CN107022029A - 高特异高灵敏检测甲胎蛋白的单克隆抗体及试剂盒和应用 - Google Patents
高特异高灵敏检测甲胎蛋白的单克隆抗体及试剂盒和应用 Download PDFInfo
- Publication number
- CN107022029A CN107022029A CN201710179442.0A CN201710179442A CN107022029A CN 107022029 A CN107022029 A CN 107022029A CN 201710179442 A CN201710179442 A CN 201710179442A CN 107022029 A CN107022029 A CN 107022029A
- Authority
- CN
- China
- Prior art keywords
- monoclonal antibody
- antibody
- chain variable
- detection
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57438—Specifically defined cancers of liver, pancreas or kidney
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/33—Crossreactivity, e.g. for species or epitope, or lack of said crossreactivity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
- C07K2317/565—Complementarity determining region [CDR]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
- C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/471—Pregnancy proteins, e.g. placenta proteins, alpha-feto-protein, pregnancy specific beta glycoprotein
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gastroenterology & Hepatology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710179442.0A CN107022029B (zh) | 2017-03-23 | 2017-03-23 | 高特异高灵敏检测甲胎蛋白的单克隆抗体及试剂盒和应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710179442.0A CN107022029B (zh) | 2017-03-23 | 2017-03-23 | 高特异高灵敏检测甲胎蛋白的单克隆抗体及试剂盒和应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107022029A true CN107022029A (zh) | 2017-08-08 |
CN107022029B CN107022029B (zh) | 2021-03-23 |
Family
ID=59525822
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710179442.0A Active CN107022029B (zh) | 2017-03-23 | 2017-03-23 | 高特异高灵敏检测甲胎蛋白的单克隆抗体及试剂盒和应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107022029B (zh) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107022030A (zh) * | 2017-03-23 | 2017-08-08 | 广东谷盈生物科技产业研究院有限公司 | 检测甲胎蛋白的单克隆抗体及试剂盒和应用 |
CN108387735A (zh) * | 2017-11-27 | 2018-08-10 | 南京天纵易康生物科技股份有限公司 | 一种基于双分子荧光互补技术的afp检测试剂盒、制备及使用方法 |
CN108484762A (zh) * | 2018-03-20 | 2018-09-04 | 南京京达生物技术有限公司 | 一种甲胎蛋白检测用抗体IgM及应用 |
CN111487417A (zh) * | 2020-03-16 | 2020-08-04 | 北京维德维康生物技术有限公司 | Mcr-1耐药蛋白双抗夹心elisa检测试剂盒及检测方法 |
CN113173994A (zh) * | 2021-05-19 | 2021-07-27 | 深圳市国创纳米抗体技术有限公司 | 一种抗afp的纳米抗体1a7及其应用 |
CN113234166A (zh) * | 2021-05-19 | 2021-08-10 | 深圳市国创纳米抗体技术有限公司 | 一种抗afp的纳米抗体1c5及其应用 |
CN113583133A (zh) * | 2021-09-06 | 2021-11-02 | 深圳市国创纳米抗体技术有限公司 | 一种抗afp的纳米抗体1b9及其应用 |
