEQ ID NO:3；SEQ ID NO:7；SEQ ID NO:9；SEQ ID NO:11；SEQ ID NO:13；SEQ ID NO:15；SEQ ID NO:111；或SEQ ID NO:113，并且保留以下各项的蛋白质的功能活性，但是在氨基酸序列上，例如由于天然等位基因变异或诱变而不同：SEQ ID NO:3；SEQ ID NO:7；SEQ ID NO:9；SEQ ID NO:11；SEQ ID NO:13；SEQ ID NO:15；SEQ ID NO:111；或SEQ ID NO:113。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：延长酶、二酰基甘油酰基转移酶、甘油-3-磷酸酰基转移酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或磷脂:二酰基甘油酰基转移酶。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸酰基转移酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。举例来说，所述转化的细胞可以包含(1)由用编码外源Δ9去饱和酶蛋白的核酸转化组成的遗传修饰；以及(2)天然Δ9去饱和酶基因中的敲除突变。
2.提高延长酶的活性
在一些方面，本发明涉及一种转化的细胞，所述细胞包含遗传修饰，其中所述遗传修饰提高所述细胞中延长酶蛋白的活性。所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用提高所述细胞中延长酶蛋白的活性的核酸转化。
所述核酸可以编码延长酶基因。在一些实施方案中，所述基因是ELO1或ELO2。在一些实施方案中，所述基因来自噬腺嘌呤阿克苏拉酵母、褐家鼠(Rattus norvegicus)、酿酒酵母或解脂耶氏酵母。
在一些实施方案中，所述核酸包含核苷酸序列，所述核苷酸序列与以下各项中所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:6；SEQ ID NO:108；SEQ ID NO:156；SEQ ID NO:158；或SEQ ID NO:160。所述核酸可以包含以下各项中所示的核苷酸序列：SEQ ID NO:6；SEQ IDNO:108；SEQ ID NO:156；SEQ ID NO:158；或SEQ ID NO:160。
在一些实施方案中，所述核酸编码氨基酸序列，所述氨基酸序列与以下各项中所示的序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:5；SEQ ID NO:107；SEQID NO:155；SEQ ID NO:157；或SEQ ID NO:159、或其生物活性部分。所述核酸可以编码以下各项中所示的氨基酸序列：SEQ ID NO:5；SEQ ID NO:107；SEQ ID NO:155；SEQ ID NO:157；或SEQ ID NO:159。
包含编码延长酶蛋白的基因的核酸可以包含以下各项中所示的核苷酸序列：SEQID NO:6；SEQ ID NO:108；SEQ ID NO:156；SEQ ID NO:158；或SEQ ID NO:160。在其它实施方案中，所述基因与以下各项基本上相同：SEQ ID NO:6；SEQ ID NO:108；SEQ ID NO:156；SEQ ID NO:158；或SEQ ID NO:160，并且所述核苷酸序列编码保留由以下各项编码的蛋白质的延长酶活性的蛋白质，但是在核苷酸序列上，例如由于天然等位基因变异或诱变而不同：SEQ ID NO:5；SEQ ID NO:107；SEQ ID NO:155；SEQ ID NO:157；或SEQ ID NO:159。
延长酶蛋白可以具有以下各项中所示的氨基酸序列：SEQ ID NO:5；SEQ ID NO:107；SEQ ID NO:155；SEQ ID NO:157；或SEQ ID NO:159。在其它实施方案中，延长酶蛋白与以下各项基本上相同：SEQ ID NO:5；SEQ ID NO:107；SEQ ID NO:155；SEQ ID NO:157；或SEQ ID NO:159，并且保留以下各项的蛋白质的功能活性，但是在氨基酸序列上，例如由于天然等位基因变异或诱变而不同：SEQ ID NO:5；SEQ ID NO:107；SEQ ID NO:155；SEQ IDNO:157；或SEQ ID NO:159。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、二酰基甘油酰基转移酶、甘油-3-磷酸酰基转移酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或磷脂:二酰基甘油酰基转移酶。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸酰基转移酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。举例来说，所述转化的细胞可以包含(1)提高延长酶蛋白的活性的遗传修饰；以及(2)降低天然Δ12去饱和酶基因的活性的遗传修饰。类似地，所述转化的细胞可以包含(1)提高延长酶蛋白的活性的遗传修饰；以及(2)提高二酰基甘油酰基转移酶蛋白的活性的遗传修饰。
3.提高酰基转移酶的活性
在一些方面，本发明涉及一种转化的细胞，所述细胞包含遗传修饰，其中所述遗传修饰提高所述细胞中酰基转移酶蛋白的活性。
a.提高1型二酰基甘油酰基转移酶的活性
在一些实施方案中，所述酰基转移酶蛋白是1型二酰基甘油酰基转移酶蛋白。所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用提高所述细胞中1型二酰基甘油酰基转移酶蛋白的活性的核酸转化。
所述核酸可以编码1型二酰基甘油酰基转移酶基因。在一些实施方案中，所述基因是DGAT1。在一些实施方案中，所述基因来自噬腺嘌呤阿克苏拉酵母、解脂耶氏酵母、圆红冬孢酵母、斯达氏油脂酵母、土曲霉、紫色麦角菌、蝗绿僵菌(Metarhizium acridum)、冬虫夏草(Ophiocordyceps sinensis)、三角褐指藻(Phaeodactylum tricornutum)、季也蒙毕赤酵母、禾本红酵母、圆红冬孢酵母、绿木霉(Trichoderma virens)、以及球毛壳霉(Chaetomium globosum)。举例来说，所述基因可以来自紫色麦角菌。
在一些实施方案中，所述核酸包含核苷酸序列，所述核苷酸序列与以下各项中所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:32；SEQ ID NO:34；SEQ ID NO:36；SEQ ID NO:38；SEQ IDNO:40；SEQ ID NO:94；SEQ ID NO:98；SEQ ID NO:102；SEQ ID NO:104；SEQ ID NO:144；SEQID NO:146；SEQ ID NO:148；或SEQ ID NO:150。所述核酸可以包含以下各项中所示的核苷酸序列：SEQ ID NO:32；SEQ ID NO:34；SEQ ID NO:36；SEQ ID NO:38；SEQ ID NO:40；SEQID NO:94；SEQ ID NO:98；SEQ ID NO:102；SEQ ID NO:104；SEQ ID NO:144；SEQ ID NO:146；SEQ ID NO:148；或SEQ ID NO:150。在一些实施方案中，所述核酸包含核苷酸序列，所述核苷酸序列与SEQ ID NO:38中所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性。所述核酸可以包含SEQ IDNO:38中所示的核苷酸序列。
在一些实施方案中，所述核酸编码氨基酸序列，所述氨基酸序列与以下各项中所示的序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:31；SEQ ID NO:33；SEQID NO:35；SEQ ID NO:37；SEQ ID NO:39；SEQ ID NO:93；SEQ ID NO:97；SEQ ID NO:101；SEQ ID NO:103；SEQ ID NO:143；SEQ ID NO:145；SEQ ID NO:147；或SEQ ID NO:149、或其生物活性部分。所述核酸可以编码以下各项中所示的氨基酸序列：SEQ ID NO:31；SEQ IDNO:33；SEQ ID NO:35；SEQ ID NO:37；SEQ ID NO:39；SEQ ID NO:93；SEQ ID NO:97；SEQ IDNO:101；SEQ ID NO:103；SEQ ID NO:143；SEQ ID NO:145；SEQ ID NO:147；或SEQ ID NO:149。在一些实施方案中，所述核酸编码氨基酸序列，所述氨基酸序列与SEQ ID NO:37或其生物活性部分中所示的序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性。所述核酸可以编码SEQ ID NO:37中所示的氨基酸序列。
包含编码1型二酰基甘油酰基转移酶蛋白的基因的核酸可以包含以下各项中所示的核苷酸序列：SEQ ID NO:32；SEQ ID NO:34；SEQ ID NO:36；SEQ ID NO:38；SEQ ID NO:40；SEQ ID NO:94；SEQ ID NO:98；SEQ ID NO:102；SEQ ID NO:104；SEQ ID NO:144；SEQ IDNO:146；SEQ ID NO:148；或SEQ ID NO:150。在其它实施方案中，所述基因与以下各项基本上相同：SEQ ID NO:32；SEQ ID NO:34；SEQ ID NO:36；SEQ ID NO:38；SEQ ID NO:40；SEQID NO:94；SEQ ID NO:98；SEQ ID NO:102；SEQ ID NO:104；SEQ ID NO:144；SEQ ID NO:146；SEQ ID NO:148；或SEQ ID NO:150，并且所述核苷酸序列编码保留由以下各项编码的蛋白质的二酰基甘油酰基转移酶活性的蛋白质，但是在核苷酸序列上，例如由于天然等位基因变异或诱变而不同：SEQ ID NO:31；SEQ ID NO:33；SEQ ID NO:35；SEQ ID NO:37；SEQID NO:39；SEQ ID NO:93；SEQ ID NO:97；SEQ ID NO:101；SEQ ID NO:103；SEQ ID NO:143；SEQ ID NO:145；SEQ ID NO:147；或SEQ ID NO:149。
1型二酰基甘油酰基转移酶蛋白可以具有以下各项中所示的氨基酸序列：SEQ IDNO:31；SEQ ID NO:33；SEQ ID NO:35；SEQ ID NO:37；SEQ ID NO:39；SEQ ID NO:93；SEQ IDNO:97；SEQ ID NO:101；SEQ ID NO:103；SEQ ID NO:143；SEQ ID NO:145；SEQ ID NO:147；或SEQ ID NO:149。在其它实施方案中，1型二酰基甘油酰基转移酶蛋白与以下各项基本上相同：SEQ ID NO:31；SEQ ID NO:33；SEQ ID NO:35；SEQ ID NO:37；SEQ ID NO:39；SEQ IDNO:93；SEQ ID NO:97；SEQ ID NO:101；SEQ ID NO:103；SEQ ID NO:143；SEQ ID NO:145；SEQ ID NO:147；或SEQ ID NO:149，并且保留以下各项的蛋白质的功能活性，但是在氨基酸序列上，例如由于天然等位基因变异或诱变而不同：SEQ ID NO:31；SEQ ID NO:33；SEQ IDNO:35；SEQ ID NO:37；SEQ ID NO:39；SEQ ID NO:93；SEQ ID NO:97；SEQ ID NO:101；SEQID NO:103；SEQ ID NO:143；SEQ ID NO:145；SEQ ID NO:147；或SEQ ID NO:149。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、延长酶、二酰基甘油酰基转移酶、甘油-3-磷酸酰基转移酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或磷脂:二酰基甘油酰基转移酶。举例来说，所述转化的细胞可以包含提高DGA1蛋白的活性的遗传修饰和提高DGA2蛋白的活性的遗传修饰。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸酰基转移酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。举例来说，所述转化的细胞可以包含(1)由用编码外源DGA2蛋白的核酸转化组成的遗传修饰；以及(2)天然DGAT1基因中的敲除突变。类似地，所述转化的细胞可以包含(1)由用编码外源DGA2蛋白的核酸转化组成的遗传修饰；以及(2)降低天然Δ12去饱和酶蛋白的活性的遗传修饰。
b.提高2型二酰基甘油酰基转移酶的活性
在一些实施方案中，所述酰基转移酶蛋白是2型二酰基甘油酰基转移酶蛋白。所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用提高所述细胞中2型二酰基甘油酰基转移酶蛋白的活性的核酸转化。
所述核酸可以编码2型二酰基甘油酰基转移酶基因。在一些实施方案中，所述基因是DGAT2。在一些实施方案中，所述基因来自土曲霉、裂殖壶菌、噬腺嘌呤阿克苏拉酵母、紫色麦角菌、密粘褶菌、斯达氏油脂酵母、花药黑粉菌、三角褐指藻、季也蒙毕赤酵母、禾柄锈菌、双倒卵形红冬孢酵母(Rhodosporidium diobovatum)、圆红冬孢酵母、禾本红酵母、或解脂耶氏酵母。所述基因可以来自解脂耶氏酵母。
在一些实施方案中，所述核酸包含核苷酸序列，所述核苷酸序列与以下各项中所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:20；SEQ ID NO:22；SEQ ID NO:24；SEQ ID NO:26；SEQ IDNO:28；SEQ ID NO:30；SEQ ID NO:52；SEQ ID NO:96；SEQ ID NO:100；SEQ ID NO:128；SEQID NO:130；SEQ ID NO:132；SEQ ID NO:134；SEQ ID NO:136；SEQ ID NO:138；SEQ ID NO:140；或SEQ ID NO:142。所述核酸可以包含以下各项中所示的核苷酸序列：SEQ ID NO:20；SEQ ID NO:22；SEQ ID NO:24；SEQ ID NO:26；SEQ ID NO:28；SEQ ID NO:30；SEQ ID NO:52；SEQ ID NO:96；SEQ ID NO:100；SEQ ID NO:128；SEQ ID NO:130；SEQ ID NO:132；SEQID NO:134；SEQ ID NO:136；SEQ ID NO:138；SEQ ID NO:140；或SEQ ID NO:142。在一些实施方案中，所述核酸包含核苷酸序列，所述核苷酸序列与SEQ ID NO:20中所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性。所述核酸可以包含SEQ ID NO:20中所示的核苷酸序列。
在一些实施方案中，所述核酸编码氨基酸序列，所述氨基酸序列与以下各项中所示的序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:19；SEQ ID NO:21；SEQID NO:23；SEQ ID NO:25；SEQ ID NO:27；SEQ ID NO:29；SEQ ID NO:51；SEQ ID NO:95；SEQID NO:99；SEQ ID NO:127；SEQ ID NO:129；SEQ ID NO:131；SEQ ID NO:133；SEQ ID NO:135；SEQ ID NO:137；SEQ ID NO:139；或SEQ ID NO:141、或其生物活性部分。所述核酸可以编码以下各项中所示的氨基酸序列：SEQ ID NO:19；SEQ ID NO:21；SEQ ID NO:23；SEQ IDNO:25；SEQ ID NO:27；SEQ ID NO:29；SEQ ID NO:51；SEQ ID NO:95；SEQ ID NO:99；SEQ IDNO:127；SEQ ID NO:129；SEQ ID NO:131；SEQ ID NO:133；SEQ ID NO:135；SEQ ID NO:137；SEQ ID NO:139；或SEQ ID NO:141。在一些实施方案中，所述核酸编码氨基酸序列，所述氨基酸序列与SEQ ID NO:19或其生物活性部分中所示的序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性。所述核酸可以编码SEQ ID NO:19中所示的氨基酸序列。
包含编码2型二酰基甘油酰基转移酶蛋白的基因的核酸可以包含以下各项中所示的核苷酸序列：SEQ ID NO:20；SEQ ID NO:22；SEQ ID NO:24；SEQ ID NO:26；SEQ ID NO:28；SEQ ID NO:30；SEQ ID NO:52；SEQ ID NO:96；SEQ ID NO:100；SEQ ID NO:128；SEQ IDNO:130；SEQ ID NO:132；SEQ ID NO:134；SEQ ID NO:136；SEQ ID NO:138；SEQ ID NO:140；或SEQ ID NO:142。在其它实施方案中，所述基因与以下各项基本上相同：SEQ ID NO:20；SEQ ID NO:22；SEQ ID NO:24；SEQ ID NO:26；SEQ ID NO:28；SEQ ID NO:30；SEQ ID NO:52；SEQ ID NO:96；SEQ ID NO:100；SEQ ID NO:128；SEQ ID NO:130；SEQ ID NO:132；SEQID NO:134；SEQ ID NO:136；SEQ ID NO:138；SEQ ID NO:140；或SEQ ID NO:142，并且所述核苷酸序列编码保留由以下各项编码的蛋白质的2型二酰基甘油酰基转移酶活性的蛋白质，但是在核苷酸序列上，例如由于天然等位基因变异或诱变而不同：SEQ ID NO:19；SEQID NO:21；SEQ ID NO:23；SEQ ID NO:25；SEQ ID NO:27；SEQ ID NO:29；SEQ ID NO:51；SEQID NO:95；SEQ ID NO:99；SEQ ID NO:127；SEQ ID NO:129；SEQ ID NO:131；SEQ ID NO:133；SEQ ID NO:135；SEQ ID NO:137；SEQ ID NO:139；或SEQ ID NO:141。
2型二酰基甘油酰基转移酶蛋白可以具有以下各项中所示的氨基酸序列：SEQ IDNO:19；SEQ ID NO:21；SEQ ID NO:23；SEQ ID NO:25；SEQ ID NO:27；SEQ ID NO:29；SEQ IDNO:51；SEQ ID NO:95；SEQ ID NO:99；SEQ ID NO:127；SEQ ID NO:129；SEQ ID NO:131；SEQID NO:133；SEQ ID NO:135；SEQ ID NO:137；SEQ ID NO:139；或SEQ ID NO:141。在其它实施方案中，2型二酰基甘油酰基转移酶蛋白与以下各项基本上相同：SEQ ID NO:19；SEQ IDNO:21；SEQ ID NO:23；SEQ ID NO:25；SEQ ID NO:27；SEQ ID NO:29；SEQ ID NO:51；SEQ IDNO:95；SEQ ID NO:99；SEQ ID NO:127；SEQ ID NO:129；SEQ ID NO:131；SEQ ID NO:133；SEQ ID NO:135；SEQ ID NO:137；SEQ ID NO:139；或SEQ ID NO:141，并且保留以下各项的蛋白质的功能活性，但是在氨基酸序列上，例如由于天然等位基因变异或诱变而不同：SEQID NO:19；SEQ ID NO:21；SEQ ID NO:23；SEQ ID NO:25；SEQ ID NO:27；SEQ ID NO:29；SEQID NO:51；SEQ ID NO:95；SEQ ID NO:99；SEQ ID NO:127；SEQ ID NO:129；SEQ ID NO:131；SEQ ID NO:133；SEQ ID NO:135；SEQ ID NO:137；SEQ ID NO:139；或SEQ ID NO:141。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、延长酶、二酰基甘油酰基转移酶、甘油-3-磷酸酰基转移酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或磷脂:二酰基甘油酰基转移酶。举例来说，所述转化的细胞可以包含提高DGA1蛋白的活性的遗传修饰和提高DGA2蛋白的活性的遗传修饰。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸酰基转移酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。举例来说，所述转化的细胞可以包含(1)由用编码外源DGA1蛋白的核酸转化组成的遗传修饰；以及(2)天然DGAT2基因中的敲除突变。类似地，所述转化的细胞可以包含(1)由用编码外源DGA1蛋白的核酸转化组成的遗传修饰；以及(2)降低天然Δ12去饱和酶蛋白的活性的遗传修饰。
c.提高3型二酰基甘油酰基转移酶的活性
在一些实施方案中，所述酰基转移酶蛋白是3型二酰基甘油酰基转移酶蛋白。所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用提高所述细胞中3型二酰基甘油酰基转移酶蛋白的活性的核酸转化。
所述核酸可以编码3型二酰基甘油酰基转移酶基因。在一些实施方案中，所述基因是DGAT3。在一些实施方案中，所述基因来自蓖麻(Ricinus communis)或花生(Arachishypogaea)。
在一些实施方案中，所述核酸包含核苷酸序列，所述核苷酸序列与SEQ ID NO:152或SEQ ID NO:154中所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性。所述核酸可以包含SEQ ID NO:152或SEQ IDNO:154中所示的核苷酸序列。
在一些实施方案中，所述核酸编码氨基酸序列，所述氨基酸序列与以下各项中所示的序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:151；或SEQ ID NO:153、或其生物活性部分。所述核酸可以编码SEQ ID NO:151或SEQ ID NO:153中所示的氨基酸序列。
包含编码3型二酰基甘油酰基转移酶蛋白的基因的核酸可以包含SEQ ID NO:152或SEQ ID NO:154中所示的核苷酸序列。在其它实施方案中，所述基因与以下各项基本上相同：SEQ ID NO:4；SEQ ID NO:8；SEQ ID SEQ ID NO:152；或SEQ ID NO:154，并且所述核苷酸序列编码保留由SEQ ID NO:151或SEQ ID NO:153编码的蛋白质的3型二酰基甘油酰基转移酶活性的蛋白质，但是在核苷酸序列上，例如由于天然等位基因变异或诱变而不同。
3型二酰基甘油酰基转移酶蛋白可以具有SEQ ID NO:151或SEQ ID NO:153中所示的氨基酸序列。在其它实施方案中，3型二酰基甘油酰基转移酶蛋白与SEQ ID NO:151或SEQID NO:153基本上相同，并且保留SEQ ID NO:151或SEQ ID NO:153的蛋白质的功能活性，但是在氨基酸序列上，例如由于天然等位基因变异或诱变而不同。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、延长酶、二酰基甘油酰基转移酶、甘油-3-磷酸酰基转移酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或磷脂:二酰基甘油酰基转移酶。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸酰基转移酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。
d.提高甘油-3-磷酸酰基转移酶的活性
在一些实施方案中，所述酰基转移酶蛋白是甘油-3-磷酸酰基转移酶蛋白。所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用提高所述细胞中甘油-3-磷酸酰基转移酶蛋白的活性的核酸转化。
所述核酸可以编码甘油-3-磷酸酰基转移酶基因。在一些实施方案中，所述基因是SCT1。在一些实施方案中，所述基因来自噬腺嘌呤阿克苏拉酵母、三角褐指藻、圆红冬孢酵母、粘红酵母、禾本红酵母、酿酒酵母、或解脂耶氏酵母。所述基因可以来自噬腺嘌呤阿克苏拉酵母或酿酒酵母。
在一些实施方案中，所述核酸包含核苷酸序列，所述核苷酸序列与以下各项中所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:18；SEQ ID NO:42；SEQ ID NO:44；SEQ ID NO:46；SEQ IDNO:48；SEQ ID NO:116；或SEQ ID NO:118。所述核酸可以包含以下各项中所示的核苷酸序列：SEQ ID NO:18；SEQ ID NO:42；SEQ ID NO:44；SEQ ID NO:46；SEQ ID NO:48；SEQ IDNO:116；或SEQ ID NO:118。在一些实施方案中，所述核酸包含核苷酸序列，所述核苷酸序列与SEQ ID NO:42或SEQ ID NO:44中所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性。所述核酸可以包含SEQ ID NO:42或SEQ ID NO:44中所示的核苷酸序列。
在一些实施方案中，所述核酸编码氨基酸序列，所述氨基酸序列与以下各项中所示的序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:17；SEQ ID NO:41；SEQID NO:43；SEQ ID NO:45；SEQ ID NO:47；SEQ ID NO:115；或SEQ ID NO:117、或其生物活性部分。所述核酸可以编码以下各项中所示的氨基酸序列：SEQ ID NO:17；SEQ ID NO:41；SEQID NO:43；SEQ ID NO:45；SEQ ID NO:47；SEQ ID NO:115；或SEQ ID NO:117。在一些实施方案中，所述核酸编码氨基酸序列，所述氨基酸序列与以下各项中所示的序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:41；或SEQ ID NO:43、或其生物活性部分。所述核酸可以编码SEQ ID NO:41或SEQ ID NO:43中所示的氨基酸序列。
包含编码甘油-3-磷酸酰基转移酶蛋白的基因的核酸可以包含以下各项中所示的核苷酸序列：SEQ ID NO:18；SEQ ID NO:42；SEQ ID NO:44；SEQ ID NO:46；SEQ ID NO:48；SEQ ID NO:116；或SEQ ID NO:118。在其它实施方案中，所述基因与以下各项基本上相同：SEQ ID NO:18；SEQ ID NO:42；SEQ ID NO:44；SEQ ID NO:46；SEQ ID NO:48；SEQ ID NO:116；或SEQ ID NO:118，并且所述核苷酸序列编码保留由以下各项编码的蛋白质的甘油-3-磷酸酰基转移酶活性的蛋白质，但是在核苷酸序列上，例如由于天然等位基因变异或诱变而不同：SEQ ID NO:17；SEQ ID NO:41；SEQ ID NO:43；SEQ ID NO:45；SEQ ID NO:47；SEQID NO:115；或SEQ ID NO:117。
甘油-3-磷酸酰基转移酶蛋白可以具有以下各项中所示的氨基酸序列：SEQ IDNO:17；SEQ ID NO:41；SEQ ID NO:43；SEQ ID NO:45；SEQ ID NO:47；SEQ ID NO:115；或SEQID NO:117。在其它实施方案中，甘油-3-磷酸酰基转移酶蛋白与以下各项基本上相同：SEQID NO:17；SEQ ID NO:41；SEQ ID NO:43；SEQ ID NO:45；SEQ ID NO:47；SEQ ID NO:115；或SEQ ID NO:117，并且保留以下各项的蛋白质的功能活性，但是在氨基酸序列上，例如由于天然等位基因变异或诱变而不同：SEQ ID NO:17；SEQ ID NO:41；SEQ ID NO:43；SEQ IDNO:45；SEQ ID NO:47；SEQ ID NO:115；或SEQ ID NO:117。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、延长酶、二酰基甘油酰基转移酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或磷脂:二酰基甘油酰基转移酶。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸酰基转移酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。举例来说，所述转化的细胞可以包含(1)由用编码外源甘油-3-磷酸酰基转移酶蛋白的核酸转化组成的遗传修饰；以及(2)天然SCT1基因中的敲除突变。类似地，所述转化的细胞可以包含(1)增加甘油-3-磷酸酰基转移酶蛋白的表达的遗传修饰；以及(2)降低天然Δ12去饱和酶蛋白的活性的遗传修饰。
e.提高磷脂:二酰基甘油酰基转移酶的活性
在一些实施方案中，所述酰基转移酶蛋白是磷脂:二酰基甘油酰基转移酶蛋白。所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用提高所述细胞中磷脂:二酰基甘油酰基转移酶蛋白的活性的核酸转化。
所述核酸可以编码磷脂:二酰基甘油酰基转移酶基因。在一些实施方案中，所述基因是LRO1。在一些实施方案中，所述基因来自噬腺嘌呤阿克苏拉酵母或解脂耶氏酵母。
在一些实施方案中，所述核酸包含核苷酸序列，所述核苷酸序列与SEQ ID NO:106或SEQ ID NO:110中所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性。所述核酸可以包含SEQ ID NO:106或SEQ IDNO:110中所示的核苷酸序列。
在一些实施方案中，所述核酸编码氨基酸序列，所述氨基酸序列与以下各项中所示的序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:105；或SEQ ID NO:109、或其生物活性部分。所述核酸可以编码SEQ ID NO:105或SEQ ID NO:109中所示的氨基酸序列。
包含编码磷脂:二酰基甘油酰基转移酶蛋白的基因的核酸可以包含SEQ ID NO:106或SEQ ID NO:110中所示的核苷酸序列。在其它实施方案中，所述基因与SEQ ID NO:106或SEQ ID NO:110基本上相同，并且所述核苷酸序列编码保留由SEQ ID NO:105或SEQ IDNO:109编码的蛋白质的磷脂:二酰基甘油酰基转移酶活性的蛋白质，但是在核苷酸序列上，例如由于天然等位基因变异或诱变而不同。
磷脂:二酰基甘油酰基转移酶蛋白可以具有SEQ ID NO:105或SEQ ID NO:109中所示的氨基酸序列。在其它实施方案中，磷脂:二酰基甘油酰基转移酶蛋白与SEQ ID NO:105或SEQ ID NO:109基本上相同，并且保留SEQ ID NO:105或SEQ ID NO:109的蛋白质的功能活性，但是在氨基酸序列上，例如由于天然等位基因变异或诱变而不同。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、延长酶、二酰基甘油酰基转移酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或甘油-3-磷酸酰基转移酶。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸酰基转移酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。
f.提高甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶的活性
在一些实施方案中，所述酰基转移酶蛋白是甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白。所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用提高所述细胞中甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白的活性的核酸转化。
所述核酸可以编码甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶基因。在一些实施方案中，所述基因是GPT2。在一些实施方案中，所述基因来自酿酒酵母、达氏诺莫维酵母(Naumovozyma dairenensis)、德尔布有孢圆酵母(Torulaspora delbrueckii)或卡氏诺莫维酵母(Naumovozyma castellii)。
在一些实施方案中，所述核酸包含核苷酸序列，所述核苷酸序列与以下各项中所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:120；SEQ ID NO:122；SEQ ID NO:124；或SEQ ID NO:126。所述核酸可以包含以下各项中所示的核苷酸序列：SEQ ID NO:120；SEQ ID NO:122；SEQ IDNO:124；或SEQ ID NO:126。
在一些实施方案中，所述核酸编码氨基酸序列，所述氨基酸序列与以下各项中所示的序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:119；SEQ ID NO:121；SEQID NO:123；或SEQ ID NO:125、或其生物活性部分。所述核酸可以编码以下各项中所示的氨基酸序列：SEQ ID NO:119；SEQ ID NO:121；SEQ ID NO:123；或SEQ ID NO:125。
包含编码甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白的基因的核酸可以包含以下各项中所示的核苷酸序列：SEQ ID NO:120；SEQ ID NO:122；SEQ ID NO:124；或SEQID NO:126。在其它实施方案中，所述基因与以下各项基本上相同：SEQ ID NO:120；SEQ IDNO:122；SEQ ID NO:124；或SEQ ID NO:126，并且所述核苷酸序列编码保留由以下各项编码的蛋白质的甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶活性的蛋白质，但是在核苷酸序列上，例如由于天然等位基因变异或诱变而不同：SEQ ID NO:119；SEQ ID NO:121；SEQ IDNO:123；或SEQ ID NO:125。
甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白可以具有以下各项中所示的氨基酸序列：SEQ ID NO:119；SEQ ID NO:121；SEQ ID NO:123；或SEQ ID NO:125。在其它实施方案中，甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白与以下各项基本上相同：SEQ IDNO:119；SEQ ID NO:121；SEQ ID NO:123；或SEQ ID NO:125，并且保留以下各项的蛋白质的功能活性，但是在氨基酸序列上，例如由于天然等位基因变异或诱变而不同：SEQ ID NO:119；SEQ ID NO:121；SEQ ID NO:123；或SEQ ID NO:125。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、延长酶、二酰基甘油酰基转移酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、磷脂:二酰基甘油酰基转移酶、和/或甘油-3-磷酸酰基转移酶。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸酰基转移酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。举例来说，所述转化的细胞可以包含(1)由用编码外源甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白的核酸转化组成的遗传修饰；以及(2)天然GPT2基因中的敲除突变。
g.提高sn-2酰基甘油脂肪酰基转移酶的活性
在一些实施方案中，所述酰基转移酶蛋白是sn-2酰基甘油脂肪酰基转移酶蛋白。所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用提高所述细胞中sn-2酰基甘油脂肪酰基转移酶蛋白的活性的核酸转化。
所述核酸可以编码sn-2酰基甘油脂肪酰基转移酶基因。在一些实施方案中，所述基因是SLC1或SLC4。在一些实施方案中，所述基因来自噬腺嘌呤阿克苏拉酵母、酿酒酵母、三角褐指藻、圆红冬孢酵母、小红酵母(Rhodotorula minuta)、禾本红酵母、或解脂耶氏酵母。
在一些实施方案中，所述核酸包含核苷酸序列，所述核苷酸序列与以下各项中所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:60；SEQ ID NO:62；SEQ ID NO:64；SEQ ID NO:66；SEQ IDNO:68；SEQ ID NO:70；SEQ ID NO:72；SEQ ID NO:74；SEQ ID NO:76；或SEQ ID NO:78。所述核酸可以包含以下各项中所示的核苷酸序列：SEQ ID NO:60；SEQ ID NO:62；SEQ ID NO:64；SEQ ID NO:66；SEQ ID NO:68；SEQ ID NO:70；SEQ ID NO:72；SEQ ID NO:74；SEQ IDNO:76；或SEQ ID NO:78。
在一些实施方案中，所述核酸编码氨基酸序列，所述氨基酸序列与以下各项中所示的序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:59；SEQ ID NO:61；SEQID NO:63；SEQ ID NO:65；SEQ ID NO:67；SEQ ID NO:69；SEQ ID NO:71；SEQ ID NO:73；SEQID NO:75；或SEQ ID NO:77、或其生物活性部分。所述核酸可以编码以下各项中所示的氨基酸序列：SEQ ID NO:59；SEQ ID NO:61；SEQ ID NO:63；SEQ ID NO:65；SEQ ID NO:67；SEQID NO:69；SEQ ID NO:71；SEQ ID NO:73；SEQ ID NO:75；或SEQ ID NO:77。
包含编码sn-2酰基甘油脂肪酰基转移酶蛋白的基因的核酸可以包含以下各项中所示的核苷酸序列：SEQ ID NO:60；SEQ ID NO:62；SEQ ID NO:64；SEQ ID NO:66；SEQ IDNO:68；SEQ ID NO:70；SEQ ID NO:72；SEQ ID NO:74；SEQ ID NO:76；或SEQ ID NO:78。在其它实施方案中，所述基因与以下各项基本上相同：SEQ ID NO:60；SEQ ID NO:62；SEQ IDNO:64；SEQ ID NO:66；SEQ ID NO:68；SEQ ID NO:70；SEQ ID NO:72；SEQ ID NO:74；SEQ IDNO:76；或SEQ ID NO:78，并且所述核苷酸序列编码保留由以下各项编码的蛋白质的sn-2酰基甘油脂肪酰基转移酶活性的蛋白质，但是在核苷酸序列上，例如由于天然等位基因变异或诱变而不同：SEQ ID NO:59；SEQ ID NO:61；SEQ ID NO:63；SEQ ID NO:65；SEQ ID NO:67；SEQ ID NO:69；SEQ ID NO:71；SEQ ID NO:73；SEQ ID NO:75；或SEQ ID NO:77。
sn-2酰基甘油脂肪酰基转移酶蛋白可以具有以下各项中所示的氨基酸序列：SEQID NO:59；SEQ ID NO:61；SEQ ID NO:63；SEQ ID NO:65；SEQ ID NO:67；SEQ ID NO:69；SEQID NO:71；SEQ ID NO:73；SEQ ID NO:75；或SEQ ID NO:77。在其它实施方案中，sn-2酰基甘油脂肪酰基转移酶蛋白与以下各项基本上相同：SEQ ID NO:59；SEQ ID NO:61；SEQ ID NO:63；SEQ ID NO:65；SEQ ID NO:67；SEQ ID NO:69；SEQ ID NO:71；SEQ ID NO:73；SEQ IDNO:75；或SEQ ID NO:77，并且保留以下各项的蛋白质的功能活性，但是在氨基酸序列上，例如由于天然等位基因变异或诱变而不同：SEQ ID NO:59；SEQ ID NO:61；SEQ ID NO:63；SEQID NO:65；SEQ ID NO:67；SEQ ID NO:69；SEQ ID NO:71；SEQ ID NO:73；SEQ ID NO:75；或SEQ ID NO:77。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、延长酶、二酰基甘油酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、磷脂:二酰基甘油酰基转移酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或甘油-3-磷酸酰基转移酶。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸酰基转移酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。
h.提高溶血磷脂酸酰基转移酶的活性
在一些实施方案中，所述酰基转移酶蛋白是溶血磷脂酸酰基转移酶蛋白。所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用提高所述细胞中溶血磷脂酸酰基转移酶蛋白的活性的核酸转化。
所述核酸可以编码溶血磷脂酸酰基转移酶基因。在一些实施方案中，所述基因是LOA1。在一些实施方案中，所述基因来自噬腺嘌呤阿克苏拉酵母、酿酒酵母、或解脂耶氏酵母。
在一些实施方案中，所述核酸包含核苷酸序列，所述核苷酸序列与以下各项中所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:80；SEQ ID NO:82；或SEQ ID NO:84。所述核酸可以包含以下各项中所示的核苷酸序列：SEQ ID NO:80；SEQ ID NO:82；或SEQ ID NO:84。
在一些实施方案中，所述核酸编码氨基酸序列，所述氨基酸序列与以下各项中所示的序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:79；SEQ ID NO:81；或SEQID NO:83、或其生物活性部分。所述核酸可以编码以下各项中所示的氨基酸序列：SEQ IDNO:79；SEQ ID NO:81；或SEQ ID NO:83。
包含编码溶血磷脂酸酰基转移酶蛋白的基因的核酸可以包含以下各项中所示的核苷酸序列：SEQ ID NO:80；SEQ ID NO:82；或SEQ ID NO:84。在其它实施方案中，所述基因与以下各项基本上相同：SEQ ID NO:80；SEQ ID NO:82；或SEQ ID NO:84，并且所述核苷酸序列编码保留由以下各项编码的蛋白质的溶血磷脂酸酰基转移酶活性的蛋白质，但是在核苷酸序列上，例如由于天然等位基因变异或诱变而不同：SEQ ID NO:79；SEQ ID NO:81；或SEQ ID NO:83。
溶血磷脂酸酰基转移酶蛋白可以具有以下各项中所示的氨基酸序列：SEQ ID NO:79；SEQ ID NO:81；或SEQ ID NO:83。在其它实施方案中，溶血磷脂酸酰基转移酶蛋白与以下各项基本上相同：SEQ ID NO:79；SEQ ID NO:81；或SEQ ID NO:83，并且保留以下各项的蛋白质的功能活性，但是在氨基酸序列上，例如由于天然等位基因变异或诱变而不同：SEQID NO:79；SEQ ID NO:81；或SEQ ID NO:83。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、延长酶、二酰基甘油酰基转移酶、sn-2酰基甘油脂肪酰基转移酶、磷脂酸磷酸酶、磷脂:二酰基甘油酰基转移酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或甘油-3-磷酸酰基转移酶。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸酰基转移酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。
4.提高磷脂酸磷酸酶的活性
在一些方面，本发明涉及一种转化的细胞，所述细胞包含遗传修饰，其中所述遗传修饰提高所述细胞中磷脂酸磷酸酶蛋白的活性。所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用提高所述细胞中磷脂酸磷酸酶蛋白的活性的核酸转化。
所述核酸可以编码磷脂酸磷酸酶基因。在一些实施方案中，所述基因是PAH1。在一些实施方案中，所述基因来自噬腺嘌呤阿克苏拉酵母、酿酒酵母、或解脂耶氏酵母。
在一些实施方案中，所述核酸包含核苷酸序列，所述核苷酸序列与以下各项中所示的序列具有至少70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:54；SEQ ID NO:56；或SEQ ID NO:58。所述核酸可以包含以下各项中所示的核苷酸序列：SEQ ID NO:54；SEQ ID NO:56；或SEQ ID NO:58。
在一些实施方案中，所述核酸编码氨基酸序列，所述氨基酸序列与以下各项中所示的序列具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％或更大的序列同源性：SEQ ID NO:53；SEQ ID NO:55；或SEQID NO:57、或其生物活性部分。所述核酸可以编码以下各项中所示的氨基酸序列：SEQ IDNO:53；SEQ ID NO:55；或SEQ ID NO:57。
包含编码磷脂酸磷酸酶蛋白的基因的核酸可以包含以下各项中所示的核苷酸序列：SEQ ID NO:54；SEQ ID NO:56；或SEQ ID NO:58。在其它实施方案中，所述基因与以下各项基本上相同：SEQ ID NO:54；SEQ ID NO:56；或SEQ ID NO:58，并且所述核苷酸序列编码保留由以下各项编码的蛋白质的磷脂酸磷酸酶活性的蛋白质，但是在核苷酸序列上，例如由于天然等位基因变异或诱变而不同：SEQ ID NO:53；SEQ ID NO:55；或SEQ ID NO:57。
磷脂酸磷酸酶蛋白可以具有以下各项中所示的氨基酸序列：SEQ ID NO:53；SEQID NO:55；或SEQ ID NO:57。在其它实施方案中，磷脂酸磷酸酶蛋白与以下各项基本上相同：SEQ ID NO:53；SEQ ID NO:55；或SEQ ID NO:57，并且保留以下各项的蛋白质的功能活性，但是在氨基酸序列上，例如由于天然等位基因变异或诱变而不同：SEQ ID NO:53；SEQID NO:55；或SEQ ID NO:57。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、延长酶、二酰基甘油酰基转移酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂:二酰基甘油酰基转移酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或甘油-3-磷酸酰基转移酶。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸酰基转移酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。
C.用于降低天然蛋白质的活性的核酸和方法
在一些实施方案中，所述转化的含油细胞包含降低天然蛋白质的活性的遗传修饰。这样的遗传修饰可以影响调节天然蛋白质转录的蛋白质，包括减少转录激活子的表达和/或增加转录阻遏子的表达的修饰。影响调节蛋白的修饰可以减少天然蛋白质的表达并且改变使细胞平衡朝向油酸积聚增加偏移的其它基因表达谱。或者，所述遗传修饰可以是引入干扰核酸，如小干扰RNA、或编码干扰核酸的核酸。在其它实施方案中，所述遗传修饰由核酸和编码天然蛋白质的基因的调节区的同源重组组成。基因的调节区可以包括操纵子、启动子、启动子上游的序列、增强子、和/或基因下游的序列。
在一些实施方案中，所述转化的含油细胞包含由同源重组事件组成的遗传修饰。在某些实施方案中，所述转化的细胞包含由天然基因与核酸之间的同源重组事件组成的遗传修饰。因此，所述遗传修饰使天然基因缺失、防止它转录、或防止可以被翻译成完全活性蛋白质的基因转录。同源重组事件可以使天然基因的一部分突变或缺失。举例来说，同源重组事件可以使天然酶的活性位点中的一个或多个残基突变，从而降低酶的效率或使它变得无活性。或者，同源重组事件可以影响翻译后修饰、折叠、稳定性、或细胞内的定位。在一些实施方案中，同源重组事件将启动子置换为驱动更少的转录的启动子。在其它实施方案中，同源重组事件使启动子突变以损害它驱动转录的能力。在某些实施方案中，所述遗传修饰是敲除突变。
敲除突变可以使一个或多个基因缺失。此外，敲除突变可以将天然基因用编码不同蛋白质的外源基因取代。所述外源基因可以与外源启动子可操作地连接。在某些实施方案中，所述基因不与外源启动子连接，并且相反，所述基因被配置成与天然基因重组以使得天然基因的启动子驱动所述外源基因的转录。因此，如果所述基因随机整合到细胞的基因组中，那么它不太可能被表达。用于形成敲除的方法是本领域公知的(参见例如Fickers等,J.Microbiological Methods 55:727(2003))。
在某些实施方案中，所述遗传修饰包含两个同源重组事件。在第一个事件中，编码基因的一部分的核酸与天然基因重组，并且在第二个事件中，编码所述基因的其余部分的核酸与天然基因重组。所述基因的这两个部分被设计成使得除非这两个部分彼此重组，否则它们都不是功能性的。这两个事件进一步降低了在随机整合事件后所述基因可能被表达的可能性。
在某些实施方案中，所述基因编码标记蛋白，如显性选择性标记。因此，可以通过针对所述标记进行筛选来选择敲除细胞。在一些实施方案中，所述显性选择性标记是药物抗性标记。药物抗性标记是在由细胞表达时，允许细胞在原本通常将抑制细胞生长和/或存活的药物存在下生长和/或存活的显性选择性标记。可以通过在所述药物存在下培养细胞来选择表达药物抗性标记的细胞。在一些实施方案中，所述药物抗性标记是抗生素抗性标记。在一些实施方案中，所述药物抗性标记赋予对药物的抗性，所述药物选自由以下各项组成的组：两性霉素B(Amphotericin B)、杀假丝菌素(Candicidin)、菲律宾菌素(Filipin)、哈霉素(Hamycin)、纳他霉素(Natamycin)、制霉菌素(Nystatin)、龟裂杀菌素(Rimocidin)、联苯苄唑(Bifonazole)、布康唑(Butoconazole)、克霉唑(Clotrimazole)、益康唑(Econazole)、芬替康唑(Fenticonazole)、异康唑(Isoconazole)、酮康唑(Ketoconazole)、卢立康唑(Luliconazole)、咪康唑(Miconazole)、奥莫康唑(Omoconazole)、奥昔康唑(Oxiconazole)、舍他康唑(Sertaconazole)、硫康唑(Sulconazole)、噻康唑(Tioconazole)、阿巴康唑(Albaconazole)、氟康唑(Fluconazole)、艾沙康唑(Isavuconazole)、伊曲康唑(Itraconazole)、泊沙康唑(Posaconazole)、雷夫康唑(Ravuconazole)、特康唑(Terconazole)、伏立康唑(Voriconazole)、阿巴芬净(Abafungin)、阿莫罗芬(Amorolfin)、布替萘芬(Butenafine)、萘替芬(Naftifine)、特比萘芬(Terbinafine)、阿尼芬净(Anidulafungin)、卡泊芬净(Caspofungin)、米卡芬净(Micafungin)、苯甲酸、环吡酮(Ciclopirox)、氟胞嘧啶、5-氟胞嘧啶、灰黄霉素(Griseofulvin)、卤普罗近(Haloprogin)、水蓼二醛(Polygodial)、托萘酯(Tolnaftate)、结晶紫、阿米卡星(Amikacin)、庆大霉素(Gentamicin)、卡那霉素(Kanamycin)、新霉素(Neomycin)、奈替米星(Netilmicin)、妥布霉素(Tobramycin)、巴龙霉素(Paromomycin)、大观霉素(Spectinomycin)、格尔德霉素(Geldanamycin)、除莠霉素(Herbimycin)、利福昔明(Rifaximin)、链霉素(Streptomycin)、氯碳头孢(Loracarbef)、厄他培南(Ertapenem)、多利培南(Doripenem)、亚胺培南(Imipenem)、美罗培南(Meropenem)、头孢羟氨苄(Cefadroxil)、头孢唑啉(Cefazolin)、头孢噻吩(Cefalotin)、头孢氨苄(Cefalexin)、头孢克洛(Cefaclor)、头孢孟多(Cefamandole)、头孢西丁(Cefoxitin)、头孢丙烯(Cefprozil)、头孢呋辛(Cefuroxime)、头孢克肟(Cefixime)、头孢地尼(Cefdinir)、头孢妥仑(Cefditoren)、头孢哌酮(Cefoperazone)、头孢噻肟(Cefotaxime)、头孢泊肟(Cefpodoxime)、头孢他啶(Ceftazidime)、头孢布烯(Ceftibuten)、头孢唑肟(Ceftizoxime)、头孢曲松(Ceftriaxone)、头孢吡肟(Cefepime)、头孢洛林酯(Ceftarolinefosamil)、头孢比普(Ceftobiprole)、替考拉宁(Teicoplanin)、万古霉素(Vancomycin)、特拉万星(Telavancin)、克林霉素(Clindamycin)、林可霉素(Lincomycin)、达托霉素(Daptomycin)、阿奇霉素(Azithromycin)、克拉霉素(Clarithromycin)、地红霉素(Dirithromycin)、红霉素(Erythromycin)、罗红霉素(Roxithromycin)、醋竹桃霉素(Troleandomycin)、泰利霉素(Telithromycin)、螺旋霉素(Spiramycin)、氨曲南(Aztreonam)、呋喃唑酮(Furazolidone)、呋喃妥因(Nitrofurantoin)、利奈唑胺(Linezolid)、泊西唑来(Posizolid)、雷得唑来(Radezolid)、泰地唑来(Torezolid)、阿莫西林(Amoxicillin)、氨苄西林、阿洛西林(Azlocillin)、羧苄西林(Carbenicillin)、氯唑西林(Cloxacillin)、双氯西林(Dicloxacillin)、氟氯西林(Flucloxacillin)、美洛西林(Mezlocillin)、甲氧西林(Methicillin)、萘夫西林(Nafcillin)、苯唑西林(Oxacillin)、青霉素G、青霉素V、哌拉西林(Piperacillin)、青霉素G、替莫西林(Temocillin)、替卡西林(Ticarcillin)、克拉维酸(clavulanate)、舒巴坦(sulbactam)、他唑巴坦(tazobactam)、克拉维酸、杆菌肽(Bacitracin)、粘菌素(Colistin)、多粘菌素B(Polymyxin B)、环丙沙星(Ciprofloxacin)、依诺沙星(Enoxacin)、加替沙星(Gatifloxacin)、左氧氟沙星(Levofloxacin)、洛美沙星(Lomefloxacin)、莫西沙星(Moxifloxacin)、萘啶酸(Nalidixicacid)、诺氟沙星(Norfloxacin)、氧氟沙星(Ofloxacin)、曲伐沙星(Trovafloxacin)、格帕沙星(Grepafloxacin)、司帕沙星(Sparfloxacin)、替马沙星(Temafloxacin)、磺胺米隆(Mafenide)、磺胺醋酰(Sulfacetamide)、磺胺嘧啶(Sulfadiazine)、磺胺嘧啶银(Silversulfadiazine)、磺胺二甲氧嘧啶(Sulfadimethoxine)、磺胺甲二唑(Sulfamethizole)、磺胺甲唑(Sulfamethoxazole)、对氨基苯磺酰胺(Sulfanilimide)、柳氮磺胺吡啶(Sulfasalazine)、磺胺异唑(Sulfisoxazole)、甲氧苄啶-磺胺甲唑(Trimethoprim-Sulfamethoxazole)、复方新诺明(Co-trimoxazole)、亚磺酰胺基二氨基偶氮苯(Sulfonamidochrysoidine)、地美环素(Demeclocycline)、强力霉素(Doxycycline)、米诺环素(Minocycline)、土霉素(Oxytetracycline)、四环素(Tetracycline)、氯法齐明(Clofazimine)、氨苯砜(Dapsone)、卷曲霉素(Capreomycin)、环丝氨酸(Cycloserine)、乙胺丁醇(Ethambutol)、乙硫异烟胺(Ethionamide)、异烟肼(Isoniazid)、吡嗪酰胺(Pyrazinamide)、利福平(Rifampicin)、利福布丁(Rifabutin)、利福喷丁(Rifapentine)、链霉素、胂凡纳明(Arsphenamine)、氯霉素(Chloramphenicol)、磷霉素(Fosfomycin)、夫西地酸(Fusidic acid)、甲硝唑(Metronidazole)、莫匹罗星(Mupirocin)、平板霉素(Platensimycin)、奎奴普丁(Quinupristin)、达福普汀(Dalfopristin)、甲砜霉素(Thiamphenicol)、替加环素(Tigecycline)、替硝唑(Tinidazole)、甲氧苄啶(Trimethoprim)、遗传霉素(Geneticin)、诺尔丝菌素、潮霉素(Hygromycin)、博来霉素(Bleomycin)、以及嘌呤霉素(Puromycin)。
在一些实施方案中，所述显性选择性标记是营养标记。营养标记是当由细胞表达时使细胞能够使用一种或多种特定的营养源生长或存活的显性选择性标记。可以通过在限制营养条件下培养细胞来选择表达营养标记的细胞，其中表达营养标记的细胞可以存活和/或生长，但是缺少营养标记的细胞则不能。在一些实施方案中，营养标记选自由以下各项组成的组：乳清酸核苷5-磷酸脱羧酶、亚磷酸特异性氧化还原酶、α-酮戊二酸依赖性次磷酸双加氧酶、碱性磷酸酶、氰胺水合酶、三聚氰胺脱氨酶、氰尿酸酰胺水解酶、缩二脲水解酶、尿素酰胺水解酶、氰尿酰胺氨基水解酶、鸟嘌呤脱氨酶、磷酸二酯酶、磷酸三酯酶、亚磷酸氢化酶、甘油磷酸二酯酶、对硫磷水解酶、亚磷酸脱氢酶、二苯并噻吩脱硫酶、芳族脱亚磺酸酶、NADH依赖性FMN还原酶、氨基嘌呤转运蛋白、羟胺氧化还原酶、转化酶、β-葡萄糖苷酶、α-葡萄糖苷酶、β-半乳糖苷酶、α-半乳糖苷酶、淀粉酶、纤维素酶、以及支链淀粉酶(Pullulonase)。
可以使用不同的方法来敲除酵母细胞中的基因(参见例如Dulermo等,BiochimicaBiophysica Acta 1831:1486(2013))。可以使用本文所公开的方法和本领域已知的其它方法来敲除其它物种，如噬腺嘌呤阿克苏拉酵母中的不同基因。
在一些实施方案中，遗传修饰使天然基因的表达减少了0.1％、0.2％、0.3％、0.4％、0.5％、0.6％、0.7％、0.8％、0.9％、1％、2％、3％、4％、5％、6％、7％、8％、9％、10％、25％、30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％、或100％。
在一些实施方案中，遗传修饰使天然蛋白质的效率降低了0.1％、0.2％、0.3％、0.4％、0.5％、0.6％、0.7％、0.8％、0.9％、1％、2％、3％、4％、5％、6％、7％、8％、9％、10％、25％、30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％、或100％。
在一些实施方案中，遗传修饰使天然蛋白质的活性降低了0.1％、0.2％、0.3％、0.4％、0.5％、0.6％、0.7％、0.8％、0.9％、1％、2％、3％、4％、5％、6％、7％、8％、9％、10％、25％、30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％、或100％。
1.降低天然Δ12去饱和酶的活性
在一些方面，本发明涉及一种转化的细胞，所述细胞包含遗传修饰，其中所述遗传修饰降低天然Δ12去饱和酶蛋白的活性。在一些实施方案中，所述遗传修饰是敲除突变。
所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用降低所述细胞中天然Δ12去饱和酶蛋白的活性的核酸转化。所述核酸可能能够与天然Δ12去饱和酶基因和/或天然Δ12去饱和酶基因的调节区中的核苷酸序列重组。在一些实施方案中，天然Δ12去饱和酶蛋白是由Δ12基因编码的。
在某些实施方案中，所述细胞是解脂耶氏酵母并且天然Δ12去饱和酶蛋白具有SEQ ID NO:1中所示的氨基酸序列。在一些实施方案中，所述细胞是解脂耶氏酵母，并且天然Δ12去饱和酶蛋白是由SEQ ID NO:2中所示的核苷酸序列编码的。
在某些实施方案中，所述细胞是噬腺嘌呤阿克苏拉酵母并且天然Δ12去饱和酶蛋白具有SEQ ID NO:49中所示的氨基酸序列。在一些实施方案中，所述细胞是噬腺嘌呤阿克苏拉酵母，并且天然Δ12去饱和酶蛋白是由SEQ ID NO:50中所示的核苷酸序列编码的。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、延长酶、二酰基甘油酰基转移酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、磷脂:二酰基甘油酰基转移酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或甘油-3-磷酸酰基转移酶。举例来说，所述转化的细胞可以包含(1)降低天然Δ12去饱和酶蛋白的活性的遗传修饰；以及(2)提高Δ9去饱和酶蛋白的活性的遗传修饰。类似地，所述转化的细胞可以包含(1)降低天然Δ12去饱和酶蛋白的活性的遗传修饰；以及(2)提高二酰基甘油酰基转移酶蛋白的活性的遗传修饰。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸酰基转移酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。
2.降低天然Δ9去饱和酶的活性
在一些方面，本发明涉及一种转化的细胞，所述细胞包含遗传修饰，其中所述遗传修饰降低天然Δ9去饱和酶蛋白的活性。在一些实施方案中，所述遗传修饰是敲除突变。
所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用降低所述细胞中天然Δ9去饱和酶蛋白的活性的核酸转化。所述核酸可能能够与天然Δ9去饱和酶基因和/或天然Δ9去饱和酶基因的调节区中的核苷酸序列重组。在一些实施方案中，天然Δ9去饱和酶蛋白是由Δ9、OLE1、或FAD1基因编码的。
在某些实施方案中，所述细胞是解脂耶氏酵母并且天然Δ9去饱和酶蛋白具有SEQID NO:3中所示的氨基酸序列。在一些实施方案中，所述细胞是解脂耶氏酵母，并且天然Δ9去饱和酶蛋白是由SEQ ID NO:4中所示的核苷酸序列编码的。
在某些实施方案中，所述细胞是噬腺嘌呤阿克苏拉酵母并且天然Δ9去饱和酶蛋白具有SEQ ID NO:7中所示的氨基酸序列。在一些实施方案中，所述细胞是噬腺嘌呤阿克苏拉酵母，并且天然Δ9去饱和酶蛋白是由SEQ ID NO:8中所示的核苷酸序列编码的。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶(例如外源Δ9去饱和酶)、延长酶、二酰基甘油酰基转移酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、磷脂:二酰基甘油酰基转移酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或甘油-3-磷酸酰基转移酶。举例来说，所述转化的细胞可以包含(1)降低天然Δ9去饱和酶蛋白的活性的遗传修饰；以及(2)由用编码Δ9去饱和酶蛋白，例如来自不同物种的Δ9去饱和酶蛋白的核酸转化组成的遗传修饰。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸酰基转移酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。
3.降低天然二酰基甘油酰基转移酶的活性
在一些方面，本发明涉及一种转化的细胞，所述细胞包含遗传修饰，其中所述遗传修饰降低天然二酰基甘油酰基转移酶蛋白的活性。在一些实施方案中，所述遗传修饰是敲除突变。
所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用降低所述细胞中天然二酰基甘油酰基转移酶蛋白的活性的核酸转化。所述核酸可能能够与天然二酰基甘油酰基转移酶基因和/或天然二酰基甘油酰基转移酶基因的调节区中的核苷酸序列重组。在一些实施方案中，天然二酰基甘油酰基转移酶蛋白是由DGAT1或DGAT2基因编码的。
在某些实施方案中，所述细胞是解脂耶氏酵母并且天然二酰基甘油酰基转移酶蛋白具有SEQ ID NO:19或SEQ ID NO:93中所示的氨基酸序列。在一些实施方案中，所述细胞是解脂耶氏酵母，并且天然二酰基甘油酰基转移酶蛋白是由SEQ ID NO:20或SEQ ID NO:94中所示的核苷酸序列编码的。
在某些实施方案中，所述细胞是噬腺嘌呤阿克苏拉酵母并且天然二酰基甘油酰基转移酶蛋白具有SEQ ID NO:51或SEQ ID NO:103中所示的氨基酸序列。在一些实施方案中，所述细胞是噬腺嘌呤阿克苏拉酵母，并且天然二酰基甘油酰基转移酶蛋白是由SEQ ID NO:52或SEQ ID NO:104中所示的核苷酸序列编码的。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、延长酶、二酰基甘油酰基转移酶(例如外源二酰基甘油酰基转移酶)、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、磷脂:二酰基甘油酰基转移酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或甘油-3-磷酸酰基转移酶。举例来说，所述转化的细胞可以包含(1)降低天然二酰基甘油酰基转移酶蛋白的活性的遗传修饰；以及(2)由用编码二酰基甘油酰基转移酶蛋白，例如来自不同物种的二酰基甘油酰基转移酶蛋白的核酸的转化组成的遗传修饰。类似地，所述转化的细胞可以包含(1)降低天然二酰基甘油酰基转移酶蛋白的活性的遗传修饰；以及(2)提高Δ9去饱和酶蛋白的活性的遗传修饰。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶(例如不同的二酰基甘油酰基转移酶)、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸酰基转移酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。举例来说，所述转化的细胞可以包含(1)降低天然DGA1蛋白的活性的遗传修饰；以及(2)降低天然DGA2蛋白的活性的遗传修饰。类似地，所述转化的细胞可以包含(1)降低天然二酰基甘油酰基转移酶蛋白的活性的遗传修饰；以及(2)降低天然Δ12去饱和酶蛋白的活性的遗传修饰。
4.降低天然三酰基甘油脂酶的活性
在一些方面，本发明涉及一种转化的细胞，所述细胞包含遗传修饰，其中所述遗传修饰降低天然三酰基甘油脂酶蛋白的活性。在一些实施方案中，所述遗传修饰是敲除突变。
所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用降低所述细胞中天然三酰基甘油脂酶蛋白的活性的核酸转化。所述核酸可能能够与天然三酰基甘油脂酶基因和/或天然三酰基甘油脂酶基因的调节区中的核苷酸序列重组。在一些实施方案中，天然三酰基甘油脂酶是由TGL3、TGL3/4、或TGL4基因编码的。
在某些实施方案中，所述细胞是解脂耶氏酵母并且天然三酰基甘油脂酶蛋白具有SEQ ID NO:91中所示的氨基酸序列。在一些实施方案中，所述细胞是解脂耶氏酵母，并且天然三酰基甘油脂酶蛋白是由SEQ ID NO:92中所示的核苷酸序列编码的。
在某些实施方案中，所述细胞是噬腺嘌呤阿克苏拉酵母并且天然三酰基甘油脂酶蛋白具有以下各项中所示的氨基酸序列：SEQ ID NO:85；SEQ ID NO:87；或SEQ ID NO:89。在一些实施方案中，所述细胞是噬腺嘌呤阿克苏拉酵母，并且天然三酰基甘油脂酶蛋白是由以下各项中所示的核苷酸序列编码的：SEQ ID NO:86；SEQ ID NO:88；或SEQ ID NO:90。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、延长酶、二酰基甘油酰基转移酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、磷脂:二酰基甘油酰基转移酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或甘油-3-磷酸酰基转移酶。举例来说，所述转化的细胞可以包含(1)降低天然三酰基甘油脂酶蛋白的活性的遗传修饰；以及(2)提高二酰基甘油酰基转移酶蛋白的活性的遗传修饰。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸酰基转移酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。
三酰基甘油脂酶通过去除一个或多个脂肪酸链来消耗细胞的三酰基甘油。因此，降低细胞的净三酰基甘油脂酶活性可以增加细胞的油酸。这一降低可以通过降低酶的效率，例如通过使它的活性位点中的氨基酸突变、或通过减少酶的表达来实现。举例来说，TGL3敲除突变将降低三酰基甘油脂酶的活性，这是因为它阻止细胞转录TGL3。三酰基甘油脂酶敲除描述于WO 2015/168531和USSN 61/987,098中(这两者均以引用的方式并入本文)。
在一些实施方案中，三酰基甘油脂酶是TGL3。在其它实施方案中，三酰基甘油脂酶是TGL3/4或TGL4。
解脂耶氏酵母中的TGL3基因编码三酰基甘油脂酶蛋白TGL3(SEQ ID NO:91)。SEQID NO:92含有TGL3核苷酸序列、100个上游核苷酸以及100个下游核苷酸。因此，可以使用SEQ ID NO:92核苷酸序列来设计能够与天然解脂耶氏酵母三酰基甘油脂酶基因中的核酸序列重组的核酸。
敲除盒SEQ ID NO:167和SEQ ID NO:168能够与解脂耶氏酵母中的天然TGL3基因重组。因此，在一些实施方案中，可以使用由SEQ ID NO:167和SEQ ID NO:168编码的核酸来在解脂耶氏酵母中产生三酰基甘油脂酶敲除突变。SEQ ID NO:167和SEQ ID NO:168各自含有潮霉素抗性基因hph的部分。这两个分离的序列都不编码功能性蛋白，但是这两个序列能够在成功重组时编码赋予潮霉素抗性的功能性激酶。此外，SEQ ID NO:167和SEQ ID NO:168都不含启动子或终止子，并且因此，它们依靠与解脂耶氏酵母TGL3基因同源重组来使hph基因转录和翻译。以这种方式，可以通过在含有潮霉素的培养基上培养细胞来选择成功转化的含油细胞。
敲除盒SEQ ID NO:167可以通过用引物NP1798(SEQ ID NO:165)和引物NP656(SEQID NO:164)扩增潮霉素抗性基因hph(SEQ ID NO:162)来制备。敲除盒SEQ ID NO:50可以通过用引物NP655(SEQ ID NO:163)和引物NP1799(SEQ ID NO:166)扩增潮霉素抗性基因hph(SEQ ID NO:162)来制备。
可以使用不同的方法来设计降低解脂耶氏酵母中TGL3的活性的核酸(BiochimicaBiophysica Acta 1831:1486-95(2013))。可以使用本文所公开的方法和本领域已知的其它方法来降低其它物种中三酰基甘油脂酶的活性。举例来说，可以使用这些方法来降低噬腺嘌呤阿克苏拉酵母的TGL3基因(SEQ ID NO:86)、噬腺嘌呤阿克苏拉酵母的TGL3/4基因(SEQ ID NO:88)、或噬腺嘌呤阿克苏拉酵母的TGL4基因(SEQ ID NO:90)的活性。类似地，这些方法一般适用于降低酵母和其它生物体中蛋白质的活性。
5.降低天然sn-2酰基甘油脂肪酰基转移酶的活性
在一些方面，本发明涉及一种转化的细胞，所述细胞包含遗传修饰，其中所述遗传修饰降低天然sn-2酰基甘油脂肪酰基转移酶蛋白的活性。在一些实施方案中，所述遗传修饰是敲除突变。
所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用降低所述细胞中天然sn-2酰基甘油脂肪酰基转移酶蛋白的活性的核酸转化。所述核酸可能能够与天然sn-2酰基甘油脂肪酰基转移酶基因和/或天然sn-2酰基甘油脂肪酰基转移酶基因的调节区中的核苷酸序列重组。在一些实施方案中，天然sn-2酰基甘油脂肪酰基转移酶蛋白是由SLC1或SLC4基因编码的。
在某些实施方案中，所述细胞是解脂耶氏酵母并且天然sn-2酰基甘油脂肪酰基转移酶蛋白具有SEQ ID NO:59或SEQ ID NO:65中所示的氨基酸序列。在一些实施方案中，所述细胞是解脂耶氏酵母，并且天然sn-2酰基甘油脂肪酰基转移酶蛋白是由SEQ ID NO:60或SEQ ID NO:66中所示的核苷酸序列编码的。
在某些实施方案中，所述细胞是噬腺嘌呤阿克苏拉酵母并且天然sn-2酰基甘油脂肪酰基转移酶蛋白具有SEQ ID NO:61或SEQ ID NO:63中所示的氨基酸序列。在一些实施方案中，所述细胞是噬腺嘌呤阿克苏拉酵母，并且天然sn-2酰基甘油脂肪酰基转移酶蛋白是由SEQ ID NO:62或SEQ ID NO:64中所示的核苷酸序列编码的。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、延长酶、二酰基甘油酰基转移酶、sn-2酰基甘油脂肪酰基转移酶(例如外源sn-2酰基甘油脂肪酰基转移酶)、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、磷脂:二酰基甘油酰基转移酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或甘油-3-磷酸酰基转移酶。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸酰基转移酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。
6.降低天然溶血磷脂酸酰基转移酶的活性
在一些方面，本发明涉及一种转化的细胞，所述细胞包含遗传修饰，其中所述遗传修饰降低天然溶血磷脂酸酰基转移酶蛋白的活性。在一些实施方案中，所述遗传修饰是敲除突变。
所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用降低所述细胞中天然溶血磷脂酸酰基转移酶蛋白的活性的核酸转化。所述核酸可能能够与天然溶血磷脂酸酰基转移酶基因和/或天然溶血磷脂酸酰基转移酶基因的调节区中的核苷酸序列重组。在一些实施方案中，天然溶血磷脂酸酰基转移酶蛋白是由LOA1基因编码的。
在某些实施方案中，所述细胞是解脂耶氏酵母并且天然溶血磷脂酸酰基转移酶蛋白具有SEQ ID NO:83中所示的氨基酸序列。在一些实施方案中，所述细胞是解脂耶氏酵母，并且天然溶血磷脂酸酰基转移酶蛋白是由SEQ ID NO:84中所示的核苷酸序列编码的。
在某些实施方案中，所述细胞是噬腺嘌呤阿克苏拉酵母并且天然溶血磷脂酸酰基转移酶蛋白具有SEQ ID NO:81中所示的氨基酸序列。在一些实施方案中，所述细胞是噬腺嘌呤阿克苏拉酵母，并且天然溶血磷脂酸酰基转移酶蛋白是由SEQ ID NO:82中所示的核苷酸序列编码的。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、延长酶、二酰基甘油酰基转移酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶(例如外源溶血磷脂酸酰基转移酶)、磷脂酸磷酸酶、磷脂:二酰基甘油酰基转移酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或甘油-3-磷酸酰基转移酶。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸酰基转移酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。
7.降低天然磷脂酸磷酸酶的活性
在一些方面，本发明涉及一种转化的细胞，所述细胞包含遗传修饰，其中所述遗传修饰降低天然磷脂酸磷酸酶蛋白的活性。在一些实施方案中，所述遗传修饰是敲除突变。
所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用降低所述细胞中天然磷脂酸磷酸酶蛋白的活性的核酸转化。所述核酸可能能够与天然磷脂酸磷酸酶基因和/或天然磷脂酸磷酸酶基因的调节区中的核苷酸序列重组。在一些实施方案中，天然磷脂酸磷酸酶蛋白是由PAH1基因编码的。
在某些实施方案中，所述细胞是解脂耶氏酵母并且天然磷脂酸磷酸酶蛋白具有SEQ ID NO:57中所示的氨基酸序列。在一些实施方案中，所述细胞是解脂耶氏酵母，并且天然磷脂酸磷酸酶蛋白是由SEQ ID NO:58中所示的核苷酸序列编码的。
在某些实施方案中，所述细胞是噬腺嘌呤阿克苏拉酵母并且天然磷脂酸磷酸酶蛋白具有SEQ ID NO:55中所示的氨基酸序列。在一些实施方案中，所述细胞是噬腺嘌呤阿克苏拉酵母，并且天然磷脂酸磷酸酶蛋白是由SEQ ID NO:56中所示的核苷酸序列编码的。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、延长酶、二酰基甘油酰基转移酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶(例如外源磷脂酸磷酸酶)、磷脂:二酰基甘油酰基转移酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或甘油-3-磷酸酰基转移酶。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然甘油-3-磷酸酰基转移酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。
8.降低天然甘油-3-磷酸酰基转移酶的活性
在一些方面，本发明涉及一种转化的细胞，所述细胞包含遗传修饰，其中所述遗传修饰降低天然甘油-3-磷酸酰基转移酶蛋白的活性。在一些实施方案中，所述遗传修饰是敲除突变。
所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用降低所述细胞中天然甘油-3-磷酸酰基转移酶蛋白的活性的核酸转化。所述核酸可能能够与天然甘油-3-磷酸酰基转移酶基因和/或天然甘油-3-磷酸酰基转移酶基因的调节区中的核苷酸序列重组。在一些实施方案中，天然甘油-3-磷酸酰基转移酶是由SCT1基因编码的。
在某些实施方案中，所述细胞是解脂耶氏酵母并且天然甘油-3-磷酸酰基转移酶蛋白具有SEQ ID NO:17中所示的氨基酸序列。在一些实施方案中，所述细胞是解脂耶氏酵母，并且天然甘油-3-磷酸酰基转移酶蛋白是由SEQ ID NO:18中所示的核苷酸序列编码的。
在某些实施方案中，所述细胞是噬腺嘌呤阿克苏拉酵母并且天然甘油-3-磷酸酰基转移酶蛋白具有SEQ ID NO:43中所示的氨基酸序列。在一些实施方案中，所述细胞是噬腺嘌呤阿克苏拉酵母，并且天然甘油-3-磷酸酰基转移酶蛋白是由SEQ ID NO:44中所示的核苷酸序列编码的。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、延长酶、二酰基甘油酰基转移酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、磷脂:二酰基甘油酰基转移酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或甘油-3-磷酸酰基转移酶(例如外源甘油-3-磷酸酰基转移酶)。举例来说，所述转化的细胞可以包含(1)降低天然甘油-3-磷酸酰基转移酶蛋白的活性的遗传修饰；以及(2)由用编码外源甘油-3-磷酸酰基转移酶蛋白的核酸转化组成的遗传修饰。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然磷脂:二酰基甘油酰基转移酶。举例来说，所述转化的细胞可以包含(1)降低天然甘油-3-磷酸酰基转移酶蛋白的活性的遗传修饰；以及(2)降低天然Δ12去饱和酶蛋白的活性的遗传修饰。
9.降低天然磷脂:二酰基甘油酰基转移酶的活性
在一些方面，本发明涉及一种转化的细胞，所述细胞包含遗传修饰，其中所述遗传修饰降低天然磷脂:二酰基甘油酰基转移酶蛋白的活性。在一些实施方案中，所述遗传修饰是敲除突变。
所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用降低所述细胞中天然磷脂:二酰基甘油酰基转移酶蛋白的活性的核酸转化。所述核酸可能能够与天然磷脂:二酰基甘油酰基转移酶基因和/或天然磷脂:二酰基甘油酰基转移酶基因的调节区中的核苷酸序列重组。在一些实施方案中，天然磷脂:二酰基甘油酰基转移酶蛋白是由LRO1基因编码的。
在某些实施方案中，所述细胞是解脂耶氏酵母并且天然磷脂:二酰基甘油酰基转移酶蛋白具有SEQ ID NO:109中所示的氨基酸序列。在一些实施方案中，所述细胞是解脂耶氏酵母，并且天然磷脂:二酰基甘油酰基转移酶蛋白是由SEQ ID NO:110中所示的核苷酸序列编码的。
在某些实施方案中，所述细胞是噬腺嘌呤阿克苏拉酵母并且天然磷脂:二酰基甘油酰基转移酶蛋白具有SEQ ID NO:105中所示的氨基酸序列。在一些实施方案中，所述细胞是噬腺嘌呤阿克苏拉酵母，并且天然磷脂:二酰基甘油酰基转移酶蛋白是由SEQ ID NO:106中所示的核苷酸序列编码的。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、延长酶、二酰基甘油酰基转移酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、磷脂:二酰基甘油酰基转移酶(例如外源磷脂:二酰基甘油酰基转移酶)、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或甘油-3-磷酸酰基转移酶。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、和/或天然甘油-3-磷酸酰基转移酶。
10.降低天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶的活性
在一些方面，本发明涉及一种转化的细胞，所述细胞包含遗传修饰，其中所述遗传修饰降低天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白的活性。在一些实施方案中，所述遗传修饰是敲除突变。
所述遗传修饰可以是用核酸转化。在某些实施方案中，本发明涉及一种改变细胞的脂质含量的方法，所述方法包括将所述细胞用降低所述细胞中天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白的活性的核酸转化。所述核酸可能能够与天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶基因和/或天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶基因的调节区中的核苷酸序列重组。在一些实施方案中，天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白是由GPT2基因编码的。
在某些实施方案中，所述细胞是酿酒酵母并且天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白具有SEQ ID NO:119中所示的氨基酸序列。在一些实施方案中，所述细胞是酿酒酵母，并且天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白是由SEQ ID NO:120中所示的核苷酸序列编码的。
在一些实施方案中，所述转化的细胞进一步包含提高以下各项的活性的遗传修饰：Δ9去饱和酶、延长酶、二酰基甘油酰基转移酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、磷脂:二酰基甘油酰基转移酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶(例如外源甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶)、和/或甘油-3-磷酸酰基转移酶。举例来说，所述转化的细胞可以包含(1)降低天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白的活性的遗传修饰；以及(2)由用编码外源甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白的核酸转化组成的遗传修饰。在一些实施方案中，所述转化的细胞进一步包含降低以下各项的活性的遗传修饰：天然Δ9去饱和酶、天然Δ12去饱和酶、天然二酰基甘油酰基转移酶、天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然磷脂酸磷酸酶、和/或天然甘油-3-磷酸酰基转移酶。
D.产品
在某些实施方案中，在外源脂肪酸、葡萄糖、乙醇、木糖、蔗糖、淀粉、淀粉糊精、甘油、纤维素和/或乙酸存在下培养所述转化的细胞。可以在培养期间添加这些底物以增加脂质产生。外源脂肪酸可以包括硬脂酸酯、油酸、亚油酸、γ-亚麻酸、二高-γ-亚麻酸、花生四烯酸、α-亚麻酸、十八碳四烯酸、二十碳四烯酸、二十碳五烯酸、二十二碳五烯酸、二十碳二烯酸、和/或二十碳三烯酸。
在某些实施方案中，本发明涉及一种产品，所述产品是由本文所述的修饰的宿主细胞产生的。在某些实施方案中，所述产品是油、脂质、或三酰基甘油。在一些实施方案中，所述产品是棕榈酸、棕榈油酸、硬脂酸、油酸、或亚油酸。在某些实施方案中，所述产品是饱和脂肪酸。因此，所述产品可以是辛酸、癸酸、月桂酸、肉豆蔻酸、棕榈酸、硬脂酸、花生酸、二十二烷酸、二十四烷酸、或蜡酸。在一些实施方案中，所述产品是不饱和脂肪酸。因此，所述产品可以是肉豆蔻脑酸、棕榈油酸、顺式-6-十六碳烯酸(sapienic acid)、油酸、反油酸、异油酸、亚油酸、反亚油酸、α-亚麻酸、花生四烯酸、二十碳五烯酸、芥酸、或二十二碳六烯酸。
在一些实施方案中，所述产品包含18-碳脂肪酸。在一些实施方案中，所述产品包含油酸、硬脂酸、或亚油酸。举例来说，所述产品可以是油酸。
本领域技术人员将容易了解的是，本发明很好地适用于实现所述目标并且获得所提到的目的和优势以及其中固有的那些。本文所述的实施方案不旨在作为对本发明的范围的限制。
实施例
通过以下实施例进一步说明本说明，所述实施例不应当以任何方式被视为构成限制。所有所引用的参考文献(包括如整个本申请中所引用的文献参考文献、授权专利、公开的专利申请、以及基因库登录号)的内容在此明确地以引用的方式并入本文。当以引用的方式并入本文的文件中术语的定义与本文所用的那些相矛盾时，以本文所用的定义为准。
实施例1：提高DGA1蛋白的活性的方法(DGAT2基因)。
用于过表达DGA1的示例性核酸构建体描述于USSN 61/943,664(在此以引用的方式并入本文)中。图2示出了用于使圆红冬孢酵母DGA1基因NG66(SEQ ID NO:22)在解脂耶氏酵母中过表达的表达构建体pNC243。在转化之前，通过PacI/NotI限制酶切消化将DGA1表达构建体线性化。线性表达构建体各自包括用于DGAT2基因和用于Nat1基因的表达盒，所述Nat1基因用作诺尔丝菌素(NAT)选择标记。
使用如Chen(Applied Microbiology&Biotechnology 48:232-35(1997))中所述的转化方案，使DGA1表达构建体随机整合到解脂耶氏酵母菌株NS18(从ARS培养物保藏中心获得，NRRL号：YB 392)的基因组中。在具有500μg/mL的NAT的YPD板上选择转化体。
对于大多数的构建体，转化体之间存在显著的菌落变异，这可能是因为在仅获得功能性Nat1盒的细胞中缺少功能性DGA1表达盒或是因为DGA1整合位点对DGA1表达的负面影响。所有的转化体均有脂质含量的显著增加。
在某些实验中，天然圆红冬孢酵母DGA1过表达对解脂耶氏酵母中的脂质产生的作用不如不含内含子的圆红冬孢酵母DGAT2基因的合成型式的作用那么高。这一结果可以表明在解脂耶氏酵母中圆红冬孢酵母DGAT2基因的基因剪接不是非常高效的。在某些实验中，针对在解脂耶氏酵母中的表达对圆红冬孢酵母DGA1基因进行的密码子优化对脂质产生没有积极的影响。
本领域技术人员将认识到的是，可以使用类似的方法来提高多种生物体中其它蛋白质的活性。
实施例2：降低天然三酰基甘油脂酶蛋白的活性的方法。
用于敲除解脂耶氏酵母TGL3基因，同时过表达DGAT2基因的示例性核酸构建体描述于WO 2015/168531和USSN 61/987,098(这两者均以引用的方式并入本文)中。将TGL3基因从解脂耶氏酵母野生型菌株NS18(从NRLL号YB-392获得)和它的DGA1过表达衍生物NS281中敲除。NS281过表达来自圆红冬孢酵母的DGA1基因，如上文所述。如下使解脂耶氏酵母TGL3基因(YALI0D17534g，SEQ ID NO:92)缺失：通过使用引物对NP1798-NP656和NP655-NP1799(SEQ ID NO:163-166)由含有潮霉素抗性基因(“hph”，SEQ ID NO:162)的质粒进行PCR来扩增两片段缺失盒。根据WO 2014/182657和USSN 61/819,746(这两者均以引用的方式并入本文)中开发的方案，将所得的PCR片段(SEQ ID NO:167和168)共同转化到NS18和NS281中。在hph盒中省去启动子和终止子以及将hph编码序列分成两个PCR片段降低了这些片段的随机整合将赋予潮霉素抗性的概率。hph基因应当只在它通过同源重组在TGL3基因座处整合以使得TGL3启动子和终止子可以引导它的转录的情况下才被表达。通过PCR以确认不存在TGL3和存在tgl3::hyg特异性产物来筛选潮霉素抗性菌落。
本领域技术人员将认识到的是，可以使用类似的方法来降低多种生物体中其它蛋白质的活性。
实施例3：降低解脂耶氏酵母中的Δ12去饱和酶活性。
Δ12去饱和酶经由将油酸去饱和，从而使C18:1脂肪酸转化成C18:2来引起亚油酸的产生。在解脂耶氏酵母菌株NS18中使Δ12基因(SEQ ID NO:2)缺失来产生菌株NS419。在NS419中诱导脂质积聚并且分析脂质组成。Δ12基因的缺失引起亚油酸产生的完全消除以及伴随的油酸增加(图3)。
实施例4：提高解脂耶氏酵母中的Δ9去饱和酶活性。
Δ9去饱和酶经由将硬脂酸去饱和，从而使C18:0脂肪酸转化成C18:1来引起油酸的产生。使Δ9基因(SEQ ID NO:4)在解脂耶氏酵母菌株NS18中过表达以产生菌株NS441。诱导脂质积聚并且分析脂质组成。Δ9基因的过表达引起油酸(和棕榈油酸)增加(图4)。
实施例5：提高解脂耶氏酵母中的延长酶活性。
延长酶使脂肪酸的碳链延长到超过由脂肪酸合酶产生的长度。YALI0F06754(SEQID NO:6)被鉴定为与酿酒酵母延长酶具有有限的同源性的解脂耶氏酵母基因。尽管这一基因没有被注释为延长酶，但是在解脂耶氏酵母中评估了它的功能，并且发现YALI0F06754在C16脂肪酸延长到C18脂肪酸中起作用。YALI0F06754因此被称作ELO1。
解脂耶氏酵母菌株NS18中ELO1的缺失引起C18水平的降低(图5A)。解脂耶氏酵母菌株NS18中ELO1的敲除被命名为NS276。相反，ELO1的过表达引起C18水平增加(图5B)，并且确切地说，ELO1的过表达增加了油酸水平(图5C)。在菌株NS452中进行ELO1过表达，所述菌株NS452过表达解脂耶氏酵母DGA1和紫色麦角菌DGA2并且含有Δ12去饱和酶缺失，从而产生菌株NS477。
实施例6：转换解脂耶氏酵母中的Δ9去饱和酶特异性。
解脂耶氏酵母的天然Δ9去饱和酶使用C16饱和脂肪酸和C18饱和脂肪酸这两者作为底物。通过引入基因(SEQ ID NO:8、10、12、14、以及16)作为解脂耶氏酵母中唯一的Δ9活性来针对更高的C18特异性筛选外源Δ9酶。这是通过首先使NS18中的解脂耶氏酵母Δ9缺失以产生菌株NS418来实现的。NS418由于不存在Δ9活性而需要补充不饱和脂肪酸，如油酸和/或Tween-80以生长。然后经由靶向整合将外源Δ9基因插入天然基因座中并且针对在没有补充的情况下生长的能力进行选择。来自在此所示的来源生物体的Δ9酶的表达在不存在天然酶的情况下使得底物特异性压倒性地转向C18:0底物，从而将C16:1含量减少到最低水平。来自噬腺嘌呤阿克苏拉酵母(SEQ ID NO:8)和禾柄锈菌(SEQ ID NO:14)的Δ9酶产生最高的油酸水平(图6)。
实施例7：降低解脂耶氏酵母中的酰基转移酶活性。
当天然酰基转移酶活性表现出对脂肪酸的底物偏好时，所述基因的缺失可能影响脂肪酸组成。在NS18中使甘油酰基转移酶SCT1(SEQ ID NO:18)缺失以产生菌株NS563。诱导脂质积聚并且分析脂质组成。SCT1的缺失引起油酸脂质分数的增加(图7)。
实施例8：提高解脂耶氏酵母中的酰基转移酶活性。
酰基转移酶的过表达可以提高总脂质水平以实现高脂质收率。重要的是，过表达的酰基转移酶具有所期望的底物特异性以维持或增加细胞的油酸含量。过表达可以是在野生型酰基转移酶背景下或在包含天然酰基转移酶的缺失的菌株中。使来自各种物种的2型二酰基甘油酰基转移酶(SEQ ID NO:20、22、24、26、28、以及30)在NS18中表达。来自解脂耶氏酵母的编码DGA1蛋白的DGAT2基因产生最高的油酸水平(图8)。类似地，使来自不同物种的1型二酰基甘油酰基转移酶(SEQ ID NO:32、34、36、38、以及40)在NS281(通过使圆红冬孢酵母DGA1在NS18中过表达所产生)中表达。来自紫色麦角菌的DGA2基因产生最高的油酸水平(图9)。此外，使来自不同物种的甘油-3-磷酸酰基转移酶(SEQ ID NO:18、42、44、46、以及48)在携带天然SCT1和Δ12基因缺失的菌株(NS564)中表达。来自酿酒酵母和噬腺嘌呤阿克苏拉酵母的SCT1基因产生最高的油酸水平(图10)。
实施例9：降低噬腺嘌呤阿克苏拉酵母中的Δ12去饱和酶活性。
使Δ12基因(SEQ ID NO:50)从噬腺嘌呤阿克苏拉酵母菌株NS252(ATCC 76597)中缺失以产生菌株NS478。诱导脂质积聚并且分析脂质组成。Δ12的缺失引起亚油酸产生的完全消除以及伴随的油酸增加(图11)。
实施例10：提高噬腺嘌呤阿克苏拉酵母中的酰基转移酶活性。
如在解脂耶氏酵母中那样，噬腺嘌呤阿克苏拉酵母中酰基转移酶的过表达可以提高总脂质水平以实现高脂质收率。重要的是，过表达的酰基转移酶具有所期望的底物特异性以维持或增加细胞的油酸含量。过表达可以是在野生型酰基转移酶背景下或在天然酰基转移酶缺失的菌株中。使来自不同物种的2型二酰基甘油酰基转移酶(SEQ ID NO:20、22、以及52)在噬腺嘌呤阿克苏拉酵母菌株NS252中表达。来自解脂耶氏酵母的DGA1基因(SEQ IDNO:20)产生最高的油酸水平(图12)。
实施例11：提高解脂耶氏酵母中的Δ9去饱和酶活性。
使解脂耶氏酵母Δ9去饱和酶基因在实施例5中所述的菌株NS477中过表达，从而产生菌株NS551。菌株NS551表达解脂耶氏酵母DGA1、紫色麦角菌DGA2、解脂耶氏酵母ELO1、解脂耶氏酵母Δ9，并且包含Δ12敲除。以占总C16和C18脂肪酸的百分比计，该菌株含有约87％的油酸。图13提供了菌株NS551的生物工程化的概述。
实施例12：提高噬腺嘌呤阿克苏拉酵母中的延长酶活性。
经由线性表达盒的随机基因组整合使来自噬腺嘌呤阿克苏拉酵母(AaELO1，SEQID NO:108；AaELO2，SEQ ID NO:160)、解脂耶氏酵母(YlELO1，SEQ ID NO:6)、酿酒酵母(ScELO1，SEQ ID NO:158)、以及褐家鼠(rELO2，SEQ ID NO:156)的延长酶基因在噬腺嘌呤阿克苏拉酵母菌株NS554中表达。NS554携带野生型噬腺嘌呤阿克苏拉酵母中的Δ12去饱和酶基因(SEQ ID NO:50)的缺失。AaELO1、YlELO1、以及rELO2的表达都增加了细胞的C18脂肪酸含量，这表明C16脂肪酸的延长增加(图14)。NS554中AaELO1、YlELO1、以及rELO2的表达还增加了油酸含量(图15)。
实施例13：转换解脂耶氏酵母中的延长酶特异性。
经由线性表达盒的随机基因组整合使来自噬腺嘌呤阿克苏拉酵母(AaELO1，SEQID NO:108；AaELO2，SEQ ID NO:160)、解脂耶氏酵母(YlELO1，SEQ ID NO:6)、酿酒酵母(ScELO1，SEQ ID NO:158)、以及褐家鼠(rELO2，SEQ ID NO:156)的延长酶基因在解脂耶氏酵母菌株NS276中表达。NS276携带野生型解脂耶氏酵母中的ELO1基因(SEQ ID NO:6)的缺失。rELO2的表达增加了细胞的C18脂肪酸含量，这表明C16脂肪酸的延长增加(图16)。此外，NS276中rELO2的表达还增加了油酸含量(图15)。
实施例14：提高噬腺嘌呤阿克苏拉酵母中的延长酶活性。
经由线性表达盒的随机基因组整合使来自解脂耶氏酵母(Yl ELO1，SEQ ID NO:6)的延长酶1基因在噬腺嘌呤阿克苏拉酵母菌株NS557中表达。NS557携带野生型噬腺嘌呤阿克苏拉酵母中的Δ12去饱和酶基因(SEQ ID NO:50)的缺失和DGA1的解脂耶氏酵母基因(SEQ ID NO:20)。大多数的转化体产生增加的C18脂肪酸，这表明C16脂肪酸由延长酶延长(图17)。筛选了总共95个分离株，并且图17描绘了代表性结果。表现最好的分离株被命名为NS776，并且进一步分析它的脂质组成。菌株NS776中C16脂肪酸和C18脂肪酸的约87％是油酸(图18)。
实施例15：解脂耶氏酵母中遗传修饰的组合。
将遗传修饰的各种组合引入到解脂耶氏酵母中。用于引入一些修饰的策略示于图19中。使用野生型解脂耶氏酵母菌株NS18作为亲本菌株(从ARS培养物保藏中心获得，NRRL号YB 392)。通过首先使SCT1基因(SEQ ID NO:18)缺失，然后添加来自噬腺嘌呤阿克苏拉酵母的SCT1基因(SEQ ID NO:44)由菌株NS18制备菌株NS804。通过首先使Δ9去饱和酶基因(SEQ ID NO:4)缺失，然后添加来自禾柄锈菌的Δ9去饱和酶基因(SEQ ID NO:14)由菌株NS804制备菌株NS809。通过首先使Δ9去饱和酶基因(SEQ ID NO:4)缺失，然后添加来自噬腺嘌呤阿克苏拉酵母的Δ9去饱和酶基因(SEQ ID NO:8)由菌株NS804制备菌株NS810。
通过使Δ12去饱和酶基因(SEQ ID NO:2)缺失，使来自解脂耶氏酵母的DGA1基因(SEQ ID NO:20)过表达，添加来自紫色麦角菌的DGA2基因(SEQ ID NO:38)，并且添加来自褐家鼠的ELO2基因(SEQ ID NO:156)由菌株NS18制备菌株NS813。
通过使Δ12去饱和酶基因(SEQ ID NO:2)缺失，使Δ9去饱和酶基因(SEQ ID NO:4)缺失，添加来自噬腺嘌呤阿克苏拉酵母的Δ9去饱和酶基因(SEQ ID NO:8)，使SCT1基因(SEQ ID NO:18)缺失，添加来自噬腺嘌呤阿克苏拉酵母的SCT1基因(SEQ ID NO:44)，并且添加来自褐家鼠的ELO2基因(SEQ ID NO:156)由菌株NS18制备菌株NS814。
菌株NS18、NS804、NS809、NS810、NS813、以及NS814的脂肪酸谱示于图20中。每一种修饰的菌株均比野生型菌株产生更多的油酸(C18:1)。
通过使Δ12基因(SEQ ID NO:2)缺失由菌株NS809制备菌株NS968。通过添加来自褐家鼠的ELO2基因(SEQ ID NO:156)并且添加来自圆红冬孢酵母的DGA1基因(SEQ ID NO:22)由菌株NS968制备菌株NS975。菌株NS992、NS993、以及NS994是通过添加来自禾柄锈菌的Δ9去饱和酶基因(SEQ ID NO:14)，添加来自圆红冬孢酵母的DGA1基因(SEQ ID NO:22)的另外的拷贝，并且添加来自紫色麦角菌的DGA2基因(SEQ ID NO:38)由菌株NS975制备的三种分离株。
通过使Δ12基因(SEQ ID NO:2)缺失由菌株NS810制备菌株NS812。通过添加来自禾柄锈菌的Δ9去饱和酶基因(SEQ ID NO:14)，添加来自圆红冬孢酵母的DGA1基因(SEQ IDNO:22)，添加来自褐家鼠的ELO2基因(SEQ ID NO:156)，并且添加来自紫色麦角菌的DGA2基因(SEQ ID NO:38)由菌株NS812制备菌株NS969。
通过使Δ12基因(SEQ ID NO:2)缺失，使SCT1基因(SEQ ID NO:18)缺失，并且添加来自噬腺嘌呤阿克苏拉酵母的SCT1基因(SEQ ID NO:44)由菌株NS18制备菌株NS662。
菌株NS18、NS804、NS809、NS968、NS975、NS992、NS993、NS994、NS810、NS812、NS969、NS987、NS988、NS551(描述于实施例11中)、以及NS622的脂肪酸谱示于图21中。每一种修饰的菌株均比野生型菌株产生更多的油酸(C18:1)。此外，用来自紫色麦角菌的DGA2基因(SEQID NO:38)修饰的每一种菌株均比野生型NS18菌株包含更多的脂质。
以引用的方式并入
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等同方案
本领域技术人员仅使用常规的实验就将认识到或能够确定在本文所描述的本发明的具体实施方案的多个等同方案。这些等同方案意在由以下权利要求书所涵盖。
序列表
<110> 诺沃吉公司
<120> 酵母中的油酸生产
<130> NGX-037.25
<140>
<141>
<150> 62/090,169
<151> 2014-12-10
<160> 168
<170> PatentIn 3.5版
<210> 1
<211> 419
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<213> 解脂耶氏酵母
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gatgtcaact acaaggtcaa gtacacctcc ggcgttaaga tgagccaggg cgcctacgac 120
gacaagggcc gccacatttc cgagcagccc ttcacctggg ccaactggca ccagcacatc 180
aactggctca acttcattct ggtgattgcg ctgcctctgt cgtcctttgc tgccgctccc 240
ttcgtctcct tcaactggaa gaccgccgcg tttgctgtcg gctattacat gtgcaccggt 300
ctcggtatca ccgccggcta ccaccgaatg tgggcccatc gagcctacaa ggccgctctg 360
cccgttcgaa tcatccttgc tctgtttgga ggaggagctg tcgagggctc catccgatgg 420
tgggcctcgt ctcaccgagt ccaccaccga tggaccgact ccaacaagga cccttacgac 480
gcccgaaagg gattctggtt ctcccacttt ggctggatgc tgcttgtgcc caaccccaag 540
aacaagggcc gaactgacat ttctgacctc aacaacgact gggttgtccg actccagcac 600
aagtactacg tttacgttct cgtcttcatg gccattgttc tgcccaccct cgtctgtggc 660
tttggctggg gcgactggaa gggaggtctt gtctacgccg gtatcatgcg atacaccttt 720
gtgcagcagg tgactttctg tgtcaactcc cttgcccact ggattggaga gcagcccttc 780
gacgaccgac gaactccccg agaccacgct cttaccgccc tggtcacctt tggagagggc 840
taccacaact tccaccacga gttcccctcg gactaccgaa acgccctcat ctggtaccag 900
tacgacccca ccaagtggct catctggacc ctcaagcagg ttggtctcgc ctgggacctc 960
cagaccttct cccagaacgc catcgagcag ggtctcgtgc agcagcgaca gaagaagctg 1020
gacaagtggc gaaacaacct caactggggt atccccattg agcagctgcc tgtcattgag 1080
tttgaggagt tccaagagca ggccaagacc cgagatctgg ttctcatttc tggcattgtc 1140
cacgacgtgt ctgcctttgt cgagcaccac cctggtggaa aggccctcat tatgagcgcc 1200
gtcggcaagg acggtaccgc tgtcttcaac ggaggtgtct accgacactc caacgctggc 1260
cacaacctgc ttgccaccat gcgagtttcg gtcattcgag gcggcatgga ggttgaggtg 1320
tggaagactg cccagaacga aaagaaggac cagaacattg tctccgatga gagtggaaac 1380
cgaatccacc gagctggtct ccaggccacc cgggtcgaga accccggtat gtctggcatg 1440
gctgcttag 1449
<210> 5
<211> 304
<212> PRT
<213> 解脂耶氏酵母
<400> 5
Met Leu Ser Ser Ile Ser Pro Asp Leu Tyr Ser Ser Phe Ser Phe Lys
1 5 10 15
Asn Ser Leu Ala Glu Ala Met Pro Ser Val Pro His Glu Leu Ile Asn
20 25 30
Ser Lys Thr Leu Ser Trp Met Tyr Asn Ala Ser Leu Asp Ile Arg Val
35 40 45
Pro Leu Thr Ile Gly Thr Ile Tyr Ala Val Ser Val His Leu Thr Asn
50 55 60
Ser Ser Glu Arg Ile Lys Lys Arg Gln Pro Ile Ala Phe Ala Lys Thr
65 70 75 80
Ala Leu Phe Lys Trp Leu Cys Val Leu His Asn Ala Gly Leu Cys Leu
85 90 95
Tyr Ser Ala Trp Thr Phe Val Gly Ile Leu Asn Ala Val Lys His Ala
100 105 110
Tyr Gln Ile Thr Gly Asp Ser Ser Ala Pro Phe Ser Phe Asn Thr Leu
115 120 125
Trp Gly Ser Phe Cys Ser Arg Asp Ser Leu Trp Val Thr Gly Leu Asn
130 135 140
Tyr Tyr Gly Tyr Trp Phe Tyr Leu Ser Lys Phe Tyr Glu Val Val Asp
145 150 155 160
Thr Met Ile Ile Leu Ala Lys Gly Lys Pro Ser Ser Met Leu Gln Thr
165 170 175
Tyr His His Thr Gly Ala Met Phe Ser Met Trp Ala Gly Ile Arg Phe
180 185 190
Ala Ser Pro Pro Ile Trp Ile Phe Val Val Phe Asn Ser Leu Ile His
195 200 205
Thr Ile Met Tyr Phe Tyr Tyr Thr Leu Thr Thr Leu Lys Ile Lys Val
210 215 220
Pro Lys Ile Leu Lys Ala Ser Leu Thr Thr Ala Gln Ile Thr Gln Ile
225 230 235 240
Val Gly Gly Gly Ile Leu Ala Ala Ser His Ala Phe Ile Tyr Tyr Lys
245 250 255
Asp His Gln Thr Glu Thr Val Cys Ser Cys Leu Thr Thr Gln Gly Gln
260 265 270
Phe Phe Ala Leu Ala Val Asn Val Ile Tyr Leu Ser Pro Leu Ala Tyr
275 280 285
Leu Phe Ile Ala Phe Trp Ile Arg Ser Tyr Leu Lys Ala Lys Ser Asn
290 295 300
<210> 6
<211> 915
<212> DNA
<213> 解脂耶氏酵母
<400> 6
atgctctcgt caatctcgcc cgacctatac tcgtccttct cgttcaaaaa ctcgctcgcc 60
gaggccatgc cctccgtgcc acacgaactc atcaactcaa aaacactctc atggatgtac 120
aatgcctctc tggacattcg ggttcctctg actatcggaa ccatctacgc cgtctccgtg 180
