CN107201229A - A kind of bioremediation agents and its preparation and application with improved soil and regulating and controlling microbial population structure function - Google Patents

A kind of bioremediation agents and its preparation and application with improved soil and regulating and controlling microbial population structure function Download PDF

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Publication number
CN107201229A
CN107201229A CN201710052161.9A CN201710052161A CN107201229A CN 107201229 A CN107201229 A CN 107201229A CN 201710052161 A CN201710052161 A CN 201710052161A CN 107201229 A CN107201229 A CN 107201229A
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soil
bacillus
bioremediation agents
chicken manure
mushroom bran
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CN107201229B (en
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尹淑丽
孙国龙
张飞燕
杨继业
张丽萍
梁然
刘倩倩
刘洪伟
程辉彩
段普凡
崔冠慧
习彦花
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Institute of Biology of Hebei Academy of Sciences
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Institute of Biology of Hebei Academy of Sciences
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    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/40Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01BSOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00Methods for working soil
    • A01B79/02Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00Calcareous fertilisers
    • C05D3/02Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/10Solid or semi-solid fertilisers, e.g. powders
    • C05G5/12Granules or flakes
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2101/00Agricultural use

Abstract

The present invention relates to a kind of microorganism renovation agent for having improved soil and regulating and controlling microbial population structure function, it includes complex function microorganism, biomass carbon, mushroom bran and chicken manure, and its preparation method is:Biomass carbon, mushroom bran and chicken manure are mixed in proportion, complex microorganism dry powder is added, is granulated after mixing through comminutor, the 4mm of particle diameter 2 renovation agent particle is obtained.The use of bioremediation agents of the present invention, can effectively facilitate the growth of crop root, improve the microbial bacteria group structure of soil, soil vitality is activated there is provided the activity of soil enzyme, soil nutrient is obtained Efficient Conversion and utilization, the generation of soil-borne disease is reduced, is easy to the production of green vegetables.

Description

A kind of bioremediation agents with improved soil and regulating and controlling microbial population structure function And its prepare and apply
Technical field
The invention belongs to technical field of soil remediation, and in particular to one kind has improved soil, activating soil, has regulation and control concurrently The bioremediation agents of microbiological paper method function are prepared and applied in continuous cropping soil.
Background technology
Warmhouse booth is applied with the extension of Cultivation time and the long-term accumulated of fertile medicine so that with Nutrient Cycling, organic Matter is decomposed and disease-resistant related microorganism species unbalance of structure, causes the increase of soil-borne pathogen quantity, soil enzymatic activities reduction, soil , there are a series of ecological functions by edaphon such as soil-borne disease is aggravated, fertility is reduced, physicochemical character changes in nutrient imbalance Soil degradation problem, causes the yield of vegetables to decline caused by degenerating, quality variation, and the sustainable of vegetables industry is hindered by serious Development and food security, are badly in need of environment-friendly, economical and effective and suitable for the biological agent of facilities vegetable degenerated soil restoration of the ecosystem, It can ensure that the ecological functions of geobiont are not destroyed on the basis of the supply of effective guarantee product high-quality.
Hebei province's corn, wheat stalk amount are annual up to more than 30,000,000 tons, and the method for conventional incineration had not only polluted environment but also unrestrained Take resource, and by stalk under hypoxia biomass pyrolytic, the gas of generation can be used for resident living, and product is biology Charcoal, is a kind of excellent fertilizer.The nutrient needed containing abundant organic matter, potassium and plant, the larger ratio surface of charcoal Product, flourishing pore structure, imparting it improves soil physics structure, improves soil organic carbon, increases soil fertility Potentiality;The space of storage and existence can be provided for nutrient, microorganism, work(that is nutrient slowly-released and improving viable bacteria shelf life is reached Effect.In today that global resources are increasingly deficient, Soil Environmental Pollution problem is on the rise, biomass carbon is a kind of renewable energy Source, the advantage with green and sustainable development.
Containing abundant nutrient in mushroom bran, organic matter, full nitrogen, full phosphorus, full potassium, the content of rapid available phosphorus can meet plant Healthy growth, while its loose structure can be effectively improved the crumb structure of soil, effectively keep soil from packing together.Chicken manure is one Excellent organic fertilizer is planted, but the application amount at present in canopy room is excessive, there is the potential danger of heavy metal in soil and antibiotic residue Evil, therefore efficiently administration turns into inevitable to its decrement.
