CN107012123A - 有效建立诱导的多能干细胞的方法 - Google Patents
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- CN107012123A CN107012123A CN201611216942.9A CN201611216942A CN107012123A CN 107012123 A CN107012123 A CN 107012123A CN 201611216942 A CN201611216942 A CN 201611216942A CN 107012123 A CN107012123 A CN 107012123A
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- C—CHEMISTRY; METALLURGY
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
- C12N15/867—Retroviral vectors
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/02—Atmosphere, e.g. low oxygen conditions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/602—Sox-2
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/603—Oct-3/4
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/604—Klf-4
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/606—Transcription factors c-Myc
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2510/00—Genetically modified cells
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- Engineering & Computer Science (AREA)
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- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Transplantation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8484208P | 2008-07-30 | 2008-07-30 | |
| US61/084842 | 2008-07-30 | ||
| US14117708P | 2008-12-29 | 2008-12-29 | |
| US61/141177 | 2008-12-29 | ||
| US20393108P | 2008-12-30 | 2008-12-30 | |
| US61/203931 | 2008-12-30 | ||
| CN200980100065A CN101848994A (zh) | 2008-07-30 | 2009-07-30 | 有效建立诱导的多能干细胞的方法 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200980100065A Division CN101848994A (zh) | 2008-07-30 | 2009-07-30 | 有效建立诱导的多能干细胞的方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107012123A true CN107012123A (zh) | 2017-08-04 |
Family
ID=41610532
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611216942.9A Pending CN107012123A (zh) | 2008-07-30 | 2009-07-30 | 有效建立诱导的多能干细胞的方法 |
| CN200980100065A Pending CN101848994A (zh) | 2008-07-30 | 2009-07-30 | 有效建立诱导的多能干细胞的方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200980100065A Pending CN101848994A (zh) | 2008-07-30 | 2009-07-30 | 有效建立诱导的多能干细胞的方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US9528092B2 (https=) |
| EP (1) | EP2307539B1 (https=) |
| JP (1) | JP5562231B2 (https=) |
| KR (1) | KR101685209B1 (https=) |
| CN (2) | CN107012123A (https=) |
| CA (1) | CA2697621C (https=) |
| WO (1) | WO2010013845A1 (https=) |
Families Citing this family (51)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2010042490A1 (en) * | 2008-10-06 | 2010-04-15 | Boston Medical Center Corporation | A single lentiviral vector system for induced pluripotent (ips) stem cells derivation |
| US10328103B2 (en) | 2009-01-03 | 2019-06-25 | Ray C. Wasielewski | Medical treatment composition comprising mammalian dental pulp stem cells |
| US8470308B2 (en) * | 2009-01-03 | 2013-06-25 | Ray C. Wasielewski | Enhanced medical implant comprising disrupted tooth pulp and tooth particles |
| JP5637354B2 (ja) * | 2009-03-30 | 2014-12-10 | 独立行政法人産業技術総合研究所 | 精製転写因子の調製法と細胞導入技術 |