cacctgacca actcatctga acgaatcaag aaacgccagc ccattgcctt tgccaagacc 240
gcactcttca agtggctctg tgtcctccac aatgcaggtc tgtgtctcta ctcagcatgg 300
acctttgtcg gtatcctcaa cgccgtcaaa cacgcctacc aaatcacagg agacagctcc 360
gcccccttct ccttcaacac cctctgggga tcgttttgtt cacgtgactc cctctgggtc 420
accggcctca actactacgg atactggttc tatctgtcca aattctacga agtggtggac 480
accatgatca tcctcgcaaa gggaaaaccg tcctcaatgc tccagacata ccaccacacc 540
ggcgccatgt tctccatgtg ggccggcatc cgattcgcct ctccccccat ctggatcttt 600
gtggttttca actccctcat ccacacaatc atgtactttt actacaccct caccaccctc 660
aagatcaagg ttcccaagat cctcaaggca tctctgacca ccgcccagat cacccagatt 720
gtcggaggtg gcatcctggc tgcctcccac gcctttattt attacaagga ccaccagact 780
gagaccgtct gttcttgtct cactacccag ggtcagtttt tcgctctcgc cgtcaatgtc 840
atctatctga gtcctctggc ctatctcttt attgccttct ggattcgatc ttacttgaag 900
gccaagtcca actag 915
<210> 7
<211> 486
<212> PRT
<213> 噬腺嘌呤阿克苏拉酵母
<400> 7
Met Asn Gly Pro Glu Glu Val Asn Leu Glu Glu Val Gln Ala Ile Ala
1 5 10 15
Ser Gly Ala Glu Val Arg Ala Lys Val Asn Ile Asn Arg Arg Arg Gln
20 25 30
Glu Glu Gln Ala Ala Ala Ala Ala Ala Ser Ser Gly Ser Thr Lys Thr
35 40 45
His Ile Ser Glu Gln Ala Phe Thr Leu Ala Asn Trp His Lys His Phe
50 55 60
Asn Trp Ile Asn Thr Thr Ile Ile Ala Ile Ile Pro Ala Ile Gly Phe
65 70 75 80
Leu Ser Val Pro Phe Ile Pro Val His Gly Lys Thr Leu Ala Trp Ala
85 90 95
Phe Val Tyr Tyr Phe Leu Thr Gly Leu Gly Ile Thr Ala Gly Tyr His
100 105 110
Arg Leu Trp Ala His Arg Ala Tyr Ser Ala Ser Trp Pro Leu Arg Val
115 120 125
Phe Leu Ala Leu Leu Gly Ala Gly Ala Gly Glu Gly Ser Val Lys Trp
130 135 140
Trp Ser Asn Gly His Arg Thr His His Arg Tyr Thr Asp Thr Asp Lys
145 150 155 160
Asp Pro Tyr Asn Ala Lys Arg Gly Phe Trp Phe Ser His Met Gly Trp
165 170 175
Met Met Phe Lys Gln Asn Pro Lys Leu Lys Gly Arg Cys Asp Ile Ser
180 185 190
Asp Leu Ile Cys Asp Pro Ile Ile Arg Trp Gln His Arg His Tyr Ile
195 200 205
Trp Ile Met Ala Ala Met Ser Phe Val Phe Pro Ser Val Val Ala Gly
210 215 220
Leu Gly Trp Gly Asp Tyr Leu Gly Gly Phe Val Phe Ala Gly Ile Leu
225 230 235 240
Arg Gln Phe Val Val His Gln Ser Thr Phe Cys Val Asn Ser Leu Ala
245 250 255
His Trp Leu Gly Glu Gln Pro Phe Asp Asp Asn Arg Ser Pro Arg Asp
260 265 270
His Val Leu Thr Ala Phe Ala Thr Leu Gly Glu Gly Tyr His Asn Phe
275 280 285
His His Glu Phe Pro Ser Asp Tyr Arg Asn Ala Ile Lys Trp Tyr Gln
290 295 300
Tyr Asp Pro Thr Lys Ile Phe Ile Trp Thr Met Lys Gln Leu Gly Leu
305 310 315 320
Ala Ser Asn Leu Gln Thr Phe Ser Gln Asn Ala Ile Glu Gln Gly Leu
325 330 335
Val Gln Gln Lys Gln Lys Lys Leu Asp Arg Trp Arg Ala Arg Leu Asn
340 345 350
Trp Gly Val Pro Ile Glu Gln Leu Pro Val Ile Glu Tyr Asp Asp Phe
355 360 365
Lys Asp Glu Ser Ser Ser Arg Ser Leu Val Leu Ile Ser Gly Ile Val
370 375 380
His Asp Val Thr Asp Phe Ile Asp Lys His Pro Gly Gly Lys Ala Leu
385 390 395 400
Ile Lys Ser Ala Ile Gly Lys Asp Gly Thr Ala Val Phe Asn Gly Gly
405 410 415
Val Tyr Lys His Ser Asn Ala Ala His Asn Leu Leu Ala Thr Met Arg
420 425 430
Val Ala Val Ile Arg Gly Gly Met Glu Val Glu Val Trp Lys Arg Ala
435 440 445
Gln Gly Glu Lys Lys Asp Val Asp Pro Val Ala Asp Ser Ala Gly Asp
450 455 460
Arg Ile Leu Arg Ala Gly Asp Gln Pro Ser Arg Val Pro Glu Ala Arg
465 470 475 480
Val Ser Gly Arg Ala Ala
485
<210> 8
<211> 1461
<212> DNA
<213> 噬腺嘌呤阿克苏拉酵母
<400> 8
atgaacggtc ccgaagaggt gaatctcgaa gaagtccagg ccattgcgtc tggagccgaa 60
gttcgagcta aggtcaacat caaccgacga aggcaagagg agcaggctgc cgccgctgct 120
gcctccagcg gttcgacaaa gactcatatc tccgagcagg ctttcaccct cgccaactgg 180
cacaagcatt tcaattggat caacacaacc atcattgcca ttatcccagc aatcggcttt 240
ttgtcggtgc ctttcattcc tgtgcacggc aagacattag cgtgggcatt cgtctactac 300
tttttgaccg gtctgggaat caccgccgga taccaccgtc tgtgggctca ccgggcttac 360
agtgcatcat ggcccctgcg agtgttcttg gcacttttgg gtgctggagc cggtgagggt 420
tcagtaaagt ggtggtctaa tggacaccgc actcaccacc gttacactga cactgacaag 480
gatccttaca atgccaagcg aggattctgg ttctcccaca tgggctggat gatgttcaag 540
cagaacccca agctcaaggg acgatgcgac atttccgatc ttatctgcga ccctattatt 600
cgatggcagc accgacacta catttggatc atggcagcaa tgtcgtttgt attcccttct 660
gtagttgctg gactgggctg gggagactac ctgggaggat ttgtgtttgc aggaatcctg 720
cgacagtttg ttgtccacca gtcgaccttc tgtgtcaact cgcttgccca ctggctggga 780
gagcagcctt ttgacgacaa ccgatctcct cgagaccacg ttctgactgc gtttgctact 840
ctgggtgagg gttaccacaa cttccaccac gagttcccct ccgactaccg taacgctatc 900
aagtggtacc agtacgaccc taccaagatc ttcatctgga ccatgaagca gcttggtctg 960
gcctctaacc tgcagacttt ctcccagaat gctattgagc agggtctggt acagcagaag 1020
cagaagaagc tggaccgatg gagagctcgt ctcaactggg gagtgcctat tgagcagctt 1080
cctgtaattg agtacgacga cttcaaggac gagtcatctt cccgatcttt ggtcctcatt 1140
tctggaattg tccacgatgt taccgacttt attgacaagc accctggtgg aaaggctctc 1200
atcaagagcg ccattggcaa ggacggaact gccgtgttca acggaggtgt gtacaagcac 1260
tccaacgctg ctcacaacct gctggccact atgcgtgtag ctgtcattcg aggaggaatg 1320
gaagtcgagg tctggaagcg tgcccagggc gagaagaagg atgttgaccc tgtcgccgat 1380
tccgctggtg accgtatcct gcgagctggc gaccagcctt ctcgtgttcc tgaggcccgt 1440
gtctctggcc gcgctgctta a 1461
<210> 9
<211> 545
<212> PRT
<213> 圆红冬孢酵母
<400> 9
Met Thr Ala Ser Ser Ala Leu Glu Thr Ser Leu Pro His Ser Val Gly
1 5 10 15
Pro Glu Ala Ala Thr Thr Thr Ala Lys Pro Pro Arg Ala Pro Leu Arg
20 25 30
Met Arg His Pro Asp Tyr Thr Gln Thr Asp Val Leu Asp Ser Ser Asp
35 40 45
Ser Asp Ala Ala Ser Asp Ser Glu Gly Glu Thr Thr Ala Val Asp Asp
50 55 60
Gly Thr Tyr Glu Asp Asp Asn Tyr Val Arg Lys Val Leu Ser Lys Glu
65 70 75 80
Lys Pro Leu Pro Pro Ile Thr Trp Lys Asn Ile His Arg Asn Ile Gln
85 90 95
Trp Ile Ser Thr Leu Ala Leu Thr Ile Val Pro Leu Leu Ala Ile Tyr
100 105 110
Gly Ala Phe Thr Thr Pro Leu Lys Trp Gln Thr Ala Val Trp Ser Val
115 120 125
Val Tyr Tyr Tyr Tyr Thr Gly Leu Gly Ile Thr Ala Gly Tyr His Arg
130 135 140
Leu Trp Ala His Arg Ser Tyr Thr Ala Ser Leu Pro Leu Gln Tyr Phe
145 150 155 160
Leu Ala Leu Gly Gly Ser Gly Ala Val Glu Gly Ser Val Lys Trp Trp
165 170 175
Ser Arg Gly His Arg Ala His His Arg Tyr Thr Asp Thr Asp Leu Asp
180 185 190
Pro Tyr Ser Ala Gln Lys Gly Phe Trp Trp Ala His Leu Gly Trp Met
195 200 205
Ile Val Lys Pro Arg Arg Arg Pro Gly Val Ala Asp Val Ser Asp Leu
210 215 220
Asn Asn Asn Pro Val Val Lys Trp Gln His Arg Tyr Tyr Leu Pro Leu
225 230 235 240
Ile Leu Gly Met Gly Phe Val Phe Pro Thr Ile Val Ala Gly Leu Gly
245 250 255
Trp Gly Asp Phe Arg Gly Gly Phe Phe Phe Ala Gly Ala Ala Arg Leu
260 265 270
Leu Phe Val His His Ser Thr Phe Cys Val Asn Ser Leu Ala His Trp
275 280 285
Leu Gly Glu Thr Pro Phe Asp Asp Lys His Thr Pro Lys Asp His Trp
290 295 300
Leu Thr Ala Leu Ala Thr Val Gly Glu Gly Tyr His Asn Phe His His
305 310 315 320
Glu Phe Pro Ser Asp Tyr Arg Asn Ala Leu Arg Trp Trp Gln Tyr Asp
325 330 335
Pro Thr Lys Leu Phe Ile Trp Thr Met Ser Lys Leu Gly Leu Ala Ser
340 345 350
Gln Leu Lys Thr Phe Pro Asp Asn Glu Ile Lys Lys Gly Gln Tyr Ala
355 360 365
Met Thr Leu Lys Ala Val Ala Arg Glu Ala Glu Asn Ile Glu Trp Pro
370 375 380
Lys Ser Ser Asn His Leu Pro Val Leu Thr Trp Asp Glu Phe Gln Asp
385 390 395 400
Ala Cys Lys Thr Arg Gln Leu Leu Val Val Ala Gly Phe Ile His Asp
405 410 415
Val Ser Thr Phe Ile Asp Gln His Pro Gly Gly Ala Gly Leu Ile Lys
420 425 430
Thr Arg Leu Gly Arg Asp Ala Thr Thr Ala Phe Tyr Gly Gly Tyr Tyr
435 440 445
Asp His Ser Asn Gly Ala Ala Asn Leu Leu Ala Gln Tyr Arg Val Gly
450 455 460
Val Ile Glu Gly Gly Tyr Glu Val Glu His Met Lys Lys Tyr Ser Glu
465 470 475 480
Val Val Glu Asn Leu Lys Lys His Gly Ala Asp Gly Val Ala Gly Lys
485 490 495
Ser Ala Asp Leu Val Lys Gly Pro Lys Gln Thr Ser Val Ile Lys Gly
500 505 510
Asp Pro Gln Leu Lys Ser Ala Pro Leu Glu Thr Leu Ala Lys Pro Pro
515 520 525
Thr Phe Ser Glu Thr Asn Leu Leu Gly Gly Leu Ser Leu Lys Val Lys
530 535 540
Ala
545
<210> 10
<211> 1638
<212> DNA
<213> 圆红冬孢酵母
<400> 10
atgactgcct cttcggcact cgagacctcg ctcccgcact ctgtcgggcc cgaggctgcg 60
accaccacag caaagccgcc ccgtgcgccg ctcaggatgc gtcaccccga ctacactcag 120
accgacgtcc tcgattcgtc ggactcggat gcagcgtcgg attctgaggg cgagacgacg 180
gcggtcgacg atgggaccta cgaggacgac aactacgtcc gcaaggtcct cagcaaggag 240
aagccgctcc cgcccatcac ctggaagaac atccaccgca acatccagtg gatctcgacc 300
ctcgccctca ccatcgtgcc cctcctcgcc atctacggag cgttcacgac gcccttgaag 360
tggcagacgg cggtctggag tgtcgtctac tactactaca ccggacttgg tatcacggca 420
ggctaccaca ggctgtgggc ccacaggtcc tacaccgcct ctctgcctct ccagtacttc 480
ttggcacttg gaggaagcgg cgcagtcgag gggagcgtga aatggtggtc taggggacac 540
cgcgcacacc atcgctacac cgacacagac ctcgacccgt attcggcgca gaagggcttc 600
tggtgggctc accttggctg gatgatcgtc aagccgcgcc gtcgtcccgg tgtcgccgac 660
gtttccgacc tcaacaacaa cccggtcgtc aagtggcagc accgctacta tctcccgctc 720
atcctcggca tgggcttcgt cttccctacc atcgtcgctg gactcggctg gggcgacttc 780
cgcggcggat tcttcttcgc cggcgccgct cgcctcctct tcgtccacca ctcgacgttc 840
tgcgtcaact cgcttgcgca ctggctgggc gagacgccct tcgacgacaa gcacacgccg 900
aaggatcact ggctcacggc gctcgcgact gtcggtgagg gctaccacaa cttccaccac 960
gagttcccct ccgactaccg caacgcactt cgatggtggc agtacgaccc gactaagctc 1020
ttcatctgga cgatgtcgaa gctcggattg gcgtcgcagc tcaagacgtt ccccgacaac 1080
gagatcaaga agggccagta cgcgatgacg ctcaaggctg tcgcgcgcga ggccgagaac 1140
atcgagtggc ccaagtcgtc gaaccatttg cctgtgctca cttgggacga gttccaggac 1200
gcctgcaaga cccgtcagct ccttgttgtc gccggtttca tccacgatgt cagcacgttc 1260
atcgaccagc accccggcgg tgccggcttg atcaagactc gtctcggtcg cgacgcgacg 1320
actgccttct acggtggcta ctacgaccac tcgaacggcg cagccaacct gctcgcccag 1380
taccgtgtcg gtgtcatcga aggcggctac gaggtcgagc acatgaagaa gtattcggag 1440
gtcgtcgaga acctcaagaa gcacggcgcc gatggcgtgg ccggcaagag cgccgacctc 1500
gtcaagggcc cgaagcagac gtcggtcatc aagggcgacc ctcagctgaa gagcgcgccg 1560
ctcgagacgc tcgccaagcc gcccaccttc agcgaaacca acctcttggg cggtctcagc 1620
ctgaaggtca aggcgtaa 1638
<210> 11
<211> 546
<212> PRT
<213> 花药黑粉菌
<400> 11
Met Ser Val Thr Ala Ser Ala Ser His Lys Leu Ala Ala Ser Leu Pro
1 5 10 15
His Gly Asp Gly Gln Val Ser Ala Ser Thr Thr Ala Arg Ser Thr Ala
20 25 30
Pro Leu Arg Met Arg His Pro Asp Lys Gly Glu Ala Ser Asp Ser Asp
35 40 45
Ser Asp His Gly Thr Asp Ser Asp Gly Glu Thr Thr Ala Val Asp Asp
50 55 60
Gly Tyr Ala Glu Asp Asn Tyr Val Arg Lys Val Leu Ala Lys Glu Arg
65 70 75 80
Pro Leu Pro Pro Ile Thr Leu Lys Thr Leu Pro Gln Asn Ile Gln Val
85 90 95
Val Ser Thr Leu Ala Leu Thr Leu Val Pro Leu Leu Ala Val Tyr Gly
100 105 110
Ala Phe Thr Thr Glu Ile Lys Trp Gln Thr Met Val Trp Ser Val Val
115 120 125
Tyr Tyr Phe Phe Thr Gly Leu Gly Ile Thr Ala Gly Tyr His Arg Leu
130 135 140
Trp Ala His Arg Ser Tyr Thr Ala Ser Arg Pro Leu Gln Tyr Phe Leu
145 150 155 160
Ala Leu Gly Gly Ser Gly Ala Val Glu Gly Ser Ile Lys Trp Trp Ala
165 170 175
Arg Gly His Arg Ala His His Arg Tyr Thr Asp Thr Asp Leu Asp Pro
180 185 190
Tyr Ser Ala Gln Lys Gly Phe Leu His Ala His Leu Leu Trp Met Val
195 200 205
Leu Lys Pro Arg Arg Ala Pro Gly Thr Ala Asp Val Ser Asp Leu Ser
210 215 220
Ser Asn Glu Val Val Lys Trp Gln His Arg Phe Tyr Leu Pro Leu Ile
225 230 235 240
Val Gly Met Gly Phe Val Phe Pro Thr Val Val Ala Gly Leu Gly Trp
245 250 255
Gly Asp Trp Arg Gly Gly Tyr Phe Phe Ala Gly Ala Ala Arg Leu Thr
260 265 270
Phe Val His His Ser Thr Phe Cys Val Asn Ser Leu Ala His Trp Leu
275 280 285
Gly Glu Thr Pro Phe Asp Asp Lys His Thr Pro Lys Asp His Phe Leu
290 295 300
Thr Ala Leu Val Thr Val Gly Glu Gly Tyr His Asn Phe His His Glu
305 310 315 320
Phe Pro Ser Asp Phe Arg Asn Ala Ile Lys Trp Tyr Gln Tyr Asp Pro
325 330 335
Thr Lys Trp Phe Ile Phe Thr Met Tyr Lys Leu Gly Leu Ala Ser Asn
340 345 350
Leu Gln Thr Phe Pro Asp Ser Glu Leu Ala Arg Gly Gln Phe Asn Met
355 360 365
Lys Leu Lys Lys Leu Ala Ala Ser Ala Ser Asn Leu Pro Trp Pro Lys
370 375 380
Ser Ser Asn Asp Leu Pro Val Leu Thr Trp Glu Ser Phe Gln Glu Glu
385 390 395 400
Ala Lys Ser Arg Asp Leu Leu Val Ile Gly Gly Phe Ile His Asp Val
405 410 415
Ser Gln Phe Met Asp Asp His Pro Gly Gly Arg Gly Leu Ile Lys Ser
420 425 430
Arg Leu Gly Arg Asp Ala Thr Thr Ala Phe Tyr Gly Gly Tyr Tyr Asp
435 440 445
His Ser Asn Ala Ala Gly Asn Val Leu Ala Arg Leu Arg Val Gly Cys
450 455 460
Ile Glu Gly Gly Tyr Glu Val Glu Ala Leu Lys Lys Tyr Ser Gln Ile
465 470 475 480
Ile Glu Asp Ile Lys Arg Tyr Gly Gly Asp Gly Val Ala Gly Lys Thr
485 490 495
Ala Asp Met Gly Ser Ser Ala Arg Ala Thr Val Ala Ile Lys Gly Asp
500 505 510
Pro Ala His Arg Gly Ser Lys Leu Asp Thr Leu Lys Asp Tyr Pro Thr
515 520 525
Phe Lys Pro Glu Val Leu Pro Leu Asn Gly Gly Leu Thr Gln Ser Leu
530 535 540
Phe Glu
545
<210> 12
<211> 1641
<212> DNA
<213> 花药黑粉菌
<400> 12
atgtccgtca cggcctctgc tagtcacaag cttgccgcct cgctgcccca tggcgacggt 60
caggttagtg cttcgaccac cgcgcgctcg acagccccgc ttcgcatgcg ccacccagat 120
aagggtgaag cttccgactc ggactcggac catggtaccg acagcgacgg cgaaacgact 180
gccgtcgacg acgggtacgc tgaggacaac tatgtccgca aggttctcgc caaggaacga 240
cctctgccgc ccatcacgct caagactttg ccacagaaca tccaagtcgt ttcgactctc 300
gcgttgacac tagtgccgct cctggccgtc tacggtgcct ttacgacgga gattaagtgg 360
caaacgatgg tatggtctgt tgtttactac ttcttcaccg gccttggcat taccgcgggc 420
tatcatcgac tctgggcgca ccgatcttac actgcctcga ggccgctgca gtactttttg 480
gcgcttggtg gctcgggtgc tgtcgaaggt tcaatcaaat ggtgggcacg tggccaccgc 540
gcgcatcatc gctacaccga caccgacctg gacccgtact ccgcgcagaa gggcttcctt 600
cacgcccact tgctttggat ggtcctcaag cctcgccgtg caccgggaac cgccgacgtt 660
tctgatttgt cgtcgaacga agtcgtcaag tggcagcatc ggttttacct ccccctcatt 720
gtcggcatgg gctttgtctt ccccactgtg gtggccggac tgggttgggg agactggcgc 780
ggaggctact tctttgcggg tgcggctcga cttaccttcg tccatcactc gaccttctgc 840
gtcaactcct tggcgcattg gcttggcgaa actccttttg atgacaagca cacgcccaag 900
gaccacttcc tcactgcgct cgtcaccgtc ggcgagggat accacaactt ccaccacgag 960
tttccctcgg acttccgcaa tgccatcaaa tggtaccagt acgatccgac taagtggttt 1020
atcttcacca tgtacaagct tggtctggcg tcaaatcttc aaacattccc cgattcggaa 1080
ctcgcgcggg gtcaattcaa catgaagctg aagaaacttg ctgcctccgc gtcgaatctc 1140
ccttggccca agagctccaa cgatcttccc gttcttacgt gggaatcatt ccaggaagag 1200
gccaaatcgc gcgatttgct cgtcattggc ggcttcattc atgacgtttc gcagtttatg 1260
gatgaccatc ccggtggacg aggcctgatc aaaagtcgtc ttggtcgcga cgccactacc 1320
gcgttctacg gcgggtacta cgaccacagc aatgccgctg gcaacgtact tgcccgattg 1380
cgtgtcggct gcatcgaagg tggctatgaa gtagaggcgc tcaagaagta ttctcaaatc 1440
atcgaagaca taaagagata cggcggtgat ggtgtggctg gcaagactgc tgatatgggc 1500
tcctccgctc gtgctacggt ggccatcaaa ggtgatcctg cgcaccgcgg gtcgaagctg 1560
gacaccctca aggactaccc cactttcaag cccgaggttc tccctttgaa tggcggttta 1620
acccagtccc tctttgaata g 1641
<210> 13
<211> 507
<212> PRT
<213> 禾柄锈菌
<400> 13
Met Ser Lys Pro Ser Pro Ser Thr Pro Ala Thr Ala Pro His Leu Arg
1 5 10 15
Gln Arg Gln Arg Lys Asn Leu Pro Asp Tyr Asp Pro Asp Ser Asp Leu
20 25 30
Ser Glu Ser Glu Gly Leu Gly Gly Leu Arg Ser Gln Val Gly Asn Thr
35 40 45
Trp Glu Asp Asp Glu Glu Thr Ala Val Asp Asp Asp Ser Tyr Val Gln
50 55 60
Arg Thr Leu Arg Lys Glu Lys Pro Leu Pro Pro Ile Thr Trp Cys Asn
65 70 75 80
Phe Tyr Arg Glu Ile Asn Met Ile Ser Thr Leu Ala Leu Thr Val Val
85 90 95
Pro Ile Leu Ala Ile Tyr Gly Ala Phe Thr Thr Pro Leu Tyr Arg Ser
100 105 110
Thr Leu Ala Trp Ser Ile Leu Tyr Tyr Tyr Phe Thr Gly Leu Gly Ile
115 120 125
Thr Ala Gly Tyr His Arg Leu Trp Ala His Arg Ser Tyr Asn Ala Ser
130 135 140
Leu Pro Leu Gln Tyr Phe Leu Ala Leu Gly Gly Ser Gly Ala Val Glu
145 150 155 160
Gly Ser Ile Arg Trp Trp Ala Arg Gly His Arg Ala His His Arg Tyr
165 170 175
Thr Asp Thr Asp Leu Asp Pro Tyr Ser Ala His Lys Gly Leu Leu Trp
180 185 190
Ser His Val Gly Trp Met Ile Val Lys Pro Arg Arg Lys Pro Gly Val
195 200 205
Ala Asp Val Ser Asp Leu Ser Arg Asn Gln Val Val Arg Trp Gln His
210 215 220
Arg Trp Tyr Leu Pro Leu Ile Phe Gly Met Gly Phe Phe Phe Pro Thr
225 230 235 240
Leu Val Ala Gly Leu Gly Trp Gly Asp Trp Arg Gly Gly Phe Phe Tyr
245 250 255
Ala Gly Ala Ala Arg Leu Leu Phe Val His His Ser Thr Phe Cys Val
260 265 270
Asn Ser Leu Ala His Trp Leu Gly Glu Ala Pro Phe Asp Asp Lys His
275 280 285
Thr Pro Arg Asp His Ile Ile Thr Ala Phe Val Thr Ile Gly Glu Gly
290 295 300
Tyr His Asn Phe His His Glu Phe Pro Gln Asp Phe Arg Asn Ala Ile
305 310 315 320
Arg Trp Tyr Gln Tyr Asp Pro Thr Lys Trp Phe Ile Ala Val Ala Ala
325 330 335
Phe Leu Gly Leu Ala Ser Glu Leu Lys Thr Phe Pro Asp Asn Glu Val
340 345 350
Arg Lys Gly Gln Tyr Ser Met Lys Leu Lys Glu Leu Gln Arg Asp Phe
355 360 365
Arg Asp Val Lys Trp Pro Lys Ser Ser Asn Asp Leu Pro Ile Val Thr
370 375 380
Trp Glu Gln Phe Val Glu Glu Ala Asp Lys Lys Asn Gly Arg Asp Leu
385 390 395 400
Ile Val Val Gly Gly Phe Ile His Asp Val Thr Glu Phe Ile Asp Glu
405 410 415
His Pro Gly Gly Arg Ala Leu Ile Lys Thr Arg Leu Gly Lys Asp Ala
420 425 430
Thr Thr Ala Phe His Gly Gly Val Tyr Asp His Ser Asn Ala Ala His
435 440 445
Asn Leu Leu Ala Met Leu Arg Val Gly Val Ile Glu Gly Gly Tyr Glu
450 455 460
Val Glu His Leu Lys Lys Lys Val Gly Val Phe Arg Lys Glu Gln Gln
465 470 475 480
Ile Pro Ile Cys Gly Pro Lys Ser Leu Gly Thr Ile Ser Thr Pro Glu
485 490 495
Ser Pro Val Val Glu Val Lys Pro Ile Tyr Thr
500 505
<210> 14
<211> 1524
<212> DNA
<213> 禾柄锈菌
<400> 14
atgtccaaac cctcaccttc aactcccgcc accgcccctc atctccgtca gcgccaacgc 60
aagaacttgc cggattatga tcccgattcc gatcttagcg agtcagaggg cttgggaggc 120
ctcagatctc aagttggtaa tacttgggaa gatgatgagg aaactgcggt ggatgatgat 180
agctacgttc aacgcacctt gcgcaaggaa aagccactcc ctccaatcac ctggtgcaac 240
ttttatcgtg agatcaatat gatctccact ttggccttga ccgtcgtccc catcctggcc 300
atctacggtg ctttcactac gcccctctat cgttcaacct tggcctggtc gattctctac 360
tactacttca ccggtctcgg catcaccgcc ggttatcatc gtctttgggc ccatcgatcc 420
tacaatgcct cccttccgct ccaatatttc ctcgctctcg gtggctctgg cgccgttgag 480
ggtagtatcc gttggtgggc tcgtgggcat cgagctcatc atcgttacac tgacaccgat 540
ctagacccct actcggctca caaaggactc ctatggagtc atgtgggctg gatgattgtc 600
aaacctcgac gaaaacccgg cgtggccgat gtctctgatc tatctcgcaa tcaagtcgtc 660
agatggcagc atcgttggta tctgcctttg attttcggca tgggattctt cttccccact 720
cttgtcgctg gactcggatg gggtgattgg agaggcggat ttttctacgc tggggccgct 780
agattattgt ttgttcacca ctcgactttc tgtgtgaact ctctggctca ctggcttgga 840
gaggctccct ttgatgataa acacactcca agggatcaca tcattaccgc ctttgtcaca 900
attggagaag gttatcacaa cttccatcac gaatttcctc aggacttccg aaatgccatt 960
cgatggtacc aatacgaccc aaccaaatgg ttcatcgctg tcgctgcctt cctcggacta 1020
gcttctgagc tcaaaacctt cccggataac gaggttcgca aaggtcaata cagtatgaaa 1080
cttaaggaac tccagcgaga tttccgagac gtcaagtggc ccaagtcttc caacgacctt 1140
ccgatcgtta catgggaaca atttgtcgaa gaggctgata aaaagaacgg acgtgatttg 1200
atcgttgtcg ggggtttcat ccatgatgtg actgagttta tcgacgagca tcccggaggt 1260
cgggcactca tcaaaacccg acttggtaaa gatgccacca cggccttcca tggtggagtt 1320
tacgatcatt ccaacgccgc gcacaacttg ttggctatgc tgcgagttgg tgtgatcgag 1380
ggtggctacg aggttgaaca tctgaagaaa aaagtagggg tctttcgaaa ggaacaacag 1440
atccctatct gtggtcctaa gagcttagga accatttcta cccctgaaag tccagtggta 1500
gaagtcaagc cgatctatac ttga 1524
<210> 15
<211> 476
<212> PRT
<213> 密粘褶菌
<400> 15
Met Ala Thr Tyr Thr Pro Pro Leu Thr Pro Pro Ser Glu Pro Thr Lys
1 5 10 15
Arg Leu Lys Asn Leu Glu Pro Glu Pro Ile Asp Ile Asn Ile Pro Asp
20 25 30
Asn Tyr Val Gln His Thr Leu Lys Thr Gln Lys Glu Leu Pro Pro Ile
35 40 45
Thr Trp Ser Asn Trp Tyr Arg Glu Leu Gln Trp Ile Ser Val Leu Ala
50 55 60
Leu Thr Ile Thr Pro Ala Leu Ala Ile Tyr Gly Ala Phe Thr Thr Lys
65 70 75 80
Leu Thr Trp Gln Thr Gly Leu Phe Ser Val Phe Tyr Tyr Tyr Val Thr
85 90 95
Gly Leu Gly Ile Thr Ala Gly Tyr His Arg Leu Trp Ala His Arg Ser
100 105 110
Tyr Asn Ala Ser Lys Pro Leu Gln Tyr Phe Leu Ala Leu Ala Gly Ser
115 120 125
Gly Ala Val Glu Gly Ser Ile Lys Trp Trp Ser Arg Gly His Arg Ala
130 135 140
His His Arg Tyr Thr Asp Thr Glu Leu Asp Pro Tyr Ser Ala Gln Lys
145 150 155 160
Gly Phe Trp Trp Ser His Val Gly Trp Met Leu Phe Lys Pro Arg Arg
165 170 175
Lys Pro Gly Val Ala Asp Val Ser Asp Leu Ser Arg Asn Glu Val Val
180 185 190
Arg Trp Gln His Arg Trp Tyr Val Trp Leu Ile Leu Gly Met Gly Phe
195 200 205
Gly Leu Pro Thr Val Val Pro Gly Leu Leu Trp Gly Asp Trp Trp Gly
210 215 220
Gly Phe Phe Tyr Ala Gly Ala Leu Arg Leu Thr Phe Val His His Ser
225 230 235 240
Thr Phe Cys Val Asn Ser Leu Ala His Trp Leu Gly Glu Thr Pro Phe
245 250 255
Asp Asp Lys His Thr Pro Arg Asp His Val Ile Thr Ala Leu Val Thr
260 265 270
Ile Gly Glu Gly Tyr His Asn Phe His His Gln Phe Pro Met Asp Tyr
275 280 285
Arg Asn Ala Ile Lys Trp Tyr Gln Tyr Asp Pro Thr Lys Trp Phe Ile
290 295 300
Met Ala Cys Gln Trp Val Gly Leu Ala Ser His Leu Lys Thr Phe Pro
305 310 315 320
Asp Asn Glu Val Arg Lys Gly Gln Leu Thr Met Gln Leu Lys Arg Leu
325 330 335
Arg Glu Thr Gln Glu Lys Leu Thr Trp Ala Pro Asp Ser Asn Asp Leu
340 345 350
Pro Ile Val Ser Trp Asp Ser Phe Gln Glu Gln Ser Ala Lys Arg Pro
355 360 365
Leu Ile Leu Ile Ala Gly Phe Ile His Asp Val Ala Ser Phe Leu Asp
370 375 380
Glu His Pro Gly Gly Arg His Leu Leu Val Lys Tyr Ile Gly Lys Asp
385 390 395 400
Ala Thr Thr Ala Phe Phe Gly Gly Val Tyr Asp His Ser Asn Ala Ala
405 410 415
His Asn Leu Leu Ser Met His Arg Val Gly Ile Leu Gln His Gly Tyr
420 425 430
Arg Gln Ser Leu Asp Asp Lys Ala Ile Pro Pro Ala Gln Arg Leu Arg
435 440 445
Ile Ala Arg Tyr Asn Glu Leu Gly Ser Ser Thr Ala Val Ser Asp Ala
450 455 460
Glu Thr Leu Val Gly Glu Lys Glu Glu Lys Glu Ala
465 470 475
<210> 16
<211> 1431
<212> DNA
<213> 密粘褶菌
<400> 16
atggcgacct acacgccgcc gctgacgccg ccgtccgagc ccaccaagcg gctcaaaaat 60
ctcgagccgg agcccatcga catcaacatc cccgacaact acgtccagca cacgctcaag 120
acgcagaagg agctcccgcc tatcacctgg agcaactggt accgcgagct gcagtggatc 180
agcgtcctcg cgctcacgat cacacctgca cttgcgatct acggcgcgtt tacgaccaag 240
ctcacatggc agacgggcct cttcagcgtc ttctactact acgtgactgg cctcggtatc 300
accgccggat accaccgtct gtgggcgcac cggtcgtaca acgcctccaa gccgttgcag 360
tacttcctcg cgctcgcggg ctcgggcgcc gtcgagggct ccatcaaatg gtggtcgcgc 420
ggccaccgtg cgcaccatcg ctacaccgac accgaactcg acccgtactc ggcccagaag 480
ggcttctggt ggtcgcacgt cggctggatg ctcttcaagc cgcgccgcaa gcccggcgtc 540
gccgacgtca gcgacctcag ccgcaacgag gtcgtcaggt ggcagcaccg ctggtatgtc 600
tggctcatcc ttggcatggg cttcgggctc ccgactgttg tcccgggcct gctctggggt 660
gactggtggg gcgggttctt ctacgcgggt gctctgcgcc tgacgttcgt gcaccattcg 720
acgttctgtg tcaactccct agcgcactgg ctgggagaga cgccgttcga cgacaagcac 780
acgcccaggg accacgttat caccgcgctc gtcaccatcg gcgagggcta ccacaacttc 840
caccaccagt tccccatgga ctaccgcaac gccatcaagt ggtaccagta cgacccgaca 900
aagtggttca tcatggcctg ccagtgggtc ggcctcgcgt cacatctcaa gacgttccct 960
gataacgaag tccgcaaagg ccagctgact atgcagctca agcggctccg ggaaacgcag 1020
gagaagctca cctgggcacc ggatagcaac gacctgccca ttgtctcttg ggacagcttc 1080
caggaacagt cggcgaagcg tccgctgatc ttgattgctg gcttcatcca cgatgtcgcg 1140
tccttcttgg acgagcaccc tggcggccgg catctgctcg tgaagtacat cggcaaggac 1200
gctacgacgg cgttcttcgg cggtgtctac gatcactcca acgccgcgca taacctgctc 1260
tcgatgcacc gcgttggcat cctgcaacac ggctaccgcc agagcttgga cgataaggcc 1320
atcccgcccg cgcagcgcct gcggattgcg cggtacaacg agctcgggtc atcgacggcc 1380
gtgtcggatg cggagacgct agtgggggag aaggaggaga aggaagcgta g 1431
<210> 17
<211> 727
<212> PRT
<213> 解脂耶氏酵母
<400> 17
Met Ser Glu Thr Asp His Leu Leu Ala Ala Glu Pro Val Ala Glu Tyr
1 5 10 15
Pro Gln Tyr Thr Pro Trp Pro Asn Ser Arg Lys Ser Val Asp Thr Glu
20 25 30
Phe Ser Ala Thr Ser Trp Ile Tyr Asp Leu Val Leu Trp Ile Phe Thr
35 40 45
Ala Cys Phe Asp Ile Phe Phe Arg Glu Ile Arg Pro Arg Gly Ala Phe
50 55 60
Arg Ile Pro Arg Lys Gly Pro Val Leu Phe Val Ala Ala Pro His Ala
65 70 75 80
Asn Gln Phe Val Asp Pro Val Ile Leu Met Asn Gln Val Lys Gln Glu
85 90 95
Ala Gly Arg Arg Ile Ser Phe Leu Val Ala Glu Lys Ser Met Arg Arg
100 105 110
Ala Ala Val Gly Arg Met Ala Arg Ser Met Asn Ser Ile Pro Val Val
115 120 125
Arg Ala Gln Asp Asn Ala Lys Lys Gly Glu Gly Lys Ile Tyr Val Asp
130 135 140
Ala Glu Asp Pro Thr Lys Ile His Gly Ile Gly Thr Gln Phe Thr Lys
145 150 155 160
Gln Cys Glu Val Arg Gly Leu Val Val Cys Ser Ser Ser Val Gly Ser
165 170 175
Ile Asp Val Ala Glu Ile Val Ser Asp Thr Leu Leu Ile Ala Arg Lys
180 185 190
Glu Phe Lys Gly Pro Lys Ala Lys Glu Ala Leu Lys Glu Ser Asn Gly
195 200 205
Gly Ile Thr Tyr Lys Tyr Ala Asp Tyr Val Asn Gln Ala Thr Val Tyr
210 215 220
Arg Ser Val Phe Asp Lys Leu His His Gly Gly Cys Val Gly Ile Phe
225 230 235 240
Pro Glu Gly Gly Ser His Asp Arg Thr Glu Leu Leu Pro Leu Lys Ala
245 250 255
Gly Val Ala Ile Met Ala Leu Gly Ala Leu Ala Glu Asp Pro Ser Cys
260 265 270
Gly Val Arg Ile Val Pro Cys Gly Leu Asn Tyr Phe His Ala His Lys
275 280 285
Phe Arg Ser Arg Ala Val Val Glu Phe Gly Ser Pro Ile Ala Ile Pro
290 295 300
Pro Asp Leu Val Glu Lys Tyr Lys Ala Gly Gly Glu Ala Lys Arg Glu
305 310 315 320
Ala Val Lys Thr Val Leu Asp Ile Thr Ala Ala Gly Leu Lys Ser Val
325 330 335
Thr Val Gln Val Gln Asp Phe Asp Thr Leu Met Leu Ile Gln Ala Ile
340 345 350
Arg Arg Leu Tyr Arg Pro Pro Gly Lys Lys Ile Pro Leu Pro Met Val
355 360 365
Val Glu Leu Asn Arg Arg Leu Val Tyr Ala Tyr Asn His Tyr Lys Asp
370 375 380
Asp Pro Arg Ile Glu Glu Met Lys Gln Glu Ile Arg Lys Tyr Asn Lys
385 390 395 400
Phe Leu Gln Ala Met Gly Leu Lys Asp His Gln Val Glu Lys Ala Arg
405 410 415
Ile Ser Lys Ile Glu Ile Leu Gly Arg Leu Leu Tyr Arg Ser Ile Lys
420 425 430
Leu Val Phe Leu Ser Ile Gly Cys Leu Pro Gly Leu Leu Leu Phe Ser
435 440 445
Pro Ile Phe Ile Ile Ser Lys Ser Ile Ser Lys Thr Lys Ala Lys Glu
450 455 460
Ala Leu Lys Ala Ser Ser Val Lys Ile Lys Ala Asn Asp Val Val Ala
465 470 475 480
Thr Trp Lys Val Leu Val Ala Met Gly Leu Thr Pro Val Leu Tyr Ile
485 490 495
Leu Tyr Ser Leu Val Gly Ser Val Val Ile Arg Lys Leu Asp Leu Ile
500 505 510
Ser Trp Phe Pro Thr Ile Leu Leu Pro Gly Leu Val Leu Ser Ile Ile
515 520 525
Ile Thr Thr Ser Tyr Ala Ala Leu Ala Met Gly Glu Ala Gly Met Asp
530 535 540
Ile Phe Lys Ser Leu Arg Pro Leu Ala Leu Ala Leu Asn Pro Ser Thr
545 550 555 560
Lys Asn Ser Leu Leu Lys Leu Gln Asn Glu Arg Lys Arg Leu Val Leu
565 570 575
Lys Ser Ser Glu Leu Val Thr Ser Leu Gly Pro Glu Leu Phe Pro Asp
580 585 590
Phe Pro Glu Asn Ser Ile Leu Gln Gly Ser Asp Lys Phe Glu Asp Glu
595 600 605
Glu Asn Tyr Glu Asn Glu Lys Arg Ser His Ser Arg Ser Thr Ser Ala
610 615 620
Thr Ser Leu Ser Ala Met Ser Glu Gly Asp Gly Asp Glu Leu Val Arg
625 630 635 640
Glu Val Arg Lys Gly Ala Ser Tyr Phe Pro Val Ser Thr Ile Ser Glu
645 650 655
Asp Glu Asp Gln Ala Ile Ser Arg Val Gly Ser Glu Ala Ser Leu Ala
660 665 670
Asp Ile Pro Leu Phe Gly Met Ser Arg Ser Gln Ser Gly Ala Ser Leu
675 680 685
Ser Glu Ala Ser Thr His Gly Ser Ser Thr Gly Ala Asp Ala Glu Glu
690 695 700
Ala Lys Thr Glu Val Thr Arg Arg Ile Ala Leu Ala Met Glu Glu Lys
705 710 715 720
Arg Arg Glu Gln Asp Glu Glu
725
<210> 18
<211> 2184
<212> DNA
<213> 解脂耶氏酵母
<400> 18
atgtccgaaa ccgaccatct gctggccgcc gagcccgtgg ctgagtaccc ccagtacacg 60
ccttggccca actcccgaaa atcagtggac acggagtttt ccgcaacctc gtggatttac 120
gacttggttc tgtggatttt cacggcttgc tttgacattt ttttcagaga aatccggcca 180
cgtggtgcct tccgaatccc cagaaagggc cccgtgctgt tcgtggctgc cccccacgca 240
aaccagtttg tggaccccgt catcctcatg aaccaggtca aacaggaggc cggacgacga 300
atctccttcc ttgtggccga gaagtccatg cgacgagctg cagtcggacg aatggcccga 360
agcatgaact caattcctgt cgtgcgagct caggacaatg caaaaaaggg agagggaaag 420
atttacgtcg acgcagagga ccccacaaag atccacggaa tcggcaccca gttcacgaag 480
cagtgcgagg tgcgaggcct cgtggtctgc tcgtcctctg tcggctcaat tgacgtggct 540
gagattgtgt ccgacactct gctcattgca agaaaggaat tcaagggccc caaagccaag 600
gaggctctca aggaatccaa cggaggaatc acatacaagt acgccgacta cgtcaaccag 660
gccacagtct accgatccgt attcgacaaa ttgcaccatg gaggctgtgt gggtatcttc 720
ccagagggag gatctcatga ccgaaccgaa ctgctgcccc ttaaggccgg tgttgctatc 780
atggctctgg gggctctcgc agaggacccc tcttgtggtg tgcgaatcgt cccctgtggt 840
ctcaactact tccacgccca caagttccga tctcgggccg tggtggagtt tggctctcct 900
attgccattc ctccggatct cgtggagaag tacaaggcag gaggagaggc caagcgggag 960
gctgtcaaga ccgttctaga cattactgcc gctggtctca agtctgtgac tgttcaggtg 1020
caggatttcg acaccctgat gctgatccag gccattcgac gactctaccg acctcccgga 1080
aagaagattc ctctgcccat ggttgtagag ctcaaccgtc gacttgtata cgcctacaac 1140
cactacaagg acgatccccg tatcgaggag atgaagcagg agattcgaaa gtacaacaag 1200
ttcctgcagg ccatgggtct caaggaccat caggtagaga aggcccgaat ctccaagatt 1260
gagattctgg gccggcttct gtaccggtcc atcaagcttg tgttcttgtc cattggctgt 1320
ctccccggtc tgcttttgtt ttctcccatc ttcatcattt ctaagtccat ttccaaaacc 1380
aaggccaagg aggctctcaa ggcctccagt gtcaaaatca aggctaacga tgtggttgcc 1440
acttggaagg tgctggttgc aatgggtctg accccagttc tttacattct ctattcactg 1500
gttggatctg tggtgattcg aaagctcgat ctcatctcct ggttccccac aattcttctt 1560
cccggcctcg ttttaagcat catcatcaca acctcatacg ccgccctggc tatgggagag 1620
gccggtatgg acattttcaa gtctcttcga ccacttgcat tggctctcaa cccttccacc 1680
aaaaactctc tgctcaagct gcaaaatgaa cgaaagcgac ttgtgctcaa gtcttccgag 1740
ctcgttacct ctttgggccc tgagctgttc cccgacttcc ccgagaactc cattctgcag 1800
ggaagcgata agtttgagga cgaggagaac tacgaaaacg agaagcgatc gcattccaga 1860
tccacttctg ccacttctct atctgccatg agcgagggag acggtgatga gcttgttcgg 1920
gaggtccgaa agggtgctag ctacttccct gtgagtacca tttctgagga cgaagaccaa 1980
gccatctcgc gagtgggctc tgaggcatct cttgctgaca ttcctctgtt tggtatgtcc 2040
cgatcacaat ctggagcttc tctttcggaa gcctccacac acggctcttc tactggagct 2100
gatgccgagg aggctaagac ggaggtgact cgcagaattg cattggcgat ggaggaaaaa 2160
cgacgagagc aggatgagga ataa 2184
<210> 19
<211> 514
<212> PRT
<213> 解脂耶氏酵母
<400> 19
Met Thr Ile Asp Ser Gln Tyr Tyr Lys Ser Arg Asp Lys Asn Asp Thr
1 5 10 15
Ala Pro Lys Ile Ala Gly Ile Arg Tyr Ala Pro Leu Ser Thr Pro Leu
20 25 30
Leu Asn Arg Cys Glu Thr Phe Ser Leu Val Trp His Ile Phe Ser Ile
35 40 45
Pro Thr Phe Leu Thr Ile Phe Met Leu Cys Cys Ala Ile Pro Leu Leu
50 55 60
Trp Pro Phe Val Ile Ala Tyr Val Val Tyr Ala Val Lys Asp Asp Ser
65 70 75 80
Pro Ser Asn Gly Gly Val Val Lys Arg Tyr Ser Pro Ile Ser Arg Asn
85 90 95
Phe Phe Ile Trp Lys Leu Phe Gly Arg Tyr Phe Pro Ile Thr Leu His
100 105 110
Lys Thr Val Asp Leu Glu Pro Thr His Thr Tyr Tyr Pro Leu Asp Val
115 120 125
Gln Glu Tyr His Leu Ile Ala Glu Arg Tyr Trp Pro Gln Asn Lys Tyr
130 135 140
Leu Arg Ala Ile Ile Thr Thr Ile Glu Tyr Phe Leu Pro Ala Phe Met
145 150 155 160
Lys Arg Ser Leu Ser Ile Asn Glu Gln Glu Gln Pro Ala Glu Arg Asp
165 170 175
Pro Leu Leu Ser Pro Val Ser Pro Ser Ser Pro Gly Ser Gln Pro Asp
180 185 190
Lys Trp Ile Asn His Asp Ser Arg Tyr Ser Arg Gly Glu Ser Ser Gly
195 200 205
Ser Asn Gly His Ala Ser Gly Ser Glu Leu Asn Gly Asn Gly Asn Asn
210 215 220
Gly Thr Thr Asn Arg Arg Pro Leu Ser Ser Ala Ser Ala Gly Ser Thr
225 230 235 240
Ala Ser Asp Ser Thr Leu Leu Asn Gly Ser Leu Asn Ser Tyr Ala Asn
245 250 255
Gln Ile Ile Gly Glu Asn Asp Pro Gln Leu Ser Pro Thr Lys Leu Lys
260 265 270
Pro Thr Gly Arg Lys Tyr Ile Phe Gly Tyr His Pro His Gly Ile Ile
275 280 285
Gly Met Gly Ala Phe Gly Gly Ile Ala Thr Glu Gly Ala Gly Trp Ser
290 295 300
Lys Leu Phe Pro Gly Ile Pro Val Ser Leu Met Thr Leu Thr Asn Asn
305 310 315 320
Phe Arg Val Pro Leu Tyr Arg Glu Tyr Leu Met Ser Leu Gly Val Ala
325 330 335
Ser Val Ser Lys Lys Ser Cys Lys Ala Leu Leu Lys Arg Asn Gln Ser
340 345 350
Ile Cys Ile Val Val Gly Gly Ala Gln Glu Ser Leu Leu Ala Arg Pro
355 360 365
Gly Val Met Asp Leu Val Leu Leu Lys Arg Lys Gly Phe Val Arg Leu
370 375 380
Gly Met Glu Val Gly Asn Val Ala Leu Val Pro Ile Met Ala Phe Gly
385 390 395 400
Glu Asn Asp Leu Tyr Asp Gln Val Ser Asn Asp Lys Ser Ser Lys Leu
405 410 415
Tyr Arg Phe Gln Gln Phe Val Lys Asn Phe Leu Gly Phe Thr Leu Pro
420 425 430
Leu Met His Ala Arg Gly Val Phe Asn Tyr Asp Val Gly Leu Val Pro
435 440 445
Tyr Arg Arg Pro Val Asn Ile Val Val Gly Ser Pro Ile Asp Leu Pro
450 455 460
Tyr Leu Pro His Pro Thr Asp Glu Glu Val Ser Glu Tyr His Asp Arg
465 470 475 480
Tyr Ile Ala Glu Leu Gln Arg Ile Tyr Asn Glu His Lys Asp Glu Tyr
485 490 495
Phe Ile Asp Trp Thr Glu Glu Gly Lys Gly Ala Pro Glu Phe Arg Met
500 505 510
Ile Glu
<210> 20
<211> 1545
<212> DNA
<213> 解脂耶氏酵母
<400> 20
atgactatcg actcacaata ctacaagtcg cgagacaaaa acgacacggc acccaaaatc 60
gcgggaatcc gatatgcccc gctatcgaca ccattactca accgatgtga gaccttctct 120
ctggtctggc acattttcag cattcccact ttcctcacaa ttttcatgct atgctgcgca 180
attccactgc tctggccatt tgtgattgcg tatgtagtgt acgctgttaa agacgactcc 240
ccgtccaacg gaggagtggt caagcgatac tcgcctattt caagaaactt cttcatctgg 300
aagctctttg gccgctactt ccccataact ctgcacaaga cggtggatct ggagcccacg 360
cacacatact accctctgga cgtccaggag tatcacctga ttgctgagag atactggccg 420
cagaacaagt acctccgagc aatcatctcc accatcgagt actttctgcc cgccttcatg 480
aaacggtctc tttctatcaa cgagcaggag cagcctgccg agcgagatcc tctcctgtct 540
cccgtttctc ccagctctcc gggttctcaa cctgacaagt ggattaacca cgacagcaga 600
tatagccgtg gagaatcatc tggctccaac ggccacgcct cgggctccga acttaacggc 660
aacggcaaca atggcaccac taaccgacga cctttgtcgt ccgcctctgc tggctccact 720
gcatctgatt ccacgcttct taacgggtcc ctcaactcct acgccaacca gatcattggc 780
gaaaacgacc cacagctgtc gcccacaaaa ctcaagccca ctggcagaaa atacatcttc 840
ggctaccacc cccacggcat tatcggcatg ggagcctttg gtggaattgc caccgaggga 900
gctggatggt ccaagctctt tccgggcatc cctgtttctc ttatgactct caccaacaac 960
ttccgagtgc ctctctacag agagtacctc atgagtctgg gagtcgcttc tgtctccaag 1020
aagtcctgca aggccctcct caagcgaaac cagtctatct gcattgtcgt tggtggagca 1080
caggaaagtc ttctggccag acccggtgtc atggacctgg tgctactcaa gcgaaagggt 1140
tttgttcgac ttggtatgga ggtcggaaat gtcgcccttg ttcccatcat ggcctttggt 1200
gagaacgacc tctatgacca ggttagcaac gacaagtcgt ccaagctgta ccgattccag 1260
cagtttgtca agaacttcct tggattcacc cttcctttga tgcatgcccg aggcgtcttc 1320
aactacgatg tcggtcttgt cccctacagg cgacccgtca acattgtggt tggttccccc 1380
attgacttgc cttatctccc acaccccacc gacgaagaag tgtccgaata ccacgaccga 1440
tacatcgccg agctgcagcg aatctacaac gagcacaagg atgaatattt catcgattgg 1500
accgaggagg gcaaaggagc cccagagttc cgaatgattg agtaa 1545
<210> 21
<211> 348
<212> PRT
<213> 圆红冬孢酵母
<400> 21
Met Gly Gln Gln Ala Thr Pro Glu Glu Leu Tyr Thr Arg Ser Glu Ile
1 5 10 15
Ser Lys Ile Lys Phe Ala Pro Phe Gly Val Pro Arg Ser Arg Arg Leu
20 25 30
Gln Thr Phe Ser Val Phe Ala Trp Thr Thr Ala Leu Pro Ile Leu Leu
35 40 45
Gly Val Phe Phe Leu Leu Cys Ser Phe Pro Pro Leu Trp Pro Ala Val
50 55 60
Ile Ala Tyr Leu Thr Trp Val Phe Phe Ile Asp Gln Ala Pro Ile His
65 70 75 80
Gly Gly Arg Ala Gln Ser Trp Leu Arg Lys Ser Arg Ile Trp Val Trp
85 90 95
Phe Ala Gly Tyr Tyr Pro Val Ser Leu Ile Lys Ser Ala Asp Leu Pro
100 105 110
Pro Asp Arg Lys Tyr Val Phe Gly Tyr His Pro His Gly Val Ile Gly
115 120 125
Met Gly Ala Ile Ala Asn Phe Ala Thr Asp Ala Thr Gly Phe Ser Thr
130 135 140
Leu Phe Pro Gly Leu Asn Pro His Leu Leu Thr Leu Gln Ser Asn Phe
145 150 155 160
Lys Leu Pro Leu Tyr Arg Glu Leu Leu Leu Ala Leu Gly Ile Cys Ser
165 170 175
Val Ser Met Lys Ser Cys Gln Asn Ile Leu Arg Gln Gly Pro Gly Ser
180 185 190
Ala Leu Thr Ile Val Val Gly Gly Ala Ala Glu Ser Leu Ser Ala His
195 200 205
Pro Gly Thr Ala Asp Leu Thr Leu Lys Arg Arg Lys Gly Phe Ile Lys
210 215 220
Leu Ala Ile Arg Gln Gly Ala Asp Leu Val Pro Val Phe Ser Phe Gly
225 230 235 240
Glu Asn Asp Ile Phe Gly Gln Leu Arg Asn Glu Arg Gly Thr Arg Leu
245 250 255
Tyr Lys Leu Gln Lys Arg Phe Gln Gly Val Phe Gly Phe Thr Leu Pro
260 265 270
Leu Phe Tyr Gly Arg Gly Leu Phe Asn Tyr Asn Val Gly Leu Met Pro
275 280 285
Tyr Arg His Pro Ile Val Ser Val Val Gly Arg Pro Ile Ser Val Glu
290 295 300
Gln Lys Asp His Pro Thr Thr Ala Asp Leu Glu Glu Val Gln Ala Arg
305 310 315 320
Tyr Ile Ala Glu Leu Lys Arg Ile Trp Glu Glu Tyr Lys Asp Ala Tyr
325 330 335
Ala Lys Ser Arg Thr Arg Glu Leu Asn Ile Ile Ala
340 345
<210> 22
<211> 1047
<212> DNA
<213> 圆红冬孢酵母
<400> 22
atgggccagc aggcgacgcc cgaggagcta tacacacgct cagagatctc caagatcaag 60
ttcgcaccct ttggcgtccc gcggtcgcgc cggctgcaga ccttctccgt ctttgcctgg 120
acgacggcac tgcccatcct actcggcgtc ttcttcctcc tctgctcgtt cccaccgctc 180
tggccggctg tcattgccta cctcacctgg gtctttttca ttgaccaggc gccgattcac 240
ggtggacggg cgcagtcttg gctgcggaag agtcggatat gggtctggtt tgcaggatac 300
tatcccgtca gcttgatcaa gagcgccgac ttgccgcctg accggaagta cgtctttggc 360
taccacccgc acggcgtcat aggcatgggc gccatcgcca acttcgcgac cgacgcaacc 420
ggcttctcga cactcttccc cggcttgaac cctcacctcc tcaccctcca aagcaacttc 480
aagctcccgc tctaccgcga gttgctgctc gctctcggca tatgctccgt ctcgatgaag 540
agctgtcaga acattctgcg acaaggtcct ggctcggctc tcactatcgt cgtcggtggc 600
gccgccgaga gcttgagtgc gcatcccgga accgccgatc ttacgctcaa gcgacgaaaa 660
ggcttcatca aactcgcgat ccggcaaggc gccgaccttg tgcccgtctt ttcgttcggc 720
gagaacgaca tctttggcca gctgcgaaac gagcgaggaa cgcggctgta caagttgcag 780
aagcgtttcc aaggcgtgtt tggcttcacc ctccctctct tctacggccg gggactcttc 840
aactacaacg tcggattgat gccgtatcgc catccgatcg tctctgtcgt cggtcgacca 900
atctcggtag agcagaagga ccacccgacc acggcggacc tcgaagaagt tcaggcgcgg 960
tatatcgcag aactcaagcg gatctgggaa gaatacaagg acgcctacgc caaaagtcgc 1020
acgcgggagc tcaatattat cgcctga 1047
<210> 23
<211> 410
<212> PRT
<213> 斯达氏油脂酵母
<400> 23
Met Ser Glu Lys Ala Glu Ile Glu Val Pro Pro Gln Lys Ser Thr Phe
1 5 10 15
Pro Arg Ser Val His Phe Ala Pro Leu His Ile Pro Leu Glu Arg Arg
20 25 30
Leu Gln Thr Leu Ala Val Leu Phe His Thr Val Ala Leu Pro Tyr Cys
35 40 45
Ile Gly Leu Phe Phe Leu Met Leu Ala Phe Pro Pro Phe Trp Pro Leu
50 55 60
Leu Val Met Tyr Val Ile Tyr Ala Tyr Gly Phe Asp His Ser Ser Ser
65 70 75 80
Asn Gly Glu Ile Ser Arg Arg Arg Ser Pro Leu Phe Arg Arg Leu Pro
85 90 95
Leu Phe Arg Leu Tyr Cys Asp Tyr Phe Pro Ile His Ile His Arg Glu
100 105 110
Val Pro Leu Glu Pro Thr Phe Pro Gly Arg Leu Arg Glu Pro Ser Gly
115 120 125
Leu Val Glu Arg Trp Ile Ala Lys Met Phe Gly Val Gln Asp Ala Val
130 135 140
Val Glu Gly Asn Glu Ser Asp Val Lys Ala Thr Ala Asn Gly Asn Gly
145 150 155 160
Thr Thr Lys Glu Ile Gly Pro Thr Tyr Val Phe Gly Tyr His Pro His
165 170 175
Gly Ile Val Ser Leu Gly Ala Phe Gly Ala Ile Gly Thr Glu Gly Ala
180 185 190
Gly Trp Glu Lys Leu Phe Pro Gly Ile Pro Val Ser Leu Leu Thr Leu
195 200 205
Glu Thr Asn Phe Ser Leu Pro Phe Tyr Arg Glu Tyr Leu Leu Ser Leu
210 215 220
Gly Ile Ala Ser Val Ser Arg Arg Ser Cys Thr Asn Leu Leu Lys His
225 230 235 240
Asp Gln Ser Ile Cys Ile Val Ile Gly Gly Ala Gln Glu Ser Leu Leu
245 250 255
Ala Glu Pro Gly Thr Leu Asp Leu Ile Leu Val Lys Arg Arg Gly Phe
260 265 270
Val Lys Leu Ala Met Ser Thr Ala Arg Val Ser Asp Gln Pro Ile Cys
275 280 285
Leu Val Pro Ile Leu Ser Phe Gly Glu Asn Asp Val Tyr Asp Gln Val
290 295 300
Arg Gly Asp Arg Ser Ser Lys Leu Tyr Lys Ile Gln Thr Phe Ile Lys
305 310 315 320
Lys Ala Ala Gly Phe Thr Leu Pro Leu Met Tyr Ala Arg Gly Ile Phe
325 330 335
Asn Tyr Asp Phe Gly Leu Met Pro Tyr Arg Arg Gln Met Thr Leu Val
340 345 350
Val Gly Lys Pro Ile Ala Val Pro Tyr Val Ala Gln Pro Thr Glu Ala
355 360 365
Glu Ile Glu Val Tyr His Lys Gln Tyr Met Asp Glu Leu Arg Arg Leu
370 375 380
Trp Asp Thr Tyr Lys Asp Asp Tyr Phe Val Asp His Lys Gly Lys Gly
385 390 395 400
Val Lys Asn Ser Glu Met Arg Phe Val Glu
405 410
<210> 24
<211> 1233
<212> DNA
<213> 斯达氏油脂酵母
<400> 24
atgagtgaga aggcagagat cgaggttccg ccgcaaaaat cgacattccc tcgcagtgtg 60
cacttcgctc cacttcatat tccactggag agacgcctac agactttggc agtcttattc 120
cacactgtcg cgctaccata ctgcatcggt ctgttctttc tcatgctcgc gttccctcct 180
ttttggccat tattggtaat gtatgtcata tacgcatacg ggttcgacca ctcgagctcg 240
aacggagaga tctcccgccg gcgatcgccg ctgtttcgaa gactcccgtt gttcaggctg 300
tattgtgatt acttccccat ccacattcac cgggaggttc cgctcgagcc gacgtttcct 360
ggtcgccttc gcgaaccgag tggccttgtc gagcggtgga ttgcgaagat gttcggcgtg 420
caggacgctg ttgtcgaggg aaatgaatct gacgttaagg ccacggccaa cggcaatggg 480
acgacgaaag aaatcggacc gacgtatgtt ttcggctatc atccgcatgg aattgttagc 540
ttgggtgcgt ttggtgctat tggtacggaa ggcgctggat gggagaagct ctttcctggg 600
atcccggtgt cactgctgac tctcgaaaca aatttcagcc ttccatttta cagagagtat 660
ttgctgtcac ttgggattgc ttcagtatct cgacggtctt gtaccaatct cctcaaacac 720
gaccaatcca tctgcatcgt tatcggcggc gcccaagagt cgctcttagc ggaaccaggc 780
actctagatc tgatcctcgt taaacgtcgc ggttttgtca aacttgcaat gtcaacggcg 840
cgggtatctg accaaccgat ttgtcttgtt ccgatcctca gtttcggcga gaacgacgtg 900
tacgaccaag tccgcgggga ccgatcgtcg aagttgtata agatccagac ttttatcaag 960
aaagcggccg ggtttacgct accattgatg tatgcgcgcg gtatatttaa ttacgacttt 1020
gggctgatgc cgtaccgcag gcaaatgacg ctcgtggtcg gcaagccgat tgcagtgccg 1080
tacgtggccc agcctacgga ggctgaaatc gaagtgtatc acaagcagta catggatgaa 1140
ttgaggaggt tatgggacac gtataaggac gactattttg tagaccacaa gggcaagggg 1200
gtcaagaatt ccgagatgcg ttttgtggag taa 1233
<210> 25
<211> 380
<212> PRT
<213> 土曲霉
<400> 25
Met Pro Arg Asn Thr His Pro Pro Ala Asn Asn Ala Gly Pro Asn Ala
1 5 10 15
Ser His Lys Lys Asp Arg Lys Arg Gln Gly Arg Leu Phe Gln His Thr
20 25 30
Val Pro Asn Lys Tyr Ser Arg Ile Arg Trp Ala Pro Leu Asn Ile Gly
35 40 45
Leu Glu Arg Arg Leu Gln Thr Leu Val Val Leu Cys His Thr Leu Thr
50 55 60
Ile Ala Leu Phe Leu Ala Phe Phe Phe Phe Thr Cys Ala Ile Pro Leu
65 70 75 80
Thr Trp Pro Leu Leu Phe Pro Tyr Leu Val Tyr Ile Thr Leu Phe Ser
85 90 95
Thr Ala Pro Thr Ser Gly Thr Leu Lys Gly Arg Ser Asp Phe Leu Arg
100 105 110
Ser Leu Pro Ile Trp Lys Leu Tyr Thr Ala Tyr Phe Pro Ala Lys Leu
115 120 125
His Arg Ser Glu Pro Leu Leu Pro Thr Arg Lys Tyr Ile Phe Gly Tyr
130 135 140
His Pro His Gly Ile Ile Ser His Gly Ala Phe Ala Ala Phe Ala Thr
145 150 155 160
Asp Ala Leu Gly Phe Ser Lys Leu Phe Pro Gly Ile Thr Asn Thr Leu
165 170 175
Leu Thr Leu Asp Ser Asn Phe Arg Ile Pro Phe Tyr Arg Glu Tyr Ala
180 185 190
Met Ala Met Gly Val Ala Ser Val Ser Arg Glu Ser Cys Glu Asn Leu
195 200 205
Leu Thr Lys Gly Gly Ala Asp Gly Glu Gly Met Gly Arg Ala Ile Thr
210 215 220
Ile Val Val Gly Gly Ala Arg Glu Ser Leu Asp Ala Leu Pro His Thr
225 230 235 240
Met Arg Leu Val Leu Lys Arg Arg Lys Gly Phe Ile Lys Leu Ala Ile
245 250 255
Arg Thr Gly Ala Asp Leu Val Pro Val Leu Ala Phe Gly Glu Asn Asp
260 265 270
Leu Tyr Glu Gln Val Arg Ser Asp Gln His Pro Leu Ile Tyr Lys Val
275 280 285
Gln Met Leu Val Lys Arg Phe Leu Gly Phe Thr Val Pro Leu Phe His
290 295 300
Ala Arg Gly Ile Phe Asn Tyr Asp Val Gly Leu Met Pro Tyr Arg Arg
305 310 315 320
Pro Leu Asn Ile Val Val Gly Arg Pro Ile Gln Val Val Arg Gln Gln
325 330 335
Asp Arg Asp Lys Ile Asp Asp Glu Tyr Ile Asp Arg Leu His Ala Glu
340 345 350
Tyr Val Arg Glu Leu Glu Ser Leu Trp Asp Gln Trp Lys Asp Val Tyr
355 360 365
Ala Lys Asp Arg Ile Ser Glu Leu Glu Ile Val Ala
370 375 380
<210> 26
<211> 1143
<212> DNA
<213> 土曲霉
<400> 26
atgccccgaa acacccaccc ccccgccaac aacgccggac ctaacgcctc tcacaagaag 60
gaccgaaagc gacagggacg actctttcag cacaccgttc ctaacaagta ctctcgaatc 120
cgatgggccc ccctcaacat tggcctggag cgacgactgc agaccctcgt cgtgctgtgc 180
cataccctca ctatcgccct gttcctcgct ttctttttct ttacttgtgc cattcccctg 240
acctggcctc tgctcttccc ctacctcgtg tacatcaccc tgttttcgac cgctcctact 300
tccggtaccc tgaagggacg atctgacttc ctccgatcgc tgcctatttg gaagctctac 360
actgcctact ttcccgctaa gctgcaccga tccgagcctc tgctccctac ccgaaagtac 420
atcttcggct accaccccca tggtatcatt tcccatggag ccttcgccgc ttttgccact 480
gacgctctcg gcttctctaa gctgtttcct ggtatcacca acactctgct caccctggat 540
tcgaacttcc gaattccctt ttaccgagag tacgccatgg ctatgggagt ggcttccgtt 600
tctcgagagt cgtgcgagaa cctgctcact aagggaggtg ctgacggaga gggaatgggc 660
cgagctatca ccattgttgt cggaggcgcc cgagagtccc tcgatgctct gcctcacact 720
atgcgactgg tcctcaagcg acgaaagggt ttcatcaagc tggccattcg aaccggagct 780
gacctcgttc ccgtcctggc cttcggcgag aacgacctct acgagcaggt gcgatctgat 840
cagcaccctc tgatctacaa ggtccagatg ctcgtgaagc gattcctggg ttttaccgtg 900
cccctgttcc atgctcgagg aatttttaac tacgacgttg gcctcatgcc ttaccgacga 960
cccctgaaca tcgtggttgg tcgacccatt caggtcgtgc gacagcagga ccgagataag 1020
atcgacgatg agtacattga ccgactccac gccgagtacg tccgagagct cgagtccctg 1080
tgggaccagt ggaaggatgt ttacgccaag gaccgaatct ctgagctgga gattgtcgct 1140
taa 1143
<210> 27
<211> 437
<212> PRT
<213> 紫色麦角菌
<400> 27
Met Ala Ala Val Gln Val Ala Arg Pro Val Pro Pro His His His Asp
1 5 10 15
Gly Ala Gly Arg Glu His Lys Gly Glu Arg Ala His Ser Pro Glu Arg
20 25 30
Gly Glu Lys Thr Val His Asn Gly Tyr Gly Leu Ala Glu Thr His Glu
35 40 45
Pro Leu Glu Leu Asn Gly Ser Ala Val Gln Asp Gly Lys His Asp Ser
50 55 60
Asp Glu Thr Ile Thr Asn Gly Asp Tyr Ser Pro Tyr Pro Glu Leu Asp
65 70 75 80
Cys Gly Lys Glu Arg Ala Ala His Glu Lys Glu Ala Trp Thr Ala Gly
85 90 95
Gly Val Arg Phe Ala Pro Leu Arg Val Pro Phe Lys Arg Arg Met Gln
100 105 110
Thr Ala Ala Val Leu Phe His Cys Met Ser Ile Ile Leu Ile Ser Ser
115 120 125
Cys Phe Trp Phe Ser Leu Ala Asn Pro Ile Thr Trp Pro Ile Leu Val
130 135 140
Pro Tyr Leu Val His Leu Ser Leu Ser Asn Ala Ser Thr Asp Gly Lys
145 150 155 160
Leu Ser Tyr Arg Ser Glu Trp Leu Arg Ser Leu Pro Leu Trp Arg Leu
165 170 175
Phe Ala Gly Tyr Phe Pro Ala Lys Leu His Lys Thr Phe Asp Leu Pro
180 185 190
Pro Asn Arg Lys Tyr Ile Phe Gly Tyr His Pro His Gly Ile Ile Ser
195 200 205
His Gly Ala Trp Cys Ala Phe Ala Thr Asn Ala Leu Gly Phe Val Glu
210 215 220
Lys Phe Pro Gly Ile Thr Asn Ser Leu Leu Thr Leu Asp Ser Asn Phe
225 230 235 240
Arg Val Pro Phe Tyr Arg Asp Trp Ile Leu Ala Met Gly Ile Arg Ser
245 250 255
Val Ser Arg Glu Ser Ile Arg Asn Ile Leu Ser Lys Gly Gly Pro Asp
260 265 270
Ser Asn Gly Gln Gly Arg Ala Val Thr Ile Val Ile Gly Gly Ala Arg
275 280 285
Glu Ser Leu Glu Ala Gln Pro Gly Thr Leu Arg Leu Ile Leu Gln Gly
290 295 300
Arg Lys Gly Phe Ile Lys Val Ala Leu Arg Ala Gly Ala Asp Leu Val
305 310 315 320
Pro Val Ile Gly Phe Gly Glu Asn Asp Leu Tyr Asp Gln Leu Ser Pro
325 330 335
Lys Thr His Pro Leu Val His Lys Ile Gln Met Phe Phe Leu Lys Val
340 345 350
Phe Lys Phe Thr Ile Pro Ala Leu His Gly Arg Gly Leu Leu Asn Tyr
355 360 365
Asp Val Gly Leu Leu Pro Tyr Arg Arg Ala Val Asn Ile Val Val Gly
370 375 380
Arg Pro Ile Gln Ile Asp Glu Thr Tyr Gly Glu Gln Pro Pro Gln Glu
385 390 395 400
Val Ile Asp Arg Tyr His Glu Leu Tyr Val Gln Glu Val Glu Arg Leu
405 410 415
Tyr Ala Ala Tyr Lys Glu Gln Phe Ser Asn Gly Lys Lys Thr Pro Glu
420 425 430
Leu Gln Ile Leu Ser
435
<210> 28
<211> 1314
<212> DNA
<213> 紫色麦角菌
<400> 28
atggctgctg ttcaggttgc ccgacccgtt cccccccacc accacgatgg cgctggccga 60
gagcacaagg gagagcgagc ccattcccct gagcgaggag agaagaccgt ccacaacggc 120
tacggtctgg ccgagactca tgagcccctg gagctcaacg gttctgctgt gcaggacgga 180
aagcacgact cggatgagac catcactaac ggtgactact ctccctaccc tgagctcgat 240
tgcggaaagg agcgagccgc tcatgagaag gaggcttgga ccgctggagg tgtgcgattc 300
gctcctctgc gagttccttt taagcgacga atgcagactg ccgctgtcct cttccactgc 360
atgtccatca ttctgatttc ctcttgtttc tggttttctc tcgccaaccc catcacctgg 420
cctattctcg ttccctacct ggtccacctg tcgctctcca acgcttctac tgacggcaag 480
ctctcctacc gatctgagtg gctgcgatcc ctgcctctct ggcgactgtt cgccggttac 540
tttcccgcta agctccacaa gaccttcgat ctgcccccta accgaaagta catctttggt 600
taccaccccc atggaatcat ttcccatggc gcctggtgtg ccttcgctac caacgctctg 660
ggcttcgttg agaagtttcc tggtattacc aactcgctgc tcactctcga ctccaacttc 720
cgagtgccct tttaccgaga ttggatcctg gccatgggca ttcgatctgt ttcgcgagag 780
tctatccgaa acattctctc gaagggagga cctgactcca acggacaggg ccgagctgtg 840
accatcgtta ttggtggagc ccgagagtct ctggaggctc agcccggaac tctgcgactc 900
attctgcagg gccgaaaggg cttcattaag gtggctctcc gagctggagc tgacctggtt 960
cccgtcatcg gtttcggaga gaacgacctc tacgatcagc tgtcccctaa gacccacccc 1020
ctcgttcata agatccagat gttctttctg aaggtcttca agtttactat tcctgctctg 1080
cacggacgag gtctgctcaa ctacgacgtc ggtctgctcc cttaccgacg agctgtgaac 1140
atcgtcgtgg gacgacccat ccagattgac gagacctacg gcgagcagcc ccctcaggag 1200
gtcatcgatc gataccacga gctctacgtc caggaggtgg agcgactgta cgccgcttac 1260
aaggagcagt tctcgaacgg aaagaagacc cccgagctcc agatcctgtc ctaa 1314
<210> 29
<211> 351
<212> PRT
<213> 裂殖壶菌
<400> 29
Met Leu Ala Trp Met Pro Val Leu Ile Ala Leu Pro Arg Arg Lys Gln
1 5 10 15
Thr Ala Val Val Leu Leu Phe Val Met Leu Leu Pro Met Ile Met Val
20 25 30
Val Tyr Ser Trp Thr Leu Ile Leu Leu Ile Phe Pro Leu Thr Thr Leu
35 40 45
Pro Thr Leu Ser Tyr Leu Ile Trp Ile Met Tyr Ile Asp Lys Ser His
50 55 60
Glu Thr Gly Lys Arg Lys Pro Phe Met Arg Tyr Trp Lys Met Trp Arg
65 70 75 80
His Phe Ala Asn Tyr Phe Pro Leu Arg Leu Ile Arg Thr Thr Pro Leu
85 90 95
Asp Pro Arg Arg Lys Tyr Val Phe Cys Tyr His Pro His Gly Ile Ile
100 105 110
Ser Leu Gly Ala Phe Gly Asn Phe Ala Thr Asp Ser Thr Gly Phe Ser
115 120 125
Arg Lys Phe Pro Gly Ile Asp Leu Arg Leu Leu Thr Leu Gln Ile Asn
130 135 140
Phe Tyr Cys Pro Ile Ile Arg Glu Leu Leu Leu Tyr Met Gly Leu Cys
145 150 155 160
Ser Ala Ala Lys Lys Ser Cys Asn Gln Ile Leu Gln Arg Gly Pro Gly
165 170 175
Ser Ala Ile Met Leu Val Val Gly Gly Ala Ala Glu Ser Leu Asp Ser
180 185 190
Gln Pro Gly Thr Tyr Arg Leu Thr Leu Gly Arg Lys Gly Phe Val Arg
195 200 205
Val Ala Leu Asp Asn Gly Ala Asp Leu Val Pro Val Leu Gly Phe Gly
210 215 220
Glu Asn Asp Val Phe Asp Thr Val Tyr Leu Pro Pro Asn Ser Trp Ala
225 230 235 240
Arg Asn Val Gln Glu Phe Val Arg Lys Lys Leu Gly Phe Ala Thr Pro
245 250 255
Ile Phe Ser Gly Arg Gly Ile Phe Gln Tyr Asn Met Gly Leu Met Pro
260 265 270
His Arg Lys Pro Ile Ile Val Val Val Gly Lys Pro Ile Lys Ile Pro
275 280 285
Lys Ile Pro Asp Glu Leu Lys Gly Arg Ala Leu Ser Thr Thr Ala Glu
290 295 300
Gly Val Ala Leu Val Asp Lys Tyr His Glu Lys Tyr Val Arg Ala Leu
305 310 315 320
Arg Glu Leu Trp Asn Leu Tyr Lys Glu Glu Tyr Ala Thr Glu Pro Lys
325 330 335
Ala Ala Tyr Leu Glu Pro Asn Ser Ile Arg Lys Asn Gln Asn Val
340 345 350
<210> 30
<211> 1056
<212> DNA
<213> 裂殖壶菌
<400> 30
atgctcgcct ggatgcctgt cctcattgcc ctcccccgac gaaagcagac cgctgttgtt 60
ctcctgtttg tgatgctcct ccctatgatc atggtcgtgt actcctggac cctgatcctg 120
ctcattttcc ccctcaccac tctgcctact ctctcctacc tgatctggat tatgtacatt 180
gacaagtctc acgagaccgg aaagcgaaag ccctttatgc gatactggaa gatgtggcga 240
catttcgcca actactttcc tctccgactg atccgaacca ctcccctgga ccctcgacga 300
aagtacgtgt tctgctacca cccccatggc atcatttccc tcggagcctt cggcaacttt 360
gctaccgact cgactggctt ctcccgaaag tttcccggta tcgatctgcg actgctcacc 420
ctccagatta acttctactg tcctatcatt cgagagctgc tcctgtacat gggtctgtgc 480
tctgccgcta agaagtcgtg taaccagatc ctccagcgag gacccggctc tgctattatg 540
ctggttgtcg gcggtgccgc tgagtccctc gactctcagc ctggcaccta ccgactcact 600
ctgggtcgaa agggattcgt gcgagttgcc ctggacaacg gtgctgatct ggtccccgtg 660
ctcggtttcg gagagaacga cgtgtttgat accgtttacc tgccccctaa ctcgtgggcc 720