For the above state of the art, if can be made full use of above-mentioned resource and solve the problems, such as assessing index, certainly will have There are potential application prospect and wide development space.
The content of the invention
The invention provides a kind of bioremediation agents with improved soil and regulating and controlling microbial population structure function, go forward side by side One step provides its preparation method and application, and the development of the renovation agent can effectively solve a large amount of edible fungus brans and arbitrarily stack, cause The wasting of resources and problem of environmental pollution;A large amount of corns, wheat stalk and corncob can be also solved arbitrarily to accumulate, arbitrarily burn band The problem of environmental pollution come, makes each organic matter resource efficiently be utilized.
The main original for the bioremediation agents with improved soil and regulating and controlling microbial population structure function that the present invention is provided Expect for charcoal, asparagus mushroom bran, chicken manure and complex function micro organism powder, to be formulated by optimized proportion.
Preferably, wherein chicken manure, charcoal and mushroom bran mass ratio are 4:0.5-1:4-6.
It is further preferred that wherein the mass ratio of chicken manure, mushroom bran and charcoal is 4:1:5.
The complex function microorganism includes bacillus amyloliquefaciens WS-8, bacillus subtilis BSD-2, phosphorus decomposing gemma bar Bacterium and colloid bacillus cereus.
The complex function microorganism preparation method is:By the bacillus amyloliquefaciens WS-8(Deposit number is CGMCC No.11787), bacillus subtilis BSD-2(Deposit number is CGMCC No.8440), solution Bacillus phosphorus and colloid Bacillus, fermented and cultured is carried out according to its respective culture medium, and mixing is spray-dried after fermentation, obtains complex function micro- Biological dry powder.
Preferably, added during spray drying in mixed fermentation liquid its quality 6%-10% calcium carbonate, 8-12%-ring paste Essence and 2% magnesium sulfate are as carrier, and the condition of spray drying is:175 DEG C of inlet temperature, 85 DEG C of outlet temperature, pump speed 10mL/ Min, wind speed 30m3/ min, material temperature is 28 DEG C.
Preferably, the bacillus amyloliquefaciens WS-8, bacillus subtilis BSD-2, solution Bacillus phosphorus and colloid bud Spore bacillus is according to spore count 1:1:1:1 ratio mixing.
Preferably, the quality of the complex function microbial dry powder is the chicken manure, mushroom bran and biomass carbon gross mass 20%。
The preparation method of above-mentioned bioremediation agents, it is characterised in that:By the biomass carbon, mushroom bran and chicken manure according to proportioning Mixture, adds complex function microbial dry powder, is granulated after mixing through comminutor, obtains particle diameter 2-4mm renovation agent particle.
The present invention also provides the applications of above-mentioned bioremediation agents, i.e., applied in the form of bottom application, sowing amount for 400 kilograms/ Mu, for improving microbial bacteria group structure, improving soil enzymatic activities, improvement soil physico-chemical property, prevents and treats soil-borne disease.
The present invention has following significant advantage compared with prior art:
The bioremediation agents with improved soil and regulating and controlling microbial population structure function of the present invention, can be effectively improved continuous cropping soil The microbiological paper method of earth, using complex microorganism dry powder as core, is aided with charcoal, three kinds of nutritional auxiliaries of mushroom bran and chicken manure, Charcoal, mushroom bran, chicken manure has been obtained efficient utilization, effectively reduce chicken manure, corn, wheat stalk are caused to environment two Secondary pollution, can effectively safeguard the residence living environment of people, beneficial to the cycle development for promoting agricultural economy.
Biomass carbon has the function of water suction, water conservation and purified water well, and the stink of adsorbable organic fertilizer purifies canopy room In air;Soil carbon sink can be effectively lifted, the soil weight is reduced, and the activity space survived is provided for microorganism, and can be had Excessive nutrient content in effect absorption soil, to reach the effect of sustained release.