| CA2770412C (en) | 2009-08-07 | 2018-09-11 | Kyoto University | Method of efficiently establishing induced pluripotent stem cells |
| WO2011031568A2 (en) | 2009-08-27 | 2011-03-17 | Synaptic Research, Llc | A NOVEL PROTEIN DELIVERY SYSTEM TO GENERATE INDUCED PLURIPOTENT STEM (iPS) CELLS OR TISSUE-SPECIFIC CELLS |
| US20120301438A1 (en) * | 2009-09-14 | 2012-11-29 | The Johns Hopkins University | Reprogramming Blood Cells to Pluripotent and Multipotent Stem Cells |
| WO2011062963A2 (en) * | 2009-11-17 | 2011-05-26 | Vitro Diagnositics, Inc. | Induced puripotent stem cells and related methods |
| WO2011159684A2 (en) | 2010-06-15 | 2011-12-22 | Cellular Dynamics International, Inc. | Generation of induced pluripotent stem cells from small volumes of peripheral blood |
| EP2582792B1 (en) * | 2010-06-18 | 2018-10-31 | FUJIFILM Cellular Dynamics, Inc. | Cardiomyocyte medium with dialyzed serum |
| US9506039B2 (en) | 2010-12-03 | 2016-11-29 | Kyoto University | Efficient method for establishing induced pluripotent stem cells |
| EP2655601A4 (en) | 2010-12-22 | 2014-09-10 | Fate Therapeutics Inc | CELL CULTURAL PLATFORM FOR INDIVIDUAL CELL SIZING AND IMPROVED RESOLUTION OF IPSCS |
| CN107988381B (zh) | 2011-07-25 | 2021-05-28 | 国立大学法人京都大学 | 筛选诱导的多能干细胞的方法 |
| KR102031386B1 (ko) * | 2011-10-03 | 2019-10-11 | 닛산 가가쿠 가부시키가이샤 | 다능성 간세포로부터의 거핵구 및/또는 혈소판의 제조 방법 |
| WO2013058403A1 (ja) | 2011-10-21 | 2013-04-25 | 国立大学法人京都大学 | 層流による多能性維持単一分散細胞培養法 |
| JP5999658B2 (ja) | 2011-11-25 | 2016-09-28 | 国立大学法人京都大学 | 多能性幹細胞の培養方法 |
| CN104321426A (zh) * | 2012-03-28 | 2015-01-28 | 石匠株式会社 | 永生化干细胞以及以其生成物为有效成分的药物组合物和药物制剂 |
| AU2013264708B2 (en) | 2012-05-23 | 2019-03-07 | Kyoto University | Highly efficient method for establishing artificial pluripotent stem cell |
| US20150133467A1 (en) * | 2012-06-06 | 2015-05-14 | Kyoto University | Screening method, protein instability and/or stability inducers, and protein activity assessment |
| CA2895791A1 (en) | 2013-01-25 | 2014-07-31 | Xcell Biosciences, Inc. | Methods, compositions, kits, and systems for selective enrichment of target cells |
| JP6495658B2 (ja) | 2013-02-08 | 2019-04-03 | 国立大学法人京都大学 | 巨核球及び血小板の製造方法 |
| US9738861B2 (en) | 2013-03-06 | 2017-08-22 | Kyoto University | Culture system for pluripotent stem cells and method for subculturing pluripotent stem cells |
| US9790468B2 (en) | 2013-03-15 | 2017-10-17 | Avita Iinternational Ltd. | Multifunctional immature dental pulp stem cells and therapeutic applications |
| JP6473077B2 (ja) | 2013-03-21 | 2019-02-20 | 国立大学法人京都大学 | 神経分化誘導用の多能性幹細胞 |
| EP2980207B1 (en) | 2013-03-25 | 2018-12-05 | Foundation for Biomedical Research and Innovation at Kobe | Cell sorting method |
| JP6461787B2 (ja) | 2013-04-12 | 2019-01-30 | 国立大学法人京都大学 | 肺胞上皮前駆細胞の誘導方法 |
| JP6429280B2 (ja) | 2013-05-14 | 2018-11-28 | 国立大学法人京都大学 | 効率的な心筋細胞の誘導方法 |
| EP3031905A4 (en) | 2013-08-07 | 2017-04-26 | Kyoto University | Method for producing pancreatic hormone-producing cell |
| AU2014316100B2 (en) | 2013-09-05 | 2020-11-05 | Eisai R&D Management Co., Ltd. | New method for inducing dopamine-producing neural precursor cells |
| US10100283B2 (en) | 2013-11-01 | 2018-10-16 | Kyoto University | Efficient chondrocyte induction method |