cgaaacgtcc aggagttcgt gcgaaagaag ctcggattcg ctacccccat cttttccggc 780
cgaggtattt ttcagtacaa catgggtctg atgccccacc gaaagcctat cattgtggtt 840
gtcggaaagc ccatcaagat tcccaagatc cctgacgagc tgaagggacg agccctctct 900
accactgccg agggcgttgc tctggtcgat aagtaccatg agaagtacgt tcgagccctc 960
cgagagctgt ggaacctcta caaggaggag tacgctaccg agcccaaggc cgcttacctc 1020
gagcctaact cgattcgaaa gaaccagaac gtctaa 1056
<210> 31
<211> 697
<212> PRT
<213> 圆红冬孢酵母
<400> 31
Met Thr Glu Arg Ser Leu Pro Val Thr Leu Pro Leu Pro Arg Asn Phe
1 5 10 15
Ala Leu Thr Pro His Gln Met Ala Ser Pro Asp Pro Pro Leu Pro Gly
20 25 30
Pro Ala Asn Leu Val Asp Asp Ala Leu Arg His Pro Asp Ser Ala Pro
35 40 45
Pro Ile Ser Pro Asp Ser Ala Pro Pro Ser Thr Ala Thr Arg Pro Ser
50 55 60
Ala Leu Ser Arg Gly Glu Leu Ser Thr Ala Ser Ser Tyr Ala Ser Glu
65 70 75 80
Val Ser Thr Arg Glu Gly Thr Pro Asp Leu Ala Asn Gly Gln Gly Val
85 90 95
Thr Thr Thr Ile Thr Thr Val Thr Gly Lys Gly Gly Lys Ala Val Thr
100 105 110
Gln Thr Leu Thr His Val Gly Ala Ala Ser Val Asp Ala Arg Phe Ser
115 120 125
Ser Thr Thr Asn Ser Ile Thr Leu Arg Pro Ile Pro Ala Arg Gly Gly
130 135 140
Asp Pro Lys Lys Ile Lys Val Leu Arg Ser Arg Arg Thr His Phe Ala
145 150 155 160
Pro Arg Thr Ser His Phe Asp Arg His Asn Leu Thr Ser Ala Ser Asp
165 170 175
Pro Phe Arg Gly Leu Tyr Thr Leu Phe Trp Ile Val Ile Phe Val Gly
180 185 190
Ala Leu Lys Thr Val Tyr His Arg Phe Ala Glu Gln Gly Gly Trp Gly
195 200 205
Gly Glu Trp Arg Phe Ala Ala Leu Ile Ser Arg Asp Gly Trp Val Leu
210 215 220
Ala Val Ser Asp Ala Val Leu Val Ser Ala Ser Leu Leu Cys Val Pro
225 230 235 240
Tyr Ala Lys Leu Leu Val His Gly Trp Ile Arg Tyr His Gly Ala Gly
245 250 255
Val Ile Ile Gln His Ile Cys Gln Thr Leu Tyr Leu Ala Ile Ala Ile
260 265 270
Arg Trp Thr Phe His Arg Asn Trp Pro Trp Val Gln Ser Gly Phe Met
275 280 285
Thr Leu His Ala Leu Ser Met Leu Met Lys Ile His Ser Tyr Cys Ser
290 295 300
Leu Asn Gly Glu Leu Ser Glu Arg Arg Arg Gln Leu Lys Lys Asp Glu
305 310 315 320
Lys Arg Leu Glu Glu Val Leu Glu Glu Met Gly Gly Arg Arg Lys Ala
325 330 335
Glu Arg Glu Ala Arg Glu Glu Trp Glu Arg Gln Cys Gly Glu Ala Ala
340 345 350
Arg Ala Lys Glu Gly Glu Ala Gly Val Ser Glu Gly Glu Lys Glu Ala
355 360 365
Ala Ala Thr Leu Ser Ser Thr Asp Ala Ser Asn Ser Ala Leu Ser Ser
370 375 380
Glu Asp Glu Ala Ala Ala Ala Leu Leu Arg His Arg Gln Pro Thr Ala
385 390 395 400
Arg Arg Arg Ser Ile Ser Pro Ser Ala Ser Arg Thr Gly Ser Ser Ser
405 410 415
Ala Pro Ser Ala Thr Leu Ala Pro Ser Arg Ala Glu Glu Pro Gln Glu
420 425 430
Gly Val Glu Thr Leu Thr Trp His Pro Ser Asp Gln Val Ser Lys Leu
435 440 445
Ala Ile Ala Ile Cys Glu Ala Lys Asp Leu Leu Thr Ser Asn Gly Lys
450 455 460
Lys Pro Val Thr Phe Pro Glu Asn Val Thr Phe Ala Asn Phe Ile Asp
465 470 475 480
Tyr Leu Leu Val Pro Thr Leu Val Tyr Glu Leu Glu Tyr Pro Arg Thr
485 490 495
Asp Ser Ile Arg Pro Leu Tyr Ile Leu Glu Lys Thr Leu Ala Thr Phe
500 505 510
Gly Thr Phe Ser Ile Leu Val Leu Ile Val Asp Ser Phe Ile Leu Pro
515 520 525
Val Thr Ser Arg Thr Asp Thr Pro Leu Phe Gly Phe Val Leu Asp Leu
530 535 540
Ala Leu Pro Phe Thr Leu Ala Tyr Leu Leu Ile Phe Tyr Val Ile Phe
545 550 555 560
Glu Gly Val Cys Asn Gly Phe Ala Glu Leu Thr Arg Phe Ala Asp Arg
565 570 575
Asn Phe Phe Asp Asp Trp Trp Asn Ser Cys Thr Phe Asp Glu Phe Ser
580 585 590
Arg Lys Trp Asn Arg Pro Val His Ala Phe Leu Leu Arg His Val Tyr
595 600 605
Ala Glu Thr Met Ala Ser Tyr Lys Leu Ser Lys Leu Ser Ala Ala Phe
610 615 620
Val Thr Phe Leu Phe Ser Ala Cys Val His Glu Leu Val Met Ala Val
625 630 635 640
Val Thr Lys Lys Leu Arg Leu Tyr Leu Phe Ser Met Gln Met Ala Gln
645 650 655
Leu Pro Leu Ile Met Val Gly Arg Ala Lys Ile Phe Arg Gln Tyr Pro
660 665 670
Ala Leu Gly Asn Leu Phe Phe Trp Leu Ala Leu Leu Ser Gly Phe Pro
675 680 685
Leu Leu Gly Thr Leu Tyr Leu Arg Tyr
690 695
<210> 32
<211> 2094
<212> DNA
<213> 圆红冬孢酵母
<400> 32
atgacggagc gatcccttcc agtgacgctc cctcttcctc gaaactttgc gctcacaccg 60
caccagatgg cctcgccaga cccgccactc ccaggcccag ccaacctcgt cgacgacgca 120
ctccgacacc cagactcggc gccgcccatc tcgcccgact ccgcgcctcc ttcgactgcg 180
actcggccct ctgctctctc gcgcggagag ctctcgaccg cttcgagcta cgcgagcgag 240
gtgtcgacga gggaggggac accggatctg gcgaatgggc aaggggttac gacgaccatc 300
acgactgtca caggcaaagg cggaaaggcc gtcacccaga ccctcaccca cgtcggcgcc 360
gcctccgtcg acgcccgctt ctcctccacc acaaactcca tcactctccg ccctatcccc 420
gcccgtggcg gcgacccgaa aaagatcaaa gtcctccgct ctcgtcggac ccacttcgcc 480
ccacgcacct cacacttcga ccgtcacaac ctcacctccg cctctgaccc gttccgcgga 540
ctgtacacgt tgttctggat cgtgatcttc gttggggcac tcaagactgt gtatcatcgg 600
tttgcggaac agggtgggtg gggtggagaa tggaggtttg cggcgttgat tagtcgcgat 660
gggtgggttc tggcggttag tgatgcggtg ttggttagcg cgtcgttgtt gtgcgtgccg 720
tatgcaaagc tcctcgtaca cggctggatc cggtaccacg gcgcaggcgt catcatccaa 780
cacatctgtc aaacgctcta cctcgccatc gcgatccgct ggaccttcca ccgcaactgg 840
ccctgggtcc aaagcggttt catgaccctc cacgccctct cgatgctcat gaagatccat 900
agctactgtt ctctgaacgg cgagctttcg gagcggcgga gacagttgaa gaaggacgag 960
aagcggttgg aggaggtgct ggaggagatg ggtggacgga ggaaggcgga gagggaggcg 1020
agggaggagt gggagaggca gtgtggggag gcggcgaggg ccaaggaggg tgaggcggga 1080
gtgagcgagg gggagaagga ggcggcggcg actctatctt cgacggatgc gtcgaattcg 1140
gccctttcgt cggaggacga ggcggctgcg gcgctgttgc ggcatcgaca gccgactgct 1200
cgacgacgat ccatctcgcc ctctgcctca cgcaccggtt cctcctccgc cccctccgct 1260
accctcgccc cctctcgcgc cgaagaaccc caagaaggcg ttgagacgct cacctggcac 1320
ccatccgacc aagtcagcaa actcgctatc gccatctgcg aggcaaagga cctcctcacg 1380
agtaacggca agaagcccgt cacgttcccc gagaacgtca cctttgcgaa ctttatcgac 1440
tacttgcttg tgccgacgtt ggtgtacgag ttggagtacc ctcggacgga ttccatccgg 1500
cccctctaca tcctcgaaaa gaccctcgca accttcggca ccttctccat tctcgtcctc 1560
atcgtcgact cgttcatcct ccccgtcacc tcgcgcaccg acacgcccct cttcgggttc 1620
gtcctcgacc tcgccctgcc gttcacgctc gcgtacctcc tcatcttcta cgtcatcttt 1680
gagggcgtgt gcaatgggtt tgcggagttg acgaggtttg cggatcggaa tttcttcgac 1740
gattggtgga actcgtgcac gttcgacgag ttctcgcgca agtggaatcg ccccgtccac 1800
gccttcctcc tccgccacgt ttacgccgaa acgatggctt cttacaagct ctcgaagctc 1860
tcggctgcgt tcgtcacgtt cttgttcagc gcctgcgtgc acgaactcgt catggcggtc 1920
gtgacgaaga agcttcggct gtacctgttc tcgatgcaga tggcccagct cccgctcatc 1980
atggtgggcc gcgccaagat cttccgacag tatccagcgc tcggcaacct cttcttctgg 2040
ctcgcccttc tctcgggatt cccgcttctc gggacgctgt atctgcggta ctga 2094
<210> 33
<211> 555
<212> PRT
<213> 斯达氏油脂酵母
<400> 33
Met Ser Thr Ala Ala Gln Ser Asp Thr Asp Asn Glu Asp Ile Ser Thr
1 5 10 15
Val Asp Leu Val Asp Ser Arg Ala Asp Thr His Thr Ser Ser Asn Val
20 25 30
Met Leu Gln Gln Gln Lys Ser Arg Arg Arg Leu Ile Gly Lys Asp Ala
35 40 45
Glu Pro Arg Thr Gln His Pro Ser Gly Gly Lys Ser Glu Lys Glu Glu
50 55 60
Leu Thr Lys Pro Asp Asp Ser Lys Gly Pro Ile Lys Leu Ser His Ile
65 70 75 80
Tyr Pro Ile His Ala Val Ser Arg Gly Ser Ile Leu Ser Arg Glu Ser
85 90 95
Thr Thr Pro Thr Pro Ser Phe Val Gly Phe Arg Asn Leu Ala Met Ile
100 105 110
Val Leu Gly Lys Leu Gln Tyr Ser Leu Phe Phe Trp Cys Asp Arg Ala
115 120 125
Asn Ile Pro Thr Ala Val Ser Asn Leu Arg Leu Val Ile Glu Asn Tyr
130 135 140
Ser Lys Tyr Gly Val Leu Ile Arg Phe Ala Arg Leu Gly Ile Ser Gln
145 150 155 160
Lys Asp Ile Leu Tyr Cys Ile Phe Leu Thr Ala Thr Ile Pro Leu His
165 170 175
Leu Phe Ile Ala Ile Val Ile Glu Arg Leu Val Ala Ile Pro Thr Val
180 185 190
Asn Tyr Val Ala Ser Leu Ser Glu Ser Glu Asp Lys Lys Arg Ser Asn
195 200 205
Pro Lys Met Gly Arg Lys Gly Gly Ser Ile Ser Ile Leu Arg Pro Lys
210 215 220
Pro Lys Tyr Met Trp Arg Leu Ile Val Leu Leu His Ser Ile Asn Ala
225 230 235 240
Met Ala Cys Leu Trp Val Thr Thr Val Val Val Tyr Asn Ser Ile Tyr
245 250 255
His Pro Leu Ile Gly Thr Ala Cys Glu Phe His Ala Val Ile Val Cys
260 265 270
Leu Lys Val Ala Ser Phe Ala Leu Thr Asn Arg Asp Leu Arg Glu Ser
275 280 285
Met Leu Asn Ser Gln Pro Val Pro Ala Ile Tyr Asn Leu Ala Pro Tyr
290 295 300
Pro Lys Asn Leu Thr Leu Lys Asn Leu Ser Tyr Phe Trp Trp Ala Pro
305 310 315 320
Thr Leu Val Tyr Gln Pro Val Tyr Pro Arg Ser Pro Ser Phe Arg Pro
325 330 335
Leu Phe Phe Val Lys Arg Ile Leu Glu Met Val Gly Leu Ser Phe Leu
340 345 350
Ile Trp Phe Leu Ser Ala Gln Tyr Ala Val Pro Thr Leu Glu Asn Ser
355 360 365
Leu Val His Phe His Ser Leu Gln Phe Met Gly Ile Met Glu Arg Leu
370 375 380
Met Lys Leu Ala Ser Ile Ser Met Ala Ile Trp Leu Ala Gly Phe Phe
385 390 395 400
Cys Ile Phe Gln Ser Gly Leu Asn Ala Leu Ala Glu Val Met Arg Phe
405 410 415
Gly Asp Arg Ala Phe Tyr Asp Asp Trp Trp Asn Ser Lys Ser Val Gly
420 425 430
Glu Tyr Trp Arg Leu Trp Asn Lys Pro Val Thr Asn Tyr Phe Arg Arg
435 440 445
His Ile Tyr Val Pro Leu Val Arg Arg Gly Trp Asn Ser Ala Thr Ala
450 455 460
Ser Val Met Val Phe Phe Val Ser Ala Val Leu His Glu Leu Val Val
465 470 475 480
Gly Val Pro Thr His Asn Val Ile Gly Val Ala Phe Ser Ser Met Ile
485 490 495
Leu Gln Ile Pro Leu Ile Gln Val Thr Ala Pro Leu Glu Lys Met His
500 505 510
Gly Pro Thr Ser Gly Ile Ile Gly Asn Cys Ile Phe Trp Phe Ser Phe
515 520 525
Phe Ile Gly Gln Pro Leu Gly Val Leu Leu Tyr Tyr Phe Ala Trp Asn
530 535 540
Val Ser Met Ser Lys Val Lys Met Val Glu Ser
545 550 555
<210> 34
<211> 1668
<212> DNA
<213> 斯达氏油脂酵母
<400> 34
atgtcgaccg ctgcacaatc tgatacagac aacgaggata tatcgactgt cgatttggtt 60
gactctcgtg cagatactca cacatcttca aatgttatgt tgcaacagca aaaatcgcgt 120
cggagactaa tcgggaaaga cgccgagcca agaacacagc atccgtctgg aggcaaatcg 180
gagaaggagg agttgacgaa gccggatgac tcaaagggac ccataaaatt aagtcacata 240
tacccgatac atgccgttag ccgaggcagt attctgtcac gagagtcgac aactcctaca 300
ccgagttttg ttgggtttcg aaacttagcc atgatagtgc tagggaagtt acagtattca 360
ttattctttt ggtgcgatcg ggctaacatt ccgacagccg tcagcaatct tcgattggtg 420
attgaaaatt actcaaagta cggcgttctg atccgattcg cccgactcgg tatttcacaa 480
aaggacattc tgtattgcat attcttgacc gctaccatcc cgctgcacct atttattgct 540
attgtcattg aaagactagt tgcgattccg acggtaaact acgtcgcttc gctcagcgag 600
agcgaggata aaaaacgctc caaccccaaa atgggacgga aggggggcag tatatcgatt 660
ttgcgtccta agccaaaata tatgtggcgc ctgatcgtcc tattgcattc aataaacgca 720
atggcttgct tgtgggttac gactgttgtt gtttacaatt ctatttatca tccccttatt 780
gggacagctt gtgaatttca tgcagtgatt gtgtgtctta aggtcgcatc gtttgcgctt 840
accaatcgcg atcttcggga gtcgatgctg aactctcaac ctgtgccagc catatacaac 900
ttggcccctt atccaaaaaa cttaaccctc aagaacttgt catacttttg gtgggcgccg 960
actcttgttt atcaacctgt ctatccgcga tcgccttcat tccggccttt gttttttgtc 1020
aagcggattc tggagatggt gggcctatca tttttaatat ggttcttgtc agctcaatat 1080
gctgtgccga cgctagaaaa tagtttggtg cattttcaca gtttgcaatt catgggaatt 1140
atggagcgac tcatgaagct tgctagcatt agcatggcta tttggcttgc tggttttttc 1200
tgcatttttc agtctggact caatgcgctt gcggaggtaa tgcggtttgg tgacagagcc 1260
ttttacgacg actggtggaa cagcaaatct gtgggagagt attggcgtct gtggaataag 1320
ccggttacga attacttccg gcgtcatatt tacgtaccgc ttgtgcgccg cgggtggaat 1380
tctgcgacag ccagtgtcat ggtatttttc gtcagcgcgg tgttgcatga gctagttgtt 1440
ggagttccga cgcataacgt aattggagtt gcattctcgt cgatgattct acaaatccca 1500
ctcatacaag taaccgcgcc tctggagaag atgcatggac ctacatctgg aataataggg 1560
aactgtatct tttggtttag cttcttcatc ggtcagcctc tgggcgtgct actttactat 1620
tttgcgtgga acgttagtat gagcaaagta aagatggtcg agagctag 1668
<210> 35
<211> 517
<212> PRT
<213> 土曲霉
<400> 35
Met Val Met Asp Thr Gln Thr Thr Ala Ser Ala Thr Ser Thr Ala Leu
1 5 10 15
Thr Thr Asp His Thr Val Ala Ser Arg Thr Ser Arg Ser Glu Pro Asn
20 25 30
Gly Gly Val His Asn Val Ser Ser Pro Pro Thr Ser Glu Pro Thr Gly
35 40 45
Gly Asn Gly Gly Gly Arg Arg Arg Ser Lys Tyr Arg His Val Ala Ala
50 55 60
Tyr His Ser Glu Val Arg His Ser Ser Leu Ser Arg Glu Ser Asn Thr
65 70 75 80
Ser Pro Ser Phe Leu Gly Phe Arg Asn Leu Met Val Ile Val Leu Gly
85 90 95
Glu Cys Pro Ser Ala Leu Leu Arg Phe Val Asn Pro Thr Glu Asn Ser
100 105 110
Tyr Gly Ser Arg Leu Val Ala Met Asn Leu Arg Leu Val Ile Glu Asn
115 120 125
Tyr Val Lys Tyr Gly Val Leu Ile Cys Ile Arg Cys His Asp Tyr Arg
130 135 140
Lys Gln Asp Val Val Leu Gly Ser Met Leu Phe Ala Leu Val Pro Cys
145 150 155 160
Gln Leu Phe Ile Ala Tyr Leu Leu Glu Leu Ala Ala Ala Gly Arg Ala
165 170 175
Lys Gln Thr Val Gly Arg Lys Lys Lys Asp Gly Ser Ala Glu Glu Gly
180 185 190
Glu Arg Glu Ala Arg Ala Phe Arg His Ile Trp Arg Phe Ala Leu Ser
195 200 205
Phe His Ile Leu Asn Ile Val Leu Asn Leu Ala Val Thr Ser Phe Val
210 215 220
Val Tyr Tyr Tyr Ile His His Pro Gly Ile Gly Thr Leu Cys Glu Val
225 230 235 240
His Ala Ile Val Val Ala Leu Lys Asn Trp Ser Tyr Ala Phe Thr Asn
245 250 255
Arg Asp Leu Arg Glu Ala Met Leu Asn Pro Ser Ala Glu Ser Ala Leu
260 265 270
Pro Glu Ile Tyr Ser Ser Leu Pro Tyr Pro Lys Asn Ile Thr Leu Gly
275 280 285
Asn Leu Thr Tyr Phe Trp Leu Ala Pro Thr Leu Leu Tyr Gln Pro Val
290 295 300
Tyr Pro Arg Ser Pro Ser Ile Arg Trp Pro Phe Val Ala Lys Arg Leu
305 310 315 320
Ser Glu Phe Ala Cys Leu Ser Val Phe Ile Trp Leu Leu Ser Ala Gln
325 330 335
Tyr Ala Ala Pro Val Leu Arg Asn Ser Ile Asp Lys Ile Arg Asp Met
340 345 350
Ala Tyr Ala Ser Ile Phe Glu Arg Val Met Lys Leu Ser Thr Ile Ser
355 360 365
Leu Val Ile Trp Leu Ala Gly Phe Phe Ala Ile Phe Gln Ser Leu Leu
370 375 380
Asn Ala Leu Ala Glu Ile Met Lys Phe Gly Asp Arg Glu Phe Tyr Thr
385 390 395 400
Asp Trp Trp Asn Ser Pro Ser Leu Gly Val Tyr Trp Arg Ser Trp Asn
405 410 415
Arg Pro Val Tyr Gln Phe Met Lys Arg His Val Tyr Ser Pro Leu Ile
420 425 430
Gly Arg Gly Tyr Ser Pro Phe Val Ala Ser Thr Val Val Phe Thr Ile
435 440 445
Ser Ala Leu Leu His Glu Leu Leu Val Gly Ile Pro Thr His Asn Met
450 455 460
Ile Gly Val Ala Leu Val Gly Met Leu Phe Gln Leu Pro Leu Ile Ala
465 470 475 480
Ile Thr Ala Pro Leu Glu Lys Met Lys Asp Pro Leu Gly Lys Pro Leu
485 490 495
Gly Ala Leu Leu Tyr Phe Phe Ala Trp Gln Ala Lys Tyr Gly Ser Val
500 505 510
Ser Arg Met Gly Asn
515
<210> 36
<211> 1554
<212> DNA
<213> 土曲霉
<400> 36
atggtgatgg acacacaaac cacagcatcc gccaccagca cggcgctcac gaccgaccac 60
actgttgcct ctcggacgtc ccgctctgag ccgaacggtg gtgtgcataa tgtatcgtca 120
cctccaacga gcgaaccgac tgggggaaat ggcggaggcc ggcgaaggag taaataccgg 180
catgtcgcag cgtaccattc cgaagtgcgc cattccagtc tcagtcggga atcgaatact 240
tctccgagtt tcctcggatt ccggaacctc atggtaatcg tattaggtga gtgccctagt 300
gctctcctac gttttgtgaa cccgacggag aactcatacg ggtcgcgact agttgctatg 360
aatcttcgat tggttatcga gaattacgtg aagtatgggg tcttgatctg catcagatgc 420
cacgattatc gaaagcagga cgttgtcctg ggctcaatgt tatttgctct cgtcccatgc 480
cagctattca tcgcctacct cctggaattg gccgcagcgg gtagggccaa acagactgtg 540
ggccgaaaga aaaaggacgg atcagccgag gagggcgaac gtgaagcacg tgcttttcga 600
cacatctggc ggtttgcatt gtcctttcac atcctcaaca ttgttctcaa tctcgccgtc 660
acgagcttcg ttgtgtatta ctacatccac catcccggca ttggtacgct ctgtgaagtg 720
catgcgatcg ttgtcgcgtt gaaaaactgg tcctatgcgt tcaccaatcg ggatctgcga 780
gaggcgatgc ttaatccctc ggcggagtcg gcgcttcccg agatctattc cagcctcccg 840
tacccgaaaa acatcacgtt aggaaatcta acgtacttct ggcttgcacc gacactgttg 900
tatcagccag tataccccag gtcgccttcc atccgatggc cattcgtggc caaacgcttg 960
tcggaatttg cgtgcttgtc ggtgttcatt tggctacttt cggcccaata cgctgcgcca 1020
gttttgcgca actccattga caagattcgt gatatggcat atgcatccat ttttgagcgc 1080
gttatgaagc tatccaccat ctctctcgtc atttggctgg ctgggttctt tgcgattttc 1140
caatcactct tgaatgcttt ggcggagatc atgaagtttg gcgatcggga attctacacc 1200
gattggtgga atagcccaag tctcggtgtt tactggcggt catggaatcg gccagtgtac 1260
cagttcatga agcggcacgt atattctccg ttgatagggc gggggtacag cccgtttgtg 1320
gcaagcactg tcgtattcac catctccgct ctccttcatg agctcctcgt ggggataccc 1380
acgcacaaca tgataggcgt cgcgcttgtt ggaatgctgt tccagctccc gttgatcgcc 1440
atcactgccc cattggaaaa gatgaaagat ccattgggta agcccctggg agcactgctg 1500
tatttctttg cctggcaggc aaaatatggc agtgtgagca ggatgggcaa ctga 1554
<210> 37
<211> 506
<212> PRT
<213> 紫色麦角菌
<400> 37
Met Ser Ala Thr Gly Val Asp Val Ala Asn Gly Arg Ser Gly Ala Arg
1 5 10 15
Arg Arg Asn Asp Thr Ala Val Asp Glu Thr Ile Ser Ala Val Thr Ala
20 25 30
Glu Met Arg Ser Ser Ser His Pro Thr Tyr Arg His Val Ser Ala Val
35 40 45
His Ser Thr Ser Arg Pro Ser Cys Leu Ser His Asp Ser Asp Ala Ala
50 55 60
Pro Ser Phe Ile Gly Phe Arg Asn Leu Met Val Ile Val Leu Val Val
65 70 75 80
Gly Asn Val Arg Leu Met Ile Glu Asn Leu Lys Lys Tyr Gly Val Leu
85 90 95
Ile Cys Leu Arg Cys His Ser Tyr Lys Asn Glu Asp Ile Ile Ile Gly
100 105 110
Gly Leu Leu Tyr Phe Leu Ile Pro Cys His Leu Leu Val Ala Tyr Gly
115 120 125
Ile Glu Leu Ala Ala Ala Arg Gln Ala Arg Glu Ser Arg Thr Arg Pro
130 135 140
Pro Gly Gln Ser Asp Thr Ala Ser Lys Ser Thr Glu Asp Asp Asn Lys
145 150 155 160
His Phe His Ser Thr Trp Val Leu Ala Ala Trp Ala His Ile Ile Asn
165 170 175
Met Thr Leu Ser Phe Ile Leu Thr Thr Phe Val Val Tyr Tyr Tyr Val
180 185 190
His His Pro Leu Val Gly Thr Leu Thr Glu Met His Ala Val Ile Val
195 200 205
Ser Leu Lys Thr Ala Ser Tyr Ala Phe Thr Asn Arg Asp Leu Arg His
210 215 220
Ala Tyr Leu His Pro Asp Lys Arg Lys His Ile Pro Glu Leu Tyr Leu
225 230 235 240
Glu Cys Pro Tyr Pro Gln Asn Leu Thr Phe Gly Asn Leu Val Tyr Phe
245 250 255
Trp Trp Ala Pro Thr Leu Val Tyr Gln Pro Val Tyr Pro Arg Thr Asp
260 265 270
Lys Ile Arg Trp Val Phe Val Phe Lys Arg Leu Gly Glu Val Cys Cys
275 280 285
Leu Ser Ala Phe Ile Trp Phe Ala Ser Phe Gln Tyr Ala Ala Pro Val
290 295 300
Leu Arg Asn Ser Leu Asp Lys Ile Ala Ser Leu Asp Phe Ile Met Ile
305 310 315 320
Phe Glu Arg Leu Leu Lys Leu Ser Thr Ile Ser Leu Val Ile Trp Leu
325 330 335
Ala Gly Phe Phe Ala Leu Phe Gln Ser Phe Leu Asn Ala Leu Ala Glu
340 345 350
Val Leu Arg Phe Gly Asp Arg Cys Phe Tyr Asp Asp Trp Trp Asn Ser
355 360 365
Glu Ser Leu Gly Ala Tyr Trp Arg Thr Trp Asn Arg Pro Val Tyr Thr
370 375 380
Tyr Phe Lys Arg His Val Tyr Val Pro Met Ile Gly Arg Gly Trp Ser
385 390 395 400
Pro Trp Thr Ala Ser Cys Thr Val Phe Phe Val Ser Ala Val Leu His
405 410 415
Glu Val Leu Val Gly Val Pro Thr His Asn Ile Ile Gly Val Ala Phe
420 425 430
Val Gly Met Phe Leu Gln Leu Pro Leu Ile Ala Leu Thr Ala Pro Met
435 440 445
Glu Lys Lys Lys Trp Gly His Thr Gly Arg Val Met Gly Asn Val Ile
450 455 460
Phe Trp Val Ser Phe Thr Ile Phe Gly Gln Pro Phe Ala Ala Leu Met
465 470 475 480
Tyr Phe Tyr Ala Trp Gln Ala Lys Tyr Gly Ser Val Ser Arg Gln Ile
485 490 495
Val Leu Val Asn Pro Val Glu Glu Ala Ser
500 505
<210> 38
<211> 1521
<212> DNA
<213> 紫色麦角菌
<400> 38
atgtccgcca cgggcgttga tgtggccaac ggccgcagcg gcgcgcgacg acgcaacgat 60
actgccgtcg acgagactat atccgccgtc acggccgaga tgcgttcctc gtcgcatcca 120
acataccgcc atgtgtctgc tgtgcactcc acgagccggc cctcgtgtct gagccatgat 180
tctgacgctg cgccgagctt cattggcttt cgaaatctca tggtcattgt tctggtcgtt 240
ggcaatgttc gattaatgat tgaaaatcta aaaaagtacg gcgtactgat atgcctccga 300
tgtcactcgt ataaaaacga agacatcatt atcggcggac tgctctactt cctgatcccc 360
tgccacttgc ttgtcgccta cggaatcgag ttagccgccg ccagacaagc acgcgaatct 420
cgaactcgtc caccaggcca gtccgacacg gcgtcgaaat caacagaaga tgacaacaag 480
cacttccact caacatgggt gctcgctgcc tgggcacaca tcatcaacat gacactttcc 540
ttcatcctca ccaccttcgt cgtctactac tacgtgcacc atcccctcgt cggcaccctg 600
accgagatgc acgccgtcat cgtctctctc aaaacagctt cctacgcatt caccaaccga 660
gatcttcgcc acgcatacct ccatcctgac aagcgcaagc acatccccga gctatatctc 720
gaatgtccct acccccagaa cctcaccttt ggcaatctcg tgtatttctg gtgggccccc 780
acgctggtat accagcccgt gtatccgcgc accgacaaga tcagatgggt ttttgttttt 840
aaaagactag gcgaagtctg ctgtctcagc gcattcatct ggttcgccag cttccagtac 900
gccgcgcccg tgttgcggaa ctccctggac aagattgcgt ctctcgactt catcatgatc 960
tttgagcgcc ttctcaagct atccaccatt tctctcgtca tctggctcgc cggcttcttc 1020
gccctgttcc agtctttcct gaatgccctg gctgaggtat tgcgctttgg ggaccggtgc 1080
ttctacgacg attggtggaa tagcgagagt ctgggggcgt attggaggac gtggaacagg 1140
cctgtgtata cctacttcaa gcgccatgtg tatgtgccca tgattgggag gggatggagt 1200
ccctggactg ctagttgtac tgtttttttt gtgtcggcgg tgctgcacga ggttcttgtt 1260
ggggtgccca cccacaatat cattggtgtc gcctttgtgg gcatgtttct gcagcttccc 1320
ctaatagccc tcaccgctcc catggaaaag aagaaatggg gccacaccgg ccgtgtgatg 1380
ggcaatgtta ttttctgggt gtcctttaca atctttgggc agccctttgc agcgctcatg 1440
tacttttatg cctggcaggc caagtacggg agcgtgagtc ggcaaattgt gctggtgaat 1500
ccggtggagg aggcgtcttg a 1521
<210> 39
<211> 551
<212> PRT
<213> 球毛壳霉
<400> 39
Met Lys Ala Glu Thr Gly Thr Thr Met Ala Thr Ser Thr Ser Leu Glu
1 5 10 15
Thr Ser Gln Val Asn Gly Val Thr Asn Arg Ala Pro Val Gly Pro Ser
20 25 30
His Asp Pro His Ala Thr Thr Pro Thr His Glu Thr Thr Thr Thr Ile
35 40 45
Pro Ser Asp Val Leu Ala Asn Gly Ser Thr Asn Gly Thr Thr Asn Gly
50 55 60
Thr Thr Asp Asp Ser Leu Asp Ile Ser Glu Leu Arg Lys Ala Phe Arg
65 70 75 80
Asn Lys Tyr Arg His Val Glu Ala Val His Ser Glu Ser Lys Pro Ser
85 90 95
Cys Leu Ser His Asp Ala Thr Glu Thr Pro Ser Phe Ile Gly Phe Arg
100 105 110
Asn Leu Met Val Ile Val Leu Val Ala Ala Asn Leu Arg Leu Val Ile
115 120 125
Glu Asn Ile Gln Lys Tyr Gly Val Leu Ile Cys Ile Lys Cys His Asp
130 135 140
Phe Arg Pro Asn Asp Val Arg Leu Gly Leu Leu Leu Tyr Ile Leu Ile
145 150 155 160
Pro Trp His Leu Met Leu Ala Tyr Leu Ile Glu Leu Val Ala Ala Ala
165 170 175
Asn Ala Arg Asn Ser Arg Ala Lys Ala Lys Lys Arg Asp Gly Ser Thr
180 185 190
Ser Pro Thr Glu Asp Glu Ser Lys Gln Phe Leu Gln Thr Trp Arg Met
195 200 205
Leu Arg Ile Leu His Ala Val Asn Val Thr Ala Ala Leu Ala Val Thr
210 215 220
Ser Tyr Val Val Tyr Tyr Tyr Ile His His Pro Leu Ile Gly Thr Leu
225 230 235 240
Ser Glu Leu His Ala Ile Ile Val Trp Leu Lys Thr Ala Ser Tyr Ala
245 250 255
Leu Thr Asn Arg Asp Leu Arg His Ala Tyr Leu His Pro Val Arg Gly
260 265 270
Glu Arg Asp Ala Leu Pro Glu Ile Tyr Ala Gln Cys Pro Tyr Pro Ala
275 280 285
Asn Val Thr Phe Ser Asn Leu Thr Tyr Phe Trp Trp Ala Pro Thr Leu
290 295 300
Val Tyr Gln Pro Ala Tyr Pro Arg Thr Gln Arg Ile Arg Trp Val Phe
305 310 315 320
Val Ala Lys Arg Leu Gly Glu Val Val Cys Leu Ser Ala Phe Ile Trp
325 330 335
Phe Ala Ser Ala Gln Tyr Ala Thr Pro Val Leu Arg Asn Ser Leu Asp
340 345 350
Lys Ile Ala Thr Leu Asp Tyr Met Ser Ile Val Glu Arg Leu Leu Lys
355 360 365
Leu Ser Thr Ile Ser Leu Val Ile Trp Leu Ala Gly Phe Phe Ala Leu
370 375 380
Phe Gln Ser Phe Leu Asn Ala Leu Ala Glu Val Met Arg Phe Gly Asp
385 390 395 400
Arg Glu Phe Tyr Glu Ala Trp Trp Asn Ser Glu Ser Leu Gly Ala Tyr
405 410 415
Trp Arg Thr Trp Asn Lys Pro Val Tyr Gln Phe Phe Arg Arg His Val
420 425 430
Tyr Ser Pro Met Arg Ser Arg Gly Trp Ser His Leu Ser Ala Ser Leu
435 440 445
Ala Val Phe Leu Leu Ser Ala Val Leu His Glu Leu Leu Val Gly Val
450 455 460
Pro Thr His Asn Ile Ile Gly Val Ala Phe Leu Gly Met Phe Leu Gln
465 470 475 480
Leu Pro Leu Ile Ala Met Thr Ala Arg Leu Gly Gly Arg Arg Gly Asn
485 490 495
Thr Ala His Gly Arg Leu Leu Gly Asn Thr Ile Phe Trp Val Ser Phe
500 505 510
Thr Ile Phe Gly Gln Pro Phe Ala Ala Leu Met Tyr Phe Tyr Ala Trp
515 520 525
Gln Ala Lys Tyr Gly Ser Val Ser Lys Met Pro Leu Ala Gln Pro Gly
530 535 540
Thr Cys Pro Ala Val Val Val
545 550
<210> 40
<211> 1656
<212> DNA
<213> 球毛壳霉
<400> 40
atgaaggcag aaacgggcac aacgatggca acgtcgacta gtctcgagac ttcccaagtc 60
aatggcgtca ccaaccgggc ccctgttggc cctagtcacg acccccacgc tacaactccg 120
actcatgaga cgacaaccac cataccgtcc gacgtcctcg ccaatggttc tacaaatggg 180
actacgaatg ggacgacaga tgattcattg gacatatccg aattgcgcaa agcgttccgc 240
aacaagtatc gccatgtcga ggctgtccac tccgaatcga aaccatcctg tctgagccat 300
gacgctacag agacacccag tttcatcggt tttaggaatc tcatggtgat tgtgttggtt 360
gctgccaatc ttcgcctggt catcgagaac attcaaaagt atggagttct gatctgcatc 420
aaatgccacg actttcgccc caacgatgta cgcctggggc tcctcctcta catcctgatc 480
ccatggcacc tcatgctcgc ctacctcatt gagctggtcg ccgccgccaa tgcccgcaac 540
tcccgggcca aggcgaagaa gcgggacggc agtaccagcc cgaccgaaga cgagtccaag 600
caattcctgc agacctggcg gatgctccgc attctccacg ccgtcaacgt cacggccgcc 660
ctggccgtca cctcctacgt ggtctactac tacattcacc acccgctgat cggcacgctc 720
tcggagctgc acgccatcat cgtgtggctc aagacggcgt cgtacgcgct caccaaccgc 780
gacctgcgcc acgcctacct acacccggtg cgcggcgagc gcgacgctct gcccgagatc 840
tacgcccagt gcccctaccc ggccaacgtg accttctcca acttgaccta cttctggtgg 900
gcgcccaccc tggtgtacca gccggcgtac ccgcgcactc agcgcatccg ctgggtcttt 960
gtggctaagc gcctcggcga ggtcgtctgc ttgagcgcct tcatctggtt cgccagcgcc 1020
cagtacgcta cccccgtgct gcgaaactcg ctcgacaaga tcgctaccct ggattacatg 1080
tccattgtcg agcgtctgtt gaagctgtcg accatctcgc tggtcatctg gctggcgggc 1140
ttctttgcgc tgtttcagag tttcctgaat gccttggccg aggtgatgcg gtttggagac 1200
cgcgagttct acgaagcatg gtggaacagc gaaagcctcg gcgcctactg gcgcacctgg 1260
aacaaacccg tgtaccaatt cttccggcgg cacgtctact cgccgatgcg gtcgcgcggg 1320
tggagccact tgtcggccag cctcgccgtg tttctgctct cggccgtgct acacgagctg 1380
ctggtggggg tgccgacgca caacatcatc ggcgtcgcct tcctgggcat gttcctgcag 1440
ctgccgctca tcgccatgac ggcgcgcctg ggcggccgcc gcgggaacac cgcccacggc 1500
cgcctgctcg gcaacactat cttttgggtg tcatttacca tttttggcca gccgtttgcc 1560
gcgctgatgt atttttatgc atggcaggcc aagtatggta gtgtgagcaa gatgccgctg 1620
gcgcagccgg ggacgtgtcc ggctgtggtt gtttga 1656
<210> 41
<211> 759
<212> PRT
<213> 酿酒酵母
<400> 41
Met Pro Ala Pro Lys Leu Thr Glu Lys Phe Ala Ser Ser Lys Ser Thr
1 5 10 15
Gln Lys Thr Thr Asn Tyr Ser Ser Ile Glu Ala Lys Ser Val Lys Thr
20 25 30
Ser Ala Asp Gln Ala Tyr Ile Tyr Gln Glu Pro Ser Ala Thr Lys Lys
35 40 45
Ile Leu Tyr Ser Ile Ala Thr Trp Leu Leu Tyr Asn Ile Phe His Cys
50 55 60
Phe Phe Arg Glu Ile Arg Gly Arg Gly Ser Phe Lys Val Pro Gln Gln
65 70 75 80
Gly Pro Val Ile Phe Val Ala Ala Pro His Ala Asn Gln Phe Val Asp
85 90 95
Pro Val Ile Leu Met Gly Glu Val Lys Lys Ser Val Asn Arg Arg Val
100 105 110
Ser Phe Leu Ile Ala Glu Ser Ser Leu Lys Gln Pro Pro Ile Gly Phe
115 120 125
Leu Ala Ser Phe Phe Met Ala Ile Gly Val Val Arg Pro Gln Asp Asn
130 135 140
Leu Lys Pro Ala Glu Gly Thr Ile Arg Val Asp Pro Thr Asp Tyr Lys
145 150 155 160
Arg Val Ile Gly His Asp Thr His Phe Leu Thr Asp Cys Met Pro Lys
165 170 175
Gly Leu Ile Gly Leu Pro Lys Ser Met Gly Phe Gly Glu Ile Gln Ser
180 185 190
Ile Glu Ser Asp Thr Ser Leu Thr Leu Arg Lys Glu Phe Lys Met Ala
195 200 205
Lys Pro Glu Ile Lys Thr Ala Leu Leu Thr Gly Thr Thr Tyr Lys Tyr
210 215 220
Ala Ala Lys Val Asp Gln Ser Cys Val Tyr His Arg Val Phe Glu His
225 230 235 240
Leu Ala His Asn Asn Cys Ile Gly Ile Phe Pro Glu Gly Gly Ser His
245 250 255
Asp Arg Thr Asn Leu Leu Pro Leu Lys Ala Gly Val Ala Ile Met Ala
260 265 270
Leu Gly Cys Met Asp Lys His Pro Asp Val Asn Val Lys Ile Val Pro
275 280 285
Cys Gly Met Asn Tyr Phe His Pro His Lys Phe Arg Ser Arg Ala Val
290 295 300
Val Glu Phe Gly Asp Pro Ile Glu Ile Pro Lys Glu Leu Val Ala Lys
305 310 315 320
Tyr His Asn Pro Glu Thr Asn Arg Asp Ala Val Lys Glu Leu Leu Asp
325 330 335
Thr Ile Ser Lys Gly Leu Gln Ser Val Thr Val Thr Cys Ser Asp Tyr
340 345 350
Glu Thr Leu Met Val Val Gln Thr Ile Arg Arg Leu Tyr Met Thr Gln
355 360 365
Phe Ser Thr Lys Leu Pro Leu Pro Leu Ile Val Glu Met Asn Arg Arg
370 375 380
Met Val Lys Gly Tyr Glu Phe Tyr Arg Asn Asp Pro Lys Ile Ala Asp
385 390 395 400
Leu Thr Lys Asp Ile Met Ala Tyr Asn Ala Ala Leu Arg His Tyr Asn
405 410 415
Leu Pro Asp His Leu Val Glu Glu Ala Lys Val Asn Phe Ala Lys Asn
420 425 430
Leu Gly Leu Val Phe Phe Arg Ser Ile Gly Leu Cys Ile Leu Phe Ser
435 440 445
Leu Ala Met Pro Gly Ile Ile Met Phe Ser Pro Val Phe Ile Leu Ala
450 455 460
Lys Arg Ile Ser Gln Glu Lys Ala Arg Thr Ala Leu Ser Lys Ser Thr
465 470 475 480
Val Lys Ile Lys Ala Asn Asp Val Ile Ala Thr Trp Lys Ile Leu Ile
485 490 495
Gly Met Gly Phe Ala Pro Leu Leu Tyr Ile Phe Trp Ser Val Leu Ile
500 505 510
Thr Tyr Tyr Leu Arg His Lys Pro Trp Asn Lys Ile Tyr Val Phe Ser
515 520 525
Gly Ser Tyr Ile Ser Cys Val Ile Val Thr Tyr Ser Ala Leu Ile Val
530 535 540
Gly Asp Ile Gly Met Asp Gly Phe Lys Ser Leu Arg Pro Leu Val Leu
545 550 555 560
Ser Leu Thr Ser Pro Lys Gly Leu Gln Lys Leu Gln Lys Asp Arg Arg
565 570 575
Asn Leu Ala Glu Arg Ile Ile Glu Val Val Asn Asn Phe Gly Ser Glu
580 585 590
Leu Phe Pro Asp Phe Asp Ser Ala Ala Leu Arg Glu Glu Phe Asp Val
595 600 605
Ile Asp Glu Glu Glu Glu Asp Arg Lys Thr Ser Glu Leu Asn Arg Arg
610 615 620
Lys Met Leu Arg Lys Gln Lys Ile Lys Arg Gln Glu Lys Asp Ser Ser
625 630 635 640
Ser Pro Ile Ile Ser Gln Arg Asp Asn His Asp Ala Tyr Glu His His
645 650 655
Asn Gln Asp Ser Asp Gly Val Ser Leu Val Asn Ser Asp Asn Ser Leu
660 665 670
Ser Asn Ile Pro Leu Phe Ser Ser Thr Phe His Arg Lys Ser Glu Ser
675 680 685
Ser Leu Ala Ser Thr Ser Val Ala Pro Ser Ser Ser Ser Glu Phe Glu
690 695 700
Val Glu Asn Glu Ile Leu Glu Glu Lys Asn Gly Leu Ala Ser Lys Ile
705 710 715 720
Ala Gln Ala Val Leu Asn Lys Arg Ile Gly Glu Asn Thr Ala Arg Glu
725 730 735
Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu
740 745 750
Glu Gly Lys Glu Gly Asp Ala
755
<210> 42
<211> 2280
<212> DNA
<213> 酿酒酵母
<400> 42
atgcctgcac caaaactcac ggagaaattt gcctcttcca agagcacaca gaaaactacg 60
aattacagtt ccatcgaggc caaaagcgtc aagacgtcgg ctgatcaggc atacatctac 120
caagagccta gcgctaccaa gaagatactt tactccatcg ccacatggct gttgtacaac 180
atcttccact gcttctttag agaaatcaga ggccggggca gtttcaaggt accgcaacag 240
ggaccggtga tctttgttgc ggctccgcat gctaaccagt tcgtcgaccc tgtaatcctt 300
atgggcgagg tgaagaaatc tgtcaacaga cgtgtgtcct tcttgattgc ggagagctca 360
ttaaagcaac cccccatagg gtttttggct agtttcttca tggccatagg cgtggtaagg 420
ccgcaggata atttgaaacc ggcagaaggt actatccgcg tagatccaac agactacaag 480
agagttatcg gccacgacac gcatttcttg actgattgta tgccaaaggg tctcatcggg 540
ttacccaaat caatgggatt tggagaaatc cagtccatag aaagtgacac gagtttgacc 600
ctaagaaaag agttcaaaat ggccaaacca gagattaaaa ctgctttact caccggcact 660
acttataaat atgccgctaa agtcgaccaa tcttgcgttt accatagagt ttttgagcat 720
ttggcccata acaactgcat tgggatcttt cctgaaggtg ggtcccacga cagaacaaac 780
ttgttgcccc tgaaagcagg tgtggcgatt atggctcttg gttgcatgga taagcatcct 840
gacgtcaatg ttaagattgt tccctgcggt atgaattatt tccatccaca taagttcagg 900
tcgagagcgg ttgttgaatt cggtgacccc attgaaatac cgaaggaact agtcgccaag 960
taccacaacc cggaaacgaa cagagatgca gtgaaagaat tattagatac catatcgaag 1020
ggtttacaat ccgttaccgt tacatgttct gattatgaaa ctttgatggt ggttcaaacg 1080
ataagaagac tatatatgac acaatttagc accaagttac cgttgccctt gattgtggaa 1140
atgaacagaa gaatggtcaa aggttacgaa ttctatagaa acgatcctaa aatagcggac 1200
ttgaccaaag atataatggc atataatgcc gccttgagac actataatct tcctgatcac 1260
cttgtggagg aggcaaaggt aaatttcgca aaaaacctcg gacttgtttt ttttagatcc 1320
atcgggctct gcatcctctt ttcgttagcc atgccaggta tcattatgtt ctcacctgtc 1380
ttcatattag ccaagagaat ttctcaagaa aaggcccgta ccgctttgtc caagtctaca 1440
gttaaaataa aggctaacga tgtcattgcc acgtggaaaa tcttgattgg gatgggattt 1500
gcgcccttgc tttacatctt ttggtccgtt ttaatcactt attacctcag acataaacca 1560
tggaataaaa tatatgtttt ttccgggtct tacatctcgt gtgttatagt cacgtattcc 1620
gccttaatcg tgggtgatat tggtatggat ggtttcaaat ctttgagacc actggtttta 1680
tctcttacat ctccaaaggg cttgcaaaag ctacaaaagg atcgtagaaa tctggcagaa 1740
agaataatcg aagttgtaaa taactttgga agcgaattat tccccgattt cgatagtgcc 1800
gccctacgtg aagaattcga cgtcatcgat gaagaggaag aagatcgaaa aacctcagaa 1860
ttgaatcgca ggaaaatgct aagaaaacag aaaataaaaa gacaagaaaa agattcgtca 1920
tcacctatca tcagccaacg tgacaaccac gatgcctatg aacaccataa ccaagattcc 1980
gatggcgtct cattggtcaa tagtgacaat tccctctcta acattccatt attctcttct 2040
acttttcatc gtaagtcaga gtcttcctta gcttcgacat ccgttgcacc ttcttcttcc 2100
tccgaatttg aggtagaaaa cgaaatcttg gaggaaaaaa atggattagc aagtaaaatc 2160
gcacaggccg tcttaaacaa gagaattggt gaaaatactg ccagggaaga ggaagaggaa 2220
gaagaagagg aagaagaaga agaggaagaa gaagaagaag ggaaagaagg agatgcgtag 2280
<210> 43
<211> 710
<212> PRT
<213> 噬腺嘌呤阿克苏拉酵母
<400> 43
Met Thr Ser Glu Ser Ile Asp Thr Gly Leu Asn Phe Lys Thr Trp Leu
1 5 10 15
Tyr Asp Leu Val Leu Trp Ile Phe Gln Val Thr Phe Asn Ile Phe Phe
20 25 30
Arg Glu Ile Leu Ser Arg Gly Ala Phe Arg Ile Pro Lys Ser Gly Pro
35 40 45
Val Ile Phe Val Gly Ala Pro His Ala Asn Gln Phe Val Asp Pro Met
50 55 60
Leu Leu Met Gln Gln Ala Lys Gly Val Ala Gly Arg Arg Leu Ser Phe
65 70 75 80
Leu Ile Ala Glu Thr Ser Leu Arg Arg Lys Phe Ile Gly Ala Ile Ala
85 90 95
Arg Ala Thr Gln Ser Ile Gly Val Val Arg Ala Gln Asp Asn Leu Lys
100 105 110
Pro Gly Ser Gly Lys Ile Ser Val Asp Ala Asp Asn Gly Thr Arg Ile
115 120 125
Ile Gly Glu Gly Thr Lys Phe Thr Thr Glu Cys Met Val Lys Gly Ile
130 135 140
Ile Gly Leu Pro Gln Gln Ala Gly Ser Ala Glu Ile Ala Glu Ile Val
145 150 155 160
Ser Asp Thr Glu Leu Ile Leu Arg Lys Glu Phe Lys Gly Ala Lys Ala
165 170 175
Ala Ser Leu Leu Lys Arg Gly Thr Ser Tyr Lys Arg Ala Asp His Val
180 185 190
Asp Gln Ser Lys Met Tyr Arg Gln Val Phe Asp His Leu His Lys Gly
195 200 205
Gly Cys Leu Gly Ile Phe Pro Glu Gly Gly Ser His Asp Arg Thr Asp
210 215 220
Leu Leu Pro Leu Lys Ala Gly Val Ala Ile Met Ala Leu Gly Ala Ile
225 230 235 240
Met Glu Tyr Pro Asp Leu Asp Val Lys Ile Val Pro Cys Gly Met Asn
245 250 255
Tyr Phe His Pro Asn Lys Phe Arg Ser Arg Ala Val Ile Glu Phe Gly
260 265 270
Ser Pro Met Thr Ile Pro Arg Glu Leu Val Glu Met Tyr Lys Gln Gly
275 280 285
Gly Glu Gln Lys Arg Asp Ala Val Lys Gln Leu Leu Asp Gln Val Ala
290 295 300
Asp Gly Leu Arg Thr Val Thr Val Thr Thr Pro Asp Tyr Glu Thr Leu
305 310 315 320
Met Val Val Gln Ala Ala Arg Arg Leu Tyr Arg Pro Pro Asn Lys Lys
325 330 335
Leu Pro Leu Pro Val Val Val Glu Leu Asn Arg Arg Leu Ile Arg Gly
340 345 350
Tyr Glu Lys Tyr Lys Asp Asp Pro Lys Ile Val His Leu Arg Asn Ala
355 360 365
Val Leu Ser Tyr Asn Lys Lys Leu Lys Gly Leu Gly Leu Lys Asp His
370 375 380
Gln Val Glu Thr Ala Thr Leu Ser Pro His Lys Val Val Phe Lys Phe
385 390 395 400
Leu Tyr Arg Leu Ser Lys Leu Leu Val Leu Ala Pro Leu Ala Met Pro
405 410 415
Gly Ala Ile Met Phe Ser Pro Val Phe Val Ala Thr Lys Leu Ile Ser
420 425 430
Arg Lys Arg Ala Lys Gln Ala Leu Ala Lys Ser Thr Val Lys Val Gln
435 440 445
Ala Arg Asp Val Val Ala Thr Trp Lys Val Leu Val Ala Met Gly Leu
450 455 460
Ala Pro Cys Leu Tyr Thr Val Tyr Ala Leu Val Ala Thr Tyr Ile Cys
465 470 475 480
Tyr Lys Lys Gln Trp Val Ser Ser Glu Leu Trp Ser Leu Ala Lys Val
485 490 495
Met Ile Ala Ser Tyr Ile Val Phe Pro Ala Ile Thr Trp Ser Ala Leu
500 505 510
Val Ile Gly Glu Thr Gly Met Asp Ile Phe Lys Ser Leu Arg Pro Leu
515 520 525
Ala Leu Ala Leu Asn Pro Phe His Lys Asn Ala Ile Glu Glu Leu Arg
530 535 540
Glu Thr Arg Arg Asn Leu Val Met Glu Val Ser Glu Val Val Asn Ser
545 550 555 560
Leu Gly Pro Glu Leu Tyr Pro Asp Phe Gly Lys Tyr Ser Phe Arg Tyr
565 570 575
Asn Glu Tyr Gln Tyr Lys Glu Glu Lys Leu Ala Asn Gly Lys Val Glu
580 585 590
Glu Ala Glu Glu Thr Glu Glu Ala Lys Glu Ala Lys Glu Asp Glu Glu
595 600 605
Glu Asn Val Val Val Ala Lys Arg Arg Thr Ser Ser Ala Ser Thr Asp
610 615 620
Thr Asn Val Ser Ser Asp Ser Asn Ser Ile Ser Arg Val Asn Ser Glu
625 630 635 640
Ser Gly Leu Ala Asn Ile Pro Leu Phe Ser Ser Ala Asp Pro Val Ser
645 650 655
Asn His Ser Arg Ala Ser Ser Gly Ser Ser Ala Leu Ser Met Glu Val
660 665 670
Pro Asp Thr Thr Thr Ala Thr Gly Gln Lys Val Phe Gln Ser Glu Val
675 680 685
Ser Lys Arg Ile Arg Gly Ala Met Glu Glu Arg Ile Arg Ala Arg Met
690 695 700
Glu Glu Ser Asp Glu Glu
705 710
<210> 44
<211> 2133
<212> DNA
<213> 噬腺嘌呤阿克苏拉酵母
<400> 44
atgactagcg aaagcataga cacaggacta aatttcaaga catggctcta cgatctagtg 60
ctatggatct ttcaggtgac atttaacatc ttcttccgag agattctttc tcgaggagca 120
ttcaggatcc caaagtccgg ccccgtcatc tttgtaggag cacctcatgc taaccagttt 180
gtagatccaa tgctgctgat gcagcaggcc aagggagttg cgggccgtcg tctttcgttt 240
ttgattgctg aaacgtcgtt gcgacgcaag tttattggcg caattgcccg ggccacccag 300
tcaattggag tggtacgtgc tcaggacaat ttgaagcctg ggtctggtaa gatttcagta 360
gatgccgaca atgggacccg tatcattggt gagggaacaa agttcaccac tgagtgtatg 420
gtcaagggta taattggatt gcctcagcaa gcgggcagcg cagagattgc tgaaattgtc 480
agcgacactg agctgattct gcgaaaggag tttaagggcg caaaggctgc ttctctgctt 540
aagcgaggaa cttcatacaa gagagcagac catgtggatc agtcgaaaat gtaccgtcaa 600
gtgtttgacc atcttcacaa gggtggatgc cttggtatct ttcccgaagg aggatctcat 660
gaccgtactg acctgttgcc tttgaaagct ggtgtagcga ttatggctct gggggccatt 720
atggagtatc ctgacctgga tgtgaaaatt gttccctgtg gtatgaacta tttccatcct 780
aacaagttta gatcccgagc agtcattgag tttggaagtc ccatgaccat tccccgggag 840
ttagtagaaa tgtacaaaca aggaggtgaa cagaaacgag atgcagttaa gcaattgctt 900
gaccaggttg ctgatgggct gagaacagtt actgtaacca ctcctgatta cgagactctg 960
atggttgttc aagcagcccg acgattgtac cgccctccta acaagaagct gccgttgcca 1020
gtggttgtgg aactgaaccg ccgactgatt cgtggatatg aaaagtacaa ggacgacccc 1080
aagattgttc atctcaggaa tgcagtcttg tcgtacaaca aaaaactcaa gggactgggc 1140
ctcaaggatc accaagtaga aactgccacc ctctctcctc acaaggttgt attcaagttt 1200
ttgtatcgac tttcaaagct gctggttctg gcccctctgg ctatgcctgg tgccatcatg 1260
ttctctcccg tgtttgtggc taccaagctg atctctagga agcgggccaa acaggcactt 1320
gccaagtcta ctgtcaaagt gcaggctcgc gatgtggtag ccacctggaa ggtacttgtt 1380
gccatgggat tagcaccatg cttgtatacc gtttacgctc tagtggccac ttacatttgc 1440
tacaagaagc aatgggtatc ctcagagctg tggtcgttgg ctaaggtaat gattgcttcg 1500
tacattgttt tccctgctat tacctggtct gctttggtga ttggtgagac cggtatggac 1560
attttcaagt ccctgcgccc tctggcgttg gcgctcaacc cattccacaa gaatgctatt 1620
gaagagctcc gagaaactcg acgcaatttg gtcatggaag tttctgaagt tgtcaattcc 1680
ctcgggcctg agctgtaccc agactttggc aagtactctt tccgatacaa cgaatatcag 1740
tacaaggagg agaaactggc caacggcaaa gtcgaagagg ccgaagagac cgaagaggcc 1800
aaagaggcca aagaggacga agaagaaaat gtggttgtgg ccaaacgacg cacgagcagt 1860
gcgtctacag atactaacgt ttcatcggat tccaattcca tctctcgagt caactcggag 1920
tctggcctgg ccaatattcc tctgttttcc agcgctgatc cagtaagcaa ccactctcga 1980
gccagttccg gttcgtctgc gttgagcatg gaagtgccag ataccaccac tgccactgga 2040
cagaaggtgt tccagtctga ggtatccaag cgaattcggg gtgccatgga ggagagaatc 2100
cgagctcgaa tggaagagag cgatgaggag tag 2133
<210> 45
<211> 906
<212> PRT
<213> 粘红酵母
<400> 45
Met Pro Ala Ser Pro Asp Pro Ser Ser Ser Ser Asn Asp Val Asp Ser
1 5 10 15
Ala Pro Pro Asp Ser Gln Pro Pro Leu Pro Ser Ser Gly Ser Ala Leu
20 25 30
Gly Leu Ser Phe Ala Pro Glu Asp Ser Pro Ala Pro Ala Pro Thr Pro
35 40 45
Ala Gln Ala Pro Gly Ala Pro Leu Pro Glu Leu Ser Pro Ile Pro Ala
50 55 60
Asp Pro Ala Pro Arg Leu Ala Lys Gln Ala His Phe Ser Asp Leu Thr
65 70 75 80
Gln Ile Arg Thr Ile Ser Pro Val Pro Gln Arg Arg Gln Ser Tyr Arg
85 90 95
Gln Pro Ser Thr Pro Ala Glu Pro Ala Pro Ala Pro Pro Pro Gly Asp
100 105 110
Ser Leu Pro Arg Arg Pro Ser Leu Thr Gln Thr His Ser Asp Leu Arg
115 120 125
Thr Leu Leu Lys Asn Phe Leu Leu Leu Val Pro Pro Ala Leu Arg Arg
130 135 140
Leu Arg Phe Leu Val Pro Ser Pro Leu Arg Val Ile Ala Arg Phe Val
145 150 155 160
Ile Arg Tyr Val Ala Met Phe Leu His Ala Arg Gly Ala Leu Gly Ser
165 170 175
Asn Leu Val Tyr Asp Val Val Ala His Met Trp Arg Val Val Ile Thr
180 185 190
Ile Phe Phe Arg Glu Ile Arg Ser Arg Gly Ala Trp Lys Ile Pro Arg
195 200 205
Ser Ser Glu Gly Ala Val Ile Phe Val Val Gly Pro His His Asn Gln
210 215 220
Phe Leu Asp Pro Leu Leu Leu Met Ser Glu Val Lys Arg Glu Ser Gly
225 230 235 240
Arg Arg Ile Ser Phe Leu Ala Ala Ala Lys Ser Met Asp Arg Ala Phe
245 250 255
Val Gly Leu Ala Ser Arg Leu Met Gln Ser Ile Pro Val Ala Arg Ala
260 265 270
Gln Asp Tyr Ala Phe Ala Gly Gln Gly Thr Ile Ser Leu Ser Pro Ser
275 280 285
Asp Pro Leu Thr Ile Leu Gly Thr Gly Thr Asn Phe Thr Lys Asp Phe
290 295 300
Ser Lys Pro Arg Ser Gln Leu Leu Leu Pro Arg Asn Leu Gly Ser Ser
305 310 315 320
Thr Ala Glu Val Val Glu Val Val Ser Asp Thr Glu Leu Lys Leu Lys
325 330 335
Lys Glu Phe Ser Lys Lys Ala Leu Asp Ala Leu Lys Glu Arg Glu Asp
340 345 350
Gly Val Ala Phe Lys Val Leu Pro His Val Asp Gln Ser Ser Met Tyr
355 360 365
Ser Ala Val Tyr Gln Lys Leu Ile Asp Gly Gly Cys Ile Gly Ile Phe
370 375 380
Pro Glu Gly Gly Ser His Asp Arg Thr Asp Leu Leu Pro Leu Lys Ala
385 390 395 400
Gly Val Ser Ile Met Ala Leu Gly Ala Lys Ser Ala His Pro Asp Leu
405 410 415
Lys Leu Gln Ile Val Pro Val Gly Leu Ser Tyr Phe His Pro His Lys
420 425 430
Phe Arg Ser Arg Ala Val Val Glu Phe Gly Ser Pro Ile Glu Ile Pro
435 440 445
Gln Asp Tyr Val Ser Glu Phe Glu Lys Gly Gly Glu Asn Lys Lys Lys
450 455 460
Ala Ile Gly Glu Val Met Glu Leu Ile Val Asp Gly Leu Lys Ser Val
465 470 475 480
Thr Ile Arg Ala Pro Asp Tyr Glu Thr Leu Met Leu Ile Gln Ala Ala
485 490 495
Arg Arg Leu Tyr Arg Pro Pro Gly Thr Asn Leu Thr Ile Gly Gln Val
500 505 510
Val Glu Leu Asn Lys Arg Phe Ile Val Gly Tyr Glu Val Tyr Lys Asp
515 520 525
Asp Pro Arg Ile Lys Glu Leu Glu Arg Gly Val Arg Glu Tyr Asn Thr
530 535 540
Leu Leu Arg Tyr Met Gly Leu Lys Asp His Gln Val Glu Ser Val Gly
545 550 555 560
Arg Pro Arg Trp Arg Ser Phe Phe Leu Leu Cys Tyr Arg Leu Gly Leu
565 570 575
Leu Ser Val Trp Gly Val Leu Ala Leu Pro Gly Val Val Leu Asn Ala
580 585 590
Pro Ile Phe Ile Ala Ala Lys Leu Ile Ser Arg Ala Lys Ala Lys Glu
595 600 605
Ala Leu Ala Ala Ser Thr Val Lys Ile Ala Gly Arg Asp Val Leu Ala
610 615 620
Thr Trp Lys Val Leu Val Ala Leu Ala Gly Ala Pro Ser Leu Tyr Thr
625 630 635 640
Ile Tyr Ala Ile Asn Ala Val Val Leu Ala His Lys Leu Gly Leu Pro
645 650 655
Tyr Lys Tyr Lys Leu Ala Ala Pro Phe Ala Thr Phe Ala Gly Leu Pro
660 665 670
Phe Ile Gly Val Ala Ala Leu Lys Phe Gly Glu Val Gly Met Asp Val
675 680 685
Tyr Lys Ser Met Arg Pro Leu Leu Leu Ser Leu Ile Pro Gly Lys Glu
690 695 700
Pro Glu Leu Lys Arg Leu Arg His Met Arg Glu Thr Leu Ala Ser Glu
705 710 715 720
Leu Asn Glu Leu Val Asp Glu Leu Ala Pro Thr Val Phe Glu Asp Phe
725 730 735
Asp Ser Arg Arg Ile Ile Pro Ser Thr Asp Val Gly Val Arg Arg Glu
740 745 750
Ser Ala Gln Gly Lys Phe Leu Gln His Pro Leu Asn Trp Val Asp Glu
755 760 765
Leu Leu Phe Gly Ser Gly Trp Ser Gln Ser Met Ala His Pro Ala Asp
770 775 780
Arg Lys Val Lys Ser Met Leu Pro Glu Thr Ser Gly Met Glu Ser Asp
785 790 795 800
Met Asp Gly Gly Phe Thr Asp Gly Gln Gly Gly Gly Ser Gly Tyr Ala
805 810 815
Ser Gly Tyr Thr Thr Glu Asp Ala Pro Asp Tyr Asp Glu Val Ile His
820 825 830
Ile Leu Asn Arg Glu Gln Gly Arg Pro Asp Ser Pro Leu Pro Ser Pro
835 840 845
Arg Pro Gly Leu Tyr Arg Arg Ala Ser Arg Gln Arg Ser Arg Ser Gln
850 855 860
Leu Asn Leu Ala Gly Met Ser Pro Val Thr Pro Thr Thr Pro Leu Ala
865 870 875 880
Ala Ser Thr Ser Leu Gln Asp Gly Gly Glu Gly Thr Ala Arg Arg Arg
885 890 895
Thr Arg Gln Gly Ser Gly Asp Ala Gln Glu
900 905
<210> 46
<211> 2721
<212> DNA
<213> 粘红酵母
<400> 46
atgcccgcct cgcccgaccc ctcctcctca tccaacgacg tcgacagcgc tcctcccgac 60
tcgcaaccgc ctctcccctc gtctggatct gcgctcgggc tctcgttcgc gccagaagac 120
tcgcctgcac ccgcgccaac tcccgcacag gctccaggcg cgccgttgcc agagctctcc 180
cccatccctg ccgatcctgc tcctcgactt gcgaaacagg cgcacttcag cgacttgacg 240
cagatacgca ccatcagccc agtcccacag cgcaggcagt cctaccgcca gccgtccacg 300
cccgcagagc cagcgcccgc accaccgccc ggcgactctc tccctcgacg gccgtcgctc 360
acgcagacgc actcggacct gcgcaccttg ctcaagaact tcctcctcct cgtcccgccc 420
gcactccgcc gcctgcgctt cctcgtcccc tccccgctcc gcgtcatcgc ccgcttcgtc 480
attcgatacg tcgcaatgtt cctccacgcc cgcggcgcac tcggcagcaa cctcgtctac 540
gatgtcgtcg cgcacatgtg gcgagtcgtc atcaccatct tcttccgcga gatccgttct 600
cgcggcgcgt ggaagatccc gaggtcgtcg gagggcgccg tcatcttcgt tgttgggcct 660
caccacaacc agttcctcga ccccctcctg cttatgagtg aggtcaagcg cgagagcgga 720
cggcggatca gcttcctggc ggcggcaaag agcatggaca gggcattcgt cggcttggcg 780
tcgcggttga tgcagagcat ccccgtcgcc cgcgctcaag actacgcctt tgcaggccaa 840
ggcacaatct ccctctcccc ctccgaccca ctcaccatcc tcggcaccgg taccaacttc 900
accaaagact tctcgaaacc tcgaagccag ctccttctcc ctcgcaacct tggaagcagc 960
acggccgagg tcgtcgaagt cgttagcgat acggagttga agctcaagaa ggagttttcg 1020
aagaaggcgc ttgatgcctt gaaggagagg gaggacggcg tcgcgttcaa ggtcctcccg 1080
cacgtcgacc agtcgagcat gtacagcgcc gtttaccaga agctcatcga cggcggctgc 1140
atcggcatct tccccgaagg cggctctcac gaccgtaccg acctcctccc cctcaaagcc 1200
ggcgtctcga tcatggcgct cggcgcaaag tcggcccacc cagacctcaa gctccagatt 1260
gtccccgtcg gattgagcta cttccaccct cacaagttcc gctcgcgcgc tgtcgtcgag 1320
tttggaagcc cgattgagat cccgcaggac tatgtcagcg agtttgagaa gggcggcgag 1380
aacaagaaga aggccattgg ggaggtgatg gagttgattg tcgacgggct caagagcgtc 1440
acgattcgcg cgcccgacta cgagacgttg atgctcatcc aagccgcccg tcgcctgtac 1500
cgccctcccg gtacgaacct cacgatcggc caagtcgtcg agctcaacaa gcgcttcatc 1560
gtcggctacg aagtgtacaa ggacgacccg cgaatcaagg agctcgagcg cggcgtgcgc 1620
gagtacaaca cgttgttgcg gtacatgggc ctcaaggatc accaggttga aagcgtggga 1680
aggccgaggt ggaggtcgtt cttcctcctc tgctacaggc ttgggttgtt gagcgtctgg 1740
ggcgtcctcg ccctgccggg agtcgtcctc aacgccccga tcttcatcgc cgccaagctc 1800
atctcgcggg cgaaggccaa agaggctctc gccgcctcga cagtcaagat cgccggccgc 1860
gacgtcctcg caacctggaa ggtcctcgtc gcgctcgccg gcgcaccctc gctctacaca 1920
atctacgcca tcaacgccgt cgtcctcgcg cacaagctcg gcttgccgta caagtacaag 1980
cttgcggcgc cgtttgcgac gtttgcggga ttgccgttca tcggtgttgc ggcgctcaag 2040
ttcggcgagg tcggcatgga cgtctacaag tcaatgcgcc cgctcctcct ctctctgatc 2100
cccggcaagg agcccgagct gaagcgcctg cgacacatgc gcgagacgct cgcctcggag 2160
ctgaacgagc tcgtcgacga gctcgcgcca accgtcttcg aagacttcga ctctcgccgc 2220
atcatcccct cgacggacgt cggcgtccgc cgcgagtcgg cgcagggcaa gttcctccag 2280
cacccgctca actgggtcga cgagttgttg tttggatcgg ggtggagcca gtcgatggcg 2340
catccggcgg acaggaaggt caagagcatg ctgcccgaga ccagcgggat ggagagcgat 2400
atggacggcg ggttcaccga tggtcagggc ggcggcagcg ggtacgcctc gggctatacg 2460
accgaggatg cgcccgacta cgacgaggtc atccacatcc tcaatcgcga gcaaggccgt 2520
cccgactcgc ctctcccttc ccctcgtcca ggtttgtacc gccgagcatc ccgccagcgc 2580
tcgcgctcgc agctcaacct cgcgggcatg agccccgtca cgccgacgac tcctttggcg 2640
gcttcgacga gcttgcagga cgggggcgag ggcacggcga ggcggaggac gaggcagggt 2700
tcgggtgatg cgcaggagtg a 2721
<210> 47
<211> 907
<212> PRT
<213> 圆红冬孢酵母
<400> 47
Met Pro Ala Ser Pro Asp Ser Ser Ser Ser Ser Ser Asn Asp Val Asp
1 5 10 15
Thr Ala Pro Pro Asp Ser Arg Pro Pro Leu Pro Pro Pro Gly Ser Ala
20 25 30
Leu Gly Leu Ser Phe Ala Pro Glu Asp Ser Pro Val Ser Ala Gln Thr
35 40 45
Pro Ala Gln Ala Pro Gly Ala Pro Val Pro Glu Leu Ser Pro Ile Pro
50 55 60
Ala Asp Pro Ala Pro Arg Leu Ala Lys Gln Ala His Phe Ser Asp Leu
65 70 75 80
Thr Gln Ile Arg Thr Ile Ser Pro Val Pro Gln Arg Arg Gln Ser Tyr
85 90 95
Arg Gln Pro Ser Thr Pro Ala Glu Pro Ala Pro Ala Pro Pro Pro Arg
100 105 110
Asp Ser Pro Pro Arg Arg Pro Ser Leu Thr Gln Thr His Ser Asp Leu
115 120 125
Arg Thr Leu Leu Lys Ser Phe Leu Leu Leu Val Pro Pro Ala Leu Arg
130 135 140
Arg Leu Arg Phe Leu Val Pro Ser Pro Leu Arg Val Ile Ala Arg Phe
145 150 155 160
Val Ile Arg Tyr Val Ala Met Phe Leu His Ala Arg Gly Ala Leu Gly
165 170 175
Ser Asn Leu Val Tyr Asp Val Val Ala His Met Trp Arg Val Val Ile
180 185 190
Thr Ile Phe Phe Arg Glu Ile Arg Ser Arg Gly Ala Trp Lys Ile Pro
195 200 205
Arg Ser Ser Glu Gly Ala Val Ile Phe Val Val Gly Pro His His Asn
210 215 220
Gln Phe Leu Asp Pro Leu Leu Leu Met Ser Glu Val Lys Arg Glu Ser
225 230 235 240
Gly Arg Arg Ile Ser Phe Leu Ala Ala Ala Lys Ser Met Asp Arg Ala
245 250 255
Phe Val Gly Leu Ala Ser Arg Leu Met Gln Ser Ile Pro Val Ala Arg
260 265 270
Ala Gln Asp Tyr Ala Phe Ala Gly Gln Gly Thr Ile Ser Leu Ser Pro
275 280 285
Ser Asp Pro Leu Thr Ile Leu Gly Thr Gly Thr Asn Phe Thr Lys Asp
290 295 300
Phe Ser Lys Pro Arg Ser Gln Leu Leu Leu Pro Arg Asn Leu Gly Ser
305 310 315 320
Ser Thr Ala Glu Val Ile Glu Val Val Ser Asp Thr Glu Leu Lys Leu
325 330 335
Lys Lys Glu Phe Ser Lys Lys Ala Leu Asp Ala Leu Lys Glu Arg Glu
340 345 350
Asp Gly Val Ala Phe Lys Val Leu Pro His Val Asp Gln Ser Ser Met
355 360 365
Tyr Ser Ala Val Tyr Gln Lys Leu Ile Asp Gly Gly Cys Ile Gly Ile
370 375 380
Phe Pro Glu Gly Gly Ser His Asp Arg Thr Asp Leu Leu Pro Leu Lys
385 390 395 400
Ala Gly Val Ser Ile Met Ala Leu Gly Ala Lys Ser Ala His Leu Asp
405 410 415
Leu Lys Leu Gln Ile Val Pro Val Gly Leu Ser Tyr Phe His Pro His
420 425 430
Lys Phe Arg Ser Arg Ala Val Val Glu Phe Gly Ser Pro Ile Glu Ile
435 440 445
Pro Gln Glu Tyr Val Ser Glu Phe Glu Lys Gly Gly Glu Asn Lys Lys
450 455 460
Lys Ala Ile Gly Glu Val Met Glu Leu Ile Val Asp Gly Leu Lys Ser
465 470 475 480
Val Thr Val Arg Ala Pro Asp Tyr Asp Thr Leu Met Leu Ile Gln Ala
485 490 495
Ala Arg Arg Leu Tyr Arg Pro Pro Gly Thr Asn Leu Thr Ile Gly Gln
500 505 510
Val Val Glu Leu Asn Lys Arg Phe Ile Val Gly Tyr Glu Val Tyr Lys
515 520 525
Asp Asp Pro Arg Ile Lys Glu Leu Glu Arg Gly Val Arg Glu Tyr Asn
530 535 540
Thr Leu Leu Arg Tyr Met Gly Leu Lys Asp His Gln Val Glu Ser Val
545 550 555 560
Gly Arg Pro Arg Trp Arg Ser Phe Phe Leu Leu Cys Tyr Arg Leu Gly
565 570 575
Leu Leu Ser Val Trp Gly Val Leu Ala Leu Pro Gly Val Val Leu Asn
580 585 590
Ala Pro Ile Phe Ile Ala Ala Lys Leu Ile Ser Arg Ala Lys Ala Lys
595 600 605
Glu Ala Leu Ala Ala Ser Thr Val Lys Ile Ala Gly Arg Asp Val Leu
610 615 620
Ala Thr Trp Lys Val Leu Val Ala Leu Ala Gly Ala Pro Ser Leu Tyr
625 630 635 640
Thr Ile Tyr Ala Ile Asn Ala Val Val Leu Ala His Lys Leu Gly Leu
645 650 655
Pro Tyr Lys Tyr Lys Leu Ala Ala Pro Phe Ala Thr Phe Ala Gly Leu
660 665 670
Pro Val Ile Gly Val Ala Ala Leu Lys Phe Gly Glu Val Gly Met Asp
675 680 685
Val Tyr Lys Ser Met Arg Pro Leu Leu Leu Ser Leu Ile Pro Gly Lys
690 695 700
Glu Pro Glu Leu Lys Arg Leu Arg His Met Arg Glu Thr Leu Ala Ser
705 710 715 720
Glu Leu Asn Glu Leu Val Asp Glu Leu Ala Pro Thr Val Phe Glu Asp
725 730 735
Phe Asp Ser Arg Arg Ile Ile Pro Ser Thr Asp Val Gly Val Arg Arg
740 745 750
Glu Ser Ala Gln Gly Lys Phe Leu Gln His Pro Leu Asn Trp Val Asp
755 760 765
Glu Leu Leu Phe Gly Ser Gly Trp Ser Gln Ser Met Ala His Pro Ala
770 775 780
Asp Arg Lys Val Lys Ser Met Leu Pro Glu Thr Ser Gly Met Glu Ser
785 790 795 800
Asp Met Asp Gly Gly Phe Thr Asp Gly Gln Gly Gly Gly Ser Gly Tyr
805 810 815
Ala Ser Gly Tyr Thr Thr Glu Asp Ala Pro Asp Tyr Asp Glu Val Ile
820 825 830
His Ile Leu Asn Arg Glu Gln Gly Arg Pro Asp Ser Pro Leu Pro Ser
835 840 845
Pro Arg Pro Gly Leu Tyr Arg Arg Val Ser Arg Gln Arg Ser Arg Ser
850 855 860
Gln Leu Asn Leu Ala Gly Met Ser Pro Val Thr Pro Thr Thr Pro Leu
865 870 875 880
Ala Ala Ser Thr Ser Leu Gln Asp Gly Gly Glu Gly Thr Ala Arg Arg
885 890 895
Arg Thr Arg Gln Gly Ser Gly Asp Ala Gln Glu
900 905
<210> 48
<211> 2724
<212> DNA
<213> 圆红冬孢酵母
<400> 48
atgcccgcct cgcccgactc gtcctcctcc tcatccaacg acgtcgacac cgctcctccc 60
gactcgcgac cgcctctccc tccgcctggc tctgcgttgg ggctctcctt tgcgccagaa 120
gactcgcctg tatccgcgca aactcccgca caggctccgg gcgcgccggt gccagagctc 180
tcccccatcc ctgccgatcc cgctcctcga cttgcgaaac aggcgcactt cagcgacctg 240