The use of bioremediation agents of the present invention, can effectively facilitate the growth of crop root, improve the microorganism species of soil Structure, activation soil vitality makes soil nutrient obtain Efficient Conversion and utilization there is provided the activity of soil enzyme, reduces soil-borne disease Generation, be easy to the production of green vegetables.
Embodiment
Technical scheme is described in detail with specific embodiment below.
Biomass carbon used is corncob charcoal in following examples, but is not limited to this, and its each nutrient content is:Entirely Nitrogen 1.96g/kg, the full g/kg of phosphorus 2.43, the full g/kg of potassium 16.40, organic matter 199.43 g/kg, available nitrogen 37.80mg/kg, speed Imitate phosphorus 241.75mg/kg, available potassium 15.94mg/kg, pH9.42;Charcoal possesses the physical arrangement of hole, can be carried for microorganism For production space, nutrient in also adsorbable soil, beneficial to the sustained release of nutrient.
Mushroom bran used is asparagus mushroom bran in following examples, but is not limited to this, and its each nutrient content is:Full nitrogen 11.48g/kg, the full g/kg of phosphorus 2.43, the full g/kg of potassium 9.60, organic matter 691.78 g/kg, available nitrogen 719.60mg/kg, speed Phosphorus 123.00mg/kg, available potassium 9.06mg/kg, pH6.91 are imitated, additionally containing substantial amounts of crude protein, crude fibre, all kinds of amino Acid, can be beneficial to the growth of plant.
The nutrient content of fermentation maturity chicken manure is:Full nitrogen 23.40 g/kg, the full g/kg of phosphorus 23.20, the full g/kg of potassium 8.30, Organic matter 255.00 g/kg, available nitrogen 1621.10mg/kg, rapid available phosphorus 1125.55mg/kg, available potassium 8.17mg/kg, pH7.84。
The preparation of the complex microorganism dry powder of embodiment 1
By bacillus amyloliquefaciens WS-8, bacillus subtilis BSD-2, Bacillus phosphorus, colloid bacillus cereus are solved, it is each according to its From culture medium carry out fermented and cultured, with spore count 1 after fermentation:1:1:After 1 ratio mixing, using-cyclodextrin, calcium carbonate It is wall material with magnesium sulfate, the addition of-cyclodextrin is 8-12%, and the addition of calcium carbonate is 6-10%, the addition of magnesium sulfate 2%, above addition is the mass percent for accounting for mixed fermentation liquid.
As shown in Table 1, composite microbial bacteria is 10%, the optimum addition of calcium carbonate in the optimum addition of-cyclodextrin For 8%, the addition 2% of magnesium sulfate, using 175 DEG C of inlet temperature, 85 DEG C of outlet temperature, pump speed 10mL/min, wind speed 30m3/ Min, material temperature is 28 DEG C, can be beneficial to form powdery easily collecting, and collection powder rate is high.Spore count living can concentrate 10 times, obtain as 100 Hundred million/gram dry powder.
Influence of the different wall material concentrations of table 1 to finished product
The Different Nutrition additive compound ratio of embodiment 2 is screened
7 are set to handle, processing 1:Chicken manure, mushroom bran, the mass ratio of charcoal are 4:1:4;Processing 2:Chicken manure, mushroom bran, charcoal Mass ratio is 4:1:5;Processing 3:Chicken manure, mushroom bran, the mass ratio of charcoal are 4:1:6;Processing 4:Chicken manure, mushroom bran, charcoal Mass ratio is 4:0.5:4;Processing 5:Chicken manure, mushroom bran, the mass ratio of charcoal are 4:0.5:5;Processing 6:Chicken manure, mushroom bran, biology The mass ratio of charcoal is 4:0.5:6;The soil of each processing addition suitable quality makes the soil quality of each processing equal, after mixing Basin is filled, using pure soil as blank control;
The statistical result of thick different disposal cucumber plant height, stem, root length and the number of blade is determined from table 2, with compareing and its elsewhere Reason is compared, and processing 2 is remarkably improved the plant height of cucumber, stem slightly, increases the quantity of blade, and its resultant effect is optimal.Determine different The result of dry matter weight of cucumber different parts is handled from table 3, processing 2 and processing each position dry matter weight of 3 pairs of cucumber The combined influence effect of amount illustrates that the addition of mushroom bran should not be excessive better than processing 4, processing 5 and processing 6.Processing 1,2 and of processing Handle the addition difference that 3 three processing are organic fertilizer, it is seen that the addition of organic fertilizer is big to each position dry of cucumber On the contrary almost, leaf weight, petiole weight, tendril weight, the stem of 2 cucumber of processing are significantly higher than processing 3 to impact effect again, handle 3 cucumber In addition to spending and being higher than processing 2 again, other each indexs are worse than processing 2.