| FR3016372B1 (fr) * | 2014-01-13 | 2018-01-19 | Centre Leon Berard | Procede de preparation de cellules pluripotentes induites |
| EP3093023B1 (en) * | 2014-01-24 | 2020-11-04 | Quarrymen & Co. Inc. | Pharmaceutical composition for cancer treatment |
| CN104894060B (zh) * | 2014-03-03 | 2018-11-06 | 中国科学院上海生命科学研究院 | 诱导体细胞转分化为神经干细胞的方法及其应用 |
| CA2941004A1 (en) * | 2014-03-04 | 2015-09-11 | Peter Flynn | Improved reprogramming methods and cell culture platforms |
| JP6715482B2 (ja) | 2014-07-18 | 2020-07-01 | 国立大学法人京都大学 | 多能性幹細胞から免疫細胞療法用t細胞を誘導する方法 |
| WO2017017686A1 (en) * | 2015-07-29 | 2017-02-02 | Hadasit Medical Research Services And Development Ltd. | Large scale production of retinal pigment epithelial cells |
| CN117737124A (zh) | 2015-10-16 | 2024-03-22 | 菲特治疗公司 | 用于诱导和维护基态多能性的平台 |
| JP7011260B2 (ja) | 2016-04-22 | 2022-02-10 | 国立大学法人京都大学 | ドーパミン産生神経前駆細胞の製造方法 |
| US20170369904A1 (en) * | 2016-06-22 | 2017-12-28 | Xcell Biosciences, Inc. | Methods for increasing cell culture transfection efficiency and cellular reprogramming |
| CA3042495A1 (en) | 2016-11-10 | 2018-05-17 | Viacyte, Inc. | Pdx1 pancreatic endoderm cells in cell delivery devices and methods thereof |
| US20210254013A1 (en) * | 2018-06-14 | 2021-08-19 | Figene, Llc | Use of fibroblasts and/or modified fibroblasts for three dimensional tissue printing |
| US20210299331A1 (en) | 2018-07-19 | 2021-09-30 | Kyoto University | Pluripotent stem cell-derived plate-shaped cartilage and method for producing the same |
| JP7357369B2 (ja) | 2018-07-23 | 2023-10-06 | 国立大学法人京都大学 | 新規腎前駆細胞マーカーおよびそれを利用した腎前駆細胞の濃縮方法 |
| WO2020130147A1 (ja) | 2018-12-21 | 2020-06-25 | 国立大学法人京都大学 | ルブリシン局在軟骨様組織、その製造方法及びそれを含む関節軟骨損傷治療用組成物 |
| AU2020267768A1 (en) * | 2019-05-09 | 2021-12-23 | Figene, Llc | Fibroblast generated patient-specific vaccines |
| EP3969571A4 (en) | 2019-05-14 | 2023-05-24 | Aleph Farms Ltd. | PLURIPOTENTE CELL AGGREGATES AND THEIR USE |
| JP7058431B2 (ja) | 2019-12-12 | 2022-04-22 | 国立大学法人千葉大学 | 巨核球および血小板を含む凍結乾燥製剤 |
| US20230256024A1 (en) | 2020-07-13 | 2023-08-17 | Kyoto University | Skeletal muscle precursor cells and method for purifying same, composition for treating myogenic diseases, and method for producing cell group containing skeletal muscle precursor cells |
| EP4180514A4 (en) | 2020-07-20 | 2024-09-04 | Aichi Medical University | COMPOSITION FOR UNDIFFERENTIATED MAINTENANCE CULTURE OF PLURIPOTENT CELLS, MEDIUM FOR UNDIFFERENTIATED MAINTENANCE CULTURE OF PLURIPOTENT CELLS, METHOD FOR MAINTENANCE CULTURE IN UNDIFFERENTIATED STATE OF PLURIPOTENT CELLS, AND METHOD FOR PRODUCING PLURIPOTENT CELLS |
| CN118679244A (zh) | 2022-02-09 | 2024-09-20 | 住友制药株式会社 | 判定培养液中的细胞在由多能干细胞向中脑底板区域的神经系统细胞的分化中的分化能力的方法 |
| WO2024248017A1 (ja) | 2023-05-29 | 2024-12-05 | 国立研究開発法人理化学研究所 | 心筋細胞を成熟させる方法及び成熟した心筋細胞の製造方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001096532A2 (en) * | 2000-06-15 | 2001-12-20 | Tanja Dominko | Method of generating pluripotent mammalian cells by fusion of a cytoplast fragment with a karyoplast |
| CN101492676A (zh) * | 2008-09-16 | 2009-07-29 | 中国科学院广州生物医药与健康研究院 | 用脑膜细胞生成诱导的多能性干细胞的方法及其用途 |