acgcagattc gcaccatcag cccagtacca cagcgcaggc agtcctaccg ccaaccgtcc 300
acgcccgcag agccagcgcc cgccccgccg ccccgcgact ctccccctcg acgaccgtcg 360
ctcacgcaga cgcactcgga cctgcgcacg ctgctcaaga gtttcctcct cctcgtcccg 420
cccgcactcc gccgcctgcg cttcctcgtc ccctctccgc tccgcgtcat cgcccgcttc 480
gtcattcgat acgtcgcaat gttcctccac gctcgcggcg cgctcggcag caacctcgtc 540
tacgatgtcg tcgcgcacat gtggcgagtc gtcatcacca tcttcttccg cgagatccgc 600
tcccgcggcg cgtggaagat cccgaggtcg tcggagggag ccgtcatctt cgttgttggg 660
cctcaccaca accagttcct cgaccctctc ctgcttatga gcgaggtcaa gcgcgagagc 720
ggacgacgga tcagcttcct ggccgcggca aagagcatgg acagggcgtt cgttggcttg 780
gcgtcgcgct tgatgcaaag cattcccgtc gcccgcgcgc aagactacgc ctttgcaggt 840
caaggcacca tctcgctctc cccctcagac ccactcacca tcctcggcac cggcaccaac 900
ttcaccaaag acttctcgaa accccgaagc cagctcctcc tccctcgcaa cctcggcagc 960
agcacggccg aggtcatcga ggtcgtcagt gatacggagt tgaagctcaa aaaggagttt 1020
tcgaagaagg cgcttgatgc gttgaaggag cgggaggacg gcgttgcgtt caaggtcctc 1080
ccgcacgtcg accagtcgag catgtacagc gccgtctacc aaaagctcat cgacggcggc 1140
tgcatcggca ttttccccga aggcggctct cacgaccgta ccgacctcct cccactcaaa 1200
gccggcgtct ccatcatggc gcttggcgcg aagtcggccc acctagacct caagctccag 1260
atcgtccccg tcggactgag ctacttccac cctcacaagt tccgctcgcg cgctgtcgtc 1320
gagtttggga gcccgattga gataccgcag gaatatgtca gcgagtttga gaagggcggc 1380
gagaacaaga agaaggccat tggggaggtg atggagctga ttgtcgacgg gctcaagagc 1440
gtcacggttc gcgcgcctga ctacgatacg ttgatgctca tccaagccgc tcgccgcctg 1500
taccgccctc ccggcacgaa cctcacgatt ggccaagtcg tcgagctcaa caagcgcttc 1560
atcgtcgggt acgaagtgta caaggacgac ccgcgaatca aggagctcga gcggggcgtg 1620
cgcgagtaca acacgctgtt gcggtacatg ggcctcaagg atcaccaggt tgagagcgtg 1680
ggaaggccca ggtggagatc gttcttcctc ctttgctaca ggcttgggtt gttgagcgtc 1740
tggggcgtcc tggcgctgcc cggagtcgtc cttaacgcgc cgatcttcat cgccgccaag 1800
ctcatctcgc gggcaaaggc caaggaggct ctcgccgcct cgaccgtcaa gatcgccggc 1860
cgcgacgtcc tcgcaacctg gaaggtcctc gtcgcgctcg ctggcgcacc atcgctctac 1920
acgatctacg ccatcaacgc cgtcgtcctc gcgcacaaac ttggcttgcc gtacaagtac 1980
aagcttgcgg cgccctttgc gacgtttgcg gggttgccgg tcatcggtgt tgcggcgctc 2040
aagtttggcg aggtcggcat ggacgtctac aagtcaatgc gcccgctcct cctctcgctc 2100
atccccggca aggagcccga gctgaagcgc ctgcgacaca tgcgcgagac gctcgcctcg 2160
gagctgaacg agctcgtcga cgagctcgcg ccgaccgtct tcgaagactt cgactctcgc 2220
cgcatcattc cctcgacgga cgtcggcgtc cgccgcgagt cggcgcaggg caagttcctc 2280
cagcacccgc tcaactgggt cgacgagttg ttgttcggat cggggtggag ccagtcgatg 2340
gcgcacccgg cggaccggaa ggtcaagagc atgctgcccg agacgagcgg gatggagagc 2400
gatatggacg gcgggttcac cgatggtcag ggtggcggca gcgggtacgc ctcgggctac 2460
acgaccgagg atgcgcccga ctacgacgaa gtcatccaca tcctcaaccg cgagcaaggt 2520
cgtcccgact ctcctctccc ttcacctcgc cccggcttgt accgccgcgt ctcccggcag 2580
cgttcgcgct cgcagctcaa cctggcgggg atgagccccg tcacgccgac aactcccttg 2640
gcggcttcga cgagcttgca ggacggcggt gagggtacgg cgaggcggag gacgagacaa 2700
ggctcgggcg acgcgcagga gtga 2724
<210> 49
<211> 413
<212> PRT
<213> 噬腺嘌呤阿克苏拉酵母
<400> 49
Met Ser Val Thr Leu Thr Gln Arg Arg Pro Ser Met Thr Ala Pro Lys
1 5 10 15
Glu Glu Pro Lys Lys Ala Leu Asp Thr Phe Gly Asn Glu Phe Val Val
20 25 30
Pro Ser Tyr Ser Ile Lys Asp Ile Leu Asp Ala Ile Pro Ala His Cys
35 40 45
Tyr Lys Arg Ser Ala Val Arg Ser Leu Ser Tyr Val Ala Arg Asp Val
50 55 60
Phe Phe Ile Gly Leu Phe Gly Tyr Leu Ala Ala Ser Tyr Ile His Leu
65 70 75 80
Ile Pro Trp Met Ser Gly Arg Val Val Ala Trp Phe Leu Tyr Ala Phe
85 90 95
Val Gln Gly Leu Phe Gly Thr Gly Cys Trp Val Leu Ala His Glu Cys
100 105 110
Gly His Arg Ala Phe Ser Glu Ser Asn Ala Ile Asn Asp Ser Val Gly
115 120 125
Trp Val Leu His Ser Ala Leu Leu Val Pro Tyr His Ser Trp Arg Leu
130 135 140
Ser His Ser Lys His His Lys Ala Thr Gly His Met Thr Arg Asp Met
145 150 155 160
Val Phe Val Pro Lys Thr Arg Asp Glu Phe Leu Glu Arg Arg Gly Val
165 170 175
Glu Pro Gly Thr Lys Ile Thr Asp Asn Leu Glu Asp Ala Pro Ile Val
180 185 190
Thr Leu Tyr Tyr Val Val Leu Gln Gln Leu Phe Gly Trp Ile Met Tyr
195 200 205
Leu Phe Thr Asn Val Thr Gly Gln Lys Tyr Pro Asn Arg Ser Lys Trp
210 215 220
Val Thr Asn His Phe Val Pro Thr Ser Pro Leu Tyr Asp Lys Lys Asp
225 230 235 240
Phe Ile Asn Ile Val Ile Ser Asp Ile Gly Ile Ile Ala Thr Leu Thr
245 250 255
Cys Leu Tyr Leu Ala Ser Gln Lys Trp Gly Phe Ser Thr Val Ala Leu
260 265 270
Met Tyr Val Phe Pro Tyr Leu Trp Val Asn His Trp Leu Val His Ile
275 280 285
Thr Phe Leu Gln His Thr Asp Pro Arg Leu Pro His Tyr Asn Ala Asp
290 295 300
Glu Trp Thr Phe Ala Lys Gly Ala Ser Ala Thr Ile Asp Arg Asp Phe
305 310 315 320
Gly Phe Ile Gly Arg His Ile Phe His Asp Ile Ile Glu Thr His Val
325 330 335
Leu His His Phe Val Ser Arg Ile Pro Phe Tyr Asn Gly Arg Glu Ala
340 345 350
Thr Glu Ala Ile Arg Lys Val Met Gly Thr His Tyr Gln Arg Asp Asp
355 360 365
Ser Asn Phe Val Thr Ser Leu Tyr Arg Val Ala Arg Thr Cys Gln Phe
370 375 380
Val Glu Gly Asp Asn Gly Val Ser Met Phe Arg Asn Val Asn Asn Ile
385 390 395 400
Gly Val Arg Pro Asn Leu Asp Gln Gly Gln Lys Leu Gln
405 410
<210> 50
<211> 1242
<212> DNA
<213> 噬腺嘌呤阿克苏拉酵母
<400> 50
atgtctgtca cccttactca acgtcgtccg tctatgacgg ctcccaagga ggagcccaag 60
aaggccttgg acacgtttgg aaatgaattc gttgtgcctt cgtactctat caaggacatt 120
ttggatgcca ttcccgctca ctgctacaag cgatccgcgg tgcgatctct gagctacgtg 180
gctcgggatg tcttcttcat tggtctgttc ggataccttg ctgcttcgta catccacctc 240
attccctgga tgtccggacg agtggttgcc tggttcctgt acgctttcgt acagggtctg 300
tttggaactg gatgctgggt cttggcccac gagtgtggac accgtgcctt ttctgaatcc 360
aatgctatca atgactctgt aggatgggtc cttcactccg ccctgctggt cccttatcac 420
tcgtggcgtc ttagtcactc caagcaccac aaggccactg gacacatgac ccgagacatg 480
gtgtttgtac caaagacccg agatgagttc cttgagcgcc gaggagtcga gcctggcacc 540
aagattaccg acaacctgga ggatgccccc attgtgaccc tgtactacgt tgtgctccag 600
cagctgtttg gatggattat gtatctgttc accaacgtta cgggacaaaa gtaccctaac 660
cgatcaaagt gggtgaccaa tcactttgtg cctacttctc ctctgtacga caagaaggac 720
tttatcaaca ttgtcatctc tgacattggt atcattgcta ccctgacctg tctgtacctt 780
gcttcccaga aatggggttt ctccaccgtg gccctcatgt acgttttccc ttacttgtgg 840
gttaaccact ggctggttca catcaccttc cttcagcaca ctgatccccg actccctcac 900
tacaatgccg acgaatggac ctttgccaag ggtgccagtg ctaccattga ccgagacttt 960
ggattcattg gccgtcacat cttccacgac attattgaga ctcacgttct tcaccacttt 1020
gtgtcgcgaa ttcctttcta caatggacga gaggccaccg aggcaatcag gaaggttatg 1080
ggcacgcact accagcgcga tgactccaac tttgttactt ccttgtaccg tgtcgctcga 1140
acttgtcaat ttgtggaagg agacaatggt gttagcatgt tccgcaatgt taacaacatt 1200
ggagtccggc ccaatttgga ccaagggcag aagcttcaat ag 1242
<210> 51
<211> 388
<212> PRT
<213> 噬腺嘌呤阿克苏拉酵母
<400> 51
Met Val Arg Phe Ala Pro Leu Asn Val Pro Leu His Arg Arg Leu Glu
1 5 10 15
Thr Phe Ala Leu Thr Tyr His Ile Leu Ser Ile Pro Val Trp Met Ser
20 25 30
Phe Phe Leu Leu Cys Cys Ala Ile Pro Leu Met Trp Pro Leu Val Ile
35 40 45
Ile Tyr Leu Leu Tyr Tyr Ala Ser Asp Asn Ser Ser Glu Asn Gly Gly
50 55 60
Val Ala Ser Arg Tyr Ser Pro Lys Phe Arg Ser Val Pro Leu Trp Lys
65 70 75 80
Tyr Phe Ala Asn Tyr Phe Pro Ile Thr Leu His Arg Thr Gln Glu Leu
85 90 95
Pro Pro Ala Phe Val Tyr Gln Gly Glu Asp Leu Asp Pro Glu Thr Pro
100 105 110
Asp Asp Ser Asp Asp Gly His Ala Lys Ser Lys Ser Ile Val Leu Lys
115 120 125
Leu Trp Lys Val Ala Phe Trp Trp Tyr Tyr Leu Pro Lys His Phe Leu
130 135 140
Arg Lys Pro Glu Val Arg Pro Thr Gly Arg Arg Tyr Ile Phe Gly Tyr
145 150 155 160
His Pro His Gly Ile Ile Gly Met Gly Ala Ile Gly Ala Ile Ala Thr
165 170 175
Glu Gly Ala Gly Trp Ser Lys Leu Phe Pro Gly Ile Pro Val Ser Leu
180 185 190
Leu Thr Leu Ala Asn Asn Phe Arg Ile Pro Leu Tyr Arg Glu Tyr Leu
195 200 205
Met Ser Leu Gly Ile Ala Ser Val Ser Arg Arg Ser Cys Glu Ala Leu
210 215 220
Leu Lys Arg Gly Gln Ser Ile Cys Ile Val Ile Gly Gly Ala Gln Glu
225 230 235 240
Ser Leu Leu Ala His Pro Gly His Met Asp Leu Val Leu Lys Arg Arg
245 250 255
Lys Gly Phe Ile Lys Leu Ala Leu Glu Val Gly Asn Thr Asp Leu Val
260 265 270
Pro Val Met Ala Phe Gly Glu Asn Asp Leu Tyr Gln Gln Val Asn Ser
275 280 285
Ser Lys Ser Ser Arg Leu Tyr Lys Leu Gln Ser Leu Val Lys Asn Ala
290 295 300
Leu Gly Phe Thr Leu Pro Leu Met His Ala Arg Gly Val Phe Asn Tyr
305 310 315 320
Asp Val Gly Ile Ile Pro Tyr Arg Arg Pro Ile Asn Val Val Val Gly
325 330 335
Lys Pro Ile Pro Ile Pro His Ile Pro Asn Pro Ser Ala Asp Gln Val
340 345 350
Asn Arg Tyr Gln Ile Gln Tyr Met Thr Glu Leu Lys Glu Leu Tyr Asp
355 360 365
Lys Tyr Lys Asp Lys Cys Ser Asn Lys Asp Leu Pro Val Pro Glu Leu
370 375 380
Thr Phe Val Glu
385
<210> 52
<211> 1167
<212> DNA
<213> 噬腺嘌呤阿克苏拉酵母
<400> 52
atggttcggt tcgctccttt aaatgttcct cttcatcgga ggttagagac gttcgcgctc 60
acctaccata tcctgtcgat tccagtatgg atgtccttct ttttgctatg ctgtgccatt 120
cctttaatgt ggccgttggt tatcatctac ctgctgtact atgcttccga caacagctct 180
gagaatggag gggttgcgag caggtattcg ccaaagttca ggtccgtgcc tctttggaag 240
tactttgcaa actactttcc aatcaccctt caccgtactc aagagctacc gcccgcattc 300
gtgtaccaag gcgaagactt ggaccctgag acgcccgatg acagtgacga cgggcatgca 360
aagtcaaagt ctattgtatt aaagctgtgg aaagttgcat tctggtggta ctacttgccc 420
aagcattttc ttcgcaaacc agaggttcgt cctacgggtc gaagatacat ctttggatat 480
cacccccatg gaatcattgg catgggtgcc attggcgcaa ttgctactga aggtgcgggg 540
tggtccaagc tcttccccgg gatccctgtc agtttgctca ctctggcaaa caactttcga 600
atccccctgt accgggaata tctcatgtct ctgggcattg cctcggtatc tagacggtcc 660
tgtgaagctt tattaaaaag aggacagtca atttgcattg taattggagg cgctcaggaa 720
agtcttcttg cacatccagg gcacatggat ttggtgctca agcgacgcaa gggattcatt 780
aaactagctc ttgaagttgg caacaccgac ttggtgccag ttatggcatt tggagaaaac 840
gatctctacc agcaagtgaa cagtagcaaa tcctcccgtc tatacaagct ccagagccta 900
gttaagaatg ccttgggatt cacgcttccg ctgatgcacg ctcgaggagt gttcaattat 960
gacgtgggca taatacccta tcgaagacca attaacgttg tagtgggcaa gcccatcccc 1020
attccacaca ttccaaaccc atctgccgac caggtcaatc ggtaccagat ccagtacatg 1080
actgaactca aagaattgta cgacaagtac aaagacaagt gcagtaacaa ggatcttcca 1140
gttccggagc ttacatttgt agagtag 1167
<210> 53
<211> 862
<212> PRT
<213> 酿酒酵母
<400> 53
Met Gln Tyr Val Gly Arg Ala Leu Gly Ser Val Ser Lys Thr Trp Ser
1 5 10 15
Ser Ile Asn Pro Ala Thr Leu Ser Gly Ala Ile Asp Val Ile Val Val
20 25 30
Glu His Pro Asp Gly Arg Leu Ser Cys Ser Pro Phe His Val Arg Phe
35 40 45
Gly Lys Phe Gln Ile Leu Lys Pro Ser Gln Lys Lys Val Gln Val Phe
50 55 60
Ile Asn Glu Lys Leu Ser Asn Met Pro Met Lys Leu Ser Asp Ser Gly
65 70 75 80
Glu Ala Tyr Phe Val Phe Glu Met Gly Asp Gln Val Thr Asp Val Pro
85 90 95
Asp Glu Leu Leu Val Ser Pro Val Met Ser Ala Thr Ser Ser Pro Pro
100 105 110
Gln Ser Pro Glu Thr Ser Ile Leu Glu Gly Gly Thr Glu Gly Glu Gly
115 120 125
Glu Gly Glu Asn Glu Asn Lys Lys Lys Glu Lys Lys Val Leu Glu Glu
130 135 140
Pro Asp Phe Leu Asp Ile Asn Asp Thr Gly Asp Ser Gly Ser Lys Asn
145 150 155 160
Ser Glu Thr Thr Gly Ser Leu Ser Pro Thr Glu Ser Ser Thr Thr Thr
165 170 175
Pro Pro Asp Ser Val Glu Glu Arg Lys Leu Val Glu Gln Arg Thr Lys
180 185 190
Asn Phe Gln Gln Lys Leu Asn Lys Lys Leu Thr Glu Ile His Ile Pro
195 200 205
Ser Lys Leu Asp Asn Asn Gly Asp Leu Leu Leu Asp Thr Glu Gly Tyr
210 215 220
Lys Pro Asn Lys Asn Met Met His Asp Thr Asp Ile Gln Leu Lys Gln
225 230 235 240
Leu Leu Lys Asp Glu Phe Gly Asn Asp Ser Asp Ile Ser Ser Phe Ile
245 250 255
Lys Glu Asp Lys Asn Gly Asn Ile Lys Ile Val Asn Pro Tyr Glu His
260 265 270
Leu Thr Asp Leu Ser Pro Pro Gly Thr Pro Pro Thr Met Ala Thr Ser
275 280 285
Gly Ser Val Leu Gly Leu Asp Ala Met Glu Ser Gly Ser Thr Leu Asn
290 295 300
Ser Leu Ser Ser Ser Pro Ser Gly Ser Asp Thr Glu Asp Glu Thr Ser
305 310 315 320
Phe Ser Lys Glu Gln Ser Ser Lys Ser Glu Lys Thr Ser Lys Lys Gly
325 330 335
Thr Ala Gly Ser Gly Glu Thr Glu Lys Arg Tyr Ile Arg Thr Ile Arg
340 345 350
Leu Thr Asn Asp Gln Leu Lys Cys Leu Asn Leu Thr Tyr Gly Glu Asn
355 360 365
Asp Leu Lys Phe Ser Val Asp His Gly Lys Ala Ile Val Thr Ser Lys
370 375 380
Leu Phe Val Trp Arg Trp Asp Val Pro Ile Val Ile Ser Asp Ile Asp
385 390 395 400
Gly Thr Ile Thr Lys Ser Asp Ala Leu Gly His Val Leu Ala Met Ile
405 410 415
Gly Lys Asp Trp Thr His Leu Gly Val Ala Lys Leu Phe Ser Glu Ile
420 425 430
Ser Arg Asn Gly Tyr Asn Ile Leu Tyr Leu Thr Ala Arg Ser Ala Gly
435 440 445
Gln Ala Asp Ser Thr Arg Ser Tyr Leu Arg Ser Ile Glu Gln Asn Gly
450 455 460
Ser Lys Leu Pro Asn Gly Pro Val Ile Leu Ser Pro Asp Arg Thr Met
465 470 475 480
Ala Ala Leu Arg Arg Glu Val Ile Leu Lys Lys Pro Glu Val Phe Lys
485 490 495
Ile Ala Cys Leu Asn Asp Ile Arg Ser Leu Tyr Phe Glu Asp Ser Asp
500 505 510
Asn Glu Val Asp Thr Glu Glu Lys Ser Thr Pro Phe Phe Ala Gly Phe
515 520 525
Gly Asn Arg Ile Thr Asp Ala Leu Ser Tyr Arg Thr Val Gly Ile Pro
530 535 540
Ser Ser Arg Ile Phe Thr Ile Asn Thr Glu Gly Glu Val His Met Glu
545 550 555 560
Leu Leu Glu Leu Ala Gly Tyr Arg Ser Ser Tyr Ile His Ile Asn Glu
565 570 575
Leu Val Asp His Phe Phe Pro Pro Val Ser Leu Asp Ser Val Asp Leu
580 585 590
Arg Thr Asn Thr Ser Met Val Pro Gly Ser Pro Pro Asn Arg Thr Leu
595 600 605
Asp Asn Phe Asp Ser Glu Ile Thr Ser Gly Arg Lys Thr Leu Phe Arg
610 615 620
Gly Asn Gln Glu Glu Lys Phe Thr Asp Val Asn Phe Trp Arg Asp Pro
625 630 635 640
Leu Val Asp Ile Asp Asn Leu Ser Asp Ile Ser Asn Asp Asp Ser Asp
645 650 655
Asn Ile Asp Glu Asp Thr Asp Val Ser Gln Gln Ser Asn Ile Ser Arg
660 665 670
Asn Arg Ala Asn Ser Val Lys Thr Ala Lys Val Thr Lys Ala Pro Gln
675 680 685
Arg Asn Val Ser Gly Ser Thr Asn Asn Asn Glu Val Leu Ala Ala Ser
690 695 700
Ser Asp Val Glu Asn Ala Ser Asp Leu Val Ser Ser His Ser Ser Ser
705 710 715 720
Gly Ser Thr Pro Asn Lys Ser Thr Met Ser Lys Gly Asp Ile Gly Lys
725 730 735
Gln Ile Tyr Leu Glu Leu Gly Ser Pro Leu Ala Ser Pro Lys Leu Arg
740 745 750
Tyr Leu Asp Asp Met Asp Asp Glu Asp Ser Asn Tyr Asn Arg Thr Lys
755 760 765
Ser Arg Arg Ala Ser Ser Ala Ala Ala Thr Ser Ile Asp Lys Glu Phe
770 775 780
Lys Lys Leu Ser Val Ser Lys Ala Gly Ala Pro Thr Arg Ile Val Ser
785 790 795 800
Lys Ile Asn Val Ser Asn Asp Val His Ser Leu Gly Asn Ser Asp Thr
805 810 815
Glu Ser Arg Arg Glu Gln Ser Val Asn Glu Thr Gly Arg Asn Gln Leu
820 825 830
Pro His Asn Ser Met Asp Asp Lys Asp Leu Asp Ser Arg Val Ser Asp
835 840 845
Glu Phe Asp Asp Asp Glu Phe Asp Glu Asp Glu Phe Glu Asp
850 855 860
<210> 54
<211> 2589
<212> DNA
<213> 酿酒酵母
<400> 54
atgcagtacg taggcagagc tcttgggtct gtgtctaaaa catggtcttc tatcaatccg 60
gctacgctat caggtgctat agatgtcatt gtagtggagc atccagacgg aaggctatca 120
tgttctccct ttcatgtgag gttcggcaaa tttcaaattc taaagccatc tcaaaagaaa 180
gtccaagtgt ttataaatga gaaactgagt aatatgccaa tgaaactgag tgattctgga 240
gaagcctatt tcgttttcga gatgggtgac caggtcactg atgtccctga cgaattgctt 300
gtgtcgcccg tgatgagcgc cacatcaagc ccccctcaat cacctgaaac atccatctta 360
gaaggaggaa ccgagggtga aggtgaaggt gaaaatgaaa ataagaagaa ggaaaagaaa 420
gtgctagagg aaccagattt tttagatatc aatgacactg gagattcagg cagtaaaaat 480
agtgaaacta cagggtcgct ttctcctact gaatcctcta caacgacacc accagattca 540
gttgaagaga ggaagcttgt tgagcagcgt acaaagaact ttcagcaaaa actaaacaaa 600
aaactcactg aaatccatat acccagtaaa cttgataaca atggcgactt actactagac 660
actgaaggtt acaagccaaa caagaatatg atgcatgaca cagacataca actgaagcag 720
ttgttaaagg acgaattcgg taatgattca gatatttcca gttttatcaa ggaggacaaa 780
aatggcaaca tcaagatcgt aaatccttac gagcacctta ctgatttatc tcctccaggt 840
acgcctccaa caatggccac aagcggatca gttttaggct tagatgcaat ggaatcagga 900
agtactttga attcgttatc ttcttcacct tctggttccg atactgagga cgaaacatca 960
tttagcaaag aacaaagcag taaaagtgaa aaaactagca agaaaggaac agcagggagc 1020
ggtgagaccg agaaaagata catacgaacg ataagattga ctaatgacca gttaaagtgc 1080
ctaaatttaa cttatggtga aaatgatctg aaattttccg tagatcacgg aaaagctatt 1140
gttacgtcaa aattattcgt ttggaggtgg gatgttccaa ttgttatcag tgatattgat 1200
ggcaccatca caaaatcgga cgctttaggc catgttctgg caatgatagg aaaagactgg 1260
acgcacttgg gtgtagccaa gttatttagc gagatctcca ggaatggcta taatatactc 1320
tatctaactg caagaagtgc tggacaagct gattccacga ggagttattt gcgatcaatt 1380
gaacagaatg gcagcaaact accaaatggg cctgtgattt tatcacccga tagaacgatg 1440
gctgcgttaa ggcgggaagt aatactaaaa aaacctgaag tctttaaaat cgcgtgtcta 1500
aacgacataa gatccttgta ttttgaagac agtgataacg aagtggatac agaggaaaaa 1560
tcaacaccat tttttgccgg ctttggtaat aggattactg atgctttatc ttacagaact 1620
gtggggatac ctagttcaag aattttcaca ataaatacag agggtgaggt tcatatggaa 1680
ttattggagt tagcaggtta cagaagctcc tatattcata tcaatgagct tgtcgatcat 1740
ttctttccac cagtcagcct tgatagtgtc gatctaagaa ctaatacttc catggttcct 1800
ggctcccccc ctaatagaac gttggataac tttgactcag aaattacttc aggtcgcaaa 1860
acgctattta gaggcaatca ggaagagaaa ttcacagacg taaatttttg gagagacccg 1920
ttagtcgaca tcgacaactt atcggatatt agcaatgatg attctgataa catcgatgaa 1980
gatactgacg tatcacaaca aagcaacatt agtagaaata gggcaaattc agtcaaaacc 2040
gccaaggtca ctaaagcccc gcaaagaaat gtgagcggca gcacaaataa caacgaagtt 2100
ttagccgctt cgtctgatgt agaaaatgcg tctgacctgg tgagttccca tagtagctca 2160
ggatccacgc ccaataaatc tacaatgtcc aaaggggaca ttggaaaaca aatatatttg 2220
gagctaggtt ctccacttgc atcgccaaaa ctaagatatt tagacgatat ggatgatgaa 2280
gactccaatt acaatagaac taaatcaagg agagcatctt ctgcagccgc gactagtatc 2340
gataaagagt tcaaaaagct ctctgtgtca aaggccggcg ctccaacaag aattgtttca 2400
aagatcaacg tttcaaatga cgtacattca cttgggaatt cagataccga atcacgaagg 2460
gagcaaagtg ttaatgaaac agggcgcaat cagctacccc acaactcaat ggacgataaa 2520
gatttggatt caagagtaag cgatgaattc gatgacgatg aattcgacga agatgaattc 2580
gaagattaa 2589
<210> 55
<211> 742
<212> PRT
<213> 噬腺嘌呤阿克苏拉酵母
<400> 55
Met Gln Tyr Val Gly Arg Ala Ile Gly Ser Val Ser Lys Thr Trp Asn
1 5 10 15
Ser Ile Asn Pro Ala Thr Leu Ser Gly Ala Ile Asp Ile Met Val Val
20 25 30
Glu Gln Pro Asp Gly Thr Leu Ser Cys Ser Pro Phe His Val Arg Phe
35 40 45
Gly Lys Phe Ser Leu Leu Arg Pro Ser Gln Lys Lys Val Gln Phe Ser
50 55 60
Val Asn Gly Glu Ala Thr Asp Leu Pro Met Lys Leu Gly Asp Gly Gly
65 70 75 80
Glu Ala Phe Phe Val Phe Glu Thr Pro Asn Lys Val Pro Ser Asp Leu
85 90 95
Leu Thr Ser Pro Val Val Ser Pro Ser Ser Ser Pro Glu Ser Ile Val
100 105 110
Ala Asp Asp Glu Asp Ala Glu Gly Arg Pro Leu Gln Glu Pro Glu Phe
115 120 125
Leu Asp Leu Ser Arg Thr Glu Pro Glu Arg Ala Arg Ser Glu Ser Pro
130 135 140
Gln Arg Pro Pro Asn Lys Asp Gly Ser Leu Arg Ser Ala Asp Ser Phe
145 150 155 160
Pro Pro Pro Pro Ala Asn Asn Ala Gln Trp Ser Glu Ser Ser Pro Ser
165 170 175
Leu Thr Glu Ala Pro Pro Leu Ser Pro Gly Glu Pro Glu Pro Glu Arg
180 185 190
Ala Ile Ser Lys Asp Arg Val His Ser Leu Thr Lys Arg Leu Thr Asp
195 200 205
Ile Asn Ile Pro Ser Lys Ile Thr Asp Asn Gly Asp Ile Val Leu Asp
210 215 220
Met Thr Gly Tyr Lys Ser Gly Ala Asp Glu Phe Arg Thr Ser Glu Ala
225 230 235 240
Val Val Lys Lys Leu Leu Ala Glu Glu Leu Gly Leu Gly Pro Glu Leu
245 250 255
Asp Phe Glu Ser Ile Met Gly Pro Asp Glu Glu Gly Asn Ile Arg Ile
260 265 270
Tyr Ser Arg Asp Asp Leu Tyr Ala Ala Thr Ala Gly His Arg His Ser
275 280 285
His Ser His His Pro Phe Pro Ser Ala Glu Asp Thr Ala Pro Val Thr
290 295 300
Pro Ala Pro Glu Ser Ser Ser Ser Ser Ser Glu Pro Ala Ser Thr Glu
305 310 315 320
Gly Thr Pro Thr Ala Gly Glu Pro Phe Tyr Ala Lys Thr Leu Arg Leu
325 330 335
Thr Ser Asp Gln Leu Lys Lys Leu Asn Leu Lys Ser Gly Arg Asn Glu
340 345 350
Val Gln Phe Lys Val Leu Gln Asn Lys Ala Val Ile Asn Ala His Leu
355 360 365
Tyr Tyr Trp Lys Ser Asn Ala Pro Ile Val Ile Ser Asp Ile Asp Gly
370 375 380
Thr Ile Thr Lys Ser Asp Ala Leu Gly His Val Leu Thr Met Leu Gly
385 390 395 400
Arg Asp Trp Thr His Ser Gly Val Ala Lys Leu Tyr Val Asp Ile Ala
405 410 415
Asn Asn Gly Tyr Asn Ile Val Tyr Leu Thr Ala Arg Ser Val Gly Gln
420 425 430
Ala Asp Ala Thr Arg Tyr Tyr Leu Gln Gly Ile Glu Gln Glu Gly Tyr
435 440 445
Arg Met Pro Pro Gly Pro Val Ile Leu Ser Pro Asp Arg Thr Met Ala
450 455 460
Ala Leu Arg Arg Glu Val Ile Met Arg Lys Pro Glu Val Phe Lys Met
465 470 475 480
Ala Ala Leu Arg Asp Ile Gln Ser Leu Tyr Asp Tyr Lys Glu Gly Thr
485 490 495
Pro Phe Tyr Ala Gly Phe Gly Asn Arg Ile Thr Asp Ala Leu Ser Tyr
500 505 510
Arg Ser Val Gly Ile Pro Ser Ser Lys Ile Phe Thr Ile Asn Thr Asn
515 520 525
Ser Glu Val His Met Glu Leu Leu Glu Leu Ala Gly Tyr Arg Ser Ser
530 535 540
Tyr Val His Ile Ala Asp Leu Val Asp His Phe Phe Pro Pro Val Val
545 550 555 560
Ala Ser Gly Gly Lys Asp Val Glu Asn Lys Tyr Ser Asp Val Asn Phe
565 570 575
Trp Arg Asp Pro Ile Pro Asp Ile Ser Asp Leu Glu Ser Leu Asp Gly
580 585 590
Phe Glu Asp Glu Gly Pro Lys Ser Pro Lys Ser Pro Glu Gln Arg Ala
595 600 605
Lys Ser Pro Ser Asp Met Leu Ser Ser Ser Gln Ser Pro Gln Lys Ala
610 615 620
Asn Glu Lys Thr Ser Asp Lys Ser Gly Pro Met Asp Met Gly Pro Pro
625 630 635 640
Val Met Ser Ser Glu Lys Lys Ser Asp Lys Gly Glu Glu Asp Glu Asp
645 650 655
Asp Glu Asp Tyr Asp Glu Asp Tyr Glu Asp Asp Asn Tyr Glu Asp Glu
660 665 670
Tyr Asp Asp Tyr Glu Tyr Asp Glu Asp Glu Tyr Asp Asp Tyr Asp Tyr
675 680 685
Glu Asn Glu Asp Tyr Gln Asp Gly Glu Thr Pro Asp Glu Arg Gln Gly
690 695 700
Arg Ser Arg Gln Arg Arg Ser Ile Val Tyr Thr His Phe Gly Ala Arg
705 710 715 720
Glu Pro Ala His Leu Glu Gln Ala Arg Thr Leu Arg Glu Thr Leu Lys
725 730 735
Asp Ile Asn Thr Asn Leu
740
<210> 56
<211> 2229
<212> DNA
<213> 噬腺嘌呤阿克苏拉酵母
<400> 56
atgcagtacg taggacgagc gattggatct gtgtccaaga catggaactc catcaaccct 60
gctacgctgt cgggagcaat tgatatcatg gttgtggaac agcccgatgg gactctgtcg 120
tgctctccct ttcatgtccg gttcggcaag ttctcgttgc ttcgtccctc gcagaagaag 180
gtccagtttt ccgtaaatgg ggaggccact gacttgccaa tgaagttggg ggatggcggc 240
gaagcgttct ttgtgtttga gactcccaac aaggtcccca gtgacctgtt aacttcaccg 300
gtagtgtccc cgtcgtcttc accagaatcg atcgtagcgg atgatgagga tgccgaagga 360
cgccctttgc aagagccgga gtttttggat ctttcccgta ctgaacctga gagagctcgc 420
tctgaaagcc ctcagcggcc acccaacaag gacggatctc tacgaagcgc tgattcgttc 480
cctccgccac ctgccaacaa cgcccaatgg agcgagtcca gtcctagttt gaccgaggcg 540
ccgccgctgt ctccaggaga gcctgaacca gagcgggcca tttcaaagga ccgggttcat 600
agtctgacca agagattgac tgatatcaat atcccatcca agatcaccga caatggcgac 660
attgtgctgg acatgaccgg gtacaagagc ggtgcggatg agtttagaac ttcagaagca 720
gtagtaaaga agcttttggc tgaggagctt gggctgggtc ccgagctcga ttttgaatcc 780
atcatgggcc ccgacgagga gggcaatatt cgtatttatt cccgtgacga tttgtatgct 840
gccacggcag gccaccgcca tagtcactct catcatccct tccctagtgc tgaggacact 900
gcgccggtga cccctgcccc tgagtcctct tcgtcatcat ctgaacctgc atctaccgaa 960
ggcactccca ccgccggaga gcccttctat gcaaagacgt tgcgattgac ttcggatcag 1020
ctcaagaagc tcaatctcaa gtctgggcgg aatgaggtcc agtttaaggt gctccaaaac 1080
aaggccgtga tcaacgctca cctgtactac tggaaaagca acgccccaat cgtcatttct 1140
gatatcgatg gaaccattac aaagtcggat gccctgggcc atgtcttgac catgcttggg 1200
cgggactgga ctcactctgg ggtcgccaaa ttatatgtgg atattgctaa caatgggtac 1260
aatattgtat atttgaccgc acgatcagtt ggccaggcag atgctactcg atattatctt 1320
cagggaattg agcaagaagg gtatagaatg ccccctggtc cagttatttt gtctccagat 1380
aggacaatgg ctgccttgcg ccgagaggtt atcatgcgca aacccgaggt gttcaagatg 1440
gccgcccttc gggatatcca gtctctgtac gactataaag agggcacccc attttatgca 1500
gggtttggta atcgtatcac cgacgcgcta tcttatcgat cggtgggaat cccttcgtcg 1560
aagatcttca ccatcaatac caattctgaa gtccacatgg aactcctgga gttggcaggg 1620
taccgctcca gctacgtcca tattgccgac ctcgtggacc actttttccc tccagtggtg 1680
gccagtgggg gtaaggatgt ggaaaacaag tattccgatg tcaatttctg gcgagaccct 1740
atccctgata tttccgacct ggaatctcta gatggatttg aagatgaagg accaaagagt 1800
ccaaagagcc ctgagcagcg agccaaatca ccatctgata tgctttctag tagtcaatcc 1860
cctcagaaag ctaacgagaa gacctccgac aaatcagggc ccatggacat gggtcctcca 1920
gtcatgtcat ccgaaaagaa gagcgataag ggcgaagagg acgaggatga tgaggactac 1980
gatgaggact acgaagacga caattacgag gatgagtatg atgactacga gtatgacgag 2040
gatgaatatg atgactatga ttacgagaac gaggactatc aagacggaga gacccctgat 2100
gaaagacagg gtcgctctag gcaacggcga tcgattgtat acacccattt cggtgcccga 2160
gagcccgccc atttggagca ggcacgcact ctgagagaga ctctcaagga tatcaatact 2220
aacttgtaa 2229
<210> 57
<211> 723
<212> PRT
<213> 解脂耶氏酵母
<400> 57
Met Lys Val Gly Asp Gly Gly Glu Val Phe Phe Val Phe Glu Thr Asp
1 5 10 15
Ala Asp Val Pro Glu Glu Leu Leu Thr Ser Pro Val Ile Ser Pro Ser
20 25 30
Ser Ser Pro Ser Trp Gly Gln Glu Glu Gly Gly Asp Gly Glu Pro Asp
35 40 45
Tyr Leu Ala Leu Asn Asp Ser Lys Gln Gly Gly Asp Ser Lys His Gly
50 55 60
Arg Ser Pro Ser Glu Gly Pro Pro Phe Arg Ser Pro Ser Ala Asp His
65 70 75 80
Leu His Glu Met Gly Ser Phe Asp Asp Glu Asn Asp Pro Glu Val Asn
85 90 95
Arg Arg Gln Arg Ala Ser Thr Ala Ala Pro Glu Pro Val Pro Gly Ser
100 105 110
Leu Lys His Pro Ala Thr Ile Ser Glu Gly Ile Ser Ser Ala Ser Phe
115 120 125
Ser Asn Ser Asp Thr Asp Arg Thr Asp Thr Ser Gly Pro Thr Glu Thr
130 135 140
Glu Pro Thr Glu Leu Thr Glu Pro Thr Glu Pro Thr Glu Pro Thr Glu
145 150 155 160
Pro Leu Asp Leu Glu Gln Ser Leu His Arg Ala Ala Thr Ser Pro Ala
165 170 175
Pro Ser Ser Glu Glu Ile Trp Glu Lys Ala Arg Ala Leu Ser Lys Lys
180 185 190
Leu Thr Ser Glu Asn Ile Gln Ser Lys Ile Ser Asp Asn Gly Asp Ile
195 200 205
Ile Leu Asp Met Thr Gly Tyr Lys Tyr Asp His Glu Asp Val Ser Arg
210 215 220
Ser Glu Glu Leu Val Lys Lys Ile Leu Ala Glu Glu Leu Gly Glu Asp
225 230 235 240
Arg Asp Leu Ser His Ile Leu Val Glu Asp Glu Glu Gly Asn Leu Val
245 250 255
Ile Gln Ser Ala Gly Asp Ser His His His Glu His Met Ser Ser Pro
260 265 270
Glu Ser Leu Ala His Ser Pro Gln Pro Leu Pro Ser Ser Asn Leu Pro
275 280 285
Ser Gln Ala Ser Asp Asn Lys His Tyr Ala Lys Thr Ile Arg Leu Thr
290 295 300
Ser Asp Gln Leu Lys Ser Leu Asp Leu Lys Pro Gly Lys Asn Glu Val
305 310 315 320
Thr Phe Ala Val Asn Asn Gly Lys Thr Ser Cys Ser Ala Gln Leu Phe
325 330 335
Tyr Trp Lys Tyr Asp Ile Pro Val Val Ile Ser Asp Ile Asp Gly Thr
340 345 350
Ile Thr Lys Ser Asp Ala Leu Gly His Leu Leu Thr Met Met Gly Arg
355 360 365
Asp Trp Thr His Thr Gly Val Ala Lys Leu Phe Ser Asp Ile Arg Ala
370 375 380
Asn Gly Tyr Asn Ile Met Tyr Leu Thr Ala Arg Ser Val Gly Gln Ala
385 390 395 400
Asp Ala Thr Arg Ala Tyr Leu Gly Gly Val Asp Gln Phe Gly Phe Lys
405 410 415
Leu Pro Pro Gly Pro Val Ile Leu Ser Pro Asp Arg Thr Leu Ala Ala
420 425 430
Leu Lys Arg Glu Val Ile Leu Lys Lys Pro Glu Val Phe Lys Met Ala
435 440 445
Cys Leu Arg Asp Ile Lys Ser Leu Phe Gly Glu Thr Glu Asp Ala Thr
450 455 460
Asn Pro Phe Tyr Ala Gly Phe Gly Asn Arg Ile Thr Asp Ala Leu Ser
465 470 475 480
Tyr Arg Ser Val Gly Val Pro Ser Ser Arg Ile Phe Thr Ile Asn Ser
485 490 495
Asn Ala Glu Val His Met Glu Leu Leu Glu Leu Ala Gly Tyr Lys Ser
500 505 510
Ser Tyr Val His Ile Ala Asp Leu Val Asp His Phe Phe Pro Pro Glu
515 520 525
Ser Glu Phe Thr Thr Ile Gln Glu Glu Lys Tyr Thr Asp Val Asn Tyr
530 535 540
Trp Arg Asp Pro Ile Ile Asp Leu Ser Asp Leu Thr Asp Asp Glu Leu
545 550 555 560
Thr Asp Asp Asp Glu Leu Ser Lys Ser Pro Lys Ser Pro Arg Ser Pro
565 570 575
Arg Ser Pro Arg Ala Gly Ser Ala Gly Ser Ser Ala Ala Pro Ser Gly
580 585 590
Ser Gly Ala Asp Pro Ala Gly Pro Ser Glu Pro Lys Asp Ser Ala Asn
595 600 605
Pro Ser Lys Phe Ser Tyr Lys Lys Ala Pro Thr Asn Ser Arg Phe Gln
610 615 620
Pro Val Ser Tyr Asp Leu Asp Leu Asp Asp Gly Tyr Glu Tyr Asp Asp
625 630 635 640
Asp Asp Asp Tyr Asp Asp Asp Glu Glu Phe Val Asp Ala Glu Ser Asp
645 650 655
Ala Leu Glu Glu Asp Asp Asp Asp Asp Asp Asp Val Asp Leu Asp Asn
660 665 670
Asp Ser Asp His Ser Pro Val Lys Pro Pro Ser Gln Met Gln Arg Val
675 680 685
Ile Asn Lys Thr Ile Glu Asp Asn Lys Gly Leu His Met Asp Glu Asp
690 695 700
Asp Val Gln Lys Ala Met Lys Ala Leu Lys Met Glu Arg Ala Ser Ile
705 710 715 720
Asn Pro Glu
<210> 58
<211> 2172
<212> DNA
<213> 解脂耶氏酵母
<400> 58
atgaaggtgg gcgatggagg agaagtcttc tttgtgttcg agaccgacgc agacgtgccc 60
gaagagctcc tgacatcccc cgtcatttct ccctcttcgt cgccatcctg gggccaggag 120
gaaggcgggg atggtgagcc ggactacctt gctctgaacg actctaaaca gggtggcgac 180
agcaagcacg gcagatcgcc ctcggagggc ccaccattca gatcaccttc ggcggatcac 240
ttacatgaga tgggcagctt cgatgatgag aatgaccctg aggtgaacag aagacaacgt 300
gcgagcacgg cagctccaga gcctgttcct ggttcgttga aacacccagc cactatctcg 360
gaaggcatct cttcggcctc gttttccaac agcgatactg atcgaacaga cacttctgga 420
cccacagaga cagaacccac agagctcaca gagcctacag agcccacaga gcccacagag 480
cctctggatc ttgagcagag tctccaccgg gctgccactt ctcccgcccc ttcgtccgag 540
gagatttggg agaaggcccg tgcactgtcc aagaaactca catcagaaaa cattcagagt 600
aaaatctccg acaacggaga cattattctg gatatgactg gttacaagta cgaccacgag 660
gacgtgagtc gatcagagga gctggtcaag aaaatcctcg ctgaggaact gggagaagac 720
agagacctgt cccacatcct ggttgaagac gaggagggta accttgtgat tcagagcgct 780
ggagacagcc accatcacga gcatatgagc tcgcccgagt ctctggccca ctcccctcag 840
cccctccctt cttctaacct tccgtctcag gcctcggaca acaagcacta cgccaagacc 900
atccgtttga cgtctgacca gctcaagtct ctggatctca agcccggcaa gaacgaggtc 960
acctttgctg tcaataacgg caagacgtcg tgttcggccc agctgttcta ctggaagtac 1020
gacattcctg ttgtcatttc cgacattgat ggcacgatca ccaagtccga tgctctgggc 1080
catctgctca ccatgatggg ccgagactgg acccacaccg gcgtggccaa gctcttttcc 1140
gatatcagag ccaacgggta taatatcatg tatctgacag cacgatcagt gggacaggca 1200
gatgcaacca gggcatatct aggcggtgtt gaccagtttg gcttcaagct gcctccagga 1260
cccgtcatct tgtcgcctga tagaaccctg gcggctctca agagagaggt gattcttaag 1320
aaacctgagg tattcaagat ggcgtgtctg cgggacatta agtcgctgtt tggcgagacc 1380
gaagacgcca ccaatccatt ctacgctgga tttggcaacc gaatcaccga cgcgttgtcg 1440
tatagatctg tcggtgtgcc gtcgtctaga atcttcacaa tcaactcgaa cgccgaggtc 1500
catatggagc tgcttgaact ggctggctac aagtcctcgt atgtccacat tgccgatctt 1560
gtcgaccact ttttccctcc ggaaagcgag ttcacgacca ttcaggagga aaaatacacg 1620
gacgtcaact actggcgaga tcccattatt gacctgtctg atctgaccga cgacgagctg 1680
actgacgatg atgagctctc caagtcgccc aagtcgccca gatctcctag aagcccgcgg 1740
gccggttcgg caggctccag cgcggctccc tcaggctcgg gcgccgaccc tgccggaccc 1800
tccgagccga aggactccgc gaacccgtcg aagttcagct ataagaaggc tcctacgaac 1860
tctcgattcc agcccgtttc gtacgatctt gatcttgacg acggatacga gtacgacgat 1920
gacgatgact atgatgacga tgaggagttt gtggacgctg agagcgacgc gctggaggag 1980
gatgacgacg atgatgatga cgtcgaccta gacaacgact ctgaccactc ccctgtcaag 2040
ccgccctcgc agatgcagcg agtcatcaac aagactattg aggacaacaa gggcctgcac 2100
atggatgagg atgacgttca aaaagccatg aaggccctga agatggaacg agcaagcatc 2160
aatcctgagt aa 2172
<210> 59
<211> 282
<212> PRT
<213> 解脂耶氏酵母
<400> 59
Met Ser Val Ala Ser Lys Leu Val Phe Tyr Val Arg Ala Ala Ile Ala
1 5 10 15
Val Val Ile Phe Ala Ala Cys Ala Thr Tyr Gly Val Leu Ala Ser Thr
20 25 30
Ile Leu Thr Ala Ile Gly Lys Gln Gly Leu Ala Gln Trp Thr Val Ala
35 40 45
Arg Ala Phe Tyr Tyr Ser Val Arg Ile Phe Leu Gly Ile Ser Ile Lys
50 55 60
Leu Arg Ser Arg Gln Val Thr Gly Thr Ala Gly Leu Asp Ala Ser Lys
65 70 75 80
Ile Gln Val Ala Asn Thr Thr Lys Pro Ile Asp Asp Ile Thr Lys His
85 90 95
Leu Pro Arg Pro Cys Ile Leu Ile Ser Asn His Gln Asn Glu Met Asp
100 105 110
Ile Leu Val Leu Gly Arg Ile Phe Pro Gln Tyr Cys Ser Val Thr Ala
115 120 125
Lys Lys Ala Leu Lys Trp Tyr Pro Leu Leu Gly Gln Phe Met Ala Leu
130 135 140
Ser Gly Thr Ile Phe Leu Asp Arg Lys Asp Arg Thr Lys Ser Val Gln
145 150 155 160
Thr Leu Gly Gly Ala Val Lys Thr Ile Gln Ser Gly Asn Gly Gly Lys
165 170 175
Gly Gln Ser Val Phe Met Phe Pro Glu Gly Thr Arg Ser Tyr Ser Lys
180 185 190
Asp Val Gly Ile Met Pro Phe Lys Lys Gly Cys Phe His Leu Ala Val
195 200 205
Gln Ser Gly Ala Pro Ile Val Pro Val Val Val Gln Asn Thr Ser Arg
210 215 220
Met Phe Ser Phe Gly Arg Gly Lys Leu Asp Ala Gly Glu Ile Leu Val
225 230 235 240
Asp Val Leu Ser Pro Ile Glu Thr Lys Gly Leu Asp Ala Ser Asn Val
245 250 255
Asp Ala Leu Met Ala Thr Thr Tyr Lys Ala Met Cys Glu Thr Ala Asp
260 265 270
Gln Ile Gly Tyr Ala Gly Gln Lys Thr Gln
275 280
<210> 60
<211> 849
<212> DNA
<213> 解脂耶氏酵母
<400> 60
atgtccgttg catccaagct cgtcttctac gtccgcgccg ccatcgccgt ggtcatcttt 60
gccgcctgtg ccacctacgg cgtgctggcg tccaccattc tcaccgccat cggcaagcag 120
ggcctggccc aatggaccgt tgccagagcc ttctactact cggtgcgcat cttcctgggt 180
atcagcatca agctgcgtag ccggcaggtg accggaaccg ccggtctgga tgcctccaag 240
atccaggtcg ccaacaccac caagcccatt gacgacatca ccaaacacct gccccgacca 300
tgcattctga tttccaacca ccagaacgaa atggacattc tggtgctcgg tcgcatcttc 360
ccccagtact gctccgtcac cgccaaaaag gccctcaagt ggtaccctct gctgggccag 420
ttcatggcgc tgtccggcac catcttcctg gaccgaaagg accgaaccaa gtccgtgcag 480
accctcggcg gcgccgtcaa gaccatccag agcggcaacg gaggcaaggg ccagagcgtc 540
ttcatgttcc ccgagggaac ccgatcctac tccaaggacg tcggcatcat gcccttcaag 600
aagggctgtt tccacctggc ggtccagtcg ggcgctccca ttgtccccgt ggtggtccag 660
aacacctccc gaatgttttc tttcggccga ggcaagctgg acgccggaga gatccttgtc 720
gacgtcctga gccccattga gaccaagggt ctggacgcca gcaacgtcga cgctctcatg 780
gccaccactt ataaggccat gtgcgagact gccgaccaga ttggctacgc tggccagaag 840
actcagtag 849
<210> 61
<211> 281
<212> PRT
<213> 噬腺嘌呤阿克苏拉酵母
<400> 61
Met Ala Thr Val Val Lys Gln Leu Val Gln Tyr Leu Pro Lys Ala Val
1 5 10 15
Ala Gly Thr Val Val Leu Gly Tyr Phe Gly Ile Ile Lys Pro Leu Gln
20 25 30
Phe Tyr Val Arg Leu Ala Val Tyr Ile Leu Ala Val Ala Phe Cys Ala
35 40 45
Phe Asn Gly Ala Val Leu Ser Ala Val Leu Thr Leu Ile Gly Lys Gln
50 55 60
Gly Leu Ser Gln Trp Cys Val Ala Arg Met Phe Leu Tyr Ile Ala Gly
65 70 75 80
Thr Ile Leu Gly Ile Lys Val Val Ile Lys Asn Pro Glu Arg Leu Lys
85 90 95
Thr Arg Pro Ala Val Phe Ile Ser Asn His Gln Ser Glu Leu Asp Ile
100 105 110
Leu Ile Leu Gly Ala Thr Phe Pro Gln Tyr Cys Ser Val Thr Ala Lys
115 120 125
Lys Ser Leu Lys Tyr Tyr Pro Phe Leu Gly Trp Phe Met Ala Leu Ser
130 135 140
Gly Ser Val Phe Ile Asp Arg Ala Asn Arg Asp Asn Ala Leu Lys Ala
145 150 155 160
Phe Glu Gly Ala Ala Lys Lys Val Asn Arg Asp Lys Gln Ser Val Phe
165 170 175
Met Phe Pro Glu Gly Thr Arg Ser Tyr Tyr Gln Glu Pro Gly Leu Leu
180 185 190
Pro Phe Lys Lys Gly Ala Phe His Phe Ala Val Gln Ala Gly Val Pro
195 200 205
Ile Val Pro Phe Val Val Ser Asn Tyr Ser Lys Val Val Asn Phe Lys
210 215 220
Lys Arg Ile Phe Glu Pro Gly Val Ile Glu Ile Glu Val Leu Glu Pro
225 230 235 240
Ile Lys Val Thr Gly Lys Glu Thr Lys Glu Glu Ile Asn Gln Leu Val
245 250 255
Glu Gly Thr Arg Glu Arg Met Leu Glu Ser Thr Gln Arg Leu Gly Tyr
260 265 270
Gly Ser Glu Tyr Asn Lys Lys Ser Asn
275 280
<210> 62
<211> 846
<212> DNA
<213> 噬腺嘌呤阿克苏拉酵母
<400> 62
atggcgacgg ttgtaaagca gctggttcag tatctgccaa aggccgtggc aggaacggtt 60
gttctaggct attttggcat tatcaagccc ctacagtttt acgtgcggtt ggcggtttac 120
attctggcgg ttgccttttg cgcgttcaat ggagcggtgc tgtctgcggt gctgactttg 180
attggaaagc agggtctttc gcaatggtgt gtggccagaa tgttcctgta cattgcggga 240
acgattctgg gaatcaaggt tgtgattaag aaccccgagc gtctcaagac ccgacctgcg 300
gtgttcatct ccaaccacca gtccgagctg gacattctca tcttgggagc tactttccct 360
cagtactgta gtgtgacggc aaagaagagt ctcaagtact acccgttctt gggatggttc 420
atggctctga gtggatcggt gtttattgac cgagccaatc gagacaatgc tctcaaggct 480
tttgagggag cggccaagaa ggtgaaccgt gacaagcaga gcgtgttcat gttccccgag 540
ggcactcgat cgtactacca ggagcccggt ctattgccat ttaagaaggg agcgttccac 600
tttgccgtcc aggctggtgt tcccattgtg ccatttgtag tgagcaacta ctccaaggtg 660
gtgaacttta agaagcgaat tttcgagcct ggtgtgattg aaattgaggt gttggagccc 720
atcaaggtca ctggaaagga gaccaaggag gagattaacc aattggtcga aggtactagg 780
gagcgcatgc tggagagcac ccagcgattg ggctatggct ctgagtacaa caagaagagc 840
aactaa 846
<210> 63
<211> 579
<212> PRT
<213> 噬腺嘌呤阿克苏拉酵母
<400> 63
Met Ala Leu Gly Val Phe Ile Gln Leu Ala Glu Glu Gly Ser Arg Met
1 5 10 15
Thr Gly Ile Pro Pro Asp Leu Leu Arg Leu Ala Phe Cys Leu Val Phe
20 25 30
Ser Tyr Pro Gly Cys Ala Ile Leu Lys Arg Leu Pro Asp Asn Asn Thr
35 40 45
Leu Val Lys Glu Leu Phe Ile Met Ser Val Ser Leu Phe Tyr Leu Leu
50 55 60
Gly Val Phe Ser Met Trp Gly Gly Val Arg Thr Leu Leu Ile Ser Thr
65 70 75 80
Leu Ala Thr Tyr Tyr Ile Thr Lys Lys Trp Pro Ser Ser Pro Phe Met
85 90 95
Pro Trp Ala Asn Phe Leu Phe Val Met Ala His Leu Phe Thr Asn His
100 105 110
Ile Ala Asn Gln Ile Lys Glu Ala Gly Glu Leu Tyr Asp Pro Asn Val
115 120 125
Ile Asp Ile Thr Gly Ala Gln Met Val Leu Cys Met Lys Leu Ser Ala
130 135 140
Phe Gly Trp Asn Val Tyr Asp Gly Thr Gln Pro Gln Gly Ser Leu Ser
145 150 155 160
Asp Phe Gln Lys Met Arg Ala Val Lys Lys His Pro Ser Leu Leu Asp
165 170 175
Phe Val Thr Tyr Ala Phe Phe Phe Pro Ser Val Leu Thr Gly Pro Ser
180 185 190
Phe Asp Tyr Glu Glu Phe Arg Gln Trp Ile Asp Leu Ser Met Phe Asp
195 200 205
Val Thr Ala Asn Asp Pro Lys Arg Gly Arg Ala Val Asn Arg Lys Ile
210 215 220
Pro Arg Ser Gly Arg Val Ala Thr Leu Lys Ala Leu Glu Gly Val Leu
225 230 235 240
Trp Ile Val Val Trp Val Leu Val Thr Ser Tyr Phe Asn Leu Asp Tyr
245 250 255
Ala Leu Ser Pro Lys Phe Thr Ser Glu Leu Asn Phe Val Leu Lys Met
260 265 270
Leu Tyr Leu Tyr Val Leu Gly Phe Ser Tyr Arg Leu Lys Tyr Tyr Gly
275 280 285
Ala Trp Ser Ile Ser Glu Gly Ser Cys Ile Leu Ala Gly Ile Gly Phe
290 295 300
Asn Gly Lys Thr Lys Ser Gly Lys Tyr Lys Trp Asp Arg Val Lys Asn
305 310 315 320
Ile Asp Pro Trp Lys Phe Glu Phe Gly Gln Asn Thr Phe Thr Leu Leu
325 330 335
Glu Ala Trp Asn Met Asn Thr Asn Lys Trp Leu Lys Asn Tyr Val Tyr
340 345 350
Leu Arg Val Thr Pro Lys Gly Lys Lys Pro Gly Phe Arg Ser Thr Leu
355 360 365
Ala Thr Phe Phe Thr Ser Ala Phe Trp His Gly Thr Arg Pro Gly Tyr
370 375 380
Tyr Leu Thr Phe Val Thr Gly Ala Phe Phe Gln Ala Leu Gly Lys Ile
385 390 395 400
Phe Arg Arg Asn Leu Arg Pro Ile Phe Leu Glu Ala Asp Gly Val Thr
405 410 415
Pro Gly Pro Tyr Lys Lys Tyr Tyr Asp Ile Leu Thr Trp Val Thr Val
420 425 430
Gln Leu Gly Phe Gly Tyr Met Val Gln Pro Phe Met Ile Leu Glu Phe
435 440 445
Gly Pro Ser Leu Arg Leu Trp Ser Thr Val Tyr Phe Cys Val His Leu
450 455 460
Phe Ile Ala Leu Val Ile Leu Leu Phe Tyr Gly Pro Tyr Lys Arg Thr
465 470 475 480
Val Thr Gly Tyr Leu Asn Ser Leu Arg Pro Lys Glu Thr Thr Ile Lys
485 490 495
Pro Ala Asp Lys Leu Lys Met Asp Ala Glu Lys Leu Arg Gln Leu Gln
500 505 510
His Glu Leu Arg Val Leu Ser Ser Asn Glu Pro Ser Leu Gly Val Pro
515 520 525
Gln Pro Asp Phe Glu Asp Phe Asp Asp Asp Val Lys Glu Ala Ile Ala
530 535 540
Glu Phe Glu Ala Leu Lys Asn Glu Ile Ala Arg Asp Ile Asp Ala Leu
545 550 555 560
Arg Pro Lys Val Asp Lys Glu Pro Leu Lys Asn Ala Lys Pro Gln Ser
565 570 575
Lys Arg Gln
<210> 64
<211> 1740
<212> DNA
<213> 噬腺嘌呤阿克苏拉酵母
<400> 64
atggcgctcg gggtatttat tcagctggcc gaggaggggt ctcggatgac gggaattcct 60
cctgatttgc tgcggttggc gttttgtcta gtattctcct acccaggatg tgccattctt 120
aagcggttac ctgataacaa cactcttgtc aaagagctgt tcatcatgtc agtgtctctt 180
ttctacttgc ttggagtatt cagcatgtgg ggcggagtga gaacgctgct tatcagcaca 240
ttggcgacct attatatcac aaaaaagtgg ccgtcttccc cgtttatgcc ttgggctaac 300
ttccttttcg tcatggcaca cttgttcaca aaccacattg ccaatcagat taaagaggct 360
ggcgaattgt atgaccccaa tgtcattgac atcaccgggg cacagatggt gctttgtatg 420
aagctttcgg catttgggtg gaacgtgtac gacggcacac agccacaggg gtcgctgtcg 480
gatttccaaa agatgcgggc tgtcaagaag cacccatctc tattagattt tgtcacgtat 540
gcttttttct ttccttcagt gcttacgggc ccttcgtttg actacgaaga gtttaggcaa 600
tggattgacc tgtcaatgtt tgacgttact gctaatgatc ccaagcgagg acgggccgtc 660
aatcgtaaaa ttccccgtag tggaagagta gccaccctca aagcactgga gggagtgtta 720
tggattgtgg tgtgggttct agtcacgtcc tactttaatc tcgactatgc gctgtcgcca 780
aagtttacct ccgagctcaa tttcgtcttg aaaatgctat acctctatgt tctgggattc 840
tcgtacagac tcaagtatta tggtgcttgg tccatttccg agggctcgtg catcttggcc 900
ggcattgggt tcaacggaaa gacaaaatct ggaaagtaca aatgggatcg tgttaagaac 960
attgaccctt ggaagtttga gtttggccaa aacacgttta cccttctgga ggcttggaat 1020
atgaatacaa acaagtggct caagaattat gtctatttgc gagtcactcc aaagggtaaa 1080
aagccaggct tccgtagtac tctggccacc ttttttacgt cggccttttg gcatggtacc 1140
aggcctgggt attacttgac gtttgtgact ggtgcttttt tccaggccct aggtaaaata 1200
tttagacgca acctccgtcc aattttcctg gaagctgatg gagttacccc tggaccctat 1260
aaaaagtact acgatattct gacttgggtc acggtgcagc taggctttgg atacatggtt 1320
caaccgttta tgatcctaga gtttggaccc tctcttagac tttggtctac ggtctacttt 1380
tgcgtccacc ttttcatcgc tcttgtcatt cttctctttt atggaccgta caagcgtact 1440
gttaccggat acctcaattc tttacgaccg aaggagacca ccattaagcc cgccgacaag 1500
ctcaagatgg atgctgaaaa gcttcgccag ctccaacacg agctacgagt gttgtcttca 1560
aacgagcctt ctttgggagt tcctcagccc gattttgaag attttgacga cgacgtcaag 1620
gaagcaattg ccgaatttga agcgctcaag aatgaaattg ctcgggacat tgacgccctg 1680
cgaccaaagg tcgacaagga gcctctcaag aacgcaaagc cacagagcaa acgacagtaa 1740
<210> 65
<211> 512
<212> PRT
<213> 解脂耶氏酵母
<400> 65
Met Ala Phe Pro Trp Ala Asp Lys Trp Ala Ala Asp Ala Ser Ala Ser
1 5 10 15
Thr Gly Leu Pro Pro Asp Leu Leu Lys Ile Ala Phe Thr Leu Val Met
20 25 30
Ser Tyr Pro Leu Ser Ser Leu Met Lys Arg Leu Pro Asp Asp Ala Lys
35 40 45
Asn Leu Lys Ile Ile Tyr Ile Ile Ser Val Ser Ile Phe Tyr Met Val
50 55 60
Gly Val Phe Ser Leu Tyr Gly Gly Ala Ala Thr Leu Leu Phe Ser Ser
65 70 75 80
Met Gly Thr Phe Phe Ile Thr Gln Trp Lys Ser Pro Tyr Met Pro Trp
85 90 95
Val Asn Phe Gly Phe Val Met Thr His Leu Phe Val Asn His Leu Arg
100 105 110
Ser Gln Phe Phe Pro Glu Thr Tyr Asp Pro Asn Val Ile Asp Ile Thr
115 120 125
Gly Ala Gln Met Val Leu Cys Met Lys Leu Ser Ser Phe Gly Trp Asn
130 135 140
Val Tyr Asp Gly Trp Gln Ile Glu Lys Gly Glu Gln Leu Ser Glu Phe
145 150 155 160
Gln Thr Lys Arg Ala Val Leu Lys His Pro Ser Leu Met Asp Phe Leu
165 170 175
Ala Phe Val Phe Tyr Phe Pro Ser Ile Leu Thr Gly Pro Ser Tyr Asp
180 185 190
Tyr Met Glu Phe His Asn Trp Leu Asp Leu Ser Leu Phe Lys Glu Leu
195 200 205
Glu Lys Asp Lys Asp Pro Lys Arg Ala Ala Arg Arg Lys Arg His Lys
210 215 220
Ile Pro Arg Ser Gly Ile Ala Ala Ser Lys Lys Leu Ala Ala Gly Ile
225 230 235 240
Phe Trp Ile Val Leu Trp Thr Gln Val Asp Ser Arg Ile Ser Thr Ala
245 250 255
Tyr Ala Tyr Ser Asp Ala Phe Thr Lys Glu His Asn Ile Phe Gly Arg
260 265 270
Ile Val Tyr Leu Tyr Met Leu Gly Phe Met Tyr Arg Leu Lys Tyr Tyr
275 280 285
Gly Ala Trp Ser Ile Ser Glu Gly Ala Cys Ile Leu Ser Gly Leu Gly
290 295 300
Phe His Gly Val Asp Pro Lys Thr Gly Lys Tyr Lys Trp Asp Arg Val
305 310 315 320
Gln Asn Val Asp Pro Trp Gly Phe Glu Thr Gly Gln Asn Thr Lys Ala
325 330 335
Leu Leu Glu Ala Trp Asn Gln Asn Thr Asn Lys Trp Leu Arg Asn Tyr
340 345 350
Val Tyr Leu Arg Val Val Pro Lys Gly Gln Lys Pro Gly Phe Arg Ala
355 360 365
Thr Ile Phe Thr Phe Val Val Ser Ala Phe Trp His Gly Thr Arg Pro
370 375 380
Gly Tyr Tyr Leu Thr Phe Val Thr Ala Ala Met Tyr Gln Ser Val Gly
385 390 395 400
Lys Phe Phe Arg Arg Tyr Leu Arg Pro Phe Phe Met Glu Ser Asp Gly
405 410 415
Lys Thr Ala Gly Pro Tyr Lys Ile Tyr Tyr Asp Ile Val Cys Trp Ile
420 425 430
Val Val Gln Thr Ala Phe Gly Tyr Ala Thr Gln Ser Phe Met Ile Leu
435 440 445
Asp Phe Trp Leu Ser Leu Lys Cys Trp Lys Asn Ser Trp Phe Leu Tyr
450 455 460
His Ile Ala Leu Gly Ala Ile Phe Ala Ile Ser Ser Pro Tyr Lys Ala
465 470 475 480
Trp Ala Ile Pro Lys Ile Lys Lys Lys Gln Ala Gly Ala Val Thr Asp
485 490 495
Lys Lys Asp Ala Lys Glu Glu Val Lys Lys Asp Thr Ile Lys Thr Lys
500 505 510
<210> 66
<211> 1539
<212> DNA
<213> 解脂耶氏酵母
<400> 66
atggcctttc catgggcaga taagtgggca gccgatgcgt ctgcatctac agggctgcct 60
ccggacctcc tcaagattgc attcactctg gtcatgtctt atccgctgag ttctctcatg 120
aaacggctgc cagatgacgc caaaaacctc aagatcatct atatcatctc cgtgtccatc 180
ttctacatgg tgggtgtctt ctccctctat ggcggagctg ccactctgct cttctcctca 240
atgggtacct tcttcatcac ccaatggaag agcccttaca tgccctgggt caattttggt 300
tttgtcatga cccatctctt cgtcaatcac ctgcgttcgc agtttttccc cgaaacatac 360
gaccccaatg tcattgacat caccggagca cagatggttc tgtgtatgaa gctatcgtct 420
tttggatgga acgtctacga tggatggcag attgagaagg gtgagcagct cagcgagttc 480
cagactaaaa gggctgttct caagcacccc agtcttatgg acttcctagc ttttgtgttc 540
tacttccctt ccattctgac aggtccttct tacgactata tggagttcca taactggctc 600
gatctcagcc tgttcaagga gctggagaaa gataaggacc ccaagcgagc tgctcgacga 660
aagcgacaca agatcccccg atctggaatc gctgcttcca agaaactcgc cgctggtatc 720
ttctggatcg ttctgtggac ccaggtggac tctcgaatct ccaccgccta cgcttactca 780
gacgcattca ccaaggagca caacatcttt ggacgaattg tgtacctcta catgctcggt 840
ttcatgtacc gactcaagta ctacggagcc tggtccattt ccgagggagc ctgcatcttg 900
tctggcctcg gattccatgg cgtggacccc aaaactggca agtacaagtg ggaccgtgtc 960
cagaacgtgg acccgtgggg attcgaaact ggtcaaaaca caaaggctct gctggaggcc 1020
tggaaccaga acactaacaa gtggctacga aactatgtgt acctccgagt ggtgcccaaa 1080
ggccaaaagc ctggattccg agccactatc ttcacatttg tggtttccgc cttctggcat 1140
ggaactcgac ctggctacta tctcaccttt gtgaccgctg ccatgtacca gtctgttggt 1200
aagttcttcc gacgatacct gcgacccttc ttcatggagt ctgatggaaa gactgccggt 1260
ccctataaga tctactacga cattgtgtgt tggatcgttg tccaaaccgc atttggatac 1320
gctacccagt cctttatgat tctagacttc tggctgtcgc tcaagtgttg gaagaactcc 1380
tggttcctgt accacattgc tctgggcgcc atctttgcaa tttctagccc ctacaaggca 1440
tgggcgattc ccaagatcaa gaaaaagcag gctggagccg tcactgacaa gaaggacgcc 1500
aaggaggagg tgaagaagga caccatcaag accaagtaa 1539
<210> 67
<211> 346
<212> PRT
<213> 圆红冬孢酵母
<400> 67
Met Pro Ser Pro Leu Ser Arg Trp Leu Pro Val Ile Ala Phe Trp Thr
1 5 10 15
Phe Pro Leu Leu Ala Met Leu Ser Val Ser Pro Ser Leu Arg Thr Phe
20 25 30
Leu Leu Ser Arg Pro Thr Leu Thr Tyr Ala Leu Ala Ser Val Leu Pro
35 40 45
Thr Ile Met Leu Ser Arg Phe Val Ala Pro Ile Arg Tyr Tyr Leu Arg
50 55 60
Leu Thr Thr Phe Leu Val Gly Leu Ala Ala Asn Ala Met Phe Gly Ala
65 70 75 80
Ile Met Ala Leu Pro Met Ser Leu Val Gly Lys Gly Lys Asp Asn Gln
85 90 95
Trp Leu Val Ala Arg Ser Phe Val Asn Thr Val Ala Pro Leu Val Gly
100 105 110
Val Lys Phe Arg Val Glu Gly Arg Glu Asn Leu Asp Lys Ala Asn Pro
115 120 125
Ala Val Leu Val Gly Asn His Gln Thr Met Val Asp Ile Leu Tyr Leu
130 135 140
Gly Ala Val Phe Pro Lys Gly Thr Ser Val Met Ala Lys Arg Glu Leu
145 150 155 160
Gln Trp Thr Pro Ile Leu Gly Gln Trp Met Thr Leu Ser Lys Ala Val
165 170 175
Phe Val Asn Arg Ala Lys Arg Glu Asp Ala Val Lys Val Phe Ala Lys
180 185 190
Val Ala Ala Lys Met Lys Lys Asn Ser Leu Ser Leu Trp Ile Phe Ala
195 200 205
Glu Gly Thr Arg Ser Ala Ser Pro Thr Pro Ser Leu Leu Pro Phe Lys
210 215 220
Lys Gly Ala Phe His Leu Ala Val Gln Ala Gly Leu Pro Val Val Pro
225 230 235 240
Ile Val Cys Glu Asn Tyr Ala His Val Tyr His Ala Lys Ala Arg Arg
245 250 255
Phe Asn Asp Gly Glu Ile Val Val Arg Val Leu Glu Pro Ile Ser Thr
260 265 270
Glu Gly Tyr Thr Ser Ser Ser Ala Asp Ile Ala His Leu Thr Glu Leu
275 280 285
Thr Arg Asp Arg Met Leu Glu Ala Ile Glu Asp Leu Gly Arg Lys Arg
290 295 300
Gln Glu Gln Leu Arg Leu Ala Gly His Gly Gln Gly Gln Gly Gln Gly
305 310 315 320
Glu Arg Glu Ala Leu Leu Ala Gly Arg Glu Ser Thr Ser Gly Glu Thr
325 330 335
Ala Ser Ala Arg Ile Glu Ala Pro Ser Glu
340 345
<210> 68
<211> 1041
<212> DNA
<213> 圆红冬孢酵母
<400> 68
atgccctcgc ccttgtcccg gtggctcccc gtcatcgcat tctggacgtt ccccctcctc 60
gccatgctca gtgtctctcc ctcgctacgc acattcctcc tctcccgccc aacactcacc 120
tacgcgctcg cctcggtcct accaacaatc atgctgtccc gcttcgtcgc accgataagg 180
tattacctcc gcctgacgac tttcctcgtc ggactcgcgg caaacgccat gtttggcgcg 240
atcatggctc tcccgatgag tctcgtgggc aagggaaagg acaaccagtg gctcgtcgcg 300
aggagctttg tcaatactgt tgcgcccctc gtgggagtca agttccgcgt cgaggggagg 360
gagaatttgg acaaggcgaa cccggcggtg cttgtcggga accaccagac catggttgac 420
atcctctacc tcggcgccgt cttccccaag ggcacgtcag tcatggccaa gcgcgagttg 480
cagtggacgc caatcctcgg ccagtggatg acgctctcca aggcggtgtt tgtcaaccgc 540
gccaagcgcg aggacgcagt caaggtgttt gccaaggtcg ccgcaaagat gaagaagaac 600
agcctctcgc tctggatctt tgccgagggc acccgctcgg cctccccgac cccctcgctc 660
ctcccgttca aaaagggcgc gttccacctc gccgtccagg caggcttgcc cgtcgtgcca 720
atcgtgtgcg agaactatgc gcatgtgtat catgccaagg cgaggaggtt caacgacgga 780
gagattgtcg tccgggttct cgaacccatc tcgaccgagg gctacacctc gtcgtcggcc 840
gacatcgcgc acctgacaga gctcacgcgc gaccggatgc tcgaggcgat cgaagacctc 900
ggtcgcaagc ggcaggagca gttgcggctc gccggtcacg gccaaggcca agggcagggc 960
gagcgagagg cactcctcgc gggacgggag agtacgagcg gcgagacggc gagtgcgagg 1020
attgaggcgc cgtccgaata a 1041
<210> 69
<211> 326
<212> PRT
<213> 禾本红酵母
<400> 69
Met Leu Ser Pro Ser Ser Ser Ser Ala Leu Thr Ser Ser Pro Ala Thr
1 5 10 15
Leu Val Pro Thr Leu Ala Val Gly Ala Leu Leu Pro Ala Met Ile Leu
20 25 30
Ala Arg Val Phe Ser Pro Val Arg Tyr Tyr Ala Lys Leu Val Thr Phe
35 40 45
Leu Val Gly Ser Ala Ser Ala Ser Ala Phe Gly Val Ala Ser Ser Leu
50 55 60
Val Met Ala Val Leu Gly Arg Arg Glu Asp Ile Gln Trp Leu Val Ala
65 70 75 80
Arg Phe Leu Lys Tyr Thr Thr Ala Pro Leu Leu Gly Val Lys Phe Arg
85 90 95
Val Glu Gly Ala Asp Lys Leu Glu Thr Thr His Pro Ala Val Leu Val
100 105 110
Gly Asn His Gln Thr Met Leu Asp Leu Leu Tyr Leu Gly Ala Ile Phe
115 120 125
Pro Pro Arg Thr Thr Ile Met Ala Lys Arg Glu Leu Gln Trp Val Pro
130 135 140
Ile Leu Gly Gln Phe Met Thr Leu Ala Lys Ala Ile Phe Val Asn Arg
145 150 155 160
Val Lys Arg Glu Asp Ala Ile Arg Val Phe Asp Gln Val Ala Lys Glu
165 170 175
Met Lys Arg Arg Glu Leu Ser Leu Phe Ile Phe Pro Glu Gly Thr Arg
180 185 190
Ser Ala Ser Ala Ala Pro Ser Leu Leu Pro Phe Lys Lys Gly Ala Phe
195 200 205
His Leu Ala Val Gln Ala Gly Leu Pro Ile Val Pro Ile Val Cys Glu
210 215 220
Asn Tyr Ala His Ile Tyr Tyr Ser Lys Ala Arg Arg Phe Asn Ala Gly
225 230 235 240
Glu Ile Val Val Arg Val Leu Asp Pro Ile Ser Thr Glu Gly Val Thr
245 250 255
Ser Ser His Glu Asp Ile Thr Ala Leu Ile Glu Arg Thr Arg Asn Ala
260 265 270
Met Leu Asp Ala Ile His Gln Leu Gly Arg Glu Arg Arg Ala Gln Leu
275 280 285
Gly Gly Pro Glu Gly Glu Arg Glu Ala Leu Leu Pro Pro Thr Pro Arg
290 295 300
Ala Ser Ile Gly Gly Thr Glu Thr Glu Gly Glu Thr Ala Ser Ala Arg
305 310 315 320
Leu Pro Glu Gly Thr Gln
325
<210> 70
<211> 981
<212> DNA
<213> 禾本红酵母
<400> 70
atgctctcgc cctcctcgtc ctccgccctc acctcgtcgc ccgcgaccct cgtcccgacc 60
ctcgccgtcg gggccctcct gcccgccatg atcctcgccc gcgtcttctc gcccgtgcgg 120
tactacgcca agctcgtcac cttcctcgtc ggctcggcga gcgcctctgc gtttggcgtc 180
gcgtcgtccc ttgtcatggc cgtcctcggc cgccgcgagg acatccagtg gctcgtcgca 240
cggttcctca agtacacgac cgcgcccctc ctcggcgtca agttccgcgt cgagggcgcc 300
gacaagctcg agacgaccca cccggccgtc ctcgtcggca accaccagac catgctcgac 360
ctcctgtacc tcggcgccat cttcccgccc cggacgacca tcatggccaa gcgcgagctg 420
cagtgggtgc ccatcctcgg ccagttcatg acgctcgcaa aggccatctt tgtcaaccgc 480
gtcaagcgcg aggacgccat ccgcgtgttc gaccaggtcg caaaggagat gaagcggcgc 540
gagctgtcgc tcttcatctt ccccgagggc actcgctcgg cgtcggcggc gccgtcgctc 600
ctcccgttca agaagggcgc gttccacctc gccgtccagg ccggcctccc catcgtgccc 660
atcgtgtgcg agaactacgc ccacatctac tactcgaagg ccaggcgctt caacgcgggc 720
gagatcgtcg tgcgggtgct cgacccgatc tcgaccgagg gcgtcacgtc gtcgcacgaa 780
gacatcacgg ccctgatcga gcgcacgcgc aacgccatgc tcgacgccat tcaccagctc 840