The comprehensive influence to cucumber morphological index and different parts amount of dry matter, it is determined that 2 chicken manures of processing, mushroom bran, charcoal Mass ratio be 4:1:5 best results.
The morphological index of the different disposal cucumber of table 2
The dry matter weight of the different disposal cucumber of table 3
The preparation of the renovation agent of embodiment 3
Bacillus subtilis BSD-2, bacillus amyloliquefaciens WS-8, solution Bacillus phosphorus(The present embodiment selects huge gemma bar Bacterium P1, but not limited to this bacterial strain)And colloid bacillus cereus(The present embodiment selects colloid bacillus cereus PM13, but not limited to this bacterium Strain)Individually cultivated using suitable method, by spore count 1:1:1:1 ratio compounding, using spray-drying apparatus, with 10% - cyclodextrin, 8% calcium carbonate and 2% magnesium sulfate are wall material;Select 175 DEG C of inlet temperature, 85 DEG C of outlet temperature, pump speed 10mL/min, wind speed 30m3/ min, material temperature is 28 DEG C of spray drying condition, dust can obtain 10,000,000,000/gram dry powder.
Chicken manure:Mushroom bran:The optimum quality ratio of three kinds of nutritional auxiliaries of biomass carbon is 4:1:5, complex microorganism dry powder plus It is the 20% of nutritional auxiliary gross mass to enter amount, granulates the particle for obtaining particle diameter 2-4mm after mixing through comminutor.
The application effect of the renovation agent of embodiment 4
Application test sets three processing in progress in continuous cropping canopy room for many years, and processing 1 is commercially available soil conditioner, handles 2 to repair Multiple agent, processing 3 is blank control, and modifying agent and renovation agent sowing amount are 400 kgs/acre, and cucumber root is taken in the cucumber phase of uprooting plants after their edible portions have been harvested The soil test index of correlation on border.
4.1 different disposal cucumber rhizosphere Soil Microorganism quantity and population structure
The measurement result of Bacterial diversity quantity compared with the control, is applied from table 4 in different disposal cucumber rhizosphere soil Modifying agent and renovation agent processing can substantially reduce the quantity of fungi and sharp spore bacterium in phase rhizosphere soil of uprooting plants after their edible portions have been harvested, wherein at renovation agent Preferably, compared with the control, the reduced rate of fungi and sharp spore bacterium number amount is respectively 42.39% and 42.85% to the effect of reason.With it is right Photograph ratio, can significantly improve the quantity of bacterium and actinomyces in rhizosphere soil, wherein repairing using modifying agent and renovation agent processing The inoculation gaining rate of the best results of multiple agent processing, actinomyces and bacterium is 9.39% and 54.97%.The ratio of bacterium and fungi and put The ratio of line bacterium and fungi is compared with the control, hence it is evident that increase, wherein nearly 2 times of gaining rate, the administration of renovation agent more conducively rhizosphere soil Middle microbial bacteria group structure changes to bacteria type, beneficial to the incidence of reduction fungal disease.Comprehensive repair agent processing pair can be cultivated Fungi, bacterium, the influence of actinomyces and Fusarium oxysporum quantity, applying for renovation agent can be beneficial to improve in soil cultivate micro- The reduction of soil-borne pathogen quantity in the quantity and Bacterial community of biological flora, reduction continuous cropping soil, beneficial to the hair of reduction disease Raw rate.
Micro organism quantity and population structure in the rhizosphere soil of table 4
4.2 different disposals prevent and treat the effect of soil-borne disease
In the whole breeding time of cucumber, the disease index of cucumber fusarium axysporum is counted, and calculates comprehensive protection effect, as a result by table 5 understand, compared with the control, and the application of modifying agent and renovation agent can significantly reduce the disease index of soil-borne disease, improve to soil biography The best results of the protection effect of disease, wherein renovation agent processing, protection effect is up to 59.06%.