CN114605531A (zh) * | 2022-04-08 | 2022-06-10 | 北京科跃中楷生物技术有限公司 | 荧光微球标记的抗体及其应用 |
CN115894683A (zh) * | 2022-12-22 | 2023-04-04 | 山东纳睿博恩生物医药科技有限公司 | 检测甲胎蛋白的单克隆抗体及其应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20130088576A (ko) * | 2012-01-31 | 2013-08-08 | (주) 프로탄바이오 | Afp에 특이적으로 결합하는 항체, 이를 생산하는 하이브리도마 세포주, 및 이를 이용한 간암 진단용 키트 |
CN105037544A (zh) * | 2015-07-21 | 2015-11-11 | 南昌大佳科技有限公司 | 基于afp抗原的afp纳米抗体a83 |
CN107022030A (zh) * | 2017-03-23 | 2017-08-08 | 广东谷盈生物科技产业研究院有限公司 | 检测甲胎蛋白的单克隆抗体及试剂盒和应用 |
-
2017
- 2017-03-23 CN CN201710179442.0A patent/CN107022029B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20130088576A (ko) * | 2012-01-31 | 2013-08-08 | (주) 프로탄바이오 | Afp에 특이적으로 결합하는 항체, 이를 생산하는 하이브리도마 세포주, 및 이를 이용한 간암 진단용 키트 |
CN105037544A (zh) * | 2015-07-21 | 2015-11-11 | 南昌大佳科技有限公司 | 基于afp抗原的afp纳米抗体a83 |
CN107022030A (zh) * | 2017-03-23 | 2017-08-08 | 广东谷盈生物科技产业研究院有限公司 | 检测甲胎蛋白的单克隆抗体及试剂盒和应用 |
Non-Patent Citations (2)
Title |
---|
孙一帆等: "抗人甲胎蛋白单克隆抗体的制备", 《中国医药生物技术》 * |
杨书豪等: "抗人甲胎蛋白单克隆抗体的制备及鉴定", 《细胞与分子免疫学杂志》 * |
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107022030A (zh) * | 2017-03-23 | 2017-08-08 | 广东谷盈生物科技产业研究院有限公司 | 检测甲胎蛋白的单克隆抗体及试剂盒和应用 |
CN108387735A (zh) * | 2017-11-27 | 2018-08-10 | 南京天纵易康生物科技股份有限公司 | 一种基于双分子荧光互补技术的afp检测试剂盒、制备及使用方法 |
CN108484762A (zh) * | 2018-03-20 | 2018-09-04 | 南京京达生物技术有限公司 | 一种甲胎蛋白检测用抗体IgM及应用 |
CN111487417B (zh) * | 2020-03-16 | 2024-01-23 | 北京维德维康生物技术有限公司 | Mcr-1耐药蛋白双抗夹心elisa检测试剂盒及检测方法 |
CN111487417A (zh) * | 2020-03-16 | 2020-08-04 | 北京维德维康生物技术有限公司 | Mcr-1耐药蛋白双抗夹心elisa检测试剂盒及检测方法 |
CN113173994A (zh) * | 2021-05-19 | 2021-07-27 | 深圳市国创纳米抗体技术有限公司 | 一种抗afp的纳米抗体1a7及其应用 |
CN113234166A (zh) * | 2021-05-19 | 2021-08-10 | 深圳市国创纳米抗体技术有限公司 | 一种抗afp的纳米抗体1c5及其应用 |
CN113173994B (zh) * | 2021-05-19 | 2022-04-01 | 深圳市国创纳米抗体技术有限公司 | 一种抗afp的纳米抗体1a7及其应用 |
CN113234166B (zh) * | 2021-05-19 | 2022-04-01 | 深圳市国创纳米抗体技术有限公司 | 一种抗afp的纳米抗体1c5及其应用 |
CN113583133A (zh) * | 2021-09-06 | 2021-11-02 | 深圳市国创纳米抗体技术有限公司 | 一种抗afp的纳米抗体1b9及其应用 |
CN113583133B (zh) * | 2021-09-06 | 2022-04-22 | 深圳市国创纳米抗体技术有限公司 | 一种抗afp的纳米抗体1b9及其应用 |
CN114605531A (zh) * | 2022-04-08 | 2022-06-10 | 北京科跃中楷生物技术有限公司 | 荧光微球标记的抗体及其应用 |
CN114605531B (zh) * | 2022-04-08 | 2022-08-09 | 北京科跃中楷生物技术有限公司 | 荧光微球标记的抗体及其应用 |
CN115894683A (zh) * | 2022-12-22 | 2023-04-04 | 山东纳睿博恩生物医药科技有限公司 | 检测甲胎蛋白的单克隆抗体及其应用 |
CN115894683B (zh) * | 2022-12-22 | 2023-08-04 | 山东纳睿博恩生物医药科技有限公司 | 检测甲胎蛋白的单克隆抗体及其应用 |
Also Published As