gggcgcgagc gtcgcgcgca gctcggcggg cccgagggtg agcgcgaggc gctgctgccg 900
cctacgccga gggcatcgat tggcggtacc gagaccgagg gcgagacggc gagcgcgcgg 960
ttgccggaag ggacgcagta a 981
<210> 71
<211> 399
<212> PRT
<213> 三角褐指藻
<400> 71
Met Arg His Leu Arg Gly Val Leu Ile Thr Leu Ile Leu Asp Trp Val
1 5 10 15
Ser Leu Ser Arg Ser Leu Thr Thr His Ala Pro Tyr Arg Arg Thr Thr
20 25 30
Ala Thr Arg Ser Thr Cys Thr Ala Arg Gln Thr Arg Gly Ser Gln Leu
35 40 45
Ala Ser Thr Thr Pro Phe Asp Arg Asp Gln Ser Glu Ser Phe Ile Val
50 55 60
Pro Asn Glu Asp Val Asn Pro Ile Ile Arg Leu Gly Lys Asp Glu Gln
65 70 75 80
Glu Lys Ile Val Asn Gly Phe Gly Leu Trp Cys Ala Ala Val Ser Val
85 90 95
Phe Thr Gly Pro Leu Trp Val Ala Ala Met Ser Thr Leu Gln Ala Ile
100 105 110
Tyr Lys Ile Asn Ala Asp Trp Asp Pro Asn Arg Ala Leu Tyr Asp Lys
115 120 125
Thr Gly Lys Ile Trp Ser Lys Thr Trp Leu Thr Leu Thr Asp Ser Tyr
130 135 140
Pro Thr Phe Ser Gly Asp Val Asp Arg Leu Lys Ser Ser Gln Gly Pro
145 150 155 160
Cys Leu Tyr Val Ala Asn His Ala Ser Trp Leu Asp Ile Pro Val Ile
165 170 175
Cys Thr Val Leu Asp Pro Val Phe Lys Phe Ile Ala Lys Gly Glu Leu
180 185 190
Arg Lys Val Pro Cys Ile Gly Gln Gln Leu Glu Gly Gly Asn His Ile
195 200 205
Leu Ile Asp Arg Glu Asp Arg Arg Ser Gln Leu Arg Thr Phe Lys Asp
210 215 220
Gly Ile Gly Trp Leu Lys Lys Gly Val Pro Ile Met Ala Phe Pro Glu
225 230 235 240
Gly Met Arg Ser Arg Asp Gly Lys Leu Met Asp Phe Lys Gly Gly Leu
245 250 255
Phe Ser Met Ala Val Lys Thr Gln Val Pro Ile Val Pro Ile Thr Ile
260 265 270
Ser His Thr His Ala Val Met Pro Ser Asn Ala Leu Phe Pro Val Gln
275 280 285
Thr Gly Ala Gly Lys Leu His Val His Val His Asp Pro Ile Asp Thr
290 295 300
Thr Gly Lys Thr Glu Ala Glu Leu Gly Ala Leu Val Arg Ala Ser Phe
305 310 315 320
Leu Ser Thr Leu Pro Leu Gly Gln His Pro Lys Pro Val Val Pro Glu
325 330 335
Ile Glu Gln Thr Ala Glu Lys Asp His Lys Thr Ile Pro Ile Thr Pro
340 345 350
Lys Val Gln Asp Thr His His Leu His Gln Gln His Ile Thr Pro Ser
355 360 365
Gln Thr Leu Ser His Tyr Thr Ala Ala Ser Ser Thr Ile Asn Ser Ser
370 375 380
Gln Glu Val Thr Ser Lys Asn Arg Thr Glu Glu Thr Thr Val Pro
385 390 395
<210> 72
<211> 1200
<212> DNA
<213> 三角褐指藻
<400> 72
atgaggcatt tgagaggcgt actaattaca ctgattctag attgggtttc tttatcgcga 60
tcgttgacga cacacgctcc gtacaggcgt accacagcta cacgttccac ctgtactgct 120
cggcaaaccc ggggatccca attggcatcc accacacctt tcgatcgcga tcagagtgaa 180
agctttattg ttcccaacga agacgttaac ccgattattc ggcttggaaa ggatgagcag 240
gaaaaaattg tgaacggatt tggcctttgg tgcgcagctg tttccgtctt tacggggccc 300
ttgtgggtcg ctgccatgag tacactacaa gctatttaca aaattaacgc cgactgggat 360
ccgaatcgag cactctacga caaaacgggc aaaatttggt ccaaaacctg gctcacactg 420
acagattcct atccgacctt ttccggcgat gtggaccgct taaaaagttc tcaaggccca 480
tgtctatacg tggcgaatca cgcatcttgg ctggacattc cggtgatctg caccgtttta 540
gatccagttt tcaagtttat tgccaaggga gaattgcgca aagtgccatg tattggacaa 600
caactagagg ggggaaatca cattctcatc gatcgagagg accgtcggtc gcaactgcgc 660
acattcaaag atggtattgg ttggctgaaa aagggcgtac caatcatggc ctttcccgaa 720
ggtatgcgct cccgcgatgg aaaactcatg gatttcaaag gagggctctt ttcgatggcg 780
gtaaaaacac aggtgcccat tgttccaatc acgatttcgc acacgcacgc cgtcatgccg 840
tccaacgcct tgtttcccgt gcaaacagga gccggcaaac ttcacgtaca tgttcacgat 900
cccatcgaca cgactggcaa gaccgaagcc gaactaggag cactggtacg ggcatccttt 960
ttgtccacct taccgttggg acagcatcca aagccagtag taccagaaat tgagcaaaca 1020
gccgaaaaag accataagac gattccgatt actcccaaag tgcaagatac acaccacctt 1080
catcaacagc atattacacc cagtcagacc ttatcgcact acacagcagc ctcttctaca 1140
ataaattcca gccaggaagt tacatcaaag aatcggacgg aggagactac tgtaccataa 1200
<210> 73
<211> 262
<212> PRT
<213> 小红酵母
<400> 73
Met Asn Ala Thr Leu Arg Tyr Tyr Tyr Arg Phe Thr Leu Tyr Cys Thr
1 5 10 15
Thr Leu Gly Leu Thr Ser Ala Trp Ala Val Leu Val Ser Ile Ala Leu
20 25 30
Ser Ile Phe Gly Gln Asn His Ser Ile Gln His Tyr Val Ala Arg Ser
35 40 45
Phe Tyr Tyr Phe Ala Ser Pro Ile Val Gly Trp Lys Ile Lys Val Glu
50 55 60
Gly Glu Glu Tyr Leu Lys Asn Glu Gln Ser Thr Val Phe Val Gly Asn
65 70 75 80
His Gln Ser Met Ile Asp Ile Leu Tyr Leu Gly Arg Met Phe Pro Lys
85 90 95
Arg Cys Thr Val Thr Ala Lys Lys Glu Leu Lys Trp Thr Pro Phe Leu
100 105 110
Gly Gln Phe Met Trp Leu Ser Asn Ala Ile Phe Ile Asn Arg Thr Asn
115 120 125
Arg Ala Asp Ala Leu Lys Thr Phe Gln Lys Ala Ala Glu Asp Met Lys
130 135 140
Arg Arg Ser Met Ser Ile Phe Ile Phe Ala Glu Gly Thr Arg Thr Asn
145 150 155 160
Ser Ala Glu Ile Gly Met Leu Pro Phe Lys Lys Gly Ala Phe His Leu
165 170 175
Ala Val Gln Gly Gly Phe Pro Ile Val Pro Met Val Cys Glu Asn Tyr
180 185 190
Tyr Ser Leu Tyr Ala Ala Gly Ile Lys Arg Phe Glu Ala Gly Glu Leu
195 200 205
Val Leu Lys Val Leu Pro Pro Val Ser Thr Glu Gly Tyr Thr Ser Ser
210 215 220
Ser Glu Asp Ile Ala Lys Leu Ser Asp Lys Val Arg Thr Ala Met Leu
225 230 235 240
Glu Ala Leu Glu Glu Leu Ala Glu Arg Arg Lys Ala Ser Thr Thr Gln
245 250 255
Lys Arg Ile Asn Gln Gln
260
<210> 74
<211> 789
<212> DNA
<213> 小红酵母
<400> 74
atgaatgcta ctctgcggta ctactaccgc tttacgctgt actgcaccac gctgggcctc 60
acttcagcct gggctgttct cgtgtcgata gccctgtcca tctttggcca aaatcactcc 120
atacagcact acgtagctag aagcttttat tactttgcaa gtccgatagt aggatggaag 180
atcaaggttg aaggggagga gtacttgaag aatgaacagt caacggtgtt tgttggcaat 240
catcagtcca tgatcgacat cttgtaccta ggacgcatgt tcccaaagag atgtacagtg 300
acggcaaaga aagagctgaa atggacgccc ttcttaggcc aattcatgtg gctgtcaaac 360
gcaatcttca tcaatcgcac aaaccgagcc gatgcattga agaccttcca aaaagcagca 420
gaggatatga agcgacgcag catgtcaatc ttcatctttg ccgagggtac acggacgaat 480
tcggccgaaa ttgggatgct gcctttcaag aagggagctt tccatctggc tgtgcagggt 540
ggattcccta ttgtacctat ggtctgcgag aactactata gcttatatgc agcgggtatt 600
aagaggtttg aggctggcga actcgttcta aaagtattac cgcccgtctc gacggagggc 660
tacacatcgt catctgaaga cattgcaaaa ctcagtgata aagtgagaac agccatgctt 720
gaagcgctag aagagctcgc agaacgtagg aaggcatcta cgacgcagaa aaggataaac 780
cagcagtag 789
<210> 75
<211> 303
<212> PRT
<213> 酿酒酵母
<400> 75
Met Ser Val Ile Gly Arg Phe Leu Tyr Tyr Leu Arg Ser Val Leu Val
1 5 10 15
Val Leu Ala Leu Ala Gly Cys Gly Phe Tyr Gly Val Ile Ala Ser Ile
20 25 30
Leu Cys Thr Leu Ile Gly Lys Gln His Leu Ala Gln Trp Ile Thr Ala
35 40 45
Arg Cys Phe Tyr His Val Met Lys Leu Met Leu Gly Leu Asp Val Lys
50 55 60
Val Val Gly Glu Glu Asn Leu Ala Lys Lys Pro Tyr Ile Met Ile Ala
65 70 75 80
Asn His Gln Ser Thr Leu Asp Ile Phe Met Leu Gly Arg Ile Phe Pro
85 90 95
Pro Gly Cys Thr Val Thr Ala Lys Lys Ser Leu Lys Tyr Val Pro Phe
100 105 110
Leu Gly Trp Phe Met Ala Leu Ser Gly Thr Tyr Phe Leu Asp Arg Ser
115 120 125
Lys Arg Gln Glu Ala Ile Asp Thr Leu Asn Lys Gly Leu Glu Asn Val
130 135 140
Lys Lys Asn Lys Arg Ala Leu Trp Val Phe Pro Glu Gly Thr Arg Ser
145 150 155 160
Tyr Thr Ser Glu Leu Thr Met Leu Pro Phe Lys Lys Gly Ala Phe His
165 170 175
Leu Ala Gln Gln Gly Lys Ile Pro Ile Val Pro Val Val Val Ser Asn
180 185 190
Thr Ser Thr Leu Val Ser Pro Lys Tyr Gly Val Phe Asn Arg Gly Cys
195 200 205
Met Ile Val Arg Ile Leu Lys Pro Ile Ser Thr Glu Asn Leu Thr Lys
210 215 220
Asp Lys Ile Gly Glu Phe Ala Glu Lys Val Arg Asp Gln Met Val Asp
225 230 235 240
Thr Leu Lys Glu Ile Gly Tyr Ser Pro Ala Ile Asn Asp Thr Thr Leu
245 250 255
Pro Pro Gln Ala Ile Glu Tyr Ala Ala Leu Gln His Asp Lys Lys Val
260 265 270
Asn Lys Lys Ile Lys Asn Glu Pro Val Pro Ser Val Ser Ile Ser Asn
275 280 285
Asp Val Asn Thr His Asn Glu Gly Ser Ser Val Lys Lys Met His
290 295 300
<210> 76
<211> 912
<212> DNA
<213> 酿酒酵母
<400> 76
atgagtgtga taggtaggtt cttgtattac ttgaggtccg tgttggtcgt actggcgctt 60
gcaggctgtg gcttttacgg tgtaatcgcc tctatccttt gcacgttaat cggtaagcaa 120
catttggctc agtggattac tgcgcgttgt ttttaccatg tcatgaaatt gatgcttggc 180
cttgacgtca aggtcgttgg cgaggagaat ttggccaaga agccatatat tatgattgcc 240
aatcaccaat ccaccttgga tatcttcatg ttaggtagga ttttcccccc tggttgcaca 300
gttactgcca agaagtcttt gaaatacgtc ccctttctgg gttggttcat ggctttgagt 360
ggtacatatt tcttagacag atctaaaagg caagaagcca ttgacacctt gaataaaggt 420
ttagaaaatg ttaagaaaaa caagcgtgct ctatgggttt ttcctgaggg taccaggtct 480
tacacgagtg agctgacaat gttgcctttc aagaagggtg ctttccattt ggcacaacag 540
ggtaagatcc ccattgttcc agtggttgtt tccaatacca gtactttagt aagtcctaaa 600
tatggggtct tcaacagagg ctgtatgatt gttagaattt taaaacctat ttcaaccgag 660
aacttaacaa aggacaaaat tggtgaattt gctgaaaaag ttagagatca aatggttgac 720
actttgaagg agattggcta ctctcccgcc atcaacgata caaccctccc accacaagct 780
attgagtatg ccgctcttca acatgacaag aaagtgaaca agaaaatcaa gaatgagcct 840
gtgccttctg tcagcattag caacgatgtc aatacccata acgaaggttc atctgtaaaa 900
aagatgcatt aa 912
<210> 77
<211> 619
<212> PRT
<213> 酿酒酵母
<400> 77
Met Tyr Asn Pro Val Asp Ala Val Leu Thr Lys Ile Ile Thr Asn Tyr
1 5 10 15
Gly Ile Asp Ser Phe Thr Leu Arg Tyr Ala Ile Cys Leu Leu Gly Ser
20 25 30
Phe Pro Leu Asn Ala Ile Leu Lys Arg Ile Pro Glu Lys Arg Ile Gly
35 40 45
Leu Lys Cys Cys Phe Ile Ile Ser Met Ser Met Phe Tyr Leu Phe Gly
50 55 60
Val Leu Asn Leu Val Ser Gly Phe Arg Thr Leu Phe Ile Ser Thr Met
65 70 75 80
Phe Thr Tyr Leu Ile Ser Arg Phe Tyr Arg Ser Lys Phe Met Pro His
85 90 95
Leu Asn Phe Met Phe Val Met Gly His Leu Ala Ile Asn His Ile His
100 105 110
Ala Gln Phe Leu Asn Glu Gln Thr Gln Thr Thr Val Asp Ile Thr Ser
115 120 125
Ser Gln Met Val Leu Ala Met Lys Leu Thr Ser Phe Ala Trp Ser Tyr
130 135 140
Tyr Asp Gly Ser Cys Thr Ser Glu Ser Asp Phe Lys Asp Leu Thr Glu
145 150 155 160
His Gln Lys Ser Arg Ala Val Arg Gly His Pro Pro Leu Leu Lys Phe
165 170 175
Leu Ala Tyr Ala Phe Phe Tyr Ser Thr Leu Leu Thr Gly Pro Ser Phe
180 185 190
Asp Tyr Ala Asp Phe Asp Ser Trp Leu Asn Cys Glu Met Phe Arg Asp
195 200 205
Leu Pro Glu Ser Lys Lys Pro Met Arg Arg His His Pro Gly Glu Arg
210 215 220
Arg Gln Ile Pro Lys Asn Gly Lys Leu Ala Leu Trp Lys Val Val Gln
225 230 235 240
Gly Leu Ala Trp Met Ile Leu Ser Thr Leu Gly Met Lys His Phe Pro
245 250 255
Val Lys Tyr Val Leu Asp Lys Asp Gly Phe Pro Thr Arg Ser Phe Ile
260 265 270
Phe Arg Ile His Tyr Leu Phe Leu Leu Gly Phe Ile His Arg Phe Lys
275 280 285
Tyr Tyr Ala Ala Trp Thr Ile Ser Glu Gly Ser Cys Ile Leu Cys Gly
290 295 300
Leu Gly Tyr Asn Gly Tyr Asp Ser Lys Thr Gln Lys Ile Arg Trp Asp
305 310 315 320
Arg Val Arg Asn Ile Asp Ile Trp Thr Val Glu Thr Ala Gln Asn Thr
325 330 335
Arg Glu Met Leu Glu Ala Trp Asn Met Asn Thr Asn Lys Trp Leu Lys
340 345 350
Tyr Ser Val Tyr Leu Arg Val Thr Lys Lys Gly Lys Lys Pro Gly Phe
355 360 365
Arg Ser Thr Leu Phe Thr Phe Leu Thr Ser Ala Phe Trp His Gly Thr
370 375 380
Arg Pro Gly Tyr Tyr Leu Thr Phe Ala Thr Gly Ala Leu Tyr Gln Thr
385 390 395 400
Cys Gly Lys Ile Tyr Arg Arg Asn Phe Arg Pro Ile Phe Leu Arg Glu
405 410 415
Asp Gly Val Thr Pro Leu Pro Ser Lys Lys Ile Tyr Asp Leu Val Gly
420 425 430
Ile Tyr Ala Ile Lys Leu Ala Phe Gly Tyr Met Val Gln Pro Phe Ile
435 440 445
Ile Leu Asp Leu Lys Pro Ser Leu Met Val Trp Gly Ser Val Tyr Phe
450 455 460
Tyr Val His Ile Ile Val Ala Phe Ser Phe Phe Leu Phe Arg Gly Pro
465 470 475 480
Tyr Ala Lys Gln Val Thr Glu Phe Phe Lys Ser Lys Gln Pro Lys Glu
485 490 495
Ile Phe Ile Arg Lys Gln Lys Lys Leu Glu Lys Asp Ile Ser Ala Ser
500 505 510
Ser Pro Asn Leu Gly Gly Ile Leu Lys Ala Lys Ile Glu His Glu Lys
515 520 525
Gly Lys Thr Ala Glu Glu Glu Glu Met Asn Leu Gly Ile Pro Pro Ile
530 535 540
Glu Leu Glu Lys Trp Asp Asn Ala Lys Glu Asp Trp Glu Asp Phe Cys
545 550 555 560
Lys Asp Tyr Lys Glu Trp Arg Asn Lys Asn Gly Leu Glu Ile Glu Glu
565 570 575
Glu Asn Leu Ser Lys Ala Phe Glu Arg Phe Lys Gln Glu Phe Ser Asn
580 585 590
Ala Ala Ser Gly Ser Gly Glu Arg Val Arg Lys Met Ser Phe Ser Gly
595 600 605
Tyr Ser Pro Lys Pro Ile Ser Lys Lys Glu Glu
610 615
<210> 78
<211> 1860
<212> DNA
<213> 酿酒酵母
<400> 78
atgtacaatc ctgtggacgc tgttttaaca aagataatta ccaactatgg gattgatagt 60
tttacactgc gatatgctat ctgcttattg ggatcgttcc cactgaatgc tattttgaag 120
agaattcccg agaagcgtat aggtttaaaa tgttgtttta tcatttctat gtcgatgttt 180
tacttattcg gtgtgctgaa tctagtaagt ggattcagga ccctgtttat tagtaccatg 240
tttacttact tgatctcaag attttaccgt tccaagttta tgccacactt gaatttcatg 300
tttgttatgg gtcatttggc aataaatcat atacacgccc aattccttaa cgaacagact 360
caaactaccg ttgacattac aagttcacaa atggttttag ccatgaaact aacttctttt 420
gcatggtcgt actatgatgg ttcatgcact agcgaaagcg atttcaaaga tttgactgag 480
catcaaaaat ctcgtgctgt cagaggtcat ccacccttat taaagttcct ggcatatgca 540
tttttctatt caacgttgct aactggccca agtttcgatt atgccgattt tgacagctgg 600
ttgaattgtg agatgttccg tgacttgcct gaaagcaaaa agcctatgag aagacaccac 660
cctggtgaaa gaagacagat tccaaagaat ggtaaacttg cattatggaa agttgttcaa 720
ggtcttgctt ggatgatttt aagtacacta ggaatgaagc acttccccgt aaaatacgtt 780
ttggacaaag atggcttccc aacgagatct tttatattca gaatccatta cttattcttg 840
cttggtttca tccatagatt caagtactac gctgcctgga ctatttcgga aggatcttgt 900
attttgtgcg gtttgggtta taatggttat gattcaaaga cacaaaagat cagatgggat 960
cgtgtcagaa atattgacat ttggaccgta gaaacggcgc agaatacgcg tgaaatgttg 1020
gaagcatgga atatgaatac taacaagtgg ctaaaatact ctgtttattt acgtgtcaca 1080
aagaagggca aaaaacctgg tttccgctca actttgttta ctttcctaac ttccgcattt 1140
tggcatggta ccagacctgg gtactatctg acttttgcga caggggcttt gtaccaaaca 1200
tgtggtaaaa tctacagacg caattttaga ccaattttct tgcgagaaga tggtgtcact 1260
cctttgcctt ctaaaaaaat ctacgattta gttggcatat atgcaattaa actagcattt 1320
ggttacatgg tgcaaccatt tattatcctt gatttgaagc catctttaat ggtatggggc 1380
tctgtttatt tctatgttca tattattgtt gctttctcat ttttcctatt cagaggacca 1440
tatgctaaac aagttactga attttttaaa tccaaacaac ctaaagaaat attcattaga 1500
aaacaaaaga agttggaaaa agatatttct gcaagctctc caaacttggg tggtatattg 1560
aaggcaaaga ttgaacatga aaagggaaag acagcagaag aagaagaaat gaacttaggt 1620
attccaccaa ttgagttaga aaagtgggac aatgctaagg aagattggga agatttctgc 1680
aaagattaca aagaatggag aaataaaaat ggtcttgaaa tagaagagga aaacctttct 1740
aaagcttttg aaagattcaa gcaggaattt tctaacgctg caagtggatc aggtgaacgt 1800
gtgagaaaaa tgagttttag tggttactca ccaaagccta tttcaaaaaa ggaagagtag 1860
<210> 79
<211> 300
<212> PRT
<213> 酿酒酵母
<400> 79
Met Glu Lys Tyr Thr Asn Trp Arg Asp Asn Gly Thr Gly Ile Ala Pro
1 5 10 15
Phe Leu Pro Asn Thr Ile Arg Lys Pro Ser Lys Val Met Thr Ala Cys
20 25 30
Leu Leu Gly Ile Leu Gly Val Lys Thr Ile Ile Met Leu Pro Leu Ile
35 40 45
Met Leu Tyr Leu Leu Thr Gly Gln Asn Asn Leu Leu Gly Leu Ile Leu
50 55 60
Lys Phe Thr Phe Ser Trp Lys Glu Glu Ile Thr Val Gln Gly Ile Lys
65 70 75 80
Lys Arg Asp Val Arg Lys Ser Lys His Tyr Pro Gln Lys Gly Lys Leu
85 90 95
Tyr Ile Cys Asn Cys Thr Ser Pro Leu Asp Ala Phe Ser Val Val Leu
100 105 110
Leu Ala Gln Gly Pro Val Thr Leu Leu Val Pro Ser Asn Asp Ile Val
115 120 125
Tyr Lys Val Ser Ile Arg Glu Phe Ile Asn Phe Ile Leu Ala Gly Gly
130 135 140
Leu Asp Ile Lys Leu Tyr Gly His Glu Val Ala Glu Leu Ser Gln Leu
145 150 155 160
Gly Asn Thr Val Asn Phe Met Phe Ala Glu Gly Thr Ser Cys Asn Gly
165 170 175
Lys Ser Val Leu Pro Phe Ser Ile Thr Gly Lys Lys Leu Lys Glu Phe
180 185 190
Ile Asp Pro Ser Ile Thr Thr Met Asn Pro Ala Met Ala Lys Thr Lys
195 200 205
Lys Phe Glu Leu Gln Thr Ile Gln Ile Lys Thr Asn Lys Thr Ala Ile
210 215 220
Thr Thr Leu Pro Ile Ser Asn Met Glu Tyr Leu Ser Arg Phe Leu Asn
225 230 235 240
Lys Gly Ile Asn Val Lys Cys Lys Ile Asn Glu Pro Gln Val Leu Ser
245 250 255
Asp Asn Leu Glu Glu Leu Arg Val Ala Leu Asn Gly Gly Asp Lys Tyr
260 265 270
Lys Leu Val Ser Arg Lys Leu Asp Val Glu Ser Lys Arg Asn Phe Val
275 280 285
Lys Glu Tyr Ile Ser Asp Gln Arg Lys Lys Arg Lys
290 295 300
<210> 80
<211> 903
<212> DNA
<213> 酿酒酵母
<400> 80
atggaaaagt acaccaattg gagagacaat ggtacgggaa tagctccatt tctaccaaac 60
acaatcagga aacctagtaa ggtgatgaca gcgtgtttgt tgggtatcct aggggtgaaa 120
accattataa tgctaccatt gattatgctg taccttctaa ctggccagaa caacttactg 180
ggtttgatat tgaagtttac attcagttgg aaagaggaaa ttaccgtgca aggaatcaag 240
aaacgtgacg taaggaaatc caagcattat ccacagaagg gcaagcttta tatttgcaat 300
tgtacctcac ctttagatgc tttttcagtg gtgttattag ctcaagggcc tgttacgttg 360
ttggtcccat ccaatgacat tgtatacaaa gtttccataa gagaattcat caacttcatc 420
ctcgccggtg ggttagatat aaaactctat ggccacgagg tagcagagct atctcaattg 480
ggcaataccg tgaattttat gtttgctgag ggtacctcat gtaatggtaa aagcgtctta 540
ccgtttagta taaccgggaa aaaacttaaa gaattcatag acccttcaat aaccacaatg 600
aaccccgcaa tggccaaaac taaaaaattt gaattgcaga ccatccaaat caaaactaat 660
aaaactgcca tcaccacatt gcccatctcc aatatggagt atttatctag atttctgaac 720
aagggcatta atgttaaatg caagatcaac gagccacaag tactctcgga taatttagag 780
gaattacgcg ttgcattaaa cggtggcgac aaatataaac tagtctcacg gaagttagat 840
gttgaatcta agaggaattt tgtgaaggaa tatatcagcg atcaacgtaa aaagaggaag 900
tag 903
<210> 81
<211> 280
<212> PRT
<213> 噬腺嘌呤阿克苏拉酵母
<400> 81
Met Glu Lys Phe Ser Asn Trp Arg Asp Gly Ala Thr Gly Val Ala Pro
1 5 10 15
Phe Leu Pro Leu Pro Lys Pro Glu Ser Ser Pro Ala Gly Thr Ala Cys
20 25 30
Gly Leu Ile Leu Gly Leu Ile Arg Leu Pro Leu Val Ile Val Leu Thr
35 40 45
Ile Phe His Phe Leu Leu Phe Gln Trp Leu Gly Pro Leu Asn Ala Val
50 55 60
Ser Ile Arg Leu Ile Leu Met Val Leu Gly Val Tyr Arg Val Ser Thr
65 70 75 80
Val Leu Glu Asn Lys Arg Ala Pro Thr Pro Gly Lys Gly Asp Ile Ile
85 90 95
Val Ser Asn Phe Thr Ser Pro Leu Asp Pro Leu Ile Tyr Ser Val Leu
100 105 110
Phe Asp Pro Leu Phe Val Ile Pro Asp Gly Asn Ala Arg Phe Leu Ala
115 120 125
Leu Ser Met Pro Gly Ala Phe Arg Gln Ala Leu Ser Val Pro Thr Ile
130 135 140
Gly Gln Gly Pro Ser Ala Gly His Asp Leu Ala Lys Leu Val Asp Arg
145 150 155 160
Ala Arg Ala Ser Gly Lys Leu Leu Val Ile Phe Ala Glu Gly Thr Thr
165 170 175
Thr Asn Gly Arg Gly Leu Leu Ala Ile Pro Asp Lys Gly Thr Pro Ile
180 185 190
Ser Asn Thr Ala Thr Gly Arg Val Tyr Ala Ser Ala Val Arg Tyr Asn
195 200 205
Pro Pro Arg Gly Cys Ser Pro Val Pro Gln Thr Ala Val Arg Trp Val
210 215 220
Trp Gly Leu Val Ser Cys Thr Gly Val Asn Cys Thr Val Arg Met Ser
225 230 235 240
Ala Pro Val Glu Gly Lys Gln Thr Val Ala Glu Ser Ile Cys Arg Val
245 250 255
Gly Arg Leu Arg Gln Leu Gly Leu Ser Ala Asn Asp Lys Val Asp Phe
260 265 270
Tyr Gln Ala Trp Lys Lys Tyr His
275 280
<210> 82
<211> 843
<212> DNA
<213> 噬腺嘌呤阿克苏拉酵母
<400> 82
atggagaagt tttccaactg gcgagacggc gctacgggcg tggcaccatt tttaccgttg 60
ccaaagccag aatcttcacc ggcaggcaca gcatgtggac tgattttggg cctgatccgg 120
ctacctctgg tgattgtttt gacgatattt cactttctcc tgtttcaatg gctggggcca 180
ttgaatgcgg tgtcaattcg attgatactc atggtcttag gagtatacag agtttcgaca 240
gtgctcgaga acaaacgtgc accaactcct ggcaaaggag acattatcgt atccaatttc 300
acgtccccat tggaccctct aatctattca gtattgtttg accccctgtt tgtcattcct 360
gatggaaatg cgagatttct ggccctgtca atgccgggtg cgttccgaca agcattaagc 420
gttccaacca tcggtcaggg accatctgca ggacacgacc tggctaagct agtcgatcgc 480
gccagggcat ccggaaagct cttggtgatc ttcgccgaag gcaccaccac gaacggccgg 540
ggcctcctgg caatcccaga caaaggcacc ccaatctcga acaccgcgac tggacgcgta 600
tatgcatcgg cggtacggta taatccccct cgaggatgct ctcctgtgcc ccagaccgcg 660
gtacgatggg tgtggggatt agtgtcttgc actggcgtca attgcaccgt ccggatgtct 720
gctccagtgg agggcaagca aaccgttgcc gagagtatct gtcgggttgg ccgcctccgt 780
cagctcggac tatctgcaaa cgacaaggta gatttttacc aagcttggaa aaagtaccac 840
taa 843
<210> 83
<211> 305
<212> PRT
<213> 解脂耶氏酵母
<400> 83
Met Glu Lys Phe Ser Gln Tyr Arg Asp Lys Gly Thr Gly Val Ala Pro
1 5 10 15
Tyr Leu Pro His Pro Arg Ser Lys Ala Asp Gly Ser Leu Pro Ser Thr
20 25 30
Ile Phe Val Val Leu Gln Ala Pro Leu Ala Leu Val Glu Ser Val Ile
35 40 45
Lys Ile Pro Leu Leu Leu Ala Leu Leu Ala Leu Tyr Ala Gly Ile Ile
50 55 60
Gln Phe Ile Thr Ile Glu Pro Val Arg Lys Ala Tyr Phe Ser Thr Leu
65 70 75 80
Leu Phe Val Ser Gly Phe Trp Phe Trp Asn Val Ser Ala Glu Ala Val
85 90 95
Arg Arg Asn Lys Leu Thr Glu Ala Tyr Pro Lys Pro Gly Glu Val Val
100 105 110
Val Ser Asn Tyr Leu Ser Pro Ile Asp Ala Phe Val Tyr Ser Ala Leu
115 120 125
Phe Asp Pro Val Phe Ile Val Pro His Ala Ser Ser Arg Val Tyr Gln
130 135 140
Glu Leu Gly Pro Phe Gly Val Phe Phe Lys Ala Leu Gly Ile Pro Glu
145 150 155 160
Ile Val Pro Pro Thr His Gly Glu Ser Leu Ser Lys Ile Val Phe Asp
165 170 175
Ala Thr Ser Lys Gly Arg Ala Val Val Val Phe Ala Glu Gly Thr Thr
180 185 190
Ser Asn Gly Arg Gly Leu Leu Pro Leu Leu His Ile Asp Phe His Gln
195 200 205
Leu Ser Gln Asn Thr Lys Val Ile Pro Ala Gly Leu Arg Leu Ala Pro
210 215 220
Gln Tyr Ile Thr Thr Pro Leu Pro Val Thr Leu Pro Met Trp Val Phe
225 230 235 240
Arg Leu Leu Ser Asn Pro Thr Gly Trp His Val Ser Leu Arg Phe Ala
245 250 255
Glu Pro Cys Tyr Ala Lys Asp Thr Asn Val Asn Asn Thr Leu Val Glu
260 265 270
Ser Ile Cys Arg Val Gly Arg Leu Lys Ser Ile Gly Pro Asp Leu Gly
275 280 285
Val Glu Gly Lys Arg Asn Phe Trp Lys Val Tyr Asn Lys Lys Lys Asp
290 295 300
Val
305
<210> 84
<211> 918
<212> DNA
<213> 解脂耶氏酵母
<400> 84
atggaaaagt tctcccagta ccgcgataag ggcacaggtg ttgctcccta cctgccgcat 60
ccgcgctcca aggctgacgg ttcgctcccc agcacgattt tcgtcgttct gcaggcgccc 120
ctggctcttg tggagagtgt gatcaagatc cccctcttgc tcgccctgct ggcgctgtat 180
gcgggcatca tccaattcat cacaatcgag ccggtacgaa aggcctactt ttcgacgctg 240
ctgtttgtca gtggcttctg gttctggaac gtgtccgccg aagcggtgag aagaaacaag 300
ctgaccgagg cctaccccaa gcccggagag gtcgtggtgt ccaactacct gtcgcccatt 360
gacgcctttg tgtactctgc acttttcgac cccgttttca tcgtccccca cgcctcttcc 420
cgagtctacc aggagctggg tccgtttgga gtcttcttca aggccctggg tatccccgaa 480
atcgtgcctc caacccatgg ggagtcgctg tccaagattg tgtttgacgc cacctccaag 540
ggacgagccg tggttgtgtt tgccgaggga acaacctcca acggacgagg tctgctgccc 600
ctgctacaca ttgacttcca ccagctctct caaaacacaa aggtgattcc ggcaggcctc 660
agactcgccc cccagtacat caccacccct ctgcccgtga cgctgcccat gtgggtcttc 720
cgactgctct caaaccccac tggatggcac gtgtctctac ggtttgccga accgtgctac 780
gcgaaggaca ccaacgtcaa caacacactg gtggagagca tttgtcgggt gggccgactc 840
aagagcatcg gtccagatct cggtgttgag ggaaagcgaa acttttggaa ggtctacaac 900
aagaagaagg acgtttga 918
<210> 85
<211> 579
<212> PRT
<213> 噬腺嘌呤阿克苏拉酵母
<400> 85
Met Ile Arg Ala Ala Tyr Gly Ser Val Ser Arg Ala Arg Asp Ser Leu
1 5 10 15
Thr Leu Arg Ala Pro Ser Phe Pro Thr Thr Ala Val Glu Val Arg Asp
20 25 30
Lys Ile Leu Trp Ile Leu Tyr Ala Trp Ile Glu Met Phe Thr Asp Val
35 40 45
Phe Ser Phe Trp Thr Glu Lys Val Trp Gly Tyr Val Ser Thr Pro Thr
50 55 60
Lys Glu Ser Ile Leu Arg Lys Gln Leu Asp Glu Ala Lys Ser Tyr His
65 70 75 80
Glu Trp Glu Glu Leu Ser Tyr Lys Leu Asp Ser Ile Leu Gly Asn Asp
85 90 95
Ile Trp Arg Gln Asn Pro Val Ser Arg Lys Tyr Asp Tyr Arg Leu Ile
100 105 110
Ser Thr Arg Leu Lys Glu Leu Val Ala Ala Arg Asp Asn Arg Asn Ile
115 120 125
Glu Leu Leu Met Asp Arg Leu Arg Ser Gly Leu Leu Arg Asn Ile Gly
130 135 140
Ser Ile Ala Ser Thr His Leu Tyr Asn Arg Ala Tyr Ser Gly Thr Lys
145 150 155 160
Leu Leu Ile Glu Asp Tyr Ile Asn Val Val Ile Gln Cys Leu Glu Tyr
165 170 175
Val Glu Arg Gly Gly Arg Pro Leu Thr Ala Ser Ala Ser Lys Ile Pro
180 185 190
Asn Gly Gly Glu Pro Pro Ser Pro Arg Thr Tyr His Lys Pro Met Ile
195 200 205
Thr Arg Gln Arg Lys Leu Asn Phe Phe Asn Asp Thr Arg Gln Ser Phe
210 215 220
Gly Ser Thr Ala Val Val Leu His Gly Gly Ser Leu Phe Gly Leu Cys
225 230 235 240
His Ile Gly Met Ile Lys Thr Leu Phe Asn Gln Gly Leu Leu Pro Arg
245 250 255
Ile Val Cys Gly Ser Thr Val Gly Ala Leu Val Ala Ser Leu Val Cys
260 265 270
Ser Cys Val Asp Glu Glu Val Tyr Glu Thr Leu Asp Asn Val Ser Ser
275 280 285
Glu Met Ser Pro Leu Arg Gln Gly Tyr Thr Asp Ile Lys Tyr His Ser
290 295 300
Val Ala Glu Gly Val Ile Ser Ser Met Cys Pro Pro Glu Ile Leu Ile
305 310 315 320
Phe Glu Gln Tyr Ile Arg Glu Lys Leu Gly Asp Leu Thr Phe Glu Glu
325 330 335
Ala Tyr Gln Arg Thr Gly Arg Ile Leu Asn Ile Pro Val Thr Pro Lys
340 345 350
Ala Lys Pro Gly Gln Val Ala Pro Pro Val Pro Thr Leu Leu Asn Tyr
355 360 365
Leu Ser Ser Pro Asn Val Val Val Trp Ser Ala Ala Gln Cys Ser Ile
370 375 380
Gly Thr Gly Ile Ile His Lys Lys Val Glu Leu Leu Val Lys Gly Leu
385 390 395 400
Asp Gly Gln Leu Lys Pro Tyr Leu Asp Ala Asp Asp Ile Glu Tyr Thr
405 410 415
Pro Ala Asn Gln Ala Val Tyr Ala Ala Asp Arg Glu Ser Pro Tyr Thr
420 425 430
Arg Leu Ser Glu Leu Phe Asn Val Asn Asn Tyr Ile Val Ser Val Ala
435 440 445
Arg Pro Tyr Phe Ala Pro Ile Leu Leu Ser Asp Phe Lys Tyr Arg Ala
450 455 460
Ala Lys Ser Phe Lys Thr Arg Phe Leu Lys Leu Thr Arg Leu Glu Leu
465 470 475 480
Gln Tyr Arg Leu Asn Gln Leu Ser Gln Leu Gly Leu Val Pro Pro Met
485 490 495
Ile Gln Gln Trp Phe Val Asp Gly Asn Ile Pro Ala Gly Phe Gln Val
500 505 510
Thr Val Val Pro Glu Leu Pro Ser Leu Ile Arg Asp Ile Gly Lys Val
515 520 525
Phe Asp Ser Asp Asn Ile Lys Glu Lys Val Asp Tyr Trp Ile Lys Ile
530 535 540
Gly Glu Arg Ser Val Trp Pro Val Leu Asn Ile Ile Trp Ala Arg Cys
545 550 555 560
Ala Ile Glu Phe Val Leu Asp Asp Leu Tyr His Ser Arg Arg Lys Asp
565 570 575
Glu Leu Asp
<210> 86
<211> 1740
<212> DNA
<213> 噬腺嘌呤阿克苏拉酵母
<400> 86
atgattaggg ctgcctacgg gtcagtgtcc agggcccgag attctttaac gttgagggct 60
ccatcttttc ctaccactgc tgtggaggtc cgtgacaaga ttctatggat tctgtatgcc 120
tggattgaaa tgttcacgga cgtctttagc ttctggacgg agaaggtgtg gggttatgtt 180
tctactccta ctaaagaaag cattcttaga aagcaactcg acgaggcaaa atcataccat 240
gaatgggagg agctcagcta caaactagac tcaattttag ggaacgatat ttggcgacag 300
aaccctgtta gccgaaagta tgactatcgc ctgatttcta cccgcctcaa ggaattggtt 360
gctgctaggg ataatcgcaa cattgaattg ctaatggatc ggctaaggtc aggcctgctt 420
cgtaatattg gatcgattgc aagtactcat ctctacaacc gagcgtattc gggcacaaaa 480
ctgttaattg aggattacat taatgtagtg attcaatgcc tggagtatgt tgaacggggc 540
ggcaggccat tgactgcttc agcatccaag attcccaatg gcggtgaacc cccttctcca 600
cgaacctacc ataagcccat gattaccaga cagcgcaagc tcaacttctt caatgataca 660
cgccagtcgt ttggaagtac agctgtggta cttcacggcg ggtccttgtt tggactttgc 720
catattggca tgattaaaac attgttcaac cagggtctac ttcctcgcat agtctgtggc 780
tccacagtgg gagcactagt agcgagtcta gtatgctcct gtgtggatga agaggtgtat 840
gagactttgg ataatgtgtc ttcggaaatg tctcctctcc gccaaggata cactgatata 900
aagtaccatt cggtagccga aggggtcatt tcatcaatgt gtccgccaga gattttgatt 960
tttgaacagt acatccgaga aaaactcgga gacctgacat ttgaagaagc atatcaacgc 1020
accggccgca ttcttaatat cccagtgaca ccaaaggcaa aaccaggtca ggtagcacca 1080
ccagtcccga cgctcctgaa ttatttgtcg agcccgaatg ttgtagtatg gtcagcagcg 1140
caatgcagca ttggaacggg gattattcac aagaaggttg aacttttagt aaaaggtctg 1200
gatggtcaat taaaacctta tttggatgcg gatgatattg aatacactcc tgcaaatcaa 1260
gctgtatacg ctgctgatcg cgagagtccc tatacaagat tgtctgagct gttcaatgtg 1320
aacaattaca ttgtatcagt agctcgcccc tactttgccc caattctgct ttcggatttc 1380
aagtaccgtg cagctaaaag cttcaagacc cggttcctca aactaacccg tctggagtta 1440
cagtatcgtc tcaatcagct gtctcaattg gggctggttc cgcccatgat tcaacaatgg 1500
tttgtggacg gtaacattcc cgccgggttc caagttaccg tggtgcctga attaccctca 1560
cttattagag acatcggcaa ggttttcgat tcggataata taaaggagaa ggtcgactac 1620
tggattaaga tcggtgagcg cagtgtgtgg ccagtgctga atattatctg ggcaaggtgc 1680
gcaattgagt ttgtgctcga cgatctatat cacagccgac gtaaagacga actcgactag 1740
<210> 87
<211> 633
<212> PRT
<213> 噬腺嘌呤阿克苏拉酵母
<400> 87
Met Asn Pro Phe Asp Val Asp Tyr Thr Asn Arg Asp His Leu Val Asp
1 5 10 15
Phe Glu Arg Ala Leu His Glu Asp Glu Ala Ser His Ile Ile Ser Val
20 25 30
Asn Asp Trp Ala Pro Val His Ala Pro Leu Lys Arg Arg Leu Arg Arg
35 40 45
Lys Pro Thr Asp Ser Asp Pro Gly Thr Gly Leu Gly Tyr Thr Leu Leu
50 55 60
Arg Trp Pro Ile Leu Val Ala Ile Ala Leu Trp Leu Ala Leu Leu Ala
65 70 75 80
Phe Val Tyr Ala Ile Val Arg Phe Trp Val Ala Leu Phe Glu Tyr Phe
85 90 95
Val Thr Trp Arg Gly Pro Arg Arg Asn Leu Arg Glu Lys Leu Arg Ser
100 105 110
Ala Arg Ser Tyr Glu Glu Trp Ile Ser Ala Ala Lys Val Leu Asp Asp
115 120 125
His Leu Gly Asn Thr Ser Trp Lys His Asn Pro Lys Phe Ser Arg Tyr
130 135 140
Asp Tyr Arg Thr Ile Asp Arg Ile Thr Asn Ser Leu Arg Gln Leu Arg
145 150 155 160
Asn Gln Asn Lys Ala Glu Glu Val Gly Ser Ile Leu Gln Gly Cys Val
165 170 175
Lys His Asn Phe Ala Gly Thr Gln Gly Gln Pro Leu Tyr Ser Gln Cys
180 185 190
Tyr Tyr Gly Thr Lys Asp Leu Val Glu Glu Phe Asn Ser Glu Ile Val
195 200 205
Lys Ser Leu Asp Tyr Leu Ala Thr His Pro Asp Leu Ser Pro Gln Ser
210 215 220
Arg Arg Leu Leu Phe Lys Met Phe Ser Lys Asn Phe Gly Lys Thr Ala
225 230 235 240
Leu Cys Leu Ser Gly Gly Ala Thr Phe Ala Tyr Arg His Phe Gly Val
245 250 255
Val Lys Ala Leu Leu Glu Gln Gly Leu Leu Pro Asn Ile Ile Ser Gly
260 265 270
Thr Ser Gly Gly Gly Leu Val Ala Ala Leu Val Gly Thr Arg Thr Asn
275 280 285
Ser Glu Leu Arg Glu Leu Leu Thr Pro Gln Leu Ala Asp Lys Ile Thr
290 295 300
Ala Cys Trp Glu Lys Phe Pro Lys Trp Val Tyr Arg Phe Tyr Ser Thr
305 310 315 320
Gly Ala Arg Phe Asp Ala Val Asp Trp Ala Glu Arg Ser Cys Trp Phe
325 330 335
Thr Leu Gly Ser Leu Thr Phe Arg Glu Ala Tyr Asp Arg Thr Gly Lys
340 345 350
Ile Leu Asn Ile Ser Thr Val Pro Ala Asp Pro Asn Ser Pro Ser Ile
355 360 365
Leu Cys Asn Tyr Ile Thr Ser Pro Asp Cys Val Ile Trp Ser Ala Leu
370 375 380
Leu Ala Ser Ala Ala Val Pro Gly Ile Leu Asn Pro Val Val Leu Met
385 390 395 400
Met Lys Thr Lys Lys Gly Asn Leu Val Pro Tyr Ser Phe Gly Asn Lys
405 410 415
Trp Lys Asp Gly Ser Leu Arg Thr Asp Ile Pro Val His Ala Leu Asn
420 425 430
Val Tyr Phe Asn Val Asn Phe Thr Ile Val Ser Gln Val Asn Pro His
435 440 445
Ile Ser Leu Phe Met Tyr Ala Pro Arg Gly Thr Val Gly Arg Pro Val
450 455 460
Ser His Arg Gln Gly Lys Gly Trp Arg Gly Gly Phe Leu Gly Ser Ala
465 470 475 480
Leu Glu Asp Met Leu Lys Leu Glu Ile Arg Lys Trp Leu Lys Leu Met
485 490 495
Lys Asn Leu Ser Leu Met Pro Arg Phe Phe Asn Gln Asp Trp Ser Ser
500 505 510
Val Trp Leu Gln Thr Phe Glu Gly Ser Val Thr Leu Trp Pro Arg Ile
515 520 525
Arg Leu Lys Asp Phe Tyr Tyr Ile Leu Ser Asp Pro Thr Arg Glu Gln
530 535 540
Met Glu Thr Met Ile Ile Ser Gly Gln Arg Cys Thr Phe Pro Lys Leu
545 550 555 560
Leu Phe Ile Lys His Gln Val Asn Ile Glu Arg Ala Ile Asp Arg Gly
565 570 575
Arg Lys His Asn Ala Lys Ala Arg Glu Glu Asn Gly Pro Gln Leu Arg
580 585 590
Arg Val Asn Pro Phe Leu His Asp Leu Asp Asp Arg Val Tyr His Ser
595 600 605
Ser Ser Ser Val Asp Pro Arg Glu Phe Gln Asp Asp His Asp Asp Glu
610 615 620
Asp Asp Asp Ser Thr Asp Ser Ser Met
625 630
<210> 88
<211> 1902
<212> DNA
<213> 噬腺嘌呤阿克苏拉酵母
<400> 88
atgaacccgt ttgatgtaga ttacacaaac agggaccatc tggtcgactt tgaacgagct 60
ttgcacgaag atgaggcttc ccatattata tcggtaaacg actgggctcc agtgcatgct 120
cctctcaagc gacggttgag acgcaagccg acagattcgg atcctgggac aggattagga 180
tacactttgc ttagatggcc tattctggtg gcaattgcgc tgtggctggc cctgttagca 240
tttgtgtacg ccatagtgag gttttgggtc gctctgtttg agtactttgt tacctggcga 300
ggaccccggc gcaatcttcg tgaaaagcta cgcagcgctc gtagttacga ggaatggatt 360
agtgctgcca aagttcttga tgaccatcta ggaaatactt cttggaagca caacccaaag 420
ttctctcgat acgactaccg tactattgat cgcatcacta actcactgcg gcaactgcga 480
aaccagaaca aggccgagga ggttggctct attctacaag gatgcgtcaa gcacaacttt 540
gctggaactc agggccaacc tttgtactct cagtgctact atggcacaaa ggacctggta 600
gaggagttca attctgaaat tgtgaaatcg ctcgattacc tggcaaccca tccagacctg 660
agtcctcaat ctagacgtct tttgttcaaa atgttttcca agaattttgg aaagacggca 720
ttgtgcctct ctggaggggc aacatttgcc tatagacatt tcggagttgt taaagcgctc 780
ttggaacagg gcttgctgcc taatattatt tctggtactt ctggcggagg attggtagct 840
gcgctagttg gtaccagaac aaatagtgaa ctccgtgagc ttctcactcc tcaactggcc 900
gacaagatca ccgcctgctg ggaaaagttc ccaaaatggg tttatagatt ctacagcacc 960
ggcgctcgat tcgatgccgt cgactgggct gaacggtctt gctggtttac actaggaagc 1020
ctgactttta gagaggccta cgatcgaact ggaaagatcc tcaacatttc cactgttcct 1080
gctgacccta attccccttc aatcctctgc aattacatta cttctcccga ctgtgtcatc 1140
tggtcggctt tacttgcttc tgctgcagta ccgggaattc tgaacccagt ggtgctcatg 1200
atgaagacga aaaagggcaa tctggtacct tacagctttg gtaacaagtg gaaggatggt 1260
tctctccgaa ctgatattcc tgtccacgca ctcaacgtgt actttaacgt caacttcacc 1320
atcgtgtccc aggtcaaccc tcacatttct ctgttcatgt atgccccgcg gggaactgtg 1380
ggtaggccag tatctcaccg tcagggtaaa ggctggcgag gtgggttcct aggctcagct 1440
ttggaagaca tgctgaagct ggaaattcgt aaatggctca aactcatgaa aaaccttagt 1500
cttatgccac ggtttttcaa tcaagattgg tcttcagtat ggcttcaaac gttcgaggga 1560
tccgtcacct tgtggccaag gatcaggcta aaggactttt attatattct gtctgatccc 1620
actcgggaac aaatggaaac catgatcatt agtggacagc gatgcacatt cccaaagctc 1680
ttgttcatca agcaccaagt caacatagag cgggcaattg accgtggaag aaagcacaat 1740
gcaaaagcca gggaggaaaa tggtccccag cttagacggg taaacccatt cctgcacgac 1800
ttggatgacc gtgtatacca ttccagctct agcgtggacc ctcgcgagtt tcaggatgat 1860
cacgatgatg aagacgacga cagcactgat tctagcatgt aa 1902
<210> 89
<211> 662
<212> PRT
<213> 噬腺嘌呤阿克苏拉酵母
<400> 89
Met Gln Ser Leu Asp Leu Leu Asp Asp Arg Ser Trp Val Pro Asn Tyr
1 5 10 15
Ala Arg Val Gly Leu Lys Ser Leu Lys Glu Tyr Leu Val Ser His Arg
20 25 30
Tyr Gln Ser Glu Glu Ala Arg Lys His Ala Glu Ala Leu Glu Arg Trp
35 40 45
Thr Lys Ser Gln Ala Gln Ala Glu Thr Tyr Glu Gln Trp Leu Phe Ala
50 55 60
Ser Glu Gln Leu Asp Lys Leu Ser Gly Asn Asp Lys Trp Lys Glu Asp
65 70 75 80
Pro Val Ser Pro Tyr Tyr Asp Ser Val Leu Val Gln Gln Arg Leu Gln
85 90 95
Gln Leu Arg Asp Ala Arg Val Asn Ser Asn Met Asp Glu Leu Leu Tyr
100 105 110
Leu Val Arg Thr Ser Leu Gln Arg Asn Leu Gly Asn Met Gly Asp Pro
115 120 125
Arg Leu Tyr Val Arg Thr His Thr Gly Ser Lys Thr Leu Ile Glu Gln
130 135 140
Tyr Ile Ala Glu Val Glu Leu Ala Leu Asp Thr Leu Leu Ser Cys Gly
145 150 155 160
Pro Gly Thr Phe Ser Pro Lys Val Leu Leu Ser Asn Leu Ile Gln Thr
165 170 175
Arg Lys Ala Phe Gly Arg Thr Ala Leu Val Leu Ser Gly Gly Ser Thr
180 185 190
Phe Gly Ile Leu His Ile Gly Val Met Arg Glu Leu His Arg Ala His
195 200 205
Leu Leu Pro Gln Val Ile Ser Gly Ser Ser Ala Gly Ser Ile Phe Ala
210 215 220
Ser Met Leu Cys Ile His Leu Glu Asp Glu Ile Glu Glu Leu Leu Gln
225 230 235 240
Leu Pro Leu His Lys Glu Ser Phe Glu Ile Phe Glu Pro Ala Gly Glu
245 250 255
Arg Glu Gly Leu Met Val Arg Leu Ala Arg Phe Leu Lys His Gly Thr
260 265 270
Trp Phe Asp Asn Lys Tyr Leu Ser Thr Thr Met Arg Glu Leu Leu Gly
275 280 285
Asp Leu Thr Phe Gln Glu Ala Tyr Tyr Arg Thr Gln Arg Ile Leu Asn
290 295 300
Val Thr Val Ser Pro Ser Ser Met His Glu Met Pro Lys Ile Leu Asn
305 310 315 320
Tyr Leu Thr Ala Pro Asn Val Leu Ile Trp Ser Ala Val Cys Ala Ser
325 330 335
Cys Ser Val Pro Phe Val Phe Asp Ser His Asp Ile Leu Ala Lys Asn
340 345 350
Pro Arg Thr Gly Glu Phe Tyr Ser Trp Asn Ala Ser Thr Phe Ile Asp
355 360 365
Gly Ser Val Tyr Asn Asp Leu Pro Leu Ser Arg Leu Ala Glu Met Phe
370 375 380
Asn Val Asn His Phe Ile Ala Cys Gln Val Asn Pro His Val Val Pro
385 390 395 400
Phe Val Lys Phe Ala Glu Thr Met Ser Leu Val Glu Ala Arg Pro Thr
405 410 415
Thr Thr Glu Pro Gly Ser Leu Thr Lys Leu Trp His Ser Thr Gln Leu
420 425 430
Ala Leu Ser Ser Glu Ile Ser His Tyr Leu Asp Leu Ala Ala Glu Met
435 440 445
Gly Leu Phe Lys Asn Ile Ser Ser Lys Leu Arg Ser Val Leu Asp Gln
450 455 460
Gln Tyr Ser Gly Asp Ile Thr Ile Leu Pro Glu Leu Tyr Leu Ser Glu
465 470 475 480
Phe Gly Gln Ile Phe Lys Asn Pro Ser Lys Glu Phe Phe Gln Lys Ala
485 490 495
Glu Leu Arg Ala Ala Arg Ala Thr Trp Pro Lys Met Ser His Ile His
500 505 510
Asn Arg Val Ala Ile Glu Leu Ala Leu Val Lys Ala Ile His Lys Leu
515 520 525
Arg Ala Arg Ile Val Ser Gln Ser Val His Glu Pro Gly Ser Ser Leu
530 535 540
Gln Val His Ala Ala Asn Asp Glu Gly Thr Leu Ala Pro Ile Arg Arg
545 550 555 560
Arg His Ser Ser Thr Lys Leu His His Arg Arg Gln Arg Ser Asp Gly
565 570 575
Met Ala Val Lys Tyr Leu Val Arg Arg His Ser Leu Gln Tyr Phe Gly
580 585 590
Thr Glu Gly Pro Gly Pro Ala Ala Leu Ser Arg Lys Lys Ser Ser Ala
595 600 605
Gly Leu Thr Gln Ala His Thr Pro Thr Pro Ser Leu Thr Asn Ser Val
610 615 620
Ser Val Gly Gly Ser Pro Arg His Arg Arg Phe Thr Thr Ser Ser Arg
625 630 635 640
Gln Ser Ser Gly Asp His Leu Glu Met Phe Ser Gln Asn His Pro Leu
645 650 655
Glu Arg Ile Ser Thr Gly
660
<210> 90
<211> 1989
<212> DNA
<213> 噬腺嘌呤阿克苏拉酵母
<400> 90
atgcaatccc tggacctatt agacgacagg tcctgggtcc ccaattatgc gcgtgtgggc 60
ctgaaatcgc taaaagaata cttggttagc catagatatc agtctgaaga agctcgaaag 120
catgccgaag cgttagaaag atggacaaag tctcaggctc aggcggagac atacgaacag 180
tggctatttg cttcggagca gctcgacaag ctgtctggga acgacaagtg gaaagaggac 240
ccggtgtccc catattatga cagtgtgcta gtacaacagc ggttacagca gctccgagat 300
gctagggtga atagtaacat ggacgagctg ctgtatttgg tccgcactag cttgcaaaga 360
aacttgggta acatgggtga tcctcgacta tacgtgagga cccatactgg ctctaagacg 420
ctcattgaac aatatattgc tgaggtagaa ctggcattag acactctgct gagctgcgga 480
ccggggacgt tttcacccaa agttctgtta tccaatctta ttcagacaag aaaggcgttt 540
ggacgaacag ccctggtgct ttctggaggt agtacgtttg gaattttaca tattggtgta 600
atgcgagagc ttcaccgagc ccatctgtta ccgcaggtca tttctggatc gtcggccgga 660
tccatctttg cgtccatgct atgtattcac ttagaagacg agattgaaga actactgcaa 720
ctgcctctac acaaggaaag ctttgaaatc ttcgaacctg ctggagaacg agaaggacta 780
atggttcggc tggcacggtt cctcaaacat ggcacttggt tcgacaacaa gtatcttagc 840
acaactatgc gagagcttct aggagacctc actttccagg aggcctacta ccgaacgcag 900
cgaattctaa atgtcactgt gtctccttcg agtatgcacg aaatgccgaa gattctcaac 960
tatctgaccg ctcctaacgt gctcatttgg tcggcagtgt gtgcatcgtg ctcagtacca 1020
tttgtgtttg attctcacga cattctggca aaaaaccctc gaactgggga gttttattca 1080
tggaacgctt ctactttcat cgacgggagt gtgtataatg atctgccatt gtctcgacta 1140
gcggaaatgt ttaacgtgaa ccattttatt gcgtgccagg taaacccgca tgtggttcca 1200
ttcgtcaaat ttgccgagac aatgtcattg gtggaagctc gtcccactac tactgaaccg 1260
ggatcgttga caaagctatg gcacagtact cagctcgcgc tttctagtga gatctcacac 1320
tatctggatt tggctgctga aatgggcttg ttcaagaaca ttagttccaa gctgcgatcg 1380
gtgctagatc aacaatattc cggcgacatt actattcttc ccgaattata cctgtctgag 1440
tttggtcaga ttttcaaaaa cccatcaaag gagttcttcc agaaggcaga gcttcgagct 1500
gccagagcga catggcccaa gatgtcccac attcacaacc gtgtggccat cgagttggct 1560
ttagtaaagg caattcacaa gcttcgtgcc cgtattgtat ctcagagcgt ccatgagcct 1620
ggcagttctc tacaagtaca tgctgctaat gacgaaggca ccctagcacc tattcgccgt 1680
cgccattctt cgaccaagct tcaccataga cgacaacggt ccgatggaat ggccgtgaaa 1740
tacttggtcc gcagacattc gctacagtac tttggcactg agggccctgg tcccgctgcg 1800
ctatctcgta aaaagagttc ggccgggctt acccaggctc atactcctac gccttcactg 1860
accaacagcg ttagtgtagg gggcagtcca aggcaccgtc gcttcactac tagctctaga 1920
cagtcctcag gagaccattt ggaaatgttc tctcaaaatc atccgctaga acgtatctct 1980
accggctga 1989
<210> 91
<211> 666
<212> PRT
<213> 解脂耶氏酵母
<400> 91
Met Lys Ser Arg Val Ala Val Val Leu Ala Pro Val Leu Ala Pro Phe
1 5 10 15
Val Ala Ile Leu Lys Asn Leu Trp Val Phe Phe Thr Ala Leu Leu Glu
20 25 30
Leu Leu Phe Asp Val Ser Trp His Trp Met Leu Gln Ser Trp His Trp
35 40 45
Trp Cys Ser Thr Asp Gln Lys Thr Leu Leu Gln Leu Gln Leu Asp Gln
50 55 60
Ala Asp Thr Tyr Glu Glu Trp Glu Ser Ile Ala Ser Glu Leu Asp Glu
65 70 75 80
Leu Leu Gly Asn Asp Val Trp Arg Gln Thr Ala Ala Ser Lys Arg Tyr
85 90 95
Asp Tyr Arg Leu Ile Ala Gly Arg Leu Arg Asp Phe Ile Glu Cys Arg
100 105 110
Ala Val Gly Asp Ile Ala Thr Leu Ile Ser Arg Leu Arg Ser Gly Leu
115 120 125
Leu Arg Asn Leu Gly Ser Ile Ser Ser Leu Gln Leu Tyr Thr Arg Ser
130 135 140
Tyr Leu Gly Ser Lys Leu Leu Ile Glu Glu Tyr Ile Thr Glu Val Ile
145 150 155 160
Asp Cys Leu Lys Tyr Ile Lys Asp Tyr Gly Thr Thr Gly Gly Leu Asp
165 170 175
Thr Lys Gly Val His Phe Phe Pro Lys Ser Glu Gln Arg Gln Leu Asp
180 185 190
Ser Glu Gln Leu Thr Arg Gln Lys Lys His Lys Leu Phe Tyr Asp Thr
195 200 205
Arg Gln Ser Phe Gly Arg Thr Ala Leu Val Leu Gln Gly Gly Thr Ile
210 215 220
Phe Gly Leu Thr His Leu Gly Thr Ile Lys Ala Leu Thr Leu Gln Gly
225 230 235 240
Leu Leu Pro Gly Ile Val Thr Gly Phe Lys Glu Gly Ala Phe Ile Ala
245 250 255
Ala Leu Thr Gly Ile Tyr Val Ser Asp Leu Glu Leu Leu Glu Thr Ile
260 265 270
Asp Ser Leu Pro Asp Thr Leu Asn Asp Leu Tyr Gln Lys Tyr Lys Glu
275 280 285
Arg Leu Ala Glu Glu Asn Lys His Lys Asp His Ser Phe Ser Asn Ser
290 295 300
Asn Ser Asp Tyr Asp Phe Asp Tyr Ala Phe Asp Phe Glu Gln Phe Ala
305 310 315 320
Asn Thr Tyr Asn Val Thr Phe Ser Ser Val Thr Asp Lys Val Leu Arg
325 330 335
Ser Glu Tyr Pro Pro Glu Val Lys Met Tyr Glu Glu Phe Ile Glu Asn
340 345 350
Gln Leu Gly Asp Leu Thr Phe Glu Glu Ala Phe Asn Lys Ser Asp Arg
355 360 365
Val Leu Asn Ile Val Ala His Ser His Asp Ser Ser Phe Pro Thr Leu
370 375 380
Met Asn Tyr Leu Thr Thr Pro Asn Val Leu Ile Arg Ser Ala Cys Arg
385 390 395 400
Ala Ser Met Val Thr Ala His Asp Glu Pro Gln Thr Lys Lys Ala Cys
405 410 415
Ala His Leu Leu Val Lys Asp Asp Asp Asn Ser Val Ile Pro Tyr Asp
420 425 430
Ala Cys Lys Ser Arg Arg Gly Ser Ser Thr Asp Val Ile Leu Gly Pro
435 440 445
Val Gln Glu Glu Val Asp Pro Leu Asp Ser Thr Ala Asn Gly Thr Asn
450 455 460
Ser Ser Gly Pro Pro Lys Leu Glu Ile Thr Thr Asp Thr Trp Lys Arg
465 470 475 480
Asn Asn Ala Asp Asp Glu Asp His Val Asp Thr Leu Pro Gly Arg Val
485 490 495
Ser Ala Leu Pro Thr Pro Ser Tyr Ser Met Ile Asn Gln Gly Lys Ile
500 505 510
Val Ser Pro Tyr Ala Arg Leu Ser Glu Leu Phe Asn Val Asn His Phe
515 520 525
Ile Val Ser Leu Ser Arg Pro Tyr Leu Ala Pro Leu Leu Ala Ile Glu
530 535 540
Gly Arg His Arg Gly Tyr His Gly Trp Arg Val Asn Leu Ile Arg Val
545 550 555 560
Leu Lys Leu Glu Phe Glu His Arg Leu Ala Gln Phe Asp Tyr Ile Gly
565 570 575
Leu Leu Pro Thr Ile Phe Arg Arg Phe Phe Ile Asp Asp Lys Ile Pro
580 585 590
Gly Ile Gly Pro Asn Ala Glu Val Leu Ile Val Pro Glu Leu Ala Ala
595 600 605
Gly Met Ile Ser Asp Phe Lys Lys Ala Phe Ser Asn His Asp Ile Pro
610 615 620
Glu Lys Val Arg Tyr Trp Thr Thr Val Gly Glu Arg Ala Thr Trp Pro
625 630 635 640
Leu Val Ala Ala Ile Trp Ala Arg Thr Ala Ile Glu Tyr Thr Leu Asn
645 650 655
Asp Met Tyr Asn Gln Thr Lys Arg Gln Asn
660 665
<210> 92
<211> 2201
<212> DNA
<213> 解脂耶氏酵母
<400> 92
cttttacgag tgtgtatcat cacatgatta tgcagcaaga tcagtatcat ttcggctatc 60
cagctctctt cccccgttca gctccttttc taccgcgatt atgaaaagcc gcgtggccgt 120
tgtcttggcg ccggttctgg caccatttgt ggcgattttg aaaaacctgt gggtcttctt 180
cacagctcta ctggagctct tattcgacgt tagctggcac tggatgttac aatcatggca 240
ctggtggtgc tccaccgacc aaaaaacact gctacaactg cagctggacc aggcagacac 300
ctacgaggaa tgggaaagca ttgcatcgga gctggacgag ctgctgggca acgacgtgtg 360
gcgtcagacc gcagcctcga aacgatacga ctaccggctg attgcaggcc gtctgagaga 420
ctttatcgag tgccgggcgg tcggcgacat tgcgacgctg atttctcgtc tgcgaagcgg 480
actgctgcgg aatttgggct cgatttcgtc gctccagctg tacactcgct cgtacctcgg 540
ctctaaactg ctcatcgaag agtacatcac cgaggtcatt gactgtctca agtacatcaa 600
ggactatggg acgacgggcg gactggacac caagggagtg catttcttcc caaagtccga 660
acagcgacaa ctggacagtg aacagctgac tcgacaaaag aaacacaagt tattctacga 720
cacacgacaa tcttttggcc gaacggccct cgtgttgcag ggaggaacta ttttcggact 780
tactcatctc ggaacaatca aggctcttac tctccagggt ctgctaccgg gtattgtcac 840
cggtttcaag gagggggcgt ttattgccgc tctcacaggc atctacgtat ccgacctgga 900
gctgctcgaa accattgact ctttgccaga cactctcaat gacctgtacc aaaaatacaa 960
ggagcgactg gcggaggaaa acaaacacaa ggaccactcg ttcagtaact ccaattcgga 1020
ctacgacttt gactacgcat ttgactttga acagtttgca aacacctata atgtgacctt 1080
ctcgtctgtc actgacaaag tattgcgatc ggagtacccc ccggaagtca aaatgtacga 1140
ggagttcatc gagaatcaac tcggagacct cacgttcgaa gaggccttca acaaaagcga 1200
ccgcgtgctc aacattgtcg cccattccca tgactcttcc ttcccgacac tgatgaacta 1260
cctcaccact cccaatgtgc tcatcagaag cgcatgtaga gcttccatgg tgaccgccca 1320
cgacgagccc caaacgaaaa aggcatgtgc ccatctgctg gtcaaggatg acgacaacag 1380
cgtcattccc tatgacgcct gcaaatccag gcgaggaagc tcgaccgacg tgattctggg 1440
acctgtccag gaggaggtgg atccattaga ttcaacagct aacggtacta actcttctgg 1500
acctcccaaa ctcgaaatca caactgacac ctggaaacga aacaatgcag acgacgagga 1560
ccacgtggat actctcccgg gccgcgtgag tgctctacct acaccttcgt actccatgat 1620
taaccagggc aagattgtct ctccctacgc tcgcctttcc gaactcttta acgtcaacca 1680
cttcatcgtc tctctctcaa gaccctacct ggcgcctctt ctggccatcg aaggccgaca 1740
tagaggctac cacggctgga gagtgaacct gatccgagta ctgaaactag aattcgaaca 1800
cagactcgcc cagttcgact acataggcct gctgccgacc atcttccgtc ggttcttcat 1860
cgacgataag atccctggca tcggtcccaa cgccgaggtg ctcattgttc ctgagctagc 1920
ggctggcatg atctccgact tcaaaaaggc cttttcgaac cacgacattc ccgagaaggt 1980
ccgctactgg accactgtgg gcgaacgagc cacctggcct ctagtcgccg ccatctgggc 2040
cagaacagca atcgagtaca ccctcaacga catgtacaac cagaccaagc gacaaaacta 2100
gaccccgagc agagcacata actactaacg atgagactaa agtatgtact gtatgtacta 2160
aacatacgct cgtaaacagt tgtatttatt ctttttcagc a 2201
<210> 93
<211> 526
<212> PRT
<213> 解脂耶氏酵母
<400> 93
Met Glu Val Arg Arg Arg Lys Ile Asp Val Leu Lys Ala Gln Lys Asn
1 5 10 15
Gly Tyr Glu Ser Gly Pro Pro Ser Arg Gln Ser Ser Gln Pro Ser Ser
20 25 30
Arg Ala Ser Ser Arg Thr Arg Asn Lys His Ser Ser Ser Thr Leu Ser
35 40 45
Leu Ser Gly Leu Thr Met Lys Val Gln Lys Lys Pro Ala Gly Pro Pro
50 55 60
Ala Asn Ser Lys Thr Pro Phe Leu His Ile Lys Pro Val His Thr Cys
65 70 75 80
Cys Ser Thr Ser Met Leu Ser Arg Asp Tyr Asp Gly Ser Asn Pro Ser
85 90 95
Phe Lys Gly Phe Lys Asn Ile Gly Met Ile Ile Leu Ile Val Gly Asn
100 105 110
Leu Arg Leu Ala Phe Glu Asn Tyr Leu Lys Tyr Gly Ile Ser Asn Pro
115 120 125
Phe Phe Asp Pro Lys Ile Thr Pro Ser Glu Trp Gln Leu Ser Gly Leu
130 135 140
Leu Ile Val Val Ala Tyr Ala His Ile Leu Met Ala Tyr Ala Ile Glu
145 150 155 160
Ser Ala Ala Lys Leu Leu Phe Leu Ser Ser Lys His His Tyr Met Ala
165 170 175
Val Gly Leu Leu His Thr Met Asn Thr Leu Ser Ser Ile Ser Leu Leu
180 185 190
Ser Tyr Val Val Tyr Tyr Tyr Leu Pro Asn Pro Val Ala Gly Thr Ile
195 200 205
Val Glu Phe Val Ala Val Ile Leu Ser Leu Lys Leu Ala Ser Tyr Ala
210 215 220
Leu Thr Asn Ser Asp Leu Arg Lys Ala Ala Ile His Ala Gln Lys Leu
225 230 235 240
Asp Lys Thr Gln Asp Asp Asn Glu Lys Glu Ser Thr Ser Ser Ser Ser
245 250 255
Ser Ser Asp Asp Ala Glu Thr Leu Ala Asp Ile Asp Val Ile Pro Ala
260 265 270
Tyr Tyr Ala Gln Leu Pro Tyr Pro Gln Asn Val Thr Leu Ser Asn Leu
275 280 285
Leu Tyr Phe Trp Phe Ala Pro Thr Leu Val Tyr Gln Pro Val Tyr Pro
290 295 300
Lys Thr Glu Arg Ile Arg Pro Lys His Val Ile Arg Asn Leu Phe Glu
305 310 315 320
Leu Val Ser Leu Cys Met Leu Ile Gln Phe Leu Ile Phe Gln Tyr Ala
325 330 335
Tyr Pro Ile Met Gln Ser Cys Leu Ala Leu Phe Phe Gln Pro Lys Leu
340 345 350
Asp Tyr Ala Asn Ile Ser Glu Arg Leu Met Lys Leu Ala Ser Val Ser
355 360 365
Met Met Val Trp Leu Ile Gly Phe Tyr Ala Phe Phe Gln Asn Gly Leu
370 375 380
Asn Leu Ile Ala Glu Leu Thr Cys Phe Gly Asn Arg Thr Phe Tyr Gln
385 390 395 400
Gln Trp Trp Asn Ser Arg Ser Ile Gly Gln Tyr Trp Thr Leu Trp Asn
405 410 415
Lys Pro Val Asn Gln Tyr Phe Arg His His Val Tyr Val Pro Leu Leu
420 425 430
Ala Arg Gly Met Ser Arg Phe Asn Ala Ser Val Val Val Phe Phe Phe
435 440 445
Ser Ala Val Ile His Glu Leu Leu Val Gly Ile Pro Thr His Asn Ile
450 455 460
Ile Gly Ala Ala Phe Phe Gly Met Met Ser Gln Val Pro Leu Ile Met
465 470 475 480
Ala Thr Glu Asn Leu Gln His Ile Asn Ser Ser Leu Gly Pro Phe Leu
485 490 495
Gly Asn Cys Ala Phe Trp Phe Thr Phe Phe Leu Gly Gln Pro Thr Cys
500 505 510
Ala Phe Leu Tyr Tyr Leu Ala Tyr Asn Tyr Lys Gln Asn Gln
515 520 525
<210> 94
<211> 1581
<212> DNA
<213> 解脂耶氏酵母
<400> 94
atggaagtcc gacgacgaaa aatcgacgtg ctcaaggccc agaaaaacgg ctacgaatcg 60
ggcccaccat ctcgacaatc gtcgcagccc tcctcaagag catcgtccag aacccgcaac 120
aaacactcct cgtccaccct gtcgctcagc ggactgacca tgaaagtcca gaagaaacct 180
gcgggacccc cggcgaactc caaaacgcca ttcctacaca tcaagcccgt gcacacgtgc 240
tgctccacat caatgctttc gcgcgattat gacggctcca accccagctt caagggcttc 300
aaaaacatcg gcatgatcat tctcattgtg ggaaatctac ggctcgcatt cgaaaactac 360
ctcaaatacg gcatttccaa cccgttcttc gaccccaaaa ttactccttc cgagtggcag 420
ctctcaggct tgctcatagt cgtggcctac gcacatatcc tcatggccta cgctattgag 480
agcgctgcca agctgctgtt cctctctagc aaacaccact acatggccgt ggggcttctg 540
cataccatga acactttgtc gtccatctcg ttgctgtcct acgtcgtcta ctactacctg 600
cccaaccccg tggcaggcac aatagtcgag tttgtggccg ttattctgtc tctcaaactc 660
gcctcatacg ccctcactaa ctcggatctc cgaaaagccg caattcatgc ccagaagctc 720
gacaagacgc aagacgataa cgaaaaggaa tccacctcgt cttcctcttc ttcagatgac 780
gcagagactt tggcagacat tgacgtcatt cctgcatact acgcacagct gccctacccc 840
cagaatgtga cgctgtcgaa cctgctgtac ttctggtttg ctcccacact ggtctaccag 900
cccgtgtacc ccaagacgga gcgtattcga cccaagcacg tgatccgaaa cctgtttgag 960
ctcgtctctc tgtgcatgct tattcagttt ctcatcttcc agtacgccta ccccatcatg 1020
cagtcgtgtc tggctctgtt cttccagccc aagctcgatt atgccaacat ctccgagcgc 1080
ctcatgaagt tggcctccgt gtctatgatg gtctggctca ttggattcta cgctttcttc 1140
cagaacggtc tcaatcttat tgccgagctc acctgttttg gaaacagaac cttctaccag 1200
cagtggtgga attcccgctc cattggccag tactggactc tatggaacaa gccagtcaac 1260
cagtacttta gacaccacgt ctacgtgcct cttctcgctc ggggcatgtc gcggttcaat 1320
gcgtcggtgg tggttttctt tttctccgcc gtcatccatg aactgcttgt cggcatcccc 1380
actcacaaca tcatcggagc cgccttcttc ggcatgatgt cgcaggtgcc tctgatcatg 1440
gctactgaga accttcagca tattaactcc tctctgggcc ccttccttgg caactgtgca 1500
ttctggttca cctttttcct gggacaaccc acttgtgcat tcctttatta tctggcttac 1560
aactacaagc agaaccagta g 1581
<210> 95
<211> 346
<212> PRT
<213> 禾本红酵母
<400> 95
Met Gly Ala Gln Glu Glu Val Asp Tyr Asp Gln Ser Asp His Thr Lys
1 5 10 15
Ile Lys Phe Val Pro Phe Val Val Pro Arg His Arg Arg Leu Gln Thr
20 25 30
Phe Ser Val Phe Leu Trp Thr Thr Ala Leu Pro Ile Ser Leu Gly Ile
35 40 45
Phe Cys Ile Leu Cys Ser Phe Pro Pro Leu Trp Pro Leu Val Ile Gly
50 55 60
Tyr Leu Thr Trp Val Phe Leu Ile Asp Gln Ala Pro Met Arg Gly Gly
65 70 75 80
Arg Pro Gln Ala Trp Leu Arg Lys Ser Arg Val Trp Glu Trp Phe Ala
85 90 95
Gly Tyr Tyr Pro Val Ser Leu Ile Lys Ser Ala Asp Leu Pro Pro Asp
100 105 110
Gln Arg Tyr Val Phe Gly Tyr His Pro His Gly Val Ile Gly Met Gly
115 120 125
Ala Ile Ala Asn Phe Gly Thr Asp Ala Thr Gly Phe Ser Arg Leu Phe
130 135 140
Pro Gly Ile Thr Pro His Leu Leu Thr Leu Ala Ser Asn Phe Lys Leu
145 150 155 160
Pro Val Tyr Arg Glu Leu Leu Leu Ala Leu Gly Ile Ser Ser Val Ser
165 170 175
Met Lys Ser Cys Gln Asn Ile Leu Arg Gln Gly Pro Gly Ser Ser Ile
180 185 190
Thr Ile Val Val Gly Gly Ala Ala Glu Ser Leu Ser Ala His Pro Gly
195 200 205
Thr Ala Asp Leu Thr Leu Lys Arg Arg Lys Gly Phe Ile Lys Leu Ala
210 215 220
Ile Arg Thr Gly Ala Ser Leu Val Pro Val Phe Ser Phe Gly Glu Asn
225 230 235 240
Asp Ile Phe Asn Gln Leu Ser Asn Glu Arg Gly Thr Arg Leu Tyr Lys
245 250 255
Leu Gln Lys Arg Phe Gln Ala Val Phe Gly Phe Thr Leu Pro Ile Phe
260 265 270
Phe Gly Arg Gly Leu Phe Asn Tyr Asn Met Gly Leu Met Pro Tyr Arg
275 280 285
His Pro Ile Val Ser Val Val Gly Arg Pro Ile Lys Val Lys Gln Lys
290 295 300
Asp His Pro Ser Thr Ala Asp Leu Glu Glu Val Gln Glu Arg Tyr Ile
305 310 315 320
Ala Glu Leu Lys Arg Ile Trp Glu Asp Tyr Lys Glu Val Tyr Ala Lys
325 330 335
Ser Arg Thr Lys Glu Leu Thr Ile Ile Ala