The disease index and protection effect of the cucumber fusarium axysporum of table 5
The soil enzymatic activities of 4.3 different disposal cucumber rhizospheres
Different disposal uproot plants after their edible portions have been harvested phase cucumber rhizosphere soil enzymatic activities measurement result from table 6, the application profit of modifying agent and renovation agent In the raising of soil urease, invertase, catalase and Soil neutral phosphatase activity, wherein renovation agent is applied to each related enzyme activity The increased effect of property is best;Compared with the control, the active increasing of urase, invertase, catalase and Soil neutral phosphatase in soil Height ratio is respectively 9.24%, 7.86%, 36.67% and 29.06%.
The rhizosphere soil enzyme activity of table 6
4.4 cucumber are uprooted plants after their edible portions have been harvested the physicochemical property of phase cucumber rhizosphere soil
The salinity of different disposal cucumber rhizosphere soil, unit weight, the measurement result of water-holding capacity and acid-base value are from table 7, modifying agent With the raising of the reduction applied beneficial to the cucumber rhizosphere soil weight, salt content of renovation agent, and water-holding capacity, acid-base value, it is beneficial to The salinization of soil of soil is prevented, improves the structure of soil;Simultaneously beneficial to the water content of increase soil;The wherein influence of soil-repairing agent Best results.Compared with the control, water-holding capacity improves 15.03%, unit weight reduction by 15.20%.
The physicochemical property of the soil of table 7

Claims (10)

1. a kind of bioremediation agents with improved soil and regulating and controlling microbial population structure function, it is characterised in that:It includes Complex function microorganism, biomass carbon, mushroom bran and chicken manure.
2. bioremediation agents according to claim 1, it is characterised in that:Wherein chicken manure, mushroom bran and biomass carbon add matter Amount is than being 4:0.5-1:4-6.
3. bioremediation agents according to claim 2, it is characterised in that:Wherein chicken manure, mushroom bran and biomass carbon add matter Amount is than being 4:1:5.
4. bioremediation agents according to claim 1, it is characterised in that:The complex function microorganism includes solution starch bud Spore bacillus WS-8, bacillus subtilis BSD-2, solution Bacillus phosphorus and colloid bacillus cereus.
5. bioremediation agents according to claim 4, it is characterised in that:The complex function microorganism preparation method For:By the bacillus amyloliquefaciens WS-8, bacillus subtilis BSD-2, solution Bacillus phosphorus and colloid bacillus cereus, foundation Its respective culture medium carries out fermented and cultured, and mixing is spray-dried after fermentation, obtains complex function microbial dry powder.
6. bioremediation agents according to claim 5, it is characterised in that:During spray drying it is added in mixed fermentation liquid Quality 6%-10% calcium carbonate, 8-12%-cyclodextrin and 2% magnesium sulfate as carrier, the condition of spray drying is:Import 175 DEG C of temperature, 85 DEG C of outlet temperature, pump speed 10mL/min, wind speed 30m3/ min, material temperature is 28 DEG C.
7. bioremediation agents according to claim 5, it is characterised in that:The bacillus amyloliquefaciens WS-8, withered grass bud Spore bacillus BSD-2, solution Bacillus phosphorus and colloid bacillus cereus are according to spore count 1:1:1:1 ratio mixing.
8. bioremediation agents according to claim 5, it is characterised in that:The quality of the complex function microbial dry powder is The 20% of the chicken manure, mushroom bran and biomass carbon gross mass.
9. the preparation method of any one of the claim 1-8 bioremediation agents, it is characterised in that:By the chicken manure, mushroom bran and Biomass carbon adds complex function microbial dry powder, granulated after mixing through comminutor according to proportioning mixture, obtains particle diameter 2-4mm Renovation agent particle.
10. the application of any one of the claim 1-8 bioremediation agents, it is characterised in that:Applied, applied in the form of bottom application Measure as 400 kgs/acre, for improving microbial bacteria group structure, improving soil enzymatic activities, improvement soil physico-chemical property, preventing and treating soil is passed Disease.
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