CN107022029B - 高特异高灵敏检测甲胎蛋白的单克隆抗体及试剂盒和应用 - Google Patents
高特异高灵敏检测甲胎蛋白的单克隆抗体及试剂盒和应用 Download PDFInfo
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- CN107022029B CN107022029B CN201710179442.0A CN201710179442A CN107022029B CN 107022029 B CN107022029 B CN 107022029B CN 201710179442 A CN201710179442 A CN 201710179442A CN 107022029 B CN107022029 B CN 107022029B
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57438—Specifically defined cancers of liver, pancreas or kidney
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/33—Crossreactivity, e.g. for species or epitope, or lack of said crossreactivity
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
- C07K2317/565—Complementarity determining region [CDR]
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
- C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/471—Pregnancy proteins, e.g. placenta proteins, alpha-feto-protein, pregnancy specific beta glycoprotein
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CN201710179442.0A CN107022029B (zh) | 2017-03-23 | 2017-03-23 | 高特异高灵敏检测甲胎蛋白的单克隆抗体及试剂盒和应用 |
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CN107022029A CN107022029A (zh) | 2017-08-08 |
CN107022029B true CN107022029B (zh) | 2021-03-23 |
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Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107022030B (zh) * | 2017-03-23 | 2020-08-28 | 广东谷盈生物科技产业研究院有限公司 | 检测甲胎蛋白的单克隆抗体及试剂盒和应用 |
CN108387735A (zh) * | 2017-11-27 | 2018-08-10 | 南京天纵易康生物科技股份有限公司 | 一种基于双分子荧光互补技术的afp检测试剂盒、制备及使用方法 |
CN108484762B (zh) * | 2018-03-20 | 2021-09-10 | 南京京达生物技术有限公司 | 一种甲胎蛋白检测用抗体IgM及应用 |
CN111487417B (zh) * | 2020-03-16 | 2024-01-23 | 北京维德维康生物技术有限公司 | Mcr-1耐药蛋白双抗夹心elisa检测试剂盒及检测方法 |
CN113173994B (zh) * | 2021-05-19 | 2022-04-01 | 深圳市国创纳米抗体技术有限公司 | 一种抗afp的纳米抗体1a7及其应用 |
CN113234166B (zh) * | 2021-05-19 | 2022-04-01 | 深圳市国创纳米抗体技术有限公司 | 一种抗afp的纳米抗体1c5及其应用 |
CN113583133B (zh) * | 2021-09-06 | 2022-04-22 | 深圳市国创纳米抗体技术有限公司 | 一种抗afp的纳米抗体1b9及其应用 |
CN114605531B (zh) * | 2022-04-08 | 2022-08-09 | 北京科跃中楷生物技术有限公司 | 荧光微球标记的抗体及其应用 |
CN115894683B (zh) * | 2022-12-22 | 2023-08-04 | 山东纳睿博恩生物医药科技有限公司 | 检测甲胎蛋白的单克隆抗体及其应用 |
Citations (3)
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---|---|---|---|---|
KR20130088576A (ko) * | 2012-01-31 | 2013-08-08 | (주) 프로탄바이오 | Afp에 특이적으로 결합하는 항체, 이를 생산하는 하이브리도마 세포주, 및 이를 이용한 간암 진단용 키트 |
CN105037544A (zh) * | 2015-07-21 | 2015-11-11 | 南昌大佳科技有限公司 | 基于afp抗原的afp纳米抗体a83 |
CN107022030A (zh) * | 2017-03-23 | 2017-08-08 | 广东谷盈生物科技产业研究院有限公司 | 检测甲胎蛋白的单克隆抗体及试剂盒和应用 |
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2017
- 2017-03-23 CN CN201710179442.0A patent/CN107022029B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20130088576A (ko) * | 2012-01-31 | 2013-08-08 | (주) 프로탄바이오 | Afp에 특이적으로 결합하는 항체, 이를 생산하는 하이브리도마 세포주, 및 이를 이용한 간암 진단용 키트 |
CN105037544A (zh) * | 2015-07-21 | 2015-11-11 | 南昌大佳科技有限公司 | 基于afp抗原的afp纳米抗体a83 |
CN107022030A (zh) * | 2017-03-23 | 2017-08-08 | 广东谷盈生物科技产业研究院有限公司 | 检测甲胎蛋白的单克隆抗体及试剂盒和应用 |
Non-Patent Citations (2)
Title |
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抗人甲胎蛋白单克隆抗体的制备;孙一帆等;《中国医药生物技术》;20140831;第268页左栏摘要,第268页右栏第4段,第270页右栏第1段,271页左栏第2段 * |
抗人甲胎蛋白单克隆抗体的制备及鉴定;杨书豪等;《细胞与分子免疫学杂志》;19971231;第45-47页 * |
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Denomination of invention: Monoclonal antibody and kit for high specificity and sensitivity detection of alpha fetoprotein and its application Effective date of registration: 20220719 Granted publication date: 20210323 Pledgee: Bank of China Limited by Share Ltd. Guangzhou Liwan branch Pledgor: Guangzhou Purui Biotechnology Co.,Ltd. Registration number: Y2022980010713 |