340 345
<210> 96
<211> 1041
<212> DNA
<213> 禾本红酵母
<400> 96
atgggcgcac aagaagaggt cgactacgac cagtcggacc acaccaagat caagttcgtg 60
ccctttgtcg tcccgcggca ccgtcgcctc cagacgttct cggtcttcct gtggacgacg 120
gccctcccta tctcgctcgg catcttctgc atcctgtgct ccttccctcc tctttggccg 180
ctcgtcatcg ggtacctcac ctgggtcttc ctcattgacc aggcgccgat gcgcggcggg 240
aggccacaag cctggctgcg aaagtcgcgc gtgtgggagt ggttcgccgg ctactatccc 300
gtcagcctca tcaagagcgc cgacctcccg cccgaccagc gttacgtctt tggctaccac 360
cctcacggcg tcatcggcat gggcgccatc gccaactttg gcaccgacgc gaccgggttc 420
tcgcgcctgt tcccgggcat cacgccgcac ctcctcacgc tcgcgagcaa cttcaagctc 480
ccagtctacc gagagctcct cctcgccctc ggcatctcgt ccgtctcgat gaagagctgc 540
cagaacatcc tgcggcaagg tcccggctcg tccatcacga tcgtcgtcgg cggcgccgcc 600
gagagcctga gcgcgcaccc tggcacggcc gacctgacgc tcaagcgccg caagggcttc 660
atcaagctcg ccatccgcac cggcgcctcg ctcgtgcccg tcttttcctt tggcgagaac 720
gacatcttca accagctgtc gaacgagcga gggacgcgcc tgtacaagct gcagaagcgg 780
ttccaggccg tctttggctt cacattgccc atcttcttcg gccgaggcct gttcaactac 840
aacatgggct tgatgccgta ccgacacccg atcgtctcgg tcgtcggccg cccgatcaag 900
gtcaagcaga aggaccaccc gtcgactgcc gacctcgaag aagtccagga gcggtacatc 960
gccgagctca aaaggatctg ggaggactac aaggaggtgt acgccaagag tcgcaccaag 1020
gagctcacca tcatcgcctg a 1041
<210> 97
<211> 663
<212> PRT
<213> 禾本红酵母
<400> 97
Met Ser Thr Ala Asp Leu Pro Pro Gly Pro Ala Gln Leu Leu Glu Asp
1 5 10 15
Ala Leu Arg Gln Pro Asp Gly Pro Pro Leu Leu Ser Thr Ser Ala Ala
20 25 30
Asp Pro Ser Ser Pro Leu Gln Leu Asp His Asp His Arg Pro Gly Met
35 40 45
Ala Ala Asp Ala Ala Ser Ser Ala Ser Asp Ser Ser Ile Ser Thr Val
50 55 60
Ser Ser Val Leu Arg Gly Gln Gln Ala Thr Thr Thr Val Thr Thr Asn
65 70 75 80
Arg Gly Glu Gly Gly Arg Glu Thr Thr Glu Thr Phe Thr His Val Gly
85 90 95
Ala Ala Asn Val Asp Ala Glu Tyr Ser Ser Ser Thr Gly His Ile Thr
100 105 110
Leu Arg Pro Val Val Ala Lys Gly Gly Asp Pro Arg Arg Ile Arg Leu
115 120 125
Val Arg Ser Arg Arg Thr His Phe Glu Pro Arg Ile Ser His Phe Asp
130 135 140
Arg His Asn Lys Thr Ser Ala Glu Asp Thr Phe Arg Gly Phe Phe Ser
145 150 155 160
Leu Phe Trp Ile Val Ile Ala Val Gly Gly Thr Arg Thr Ile Tyr Asn
165 170 175
Arg Val Ala Glu Thr Gly Gly Leu Leu Gly Gly Trp Gln Phe Ala Ala
180 185 190
Leu Ile Ser Glu Asp Ala Trp Ala Leu Ala Leu Ser Asp Ala Val Leu
195 200 205
Val Gly Ser Thr Ile Leu Cys Val Pro Phe Val Lys Leu Ile Val Asn
210 215 220
Gly Trp Val Arg Tyr Tyr Tyr Thr Gly Leu Val Leu Gln His Leu Ala
225 230 235 240
Gln Thr Leu Tyr Leu Gly Ile Ala Val Arg Trp Thr Phe His Arg His
245 250 255
Trp Pro Trp Val Gln Ser Gly Phe Met Thr Leu His Ala Leu Ser Met
260 265 270
Leu Met Lys Ile His Ser Tyr Cys Ser Leu Asn Gly Glu Leu Ser Glu
275 280 285
Arg Val Arg Gln Leu Glu Lys Asp Glu Arg Lys Leu His Glu Ala Val
290 295 300
Glu Glu Leu Gly Gly Gln Asp Ala Leu Glu Arg Glu Gly Arg Val Ala
305 310 315 320
Trp Glu Lys Ala Cys Ala Glu Ala Ala Glu Gln Lys Ala Ala Glu Glu
325 330 335
Ala Ala Gly Gly Arg Gly Lys Ala Ser Ala Ser Ser Leu Ala Pro Pro
340 345 350
Pro Ala Thr Gly Pro Gln Pro Ser Ser Asp Glu Glu Ala Val Ser Thr
355 360 365
Thr Leu Arg Gln Arg Pro Ser Ala Ala Arg Arg Arg Ser Leu Ser Pro
370 375 380
Ser Ala Ala Arg Thr His Val Thr Pro Pro Ser Arg Lys Ala Glu Pro
385 390 395 400
His Asp Val Glu Thr Leu Thr Trp Ser Pro Asn Glu Arg Val Ser His
405 410 415
Leu Ala Ile Ala Ile Cys Glu Ala Arg Glu Ala Leu Ser Ser Ser Gly
420 425 430
Ala Ala Lys Val Ser Phe Pro Asp Asn Val Thr Val Leu Asn Phe Val
435 440 445
Asp Tyr Leu Leu Val Pro Thr Leu Val Tyr Glu Leu Glu Tyr Pro Arg
450 455 460
Thr Asp Ser Ile Arg Pro Leu Tyr Ile Leu Glu Lys Thr Leu Ala Thr
465 470 475 480
Phe Gly Thr Phe Ser Val Leu Leu Leu Ile Val Glu His Phe Ile Tyr
485 490 495
Pro Val Met Pro Gly Pro Asp Ser Ser Phe Ile Ser Ser Leu Leu Asp
500 505 510
Leu Ala Leu Pro Phe Thr Ile Cys Tyr Leu Leu Ile Phe Tyr Ile Ile
515 520 525
Phe Glu Cys Ile Cys Asn Ala Phe Ala Glu Ile Thr Arg Phe Ser Asp
530 535 540
Arg Ala Phe Tyr Ser Asp Trp Trp Asn Ser Ile Ser Phe Asp Glu Phe
545 550 555 560
Ser Arg Lys Trp Asn Arg Pro Val His Thr Phe Leu Leu Arg His Val
565 570 575
Tyr Ala Thr Thr Ile Ser Thr Tyr Lys Leu Ser Lys Phe Ser Ala Ala
580 585 590
Phe Val Thr Phe Leu Leu Ser Ala Leu Val His Glu Leu Val Met Val
595 600 605
Val Val Thr His Lys Ile Arg Met Tyr Leu Phe Met Ala Gln Leu Pro
610 615 620
Leu Ile Met Leu Gly Arg Ala Ser Ile Phe Lys Arg His Pro Ala Leu
625 630 635 640
Gly Asn Leu Phe Phe Trp Phe Gly Leu Leu Ser Gly Phe Pro Leu Leu
645 650 655
Ala Val Ala Tyr Leu Lys Phe
660
<210> 98
<211> 1992
<212> DNA
<213> 禾本红酵母
<400> 98
atgagcaccg ccgatcttcc accaggtcct gcccagctgc tcgaagacgc cctgcgccag 60
ccagacggcc cccctctcct gtcgacctcc gccgccgatc cctcctcccc acttcaactc 120
gaccacgacc accgccccgg catggctgca gacgccgcca gctcagcttc agacagctct 180
atcagcacgg tgtccagtgt cctgcgcggt cagcaagcca cgacaacggt gacgaccaac 240
aggggagaag gcgggcgaga aacgaccgag accttcaccc acgtcggcgc cgccaatgtc 300
gacgccgagt actcgtcctc gaccggccac atcacgctcc gacccgtcgt ggcaaagggc 360
ggtgaccctc gccggatccg cctcgtccgc tcgcgccgca cccacttcga gccgcgcatc 420
tcgcacttcg accgccacaa caagacgtcg gccgaggaca cgttccgcgg cttcttctcg 480
ctcttctgga tcgtcatcgc cgtcggcggc acgaggacca tctacaaccg cgtcgccgag 540
acgggcggtc tcctcggcgg gtggcagttt gcggcgctca tctccgagga cgcatgggct 600
ctggcgctga gcgatgcggt cctcgtcggg tcgacgatac tctgcgtccc gttcgtcaag 660
ctcatcgtca acggctgggt ccggtactac tacacgggcc tcgtcctcca gcacctcgcc 720
cagacgctct acctcggcat cgccgtccga tggacgttcc accgtcactg gccctgggtc 780
cagagcggct tcatgacgct gcacgccctg agcatgctca tgaagatcca ctcgtactgc 840
tcgctcaacg gcgagctgtc cgagcgcgtg cggcagctcg agaaggacga gcgcaagctg 900
cacgaggcgg tcgaggagct tggcggccag gacgcgctcg agcgcgaggg gcgcgtggcg 960
tgggagaagg cgtgcgccga ggcggccgag cagaaggcgg ccgaggaggc ggcaggcggt 1020
cgcggcaaag cttcggcgtc ctcgctcgcc ccgccgccgg cgacagggcc gcagccctcg 1080
tccgacgagg aggccgtctc gacgacgctc cgacagcgac cgtcggccgc tcgccgccgc 1140
tcgctctcgc cgtcggccgc acggacccac gtcacgccgc cgtcgcgcaa ggccgagccg 1200
cacgacgtcg agacgctcac ctggtcgccc aacgagcgcg tgtcgcacct cgccatcgcc 1260
atctgcgagg cacgcgaggc cctgtcgtcg agcggcgccg ccaaggtctc gttcccggac 1320
aacgtcacgg tcctcaactt tgtcgactac cttctcgtcc cgacgctcgt gtacgagctt 1380
gagtacccga ggaccgactc tatccgaccc ttgtacatcc tcgagaagac cctcgccacg 1440
ttcggcacat tctcggtcct cctcctcatc gtcgagcact tcatctaccc ggtcatgccc 1500
gggcccgaca gctcgttcat ctcgtccctc ctcgacctcg ccctcccatt caccatctgc 1560
tacctcctca tcttctacat catcttcgag tgtatctgca acgccttcgc cgagatcacg 1620
cgcttctcgg accgggcctt ctacagcgac tggtggaact cgatctcgtt cgacgagttc 1680
tcgcgcaagt ggaaccggcc cgtgcacacg ttcctcctgc gccacgtgta cgcgacgacc 1740
atctcgacct acaagctcag caagttctcg gccgcctttg tcacgttcct cctgagcgcg 1800
ctcgtgcacg agctcgtcat ggtagtcgtg acgcacaaga tccgcatgta tctctttatg 1860
gcgcagctcc ccctcatcat gctcggccga gcaagcatct tcaagcgtca ccctgcgctc 1920
ggcaacctct tcttctggtt cggcctcttg agcggtttcc ctctgctagc tgtagcgtac 1980
ctcaagttct ag 1992
<210> 99
<211> 635
<212> PRT
<213> 季也蒙毕赤酵母
<400> 99
Met Thr Lys Glu Val Asp Glu Ser Thr Gly Gly Ala Ser Asp Ile Pro
1 5 10 15
Asn Met Val Glu Glu Ala Lys Ser Ser Ser Phe Asp Arg Glu Thr Glu
20 25 30
Glu Asn Leu Leu Leu Glu Thr Thr Lys Pro Asp Glu Asn Leu Val Pro
35 40 45
Glu Ser Thr Lys His Asp Glu Lys Leu Val Pro Glu Ile Thr Lys His
50 55 60
Glu Asp Asn Pro Met Glu Asn Asp Gln Val Ser Gln Asn Thr Ala Thr
65 70 75 80
Ser Pro Met Thr Gly Ala Gly Ser Glu Glu Thr Arg Asp Leu Ile Thr
85 90 95
Glu Asn Ile Glu Lys Pro Asp Glu Gly Asp Leu Leu Ile Glu Leu Ile
100 105 110
Ser Lys Asp Asn Asp Gly Asp Gly Asp Asp Gly Leu Lys Asn Arg Lys
115 120 125
Gln Lys Arg Ser Ser Ser Glu Val Lys Arg Leu Arg Met Ser Ser Leu
130 135 140
Ala Pro Lys Gly Pro Thr Pro Gln Lys His Glu Arg Pro Lys Tyr Ile
145 150 155 160
Asn Val Ala Pro Leu Asn Ile Pro Ile Arg Arg Arg Leu Glu Met Val
165 170 175
Gly Ile Ile Trp His Thr Ile Cys Ile Pro Thr Phe Val Ser Leu Phe
180 185 190
Phe Leu Thr Leu Ser Leu Gly Pro Phe Ala Trp Val Gly Val Ile Leu
195 200 205
Pro Tyr Phe Leu Trp Trp Tyr Leu Ile Asp Leu His Thr Pro Thr Asn
210 215 220
Gly Lys Val Ala Tyr Arg Ser Arg Asp Trp Met Lys Asn Phe Ile Val
225 230 235 240
Trp Asp Trp Phe Val Asp Tyr Phe Pro Ile Arg Val His Lys Ser Cys
245 250 255
Glu Leu Glu Pro Thr Phe Ser Asp Val Ile Ile Glu Asp Asp Val Val
260 265 270
Pro Asp Asp Glu Glu Asp Leu Ile Ser Glu Gln Ser Arg Thr Gly Val
275 280 285
Asp Lys Leu Phe Lys Phe Leu Gly Leu Arg Lys Arg Leu Asn Asp Asp
290 295 300
Ser Asp Ala Ser Ser Gln Cys Ser Leu Leu Gln Glu Ser Leu Ser Thr
305 310 315 320
Arg Arg Lys Val Lys Arg Met Ser Thr Gly Pro Arg Tyr Ile Phe Gly
325 330 335
Tyr His Pro His Gly Val Ile Ser Met Gly Val Phe Gly Thr Phe Ala
340 345 350
Thr Asn Ala Leu Arg Asn Glu Pro Tyr Glu Pro Pro Leu Arg Leu Leu
355 360 365
Lys Pro Phe Phe His Asp Ser Ser Lys Gly Glu Arg Leu Phe Pro Gly
370 375 380
Ile Gly Thr Val Phe Pro Leu Thr Leu Thr Thr Gln Phe Ile Val Pro
385 390 395 400
Tyr Tyr Arg Asp Tyr Ile Leu Gly Met Gly Leu Thr Ser Ala Ser Ala
405 410 415
Lys Asn Ile Lys Ser Leu Ile Ser Asn Gly Asp Asn Ser Ile Cys Val
420 425 430
Val Val Gly Gly Ala Gln Glu Ser Leu Leu Asn Asp Met Val Ala Ala
435 440 445
Thr Thr Val Pro Gly Arg Tyr Gly Lys Ser Asn Leu Pro Asn Asp Ser
450 455 460
Asp Thr Asp Ser Glu Phe Asp Pro Gln Arg Lys Ile Glu Glu Asn Lys
465 470 475 480
Glu Glu Thr Gly Val Lys Lys Ile Glu Leu Val Leu Asn Lys Arg Lys
485 490 495
Gly Phe Val Lys Ile Ala Ile Glu Leu Gly Asn Val Ser Leu Val Pro
500 505 510
Thr Phe Gly Phe Gly Glu Ala Asp Ile Tyr Arg Ile Thr Lys Pro Lys
515 520 525
Pro Gly Ser Phe Gly Glu Met Phe Gln Ser Trp Met Lys Arg Thr Phe
530 535 540
Gln Phe Thr Val Pro Phe Phe Ser Ala Arg Gly Val Phe Ile Tyr Asp
545 550 555 560
Phe Gly Phe Leu Pro Tyr Arg Asn Pro Ile Asn Val Cys Phe Gly Arg
565 570 575
Pro Ile His Ile Pro Ala Gly Leu Leu Asp Gln Tyr Lys Glu Pro Glu
580 585 590
Thr Glu Lys Asp Glu Lys Glu Lys Glu Lys Asn Val Phe Gln Phe Thr
595 600 605
Gln Asp Lys Gln Ala Pro Ala Phe Asn Ile Gln Ser Ile Gln Val Phe
610 615 620
Gln Gly Glu Ala Thr Ile Lys Glu Glu Thr Ser
625 630 635
<210> 100
<211> 1908
<212> DNA
<213> 季也蒙毕赤酵母
<400> 100
atgaccaagg aggttgatga aagcactggg ggtgccagtg atataccaaa tatggttgaa 60
gaagcgaaat catcgagttt tgaccgtgaa actgaagaga atctgctact ggagaccact 120
aaacctgacg agaatctggt accggagagt actaaacatg acgagaaact tgtaccggag 180
atcactaaac atgaagacaa tcccatggaa aatgaccaag tttcccaaaa cacagccacc 240
agtcctatga caggagctgg ttccgaagaa acccgtgatt tgattacaga gaacattgag 300
aaaccagatg agggtgatct gctaattgag cttatttcca aagataacga tggtgatgga 360
gatgatgggt tgaaaaatag aaaacaaaaa cgatcttctt ctgaagtgaa aaggctgcgc 420
atgtcgtctc tggctcctaa aggtccaact cctcaaaagc atgaacgtcc caagtatata 480
aatgtggcac ctcttaatat ccccattcga cggcgcttgg agatggtggg gataatctgg 540
cacaccattt gtattcccac gtttgtcagt ttgtttttct tgactttgtc gttgggtccg 600
tttgcttggg taggggtgat attgccgtac tttttatggt ggtatcttat cgatttacat 660
actcctacaa acggtaaggt tgcgtatcgg tctcgcgact ggatgaagaa tttcattgtg 720
tgggattggt tcgttgacta ttttcctatc agggtccaca agtcttgtga gttggagcct 780
acctttagcg atgttattat tgaagacgat gtggtgcccg atgatgaaga agaccttatc 840
tcagagcaat cacgaactgg agtcgataaa cttttcaaat ttttggggct tcgaaaacgc 900
ttaaatgacg actcggatgc ttcgtcgcag tgctcactgc tgcaagagtc tttaagcaca 960
agacgtaaag tgaaacgtat gtctactggt cctcgctaca tctttggata ccatccccat 1020
ggagtaattt cgatgggtgt ttttggaact ttcgctacca atgcgttgcg taacgagccg 1080
tacgaacctc ccttgcgttt gctaaagcca tttttccacg actcttccaa gggagaacgg 1140
ttgtttcccg gtattggcac cgtctttcca ttgacattga caacccaatt tattgtgccg 1200
tactaccgtg actatatctt gggcatggga ctcaccagtg cttcggctaa aaacatcaag 1260
agccttataa gcaacggaga caactcgata tgtgtcgttg ttggaggtgc tcaggaatcg 1320
ctcctaaacg atatggtagc cgcaaccaca gttcccggtc gttacggaaa gagcaatttg 1380
cccaatgaca gtgataccga tagcgagttt gatcctcagc gtaagattga agaaaacaag 1440
gaagaaaccg gcgtaaagaa aattgaactt gtacttaata agagaaaggg tttcgtcaag 1500
atagcgattg agttgggcaa cgtttcactc gtgcctacgt ttggttttgg agaagctgac 1560
atctacagaa tcaccaaacc caaaccaggt tcatttggag aaatgttcca atcttggatg 1620
aaacgcacat ttcaattcac ggttccattt ttcagcgcta gaggtgtgtt catttacgac 1680
tttgggtttc ttccttacag aaatcccatc aatgtctgct ttggacggcc cattcatatt 1740
ccagccggct tattggatca atacaaagag cccgaaactg agaaagatga aaaagaaaag 1800
gaaaaaaacg tcttccagtt cactcaagac aaacaagcgc cagccttcaa tatccaatct 1860
attcaagttt tccaagggga agcaaccatc aaagaggaaa cgagttag 1908
<210> 101
<211> 597
<212> PRT
<213> 季也蒙毕赤酵母
<400> 101
Met Ser Lys Glu Asn Leu Leu Lys Ile Ser Gln Tyr Asn Thr Glu Arg
1 5 10 15
Arg Pro Ser Leu Ala Thr Asp Val Asp Tyr Ser Ser Thr Asp Leu Ser
20 25 30
Ser Arg Leu Asp Ser Ala Asn Thr Thr Asn Gly Thr Pro Thr Val Thr
35 40 45
Leu His Lys Arg Gln Ser Ser Thr Glu Leu Leu Ser Glu Ser Pro Glu
50 55 60
Gln Lys Arg Phe Leu Lys Thr Ile Asp Thr Leu Asn Arg Thr Thr Asn
65 70 75 80
Ser Arg Leu Arg Gln Arg Leu Asn Arg Glu Gly Asp Lys His Lys Lys
85 90 95
Glu His Lys Glu His Glu Lys His Lys Asp Asp His Ser Lys Tyr Lys
100 105 110
Ser Arg Phe Gly Asp Ile His Phe Tyr Ser Asn Met Thr Thr Ile Phe
115 120 125
Asp Ala Asp Tyr Phe Lys Glu Ser Gln Phe Phe Gly Val Tyr Ile Leu
130 135 140
Phe Trp Leu Gly Thr Ala Phe Leu Ile Leu Asn Asn Leu Val His Thr
145 150 155 160
Phe Leu Glu Asn Gly Asp Asn Leu Leu Asp Gly Pro Val Val Arg Thr
165 170 175
Phe Lys Lys Asp Leu Leu Lys Ile Ala Leu Thr Asp Leu Gly Met Tyr
180 185 190
Leu Thr Met Tyr Val Ser Val Phe Ile Gln Leu Gly Ile Arg Lys Gly
195 200 205
Trp Tyr Ser Trp Ser Ser Thr Gly Ala Thr Leu Gln Asn Ile Tyr Ser
210 215 220
Phe Val Tyr Phe Phe Ala Trp Ser Tyr Phe Ala Ser Pro Lys Tyr Met
225 230 235 240
Asp Tyr Pro Trp Ile Gly Lys Val Phe Leu Ala Leu His Ser Leu Val
245 250 255
Phe Leu Met Lys Met His Ser Tyr Ala Thr Tyr Asn Gly Tyr Leu Trp
260 265 270
Asn Ile Phe Asn Glu Leu Gln Val Ser Arg Lys Tyr Leu Lys Ile Leu
275 280 285
Asp Glu Thr Asp Glu Ser Met Ile Glu Gly Lys Ser Val Ser Asp Leu
290 295 300
Arg Lys Ala Leu Val Asp Ser Ile Gly Phe Cys Ser Tyr Glu Leu Glu
305 310 315 320
Tyr Gln Ser Lys Ser Thr Ser Val Asn Thr Asp Val Glu Ile Thr Gly
325 330 335
Asp Lys Asn Lys Leu Asn Thr Thr Lys Ser Thr Ser Ser Leu Asp Asp
340 345 350
Asp Tyr Val Ser Phe Pro Asn Asn Ile Thr Phe Phe Asp Phe Phe Arg
355 360 365
Tyr Ser Met Phe Pro Thr Val Val Tyr Ser Leu Lys Phe Pro Arg Thr
370 375 380
Lys Arg Ile Arg Trp Gly Tyr Val Met Glu Lys Ser Phe Ala Val Phe
385 390 395 400
Gly Ile Ile Phe Leu Met Ile Thr Val Ala Gln Asn Trp Met Tyr Pro
405 410 415
Ile Val Val Arg Ala Gln Glu Ala Ser Lys Leu Pro Met Ser Arg Glu
420 425 430
Lys Val Leu Gln Tyr Cys Leu Val Leu Leu Asp Met Ile Pro Pro Phe
435 440 445
Leu Met Glu Tyr Leu Phe Thr Phe Phe Leu Ile Trp Asp Val Ile Leu
450 455 460
Asn Ala Ile Ala Glu Leu Ser Arg Phe Ala Asp Arg Asp Phe Tyr Gly
465 470 475 480
Pro Trp Trp Ser Cys Thr Asp Trp Ser Glu Phe Ala Arg Ile Trp Asn
485 490 495
Arg Pro Val His Lys Phe Leu Leu Arg His Val Tyr Gln Ser Thr Ile
500 505 510
Ser Thr Phe Lys Leu Asn Lys Asn Gln Ala Ser Leu Val Thr Phe Ile
515 520 525
Ile Leu Ser Phe Val His Glu Phe Val Met Phe Val Ile Phe Arg Lys
530 535 540
Val Arg Phe Tyr Met Leu Ala Leu Gln Met Ser Gln Leu Pro Leu Ile
545 550 555 560
Met Ile Ser Arg Thr Lys Phe Met Arg Asp Lys Lys Val Leu Gly Asn
565 570 575
Val Ile Cys Trp Val Gly Phe Ile Ser Gly Pro Ser Met Ile Cys Thr
580 585 590
Leu Tyr Leu Val Phe
595
<210> 102
<211> 1794
<212> DNA
<213> 季也蒙毕赤酵母
<400> 102
atgtccaagg aaaacttact taagatcagc cagtataata ctgagagaag accgtcgttg 60
gccacagacg ttgactactc ttccaccgat ttatccagtc gtctggattc ggccaacacg 120
acaaacggaa caccgaccgt aactcttcac aagaggcaat cgtctacaga gctcttgtct 180
gagtcacctg aacagaaaag gttcttgaaa acgatagaca ctttgaatcg aaccacaaat 240
tctagattac gccagaggtt aaaccgtgag ggcgataagc ataaaaagga acacaaagaa 300
catgaaaaac ataaagatga ccattctaaa tacaagtctc ggtttggaga tatccatttc 360
tactcaaaca tgacaaccat cttcgatgct gattacttta aggaatcgca gttctttgga 420
gtttacattc tcttttggct cggaacggca ttcttaattc tcaacaactt ggtccataca 480
tttttggaga acggagacaa tcttctcgat ggaccagttg tcagaacgtt taaaaaggac 540
ttacttaaaa ttgctcttac agacttggga atgtacttga cgatgtacgt ctctgtcttt 600
attcaattgg gcatccgcaa aggatggtat agctggagct caacaggagc caccttgcaa 660
aacatatact cattcgtgta cttctttgcc tggagttact ttgcgtcgcc aaagtacatg 720
gactaccctt ggattggaaa ggtgtttctt gcacttcaca gcttggtgtt tctcatgaaa 780
atgcattctt atgccacata caacggctat ctttggaaca tcttcaacga gcttcaagtg 840
tcacgaaagt acttgaagat attggacgag accgatgaat ccatgattga gggtaagagt 900
gtttccgatt tgcgaaaggc tttggtagac agcattggtt tctgctcata cgagttggag 960
taccagtcca aatcaacgag cgtgaacacg gatgtcgaaa tcaccggcga caagaacaaa 1020
ttgaacacaa ccaagtctac cagttcactc gatgacgact atgtgagttt ccccaataac 1080
attacgtttt tcgatttttt caggtattca atgtttccaa cagtggtgta ttctctcaag 1140
ttcccacgta caaagcgtat tagatggggt tacgtcatgg aaaagtcatt tgcagtgttt 1200
ggcatcatct tcttgatgat caccgtcgct caaaactgga tgtatcctat cgttgtacga 1260
gcacaagagg ctagcaaact cccaatgtca agagaaaagg tattgcagta ctgtttggtt 1320
ttactagaca tgattccacc atttctcatg gaatatcttt tcaccttttt cttgatttgg 1380
gacgtgatcc taaatgcgat agccgaattg agtaggtttg cagatcggga cttttatggt 1440
ccttggtggt cttgtaccga ttggtcggaa tttgcaagaa tttggaatcg tcctgttcac 1500
aaatttttgc ttcgtcatgt gtaccagtca actatcagta ctttcaaact caataaaaac 1560
caagcgtcgt tggtgacgtt tatcattctg agttttgttc atgagtttgt catgtttgtc 1620
atttttagaa aggtgagatt ctacatgttg gcgctccaga tgtctcagct tccattgata 1680
atgattagtc gaacaaaatt catgagagac aaaaaagtgt tgggaaatgt tatctgctgg 1740
gtaggattca tttctggacc atcgatgatc tgtactttgt atttagtatt ttaa 1794
<210> 103
<211> 515
<212> PRT
<213> 噬腺嘌呤阿克苏拉酵母
<400> 103
Met Ala Thr Ala Thr Ala Ile Ala Thr Val Thr Glu Gly Leu Gly Leu
1 5 10 15
Asp Lys Val Leu Ser Lys Glu Gln Pro Gly Leu Ser Lys Leu Ala Pro
20 25 30
Arg Ala Asn Thr Asn Val Gln Pro Thr Gln Leu Gln Ser Pro Ser Pro
35 40 45
Pro Gln Ser Arg Ser Ser Ser Pro Ile Ser Ala Ser Ser Ser Ser Glu
50 55 60
Ser Leu Glu Leu Lys Val Pro Lys Ala Lys Ser Pro Ser Ser Ser Lys
65 70 75 80
His Lys Pro His Tyr Arg Pro Val His Val Arg Ser Thr Ala Ser Ile
85 90 95
Leu Ser Arg Asp Pro Ala Ala Arg Thr Glu Pro Pro Ser Tyr Ser Gly
100 105 110
Phe Arg Asn Leu Ala Met Ile Ala Leu Ala Val Ser Asn Met Arg Leu
115 120 125
Leu Leu Glu Asp Tyr Gln Asn Tyr Gly Val Phe His Thr Leu Asn Ile
130 135 140
Met Gly Leu Ser Ala His Asp Val Arg Leu Thr Leu Ala Leu Thr Ala
145 150 155 160
Ser Val Pro Phe His Leu Phe Val Ala Leu Ala Ile Glu Arg Ile Ala
165 170 175
Val Leu Thr Met Pro Ser Lys Ser Thr Ala His Asn His Arg Ser Lys
180 185 190
His Leu Trp Gly Leu Phe Ala Val Leu His Ala Leu Asn Ala Ala Ala
195 200 205
Val Leu Ala Ile Ser Ser Tyr Thr Val Tyr Ser Arg Met Trp Ser Pro
210 215 220
Ala Val Gly Thr Leu Cys Glu Cys His Ala Ile Val Val Cys Phe Lys
225 230 235 240
Val Ala Ser Tyr Ala Leu Thr Asn Arg Asp Leu Arg Asp Ala Ala Ile
245 250 255
Asp Gly Leu Glu Thr Thr Asp Pro Leu Leu Ser Lys Leu Pro Tyr Pro
260 265 270
Ser Asn Leu Thr Leu Ser Asn Leu Val Tyr Phe Trp Trp Ala Pro Thr
275 280 285
Leu Val Tyr Gln Pro Ile Tyr Pro Arg Trp Pro Leu His Arg Arg Trp
290 295 300
Gly Phe Ile Phe Ser Arg Leu Leu Glu Ile Met Gly Ser Met Val Leu
305 310 315 320
Ile Trp Phe Ile Ser Thr Gln Tyr Ala Asn Pro Ile Leu Glu Ser Ser
325 330 335
Leu Gly His Phe Glu Gln Phe Asn Val Val Lys Ile Ser Glu Cys Leu
340 345 350
Leu Lys Leu Ala Ser Val Ser Met Ala Ile Trp Leu Leu Gly Phe Phe
355 360 365
Cys Leu Phe Gln Ser Phe Leu Asn Leu Leu Ala Glu Leu Val Arg Phe
370 375 380
Gly Asp Arg Glu Phe Tyr Gln Asp Trp Trp Asn Ala Gly Ser Val Gly
385 390 395 400
Thr Tyr Trp Arg Lys Trp Asn Arg Pro Val His Asn Tyr Phe Leu Arg
405 410 415
His Phe Tyr Ile Pro Met Leu Lys Arg Gly Tyr Ser Gln Arg Thr Ala
420 425 430
Ser Val Ile Val Phe Phe Leu Ser Ala Ile Leu His Glu Val Ala Val
435 440 445
Gly Val Pro Thr Gln Ser Leu Ile Gly Val Ala Phe Val Gly Met Gly
450 455 460
Ala Gln Ile Pro Leu Val Leu Ala Thr Ser Pro Leu Glu Lys Met Gly
465 470 475 480
Glu Thr Gly Ala Thr Ile Gly Asn Cys Ile Phe Trp Leu Ser Phe Phe
485 490 495
Leu Gly Gln Pro Met Gly Val Leu Leu Tyr Tyr Phe Ala Trp Asn Met
500 505 510
Lys His Gln
515
<210> 104
<211> 1548
<212> DNA
<213> 噬腺嘌呤阿克苏拉酵母
<400> 104
atggccaccg ctactgctat cgctacggtc acggagggcc tgggactaga taaggtgcta 60
tccaaggagc agccaggctt gtcgaagcta gctcctcgag cgaatacaaa tgtacaaccg 120
acccagttgc agtccccgtc tccaccacaa tctcgatctt cgtctccaat ttcggcctcc 180
tcatcatcag agtccctgga gctcaaggtg cccaaggcca aatcgccatc atcttccaaa 240
cacaaaccac actaccgccc cgtgcatgtg cggtcaacag catccatcct gtccagagac 300
ccggccgcca gaaccgagcc tccctcttac tctgggttca ggaacctagc catgattgca 360
ttggcggttt ctaatatgcg cctccttctc gaggactatc aaaactatgg cgtgttccac 420
actctcaaca ttatgggctt gagcgcacac gacgttcgcc tcacactggc attgacagct 480
tcggttccgt tccatctgtt tgtggccctg gccattgagc gcatcgcagt cctcactatg 540
ccctccaaat ctacagcaca caaccaccgc tcaaagcatc tctggggctt gtttgcagtt 600
ctgcatgctc tcaacgccgc tgctgtgcta gcaatcagct catacaccgt atacagtcgc 660
atgtggagtc ctgctgtggg aacattgtgc gaatgccacg caatcgtggt atgctttaag 720
gtggcatcgt atgcgcttac caaccgagac ttacgagatg ctgccattga tgggctagag 780
acaactgacc ctctgttgtc caagttgccc tacccatcca accttacctt gtcaaatctc 840
gtgtatttct ggtgggcccc aaccctagtg tatcagccaa tttaccctcg atggcccctg 900
catcgacgat ggggcttcat cttttctcgc ctgctcgaga ttatgggatc tatggtacta 960
atctggttca tttccaccca atacgccaac cccattttgg aatcatcctt ggggcacttt 1020
gaacagttta acgtggttaa aatctcagaa tgtctcctca aattagcatc ggtctccatg 1080
gccatctggc ttttgggttt cttttgtctc tttcaatcgt ttttgaactt gctggcagaa 1140
ttggttcgtt ttggcgaccg cgagttctac caagactggt ggaacgccgg ctcagtaggt 1200
acctactggc gcaaatggaa ccgaccagtg cacaactatt tcttgcgcca tttctacatc 1260
ccaatgctca agcgaggtta ttcacagcgc actgcctcgg tcattgtatt ctttttatct 1320
gccattctcc atgaagttgc tgttggcgtg cctactcagt ccttgattgg agttgcgttt 1380
gtaggcatgg gtgcccagat tcctctagtg ctggccacta gtcctttgga aaagatgggc 1440
gaaactggcg caactattgg caactgcatc ttttggctct ctttcttcct gggccagcca 1500
atgggggtac tgctttacta ctttgcgtgg aatatgaagc accagtag 1548
<210> 105
<211> 687
<212> PRT
<213> 噬腺嘌呤阿克苏拉酵母
<400> 105
Met Ala Val Lys Arg Arg Ser Asn Leu Pro Gln Gln Glu Val Asp Ser
1 5 10 15
Asp Ser Ser Ser Asp Ser Ser Ser Val Ser Ser Ser Ala Ala Ser Ser
20 25 30
Ser Val Asp Leu Ala Thr Gly Ser Ser Ser Gln Ile Glu Asp Arg Phe
35 40 45
Pro Lys Lys Thr Ala Ser Ala Thr Lys Arg Ser Ser His His Arg Lys
50 55 60
Ser Lys Lys Asp Gly Gly His Leu Ser Tyr Lys Trp Arg Leu Ala Tyr
65 70 75 80
Ser Lys Arg Leu Val Phe Ile Ile Gly Ile Ile Phe Gly Leu Gly Ile
85 90 95
Ala Trp Tyr Ser Ala Pro Lys Glu Phe Val Ser Leu Asp Arg Leu Ser
100 105 110
Glu Leu Ser Leu Asp Gly Leu Leu Asp Glu Phe Arg Asp Met Leu Pro
115 120 125
Lys Gly Ile Met Arg Glu Ala His Asp Ile Asp Lys Lys Ser Tyr Thr
130 135 140
Leu Ser Asp Ser Phe Ala Val Gly Asn Tyr Leu Arg Glu Glu Gly Tyr
145 150 155 160
Gly Val Lys His Pro Val Ile Leu Ile Pro Gly Val Ile Ser Thr Gly
165 170 175
Leu Glu Ser Trp Gly Leu Glu Gly Thr Glu Glu Cys Pro Ser Gln Pro
180 185 190
His Phe Arg Lys Arg Leu Trp Gly Ser Leu Tyr Met Leu Arg Thr Met
195 200 205
Leu Leu Asp Lys His Cys Trp Leu Lys His Ile Met Leu Asp Pro Ser
210 215 220
Thr Gly Leu Asp Pro Pro Gly Tyr Lys Ile Arg Ala Ala Leu Gly Met
225 230 235 240
Glu Ser Ala Asp Phe Phe Val Pro Gly Tyr Trp Leu Trp Asn Lys Ile
245 250 255
Leu Glu Asn Leu Ala Ala Met Gly Tyr Asp Ser Asn Asn Met Leu Val
260 265 270
Ala Ser Tyr Asp Trp Arg Leu Ser Tyr Pro Asp Leu Glu Arg Arg Asp
275 280 285
Ser Tyr Phe Ser Arg Leu Lys Ser Ala Ile Glu His Ser Val His Ser
290 295 300
Thr Gly Glu Lys Val Ala Leu Val Gly His Ser Met Gly Thr Gln Val
305 310 315 320
Ile Phe Tyr Phe Leu Lys Trp Ala Glu Ala Lys Gly Tyr Gly Asp Gly
325 330 335
Gly Asp Gln Trp Val Asn Asp His Ile Ala Ser Leu Val Asp Ile Ser
340 345 350
Gly Ser Thr Leu Gly Thr Pro Lys Ala Ile Val Ala Leu Leu Ser Gly
355 360 365
Glu Met Lys Asp Thr Val Gln Leu Asn Ala Leu Ala Val Tyr Gly Leu
370 375 380
Glu Lys Phe Phe Ser Arg Arg Glu Arg Ala Asp Met Leu Arg Ser Phe
385 390 395 400
Gly Gly Ile Ala Ser Met Leu Pro Lys Gly Gly Glu Ala Val Trp Gly
405 410 415
Asn Leu Thr Phe Ala Pro Asp Asp Pro Pro Ile Thr Pro Ala Asp Ala
420 425 430
Gln Glu Gly Gln Glu Glu Ser Lys Glu Glu Gly Lys Glu Asp Leu Val
435 440 445
Glu Ser Glu Ser Lys Pro Glu Pro Thr Pro Glu Pro Glu Pro Glu Arg
450 455 460
Leu Ser Phe Gly Asn Phe Ile Arg Phe Arg Asn Pro Leu Ser His Leu
465 470 475 480
Ser Ser Lys Asn Leu Thr Ile Pro Ala Ser Ile Asp Tyr Leu Phe Glu
485 490 495
Gln Ala Pro Glu Trp Phe Lys Asn Arg Thr Leu Asn His Tyr Ser Tyr
500 505 510
Gly Leu Ala Arg Thr Arg Lys Glu Val Lys Ala Asn Asn Asp Asp Pro
515 520 525
Ser Lys Trp Ser Asn Pro Leu Glu Val Ala Leu Pro Asn Ala Pro Asp
530 535 540
Met Glu Ile Tyr Cys Phe Tyr Gly Val Gly Lys Pro Thr Glu Arg Ser
545 550 555 560
Tyr Tyr Tyr Gln Glu Glu Val Asp Lys Asp Leu Val Asn Leu Asn Ile
565 570 575
Ser Ile Ala His Asn Asp Pro Glu Ala Val Ile Met Gly Glu Gly Asp
580 585 590
Gly Thr Ile Ser Leu Asn Thr His Thr Met Cys His Arg Trp Lys Asp
595 600 605
Pro Asn Ser Lys Phe Asn Pro Gly Gly Ser Lys Val Lys Val Val Glu
610 615 620
Met Leu His Gln Pro Ala Thr Leu Asp Ile Arg Gly Gly Ala Lys Thr
625 630 635 640
Ala Glu His Val Asp Ile Leu Gly Arg Thr Glu Leu Asn Glu Leu Val
645 650 655
Leu Arg Val Ala Ala Gly Arg Gly Asp Glu Val Glu Glu Arg Ile Val
660 665 670
Ser Asn Ile Asp Ser Trp Val Trp Asp Ile Asp Leu Gly Ser Asp
675 680 685
<210> 106
<211> 2064
<212> DNA
<213> 噬腺嘌呤阿克苏拉酵母
<400> 106
atggctgtga agcgtcggag caacctcccg cagcaggagg ttgatagcga ttcatcgagt 60
gatagctcgt cggtgtcgag ctctgctgcc agcagctcgg tagatctggc gacgggttcg 120
tcatcgcaaa tcgaagatag gttccctaaa aagactgcaa gcgcgacaaa gcgaagctct 180
caccatcgca agagtaagaa agatggtgga cacttgagct acaagtggcg tttggcatat 240
tccaagcgat tggtgttcat tatcggaatc atttttgggc tgggaattgc gtggtactcg 300
gctccgaagg agtttgtgtc attagatagg ctgtcggagc tgtcgttaga cgggctgtta 360
gacgagttta gagatatgct gcccaagggg atcatgagag aggcccacga tattgataaa 420
aagtcgtata cgctgtccga ttcgtttgct gtgggcaatt acctgcggga agaagggtac 480
ggggtgaagc atcctgtgat cttgatccca ggagtgattt ccactgggct ggagtcatgg 540
ggtctggagg ggactgaaga gtgtccctca caaccccatt tccgtaaaag actatggggg 600
tcattgtata tgctgcgaac aatgcttctg gataagcatt gttggctcaa gcacattatg 660
ctggaccctt ctactgggct agatcctccc gggtataaga tacgtgctgc tttgggaatg 720
gaatcagccg atttctttgt cccggggtac tggttgtgga acaagatttt ggaaaacctg 780
gccgccatgg ggtacgattc caataacatg cttgtagctt cttacgactg gcgtctgtcg 840
taccctgatt tggagcgccg tgatagctat tttagtcgcc tcaagagtgc aattgagcat 900
tctgtacata gtactggcga aaaagtcgct ctggtaggcc attccatggg tactcaggtg 960
attttctact ttttaaagtg ggcagaagct aaaggctacg gtgatggagg agatcaatgg 1020
gtcaatgacc atattgcttc tctggttgat atttccggat ctaccttggg tactcccaag 1080
gccattgtgg ccctgctttc tggtgaaatg aaggatactg tccagcttaa tgcgctggct 1140
gtgtatggat tggaaaagtt cttttcccgc cgcgaacgag ctgatatgct gcggtcattt 1200
ggtggaattg cttctatgct tccaaagggt ggagaggcag tttggggtaa cttgacgttt 1260
gctccagatg accctcccat tactcctgcc gacgctcaag agggccaaga agagagcaaa 1320
gaagagggca aagaggacct cgttgagagt gagagtaagc ctgaacctac gcctgaacct 1380
gaacctgaaa gactttcttt tggaaatttc attcgattcc gcaaccctct gagccacttg 1440
tcaagtaaga acctgaccat tcccgcgtca attgactact tgtttgagca ggctcctgag 1500
tggttcaaaa atcgaactct gaaccattat tcatacgggc ttgctcgcac ccgaaaagag 1560
gtaaaggcca acaatgacga cccttctaaa tggtccaatc ctttggaagt tgcattgcca 1620
aacgctccag acatggaaat ctattgcttc tacggagtgg gcaaacctac tgaacgcagc 1680
tactactacc aagaagaagt cgacaaggat ttggtcaacc tcaatatcag tattgctcat 1740
aatgatcccg aggctgtgat aatgggtgag ggtgatggaa caatctccct caacacccac 1800
acaatgtgcc accgctggaa ggacccaaac tcaaagttca accccggagg cagcaaagta 1860
aaagtagtgg agatgctcca tcagcctgcc actctagata ttcgtggagg cgcaaagacc 1920
gcggagcacg tggatatctt gggccgtacc gagctcaacg agctggtcct tcgggtcgct 1980
gctggtcgag gtgatgaagt cgaagagcgc atcgtgagca acattgacag ctgggtgtgg 2040
gacatcgacc ttggtagtga ttaa 2064
<210> 107
<211> 331
<212> PRT
<213> 噬腺嘌呤阿克苏拉酵母
<400> 107
Met Thr Lys Leu Ser Tyr Val Gly Arg Pro Pro Thr Asp Ile Phe Gln
1 5 10 15
Trp Pro Val Gly Leu Ala Met Pro Ser Gly Pro Phe Gly Ser Trp Phe
20 25 30
Glu Thr Met Met Asp Val Arg Val Pro Leu Thr Val Ala Ser Val Tyr
35 40 45
Ala Thr Thr Val His Val Leu Asn His Phe Arg Lys Thr Asn Lys Glu
50 55 60
Pro Ile Gly Leu Ala Lys Thr Arg Leu Phe Gln Trp Leu Val Val Ala
65 70 75 80
His Asn Met Gly Leu Cys Val Tyr Ser Ala Trp Thr Cys Tyr Gly Met
85 90 95
Ser Ala Ala Ile Tyr Gln Ser Val Phe Glu Val Thr Lys Val Ala Val
100 105 110
Gly Asn Thr Glu Arg Gly Thr Val Asp Ala Tyr Leu Arg Gly Glu Gly
115 120 125
Ser Leu Val Asp Gly Thr Ser Gly Asn Ala Thr Ala Gly Phe Trp Arg
130 135 140
Ala Leu Cys Asp Val Asp Val Gly Ile Trp Asp His Gly Leu Ser Tyr
145 150 155 160
Tyr Gly Phe Phe Phe Tyr Leu Ser Lys Phe Tyr Glu Val Val Asp Thr
165 170 175
Leu Ile Ile Leu Ala Lys Gly Lys Gln Ser Ser Leu Leu Gln Thr Tyr
180 185 190
His His Ala Gly Ala Met Leu Ser Met Trp Ala Gly Ile Arg Phe Ala
195 200 205
Ser Pro Pro Ile Trp Ile Phe Val Val Phe Asn Ser Leu Ile His Thr
210 215 220
Ile Met Tyr Phe Tyr Tyr Thr Leu Thr Thr Leu Lys Leu Arg Val Pro
225 230 235 240
Thr Val Val Lys Arg Ser Leu Thr Thr Ala Gln Ile Cys Gln Phe Val
245 250 255
Phe Gly Gly Ser Phe Ala Leu Leu His Met Phe Val Tyr Tyr Phe Asp
260 265 270
Pro Asn Thr Gln Ser Tyr Ser Ser Cys Leu Ser Asp Pro Gly Gln Gly
275 280 285
Phe Ala Leu Met Phe Asn Val Ala Tyr Leu Ala Pro Leu Thr Tyr Leu
290 295 300
Phe Val Asn Phe Trp Ile Asp Ser Tyr Val Arg Ser Lys Gln Arg Ala
305 310 315 320
Ala Asp Arg Lys Ala Ala Ala Lys Ala Lys Gln
325 330
<210> 108
<211> 996
<212> DNA
<213> 噬腺嘌呤阿克苏拉酵母
<400> 108
atgaccaagc tgtcgtacgt gggacgcccg ccaactgaca tttttcaatg gccagtaggc 60
ctggcaatgc catccggtcc gtttggatct tggttcgaga ctatgatgga tgtacgagtg 120
ccgttgacgg ttgcctctgt gtacgctaca actgttcatg tgctgaacca tttccgaaag 180
accaacaagg agcccattgg cctggcaaag actcgattgt tccagtggtt ggtcgtggcc 240
cacaacatgg gcctgtgtgt ttactctgcc tggacttgtt atggtatgtc tgcagcaatt 300
taccagtctg tgtttgaggt gaccaaggta gcagtcggaa acactgagcg tggtactgtt 360
gatgcgtacc tgcgtggaga gggtagtctg gtcgatggaa ccagcggtaa cgctactgcc 420
ggattctggc gagccttgtg cgatgtcgat gttggtatct gggaccacgg actgtcctac 480
tatggatttt tcttctatct ttcaaagttt tacgaggtgg tcgacactct gatcattctg 540
gccaagggta agcagtcgtc tcttcttcag acttatcacc acgccggtgc tatgttgtcc 600
atgtgggctg gaattcgatt cgcttcccct cccatttgga tctttgtggt gttcaattct 660
ctcattcaca ccatcatgta tttctactac accttgacca cccttaagct gcgcgttcct 720
accgtggtga agcgcagttt gaccaccgcc cagatttgcc aattcgtctt cggtggttcg 780
tttgcactgc tgcacatgtt tgtgtactat tttgatccca acacccaatc gtactcatcc 840
tgtctgtccg atccgggcca aggatttgcc ctcatgttca atgtagccta tctggcccca 900
ttgacttatc tattcgtcaa cttttggatt gacagttacg tgcgaagcaa gcagcgggcc 960
gccgaccgca aagctgcggc aaaggcaaag cagtaa 996
<210> 109
<211> 648
<212> PRT
<213> 解脂耶氏酵母
<400> 109
Met Thr Gln Pro Val Asn Arg Lys Ala Thr Val Glu Arg Val Glu Pro
1 5 10 15
Ala Val Glu Val Ala Asp Ser Glu Ser Glu Ala Lys Thr Asp Val His
20 25 30
Val His His His His His His His Lys Arg Lys Ser Val Lys Gly Lys
35 40 45
Ile Leu Asn Phe Phe Thr Arg Ser Arg Arg Ile Thr Phe Val Leu Gly
50 55 60
Ala Val Val Gly Val Ile Ala Ala Gly Tyr Tyr Ala Ala Pro Pro Glu
65 70 75 80
Leu Ser Ile Asp Ile Asp Ala Leu Leu Gly Asp Leu Pro Ser Phe Asp
85 90 95
Phe Asp Ala Leu Ser Leu Asp Asn Leu Ser Met Asp Ser Val Ser Asp
100 105 110
Phe Val Gln Asp Met Lys Ser Arg Phe Pro Thr Lys Ile Leu Gln Glu
115 120 125
Ala Ala Lys Ile Glu Lys His Gln Lys Ser Glu Gln Lys Ala Ala Pro
130 135 140
Phe Ala Val Gly Lys Ala Met Lys Ser Glu Gly Leu Asn Ala Lys Tyr
145 150 155 160
Pro Val Val Leu Val Pro Gly Val Ile Ser Thr Gly Leu Glu Ser Trp
165 170 175
Ser Leu Glu Gly Thr Glu Glu Cys Pro Thr Glu Ser His Phe Arg Lys
180 185 190
Arg Met Trp Gly Ser Trp Tyr Met Ile Arg Val Met Leu Leu Asp Lys
195 200 205
Tyr Cys Trp Leu Gln Asn Leu Met Leu Asp Thr Glu Thr Gly Leu Asp
210 215 220
Pro Pro His Phe Lys Leu Arg Ala Ala Gln Gly Phe Ala Ser Ala Asp
225 230 235 240
Phe Phe Met Ala Gly Tyr Trp Leu Trp Asn Lys Leu Leu Glu Asn Leu
245 250 255
Ala Val Ile Gly Tyr Asp Thr Asp Thr Met Ser Ala Ala Ala Tyr Asp
260 265 270
Trp Arg Leu Ser Tyr Pro Asp Leu Glu His Arg Asp Gly Tyr Phe Ser
275 280 285
Lys Leu Lys Ala Ser Ile Glu Glu Thr Lys Arg Met Thr Gly Glu Lys
290 295 300
Thr Val Leu Thr Gly His Ser Met Gly Ser Gln Val Ile Phe Tyr Phe
305 310 315 320
Met Lys Trp Ala Glu Ala Glu Gly Tyr Gly Gly Gly Gly Pro Asn Trp
325 330 335
Val Asn Asp His Ile Glu Ser Phe Val Asp Ile Ser Gly Ser Met Leu
340 345 350
Gly Thr Pro Lys Thr Leu Val Ala Leu Leu Ser Gly Glu Met Lys Asp
355 360 365
Thr Val Gln Leu Asn Ala Met Ala Val Tyr Gly Leu Glu Gln Phe Phe
370 375 380
Ser Arg Arg Glu Arg Ala Asp Leu Leu Arg Thr Trp Gly Gly Ile Ala
385 390 395 400
Ser Met Ile Pro Lys Gly Gly Lys Ala Ile Trp Gly Asp His Ser Gly
405 410 415
Ala Pro Asp Asp Glu Pro Gly Gln Asn Val Thr Phe Gly Asn Phe Ile
420 425 430
Lys Phe Lys Glu Ser Leu Thr Glu Tyr Ser Ala Lys Asn Leu Thr Met
435 440 445
Asp Glu Thr Val Asp Phe Leu Tyr Ser Gln Ser Pro Glu Trp Phe Val
450 455 460
Asn Arg Thr Glu Gly Ala Tyr Ser Phe Gly Ile Ala Lys Thr Arg Lys
465 470 475 480
Gln Val Glu Gln Asn Glu Lys Arg Pro Ser Thr Trp Ser Asn Pro Leu
485 490 495
Glu Ala Ala Leu Pro Asn Ala Pro Asp Leu Lys Ile Tyr Cys Phe Tyr
500 505 510
Gly Val Gly Lys Asp Thr Glu Arg Ala Tyr Tyr Tyr Gln Asp Glu Pro
515 520 525
Asn Pro Glu Gln Thr Asn Leu Asn Val Ser Ile Ala Gly Asn Asp Pro
530 535 540
Asp Gly Val Leu Met Gly Gln Gly Asp Gly Thr Val Ser Leu Val Thr
545 550 555 560
His Thr Met Cys His Arg Trp Lys Asp Glu Asn Ser Lys Phe Asn Pro
565 570 575
Gly Asn Ala Gln Val Lys Val Val Glu Met Leu His Gln Pro Asp Arg
580 585 590
Leu Asp Ile Arg Gly Gly Ala Gln Thr Ala Glu His Val Asp Ile Leu
595 600 605
Gly Arg Ser Glu Leu Asn Glu Met Val Leu Lys Val Ala Ser Gly Lys
610 615 620
Gly Asn Glu Ile Glu Glu Arg Val Ile Ser Asn Ile Asp Glu Trp Val
625 630 635 640
Trp Lys Ile Asp Leu Gly Ser Asn
645
<210> 110
<211> 1947
<212> DNA
<213> 解脂耶氏酵母
<400> 110
atgacacaac ctgtgaatcg gaaggcgact gtcgagcggg tcgagccagc agtggaggtg 60
gctgactccg agtccgaggc caagaccgac gtccacgttc accaccatca tcaccaccac 120
aagcgaaaat ccgtcaaggg caagattctc aacttcttca cccgaagtcg acgtatcacc 180
ttcgtcctcg gcgccgtggt cggtgtgata gccgcgggat actacgctgc gccaccggag 240
ctcagcattg atatcgatgc tcttctcggc gacttgccct cgttcgactt tgacgctcta 300
tctctcgaca acttgtccat ggacagtgtg tcggactttg tacaagacat gaaatcgcgg 360
tttccgacca agattctgca ggaggcggcc aagatcgaga agcaccagaa aagcgaacag 420
aaggctgccc cttttgctgt gggcaaggct atgaagagcg agggactcaa cgccaagtac 480
ccggtggtgc tggtgcccgg cgtcatctcc acgggactgg agagctggtc cctggaggga 540
accgaggagt gtcccaccga gtcgcacttc agaaagcgaa tgtggggctc ctggtacatg 600
atccgagtca tgctgctgga caagtactgc tggctgcaga acctgatgct ggacacagag 660
accggtctag accctcccca tttcaagctg cgagccgccc agggatttgc ctccgccgac 720
ttctttatgg caggctactg gctgtggaac aagctgctcg agaacctggc tgttattgga 780
tacgatacgg atacaatgtc tgctgcggcg tacgactgga gactgtccta ccctgatttg 840
gagcaccgag acggatactt ctccaagctc aaagcttcaa tcgaagagac taagcgtatg 900
acaggtgaga agacagttct gacgggccat tccatgggct cccaggtcat cttctacttc 960
atgaagtggg ctgaggccga gggatatgga ggaggaggtc ccaactgggt caatgaccat 1020
attgaatcct ttgtcgacat ttccggctcc atgctgggta ctcccaagac cctggttgct 1080
cttctgtctg gagaaatgaa ggataccgtg cagctgaacg cgatggctgt gtatggactg 1140
gagcagttct tctctcgacg agagcgagcc gatctgctgc gaacatgggg aggaattgct 1200
tccatgattc ccaagggtgg taaggctatc tggggtgatc attctggagc ccctgatgac 1260
gagcctggcc agaatgtcac ctttggcaac ttcatcaagt tcaaggagtc cttgaccgag 1320
tactctgcta agaacctcac catggatgaa accgttgact tcctgtattc tcagtctccc 1380
gagtggtttg tgaaccgaac cgagggtgct tactcctttg gaattgccaa gactcgaaag 1440
caggttgagc agaatgagaa gcgaccttct acctggagca accctctgga agctgctctc 1500
cccaatgccc ccgatctcaa gatctactgc ttctatggag tcggtaagga taccgagcga 1560
gcctactact accaggatga gcccaatccc gagcagacca acttgaacgt cagtatcgct 1620
ggaaacgacc ctgatggtgt gcttatgggc cagggcgatg gaaccgtctc ccttgtgacc 1680
cataccatgt gtcaccgatg gaaggacgag aattccaagt tcaaccctgg taacgcccag 1740
gtcaaggttg tggagatgtt gcaccagcct gatcgacttg atattcgagg cggtgctcag 1800
actgccgagc atgtggacat tctggggcgt tctgagttga acgagatggt tctgaaggtg 1860
gccagtggaa agggaaatga gattgaagag agagtcatct ccaacattga tgagtgggtg 1920
tggaagattg atctcggcag caattag 1947
<210> 111
<211> 533
<212> PRT
<213> 粘红酵母
<400> 111
Met Thr Ala Ser Ser Ala Leu Glu Thr Ser Leu Pro His Ser Val Gly
1 5 10 15
Pro Glu Ser Ala Thr Thr Thr Ala Lys Pro Pro Arg Ala Pro Leu Arg
20 25 30
Met Arg His Pro Asp Tyr Thr Gln Thr Asp Val Leu Glu Ser Ser Asp
35 40 45
Ser Asp Ala Ala Ser Asp Ser Glu Gly Glu Thr Thr Ala Val Asp Asp
50 55 60
Gly Thr Tyr Glu Asp Asp Asn Tyr Val Arg Lys Val Leu Ser Lys Glu
65 70 75 80
Lys Pro Leu Pro Pro Ile Thr Trp Lys Asn Ile His Arg Asn Ile Gln
85 90 95
Trp Ile Ser Thr Leu Ala Leu Thr Ile Val Pro Leu Leu Ser Ile Tyr
100 105 110
Gly Ala Phe Thr Thr Pro Leu Lys Trp Gln Thr Ala Val Trp Ser Val
115 120 125
Val Tyr Tyr Tyr Phe Thr Gly Leu Gly Ser Tyr Thr Ala Ser Leu Pro
130 135 140
Leu Gln Tyr Phe Leu Ala Leu Gly Gly Ser Gly Ala Val Glu Gly Ser
145 150 155 160
Val Lys Trp Trp Ala Arg Gly His Arg Ala His His Arg Tyr Thr Asp
165 170 175
Thr Asp Leu Asp Pro Tyr Ser Ala Gln Lys Gly Phe Trp Trp Ala His
180 185 190
Leu Gly Trp Met Ile Val Lys Pro Arg Arg Arg Pro Gly Val Ala Asp
195 200 205
Val Ser Asp Leu Asn Asn Asn Pro Val Val Lys Trp Gln His Arg Phe
210 215 220
Tyr Leu Pro Leu Ile Leu Gly Met Gly Phe Ile Phe Pro Thr Ile Val
225 230 235 240
Ala Gly Leu Gly Trp Gly Asp Phe Arg Gly Gly Phe Phe Phe Ala Gly
245 250 255
Ala Ala Arg Leu Leu Phe Val His His Ser Thr Phe Cys Val Asn Ser
260 265 270
Leu Ala His Trp Leu Gly Glu Thr Pro Phe Asp Asp Lys His Thr Pro
275 280 285
Lys Asp His Trp Leu Thr Ala Leu Ala Thr Val Gly Glu Gly Tyr His
290 295 300
Asn Phe His His Glu Phe Pro Ser Asp Tyr Arg Asn Ala Leu Arg Trp
305 310 315 320
Trp Gln Tyr Asp Pro Thr Lys Cys Phe Ile Tyr Ala Met Ser Lys Leu
325 330 335
Gly Leu Ala Ser Gln Leu Lys Thr Phe Pro Asp Asn Glu Ile Lys Lys
340 345 350
Gly Gln Tyr Ala Met Thr Leu Lys Ala Val Ala Arg Glu Ala Glu Asn
355 360 365
Ile Glu Trp Pro Lys Ser Ser Asn His Leu Pro Val Leu Thr Trp Asp
370 375 380
Glu Phe Gln Glu Ala Cys Lys Thr Arg Gln Leu Leu Val Val Ala Gly
385 390 395 400
Phe Ile His Asp Val Ser Thr Phe Ile Asp Gln His Pro Gly Gly Ala
405 410 415
Gly Leu Ile Lys Thr Arg Leu Gly Arg Asp Ala Thr Thr Ala Phe Tyr
420 425 430
Gly Gly Tyr Tyr Asp His Ser Asn Gly Ala Ala Asn Leu Leu Ala Gln
435 440 445
Tyr Arg Val Gly Val Ile Glu Gly Gly Tyr Glu Val Glu His Met Lys
450 455 460
Lys Tyr Ser Glu Val Val Glu Asn Leu Lys Lys His Gly Ala Asp Gly
465 470 475 480
Val Ala Gly Lys Ser Ala Asp Leu Ala Lys Gly Pro Lys Gln Met Ser
485 490 495
Val Ile Lys Gly Asp Pro Gln Leu Lys Gly Ala Pro Leu Glu Thr Leu
500 505 510
Ala Lys Pro Pro Thr Phe Ser Glu Thr Asn Leu Leu Gly Gly Leu Ser
515 520 525
Leu Thr Val Lys Ala
530
<210> 112
<211> 1602
<212> DNA
<213> 粘红酵母
<400> 112
atgactgcct cgtcggcact cgagacctcg ctcccgcact ctgtcgggcc cgagtctgcg 60
actaccaccg caaagccgcc ccgtgcgccg ctcaggatgc gtcaccccga ctacacccag 120
accgacgtcc tcgagtcgtc agactcggac gcagcgtcgg attccgaggg cgagacgacg 180
gctgtcgacg acgggaccta cgaggacgat aactacgtcc gcaaggtcct cagcaaggag 240
aagccgctcc cgcccatcac ctggaagaac atccaccgca acatccagtg gatctcgacc 300
ctcgccctca ccatcgtgcc cctcctctcg atctacggag cgttcacgac gcccctgaag 360
tggcagacgg cggtctggag tgtcgtctac tactacttca ccggtctcgg gtcctacact 420
gcctccctgc ctctccagta cttcctggca cttggcggaa gcggcgcagt ggagggttct 480
gtgaaatggt gggcccgagg acaccgcgca caccaccgct acaccgacac ggacctcgac 540
ccgtactcag cgcagaaggg cttctggtgg gcacacctcg gctggatgat tgtcaagccg 600
cgccgtcgtc ccggtgtcgc cgatgtctcc gacctcaaca acaacccagt cgtcaagtgg 660
cagcaccgct tctacctccc gctcatcctc ggcatgggct tcatcttccc taccatcgtc 720
gctggactcg gctggggcga cttccgcggc ggatttttct tcgccggcgc tgctcgcctc 780
ctctttgtcc accactcgac gttctgcgtc aactcgctcg cacactggct cggcgagacg 840
ccgtttgacg acaagcacac gccgaaggac cactggctca ccgcgctcgc gacggtcggc 900
gagggctacc acaacttcca ccacgagttc ccctccgact accgcaacgc gctcagatgg 960
tggcagtatg atccgactaa gtgtttcatt tacgcgatgt cgaaactcgg attggcgtcg 1020
cagctcaaga cgttccccga caacgagatc aagaagggtc agtacgccat gacgctcaag 1080
gctgtcgcgc gcgaggcgga gaacatcgag tggcccaagt cgtcgaacca cttgcctgtg 1140
ctcacctggg atgagttcca ggaggcctgc aagactcgcc agctcctcgt tgtcgccggt 1200
ttcatccacg atgtcagcac cttcatcgac cagcaccctg gcggtgccgg cttgatcaag 1260
acccgtctcg gccgcgatgc gacgaccgcc ttctacggtg gctactacga ccactcgaac 1320
ggcgcagcca acttgctcgc ccagtaccgt gtcggcgtca tcgagggcgg ctacgaggtc 1380
gagcacatga agaagtactc tgaggtcgtc gagaacctca agaagcacgg cgccgacggc 1440
gtggccggca agagcgccga cctcgccaag ggtccgaagc agatgtcggt catcaagggc 1500
gaccctcagc tcaagggcgc gccgctcgag acgctcgcca agccgcctac cttcagcgag 1560
accaaccttt tgggcggtct cagcctgacg gtcaaggcgt aa 1602
<210> 113
<211> 579
<212> PRT
<213> 禾本红酵母
<400> 113
Met Arg His Pro Asp Tyr Ser Gln Gly Asp Val Val Glu Ser Ser Asp
1 5 10 15
Ser Asp Arg Glu Ala Ser Asp Ser Glu Gly Glu Thr Thr Ala Val Asp
20 25 30
Asp Gly Thr Tyr Gln Asp Asp Asn Phe Val Arg Lys Val Leu Ala Lys
35 40 45
Glu Arg Pro Leu Pro Pro Ile Thr Leu Lys Thr Leu Pro Gln Asn Ile
50 55 60
Asn Val Ile Ser Thr Leu Ala Leu Thr Val Val Pro Ala Leu Ala Ile
65 70 75 80
Tyr Gly Ala Phe Thr Thr Gln Ile Lys Trp Gln Thr Ala Leu Trp Ser
85 90 95
Val Ile Tyr Tyr Phe Tyr Thr Gly Leu Gly Ile Thr Ala Gly Tyr His
100 105 110
Arg Leu Trp Ala His Arg Ser Tyr Thr Ala Ser Leu Pro Leu Gln Tyr
115 120 125
Phe Leu Ala Leu Gly Gly Ser Gly Ala Val Glu Gly Ser Ile Lys Trp
130 135 140
Trp Ser Arg Gly His Arg Ala His His Arg Tyr Thr Asp Thr Asp Leu
145 150 155 160
Asp Pro Tyr Ser Ala Gln Lys Gly Phe Trp Trp Ser His Ile Gly Trp
165 170 175
Met Val Val Lys Pro Arg Arg Thr Pro Gly Val Ala Asp Val Ser Asp
180 185 190
Leu Ser Val Asn Glu Val Val Lys Trp Gln His Arg Trp Tyr Val Tyr
195 200 205
Leu Ile Val Gly Met Gly Phe Val Phe Pro Thr Leu Val Ala Gly Leu
210 215 220
Gly Trp Gly Asp Tyr Arg Gly Gly Phe Phe Phe Ala Gly Ala Ala Arg
225 230 235 240
Leu Leu Phe Val His His Ser Thr Phe Cys Val Asn Ser Leu Ala His
245 250 255
Trp Leu Gly Glu Thr Pro Phe Asp Asp Lys His Ser Pro Arg Asp His
260 265 270
Trp Ile Thr Ala Leu Val Thr Val Gly Glu Gly Tyr His Asn Phe His
275 280 285
His Glu Phe Pro Gln Asp Phe Arg Asn Ala Ile Gln Thr Phe Gln Tyr
290 295 300
Asp Pro Thr Lys Trp Phe Ile Ile Val Met His Trp Leu Gly Leu Ala
305 310 315 320
Ser Gln Leu Lys Thr Phe Pro Asp Asn Glu Ile Arg Arg Gly Gln Tyr
325 330 335
Ala Met Lys Leu Lys Ala Val Ala Arg Glu Ala Asp Glu Ile Arg Trp
340 345 350
Pro Lys Ser Ser Asn His Leu Pro Val Leu Thr Trp Asp Glu Phe Gln
355 360 365
Glu Ala Cys Lys Thr Arg Gln Leu Met Val Ile Ser Gly Tyr Ile His
370 375 380
Asp Val Ser Thr Phe Ile Asp Glu His Pro Gly Gly Arg Ala Leu Ile
385 390 395 400
Lys Thr Arg Leu Gly Arg Asp Ala Thr Asn Ala Phe Tyr Gly Gly Tyr
405 410 415
Tyr Asp His Ser Asn Gly Ala Asn Asn Val Leu Ala Gln Tyr Arg Val
420 425 430
Gly Val Ile Glu Gly Gly Tyr Glu Val Glu His Leu Lys Arg Phe Ser
435 440 445
Lys Leu Ile Glu Asp Leu Lys Asp Ser Ser Ala Thr Ser Pro Ala Pro
450 455 460
Ser Ala Ser Thr Pro Ser Ser Thr Leu Ser Ser Ala Cys Thr Pro Ser
465 470 475 480
Thr Ser Thr Cys Thr Ser Pro Thr Ser Arg Ser Thr Arg Ser Ser Pro
485 490 495
Pro Ser Pro Ser Ala Pro Thr Pro Thr Ser Ser Ser Thr Leu Pro Arg
500 505 510
Arg Ala Arg Cys Thr Ser Arg Arg Ser Arg Arg Ser Ser Ser Arg Arg
515 520 525
Ser Arg Arg Ser Arg Ser Ser Pro Arg Ala Ser Pro Ser Ala Thr Arg
530 535 540
Ala Arg Ser Ser Thr Pro Pro Ser Arg Arg Arg Ser Ser Ser Ser Ala
545 550 555 560
Arg Arg Arg Ser Ala Ala Ser Ser Arg Ala Ala Ser Ala Ser Ala Thr
565 570 575
Arg Ala Ala
<210> 114
<211> 1740
<212> DNA
<213> 禾本红酵母
<400> 114
atgcgccacc cggactactc gcagggcgac gttgtcgagt cgtccgactc ggaccgcgag 60
gcgagcgact cggagggcga gacgaccgcc gtcgacgacg gcacctacca ggacgacaac 120
tttgtccgca aggtgctcgc caaggagcgt cctctgccgc ccatcacgct caagacgctc 180
ccgcagaaca tcaacgtcat ctcgaccctc gccctcaccg tcgtccccgc cctcgccatc 240
tacggcgcgt tcacgacgca gatcaagtgg cagacggctt tgtggagcgt catctactac 300
ttctacactg gcctcggtat cacggccggg taccaccgcc tgtgggctca ccgcagctac 360
acggcgtctt tgccgctcca gtacttcctc gctctcggag gatctggcgc tgtcgagggc 420
tcgatcaagt ggtggagccg tggtcaccgt gcgcaccacc gctacaccga caccgacctc 480
gacccgtact cggcgcagaa gggcttctgg tggtcgcaca tcggctggat ggtcgtcaag 540
ccccgtcgca cgcccggtgt cgccgacgtc agcgacctgt cggtcaacga ggtcgtcaag 600
tggcagcacc ggtggtacgt gtacctcatc gtcggcatgg gcttcgtctt cccgaccctc 660
gtcgccggcc tcggctgggg cgactaccgc ggcggcttct tcttcgccgg cgccgctcgc 720
ctcctgttcg tccaccactc gaccttctgc gtcaactcgc tcgcccactg gctcggtgag 780
acgccgttcg acgacaagca ctcgccgcgc gaccactgga tcacggcgct cgtcaccgtc 840
ggcgagggct accacaactt ccaccacgag ttcccccagg acttccgcaa cgcgatccag 900
accttccagt acgacccgac caagtggttc atcatcgtca tgcactggct cggcctcgcg 960
tcgcagctca agacgttccc cgacaacgag attcgccgcg gccagtacgc catgaagctc 1020
aaggccgtcg ctcgcgaggc cgacgagatc cgctggccca agtcgtccaa ccacctcccc 1080
gtcctcacgt gggacgagtt ccaggaggcg tgcaagactc gccagctcat ggtcatctcg 1140
ggctacatcc acgacgtctc gaccttcatc gacgagcacc ctggcggccg tgcgctcatc 1200
aagacccgcc tcggccgtga cgcgaccaac gccttctacg gtggctacta cgaccactcg 1260
aacggcgcca acaacgtcct cgcgcagtac cgcgtcggcg tcatcgaggg cggctacgag 1320
gtcgagcacc tcaagcgctt ctccaagctc atcgaggacc tcaaggactc ctccgcgacg 1380
tcaccggcgc cgagcgcctc gacgccctcg tcgactttgt cgtccgcctg tacgccgtct 1440
acgtcgacct gcacttcgcc tacctcgaga tcaacccgct cgtcgccgcc gtcgccaagt 1500
gccccgacgc cgacgtcgtc gtcaactttg cctcgtcgcg ctcggtgtac cagtcgacgc 1560
tcgaggcgct cgagttctcg cagatcaagg cgatcgcgct catcgccgag ggcgtccccg 1620
agcgccacgc gcgcgagatc ctccacgccg ccgagcagaa gaaggtcatc atcatcggcc 1680
cggcgacggt cggcggcatc aagccgggct gcttccgcat cggcaacacg ggcggcatga 1740
<210> 115
<211> 750
<212> PRT
<213> 禾本红酵母
<400> 115
Met Phe Leu His Ala Arg Gly Ala Leu Gly Ser Asn Ile Val Tyr Asp
1 5 10 15
Val Val Ala Ser Met Trp Arg Ile Val Ile Thr Leu Phe Phe Arg Glu
20 25 30
Ile Arg Ser Arg Gly Ala Trp Lys Ile Pro Arg Ala Ala Glu Gly Pro
35 40 45
Val Ile Phe Val Val Gly Pro His His Asn Gln Phe Leu Asp Pro Leu
50 55 60
Leu Leu Met Ser Glu Val Lys Arg Glu Ser Gly Arg Arg Ile Ser Phe
65 70 75 80
Leu Thr Ala Ala Lys Ser Met Asp Arg Ala Phe Val Gly Leu Ala Ser
85 90 95
Arg Leu Met Gln Ser Ile Pro Val Ala Arg Ala Gln Asp Ser Ala Phe
100 105 110
Ala Gly Lys Gly Thr Ile Lys Leu Ser Asp Ser Asp Pro Cys Ile Ile
115 120 125
Ile Gly Glu Gly Thr Ser Phe Thr Lys Asp Phe Glu Lys Pro Arg Ser
130 135 140
Gln Val Leu Leu Pro Arg Asn Leu Gly Ser Ser Thr Ala Glu Val Val
145 150 155 160
Glu Val Ile Ser Asp Thr Glu Leu Arg Leu Lys Lys Glu Phe Asn Arg
165 170 175
Lys Ala Thr Glu Gly Leu Lys Glu Lys Ser Glu Gly Ser Ser Phe Lys
180 185 190
Val Leu Pro His Val Asp Gln Thr Ser Met Tyr Ser Ala Val Tyr Gln
195 200 205
Arg Leu Thr Glu Gly Gly Cys Ile Gly Ile Phe Pro Glu Gly Gly Ser
210 215 220
His Asp Arg Thr Asp Leu Leu Pro Leu Lys Ala Gly Val Ser Ile Met
225 230 235 240
Ala Leu Gly Ala Ile Ser Ala His Pro Asp Leu His Leu Gln Ile Val
245 250 255
Pro Val Gly Leu Ser Tyr Phe His Pro His Lys Phe Arg Ser Arg Ala
260 265 270
Val Val Glu Phe Gly Ser Pro Ile Glu Ile Pro Thr Glu Leu Val Arg
275 280 285
Asp Phe Glu Gln Gly Gly Asp Ser Lys Lys Lys Ala Ile Ser Thr Val
290 295 300
Met Asp Tyr Val Val Asn Gly Leu Lys Ser Val Thr Ile Arg Ala Pro
305 310 315 320
Asp Tyr Asp Thr Leu Met Leu Val Gln Ala Ala Arg Arg Leu Tyr Arg
325 330 335
Pro Pro Gly Gln Asn Leu Thr Leu Gly Gln Val Val Glu Leu Asn Lys
340 345 350
Arg Phe Ile Val Gly Tyr Asp Ala Tyr Lys Glu Asp Pro Arg Ile Lys
355 360 365
Asn Leu Glu His Arg Val Arg Glu Tyr Asn Thr Leu Leu Arg Tyr Met
370 375 380
Gly Leu Lys Asp His Gln Val Asp Arg Ile Gly Arg Pro Arg Trp Arg
385 390 395 400
Ser Phe Ala Leu Leu Cys Tyr Arg Leu Gly Leu Leu Gly Val Trp Gly
405 410 415
Val Leu Ala Leu Pro Gly Val Val Leu Asn Ser Pro Ile Phe Leu Ala
420 425 430
Ala Lys Ile Ile Ser His Lys Lys Ala Lys Asp Ala Leu Ala Ala Ser
435 440 445
Ser Val Lys Ile Ala Gly Arg Asp Val Leu Ala Thr Trp Lys Val Leu
450 455 460
Val Ala Leu Gly Gly Ala Pro Ala Leu Tyr Ser Val Tyr Ala Val Asn
465 470 475 480
Ala Val Val Leu Ala His Lys Leu Ala Leu Pro Ala Ser Tyr Arg Phe
485 490 495
Trp Ala Pro Phe Ala Thr Phe Ala Gly Leu Pro Leu Ile Gly Val Ala
500 505 510
Ala Leu Lys Phe Gly Glu Val Gly Met Asp Val Tyr Lys Ser Leu Arg
515 520 525
Pro Leu Val Leu Ser Leu Val Pro Gly Lys Glu Pro Gln Leu Met Arg
530 535 540
Val Gln Arg Met Arg Glu Glu Leu Ala Ser Glu Leu Asn Leu Leu Val
545 550 555 560
Asp Glu Leu Ala Pro Ala Leu Phe Asp Asp Phe Ser Ala Ser Arg Ile
565 570 575
Ile Pro Ser Thr Thr Pro Val Pro Gln Arg Arg Asp Ser Ala Gln Gly
580 585 590
Lys Phe Phe Gln His Pro Leu Ser Trp Val Asp Glu Leu Phe Phe Gly
595 600 605
Pro Ala Trp Ser Ser Ser Met Ala Tyr Pro Gln Asp Arg His Val Arg
610 615 620
Pro Asn Gln Gly Glu Ser Leu His Ala Pro Ser Thr Gly Asn Gly Ala
625 630 635 640
Glu Ser Asp Leu Asp Gly Ala Phe Thr Asp Gly Gln Gly Thr Gly Ser
645 650 655
Gly Tyr Val Ser Gly Tyr Thr Thr Glu Glu Ala Pro Asp Tyr Asp Glu
660 665 670
Val Ile His Ile Leu Asn Arg Glu Gln Gly His Pro Asp Ser Pro Ala
675 680 685
Val Gly Ala Arg Pro Thr Ile Ser Arg Arg Ala Ser Arg Gln Arg Ser
690 695 700
Arg Ser Gly Leu Asn Leu Ala Ala Met Ser Pro Val Ser Pro Ala Thr
705 710 715 720
Pro Leu Ser Ser Ala Thr Ala Ser Gly Ala Gln Ala Gly Ser Ser Gly
725 730 735
Ala Glu Ala Arg Arg Arg Gly Ala Ala Pro Gln Gln Asp Glu
740 745 750
<210> 116
<211> 2253
<212> DNA
<213> 禾本红酵母
<400> 116
atgttcctcc atgctcgagg cgcgcttggc agcaacatcg tgtacgacgt cgtcgcgtcg 60
atgtggcgca tcgtcatcac gctcttcttc cgcgagatcc gctcgcgcgg cgcgtggaag 120