Publication number | Publication date |
---|---|
CN107022029B (zh) | 2021-03-23 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107022029A (zh) | 高特异高灵敏检测甲胎蛋白的单克隆抗体及试剂盒和应用 | |
CN107022030A (zh) | 检测甲胎蛋白的单克隆抗体及试剂盒和应用 | |
JP7209464B2 (ja) | ヒトインターロイキン-2に対する免疫刺激性モノクローナル抗体 | |
CN109206514B (zh) | Tslp单克隆抗体及其制备方法和应用 | |
CN104862283B (zh) | 一对高特异性高亲和力结合人肌红蛋白的单克隆抗体及其应用 | |
US20090280503A1 (en) | Method for detecting and treating skin disorders | |
CN106916225A (zh) | 一种检测n端脑钠肽前体的单克隆抗体及其杂交瘤细胞株和应用 | |
CN109734805A (zh) | 抗ck20蛋白单克隆抗体、细胞系及其制备方法和应用 | |
CN105273083A (zh) | 抗人芳香烃受体单克隆抗体及其用途 | |
CN108700588A (zh) | 用于检测和治疗胃癌的组合物和方法 | |
CN109596839A (zh) | 人和肽素快速定量检测方法与试剂盒 | |
CN112094348A (zh) | 抗人Tim3抗体或其功能性片段及其应用 | |
EP0142345A2 (en) | Anti-idiotypic vaccine employing anti-idiotypic monoclonal antibodies | |
KR20180105647A (ko) | 난소암의 검출 및 치료를 위한 조성물 및 방법 | |
US11906520B2 (en) | Composition and methods for detecting cancer | |
CN108588033A (zh) | 杂交瘤细胞株、cd31单克隆抗体、制备方法及应用 | |
CN108779497A (zh) | 足糖萼蛋白和tra-相关抗体、制备方法和作为抗癌治疗剂的用途 | |
JPS61180799A (ja) | モノクローナル抗体 | |
CN109535255A (zh) | 一种抗人cd26抗体及其在检测试剂盒中的应用 | |
CN110105449A (zh) | 一种特异性结合vegf的抗体及用途 | |
CN108752422A (zh) | 一种微小隐孢子虫检测用tsp4多肽序列及用途 | |
CN106029094A (zh) | 新的抗adam17抗体及其用于治疗癌症的用途 | |
CN104087557B (zh) | 杂交瘤细胞株及基于杂交瘤细胞株的抗Nodal抗体和检测肿瘤细胞方面的应用 | |
CN108623684B (zh) | 一种识别贝伐珠单抗的单克隆抗体及其应用 | |
CN110133278A (zh) | 一种用于检测人vegf蛋白表达水平的体外试剂盒 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
DD01 | Delivery of document by public notice |
Addressee: Li Jiawei Document name: Deemed not to have been notified |
|
DD01 | Delivery of document by public notice | ||
TR01 | Transfer of patent right |
Effective date of registration: 20220321 Address after: 510630 Room 302, No. 894, Tianhe North Road, Tianhe District, Guangzhou, Guangdong Province Patentee after: Guangzhou Purui Biotechnology Co.,Ltd. Address before: 510632 No. 601, Whampoa Avenue, Guangzhou, Guangdong Patentee before: Jinan University |
|
TR01 | Transfer of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Monoclonal antibody and kit for high specificity and sensitivity detection of alpha fetoprotein and its application Effective date of registration: 20220719 Granted publication date: 20210323 Pledgee: Bank of China Limited by Share Ltd. Guangzhou Liwan branch Pledgor: Guangzhou Purui Biotechnology Co.,Ltd. Registration number: Y2022980010713 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |