CN107075507B - 用于在细胞表面上表达多肽的组合物和方法 - Google Patents

用于在细胞表面上表达多肽的组合物和方法 Download PDF

Info

Publication number
CN107075507B
CN107075507B CN201580059777.8A CN201580059777A CN107075507B CN 107075507 B CN107075507 B CN 107075507B CN 201580059777 A CN201580059777 A CN 201580059777A CN 107075507 B CN107075507 B CN 107075507B
Authority
CN
China
Prior art keywords
ser
thr
polypeptide
amino acid
poi
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201580059777.8A
Other languages
English (en)
Chinese (zh)
Other versions
CN107075507A (zh
Inventor
阿龙·迈克尔·林
安德鲁·克鲁瑟
阿施施·曼格里克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leland Stanford Junior University
Original Assignee
Leland Stanford Junior University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leland Stanford Junior University filed Critical Leland Stanford Junior University
Publication of CN107075507A publication Critical patent/CN107075507A/zh
Application granted granted Critical
Publication of CN107075507B publication Critical patent/CN107075507B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70532—B7 molecules, e.g. CD80, CD86
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7155—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2818—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD28 or CD152
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
    • G01N2333/70521—CD28, CD152
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
    • G01N2333/70532—B7 molecules, e.g. CD80, CD86
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/715—Assays involving receptors, cell surface antigens or cell surface determinants for cytokines; for lymphokines; for interferons
    • G01N2333/7155—Assays involving receptors, cell surface antigens or cell surface determinants for cytokines; for lymphokines; for interferons for interleukins [IL]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201580059777.8A 2014-11-10 2015-11-09 用于在细胞表面上表达多肽的组合物和方法 Active CN107075507B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462077756P 2014-11-10 2014-11-10
US62/077,756 2014-11-10
PCT/US2015/059787 WO2016077249A1 (en) 2014-11-10 2015-11-09 Compositions and methods for expressing polypeptides on the surface of cells

Publications (2)

Publication Number Publication Date
CN107075507A CN107075507A (zh) 2017-08-18
CN107075507B true CN107075507B (zh) 2021-08-24

Family

ID=55954905

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201580059777.8A Active CN107075507B (zh) 2014-11-10 2015-11-09 用于在细胞表面上表达多肽的组合物和方法

Country Status (5)

Country Link
US (1) US10829771B2 (https=)
EP (1) EP3218477A4 (https=)
JP (2) JP7235436B2 (https=)
CN (1) CN107075507B (https=)
WO (1) WO2016077249A1 (https=)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11473081B2 (en) * 2016-12-12 2022-10-18 xCella Biosciences, Inc. Methods and systems for screening using microcapillary arrays
JP7208902B2 (ja) 2016-12-30 2023-01-19 エクセラ・バイオサイエンシーズ・インコーポレイテッド マルチステージサンプル回収システム
US11668021B2 (en) 2017-05-09 2023-06-06 Yale University Basehit, a high-throughput assay to identify proteins involved in host-microbe interaction
JP7390027B2 (ja) * 2017-10-31 2023-12-01 エンコディア， インコーポレイテッド 核酸エンコーディングおよび／または標識を使用する解析のためのキット
CN108085332B (zh) * 2017-12-27 2020-11-27 中国科学院青岛生物能源与过程研究所 一种细胞表面展示有豆壳过氧化物酶的重组酵母菌及其构建方法与应用
WO2020061389A1 (en) * 2018-09-20 2020-03-26 Washington University Engineered microorganisms and methods of making and using same
US12331320B2 (en) 2018-10-10 2025-06-17 The Research Foundation For The State University Of New York Genome edited cancer cell vaccines
EP3890876B1 (en) 2018-12-06 2024-05-01 Xcella Biosciences, Inc. Lateral loading of microcapillary arrays
CN110551644A (zh) * 2019-07-19 2019-12-10 天津科技大学 通过调控细胞壁组成蛋白构建的低产高级醇酿酒酵母菌株
EP4106790A4 (en) * 2020-02-21 2024-02-21 3T Biosciences, Inc. Yeast display libraries, associated compositions, and associated methods of use
KR20230114748A (ko) * 2020-10-28 2023-08-01 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 혈관 염증 감소를 위한 cd47 차단 및 이의 조합 요법
WO2021115497A2 (zh) 2020-12-08 2021-06-17 和铂医药(上海)有限责任公司 蛋白-药物偶联物和定点偶联方法
KR20240021211A (ko) * 2021-06-10 2024-02-16 얀센 바이오테크 인코포레이티드 Klk2-gpi 융합 단백질에 대한 핵산 코딩, 재조합 세포 및 이의 용도
CN118546912A (zh) * 2024-05-29 2024-08-27 浙江毓昌生物技术有限公司 一种高稳定性双碱基酶Kex2突变体的表达纯化方法
CN118726441B (zh) * 2024-06-20 2025-06-03 广州大学 一种灵芝表面展示系统及其制备方法和应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101622274A (zh) * 2006-12-12 2010-01-06 比奥雷克西斯药物公司 转铁蛋白融合蛋白库
US20120077252A1 (en) * 2011-05-03 2012-03-29 Verdezyne, Inc. Biological methods for preparing adipic acid
US20120208769A1 (en) * 2005-12-02 2012-08-16 Peter Jon Nelson Chemokine-Mucin Fusions Linked to Glycosylphosphatidylinositol (GPI)-Anchors in Tissue Regeneration and as Tumour Immune Adjuvants

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003235579A (ja) * 2002-02-19 2003-08-26 Bio Energy Kk 凝集タンパク質Ｆｌｏ１を用いる細胞表層発現系の最適化
JP2010517577A (ja) 2007-02-09 2010-05-27 メディミューン，エルエルシー 酵母細胞原形質膜による抗体ライブラリー提示
US9127087B2 (en) 2008-12-01 2015-09-08 University Of Maryland, Baltimore High affinity recombinant sea lamprey antibodies selected by a Yeast Surface Display platform

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120208769A1 (en) * 2005-12-02 2012-08-16 Peter Jon Nelson Chemokine-Mucin Fusions Linked to Glycosylphosphatidylinositol (GPI)-Anchors in Tissue Regeneration and as Tumour Immune Adjuvants
CN101622274A (zh) * 2006-12-12 2010-01-06 比奥雷克西斯药物公司 转铁蛋白融合蛋白库
US20120077252A1 (en) * 2011-05-03 2012-03-29 Verdezyne, Inc. Biological methods for preparing adipic acid

Also Published As

Publication number Publication date
JP7235436B2 (ja) 2023-03-08
CN107075507A (zh) 2017-08-18
WO2016077249A1 (en) 2016-05-19
JP2018500009A (ja) 2018-01-11
JP2021101712A (ja) 2021-07-15
US10829771B2 (en) 2020-11-10
EP3218477A1 (en) 2017-09-20
US20180037898A1 (en) 2018-02-08
EP3218477A4 (en) 2018-07-18

Similar Documents

Publication Publication Date Title
CN107075507B (zh) 用于在细胞表面上表达多肽的组合物和方法
CN101802006B (zh) 展示结合剂
Steffan et al. The role of TBP in rDNA transcription by RNA polymerase I in Saccharomyces cerevisiae: TBP is required for upstream activation factor-dependent recruitment of core factor.
KR102191739B1 (ko) 변형된 구제역 바이러스 3c 프로테아제, 조성물 및 이의 방법
US11884974B2 (en) TIM-3 nanobody, a preparation method thereof, and use thereof
US11236132B2 (en) Nepovirus coat protein fusion polypeptides and their use
CN101896603A (zh) 使用遗传修饰的生物产生生物质降解酶
CN116589569B (zh) 一种抗h7n9亚型流感病毒ha蛋白的纳米抗体及其噬菌体展示文库的构建方法和应用
KR20220013569A (ko) Kras 항원을 표적화하는 면역요법용 구조체
KR102277529B1 (ko) 변형된 피코르나바이러스 3c 프로테아제 및 그의 방법
CN116375851B (zh) 一种抗禽流感病毒np蛋白的纳米抗体及其制备方法与应用
CN117736316A (zh) 抗新布尼亚病毒np抗原的单克隆抗体及其应用和产品
JP2013527752A (ja) 融合ポリペプチドｈｐｖ抗原を用いたｈｐｖ抗体スクリーニング方法
JP4440464B2 (ja) アッセイ方法
HK1243124A1 (en) Compositions and methods for expressing polypeptides on the surface of cells
HK1243124B (zh) 用於在细胞表面上表达多肽的组合物和方法
CN115128266A (zh) 用于检测自身抗体的方法和试剂
CN110872354B (zh) 哺乳动物细胞重组抗人tk1的鸡源的单克隆抗体、单链抗体及其制备方法和应用
JP2002515254A (ja) 酵素活性組換えヒトβ−トリプターゼ及びその生産方法
US20220281957A1 (en) Modified human variable domains
CN108410901B (zh) 无抗性筛选双抗原锚定表达载体pLQ2a及制备方法
CN117229385A (zh) Pd-1变体及其用途
CN115594757B (zh) 抗新型冠状病毒的中和抗体及其制备方法、制剂和用途
CN115594758B (en) Nanometer antibody for resisting novel coronavirus SARS-CoV-2 and its preparation method and use
CN119306824B (zh) 一种全价展示scFv的通用型M13噬菌体探针及其构建方法和应用

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1243124

Country of ref document: HK

GR01 Patent grant
GR01 Patent grant
TG01 Patent term adjustment

Free format text: NEW EXPIRY DATE: 20360301

TG01 Patent term adjustment