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| CA2307807C (en) | 1997-10-23 | 2008-09-02 | Andrea G. Bodnar | Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture |
| AU2003303071B2 (en) | 2002-12-16 | 2009-04-23 | Technion Research & Development Foundation Ltd. | Methods of preparing feeder cells-free, xeno-free human embryonic stem cells and stem cell cultures prepared using same |
| WO2006019366A1 (en) | 2003-03-28 | 2006-02-23 | Wisconsin Alumni Research Foundation | Physiochemical culture conditions for embryonic stem cells |
| AU2005282414C1 (en) | 2004-09-08 | 2011-04-07 | Wisconsin Alumni Research Foundation | Culturing human embryonic stem cells |
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| US8278104B2 (en) | 2005-12-13 | 2012-10-02 | Kyoto University | Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2 |
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| EP2137295B1 (en) | 2007-01-04 | 2018-04-18 | Karl Tryggvason | Use of a composition and method for enabling proliferation of pluripotent stem cells |
| JP5813321B2 (ja) * | 2007-03-23 | 2015-11-17 | ウィスコンシン アラムニ リサーチ ファンデーション | 体細胞の再プログラム化 |
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| EP2336303B1 (en) | 2008-09-08 | 2015-07-15 | Riken | NKT CELL-DERIVED iPS CELLS AND NKT CELLS DERIVED THEREFROM |
| CA2770412C (en) | 2009-08-07 | 2018-09-11 | Kyoto University | Method of efficiently establishing induced pluripotent stem cells |
-
2009
- 2009-07-30 EP EP09803075.2A patent/EP2307539B1/en active Active
- 2009-07-30 CN CN201611216942.9A patent/CN107012123A/zh active Pending
- 2009-07-30 CN CN200980100065A patent/CN101848994A/zh active Pending
- 2009-07-30 KR KR1020107002641A patent/KR101685209B1/ko active Active
- 2009-07-30 WO PCT/JP2009/063906 patent/WO2010013845A1/en not_active Ceased
- 2009-07-30 US US12/672,222 patent/US9528092B2/en active Active
- 2009-07-30 JP JP2010506476A patent/JP5562231B2/ja active Active
- 2009-07-30 CA CA2697621A patent/CA2697621C/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001096532A2 (en) * | 2000-06-15 | 2001-12-20 | Tanja Dominko | Method of generating pluripotent mammalian cells by fusion of a cytoplast fragment with a karyoplast |
| CN101492676A (zh) * | 2008-09-16 | 2009-07-29 | 中国科学院广州生物医药与健康研究院 | 用脑膜细胞生成诱导的多能性干细胞的方法及其用途 |
Non-Patent Citations (2)
| Title |
|---|
| BRIAN KEITH等: "Hypoxia-Inducible Factors,Stem Cells,and Cancer", 《CELL》 * |
| KAZUTOSHI TAKAHASHI等: "Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors", 《CELL》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2697621C (en) | 2017-01-17 |
| CA2697621A1 (en) | 2010-02-04 |
| US20110039338A1 (en) | 2011-02-17 |
| EP2307539A1 (en) | 2011-04-13 |
| KR20110036523A (ko) | 2011-04-07 |
| WO2010013845A1 (en) | 2010-02-04 |
| KR101685209B1 (ko) | 2016-12-09 |
| JP2011529329A (ja) | 2011-12-08 |
| EP2307539B1 (en) | 2014-11-19 |
| JP5562231B2 (ja) | 2014-07-30 |
| CN101848994A (zh) | 2010-09-29 |
| EP2307539A4 (en) | 2012-10-24 |
| US9528092B2 (en) | 2016-12-27 |
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