atcccgcgcg ccgccgaggg gcccgtcatc tttgtcgtcg gtcctcacca caaccagttc 180
ctcgacccgc tgctcctcat gagcgaggtc aagcgcgaga gcggtcggcg catcagcttt 240
ctcacggcgg ccaagagcat ggacagggcg ttcgtcgggc tcgcctcgag gctcatgcag 300
agcatccctg tcgcgcgcgc ccaggactcg gcatttgccg gcaagggcac gatcaagctc 360
tccgactcgg acccgtgcat catcatcggc gagggcacgt cgttcaccaa ggactttgag 420
aagccgcgct cccaggtcct cctgccgcgc aacctcggca gctcgacggc cgaggtcgtc 480
gaggtcatca gcgacaccga gctgcgcctc aagaaggagt tcaaccgcaa ggcgaccgag 540
ggcctcaagg agaagagcga gggctcgtct ttcaaggttc ttccgcatgt cgaccagacg 600
agcatgtact cggccgtgta ccagcgcctg accgagggcg ggtgcatcgg catcttcccc 660
gagggcggct cccacgaccg caccgacctc ctgccgctca aggccggcgt ctcgatcatg 720
gcgctcggcg ccatctcggc gcacccggac ctgcacctcc agatcgtccc cgtcggcctc 780
agctactttc acccgcacaa gttccgctcg cgcgccgtcg tcgagttcgg cagcccgatc 840
gagatcccga ccgagctcgt gcgcgacttt gagcagggcg gcgacagcaa gaagaaggcg 900
atcagcaccg tcatggatta cgtcgtcaac gggctcaaga gcgtcacgat ccgtgcgcct 960
gactacgaca cgctgatgct cgtccaagcc gcacgtcgcc tgtaccgccc gcccgggcag 1020
aacctgacgc tcggccaggt cgtcgagctc aacaagcggt tcatcgtcgg gtacgacgcg 1080
tacaaggagg acccgcgcat caagaacctc gagcaccgcg tgcgcgagta caacacgctc 1140
ctgcgctaca tgggcctcaa ggaccaccag gtcgaccgca tcggccggcc ccgctggcgc 1200
tcgttcgccc tcctgtgcta ccgcctcggc ctcctcggcg tgtggggcgt cctcgcgctc 1260
cccggcgtcg tcctcaactc gcccatcttc ctcgccgcca agatcatctc gcacaagaag 1320
gccaaggacg ccctcgccgc ctcgtcggtc aagatcgccg gtcgcgacgt cctcgcgacc 1380
tggaaggtcc tcgtcgcgct cggcggcgcg ccggccctgt actcggtcta cgccgtcaac 1440
gccgtcgtcc tcgcgcacaa gctcgccctg ccggccagct accggttctg ggcgccgttc 1500
gccacgttcg cgggcctgcc gctcatcggt gtcgccgcgc tcaagtttgg cgaggtcggc 1560
atggacgtct acaagtcgct ccgccctctc gtcctgtcgc tcgtccccgg caaggagccg 1620
cagctcatgc gtgtgcagcg catgcgcgag gagctcgcct cggagctcaa cctcctcgtc 1680
gacgagctcg cgcccgccct gttcgacgac ttttcggcgt cgcgcatcat cccgtcgacc 1740
acgcccgtcc ctcagcgtcg cgactcggcg cagggcaagt tcttccagca cccgctcagc 1800
tgggtcgacg agctcttctt cggcccggcg tggagctcga gcatggcgta cccgcaggac 1860
cgccacgtgc gcccgaacca gggcgagtcg ctccacgcgc cctcgacggg caacggcgcc 1920
gagagcgacc tcgacggcgc gttcaccgac ggccagggca ccggcagcgg ctacgtctcg 1980
ggctacacga ccgaggaggc gcccgactac gacgaggtca tccacatcct caaccgcgag 2040
cagggccacc cggactcgcc cgccgtcggt gcgcgcccga ccatctcgcg ccgtgcgtcg 2100
cggcagcgct cgcgttcggg cctcaacctc gccgccatga gccccgtctc gccggcgacg 2160
ccgctctcga gcgcgacggc ctcgggcgct caggccgggt cgagcggcgc cgaggcgagg 2220
cgcaggggtg cggcgccgca gcaggacgag tga 2253
<210> 117
<211> 551
<212> PRT
<213> 三角褐指藻
<400> 117
Met Ala Met Arg Ala Val Asn Gln Leu Ala Val Phe Phe Val Leu Leu
1 5 10 15
Leu Leu Thr Ile Asp Asp Tyr Leu Met Ala Glu Ala Ser Trp Arg Arg
20 25 30
Arg Ile Val Gly Asp Ile Ala Trp Ala Met Asp Val Val Pro Val Lys
35 40 45
Arg Ala Gln Asp Ser Ala Lys Arg Gly Asn Gly Met Val Ser Leu Ala
50 55 60
Thr Leu Asp Arg Asp Thr Arg Thr Ile Leu Val Ile Gly Arg Asn Thr
65 70 75 80
Phe Phe Val Ala Asp Leu Ser Pro Gly Asp Lys Ile Arg Ile Glu Gly
85 90 95
Ser Ala Val Gly Leu Lys Val Leu Asn Ile Glu Gly Asp His Lys Met
100 105 110
Thr Val Asp Gly Ile Asp Phe Pro Glu Gly Val Pro Leu Pro Asp Glu
115 120 125
Asn Val Gly Tyr Asp Ile Leu Gly Arg Val Asp Thr Lys Val Val Phe
130 135 140
Glu Lys Val Leu Asp Lys Leu Thr Ala Gly Gly Ala Val Gly Ile Phe
145 150 155 160
Pro Glu Gly Gly Ser His Asp Arg Thr Glu Leu Leu Pro Leu Lys Ala
165 170 175
Gly Val Ala Leu Ile Ala Tyr Ser Ala Phe Glu Lys Ile Gly Gln Ser
180 185 190
Val Pro Ile Val Pro Val Gly Leu Asn Tyr Phe Arg Ala His Arg Trp
195 200 205
Arg Gly Arg Ala Val Ile Glu Tyr Gly Gln Pro Ile Ser Leu Asn Pro
210 215 220
Lys Thr Met Pro Asp Tyr Glu Ala Gly Gly Leu Arg Arg Arg Asn Val
225 230 235 240
Cys Asn Gln Leu Leu Glu Asn Ile Glu Thr Ser Met Lys Ser Val Ile
245 250 255
Val Ser Thr Pro Asp Phe Glu Thr Leu Glu Leu Ile His Thr Ala Arg
260 265 270
Arg Leu Tyr Gln Arg Lys Thr Gly Pro Met Asn Ile Ser Glu Lys Gln
275 280 285
Asp Leu Ser Arg Arg Phe Ala Glu Gly Tyr Lys Arg Leu Leu Leu Met
290 295 300
Thr Asn Gly Lys Pro Pro Thr Glu Trp Leu Asp Leu Gln Ser Arg Val
305 310 315 320
Val Glu Tyr Arg Asn Glu Leu Lys His Leu Gly Leu Lys Asp Tyr Gln
325 330 335
Val Asn Ala Leu Val Gly Glu His Leu Asp Ala Thr Met Asn Val Lys
340 345 350
Glu Val Asp Gly Asp Val Val Leu Ser Phe Leu Gln Leu Pro Tyr His
355 360 365
Ile Val His Leu Leu Leu Leu Val Ala Leu Ala Ala Val Pro Val Met
370 375 380
Leu Leu Asn Leu Pro Val Gly Val Leu Ala Gly Leu Tyr Ala Glu Gln
385 390 395 400
Arg Arg Lys Arg Ala Leu Ala Lys Ser Lys Val Lys Ile His Gly Tyr
405 410 415
Asp Val Met Leu Thr Glu Lys Val Met Phe Cys Ile Val Met Val Pro
420 425 430
Leu Met Trp Met Phe Tyr Gly Phe Leu Leu Phe Phe Leu Thr Glu Leu
435 440 445
Asp Arg Pro Thr Ile Ala Leu Gly Ile Leu Ser Met Pro Leu Phe Ser
450 455 460
Tyr Thr Gly Ile Val Ala Ala Glu Ala Gly Met Val Asp Leu Met Asp
465 470 475 480
Leu Arg Pro Phe Phe Met Arg Leu Phe Pro Ser Ala Arg Arg Arg Leu
485 490 495
Ala Ala Leu Pro Glu Lys Arg Arg Ile Leu Gln Lys Asp Leu Arg Ala
500 505 510
Phe Ile Arg Ser Ile Gly Pro Gly Leu Gly Glu Ile Tyr Phe Lys Pro
515 520 525
Asp Ile Asn Trp Lys Glu Ile Ile Glu Ala Ser Lys Ser Ser Asp Gly
530 535 540
Met Asp Pro Asn Lys Pro Lys
545 550
<210> 118
<211> 1656
<212> DNA
<213> 三角褐指藻
<400> 118
atggcgatgc gagccgtcaa tcagctggca gtctttttcg tcttgctttt gttgacaatc 60
gatgactacc ttatggcgga ggcctcatgg aggcgccgta ttgttggtga tatcgcgtgg 120
gcaatggatg ttgtgccggt caaacgtgcg caggactccg ctaagcgagg aaatgggatg 180
gtttcacttg ctactttgga tagagacaca aggacgattt tggtcattgg gagaaacaca 240
ttttttgtgg ccgacttatc cccgggagac aagattcgaa tcgaaggtag tgcggttggg 300
ctaaaagtct tgaatattga aggcgaccat aaaatgactg tggatggtat tgatttccct 360
gaaggtgtcc cacttcctga cgagaatgtt ggctatgaca ttttaggacg tgtcgatacc 420
aaggtagtct tcgaaaaggt gttggataag cttacagcag gaggggctgt cggaatcttt 480
ccggaaggag gtagccatga cagaacggag cttttacctt tgaaagcagg tgttgccctc 540
attgcttatt ctgcttttga gaagattgga cagagtgtcc ccattgttcc tgttggcttg 600
aactatttta gggcccatcg ctggcgcgga cgggcggtga ttgaatatgg ccaaccaatc 660
tctctcaacc cgaagacaat gccggattat gaagccggag gactacgaag acgcaacgtg 720
tgcaaccaac tgcttgaaaa tattgagacc tcaatgaaat ctgtaatcgt ttcgacacca 780
gactttgaga ctttagaact catccatacc gcaagacgtc tatatcaaag gaagacaggc 840
ccaatgaata tctcggagaa gcaagatctg agtcgccggt ttgcagaagg ttacaagaga 900
cttcttctca tgacaaacgg caagccaccg acagaatggt tagatcttca aagccgtgtc 960
gtcgagtata gaaacgagct aaagcatttg ggattgaaag actaccaagt gaatgccttg 1020
gtcggtgaac atctcgatgc gacaatgaac gtcaaggaag tcgacggtga tgtcgtactt 1080
tctttcttgc agcttccgta tcatatagtt cacctcctgc ttctcgtcgc tctagcagcc 1140
gtcccagtta tgcttttgaa tctcccagtc ggtgtactag ctggtttgta tgccgaacag 1200
cgccgaaagc gtgcccttgc caagtcaaaa gtcaaaattc atggatacga tgtgatgcta 1260
accgagaagg tcatgttttg tattgtcatg gtgcccttga tgtggatgtt ttacgggttt 1320
cttctctttt ttctaactga attggatcgg ccgacaattg ctttggggat tctttcgatg 1380
cctctgtttt catacacagg aatcgttgct gcagaggctg ggatggtgga cttgatggat 1440
ttgcgcccat tcttcatgcg cctgttcccg tctgctcgta gacgtcttgc ggcattgcct 1500
gaaaagcgcc gaattctaca gaaagattta cgagccttta tccgctcgat tggacctggt 1560
ctaggggaaa tctactttaa acctgacata aattggaagg aaattatcga agcatcaaaa 1620
agttctgatg gaatggatcc aaacaaaccc aagtga 1656
<210> 119
<211> 743
<212> PRT
<213> 酿酒酵母
<400> 119
Met Ser Ala Pro Ala Ala Asp His Asn Ala Ala Lys Pro Ile Pro His
1 5 10 15
Val Pro Gln Ala Ser Arg Arg Tyr Lys Asn Ser Tyr Asn Gly Phe Val
20 25 30
Tyr Asn Ile His Thr Trp Leu Tyr Asp Val Ser Val Phe Leu Phe Asn
35 40 45
Ile Leu Phe Thr Ile Phe Phe Arg Glu Ile Lys Val Arg Gly Ala Tyr
50 55 60
Asn Val Pro Glu Val Gly Val Pro Thr Ile Leu Val Cys Ala Pro His
65 70 75 80
Ala Asn Gln Phe Ile Asp Pro Ala Leu Val Met Ser Gln Thr Arg Leu
85 90 95
Leu Lys Thr Ser Ala Gly Lys Ser Arg Ser Arg Met Pro Cys Phe Val
100 105 110
Thr Ala Glu Ser Ser Phe Lys Lys Arg Phe Ile Ser Phe Phe Gly His
115 120 125
Ala Met Gly Gly Ile Pro Val Pro Arg Ile Gln Asp Asn Leu Lys Pro
130 135 140
Val Asp Glu Asn Leu Glu Ile Tyr Ala Pro Asp Leu Lys Asn His Pro
145 150 155 160
Glu Ile Ile Lys Gly Arg Ser Lys Asn Pro Gln Thr Thr Pro Val Asn
165 170 175
Phe Thr Lys Arg Phe Ser Ala Lys Ser Leu Leu Gly Leu Pro Asp Tyr
180 185 190
Leu Ser Asn Ala Gln Ile Lys Glu Ile Pro Asp Asp Glu Thr Ile Ile
195 200 205
Leu Ser Ser Pro Phe Arg Thr Ser Lys Ser Lys Val Val Glu Leu Leu
210 215 220
Thr Asn Gly Thr Asn Phe Lys Tyr Ala Glu Lys Ile Asp Asn Thr Glu
225 230 235 240
Thr Phe Gln Ser Val Phe Asp His Leu His Thr Lys Gly Cys Val Gly
245 250 255
Ile Phe Pro Glu Gly Gly Ser His Asp Arg Pro Ser Leu Leu Pro Ile
260 265 270
Lys Ala Gly Val Ala Ile Met Ala Leu Gly Ala Val Ala Ala Asp Pro
275 280 285
Thr Met Lys Val Ala Val Val Pro Cys Gly Leu His Tyr Phe His Arg
290 295 300
Asn Lys Phe Arg Ser Arg Ala Val Leu Glu Tyr Gly Glu Pro Ile Val
305 310 315 320
Val Asp Gly Lys Tyr Gly Glu Met Tyr Lys Asp Ser Pro Arg Glu Thr
325 330 335
Val Ser Lys Leu Leu Lys Lys Ile Thr Asn Ser Leu Phe Ser Val Thr
340 345 350
Glu Asn Ala Pro Asp Tyr Asp Thr Leu Met Val Ile Gln Ala Ala Arg
355 360 365
Arg Leu Tyr Gln Pro Val Lys Val Arg Leu Pro Leu Pro Ala Ile Val
370 375 380
Glu Ile Asn Arg Arg Leu Leu Phe Gly Tyr Ser Lys Phe Lys Asp Asp
385 390 395 400
Pro Arg Ile Ile His Leu Lys Lys Leu Val Tyr Asp Tyr Asn Arg Lys
405 410 415
Leu Asp Ser Val Gly Leu Lys Asp His Gln Val Met Gln Leu Lys Thr
420 425 430
Thr Lys Leu Glu Ala Leu Arg Cys Phe Val Thr Leu Ile Val Arg Leu
435 440 445
Ile Lys Phe Ser Val Phe Ala Ile Leu Ser Leu Pro Gly Ser Ile Leu
450 455 460
Phe Thr Pro Ile Phe Ile Ile Cys Arg Val Tyr Ser Glu Lys Lys Ala
465 470 475 480
Lys Glu Gly Leu Lys Lys Ser Leu Val Lys Ile Lys Gly Thr Asp Leu
485 490 495
Leu Ala Thr Trp Lys Leu Ile Val Ala Leu Ile Leu Ala Pro Ile Leu
500 505 510
Tyr Val Thr Tyr Ser Ile Leu Leu Ile Ile Leu Ala Arg Lys Gln His
515 520 525
Tyr Cys Arg Ile Trp Val Pro Ser Asn Asn Ala Phe Ile Gln Phe Val
530 535 540
Tyr Phe Tyr Ala Leu Leu Val Phe Thr Thr Tyr Ser Ser Leu Lys Thr
545 550 555 560
Gly Glu Ile Gly Val Asp Leu Phe Lys Ser Leu Arg Pro Leu Phe Val
565 570 575
Ser Ile Val Tyr Pro Gly Lys Lys Ile Glu Glu Ile Gln Thr Thr Arg
580 585 590
Lys Asn Leu Ser Leu Glu Leu Thr Ala Val Cys Asn Asp Leu Gly Pro
595 600 605
Leu Val Phe Pro Asp Tyr Asp Lys Leu Ala Thr Glu Ile Phe Ser Lys
610 615 620
Arg Asp Gly Tyr Asp Val Ser Ser Asp Ala Glu Ser Ser Ile Ser Arg
625 630 635 640
Met Ser Val Gln Ser Arg Ser Arg Ser Ser Ser Ile His Ser Ile Gly
645 650 655
Ser Leu Ala Ser Asn Ala Leu Ser Arg Val Asn Ser Arg Gly Ser Leu
660 665 670
Thr Asp Ile Pro Ile Phe Ser Asp Ala Lys Gln Gly Gln Trp Lys Ser
675 680 685
Glu Gly Glu Thr Ser Glu Asp Glu Asp Glu Phe Asp Glu Lys Asn Pro
690 695 700
Ala Ile Val Gln Thr Ala Arg Ser Ser Asp Leu Asn Lys Glu Asn Ser
705 710 715 720
Arg Asn Thr Asn Ile Ser Ser Lys Ile Ala Ser Leu Val Arg Gln Lys
725 730 735
Arg Glu His Glu Lys Lys Glu
740
<210> 120
<211> 2232
<212> DNA
<213> 酿酒酵母
<400> 120
atgtctgctc ccgctgccga tcataacgct gccaaaccta ttcctcatgt acctcaagcg 60
tcccgacggt acaaaaattc atacaatgga ttcgtataca atatacatac atggctgtat 120
gatgtgtctg tatttctgtt taatattttg ttcactattt tcttcagaga aattaaggta 180
cgtggtgcat ataacgttcc cgaagttggg gtgccaacca tccttgtgtg tgcccctcat 240
gcaaatcagt tcatcgaccc ggctttggta atgtcgcaaa cccgtttgct gaagacatca 300
gcgggaaagt cccgatccag aatgccttgt tttgttactg ctgagtcgag ttttaagaaa 360
agatttatct ctttctttgg tcacgcaatg ggcggtattc ccgtgcctag aattcaggac 420
aacttgaagc cagtggatga gaatcttgag atttacgctc cggacttgaa gaaccacccg 480
gaaatcatca agggccgctc caagaaccca cagactacac cagtgaactt tacgaaaagg 540
ttttctgcca agtccttgct tggattgccc gactacttaa gtaatgctca aatcaaggaa 600
atcccggatg atgaaacgat aatcttgtcc tctccattca gaacatcgaa atcaaaagtg 660
gtggagctct tgactaatgg tactaatttt aaatatgcag agaaaatcga caatacggaa 720
actttccaga gtgtttttga tcacttgcat acgaagggct gtgtaggtat tttccccgag 780
ggtggttctc atgaccgtcc ttcgttacta cccatcaagg caggtgttgc cattatggct 840
ctgggcgcag tagccgctga tcctaccatg aaagttgctg ttgtaccctg tggtttgcat 900
tatttccaca gaaataaatt cagatctaga gctgttttag aatacggcga acctatagtg 960
gtggatggga aatatggcga aatgtataag gactccccac gtgagaccgt ttccaaacta 1020
ctaaaaaaga tcaccaattc tttgttttct gttaccgaaa atgctccaga ttacgatact 1080
ttgatggtca ttcaggctgc cagaagacta tatcaaccgg taaaagtcag gctacctttg 1140
cctgccattg tagaaatcaa cagaaggtta cttttcggtt attccaagtt taaagatgat 1200
ccaagaatta ttcacttaaa aaaactggta tatgactaca acaggaaatt agattcagtg 1260
ggtttaaaag accatcaggt gatgcaatta aaaactacca aattagaagc attgaggtgc 1320
tttgtaactt tgatcgttcg attgattaaa ttttctgtct ttgctatact atcgttaccg 1380
ggttctattc tcttcactcc aattttcatt atttgtcgcg tatactcaga aaagaaggcc 1440
aaagagggtt taaagaaatc attggttaaa attaagggta ccgatttgtt ggccacatgg 1500
aaacttatcg tggcgttaat attggcacca attttatacg ttacttactc gatcttgttg 1560
attattttgg caagaaaaca acactattgt cgcatctggg ttccttccaa taacgcattc 1620
atacaatttg tctattttta tgcgttattg gttttcacca cgtattcctc tttaaagacc 1680
ggtgaaatcg gtgttgacct tttcaaatct ttaagaccac tttttgtttc tattgtttac 1740
cccggtaaga agatcgaaga aatccaaaca acaagaaaga atttaagtct agagttgact 1800
gctgtttgta acgatttagg acctttggtt ttccctgatt acgataaatt agcgactgag 1860
atattctcta agagagacgg ttatgatgtc tcttctgatg cagagtcttc tataagtcgt 1920
atgagtgtac aatctagaag ccgctcttct tctatacatt ctattggctc gctagcttct 1980
aacgccctat caagagtgaa ttcaagaggc tcgttgaccg atattccaat tttttctgat 2040
gcaaagcaag gtcaatggaa aagtgaaggt gaaactagtg aggatgagga tgaatttgat 2100
gagaaaaatc ctgccatagt acaaaccgca cgaagttctg atctaaataa ggaaaacagt 2160
cgcaacacaa atatatcttc gaagattgct tcgctggtaa gacagaaaag agaacacgaa 2220
aagaaagaat ga 2232
<210> 121
<211> 745
<212> PRT
<213> 达氏诺莫维酵母
<400> 121
Met Thr Met Lys Glu Lys Pro Val Glu Thr Ile Asp Val Asn Pro Lys
1 5 10 15
Pro Lys Ser Arg Ala His Ile Pro Lys Ile Ser Arg Gln Tyr Lys Asn
20 25 30
Asp Tyr Thr Gly Leu Thr Tyr Asn Leu Lys Ser Phe Thr Tyr Asp Ile
35 40 45
Val Val Phe Leu Phe Asn Ile Leu Phe Thr Ile Phe Phe Arg Glu Ile
50 55 60
Lys Val Arg Gly Gly Tyr Asn Ile Pro Pro Asn Gly Thr Pro Thr Ile
65 70 75 80
Leu Val Cys Ala Pro His Ala Asn Gln Phe Ile Asp Pro Ser Leu Val
85 90 95
Met Thr Thr Thr Arg Lys Leu Ala Ala Thr His Gly Ser Ser Arg Gly
100 105 110
Arg Gln Ala Cys Phe Val Thr Ala Ala Ser Ser Leu Lys Leu Lys Leu
115 120 125
Val Gly Phe Phe Gly Arg Cys Met Gly Ser Val Pro Val Glu Arg Ala
130 135 140
Gln Asp Asn Leu Lys Pro Val Ser Ser Asn Ile Glu Ile Tyr Ala Pro
145 150 155 160
Asp Leu Ile Asn Asn Ser Thr Leu Ile Lys Gly Arg Cys Arg Thr Gly
165 170 175
Glu Asn Ile Ser Pro Gln Phe Thr Lys Arg Phe Asn Ala Lys Gly Leu
180 185 190
Leu Gly Leu Pro Asn Tyr Leu Ser Asn Ala Gln Ile Ala Lys Val Val
195 200 205
Asp Asp Glu Thr Ile Ile Leu Ser Ser Pro Phe Lys Ser Ser Asn Pro
210 215 220
Lys Val Arg Glu Tyr Leu Glu Glu Gly Thr Thr Phe Lys Tyr Ala Lys
225 230 235 240
Pro Ile Asp Asn Thr Gln Val Phe Gln Asn Val Phe Asp His Leu His
245 250 255
Thr Lys Gly Cys Val Gly Ile Phe Pro Glu Gly Gly Ser His Asp Arg
260 265 270
Pro Ser Leu Leu Pro Ile Lys Ala Gly Val Ala Ile Met Ala Leu Gly
275 280 285
Ala Val Ala Ala Asp Pro Ser Met Thr Val Ser Val Val Pro Val Gly
290 295 300
Leu His Tyr Phe His Arg Asp Lys Phe Arg Ser Arg Ala Val Leu Glu
305 310 315 320
Tyr Gly Glu Pro Ile Leu Val Asn Gly Glu Met Gly Lys Gln Tyr Ala
325 330 335
Leu Asn Ser Arg Glu Thr Val Ser Lys Leu Leu Thr Lys Ile Thr Asp
340 345 350
Ala Leu Phe Ser Val Thr Glu Asn Ala Pro Asp Phe Asp Thr Leu Met
355 360 365
Thr Ile Gln Ala Ala Arg Arg Leu Tyr Gln Arg Ser Lys Leu Thr Leu
370 375 380
Ser Leu Pro Val Ile Val Glu Ile Asn Arg Arg Leu Leu Val Gly Tyr
385 390 395 400
Ser Lys Phe Lys Asp Asp Glu Arg Ile Ile Asn Leu Lys Lys Met Val
405 410 415
His Glu Tyr Asn Asp Lys Leu Phe Ala Met Gly Leu Lys Asp His Gln
420 425 430
Val Met Ser Leu His Thr Gly Pro Leu Glu Thr Ile Arg Cys Leu Phe
435 440 445
Tyr Ile Val Ser Arg Val Ala Arg Leu Ser Val Phe Phe Ala Leu Ser
450 455 460
Leu Pro Gly Ser Ile Leu Phe Thr Pro Ile Phe Val Gly Cys Ser Ile
465 470 475 480
Tyr Ser Lys Lys Lys Ala Arg Glu Gly Leu Lys Lys Ser Leu Val Lys
485 490 495
Ile Lys Gly Thr Asp Leu Leu Ala Thr Trp Lys Leu Ile Val Ala Leu
500 505 510
Ile Met Ala Pro Ile Leu Tyr Val Thr Tyr Ser Leu Met Leu Val Ser
515 520 525
Ile Ala Ser Lys Asn Glu Ile Ser Ile Trp Val Pro Ser Ser Ser Pro
530 535 540
Ile Ile Gln Phe Phe Tyr Phe Tyr Ala Ile Leu Val Phe Ile Ser Tyr
545 550 555 560
Ser Ser Leu Lys Thr Gly Glu Ile Gly Met Asp Leu Phe Lys Ser Leu
565 570 575
Arg Pro Leu Phe Ile Thr Leu Phe Tyr Pro Lys Gln Lys Ile Glu Glu
580 585 590
Ile Gln Thr Thr Arg Lys Lys Leu Ser Leu Glu Ile Thr Thr Ile Cys
595 600 605
Asn Glu Leu Gly Pro His Val Phe Lys Asp Phe Asp Gln Phe Ala Thr
610 615 620
Thr Asn Lys Leu Thr Asp Glu Ser Asp Ser Lys Leu Thr Val Arg Gly
625 630 635 640
Arg His Gln Glu Gln Thr Pro Asp Phe Leu Lys Ile Gln His Asp Ser
645 650 655
Ile Arg Gly Arg Ser Ser Asp Arg Asp Val Gly Ser Ser Arg Ser Ser
660 665 670
Ser Val Gly Ser Val Val Ser Arg Ile Ser Ser Ala Leu Ser Arg Val
675 680 685
Asn Ser Arg Gly Ser Leu Ser Asn Val Pro Ile Leu Ser Glu Gly Arg
690 695 700
Ser Asn Tyr Arg Tyr Val Tyr Asp Ser Ser Ser Ser Asp Ser Asp Asn
705 710 715 720
Glu Asp Ala Thr Gly Asn Ser Lys Ile Thr Ser Leu Ile Arg Glu Lys
725 730 735
Trp Glu Ala Ser His Ala Lys Gly Glu
740 745
<210> 122
<211> 2238
<212> DNA
<213> 达氏诺莫维酵母
<400> 122
atgactatga aggagaaacc agtggaaacc atcgatgtta atcccaaacc taagtcaagg 60
gctcatattc ccaagatatc acgacagtac aagaatgatt ataccggtct aacatacaat 120
ttaaaatctt ttacttacga cattgtcgtt ttccttttta atatcctttt tacaattttc 180
tttagggaga ttaaagtccg tggtggttat aacataccac cgaacggtac accaacaatt 240
ttagtctgtg ctcctcatgc gaatcaattc attgatcctt ctctagtcat gaccactacg 300
aggaaactcg ctgctactca tggtagttca agaggtagac aagcttgctt tgtaactgcc 360
gcctccagtt tgaaattgaa attagtaggg tttttcggtc gttgtatggg gagtgtcccc 420
gtagagagag ctcaggataa tttaaaacct gtctcctcaa atattgaaat ttatgctcct 480
gacttaatta acaattcaac tttaattaaa ggtagatgtc gtacgggaga aaacatctca 540
cctcaattta caaagaggtt caatgcaaaa ggtttacttg gattaccaaa ttatttaagc 600
aatgctcaaa ttgcaaaagt ggttgatgat gaaactataa tcttatcatc tcctttcaaa 660
tcttccaatc cgaaagtgag agaatattta gaggaaggaa ccactttcaa atatgctaaa 720
ccaattgata atactcaagt gttccaaaac gtttttgatc atttacatac taagggttgc 780
gttggtattt ttccagaagg tgggtcacat gacagacctt ctttattacc aataaaagca 840
ggtgtcgcga ttatggcatt aggtgctgtt gccgcagatc catcaatgac agtttctgtc 900
gtaccagtcg gattacatta tttccataga gataaattta gatcaagagc tgtattggaa 960
tatggtgaac caattttagt taatggagaa atgggtaagc aatatgcatt gaattcaagg 1020
gaaactgttt ctaaattatt gacaaagatt actgatgcgc tattttctgt tacagagaat 1080
gctcctgatt tcgacacttt aatgactatt caagctgccc gtagattata tcaacgttcc 1140
aaattgacac taagtttacc tgtcattgtc gagattaata gaagattact tgttggttat 1200
tctaagttta aagatgacga aagaattatc aatttgaaaa aaatggttca tgaatataat 1260
gataaattat ttgcaatggg attaaaagat catcaagtta tgtcattaca tactggtcca 1320
ttagaaacaa taagatgttt attttatatt gtttcaagag tagcaagatt atctgtgttt 1380
tttgctctat cattaccggg atctatatta tttacaccaa ttttcgttgg ttgtagtatt 1440
tattccaaga aaaaagcaag agaaggttta aagaaatcct tagtaaaaat taaaggcaca 1500
gatttattag ctacttggaa acttatagtt gcattaatta tggccccaat tctttatgtc 1560
acgtattcgt taatgttagt ttccattgca tccaaaaatg aaatatcaat ttgggttccg 1620
tcatcaagtc caatcatcca attcttttat ttttatgcca tactagtctt catttcatat 1680
tcaagtttga aaactggtga aatcggaatg gatcttttca aatcattacg ccctttattt 1740
attacattat tctatcctaa gcaaaaaatt gaagagattc aaacaacgag aaagaaattg 1800
agtttagaaa ttacaactat ttgtaatgaa ttaggtccac atgtattcaa agattttgat 1860
caatttgcaa ctaccaataa attaactgat gaatctgata gtaaattgac agttagaggt 1920
cgtcaccaag aacaaactcc tgatttccta aagattcaac atgattcaat tcgtggacgt 1980
agtagtgata gagacgttgg ttctagtaga tcttcttctg tagggtctgt cgtttcaaga 2040
atatcaagtg cgttgtctag agtcaattct agaggttcat tgtctaatgt tccaatttta 2100
tcagagggga ggtcaaatta ccgatatgtt tatgattcct cttcaagtga tagtgataat 2160
gaagatgcta cgggaaattc gaagatcact tctttaatta gagaaaaatg ggaagcttct 2220
catgcaaagg gtgaataa 2238
<210> 123
<211> 722
<212> PRT
<213> 德尔布有孢圆酵母
<400> 123
Met Ala Glu Asp Ser Asn Pro Ser Ser Lys Lys Gln Ile Lys Ala His
1 5 10 15
Val Pro Asn Ala Lys Ile Glu Tyr Asp Asn Lys Tyr Asn Gly Tyr Gly
20 25 30
Tyr Ser Ile Gln Ala Trp Leu Phe Asp Cys Val Leu Phe Leu Leu Asn
35 40 45
Ile Val Phe Thr Ile Phe Phe Arg Glu Ile Lys Val Arg Gly Gly His
50 55 60
Asn Val Pro Pro Ile Gly Thr Ala Thr Met Leu Val Cys Ala Pro His
65 70 75 80
Ala Asn Gln Phe Ile Asp Pro Ser Leu Val Met Val Thr Thr Arg Lys
85 90 95
Leu Ala Lys Glu Ser Arg Asn Arg Ser Arg Gln Val Cys Phe Val Thr
100 105 110
Ala Glu Ser Ser Leu Lys Lys Lys Phe Val Ser Leu Phe Gly Ile Cys
115 120 125
Thr Gly Ala Ile Pro Val Pro Arg Ala Gln Asp Asn Leu Lys Pro Val
130 135 140
Asp Ser Asp Val Lys Ile Tyr Cys Pro Asp Leu Glu Asn Asn Ala Thr
145 150 155 160
Met Val Lys Gly Arg Leu Asp Ser Gly Gly Ser Pro Lys Phe Thr Lys
165 170 175
Arg Phe Thr Ala Lys Ser Leu Ile Gly Leu Pro Asn Tyr Leu Gly Asn
180 185 190
Ala Gln Ile Ala Glu Val Lys Asp Asp Glu Thr Ile Ile Leu Lys Ala
195 200 205
Pro Phe Lys Phe Lys Thr Asn Glu Lys Ile Arg Ser Leu Leu Thr Glu
210 215 220
Gly Thr Thr Phe Lys Tyr Ala Pro His Ile Asp Asn Ser Lys Val Phe
225 230 235 240
Gln Asn Val Phe Gly His Leu His Thr Lys Gly Thr Val Gly Ile Phe
245 250 255
Pro Glu Gly Gly Ser His Asp Arg Pro Ser Leu Leu Pro Ile Lys Ala
260 265 270
Gly Val Ala Ile Met Ala Leu Gly Ala Thr Ala Ala Asp Pro Ser Met
275 280 285
Lys Val His Val Val Pro Cys Gly Leu His Tyr Phe His Arg Asp Lys
290 295 300
Phe Arg Ser Arg Ala Val Leu Glu Tyr Gly Glu Pro Ile Ile Val Asp
305 310 315 320
Gly Glu Met Gly Lys Arg Tyr Ala Lys Asn Pro Arg Glu Thr Val Gln
325 330 335
Glu Leu Leu Lys Val Ile Thr Ser Ser Leu Tyr Ala Val Thr Glu Asn
340 345 350
Ala Pro Asp Phe Asp Val Leu Met Thr Ile Gln Ala Ala Arg Arg Leu
355 360 365
Tyr Gln Pro Thr Arg Gly Lys Ile Pro Leu Pro Thr Val Val Asp Ile
370 375 380
Asn Arg Arg Leu Leu Leu Gly Tyr Ser Thr Tyr Lys Asp Asp Pro Arg
385 390 395 400
Ile Ile His Leu Gln Lys Met Val Asn Arg Tyr Asn Asn Ala Leu Tyr
405 410 415
Ser Leu Gly Leu Lys Asp His Gln Val Ala Glu Leu Lys Thr Lys Lys
420 425 430
Arg Glu Val Leu Arg Ser Leu Phe Val Leu Val Glu Arg Met Ile Arg
435 440 445
Leu Leu Ile Phe Phe Cys Leu Ser Leu Pro Gly Ser Ile Leu Phe Thr
450 455 460
Pro Ile Phe Ile Ser Cys Ser Ile Tyr Ala Lys Lys Lys Ala Lys Glu
465 470 475 480
Gly Leu Ser Lys Ser Leu Val Lys Ile Lys Gly Thr Asp Leu Ile Ala
485 490 495
Thr Trp Lys Leu Met Phe Ala Leu Val Phe Ala Pro Leu Leu Tyr Val
500 505 510
Thr Tyr Ser Leu Ile Leu Val Tyr Phe His Ala Arg His Tyr Glu Trp
515 520 525
Ile Ser Lys Ile Tyr Val Pro Phe Asp Asn Lys Phe Leu Gln Phe Cys
530 535 540
Tyr Tyr Tyr Gly Leu Leu Val Ala Ala Thr Tyr Ser Ser Leu Lys Thr
545 550 555 560
Gly Glu Ile Gly Met Asp Leu Phe Lys Ser Leu Pro Pro Leu Phe Ile
565 570 575
Thr Leu Ile Tyr Pro Gly Arg Lys Ile Gln Gln Leu Lys Ser Met Arg
580 585 590
Glu Lys Leu Ser Gln Glu Ile Thr Ala Val Ile Asn Glu Leu Gly Pro
595 600 605
Lys Leu Phe Pro Asn Phe Asp Lys Ile Ile Lys Ala His Phe Asp Ser
610 615 620
His Val Glu Glu Val Ile Ser Glu Ala Glu Glu Glu Leu Thr Asp Asp
625 630 635 640
Ser Ala Ala Val Phe Ser Asn Ser Arg Ser Arg Ser Gly Ser Val His
645 650 655
Ser Ala Thr Ser Thr Ser Ser Asn Ala Leu Ser Lys Val Asn Ser Arg
660 665 670
Gly Ser Leu Thr Asp Val Pro Ile Phe Ala Glu Gly Lys His Thr Leu
675 680 685
His His Ser Asp Asp Glu Ile Glu Leu Ser Pro Glu Asp Lys Glu Asn
690 695 700
Asp Ser Lys Ile Thr Ala Met Ile Arg Glu Lys Trp Glu Arg Glu Lys
705 710 715 720
Lys Asp
<210> 124
<211> 2169
<212> DNA
<213> 德尔布有孢圆酵母
<400> 124
atggcggaag attcaaaccc cagctcaaag aaacaaataa aggctcatgt gcctaatgct 60
aagatcgaat acgataacaa gtataatggg tatggatatt ctattcaagc atggttattc 120
gattgtgtat tgttcttgtt gaacattgtt ttcaccatat tctttcgaga aattaaagtt 180
cgtggtggtc acaacgtccc acccatcgga acagcgacaa tgttggtgtg tgcccctcat 240
gcaaatcaat tcatcgaccc ttctctagtg atggtaacca ctaggaaact tgccaaagaa 300
tctagaaata gatctagaca ggtttgcttt gtaactgcag aatctagtct caagaagaaa 360
ttcgtgtctc tctttggtat atgtacaggc gctatcccag tgccaagggc ccaggacaac 420
ttgaaaccag ttgattctga tgttaagata tactgtccgg atttagaaaa taatgctact 480
atggtgaagg gccgtttgga ctctggaggt tctccaaaat ttaccaagag attcacagcc 540
aaatctctaa ttggtttgcc caactatctc gggaatgccc aaattgcaga ggttaaggat 600
gatgaaacga ttatacttaa ggctccgttc aagtttaaga ccaacgaaaa gattagatca 660
ttattgaccg agggaacaac ttttaaatac gctccacaca tcgacaactc caaagttttt 720
caaaacgttt ttggccattt acatacgaaa ggtactgttg ggatctttcc agagggtggg 780
tcccacgata gaccttcatt gttaccgatt aaggctggtg tcgctattat ggctctcggt 840
gccacggcag cagatccaag catgaaagtt catgttgtac catgtgggct gcactacttt 900
cacagggaca aattcagatc aagagccgtc ttggagtatg gcgagcctat cattgtcgat 960
ggagaaatgg gaaaaagata tgcgaaaaat cctagagaaa ctgtacaaga attattgaaa 1020
gtcattacta gttccctgta tgctgtgacc gaaaatgcgc cggatttcga cgttttaatg 1080
actattcagg cggcaagaag actctatcaa ccaacaagag ggaagattcc tttacctaca 1140
gtggtcgata tcaacagaag attattgctc ggctactcaa cttataagga tgatcctcgt 1200
atcattcatt tgcaaaaaat ggtcaacagg tataacaacg ccttgtactc attgggactc 1260
aaggatcatc aagtcgcaga gttgaaaacg aagaagagag aagtacttag aagtttgttc 1320
gttttggttg aaagaatgat aaggcttctc attttctttt gtttgtctct accgggttcc 1380
atcctattca ctccaatttt tattagttgc agcatttacg ccaagaagaa ggcaaaggaa 1440
ggcttgtcga agtctttggt aaagataaaa ggtactgatt tgattgccac atggaagcta 1500
atgtttgcac tcgtatttgc ccctcttctt tatgtcactt attcgttgat tttggtatat 1560
tttcatgcac gtcattacga gtggatttcc aagatttatg ttccatttga caacaaattt 1620
ctgcagttct gctactatta tggtcttctt gtggctgcca catattcaag tttgaaaacc 1680
ggagaaatag gaatggattt gttcaagtca ttaccacctc tattcatcac tctcatatac 1740
ccaggcagga agattcaaca gttgaaaagc atgcgtgaaa aactgagcca agaaataaca 1800
gctgtgatta acgagcttgg tcctaaactt ttccccaatt ttgacaagat tatcaaagca 1860
catttcgata gccatgttga agaagtcata tctgaagctg aagaagagct aactgatgat 1920
agcgcggcgg tgttcagcaa ctcgcgcagt cggtcaggct ctgttcattc tgcaacttcc 1980
acgagctcaa atgctttgtc aaaggttaac tcaagaggtt ctttgaccga tgtgcctatc 2040
tttgccgagg gaaaacatac tttgcatcac agtgatgatg agatagagtt gtcacctgaa 2100
gacaaagaga acgattctaa gatcactgct atgattagag agaaatggga gcgtgagaag 2160
aaagattaa 2169
<210> 125
<211> 733
<212> PRT
<213> 卡氏诺莫维酵母
<400> 125
Met Pro Pro Lys Ser Asn Ala His Val Pro Glu Val Ser Arg Thr Tyr
1 5 10 15
Lys Asn Asn Phe Asn Gly Leu Val Tyr Asn Ile His Thr Trp Thr Tyr
20 25 30
Asp Cys Ile Ile Phe Leu Phe Asn Ile Ile Phe Thr Ile Phe Phe Arg
35 40 45
Glu Ile Lys Val Arg Gly Gly Tyr Asn Val Pro Pro Ala Gly Thr Pro
50 55 60
Thr Ile Leu Val Cys Ala Pro His Ala Asn Gln Phe Ile Asp Pro Thr
65 70 75 80
Leu Val Met Val Thr Thr Arg Lys Leu Gly Met Tyr Gly Ala Thr Ser
85 90 95
Val Ser Arg Ser Arg Gln Ala Cys Phe Val Thr Ala Ala Ser Ser Leu
100 105 110
Asn Met Lys Leu Val Gly Phe Phe Gly Arg Arg Met Gly Gly Ile Pro
115 120 125
Val Ala Arg Ala Gln Asp Tyr Leu Lys Pro Val Asp Asp Asn Leu Glu
130 135 140
Ile Tyr Ala Pro Asp Leu Glu Asn Asn Pro Lys Leu Ile Lys Gly Arg
145 150 155 160
Cys Lys Asp Ser Lys Ser Pro Glu Phe Thr Lys Arg Phe Thr Ala Lys
165 170 175
Ser Leu Leu Gly Leu Pro Asn Tyr Leu Ser Asn Ala Gln Ile Ala Gln
180 185 190
Ile Gln Asp Asp Glu Thr Ile Ile Leu Ser Ser Pro Phe Lys Ile Ser
195 200 205
Asp Pro Arg Val Arg Lys Leu Leu Asn Asn Gly Thr Thr Phe Lys Tyr
210 215 220
Ala Asn Lys Val Asp Asn Ser Lys Val Phe Gln Ser Val Phe Asp His
225 230 235 240
Leu His Thr Lys Gly Cys Val Gly Ile Phe Pro Glu Gly Gly Ser His
245 250 255
Asp Arg Pro Ser Leu Leu Pro Ile Lys Ala Gly Val Ala Ile Met Ala
260 265 270
Leu Gly Ala Ala Ala Ala Asp Ala Asn Ile Lys Val His Val Val Pro
275 280 285
Val Gly Leu His Tyr Phe His Arg Asp Lys Phe Arg Ser Arg Ala Val
290 295 300
Leu Glu Tyr Gly Glu Pro Ile Val Val Asp Gly Thr Met Gly Lys Glu
305 310 315 320
Tyr Ala Gln Ala Pro Arg Glu Thr Val Ser Lys Leu Leu Ser Arg Ile
325 330 335
Thr Asp Ala Leu Phe Ser Val Thr Glu Asn Ala Pro Asp Tyr Asp Thr
340 345 350
Leu Met Thr Ile Gln Ala Ala Arg Arg Leu Tyr Gln Pro Ser Glu Lys
355 360 365
Arg Leu Ser Leu Pro Val Val Val Glu Ile Asn Arg Arg Leu Leu Val
370 375 380
Gly Tyr Ser Lys Phe Lys Ser Asp Glu Arg Val Ile His Leu Lys Lys
385 390 395 400
Met Val Gln Glu Tyr Asn Glu Lys Leu Tyr Ser Met Gly Leu Lys Asp
405 410 415
His Gln Val Arg Glu Leu Glu Ser His Thr Val Gln Asn Thr Ile Arg
420 425 430
Thr Leu Val Thr Leu Val Thr Arg Val Ser Arg Leu Leu Leu Phe Phe
435 440 445
Met Leu Ala Leu Pro Gly Ser Ile Leu Phe Thr Pro Ile Phe Ile Gly
450 455 460
Ser Ser Ile Tyr Ser Lys Lys Lys Ala Arg Glu Gly Leu Lys Lys Ser
465 470 475 480
Leu Val Lys Ile Lys Gly Thr Asp Leu Leu Ala Thr Trp Lys Leu Ile
485 490 495
Leu Ala Leu Val Met Ala Pro Ile Ser Tyr Val Thr Tyr Ser Leu Ile
500 505 510
Leu Ile Ser Leu His Ser Arg Lys Asn Gly Trp Val Gln Trp Ile Trp
515 520 525
Val Pro Ser Glu Asn Val Phe Ile Gln Phe Pro Tyr Phe Tyr Met Gln
530 535 540
Leu Val Leu Thr Thr Tyr Gly Ser Leu Lys Thr Gly Glu Ile Gly Met
545 550 555 560
Asp Leu Phe Lys Ser Leu Arg Pro Leu Val Val Thr Leu Met Tyr Pro
565 570 575
Gly Lys Lys Ile Arg Glu Ile Gln Ser Ile Arg Glu Gln Leu Ser Glu
580 585 590
Glu Ile Thr Ser Val Cys Asn Glu Leu Gly Pro Ser Val Phe Lys Asp
595 600 605
Phe Asp Gln Phe Ala Ile Asn Asn Glu Ile Glu Ser Glu Arg Gly Arg
610 615 620
Gly Arg Tyr Glu Lys Glu Lys Thr Pro Asp Tyr Leu Lys Ile Gln Arg
625 630 635 640
Asp Pro Ser Arg Ser Arg Ser Arg Gly Ala Arg Ser Arg Ser Ser Ser
645 650 655
Ile Ser Ser Phe Thr Ser Arg Ile Ser Asn Ala Ile Ser Arg Val Asn
660 665 670
Ser Arg Gly Ser Leu Ser Asp Ile Pro Ile Leu Ser Glu Ala Arg Tyr
675 680 685
Ser Ser Asn Asn Val Ile Asn Asp Ser Asp Ser Ser Cys Ser Ser Ser
690 695 700
Asp Glu Glu Asn Ile Lys Ala Gly Ser Thr Ser Lys Ile Ser Ser Leu
705 710 715 720
Met Arg Ala Arg Trp Glu Lys Ser His Asp Lys Glu Glu
725 730
<210> 126
<211> 2202
<212> DNA
<213> 卡氏诺莫维酵母
<400> 126
atgcccccta aatcaaacgc tcacgtccca gaggtttcac gaacgtacaa gaacaacttc 60
aatgggctgg tatacaatat tcacacatgg acttacgatt gcataatatt cctcttcaat 120
attatattca ctattttctt ccgtgaaatt aaagttcgtg gaggttacaa cgtacctccc 180
gctgggaccc ccacaatact cgtctgtgct cctcatgcca atcaattcat tgaccctaca 240
ttggtaatgg tgacaaccag aaaactaggt atgtatggtg ccacctctgt ctctcgttca 300
agacaggcat gtttcgttac agctgcttca agtttgaata tgaaacttgt tggatttttt 360
ggaagaagaa tgggtgggat tcctgttgct agagcacaag attacttaaa accagtagat 420
gacaacttgg aaatctacgc tcccgacttg gaaaataatc caaaattaat aaaaggtcgt 480
tgtaaggatt ctaaatcacc tgaattcaca aagagattca ctgcaaaatc ccttttgggg 540
ttgcctaatt atttaagtaa tgcccaaatt gctcagattc aagatgatga aactatcata 600
ttaagctctc cctttaagat ttctgatcca agagtgagga aactattaaa taacggtaca 660
actttcaaat atgctaacaa ggtggataat agtaaagtat tccagagtgt ttttgatcat 720
ctgcatacaa aaggttgtgt cgggattttc ccagaaggtg gatctcacga tagaccttct 780
ttattaccta ttaaagcagg tgttgccatt atggccttag gtgctgcagc agctgatgca 840
aatataaaag tacatgttgt gcccgttggg ttgcattact tccacagaga caaatttaga 900
tcaagagctg ttctcgagta cggtgaaccc atagtcgtgg atggaaccat gggaaaagaa 960
tatgcacagg ctccacgtga aacagtttct aaattgttgt caagaattac agatgcacta 1020
ttctctgtta cagaaaatgc ccctgattat gatactttga tgactatcca ggcagctaga 1080
agattgtacc aaccttctga gaaaagatta tctttgcccg ttgtcgttga aattaataga 1140
agattactcg tgggatattc aaaatttaaa agtgatgaaa gggtcattca tttgaagaaa 1200
atggttcaag aatacaatga aaaattatac tctatggggt taaaagatca tcaagttaga 1260
gaattagaat ctcatactgt gcaaaataca attagaactt tggttacctt agtgacaaga 1320
gtttctagat tattactatt cttcatgttg gctcttcccg ggtccattct tttcactcct 1380
atcttcatcg gttctagcat ctattcaaag aagaaggcaa gagaagggtt gaaaaaatca 1440
ttagtgaaaa ttaagggaac tgatttatta gccacttgga aacttatctt agcacttgtg 1500
atggctccaa tttcatatgt tacttattcc ctcattttaa tttccctgca ttctcgtaaa 1560
aatggatggg ttcaatggat ttgggtacct agtgaaaatg ttttcatcca attcccttat 1620
ttttacatgc agttggtact taccacatat ggttctttga agactggtga aattggaatg 1680
gatcttttta aatctttacg tccccttgta gtcaccttga tgtatccagg taagaaaatc 1740
agagaaattc aatcaatccg tgaacaattg agtgaagaga tcacttcagt ttgtaacgag 1800
ttaggtcctt ccgttttcaa agactttgat caatttgcaa ttaataacga gatcgagagt 1860
gaaagaggca gaggtcgcta tgagaaagaa aagactcctg attatttgaa gattcaacgt 1920
gatcctagtc gtagccgtag tcgtggtgct agaagtcgtt catcatcgat tagttcattt 1980
acttcacgta tctctaatgc tatttctaga gtgaactcca gaggttccct ctctgatatt 2040
ccaattttat cagaagcaag atatagttcc aataatgtca tcaatgatag tgattcatcc 2100
tgttcctctt ctgatgaaga gaatataaaa gcaggttcta cttctaaaat ttcatcttta 2160
atgagagcta gatgggaaaa atcgcatgat aaggaggaat ag 2202
<210> 127
<211> 370
<212> PRT
<213> 花药黑粉菌
<400> 127
Met Thr Ser Leu Arg Asp Val Asn Pro Thr Ser Thr Gln Ala Ser Leu
1 5 10 15
Tyr Lys Asp Glu Gly Lys Asp Lys Glu Asp Val Ala Pro Gln Glu Lys
20 25 30
Tyr Thr Gln Ser Leu Arg Thr Asn Ile Lys Phe Ala Pro Leu Ala Val
35 40 45
Pro Arg His Arg Arg Leu Gln Thr Met Ala Val Leu Gly Trp Thr Thr
50 55 60
Ala Leu Pro Leu Met Leu Gly Leu Phe Phe Leu Leu Cys Ser Ile Pro
65 70 75 80
Leu Leu Trp Pro Ile Ile Val Pro Tyr Leu Phe Trp Ile His Leu Ile
85 90 95
Asp Asn Ser Pro Thr Gln Gly Gly Arg Ala Ser Lys Trp Leu Arg Gln
100 105 110
Ser Arg Phe Trp Val Trp Phe Thr Gly Tyr Tyr Pro Ile Ser Leu Val
115 120 125
Lys Thr Val Asp Leu Pro Pro Asp Arg Lys Tyr Val Phe Gly Tyr His
130 135 140
Pro His Gly Ile Ile Gly Met Gly Ala Ile Ala Asn Phe Gly Thr Asp
145 150 155 160
Ala Thr Gly Phe Ser Glu Leu Phe Pro Gly Leu Asn Pro His Leu Leu
165 170 175
Thr Leu Ala Ser Asn Phe Lys Leu Pro Ile Tyr Arg Asp Phe Leu Leu
180 185 190
Ala Leu Gly Ile Cys Ser Val Ser Met Lys Ser Cys Gln Asn Ile Leu
195 200 205
Lys Gln Gly Pro Gly Ser Ala Leu Thr Ile Val Val Gly Gly Ala Ala
210 215 220
Glu Ser Leu Ser Ala His Pro Gly Thr Ala Asn Leu Thr Leu Arg Arg
225 230 235 240
Arg Met Gly Phe Ile Lys Leu Ala Met Arg Gln Gly Ala Asp Leu Val
245 250 255
Pro Val Phe Ser Phe Gly Glu Asn Asp Ile Phe Glu Gln Met Pro Asn
260 265 270
Glu Arg Gly Thr Lys Leu Tyr Lys Met Gln Lys Lys Phe Gln Thr Ala
275 280 285
Phe Gly Phe Thr Leu Pro Ile Phe His Gly Arg Gly Ile Phe Asn Tyr
290 295 300
Asn Leu Gly Ile Leu Pro Tyr Arg His Pro Ile Val Ser Val Val Gly
305 310 315 320
Arg Pro Ile Arg Val Ser Gln Arg Asp Asn Pro Thr Lys Glu Glu Leu
325 330 335
Glu Glu Val Gln Glu Arg Tyr Ile Glu Glu Leu Lys Arg Ile Trp Asp
340 345 350
Asp Tyr Lys Asn Gln His Ala Ile Lys Arg Lys Gly Glu Leu Arg Ile
355 360 365
Ile Ala
370
<210> 128
<211> 1113
<212> DNA
<213> 花药黑粉菌
<400> 128
atgacgtcgc tgcgagacgt gaacccgacc tcgacccaag catcgttgta caaagacgag 60
ggcaaggaca aggaggatgt cgcaccgcag gagaaataca cgcagtcgct ccggaccaac 120
atcaaatttg cacctctagc tgtaccacgc catcgacgac tgcagaccat ggcagtgttg 180
ggatggacga ccgcactgcc actcatgctt ggtttgttct ttctattgtg ctcaatcccc 240
cttctatggc ccatcatcgt gccctatctc ttctggatcc acctcatcga caactcgccg 300
acgcagggag gacgagcgag caaatggctt cgccaaagtc ggttctgggt gtggttcaca 360
gggtactatc ctatcagtct cgtcaagacg gtcgatttac ctccagatcg gaaatacgtc 420
ttcggttacc acccccatgg cataattgga atgggtgcaa ttgccaattt tgggaccgac 480
gccactgggt tctctgagct cttcccagga ctcaaccctc atctcctcac acttgccagc 540
aacttcaaat tgccgatata tcgagacttc ttgcttgcgc tcggcatctg ctcagtcagt 600
atgaaatctt gccaaaacat cctcaaacag ggcccggggt ctgctttgac cattgtcgtc 660
ggaggagctg cggaatccct ttcggcgcat cctggcacag ccaacttgac actccgtcgc 720
cgaatgggct tcatcaagct ggcgatgcgt caaggcgcgg atcttgtacc cgtcttttca 780
ttcggagaga acgatatctt cgaacagatg ccgaacgaga gagggacgaa gctgtacaag 840
atgcaaaaga agtttcagac cgcttttgga ttcactctac cgatcttcca cggccgagga 900
atttttaact ataaccttgg catcttgccg taccgtcatc cgatcgtgtc ggtcgtcggt 960
cggcccatcc gcgtttcgca gcgtgacaac cctactaagg aggaactcga ggaggtgcag 1020
gaacgataca tcgaggagtt gaagagaatc tgggacgatt acaaaaatca acatgccatc 1080
aagcgaaagg gcgaacttcg tattattgcc tga 1113
<210> 129
<211> 365
<212> PRT
<213> 禾柄锈菌
<400> 129
Met Lys Asp Asp Ser Arg Ser Pro Ser Gly Ser Glu Pro Glu Gly Asp
1 5 10 15
Asn His Lys Lys Glu Lys Arg Pro Ile Trp Ala Pro Ile Arg Val Pro
20 25 30
Pro Tyr Arg Arg Ile Gln Thr Ala Ala Val Leu Leu Trp Thr Ser Gln
35 40 45
Leu Ser Leu Cys Ile Ser Leu Phe Phe Phe Leu Met Ser Tyr Pro Ile
50 55 60
Thr Trp Pro Ile Leu Leu Pro Tyr Val Ile Trp Ile Leu Val Ile Asp
65 70 75 80
Pro Ala Pro Glu Lys Gly Gly Arg Leu Asn Gln Ser Val Arg Thr Trp
85 90 95
Lys Phe Trp Asn Leu Phe Ala Ser Tyr Phe Pro Ile Ser Leu Ile Lys
100 105 110
Thr Val Asp Leu Pro Ser Asp Arg Lys Tyr Val Phe Gly Tyr His Pro
115 120 125
His Gly Ile Ile Gly Met Gly Ala Val Ala Asn Phe Gly Thr Glu Ala
130 135 140
Thr Gly Phe Ser Glu Lys Phe Pro Gly Leu Asn Pro His Leu Leu Thr
145 150 155 160
Leu Ser Thr Asn Phe Ile Ile Pro Phe Tyr Arg Asp Leu Ile Leu Ser
165 170 175
Leu Gly Ile Cys Ser Val Ser Ile Lys Ser Cys Ile Ser Ile Leu Lys
180 185 190
Ser Lys Asn Lys Arg Ser Ala Asp Val Lys Asn Asn Lys Gly Glu Gly
195 200 205
Asn Cys Leu Val Ile Val Val Gly Gly Ala Ala Glu Ser Leu Ser Ala
210 215 220
His Pro Gly Thr Ala Asp Leu Thr Leu Lys Arg Arg Leu Gly Phe Ile
225 230 235 240
Lys Leu Ala Ile Arg Glu Gly Ala Asp Leu Val Pro Val Phe Ser Phe
245 250 255
Gly Glu Asn Asp Ile Tyr Ala Gln Leu Ser Asn Ser Lys Gly Thr Ala
260 265 270
Leu Tyr Ser Leu Gln Lys Arg Phe Gln Ala Val Phe Gly Phe Thr Leu
275 280 285
Pro Val Phe His Gly Arg Gly Ile Phe Asn Tyr Ser Leu Gly Leu Leu
290 295 300
Pro Tyr Arg His Pro Ile Val Ser Val Val Gly Lys Pro Ile Arg Val
305 310 315 320
Glu Gln Asn Lys Asn Pro Gly Leu Glu Glu Ile Glu Lys Val Gln Lys
325 330 335
Glu Tyr Ile Ala Glu Leu Thr Ala Val Trp Asp Gln Tyr Lys Asp Leu
340 345 350
Tyr Ala Arg Asn Arg Lys Ser Glu Leu Thr Leu Ile Ala
355 360 365
<210> 130
<211> 1098
<212> DNA
<213> 禾柄锈菌
<400> 130
atgaaggatg actccagaag cccgtctggg tccgaacccg agggcgataa tcacaagaag 60
gagaaaaggc caatctgggc tccgattcgt gtacctcctt acaggcgcat ccaaacggcc 120
gcagtactct tatggacttc tcaactctca ctatgcattt ccttattctt tttcttaatg 180
tcttacccga tcacctggcc gatcctcctt ccatacgtta tttggatctt ggtcatagat 240
cctgctcccg agaagggtgg ccggttgaat caatctgttc ggacctggaa gttttggaat 300
ctatttgcgt cgtatttccc aatcagttta atcaaaactg ttgatttgcc cagtgaccgc 360
aaatatgtct ttggttacca ccctcatggt atcatcggaa tgggcgcggt ggccaacttt 420
ggaacggaag cgacaggatt ttcggaaaaa ttccctggtc tcaatccaca tctactcaca 480
ttgagcacga actttatcat cccattctat cgagacctga tcctcagtct tggaatctgt 540
tcggtgtcga tcaaatcatg catctcgatc ctcaaatcca aaaacaaacg ctcagctgat 600
gtcaagaaca ataagggcga aggaaattgt ttggttatcg ttgtcggtgg ggctgcggaa 660
agtttgtctg ctcatcctgg aacagccgat ctcactctaa aacgacggct gggtttcatc 720
aaactggcca ttcgagaagg agccgatctc gtccctgtgt tctcctttgg agagaatgac 780
atttacgccc aattatcaaa ctcaaaaggc acggcactct actctcttca aaaacgattt 840
caagctgtat ttggctttac cttacctgtt ttccatggcc gaggtatctt caactactct 900
ctcggcttgc ttccctatcg acacccgatt gtttcagtag ttggtaaacc tattcgagtc 960
gagcaaaata aaaaccccgg gctcgaagaa atcgaaaagg ttcagaaaga atacattgct 1020
gaacttaccg cagtatggga tcagtataaa gatttatacg ctagaaatcg gaagagtgaa 1080
ttgactttga ttgcttag 1098
<210> 131
<211> 373
<212> PRT
<213> 密粘褶菌
<400> 131
Met Asp Ala Gly Arg Ala Phe Ser Ser Ala Ser Arg Ser Leu Ser Ser
1 5 10 15
Ser Ser Leu Lys Asp Lys Leu Ser Lys Val Ser Lys Leu Ser Thr Thr
20 25 30
Pro Leu Arg Pro Val Ala Ala His Val Lys Asn Ile Asp Phe Val Pro
35 40 45
Ser Lys Ile Pro Arg Lys Arg Arg Leu Gln Met Leu Ala Val Ala Val
50 55 60
Trp Ala Leu Leu Ile Pro Ile Thr Thr Phe Leu Phe Leu Ile Leu Cys
65 70 75 80
Ser Phe Pro Pro Leu Trp Pro Phe Leu Ala Ala Tyr Leu Ile Trp Ile
85 90 95
Arg Trp Ile Asp Arg Ser Pro Glu His Gly Gly Arg Ile Ser Pro Trp
100 105 110
Phe Arg Ser Met Arg Phe Trp Arg Tyr Phe Ala Asp Tyr Tyr Pro Ala
115 120 125
Ser Phe Leu Lys Glu Cys Asp Leu Pro Pro Asp Arg Pro Tyr Val Phe
130 135 140
Gly Tyr His Pro His Gly Ile Ile Gly Met Gly Ala Met Ala Thr Phe
145 150 155 160
Ala Thr Glu Ala Thr Gly Phe Ser Glu Gln Phe Pro Gly Leu Thr Pro
165 170 175
His Leu Leu Thr Leu Ala Thr Asn Phe Thr Met Pro Ile Tyr Arg Asp
180 185 190
Ile Ile Leu Ala Leu Gly Ile Cys Ser Val Ser Lys Gln Ser Cys Ser
195 200 205
Asn Ile Leu Ser Ser Gly Pro Gly Gln Ala Ile Thr Ile Val Val Gly
210 215 220
Gly Ala Ala Glu Ser Leu Ser Ala Arg Pro Gly Thr Ala Asp Leu Thr
225 230 235 240
Leu Lys Arg Arg Leu Gly Phe Ile Lys Ile Ala Ile Gln His Gly Ala
245 250 255
Ala Leu Val Pro Val Phe Ser Phe Gly Glu Asn Asp Ile Tyr Gln Gln
260 265 270
Met Pro Asn Glu Lys Gly Thr Thr Ile Tyr Ala Leu Gln Lys Lys Phe
275 280 285
Gln Ser Val Phe Gly Phe Thr Leu Pro Leu Phe His Gly Arg Gly Met
290 295 300
Leu Asn Tyr Asn Leu Gly Leu Met Pro Tyr Arg Arg Arg Ile Val Ser
305 310 315 320
Val Ile Gly Arg Pro Ile Leu Cys Glu Lys Cys Glu Lys Pro Ser Met
325 330 335
Glu Glu Val Thr Arg Val Gln Gln Glu Tyr Ile Ala Glu Leu Leu Arg
340 345 350
Ile Trp Asp Thr Tyr Lys Asp Gln Phe Ala Arg Ser Arg Lys Arg Glu
355 360 365
Leu Ser Ile Ile Asp
370
<210> 132
<211> 1122
<212> DNA
<213> 密粘褶菌
<400> 132
atggacgctg gtcgcgcctt ctcctctgca tcccgctcgt tatcgtcctc gtccctgaag 60
gacaagctgt caaaggtctc gaagctcagc accactcctc tgcgaccggt cgctgcccat 120
gtcaagaata tcgacttcgt cccgtccaag atcccccgga aacggaggct gcagatgctc 180
gctgttgcag tatgggcgct cctgataccc atcacgacgt ttttgttcct catactatgt 240
tcttttccac cgctgtggcc atttttagcg gcgtatctta tatggataag atggatagac 300
cggagtcctg agcatggcgg gaggataagt ccgtggttcc gctcgatgag gttctggaga 360
tactttgccg actactaccc tgcatcgttc ttgaaggaat gcgacctccc cccagaccga 420
ccttacgtct tcgggtatca ccctcatggc atcattggca tgggtgccat ggccactttc 480
gccaccgaag ccactggatt cagcgaacag ttccctgggc tcactcccca cctgctcacc 540
ctagccacaa atttcaccat gcccatatac agagacatca tcctcgccct gggcatatgc 600
tccgtcagca agcagtcctg ctcgaacatc ctcagcagcg gccccgggca ggctatcaca 660
atcgtagtag gaggcgcagc agagagtctt agcgctcggc cgggcacggc cgacctcacg 720
ctcaaacgga ggcttggctt catcaagatt gctatacaac acggagcggc actggtccct 780
gtattttctt tcggcgagaa tgatatttat caacaaatgc ccaacgaaaa gggaaccaca 840
atatatgccc tacagaagaa attccagagc gtcttcggct tcacgttgcc cttgttccac 900
ggtcggggca tgctaaatta taaccttggt ttgatgccgt atcgacggcg gatcgtgtct 960
gtcatcggtc ggcccatatt atgcgagaag tgcgagaagc caagcatgga ggaggttacg 1020
cgggtgcaac aggagtacat cgcagagctg ctcagaatat gggacacgta caaagatcaa 1080
tttgctcggt cgcggaagag agaactgagt attattgatt ga 1122
<210> 133
<211> 346
<212> PRT
<213> 双倒卵形红冬孢酵母
<400> 133
Met Gly Ala Leu Asp Ala Gly Asp His Glu Gly Thr Glu His Pro Lys
1 5 10 15
Ile Lys Phe Val Pro Phe Val Val Pro Arg His Arg Arg Leu Gln Thr
20 25 30
Phe Ser Val Phe Leu Trp Thr Thr Ala Leu Pro Leu Ser Leu Gly Ile
35 40 45
Phe Cys Ile Leu Cys Ser Phe Pro Pro Leu Trp Pro Leu Val Ile Gly
50 55 60
Tyr Leu Thr Trp Val Phe Leu Ile Asp Gln Ala Pro Met Arg Gly Gly
65 70 75 80
Arg Pro Gln Ala Trp Leu Arg Lys Ser Arg Val Trp Glu Trp Phe Ala
85 90 95
Gly Tyr Tyr Pro Val Ser Leu Ile Lys Ser Ala Asp Leu Pro Pro Asp
100 105 110
Gln Arg Tyr Val Phe Gly Tyr His Pro His Gly Val Ile Gly Met Gly
115 120 125
Ala Ile Ala Asn Phe Gly Thr Asp Ala Thr Gly Phe Ser Arg Leu Phe
130 135 140
Pro Gly Ile Lys Pro His Leu Leu Thr Leu Ala Ser Asn Phe Lys Leu
145 150 155 160
Pro Leu Tyr Arg Glu Leu Leu Leu Ala Leu Gly Ile Ser Ser Val Ser
165 170 175
Met Lys Ser Cys Gln Asn Ile Leu Arg Gln Gly Pro Gly Ser Ser Ile
180 185 190
Thr Ile Val Val Gly Gly Ala Ala Glu Ser Leu Ser Ala His Pro Gly
195 200 205
Thr Ala Asp Leu Thr Leu Lys Arg Arg Lys Gly Phe Ile Lys Leu Ala
210 215 220
Ile Arg Ser Gly Ala Tyr Leu Val Pro Val Phe Ser Phe Gly Glu Asn
225 230 235 240
Asp Ile Phe Asn Gln Leu Ser Asn Glu Arg Gly Thr Arg Leu Tyr Lys
245 250 255
Leu Gln Lys Arg Phe Gln Ala Val Phe Gly Phe Thr Leu Pro Ile Phe
260 265 270
Phe Gly Arg Gly Leu Phe Asn Tyr Asn Met Gly Leu Met Pro Tyr Arg
275 280 285
His Pro Ile Val Ser Val Val Gly Arg Pro Ile Lys Val Thr Gln Lys
290 295 300
Asp His Pro Ser Thr Ala Asp Leu Glu Glu Val Gln Asp Arg Tyr Ile
305 310 315 320
Ala Glu Leu Lys Arg Ile Trp Glu Asp Tyr Lys Glu Val Tyr Ala Lys
325 330 335
Ser Arg Thr Lys Glu Leu Thr Ile Ile Ala
340 345
<210> 134
<211> 1041
<212> DNA
<213> 双倒卵形红冬孢酵母
<400> 134
atgggagcac tagatgcggg cgaccacgag gggaccgaac accccaagat caagttcgtt 60
cctttcgttg tgccgcgaca ccgcaggctg cagacctttt cggtgtttct gtggacgacc 120
gcgctgcctc tgtcgctcgg catcttctgc attctctgct ccttcccccc actctggccc 180
ctcgtcatag ggtatctcac gtgggtattc ctcatcgacc aggcgcccat gcggggtggc 240
aggcctcagg cctggttgcg caagtcgcgt gtgtgggagt ggttcgccgg ctactaccct 300
gtcagcttga tcaagagcgc cgacctcccg cccgaccagc gctacgtctt tggctaccac 360
ccacacggcg tcattgggat gggcgccatc gccaactttg gtaccgacgc gaccggcttc 420
tcgcggctgt tccccggcat caagccgcac ctcctcacgc tcgccagcaa cttcaagctg 480
ccgctctacc gagaactgct cctcgccttg ggcatttcgt ccgtgtcgat gaagagctgc 540
cagaacatcc tgcgccaagg tcccggctcg tcgatcacga ttgtcgtcgg aggggcagca 600
gaaagcctca gcgcgcaccc gggaacggca gacctgacgc tcaagcggcg gaaggggttc 660
atcaagctcg cgatccgctc aggggcctac ctcgtcccgg tattttcctt tggcgagaat 720
gacatcttca accagctgtc gaatgagcgc ggcacccgac tctacaagct gcaaaagcgg 780
ttccaggccg tctttggctt caccttgccc atcttcttcg gtcgcggcct cttcaactac 840
aacatgggct tgatgccata tcgacacccg atcgtctcgg tcgtcggacg ccccatcaag 900
gtcacgcaga aggatcaccc gtcgacggcc gacctcgaag aggtacagga ccgctacatt 960
gccgagttga agaggatctg ggaggactac aaagaggtgt acgccaagag ccgcaccaag 1020
gagctcacca tcatcgcatg a 1041
<210> 135
<211> 359
<212> PRT
<213> 三角褐指藻
<400> 135
Met Lys Glu Arg Arg Ser Gly Leu Asn Pro Ser Gly Ser Ser Val Tyr
1 5 10 15
Pro Leu His Pro Pro Asp Ser Arg Val Leu Val Arg Val Pro Ser Asp
20 25 30
Ile Ser Phe Leu Asp Arg Leu Ile Val Ala Gly Ser Ser Ile Phe Ile
35 40 45
Val Gly Ser Leu Val Trp Val Pro Leu Thr Ala Arg Trp Val Tyr Arg
50 55 60
Arg Trp Lys Gln Ala Lys Asp Lys Arg Lys Arg Ala Met Tyr Ala Ser
65 70 75 80
Leu Leu Val Ile Leu Ala Val Leu Val Ile Gly Gly Pro His Arg Ser
85 90 95
Pro Arg Val Gly Lys Trp Leu Gln Val Arg Lys Trp Ser Leu Phe Gln
100 105 110
Ala Trp Val Lys Phe Ile Ala Met Glu Val Ile Leu Asp Gln Pro Lys
115 120 125
Gly Ile Thr Met Asp Val Gln Gln Asp Lys Ala Ile Phe Ala Phe Ala
130 135 140
Pro His Gly Ile Phe Pro Phe Ala Phe Ala Phe Gly Val Leu Pro Asp
145 150 155 160
Ile Ala Thr Gln Ser Phe Gly Tyr Val Arg Pro Val Val Ala Thr Ala
165 170 175
Thr Arg Leu Phe Pro Val Val Arg Asp Phe Ile Ser Trp Ala Asn Pro
180 185 190
Val Asp Ala Ser Lys Asp Ser Val Glu Arg Ala Leu Ala Leu Gly Asp
195 200 205
Arg Ile Ala Val Ile Pro Gly Gly Ile Ala Glu Ile Phe Glu Gly Tyr
210 215 220
Pro Lys Pro Asn Thr His Pro Asp Glu Glu Tyr Ala Ile Val Arg Ser
225 230 235 240
Gly Phe Leu Arg Leu Ala Ile Lys His Gly Ile Pro Val Ile Pro Val
245 250 255
Tyr Cys Phe Gly Ala Thr Lys Met Leu Lys Arg Leu Glu Leu Pro Gly
260 265 270
Leu Glu Gln Leu Ser Leu Phe Leu Arg Val Ser Ile Cys Leu Phe Phe
275 280 285
Gly Val Gly Gly Leu Pro Ile Pro Phe Arg Gln Arg Leu Ser Tyr Val
290 295 300
Met Gly Gln Pro Ile Leu Pro Pro Val Arg Thr Thr Gly Ser Asp Ile
305 310 315 320
Ser Asp Ala His Val Lys Glu Met Gln Asp Arg Phe Cys Ala Glu Val
325 330 335
Gln Arg Leu Phe Asp Arg His Lys Glu Ala Tyr Gly Trp Ser Tyr Lys
340 345 350
Thr Leu Lys Leu Leu Glu Gln
355
<210> 136
<211> 1080
<212> DNA
<213> 三角褐指藻
<400> 136
atgaaagaaa gaagatctgg cctaaatccg tcaggatcct ccgtgtatcc attgcaccct 60
cctgacagtc gcgttctcgt tcgagtcccc tccgatattt cctttcttga tcgtctcatc 120
gtcgctggca gcagtatctt tattgtcggt tcgctagtat gggttccatt gaccgcaaga 180
tgggtctaca ggcggtggaa gcaagctaaa gataaacgaa agcgggctat gtatgcctct 240
ctactcgtga ttctggcagt tctcgttatt ggcggacccc accgatctcc tcgtgtcggc 300
aaatggctcc aagtacgaaa gtggtccctc ttccaagcgt gggtaaagtt tattgccatg 360
gaagtgattt tggatcaacc gaaaggcatt actatggacg tccaacaaga caaggcgatt 420
tttgcattcg cgccacatgg aatctttccg tttgcgttcg cctttggagt gcttcccgat 480
attgccacac aatcgtttgg ctacgttcgt ccggtcgtgg caaccgccac aaggttgttt 540
cctgtagtcc gggatttcat ctcttgggcg aatccggtag acgcttccaa agattccgtt 600
gaacgtgctt tagcattggg cgatcgcatt gctgtaatac ctggaggaat tgcagaaatt 660
ttcgaaggat atccgaaacc gaacacgcat ccggatgaag agtacgctat cgtacggagt 720
ggatttttgc gtttggcaat aaaacacggt atcccagtga ttcccgtata ctgtttcggc 780
gctaccaaaa tgttgaagcg tctggagctt cccggcctgg agcaactgtc cctgtttcta 840
cgcgtgagca tttgcctctt ttttggagtc ggcgggttgc ccatcccttt ccgacaacga 900
ttgtcgtacg taatgggaca accaattttg ccacccgtaa ggacaacggg cagcgatatt 960
tcggacgcac acgtcaaaga aatgcaagat cgcttttgtg ctgaggtcca gcggctcttt 1020
gatcgacata aggaagctta tggttggtca tacaaaacgc tgaaactatt ggaacagtga 1080
<210> 137
<211> 329
<212> PRT
<213> 三角褐指藻
<400> 137
Met Glu Arg Thr Lys Ile Gln Asp Glu His Lys Ser Pro Pro Asn Pro
1 5 10 15
Ser Thr Phe Arg Trp Phe Leu Gly Leu Leu Val Ala Ser Thr Phe Ser
20 25 30
Met Val Tyr Phe Val Ala Pro Phe Tyr Met Leu Thr Val Val Phe Ala
35 40 45
Leu Val Phe Lys Tyr Pro Ser Val Glu Ile Ala Trp Met Tyr Ala Ile
50 55 60
Pro Met Ile Val Ser Ala Ile Leu Pro Pro Met Ala Ser Pro Leu Ala
65 70 75 80
Leu Arg Leu Ile Ser Pro Leu Ile Asp Tyr Phe Asp Tyr Glu Glu Ile
85 90 95
His Glu Thr Ser Pro Val Asp Val Gln Lys Glu Ile Leu Ser Asn Asn
100 105 110
Lys Asn Tyr Leu Leu Val Phe Gln Pro His Gly Ala Leu Ser Phe Thr
115 120 125
Gly Ile Thr Ser Met Val Thr Ala Pro Gln Ala Met Lys Gly Lys Leu
130 135 140
Pro Thr Ala Val Ala Asp Ala Leu Leu Tyr Thr Pro Ile Leu Lys His
145 150 155 160
Val Leu Gly Ile Phe Gly Leu Ile Ser Ala Ser Lys Ser Ser Met Ile
165 170 175
Arg Thr Leu Lys Lys Lys Gly Val Glu Gly Thr Ile Val Leu Tyr Val
180 185 190
Gly Gly Ile Ala Glu Leu Phe Leu Thr Asp Glu Thr Asp Glu Arg Leu
195 200 205
Tyr Leu Arg Lys Arg Lys Gly Phe Ile Lys Leu Ala Leu Gln Gln Gly
210 215 220
Val Asp Val Val Pro Val Tyr Leu Phe Gly Asn Thr Asn Ala Leu Ser
225 230 235 240
Val Leu Lys Thr Gly Phe Leu Ala Ala Ile Ser Arg Lys Leu Gln Ile
245 250 255
Ser Leu Thr Tyr Ile Trp Gly Lys Trp Tyr Leu Pro Ile Pro Arg Asp
260 265 270
Cys Lys Leu Leu Tyr Ala Ser Gly Gln Pro Leu Gly Met Pro His Ile
275 280 285
Leu Asp Pro Ser Gln Ala Asp Ile Asp Lys Trp His Glu Lys Tyr Cys
290 295 300
Ser Glu Val Met Arg Ile Phe Glu Lys Tyr Lys Glu Lys Val Pro Glu
305 310 315 320
Tyr Lys His Lys Lys Leu Glu Ile Ile
325
<210> 138
<211> 990
<212> DNA
<213> 三角褐指藻
<400> 138
atggagagaa caaagataca agacgagcac aaaagtcccc ctaatccgtc gacatttcga 60
tggttcctcg gccttctagt ggcgtcgacg ttttccatgg tctattttgt ggctcccttt 120
tacatgctta cagtcgtgtt tgcactagtt ttcaaatatc cttcggtaga aattgcatgg 180
atgtacgcta ttccgatgat tgtctcggcc attttgccac caatggcttc tccactggcc 240
ttgcgactca tctccccgct cattgactac ttcgattacg aagagatcca cgaaacctca 300
ccggtggacg tccagaagga aatactaagc aacaacaaaa actatttgct agtctttcaa 360
ccgcatggag cactgtcgtt tacaggaatc acttcaatgg tgacagctcc acaagcaatg 420
aaaggcaaat tgccaacagc tgtggctgac gcactcttgt acacacctat actgaaacat 480
gtcttaggaa ttttcgggct gattagtgcc tccaaaagca gcatgatccg aactttaaaa 540
aagaagggtg tggaaggaac cattgttttg tacgttggtg ggattgccga gctctttttg 600
accgacgaga cggacgagcg cctctatctg cgaaagcgaa aagggtttat caaattagct 660
ctacaacagg gtgtcgatgt tgtacctgtg tatctatttg ggaacacaaa cgcgctgtcg 720
gtactaaaga cgggatttct cgcggcaatt tcgcgaaaat tacagatatc tctgacgtac 780
atttggggaa agtggtatct tccgattccc cgtgattgca aattgctgta tgcttccggt 840
cagccattag gaatgcctca tattttagac ccaagccaag ccgacattga taaatggcac 900
gaaaagtact gctccgaggt catgcggatc ttcgaaaaat acaaggaaaa ggttccggaa 960
tacaagcaca agaaattaga aattatttga 990
<210> 139
<211> 392
<212> PRT
<213> 三角褐指藻
<400> 139
Met Arg Glu Arg Ser Cys Ala Asn Ala Ser Asp Asp Asp Ser Ile His
1 5 10 15
Lys Gln Ser Pro Glu Leu Glu Ala Glu Phe Leu His Thr Ser Lys Leu
20 25 30
Thr Leu Ala Asp Met Arg Arg Leu Ala His Asp Pro Lys Asp Arg Gly
35 40 45
Leu Ala Thr Lys Pro Ala Ala Gln Ala Thr Lys Glu Asp Val Leu Thr
50 55 60
Val Gln Pro Met Ser Phe Val Glu His Thr Ala Cys Cys Leu Phe Leu
65 70 75 80
Ala Phe Gly Val Pro Asn Gly Ala Leu Thr Ile Pro Ile Ala Thr Trp
85 90 95
Leu Ile Gly Lys Phe Val Leu Arg Asn Val Phe Leu Ala Phe Leu Leu
100 105 110
Ala Gly Cys Ile Leu Leu Pro Leu Ala Ile Leu Pro Gln Glu Tyr Val
115 120 125
Pro Ala Arg Leu Gln Ser Trp Leu Ala Leu Gln Ile Leu Lys Tyr Phe
130 135 140
Ser Phe Ser Leu Val Met Glu Glu Arg Pro Pro Thr Met Cys Thr Gly
145 150 155 160
Lys Gln Leu Ile Glu Gln Pro Ala Arg Pro Arg Ile Val Thr Ala Tyr
165 170 175
Pro His Gly Val Phe Pro Tyr Gly Asn Ala Leu Thr Val Val Thr Trp
180 185 190
Pro Leu Leu Thr Gly His His Ile Val Gly Leu Ala Ala Asn Ala Ala
195 200 205
Leu Arg Thr Pro Ile Phe Lys Gln Ile Leu Arg Ser Ile Gly Val Lys
210 215 220
Asp Ala Ser Arg Ala Ser Val Arg Asn Ala Leu Glu Thr Trp Pro Phe
225 230 235 240
Thr Val Gly Ile Ser Pro Gly Gly Val Ala Glu Val Phe Glu Thr Asn
245 250 255
His Phe Asn Glu His Ile Leu Leu Lys Glu Arg Ile Gly Val Ile Lys
260 265 270
Met Ala Ile Arg Thr Gly Ala Asp Leu Val Pro Gly Tyr Met Tyr Gly
275 280 285
Asn Thr Asn Leu Tyr Trp Cys Trp Thr Gly Glu Gly Ile Pro Gly Ala
290 295 300
Arg Trp Leu Leu Glu Tyr Val Ser Arg Lys Ile Leu Gly Phe Ala Leu
305 310 315 320
Val Pro Ile Ala Gly Arg Trp Arg Leu Pro Ile Pro Tyr Arg Thr Pro
325 330 335
Ile Leu Cys Val Val Gly Lys Pro Ile Pro Thr Ile His Leu Gln Thr
340 345 350
Glu Glu Pro Ser Met Glu Gln Ile Val Asp Ile Gln Glu Gln Leu Ser
355 360 365
Thr Glu Leu Lys Ser Met Phe Asp Arg Tyr Lys His Leu Tyr Gly Trp
370 375 380
Glu Asp Arg Met Leu Val Ile Thr
385 390
<210> 140
<211> 1179
<212> DNA
<213> 三角褐指藻
<400> 140
atgcgtgagc gaagctgcgc caacgcttct gacgatgaca gcattcacaa gcagtcgcca 60
gaattggagg ctgagtttct tcataccagc aagttgacct tagccgacat gcgacgattg 120
gcgcacgatc cgaaggatcg ggggttggca acaaaacctg cggcgcaagc tacgaaagaa 180
gacgtcttga cggtacaacc catgagtttc gtagaacaca ctgcttgctg tctgtttctc 240
gcgtttggag tgcccaatgg cgctctgacg attcccatag caacgtggct gatcggaaaa 300
ttcgtgttgc gcaacgtttt cttggcgttt ctgttagcag gctgtatact tctaccgctt 360
gcgatactgc cgcaagaata tgtgcccgcc cgattgcaat cgtggcttgc tttgcagata 420
ctgaaatatt tttctttctc tttggtcatg gaggaacgcc ctccgacaat gtgtactggc 480
aagcagctga tcgagcagcc cgctcggcca cgaatcgtca cagcctatcc gcacggagtt 540
ttcccatacg gaaacgcgtt gactgtagtc acatggccgt tgttgacggg acaccatatt 600
gtgggtttgg cagcaaatgc cgctttgcgg acaccgatct ttaaacaaat cttgcggagc 660
attggcgtca aggacgcctc tcgagcgtcg gtacggaatg cgctggaaac atggcctttc 720
accgtcggga tttcgccagg tggcgtggcg gaagtttttg aaacaaacca cttcaatgag 780
cacattctgt tgaaagaacg tattggtgtc atcaagatgg ccattcgcac cggtgcggat 840
cttgtaccag gctatatgta tggtaatact aatctgtact ggtgctggac aggggaaggt 900
attcctggag ctcggtggct attggagtat gtttcgcgta aaatcctagg ttttgccctc 960
gtgcctatag cgggtagatg gagactacca ataccgtaca ggactccgat attgtgtgtc 1020
gtgggcaagc caataccaac cattcatttg caaaccgaag aaccatcaat ggagcaaatc 1080
gtggacattc aggaacaatt gtcaacagaa ttgaaatcaa tgttcgaccg ctataagcac 1140
ctgtacggat gggaagatcg aatgctagtg atcacataa 1179
<210> 141
<211> 320
<212> PRT
<213> 三角褐指藻
<400> 141
Met Thr Arg Ser Lys Phe Ile Gly Ser Ala Gly Ala Ile Gly Leu Phe
1 5 10 15
Cys Leu Met Ile Ile Pro Asn Val Gly Ile Leu Ile Ala Thr Phe Leu
20 25 30
Tyr Pro Lys Val Leu Gly Leu Tyr Phe Leu Ile Pro Tyr Tyr Ala Tyr
35 40 45
Asn Leu Ser Ile Gly Lys His Glu Ala Arg Asp Gly Asn Gly Trp Asn
50 55 60
Trp Phe Ser Glu Asn Phe Phe Val Phe Asn Ile Val Arg Gly Tyr Leu
65 70 75 80
Asn Leu Lys Ile Glu Ala Asp Ser Glu Leu Lys Glu Ala Glu Ala Lys
85 90 95
Glu Gly Ala Gln Phe Val Phe Ala Val Ser Pro His Gly Thr Asn Ala
100 105 110
Asp Tyr Arg Val Phe Ile Asp Gly Met Leu His Glu Ala Leu Pro Gln
115 120 125
Thr Ala Ser Lys Ile Arg Thr Leu Ala Ala Thr Val Leu Phe His Ile
130 135 140
Pro Leu Val Arg Glu Ile Ala Leu Trp Thr Gly Cys Val Asp Ala Ser
145 150 155 160
Arg Ala Val Ala Val Glu Arg Leu Lys Glu Glu Gly Gly Ser Leu Leu
165 170 175
Val Ile Pro Gly Gly Gln Ala Glu Gln Met Tyr Thr Gln Tyr Gly Arg
180 185 190
Glu Arg Val Tyr Leu Lys Arg Arg Lys Gly Phe Leu Lys Leu Cys Leu
195 200 205
Lys Tyr Glu Ile Pro Val Val Pro Ala Tyr Val Phe Gly Val Ser Asp
210 215 220
Tyr Tyr Phe Thr Ser Ala Lys Leu Phe Gly Leu Arg Met Trp Leu Val
225 230 235 240
Gln Asn Leu Gly Ile Ala Leu Pro Leu Cys Trp Gly Arg Tyr Gly Leu
245 250 255
Pro Ile Cys Pro Arg Pro Val Asp Thr Thr Leu Val Phe Asp Lys Pro
260 265 270
Leu Tyr Leu Ser Cys Gln Asn Pro Ser Asn Pro Ser Glu Asp Glu Val
275 280 285
Asp Lys Ala His Leu Gln Phe Cys Gln Ala Leu Glu Lys Leu Phe Asp
290 295 300
Thr His Lys Glu Arg Leu Gly Tyr Gly Asp Arg Lys Leu Glu Ile Ile
305 310 315 320
<210> 142
<211> 963
<212> DNA
<213> 三角褐指藻
<400> 142
atgaccagat cgaagtttat aggaagtgct ggagctattg gcttattttg tttgatgatc 60
ataccgaatg tgggaattct gatcgcaaca tttctttatc ccaaagtact tgggctctac 120
tttctgattc cgtactacgc atacaacttg tccattggca aacacgaagc tcgagacggc 180
aacggctgga attggttcag cgagaatttc tttgtcttta acattgtgag gggatatcta 240
aatcttaaga ttgaagctga ctccgagctc aaggaagccg aagcgaaaga aggcgcccaa 300
tttgtgttcg ccgttagccc tcacggaacg aacgcagact atcgagtttt tattgacggt 360
atgctacatg aggcactccc acagactgca agcaagatca gaacactagc ggcgacagta 420
ctgttccaca ttcccttggt tcgtgaaatc gcactttgga caggatgtgt cgatgccagc 480
cgcgcagttg ctgtcgagag attaaaagaa gaaggtggtt cactgcttgt gattcccggt 540
ggccaagcag aacaaatgta cacccaatat ggacgtgaaa gagtatatct gaaacggcgc 600
aaaggatttt tgaagctttg cttgaagtac gagattccgg tcgtcccagc ttatgttttt 660
ggcgtatctg actattactt cacgtccgca aagctctttg gtctgcgaat gtggctcgtt 720
cagaatcttg gcattgctct tccactgtgc tggggaagat atggtctacc aatctgtcct 780
agaccagtcg ataccaccct tgtctttgac aaacctttat acctatcctg ccagaatccg 840
tcgaatccct cggaagacga ggttgacaag gctcatctgc aattttgcca agccctcgag 900
aagctgtttg atacacacaa agagaggctt gggtacggcg atcgaaagct ggaaataatt 960
tag 963
<210> 143
<211> 564
<212> PRT
<213> 三角褐指藻
<400> 143
Met Asp Glu Thr Glu Ile Thr Pro Leu Leu Arg Phe Ser Thr Pro Ser
1 5 10 15
Arg Ala Glu His Ser Ser Trp Ile Lys Leu Ala Ser Glu Ser Cys Ala
20 25 30
Tyr Ser Glu Thr Asp Glu Phe Leu Ala Asp Glu Ala Ala Arg Ala Thr
35 40 45
Gln Arg Ala Leu Gln His Gln Glu Ala Leu Gln Met Ala Gln Ala Met
50 55 60
Pro Gly Ala Lys Pro Gly Thr Leu Pro Pro Leu Tyr Phe Ala Pro Thr
65 70 75 80
Ile Lys Arg Ser Arg Ser Phe Ala Lys Leu Gln Glu His His Gly Asp
85 90 95
Gly Met Pro Arg Val Asn Met Arg Arg Thr Lys Ser Arg Asp Phe Asn
100 105 110
Ala Asp Lys Leu Asp Ala Arg Ser Thr Lys Gly Tyr Pro Pro Ser Lys
115 120 125
Pro Met His Arg Ala Ala Glu Pro Ser Tyr Leu Ser Ala Asp Ala Pro
130 135 140
Ile Gln Asn Tyr Arg Gly Phe Leu Asn Leu Gly Val Ile Ile Leu Ile
145 150 155 160
Val Ser Asn Phe Arg Leu Ile Leu Gly Thr Ile Arg Ser Asn Gly Phe
165 170 175
Val Leu Thr Thr Ala Val Lys His Tyr Lys Asn Leu Asn His Leu Lys
180 185 190
Glu Asp Pro Trp Gln Glu Phe Pro Phe Val Ser Gly Phe Leu Leu Gln
195 200 205
Leu Val Phe Val Ser Ile Ala Phe Gly Ile Glu Trp Met Leu Cys Arg
210 215 220
Lys Tyr Phe Asn Glu Asn Phe Gly Met Ile Leu His His Phe Asn Ala
225 230 235 240
His Ser Ala Leu Leu Ile Pro Leu Gly Ile Val Trp Asn Leu Ile Asp
245 250 255
Arg Pro Ala Val Gly Ala Ile Leu Leu Leu His Ala Thr Ile Thr Trp
260 265 270
Met Lys Leu Ile Ser Tyr Met Leu Ala Asn Glu Asp Tyr Arg Leu Ser
275 280 285
Ser Arg Arg Val Gly Gly Asn Pro His Leu Ala Thr Leu Ala Leu Val
290 295 300
Glu Asn Leu Asp Ser Asp Glu Ala Asn Ile Asn Tyr Pro Gln Asn Val
305 310 315 320
Thr Leu Arg Asn Ile Phe Tyr Phe Trp Cys Ala Pro Thr Leu Thr Tyr
325 330 335
Gln Ile Ala Phe Pro Lys Ser Pro Arg Val Arg Tyr Trp Lys Ile Ala
340 345 350
Asp Ile Leu Met Arg Met Thr Val Ser Ile Ala Leu Phe Thr Phe Leu
355 360 365
Leu Ala Gln Ile Val Gln Pro Ala Leu Glu Glu Leu Val Ser Asp Leu
370 375 380
Asp Glu Thr Asn Gly Ser Tyr Thr Ala Ala Ile Phe Ala Glu Tyr Trp
385 390 395 400
Leu Lys Leu Ser Ile Ala Asn Thr Tyr Leu Trp Leu Leu Met Phe Tyr
405 410 415
Thr Tyr Phe His Leu Tyr Leu Asn Leu Phe Ala Glu Leu Leu Arg Phe
420 425 430
Gly Asp Arg Val Phe Tyr Lys Asp Trp Trp Asn Ser Ser Glu Val Ser
435 440 445
Ala Tyr Trp Arg Leu Trp Asn Met Pro Val His Tyr Trp Leu Ile Arg
450 455 460
His Val Tyr Phe Pro Cys Val Arg Leu Lys Met Pro Lys Val Ala Ala
465 470 475 480
Thr Phe Val Val Phe Phe Leu Ser Ala Val Met His Glu Val Leu Val
485 490 495
Ser Val Pro Phe His Ile Ile Arg Pro Trp Ser Phe Ile Gly Met Met
500 505 510
Met Gln Ile Pro Leu Val Ala Phe Thr Lys Tyr Leu Tyr Arg Lys Phe
515 520 525
Pro Gly Gly Ser Ile Gly Asn Val Leu Phe Trp Met Thr Phe Cys Val
530 535 540
Ile Gly Gln Pro Met Ala Ile Leu Leu Tyr Tyr His Asp Ile Met Asn
545 550 555 560
Arg Lys Gly Asn
<210> 144
<211> 1695
<212> DNA
<213> 三角褐指藻
<400> 144
atggatgaga ccgaaattac acctttgttg cgtttttcga caccttcccg agccgaacac 60
tcgtcctgga taaagcttgc ctcggaatcc tgtgcttaca gcgaaacgga cgagtttctc 120
gctgacgagg ccgctcgcgc aacccagcgt gctttgcaac atcaagaagc gctgcaaatg 180
gcccaagcca tgcctggggc aaagccagga acgctgccgc cactctactt cgcgcctacc 240
ataaagcgtt cgcgttcctt tgctaagcta caagaacatc atggagatgg gatgcctcgg 300
gtaaatatgc gtcggaccaa atcgcgagat tttaacgcgg ataagttgga tgcgcgaagt 360
accaagggct atcccccttc caagccgatg catcgtgcgg cagagccctc atacctcagc 420
gcggatgctc ccattcaaaa ctaccgagga tttctgaatt taggcgttat tattttgatt 480
gtttctaact ttcggctgat cttgggcaca atccgtagca acggatttgt cttgacgact 540
gcagtgaagc actacaagaa cctaaatcac ctcaaggaag atccctggca ggaatttcct 600
tttgtatcag gatttcttct ccagctcgtc tttgtttcga ttgcgtttgg gatcgaatgg 660
atgttgtgcc ggaaatactt caacgaaaac ttcggcatga tccttcatca cttcaatgcc 720
cactcagcct tgctgatacc tttaggtatt gtttggaatc tcatcgatag acctgcggtt 780
ggtgcaattt tgcttttaca cgctacgata acatggatga aactcatttc ttacatgttg 840
gcgaacgaag attaccggct atcatcgcgt cgcgttgggg gcaacccaca cctagctacg 900
ctcgcattag tcgaaaatct agattcagat gaggcgaaca ttaactaccc ccaaaatgtt 960
actctccgca acatttttta tttttggtgt gctccgacgt tgacttacca gattgccttc 1020
ccgaagtccc cgcgagttcg ctattggaaa atcgcggata tcctgatgcg catgacggtg 1080
tccatcgcac tattcacctt tttgctggca caaattgttc agcctgcatt ggaagagcta 1140
gtgagcgacc tggacgagac caatggatcc tacaccgcag caatatttgc cgagtactgg 1200
ctgaaacttt cgattgctaa cacatattta tggcttctta tgttctatac atatttccat 1260
ttgtatctga acctctttgc tgagcttctg cgatttggag atcgtgtgtt ctacaaagat 1320
tggtggaatt cgtcggaagt atctgcatat tggaggcttt ggaatatgcc tgttcactat 1380
tggttgatcc gacatgtgta tttcccctgc gtgcgactga agatgccgaa ggtcgctgca 1440
acctttgtcg tttttttcct ctccgccgtt atgcacgagg tgcttgtcag cgtacccttt 1500
catattattc gtccgtggtc ttttatcggg atgatgatgc agattccttt ggttgcgttc 1560
acaaagtatc tctatcgcaa attcccgggc ggctcgattg gtaatgtcct gttctggatg 1620
acattttgcg tcattggcca gccaatggcg attctcttgt actatcatga tattatgaat 1680
cgaaaaggaa attga 1695
<210> 145
<211> 503
<212> PRT
<213> 蝗绿僵菌
<400> 145
Met Ser Thr Ala Thr Thr Thr Ser Val Ser Pro Ala Asn Gly Thr Val
1 5 10 15
Ser Lys Arg Asn Ala Thr Lys Arg Arg Asn Gly Asn Ala Ser Pro Gly
20 25 30
Pro Val Glu Glu Glu Ser Glu Asp Ala Ala Ala Ala Glu Lys Pro Arg
35 40 45
Ala Ser Val Ala Gln Lys Asn Tyr Arg His Val Ala Ala Val His Ser
50 55 60
Lys Ser Arg Pro Ser Cys Leu Ser His Asp Ser Asp Ala Thr Pro Ser
65 70 75 80
Phe Ile Gly Phe Arg Asn Leu Met Val Ile Val Leu Asp Val Leu Ile
85 90 95
Gly Gly Leu Leu Tyr Phe Leu Ile Pro Cys His Leu Leu Val Ala Tyr
100 105 110
Leu Ile Glu Leu Ala Ala Ala Lys Gln Ala Arg Gly Ser Arg Lys Arg
115 120 125
Leu Lys Pro Gly Ser Thr Val Pro Ser Glu Gln Asp Asn Ser Lys Phe
130 135 140
His Ser Thr Trp Val Leu Val Ala Trp Ala His Gly Ile Asn Met Thr
145 150 155 160
Leu Ala Leu Ala Leu Thr Thr Phe Met Val Tyr Phe Tyr Ile His His
165 170 175
Pro Leu Val Gly Thr Leu Thr Glu Met His Ala Val Ile Val Ser Leu
180 185 190
Lys Thr Ala Ser Tyr Ala Phe Thr Asn Arg Asp Leu Arg His Ala Tyr
195 200 205
Leu His Pro Val Lys Gly Glu Phe Ile Pro Glu Leu Tyr Ser Lys Cys
210 215 220
Pro Tyr Pro Asn Asn Ile Thr Phe Gly Asn Leu Ala Tyr Phe Trp Trp
225 230 235 240
Ala Pro Thr Leu Val Tyr Gln Pro Val Tyr Pro Arg Thr Asp Lys Ile
245 250 255
Arg Trp Val Phe Val Phe Lys Arg Leu Gly Glu Val Cys Cys Leu Ser
260 265 270
Ala Phe Ile Trp Phe Ala Ser Phe Gln Tyr Ala Ala Pro Val Leu Gln
275 280 285
Asn Ser Leu Asp Lys Ile Ala Ser Leu Asp Leu Leu Met Ile Leu Glu
290 295 300
Arg Leu Leu Lys Leu Ser Thr Ile Ser Leu Val Ile Trp Leu Ala Gly
305 310 315 320
Phe Phe Ala Leu Phe Gln Ser Phe Leu Asn Ala Leu Ala Glu Val Leu
325 330 335
Arg Phe Gly Asp Arg Ser Phe Tyr Asp Asp Trp Trp Asn Ser Glu Ser
340 345 350
Leu Gly Ala Tyr Trp Arg Thr Trp Asn Arg Pro Val Tyr Thr Tyr Phe
355 360 365
Lys Arg His Val Tyr Val Pro Met Ile Gly Arg Gly Trp Ser Pro Trp
370 375 380
Ala Ala Ser Cys Ala Val Phe Phe Val Ser Ala Val Leu His Glu Val
385 390 395 400
Leu Val Gly Val Pro Thr His Asn Ile Ile Gly Thr Leu Ser Ser Val
405 410 415
Leu Ser Ile Val Leu Thr Leu Val Pro Asn Leu Tyr Ser Gly Val Ala
420 425 430
Phe Leu Gly Met Phe Leu Gln Leu Pro Leu Ile Ala Ile Thr Ala Pro
435 440 445
Leu Glu Lys Met Lys Trp Gly His Thr Gly Arg Val Met Gly Asn Val
450 455 460
Ile Phe Trp Val Ser Phe Thr Ile Phe Gly Gln Pro Phe Ala Ala Leu
465 470 475 480
Met Tyr Phe Tyr Ala Trp Gln Ala Lys Tyr Gly Ser Val Ser Lys Glu
485 490 495
Pro Ile Leu Ala Leu Gln Thr
500
<210> 146
<211> 1512
<212> DNA
<213> 蝗绿僵菌
<400> 146
atgagcacgg ccaccaccac cagtgtcagc ccagcgaatg gcaccgtgag caagagaaat 60
gccaccaagc gtcgcaacgg caatgcatct cccggcccgg tggaagaaga atccgaagac 120
gcagccgcag ccgagaagcc cagagcctct gtcgcccaga agaactatcg ccacgtagca 180
gcagtgcatt ccaagagccg cccgtcgtgc ctaagccacg actccgatgc cacgccaagc 240
tttatcgggt ttcgaaatct catggtcatt gttttggatg tcctcatcgg cggacttctc 300
tactttctca ttccctgcca tctgttggtt gcctacttga tcgaattggc cgccgcaaaa 360
caggctcgag gatcccgaaa gcgcctcaaa ccaggctcta ctgtaccgtc ggaacaagac 420
aattccaagt tccattcaac atgggttctg gtggcctggg ctcatggtat caatatgacg 480
cttgctttag ccctcacaac ctttatggtt tacttttaca tccaccaccc gctcgttggg 540
accctgaccg agatgcatgc cgtcattgtg tcgttgaaga cagcctcgta cgcattcacc 600
aaccgagatc ttcgccacgc ttacctgcac cccgttaaag gagagtttat tcctgaactc 660
tactcgaaat gcccgtaccc gaataacatc acctttggca acctcgccta cttctggtgg 720
gcgccgacgc tggtctatca gcccgtatac ccgcgcaccg acaagatcag atgggtcttt 780
gtttttaaga ggctgggcga agtatgctgt ttgagcgcat tcatctggtt cgccagcttc 840
caatacgccg cgccggttct gcagaattcg ctcgacaaga ttgcttcgtt ggacttactc 900
atgatcctag agcggctgct gaagctgtca accatttctc tggttatttg gctggcagga 960
ttctttgccc tattccagtc cttcttaaac gcacttgccg aagtgctgcg gttcggcgac 1020
cgatcatttt acgacgactg gtggaacagc gagagtctcg gagcctactg gagaacgtgg 1080
aacaggcccg tatatacgta ctttaagcgc catgtgtatg tacccatgat tgggcgtgga 1140
tggagcccat gggctgcaag ttgcgccgtc ttttttgtgt ctgccgtgtt acacgaggtt 1200
cttgttggtg ttcccaccca caacattatc ggtacgctat cctccgtctt atccatcgtc 1260
ttgaccctcg ttcctaacct atattcaggc gttgcttttc taggcatgtt cttgcagctt 1320
cctctcatcg ccatcacggc ccctctagag aaaatgaaat gggggcatac cggcagagta 1380
atgggaaacg taatcttttg ggtgtccttt accatcttcg gtcagccatt tgcggcattg 1440
atgtactttt acgcatggca ggccaagtac ggtagcgtca gtaaagaacc gattcttgcg 1500
ttgcagacat ga 1512
<210> 147
<211> 516
<212> PRT
<213> 冬虫夏草
<400> 147
Met Ala Ala Thr Gly Thr Ser Val Glu Pro Ser Thr Gly Thr Ala Thr
1 5 10 15
Gln Arg His Ser Gly Lys Asp Gln Thr Gly Val Glu Pro Arg Thr Gly
20 25 30
Thr Val Lys Thr Ser Gln Lys Lys Tyr Arg His Val Val Val Val His
35 40 45
Ser Gln Val Arg Pro Ser Cys Leu Ser His Asp Ser Asp Ala Ala Pro
50 55 60
Ser Phe Ile Gly Phe Arg Asn Leu Met Val Ile Val Leu Val Val Gly
65 70 75 80
Asn Leu Arg Leu Met Ile Glu Asn Ile Gln Lys Ala Arg Ser Tyr Leu
85 90 95
Ser Phe Ile Pro Gly Gln Cys Ala Pro Gly Tyr Gly Val Leu Ile Cys
100 105 110
Ile Arg Cys His Ala Tyr Ser Arg Gln Asp Ile Leu Val Gly Gly Leu
115 120 125
Leu Tyr Ile Leu Ile Pro Cys His Leu Leu Ala Ala Tyr Leu Ile Glu
130 135 140
Leu Ala Ala Ala Gln Gln Ala Leu Gly Ser Arg Lys Arg Leu Lys Asp
145 150 155 160
Gly Ala Ala Ser Pro Glu Glu Glu Asp Arg Asn Ser Asn Lys Phe His
165 170 175
Ala Thr Trp Leu Ile Val Ala Trp Val His Ala Val Asn Ile Thr Leu
180 185 190
Ala Leu Val Val Thr Ser Ala Val Val Tyr Phe Tyr Ile His His Pro
195 200 205
Leu Ile Gly Thr Leu Thr Glu Met His Ala Ile Ile Val Trp Leu Lys
210 215 220
Thr Ala Ser Tyr Ala Phe Thr Asn Arg Asp Leu Arg His Ala Tyr Leu
225 230 235 240
His Pro Val Glu Gly Glu Leu Val Pro Asp Met Tyr Ala Lys Cys Pro
245 250 255
Tyr Pro Gln Asn Ile Thr Phe Gly Asn Leu Val Tyr Phe Trp Trp Ala
260 265 270
Pro Thr Leu Val Tyr Gln Pro Val Tyr Pro Arg Thr Asp Lys Ile Arg
275 280 285
Trp Leu Phe Val Ala Lys Arg Leu Gly Glu Val Phe Cys Leu Ser Ala
290 295 300
Phe Ile Trp Phe Ala Ser Phe Gln Tyr Ala Ala Pro Val Leu Arg Asn
305 310 315 320
Ser Leu Asp Lys Ile Ala Ser Leu Asp Phe Ala Ser Ile Phe Glu Arg
325 330 335
Leu Val Lys Leu Ser Thr Ile Ser Leu Val Ile Trp Leu Ala Gly Phe
340 345 350
Phe Ala Leu Phe Gln Ser Phe Leu Asn Ala Leu Ala Glu Val Leu Arg
355 360 365
Phe Gly Asp Arg Ala Phe Tyr Asp Asp Trp Trp Asn Ser Glu Ser Leu
370 375 380
Gly Ala Tyr Trp Arg Thr Trp Asn Lys Pro Val Tyr Thr Tyr Phe Lys
385 390 395 400
Arg His Val Tyr Met Pro Met Ile Gly Arg Gly Trp Ser Pro Arg Val
405 410 415
Ala Ser Leu Val Val Phe Phe Ile Ser Ala Val Leu His Glu Ile Leu
420 425 430
Val Gly Leu Pro Thr His Asn Val Ile Gly Val Ala Phe Leu Gly Met
435 440 445
Phe Leu Gln Leu Pro Leu Ile Ala Ile Thr Ala Pro Met Glu Lys Met
450 455 460
Arg Leu Gly Lys Gly Gly Lys Leu Val Gly Asn Val Ile Phe Trp Val
465 470 475 480
Ser Phe Thr Ile Phe Gly Gln Pro Phe Ala Thr Leu Met Tyr Phe Tyr
485 490 495
Ala Trp Gln Ala Lys Tyr Gly Ser Val Ser Arg Glu Met Gln Gln Ala
500 505 510
Ala Ser Ile Lys
515
<210> 148
<211> 1551
<212> DNA
<213> 冬虫夏草
<400> 148
atggcggcta cggggaccag cgtcgagccc tcgactggta ccgcgacaca acgccactcc 60
ggcaaggatc agactggggt cgagccacgc accggcacgg tcaagacatc ccagaaaaag 120
tatcgccatg tcgttgtcgt ccactcccag gtccggccct cgtgcctcag ccacgattca 180
gatgccgccc ccagcttcat tggcttccgc aatctcatgg ttattgtcct ggtcgtcggc 240
aacttgcgat tgatgattga aaacatccaa aaggctcgtt catacctgtc gttcatacct 300
ggccaatgcg cccccggcta cggagtcttg atctgcatcc gctgccacgc ctacagccgc 360
caagacattc tcgtcggcgg gctgctgtac atcctcattc cctgccatct cctggccgcc 420
tatctcatcg agctcgccgc cgcccagcag gcactggggt cgagaaagcg cctcaaggat 480
ggcgccgcca gcccggagga ggaggaccgc aacagcaaca agtttcacgc gacatggctc 540
atcgtcgcct gggtccatgc cgtcaacatc accctggccc tggtcgtgac ctcggccgtc 600
gtctactttt acatccacca cccactcatc ggcaccctca ccgaaatgca cgccatcatc 660
gtctggctca agacggcctc gtacgccttt actaaccgcg acctgcgcca cgcgtacctg 720
caccccgtcg agggcgagct cgtcccggac atgtacgcca agtgcccgta tccgcaaaac 780
atcacctttg gcaacctcgt ctacttctgg tgggccccga cgctcgtcta ccagcccgtc 840
tatccccgga ccgacaagat caggtggctc tttgtcgcca agcggctggg agaggtcttt 900
tgcttgagcg ccttcatctg gttcgccagc ttccagtatg ccgcgcccgt cctgcgcaac 960
tctctcgaca aaattgcttc gctcgacttt gcctccatct ttgagcggct ggtgaagctg 1020
tccaccatct ccctcgtcat ctggctcgcc ggcttcttcg ccctcttcca gtcctttctc 1080
aacgccctcg ccgaggtgct tcggttcggc gaccgggctt tctacgatga ctggtggaac 1140
agcgagagcc taggcgccta ctggcggacc tggaacaagc ccgtctacac ctacttcaag 1200
cgccacgtgt acatgcccat gatcgggcgt ggctggagtc ccagggtggc cagtctggtc 1260
gtcttcttca tctcagccgt cctccacgag atccttgtcg ggctacccac tcacaacgtc 1320
atcggcgtcg cctttctcgg catgtttctc cagctgcctc tcatcgccat cacggcgccc 1380
atggagaaga tgaggctcgg caaaggcggc aagctcgtag gcaacgtcat cttctgggtg 1440
tcgtttacca tctttggcca gccctttgcg acattgatgt acttttatgc ttggcaggcc 1500
aaatacggga gcgtgagcag ggagatgcag caagcggcaa gcatcaagta a 1551
<210> 149
<211> 510
<212> PRT
<213> 绿木霉
<400> 149
Met Ala Pro Pro Ala Glu Ser Ser Thr Thr Thr Ser Val Glu Ala Ser
1 5 10 15
Thr Gly Ser Val Ser Arg Arg His Ala Ser Gln Ser Glu Ala Asp Leu
20 25 30
Thr Ser Val Glu Pro Val Asn Gly Thr Thr Lys Asn Arg Leu Ser Lys
35 40 45
Thr Pro Pro Lys Lys Tyr Arg His Val Ala Ala Val His Ser Gln Thr
50 55 60
Arg Pro Ser Cys Leu Ser His Asp Ser Pro Ala Ala Pro Ser Phe Leu
65 70 75 80
Gly Phe Arg Asn Leu Met Val Ile Val Leu Val Val Gly Asn Leu Arg
85 90 95
Leu Met Ile Glu Asn Ile Gln Lys Tyr Gly Val Leu Ile Cys Ile Arg
100 105 110
Cys His Asp Tyr Arg Arg Gln Asp Val Leu Leu Gly Leu Leu Leu Tyr
115 120 125
Phe Leu Ile Pro Cys His Leu Phe Ala Ala Tyr Leu Ile Glu Leu Val
130 135 140
Ala Ala Lys Gln Ala Glu Gly Ser Arg Lys Arg Ile Lys Asp Asn Asn
145 150 155 160
Ser Gly Pro Ser Glu Ala Glu Arg Lys Lys Phe His Ser Ile Trp Val
165 170 175
Leu Ala Ala Leu Ala His Gly Ile Asn Ile Thr Leu Ala Leu Ala Ile
180 185 190
Thr Thr Val Val Val Tyr Phe Tyr Val Tyr His Pro Leu Ile Gly Thr
195 200 205
Leu Thr Glu Met His Ala Ile Ile Val Trp Leu Lys Thr Ala Ser Tyr
210 215 220
Ala Phe Thr Asn Arg Asp Leu Arg His Ala Tyr Leu His Pro Val Glu
225 230 235 240
Gly Glu Glu Val Pro Asp Leu Tyr Lys Ser Cys Pro Tyr Pro Gln Asn
245 250 255
Val Thr Met Lys Asn Leu Val Tyr Phe Trp Trp Ala Pro Thr Leu Val
260 265 270
Tyr Gln Pro Val Tyr Pro Arg Thr Asp Lys Ile Arg Trp Val Phe Val
275 280 285
Phe Lys Arg Leu Gly Glu Ile Phe Cys Leu Ala Val Phe Ile Trp Val
290 295 300
Ala Ser Ala Gln Tyr Ala Thr Pro Val Leu Arg Asn Ser Leu Asp Lys
305 310 315 320
Ile Ala Ser Leu Asp Leu Pro Asn Ile Leu Glu Arg Leu Met Lys Leu
325 330 335
Ser Thr Ile Ser Leu Val Ile Trp Leu Ala Gly Phe Phe Ala Leu Phe
340 345 350
Gln Ser Phe Leu Asn Ala Leu Ala Glu Ile Met Arg Phe Gly Asp Arg
355 360 365
Ser Phe Tyr Asp Asp Trp Trp Asn Ser Glu Ser Leu Gly Ala Tyr Trp
370 375 380
Arg Thr Trp Asn Lys Pro Val Tyr Thr Tyr Phe Lys Arg His Val Tyr
385 390 395 400
Met Pro Met Ile Gly Arg Gly Trp Ser Pro Ala Ala Ala Ser Phe Ala
405 410 415
Val Phe Phe Val Ser Ala Val Leu His Glu Ile Leu Val Gly Val Pro
420 425 430
Thr His Asn Ile Ile Gly Val Ala Phe Phe Gly Met Phe Leu Gln Leu
435 440 445
Pro Leu Ile Ala Ile Thr Thr Pro Leu Glu Lys Met Lys Leu Gly His
450 455 460
Gly Gly Arg Ile Leu Gly Asn Val Ile Phe Trp Val Ser Phe Thr Ile
465 470 475 480
Phe Gly Gln Pro Phe Ala Ala Leu Met Tyr Phe Tyr Ala Trp Gln Ala
485 490 495
Lys Tyr Gly Ser Val Ser Arg Leu Pro Gln Met Val His His
500 505 510
<210> 150
<211> 1533
<212> DNA
<213> 绿木霉
<400> 150
atggcgcctc ctgcagagtc ctccacgacg acaagcgtcg aggcctctac cggctccgtg 60
tctcgccgcc acgcctcaca aagtgaagca gatctaacgt cggtggagcc cgtcaacggc 120
acgaccaaga accggctctc caagacaccg ccgaagaaat atcgccatgt cgctgcggtg 180
cattcccaga cgcggccgtc gtgcctgagc catgattccc ctgcggctcc cagctttctc 240
ggattccgca atctcatggt cattgtgctg gttgttggca atctccgatt gatgattgag 300
aatattcaaa agtacggcgt cttaatttgc atcaggtgtc acgactacag acgtcaagat 360
gtgctcttgg gtcttttgct ttattttctt atcccctgcc atttgtttgc agcatacctg 420
atagagctgg tcgctgccaa gcaggctgag ggatccagga agcgaatcaa ggacaacaac 480
tctggcccgt cagaggcaga gcgcaagaag ttccactcaa tctgggttct tgcggctttg 540
gcccatggaa tcaacatcac tcttgccctt gcaattacca ccgttgtggt ctacttttac 600
gtctatcatc cgctgattgg cactttgacc gagatgcatg ccatcattgt gtggctcaag 660
acggcatcat atgcattcac caaccgagat cttcgtcacg cctatctgca tccagttgag 720
ggagaggaag tgcctgattt gtacaaatcc tgcccctatc cacaaaacgt gacgatgaag 780
aacttggtat acttctggtg ggctccgact ctggtgtacc aacctgttta tccgcggacc 840
gacaagattc gatgggtgtt cgtgtttaag cgactaggag agatcttttg ccttgctgtg 900
ttcatttggg ttgccagtgc ccaatatgcc acccccgttt tgcgcaactc tctcgacaag 960
attgcctctc ttgatttgcc caacatcttg gagcggctta tgaaactctc gacaatctct 1020
ttggtcatct ggctggccgg cttctttgcg ctcttccaat ctttcttaaa cgcccttgcc 1080
gagataatga ggtttggcga taggtcattc tacgacgact ggtggaacag tgagagcttg 1140
ggcgcctact ggaggacgtg gaacaagcct gtttatactt acttcaagcg ccatgtctat 1200
atgcccatga tcggacgagg ctggagcccg gccgctgcca gtttcgcagt cttttttgtt 1260
tctgccgttc ttcatgaaat tcttgttggt gttccaacac ataacattat cggcgtcgct 1320
ttcttcggca tgttccttca gcttcctctc atcgccatta ctactccgct ggagaagatg 1380
aaactcggtc atggtggccg cattcttgga aatgtcatat tttgggtttc gtttacaatc 1440
tttggacagc cattcgcggc cctgatgtat ttctacgctt ggcaggccaa gtatggcagc 1500
gtgagtaggt tacctcagat ggtgcaccac taa 1533
<210> 151
<211> 332
<212> PRT
<213> 蓖麻
<400> 151
Met Glu Val Ser Gly Leu Gly Cys Phe Ser Ser Ala Ala Thr Pro Ser
1 5 10 15
Leu Cys Gly Ala Val Asp Ser Gly Gly Val Ser Ser Leu Arg Pro Arg
20 25 30
Lys Ala Phe His Arg Val Ser Asp Ser Cys Leu Gly Phe Arg Asp Asn
35 40 45
Gly His Leu Gln Tyr Tyr Cys Gln Gly Gly Phe Val Arg Cys Gly Gly
50 55 60
Gly Asn Lys Lys Ser Ile Lys Lys Lys Leu Lys Leu Val Lys Ser Leu
65 70 75 80
Ser Glu Asp Phe Ser Met Phe Pro His Asn Asn Ala Leu Leu His Gln
85 90 95
Pro Gln Ser Ile Ser Leu Gln Glu Ala Ala Gln Gly Leu Met Lys Gln
100 105 110
Leu Gln Glu Leu Arg Ala Lys Glu Lys Glu Leu Lys Arg Gln Lys Lys
115 120 125
Gln Glu Lys Lys Ala Lys Leu Lys Ser Glu Ser Ser Ser Ser Ser Ser
130 135 140
Ser Glu Ser Ser Ser Asp Ser Glu Arg Gly Glu Val Ile His Met Ser
145 150 155 160
Arg Phe Arg Asp Glu Thr Ile Pro Ala Ala Leu Pro Gln Leu His Pro
165 170 175
Leu Thr His His His Pro Thr Ser Thr Leu Pro Val Ser Pro Thr Gln
180 185 190
Glu Cys Asn Pro Met Asp Tyr Thr Ser Thr His His Glu Lys Arg Cys
195 200 205
Cys Val Gly Pro Ser Thr Gly Ala Asp Asn Ala Val Gly Asp Cys Cys
210 215 220
Asn Asp Arg Asn Ser Ser Met Thr Glu Glu Leu Ser Ala Asn Arg Ile
225 230 235 240
Glu Val Cys Met Gly Asn Lys Cys Lys Lys Ser Gly Gly Ala Ala Leu
245 250 255
Leu Glu Glu Phe Gln Arg Val Leu Gly Val Glu Ala Ala Val Val Gly
260 265 270
Cys Lys Cys Met Gly Asn Cys Arg Asp Gly Pro Asn Val Arg Val Arg
275 280 285
Asn Ser Val Gln Asp Arg Asn Thr Asp Asp Ser Val Arg Thr Pro Ser
290 295 300
Asn Pro Leu Cys Ile Gly Val Gly Leu Glu Asp Val Asp Val Ile Val
305 310 315 320
Ala Asn Phe Phe Gly Leu Gly Leu Ala Pro Ala Ser
325 330
<210> 152
<211> 999
<212> DNA
<213> 蓖麻
<400> 152
atggaagtct caggcctggg ctgcttctcc tcggctgcaa cgccatcttt gtgtggggcg 60
gtggattcag gcggagtatc ctctttgaga ccgaggaagg cattccatag ggtttctgat 120
tcttgtttag ggtttagaga taatggacat ctgcagtatt attgtcaagg aggatttgtc 180
aggtgcggag gagggaacaa gaaatctatc aagaaaaagt tgaaattagt gaagtccttg 240
tctgaggact tttccatgtt tcctcataac aatgctttgc tccatcaacc tcaatccatc 300
tccctccagg aagctgcaca aggattaatg aaacagctcc aagaattgcg agcaaaggag 360
aaggaattaa agaggcagaa gaaacaagag aaaaaagcca agctaaaatc tgaatcatcc 420
tcatcctcat cctctgaatc cagtagtgat agcgaacgtg gggaggttat tcacatgagc 480
cgcttcagag atgaaactat tcctgccgca ctacctcaat tgcacccact tactcatcac 540
cacccaactt ccaccctacc agtctcccca acccaagaat gcaacccgat ggattacact 600
tcaacacatc atgaaaaacg atgctgcgtt ggaccaagca ccggtgccga taacgcagtc 660
ggtgactgtt gcaatgatag gaatagctcg atgacagagg aattgtcagc aaacagaatt 720
gaggtgtgca tgggtaataa gtgcaagaag tcgggaggtg cagcgttatt ggaggaattt 780
cagagggttt tgggtgtaga ggctgcagtt gttgggtgca agtgcatggg gaactgcagg 840
gacggtccta atgtaagggt caggaattct gtccaagaca gaaacacaga tgactctgtt 900
cgaaccccct ccaatcctct ctgcattggt gttggtttgg aggatgtgga tgttattgtg 960
gccaatttct ttgggttggg tctggcccct gcatcttaa 999
<210> 153
<211> 345
<212> PRT
<213> 花生
<400> 153
Met Glu Val Ser Gly Ala Val Leu Arg Asn Val Thr Cys Pro Ser Phe
1 5 10 15
Ser Val His Val Ser Ser Arg Arg Arg Gly Gly Asp Ser Cys Val Thr
20 25 30
Val Pro Val Arg Met Arg Lys Lys Ala Val Val Arg Cys Cys Cys Gly
35 40 45
Phe Ser Asp Ser Gly His Val Gln Tyr Tyr Gly Asp Glu Lys Lys Lys
50 55 60
Glu Asn Gly Thr Ala Met Leu Ser Thr Lys Lys Lys Leu Lys Met Leu
65 70 75 80
Lys Lys Arg Val Leu Phe Asp Asp Leu Gln Gly Asn Leu Thr Trp Asp
85 90 95
Ala Ala Met Val Leu Met Lys Gln Leu Glu Gln Val Arg Ala Glu Glu
100 105 110
Lys Glu Leu Lys Lys Lys Arg Lys Gln Glu Lys Lys Glu Ala Lys Leu
115 120 125
Lys Ala Ser Lys Met Asn Thr Asn Pro Asp Cys Glu Ser Ser Ser Ser
130 135 140
Ser Ser Ser Ser Glu Ser Glu Ser Glu Ser Ser Glu Ser Glu Cys Asp
145 150 155 160
Asn Glu Val Val Asp Met Lys Lys Asn Ile Lys Val Gly Val Ala Val
165 170 175
Ala Val Ala Asp Ser Pro Arg Lys Ala Glu Thr Met Ile Leu Tyr Thr
180 185 190
Ser Leu Val Ala Arg Asp Val Ser Ala Asn His His His His Asn Ala
195 200 205
Val Glu Leu Phe Ser Arg Asn Asn Asp Ile Ser Val Gly Ser Ile Asn
210 215 220
Gly Gly Leu Lys Asn Glu Asn Thr Ala Val Ile Thr Thr Glu Ala Ile
225 230 235 240
Pro Gln Lys Arg Ile Glu Val Cys Met Gly Asn Lys Cys Lys Lys Ser
245 250 255
Gly Ser Ile Ala Leu Leu Gln Glu Phe Glu Arg Val Val Gly Ala Glu
260 265 270
Gly Gly Ala Ala Ala Ala Val Val Gly Cys Lys Cys Met Gly Lys Cys
275 280 285
Lys Ser Ala Pro Asn Val Arg Ile Gln Asn Ser Thr Ala Asp Lys Ile
290 295 300
Ala Glu Gly Phe Asn Asp Ser Val Lys Val Pro Ala Asn Pro Leu Cys
305 310 315 320
Ile Gly Val Ala Trp Arg Met Leu Lys Pro Leu Trp Leu Arg Phe Leu
325 330 335
Gly Glu Asn Gln Glu Ser Thr Asn Glu
340 345
<210> 154
<211> 1038
<212> DNA
<213> 花生
<400> 154
atggaggttt caggcgccgt tctaaggaat gtcacgtgcc cttccttttc tgtgcacgtg 60
agttcccgtc gtcgtggtgg tgatagttgt gttacagtgc cggtgaggat gagaaaaaag 120
gcggtggtgc gttgttgctg cgggttcagt gattcggggc atgtgcagta ttacggggac 180
gagaagaaga aggagaatgg aaccgctatg ttgagcacca agaagaagct caagatgctg 240
aagaaacgtg tccttttcga tgatcttcaa ggaaacctga cttgggatgc tgctatggtt 300
ttgatgaagc agctagagca agtaagggca gaggagaagg aattgaagaa aaaaaggaag 360
caagagaaga aggaggcaaa actcaaagcc tctaagatga acaccaatcc tgattgcgaa 420
tcgtcatcgt catcgtcatc atctgaatct gaatctgaat caagtgagag tgaatgtgac 480
aatgaggtgg ttgacatgaa gaagaacatt aaggttggtg ttgccgttgc tgttgccgat 540
tccccacgaa aggcggaaac catgattcta tacacctccc ttgttgcccg agatgttagt 600
gctaatcatc atcatcataa tgccgtggaa ttattctcta gaaacaatga catatcagtt 660
ggaagcatta atggtggcct taagaatgag aatactgcgg ttattaccac tgaagctatt 720
cctcagaaga ggattgaggt atgcatggga aacaagtgca agaaatccgg atctattgca 780
ttgttgcaag aatttgagag agtggttggt gctgaaggag gtgctgctgc tgcagttgtt 840
ggatgcaagt gcatggggaa gtgcaagagt gcacctaatg tgaggattca gaactctact 900
gcagataaaa tagctgaggg gttcaatgat tcagttaagg ttccagctaa ccctctttgc 960
attggggttg catggaggat gttgaaacca ttgtggctta gattcttggg cgagaatcag 1020
gaaagtacta atgaataa 1038
<210> 155
<211> 267
<212> PRT
<213> 褐家鼠
<400> 155
Met Asn Met Ser Val Leu Thr Leu Gln Glu Tyr Glu Phe Glu Lys Gln
1 5 10 15
Phe Asn Glu Asn Glu Ala Ile Gln Trp Met Gln Glu Asn Trp Lys Lys
20 25 30
Ser Phe Leu Phe Ser Ala Leu Tyr Ala Ala Phe Ile Phe Gly Gly Arg
35 40 45
His Leu Met Asn Lys Arg Ala Lys Phe Glu Leu Arg Lys Pro Leu Val
50 55 60
Leu Trp Ser Leu Thr Leu Ala Val Phe Ser Ile Phe Gly Ala Leu Arg
65 70 75 80
Thr Gly Ala Tyr Met Leu Tyr Ile Leu Met Thr Lys Gly Leu Lys Gln
85 90 95
Ser Val Cys Asp Gln Ser Phe Tyr Asn Gly Pro Val Ser Lys Phe Trp
100 105 110
Ala Tyr Ala Phe Val Leu Ser Lys Ala Pro Glu Leu Gly Asp Thr Ile
115 120 125
Phe Ile Ile Leu Arg Lys Gln Lys Leu Ile Phe Leu His Trp Tyr His
130 135 140
His Ile Thr Val Leu Leu Tyr Ser Trp Tyr Ser Tyr Lys Asp Met Val
145 150 155 160
Ala Gly Gly Gly Trp Phe Met Thr Met Asn Tyr Gly Val His Ala Val
165 170 175
Met Tyr Ser Tyr Tyr Ala Leu Arg Ala Ala Gly Phe Arg Val Ser Arg
180 185 190
Lys Phe Ala Met Phe Ile Thr Leu Ser Gln Ile Thr Gln Met Leu Met
195 200 205
Gly Cys Val Ile Asn Tyr Leu Val Phe Asn Trp Met Gln His Asp Asn
210 215 220
Asp Gln Cys Tyr Ser His Phe Gln Asn Ile Phe Trp Ser Ser Leu Met
225 230 235 240
Tyr Leu Ser Tyr Leu Leu Leu Phe Cys His Phe Phe Phe Glu Ala Tyr
245 250 255
Ile Gly Lys Val Lys Lys Ala Thr Lys Ala Glu
260 265
<210> 156
<211> 804
<212> DNA
<213> 褐家鼠
<400> 156
atgaacatgt cagtgttgac tttacaagaa tatgaattcg agaagcagtt caacgagaat 60
gaagccatcc aatggatgca ggaaaactgg aagaaatctt tcctgttttc tgcgctgtac 120
gctgccttta tatttggtgg tcggcatctg atgaacaagc gggccaagtt tgaactgcgg 180
aagccgctcg tgctctggtc actgactctt gcggtcttca gtatattcgg tgctcttcga 240
actggtgctt acatgctgta cattttgatg accaaaggcc tgaagcagtc agtttgtgac 300
cagagttttt acaacggacc tgtcagcaaa ttctgggctt acgcgtttgt gctcagcaaa 360
gcacccgaac taggtgatac gatattcatc attctgagga agcagaagct gatcttcctg 420
cactggtacc accacatcac tgtgctcctg tactcttggt actcctacaa agacatggta 480
gctgggggtg gttggttcat gactatgaac tatggcgtac acgccgtcat gtactcttac 540
tacgccttgc gggctgcggg tttccgggtc tcccggaagt ttgccatgtt catcacgttg 600
tcccagatca ctcagatgct gatgggctgt gtcattaact acctggtctt caactggatg 660
cagcatgaca atgaccagtg ctactcccac tttcagaaca tcttctggtc ctcactcatg 720
tacctcagct accttctgct cttctgccat ttcttctttg aggcctacat cggcaaagtg 780
aagaaagcga cgaaggccga gtag 804
<210> 157
<211> 206
<212> PRT
<213> 酿酒酵母
<400> 157
Met Val Ser Asp Trp Lys Asn Cys Lys Ala Ser Arg Arg Thr Asp Arg
1 5 10 15
Asn Tyr Trp Asp Tyr Asn Arg Ala Val Gly Trp Ala Thr Ala Gly Arg
20 25 30
Lys Asp Thr Val Gly Lys Ser Arg Val Ala Met Tyr Tyr Val Val Gly
35 40 45
Gly Arg Ser Val Lys Ser Cys Lys Lys Arg Ser Val His Asn Met Thr
50 55 60
Ser Val Ser Trp Met Val Met Val Tyr Arg His Gly Tyr Ala Val Cys
65 70 75 80
Asn Val Ser Trp Thr Met Thr Tyr Tyr Asn Tyr Met Thr Lys Val Ala
85 90 95
Asp Thr Val Met Val Lys His Arg Lys Thr His Thr Tyr His His Gly
100 105 110
Ala Thr Ala Cys Tyr Asn Val Gly Tyr Thr Ala Val Thr Trp Val Val
115 120 125
Thr Asn Ala Val His Val Met Tyr Trp Tyr Tyr Ser Ala Ser Gly Arg
130 135 140
Val Trp Trp Lys Ala Trp Val Thr Arg Val Met Asp Val Val Tyr Tyr
145 150 155 160
Val Tyr Lys Val Ala Ala Tyr Lys Asn Ala Cys Thr Cys Asp Cys Gly
165 170 175
Ser Met Thr Ala Ala Ala Gly Ala Ala Thr Ser Tyr Ser Tyr Val Tyr
180 185 190
Lys Arg Gly Ser Ala Ser Gly Lys Lys Lys Asn Lys Asn Asn
195 200 205
<210> 158
<211> 933
<212> DNA
<213> 酿酒酵母
<400> 158
atggtaagtg attggaaaaa tttttgcctc gagaaagcct ctaggtttcg cccaacaata 60
gataggcctt tttttaatat ttatttgtgg gactatttca atcgtgcggt tgggtgggcc 120
actgcaggtc gcttccagcc aaaggatttt gagtttaccg ttgggaagca gcctttgagt 180
gaacctcgtc cggtactgct ttttattgcc atgtattatg tggttatatt tggcgggagg 240
tccctggtaa agtcatgtaa acctctcaag ttgagattta tttctcaagt ccataacttg 300
atgttgactt ctgtttcctt tttatggttg attttgatgg tggaacagat gctacccata 360
gtgtatcgcc atgggctgta ttttgctgtt tgtaatgttg aatcgtggac gcaaccgatg 420
gagacattat attatctcaa ctatatgaca aagtttgtgg agttcgcaga cactgtcttg 480
atggtgttga aacatagaaa gttgactttc ctacatacat accatcatgg tgctacagct 540
ttactgtgct ataatcaatt ggttggttac acagcagtta catgggttcc tgtcacctta 600
aacttagctg ttcacgttct tatgtattgg tattatttcc tttctgctag cggaattcgt 660
gtttggtgga aagcctgggt tacaagacta caaattgtgc agttcatgct tgatctcatt 720
gtcgtttatt acgtgcttta tcagaagatt gttgctgcat atttcaaaaa tgcttgtact 780
ccacagtgtg aggattgctt aggttcaatg acggctattg ctgctggtgc agccattctt 840
acatcctact tgtttttgtt catctctttc tatattgagg tttacaaacg tggaagtgct 900
agtggtaaga agaagatcaa caaaaacaat taa 933
<210> 159
<211> 334
<212> PRT
<213> 噬腺嘌呤阿克苏拉酵母
<400> 159
Met Leu Glu Val Thr Phe Pro Pro Thr Leu Asp Arg Pro Phe Gly Val
1 5 10 15
Tyr Leu Tyr Gly Leu Phe Asp Ala Leu Thr Asn Gly Trp Ala Thr Arg
20 25 30
Phe Gln Phe Ala Gln Asp Ser Gly Ile Pro Phe Ser Ser Arg Trp Glu
35 40 45
Val Ala Ala Gly Ile Val Thr Tyr Tyr Val Val Ile Phe Gly Gly Arg
50 55 60
Glu Val Leu Lys Asn Ala Pro Val Ile Arg Leu Asn Phe Val Phe Gln
65 70 75 80
Ile His Asn Leu Ile Leu Thr Leu Leu Ser Leu Gly Leu Leu Leu Leu
85 90 95
Leu Val Glu Gln Leu Ile Pro Ile Ile Val Arg His Gly Val Leu Tyr
100 105 110
Ala Ile Cys Asn Ser Gly Ser Trp Thr Gln Pro Ile Val Thr Val Tyr
115 120 125
Tyr Leu Asn Tyr Leu Thr Lys Tyr Tyr Glu Leu Phe Asp Thr Val Phe
130 135 140
Leu Val Leu Arg Lys Lys Pro Leu Thr Phe Leu His Thr Tyr His His
145 150 155 160
Gly Ala Thr Ala Leu Leu Cys Phe Thr Gln Leu Ile Gly His Thr Ser
165 170 175
Val Ser Trp Val Pro Ile Val Leu Asn Leu Phe Val His Val Ile Met
180 185 190
Tyr Tyr Tyr Tyr Phe Leu Ser Ala Leu Gly Val Arg Asn Ile Trp Trp
195 200 205
Lys Glu Trp Val Thr Arg Thr Gln Ile Ile Gln Phe Val Val Asp Leu
210 215 220
Val Phe Val Tyr Phe Ala Thr Tyr Thr Tyr Phe Thr Asn Lys Tyr Trp
225 230 235 240
Pro Trp Leu Pro Asn Lys Gly Thr Cys Ala Gly Glu Glu Phe Ala Ala
245 250 255
Ile Tyr Gly Cys Ala Leu Leu Thr Ser Tyr Leu Phe Leu Phe Ile Ala
260 265 270
Phe Tyr Ile Arg Val Tyr Thr Lys Ala Lys Ala Lys Gly Arg Lys Arg
275 280 285
Ala Ala Ser Ala Ala Ala Lys Ala Thr Thr Gly Val Val Thr Ala Asp
290 295 300
Arg Pro Ser Thr Pro Ile Ala Thr Thr Asn Gly Ala Ala Thr Gly Ala
305 310 315 320
Ala Gly Ala Thr Gly Ser Val Lys Ser Arg Ser Arg Lys Ala
325 330
<210> 160
<211> 1005
<212> DNA
<213> 噬腺嘌呤阿克苏拉酵母
<400> 160
atgctggaag tcaccttccc cccgaccctg gaccgcccct ttggcgtgta cttgtacggg 60
ctgtttgacg cgttgaccaa cggatgggca acccgattcc agtttgctca ggactctggc 120
atccccttta gctcccgctg ggaagtggcc gcgggcattg tcacctacta cgtggtcatc 180
tttggcggcc gcgaggtgct caagaatgcc ccggtgattc gtctcaactt tgtgttccag 240
attcacaacc ttattctgac cctgctgtcc ctgggtctgt tgctgctgct ggtggagcag 300
ttgattccta tcattgtgcg ccacggagtg ctgtacgcca tctgcaactc tggatcgtgg 360
actcagccta ttgtgaccgt gtactacctc aactacctga caaagtacta cgagctgttt 420
gacactgtgt tcttggtgct gcgaaagaag cctctgacct tcttgcacac ttaccaccac 480
ggagccaccg ctctgctgtg cttcacccag ctcattggcc acacctcggt gtcgtgggtg 540
cccattgtgc tcaacctgtt tgtccatgtt atcatgtact actactactt tttgagcgct 600
ctgggagttc gcaacatctg gtggaaggag tgggtcactc gaacccagat catccagttt 660
gtggtggacc tggtgtttgt gtactttgct acctacacct actttaccaa caagtactgg 720
ccctggctcc ctaacaaggg cacttgcgct ggtgaggagt ttgctgccat ctacggatgt 780
gccctgctca cttcgtacct gttcctgttc attgcctttt acattcgcgt gtacaccaag 840
gcaaaggcca agggtcgcaa gagagctgcc agcgctgctg ccaaggccac cactggcgtt 900
gtcactgccg accgtccttc cactccaatt gctaccacca acggagctgc cactggcgct 960
gctggagcca ctggttcggt caagtctcga tcgcgcaagg cctaa 1005
<210> 161
<211> 341
<212> PRT
<213> 大肠杆菌
<400> 161
Met Lys Lys Pro Glu Leu Thr Ala Thr Ser Val Glu Lys Phe Leu Ile
1 5 10 15
Glu Lys Phe Asp Ser Val Ser Asp Leu Met Gln Leu Ser Glu Gly Glu
20 25 30
Glu Ser Arg Ala Phe Ser Phe Asp Val Gly Gly Arg Gly Tyr Val Leu
35 40 45
Arg Val Asn Ser Cys Ala Asp Gly Phe Tyr Lys Asp Arg Tyr Val Tyr
50 55 60
Arg His Phe Ala Ser Ala Ala Leu Pro Ile Pro Glu Val Leu Asp Ile
65 70 75 80
Gly Glu Phe Ser Glu Ser Leu Thr Tyr Cys Ile Ser Arg Arg Ala Gln
85 90 95
Gly Val Thr Leu Gln Asp Leu Pro Glu Thr Glu Leu Pro Ala Val Leu
100 105 110
Gln Pro Val Ala Glu Ala Met Asp Ala Ile Ala Ala Ala Asp Leu Ser
115 120 125
Gln Thr Ser Gly Phe Gly Pro Phe Gly Pro Gln Gly Ile Gly Gln Tyr
130 135 140
Thr Thr Trp Arg Asp Phe Ile Cys Ala Ile Ala Asp Pro His Val Tyr
145 150 155 160
His Trp Gln Thr Val Met Asp Asp Thr Val Ser Ala Ser Val Ala Gln
165 170 175
Ala Leu Asp Glu Leu Met Leu Trp Ala Glu Asp Cys Pro Glu Val Arg
180 185 190
His Leu Val His Ala Asp Phe Gly Ser Asn Asn Val Leu Thr Asp Asn
195 200 205
Gly Arg Ile Thr Ala Val Ile Asp Trp Ser Glu Ala Met Phe Gly Asp
210 215 220
Ser Gln Tyr Glu Val Ala Asn Ile Phe Phe Trp Arg Pro Trp Leu Ala
225 230 235 240
Cys Met Glu Gln Gln Thr Arg Tyr Phe Glu Arg Arg His Pro Glu Leu
245 250 255
Ala Gly Ser Pro Arg Leu Arg Ala Tyr Met Leu Arg Ile Gly Leu Asp
260 265 270
Gln Leu Tyr Gln Ser Leu Val Asp Gly Asn Phe Asp Asp Ala Ala Trp
275 280 285
Ala Gln Gly Arg Cys Asp Ala Ile Val Arg Ser Gly Ala Gly Thr Val
290 295 300
Gly Arg Thr Gln Ile Ala Arg Arg Ser Ala Ala Val Trp Thr Asp Gly
305 310 315 320
Cys Val Glu Val Leu Ala Asp Ser Gly Asn Arg Arg Pro Ser Thr Arg
325 330 335
Pro Arg Ala Lys Glu
340
<210> 162
<211> 1026
<212> DNA
<213> 大肠杆菌
<400> 162
atgaagaagc ccgagctgac cgctacctct gttgagaagt tcctgattga gaagtttgat 60
tccgtttccg acctgatgca gctgtccgag ggcgaggagt ctcgagcctt ctcctttgac 120
gtgggcggac gaggttacgt tctgcgagtg aactcgtgtg ccgacggctt ctacaaggat 180
cgatacgtct accgacactt tgcttctgcc gctctgccca tccctgaggt tctcgacatt 240
ggcgagttct ctgagtccct cacctactgc atctctcgac gagctcaggg agtcaccctg 300
caggacctcc ctgagactga gctgcctgct gtcctccagc ctgttgctga ggccatggac 360
gctatcgctg ctgctgatct gtcccagacc tcgggtttcg gcccctttgg acctcaggga 420
attggacagt acaccacttg gcgagacttc atctgtgcta ttgccgatcc tcacgtctac 480
cattggcaga ccgttatgga cgatactgtg tcggcttctg tcgctcaggc tctggacgag 540
ctgatgctct gggccgagga ttgccccgag gttcgacacc tggtgcatgc tgacttcggt 600
tccaacaacg ttctcaccga caacggccga atcactgccg tgattgactg gtccgaggct 660
atgtttggcg actcgcagta cgaggtggcc aacatcttct tttggcgacc ctggctggct 720
tgtatggagc agcagacccg atacttcgag cgacgacatc ctgagctcgc tggatcccct 780
cgactgcgag cttacatgct ccgaattggt ctggaccagc tctaccagtc gctggtggat 840
ggcaactttg acgatgctgc ctgggctcag ggacgatgtg acgccatcgt gcgatctggc 900
gctggaaccg tcggacgaac tcagattgcc cgacgatccg ctgctgtctg gaccgacgga 960
tgcgtggagg tcctggctga ttcgggtaac cgacgaccct ctactcgacc tcgagctaag 1020
gagtaa 1026
<210> 163
<211> 24
<212> DNA
<213> 人工序列
<220>
<223> 人工序列的说明：合成引物
<400> 163
accacttggc gagacttcat ctgt 24
<210> 164
<211> 24
<212> DNA
<213> 人工序列
<220>
<223> 人工序列的说明：合成引物
<400> 164
agcatcgtca aagttgccat ccac 24
<210> 165
<211> 60
<212> DNA
<213> 人工序列
<220>
<223> 人工序列的说明：合成引物
<400> 165
cagctctctt cccccgttca gctccttttc taccgcgatt atgaagaagc ccgagctgac 60
<210> 166
<211> 60
<212> DNA
<213> 人工序列
<220>
<223> 人工序列的说明：合成引物
<400> 166
tttagtctca tcgttagtag ttatgtgctc tgctcggggt tactccttag ctcgaggtcg 60
<210> 167
<211> 898
<212> DNA
<213> 人工序列
<220>
<223> 人工序列的说明：合成多核苷酸
<400> 167
cagctctctt cccccgttca gctccttttc taccgcgatt atgaagaagc ccgagctgac 60
cgctacctct gttgagaagt tcctgattga gaagtttgat tccgtttccg acctgatgca 120
gctgtccgag ggcgaggagt ctcgagcctt ctcctttgac gtgggcggac gaggttacgt 180
tctgcgagtg aactcgtgtg ccgacggctt ctacaaggat cgatacgtct accgacactt 240
tgcttctgcc gctctgccca tccctgaggt tctcgacatt ggcgagttct ctgagtccct 300
cacctactgc atctctcgac gagctcaggg agtcaccctg caggacctcc ctgagactga 360
gctgcctgct gtcctccagc ctgttgctga ggccatggac gctatcgctg ctgctgatct 420
gtcccagacc tcgggtttcg gcccctttgg acctcaggga attggacagt acaccacttg 480
gcgagacttc atctgtgcta ttgccgatcc tcacgtctac cattggcaga ccgttatgga 540
cgatactgtg tcggcttctg tcgctcaggc tctggacgag ctgatgctct gggccgagga 600
ttgccccgag gttcgacacc tggtgcatgc tgacttcggt tccaacaacg ttctcaccga 660
caacggccga atcactgccg tgattgactg gtccgaggct atgtttggcg actcgcagta 720
cgaggtggcc aacatcttct tttggcgacc ctggctggct tgtatggagc agcagacccg 780
atacttcgag cgacgacatc ctgagctcgc tggatcccct cgactgcgag cttacatgct 840
ccgaattggt ctggaccagc tctaccagtc gctggtggat ggcaactttg acgatgct 898
<210> 168
<211> 633
<212> DNA
<213> 人工序列
<220>
<223> 人工序列的说明：合成多核苷酸
<400> 168
accacttggc gagacttcat ctgtgctatt gccgatcctc acgtctacca ttggcagacc 60
gttatggacg atactgtgtc ggcttctgtc gctcaggctc tggacgagct gatgctctgg 120
gccgaggatt gccccgaggt tcgacacctg gtgcatgctg acttcggttc caacaacgtt 180
ctcaccgaca acggccgaat cactgccgtg attgactggt ccgaggctat gtttggcgac 240
tcgcagtacg aggtggccaa catcttcttt tggcgaccct ggctggcttg tatggagcag 300
cagacccgat acttcgagcg acgacatcct gagctcgctg gatcccctcg actgcgagct 360
tacatgctcc gaattggtct ggaccagctc taccagtcgc tggtggatgg caactttgac 420
gatgctgcct gggctcaggg acgatgtgac gccatcgtgc gatctggcgc tggaaccgtc 480
ggacgaactc agattgcccg acgatccgct gctgtctgga ccgacggatg cgtggaggtc 540
ctggctgatt cgggtaaccg acgaccctct actcgacctc gagctaagga gtaaccccga 600
gcagagcaca taactactaa cgatgagact aaa 633

Claims (20)

1.一种转化的细胞，所述细胞包含第一遗传修饰和第二遗传修饰，所述第一遗传修饰和所述第二遗传修饰选自由以下各项组成的组：
降低天然甘油-3-磷酸酰基转移酶蛋白、天然Δ9去饱和酶蛋白、或天然Δ12去饱和酶蛋白的活性的遗传修饰；以及
增加甘油-3-磷酸酰基转移酶蛋白、延长酶蛋白、或二酰基甘油酰基转移酶蛋白的表达的遗传修饰。
2.如权利要求1所述的细胞，其中所述第一遗传修饰或所述第二遗传修饰是用包含编码甘油-3-磷酸酰基转移酶蛋白、Δ9去饱和酶蛋白、延长酶蛋白、或二酰基甘油酰基转移酶蛋白的基因的核酸进行转化。
3.如权利要求2所述的细胞，其中所述核酸编码与以下各项中所示的序列具有至少95％序列同源性的氨基酸序列：SEQ ID NO:31；SEQ ID NO:33；SEQ ID NO:35；SEQ ID NO:37；SEQ ID NO:39；SEQ ID NO:93；SEQ ID NO:97；SEQ ID NO:101；SEQ ID NO:103；SEQ IDNO:143；SEQ ID NO:145；SEQ ID NO:147；SEQ ID NO:149；SEQ ID NO:19；SEQ ID NO:21；SEQ ID NO:23；SEQ ID NO:25；SEQ ID NO:27；SEQ ID NO:29；SEQ ID NO:51；SEQ ID NO:95；SEQ ID NO:99；SEQ ID NO:127；SEQ ID NO:129；SEQ ID NO:131；SEQ ID NO:133；SEQID NO:135；SEQ ID NO:137；SEQ ID NO:139；SEQ ID NO:141；SEQ ID NO:151；SEQ ID NO:153；SEQ ID NO:5；SEQ ID NO:107；SEQ ID NO:155；SEQ ID NO:157；SEQ ID NO:159；SEQID NO:17；SEQ ID NO:41；SEQ ID NO:43；SEQ ID NO:45；SEQ ID NO:47；SEQ ID NO:115；或SEQ ID NO:117。
4.如权利要求1至3中任一项所述的细胞，其中所述第一遗传修饰或所述第二遗传修饰(a)降低天然甘油-3-磷酸酰基转移酶蛋白、天然Δ9去饱和酶蛋白、或天然Δ12去饱和酶蛋白的活性，并且(b)所述修饰是敲除突变。
5.如权利要求1至4中任一项所述的细胞，所述细胞进一步包含第三遗传修饰，所述第三遗传修饰选自由以下各项组成的组：
提高以下各项的活性的遗传修饰：Δ9去饱和酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、或磷脂:二酰基甘油酰基转移酶；以及
降低以下各项的活性的遗传修饰：天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、或天然磷脂:二酰基甘油酰基转移酶。
6.如权利要求5所述的细胞，其中：
所述第三遗传修饰提高以下各项的活性：Δ9去饱和酶、sn-2酰基甘油脂肪酰基转移酶、溶血磷脂酸酰基转移酶、磷脂酸磷酸酶、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶、或磷脂:二酰基甘油酰基转移酶；并且
所述第三遗传修饰是用包含编码以下各项的基因的核酸进行转化：Δ9去饱和酶蛋白、sn-2酰基甘油脂肪酰基转移酶蛋白、溶血磷脂酸酰基转移酶蛋白、磷脂酸磷酸酶蛋白、甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶蛋白、或磷脂:二酰基甘油酰基转移酶蛋白。
7.如权利要求6所述的细胞，其中所述核酸编码与以下各项中所示的序列具有至少95％序列同源性的氨基酸序列：SEQ ID NO:3；SEQ ID NO:7；SEQ ID NO:9；SEQ ID NO:11；SEQ ID NO:13；SEQ ID NO:15；SEQ ID NO:111；SEQ ID NO:113；SEQ ID NO:59；SEQ ID NO:61；SEQ ID NO:63；SEQ ID NO:65；SEQ ID NO:67；SEQ ID NO:69；SEQ ID NO:71；SEQ IDNO:73；SEQ ID NO:75；SEQ ID NO:77；SEQ ID NO:79；SEQ ID NO:81；SEQ ID NO:83；SEQ IDNO:53；SEQ ID NO:55；SEQ ID NO:57；SEQ ID NO:119；SEQ ID NO:121；SEQ ID NO:123；SEQID NO:125；SEQ ID NO:105；或SEQ ID NO:109。
8.如权利要求5所述的细胞，其中所述第三遗传修饰是敲除突变。
9.如权利要求5或8所述的细胞，其中：
所述细胞是解脂耶氏酵母(Yarrowia lipolytica)；
所述第三遗传修饰降低以下各项的活性：天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、或天然磷脂:二酰基甘油酰基转移酶；并且
所述天然三酰基甘油脂酶具有SEQ ID NO:91中所示的氨基酸序列；所述天然sn-2酰基甘油脂肪酰基转移酶具有SEQ ID NO:59或SEQ ID NO:65中所示的氨基酸序列；所述天然溶血磷脂酸酰基转移酶具有SEQ ID NO:83中所示的氨基酸序列；所述天然磷脂酸磷酸酶具有SEQ ID NO:57中所示的氨基酸序列；并且所述天然磷脂:二酰基甘油酰基转移酶具有SEQID NO:109中所示的氨基酸序列。
10.如权利要求5或8所述的细胞，其中：
所述细胞是噬腺嘌呤阿克苏拉酵母(Arxula adeninivorans)；
所述第三遗传修饰降低以下各项的活性：天然三酰基甘油脂酶、天然sn-2酰基甘油脂肪酰基转移酶、天然溶血磷脂酸酰基转移酶、天然磷脂酸磷酸酶、或天然磷脂:二酰基甘油酰基转移酶；并且
所述天然三酰基甘油脂酶具有SEQ ID NO:85、SEQ ID NO:87、或SEQ ID NO:89中所示的氨基酸序列；所述天然sn-2酰基甘油脂肪酰基转移酶具有SEQ ID NO:61或SEQ ID NO:63中所示的氨基酸序列；所述天然溶血磷脂酸酰基转移酶具有SEQ ID NO:81中所示的氨基酸序列；所述天然磷脂酸磷酸酶具有SEQ ID NO:55中所示的氨基酸序列；并且所述天然磷脂:二酰基甘油酰基转移酶具有SEQ ID NO:105中所示的氨基酸序列。
11.如权利要求5或8所述的细胞，其中：
所述细胞是酿酒酵母(Saccharomyces cerevisiae)；
所述第三遗传修饰降低天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶的活性；并且
并且所述天然甘油-3-磷酸/二羟丙酮磷酸sn-1酰基转移酶具有SEQ ID NO:119中所示的氨基酸序列。
12.如权利要求1至8中任一项所述的细胞，其中所述细胞选自由以下各项组成的组：藻类、细菌、霉菌、真菌、植物、以及酵母。
13.如权利要求12所述的细胞，其中所述细胞是酵母、真菌、或酵母样藻类。
14.如权利要求13所述的细胞，其中所述细胞选自由以下各项组成的组：阿克苏拉酵母属(Arxula)、曲霉属(Aspegillus)、破囊壶菌属(Aurantiochytrium)、假丝酵母属(Candida)、麦角菌属(Claviceps)、隐球菌属(Cryptococcus)、小克银汉霉菌属(Cunninghamella)、地霉属(Geotrichum)、汉逊酵母属(Hansenula)、克鲁维酵母属(Kluyveromyces)、柯达酵母属(Kodamaea)、白冬孢酵母属(Leucosporidiella)、油脂酵母属(Lipomyces)、被孢霉属(Mortierella)、欧加铁酵母属(Ogataea)、毕赤酵母属(Pichia)、原壁藻属(Prototheca)、根霉属(Rhizopus)、红冬孢酵母属(Rhodosporidium)、红酵母属(Rhodotorula)、酵母菌属(Saccharomyces)、裂殖酵母属(Schizosaccharomyces)、银耳属(Tremella)、丝孢酵母属(Trichosporon)、威克汉姆酵母属(Wickerhamomyces)、以及耶氏酵母属(Yarrowia)。
15.如权利要求14所述的细胞，其中所述细胞选自由以下各项组成的组：噬腺嘌呤阿克苏拉酵母、黑曲霉(Aspergillus niger)、米曲霉(Aspergillus orzyae)、土曲霉(Aspergillus terreus)、裂殖壶菌(Aurantiochytrium limacinum)、产朊假丝酵母(Candida utilis)、紫色麦角菌(Claviceps purpurea)、白色隐球菌(Cryptococcusalbidus)、弯曲隐球菌(Cryptococcus curvatus)、拉氏隐球菌(Cryptococcusramirezgomezianus)、地生隐球菌(Cryptococcus terreus)、威氏隐球菌(Cryptococcuswieringae)、刺孢小克银汉霉菌(Cunninghamella echinulata)、日本小克银汉霉菌(Cunninghamella japonica)、发酵地霉(Geotrichum fermentans)、多形汉逊酵母(Hansenula polymorpha)、乳酸克鲁维酵母(Kluyveromyces lactis)、马克斯克鲁维酵母(Kluyveromyces marxianus)、奥默柯达酵母(Kodamaea ohmeri)、克里替弗拉白冬孢酵母(Leucosporidiella creatinivora)、产油油脂酵母(Lipomyces lipofer)、斯达氏油脂酵母(Lipomyces starkeyi)、子囊菌油脂酵母(Lipomyces tetrasporus)、黄褐色被孢霉(Mortierella isabellina)、高山被孢霉(Mortierella alpina)、多形欧加铁酵母(Ogataea polymorpha)、西弗毕赤酵母(Pichia ciferrii)、季也蒙毕赤酵母(Pichiaguilliermondii)、巴斯德毕赤酵母(Pichia pastoris)、树干毕赤酵母(Pichiastipites)、饶氏原壁藻(Prototheca zopfii)、少根根霉(Rhizopus arrhizus)、巴布维红冬孢酵母(Rhodosporidium babjevae)、圆红冬孢酵母(Rhodosporidium toruloides)、沼泽生红冬孢酵母(Rhodosporidium paludigenum)、粘红酵母(Rhodotorula glutinis)、胶红酵母(Rhodotorula mucilaginosa)、酿酒酵母、粟酒裂殖酵母(Schizosaccharomycespombe)、恩氏银耳(Tremella enchepala)、皮状丝孢酵母(Trichosporon cutaneum)、发酵丝孢酵母(Trichosporon fermentans)、西弗威克汉姆酵母(Wickerhamomyces ciferrii)、以及解脂耶氏酵母。
16.如权利要求15所述的细胞，其中所述细胞选自由以下各项组成的组：噬腺嘌呤阿克苏拉酵母、酿酒酵母、以及解脂耶氏酵母。
17.如权利要求9或16所述的细胞，其中：
所述细胞是解脂耶氏酵母；
所述第一遗传修饰或所述第二遗传修饰降低天然甘油-3-磷酸酰基转移酶蛋白、天然Δ9去饱和酶蛋白、或天然Δ12去饱和酶蛋白的活性；并且
所述天然甘油-3-磷酸酰基转移酶蛋白具有SEQ ID NO:17中所示的氨基酸序列；所述天然Δ9去饱和酶蛋白具有SEQ ID NO:3中所示的氨基酸序列；并且所述天然Δ12去饱和酶蛋白具有SEQ ID NO:1中所示的氨基酸序列。
18.如权利要求10或16所述的细胞，其中：
所述细胞是噬腺嘌呤阿克苏拉酵母；
所述第一遗传修饰或所述第二遗传修饰降低天然甘油-3-磷酸酰基转移酶蛋白、天然Δ9去饱和酶蛋白、或天然Δ12去饱和酶蛋白的活性；并且
所述天然甘油-3-磷酸酰基转移酶蛋白具有SEQ ID NO:43中所示的氨基酸序列；所述天然Δ9去饱和酶蛋白具有SEQ ID NO:7中所示的氨基酸序列；并且所述天然Δ12去饱和酶蛋白具有SEQ ID NO:49中所示的氨基酸序列。
19.如权利要求1至18中任一项所述的细胞，其中所述细胞包含至少50％的脂质，所述50％的脂质是通过干细胞重量％所测量。
20.如权利要求1至19中任一项所述的细胞，其中所述细胞包含浓度为至少70％的油酸，所述油酸的浓度为占所述细胞中总C16和C18脂肪酸的百分比。
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