CN107002144A - 纤维含量和种皮颜色性状的相关qtl的精细定位与验证以及源自黄色种皮(ysc)卡诺拉系yn01‑429及其谱系的用于这些性状的标记辅助选择的snp标记的鉴定 - Google Patents
纤维含量和种皮颜色性状的相关qtl的精细定位与验证以及源自黄色种皮(ysc)卡诺拉系yn01‑429及其谱系的用于这些性状的标记辅助选择的snp标记的鉴定 Download PDFInfo
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Abstract
一种鉴定与卡诺拉中的期望营养性状相关的数量性状基因座的方法包括:分析一个群体的卡诺拉植物或种质的期望营养性状;使用选自SEQ ID NO:1到SEQ ID NO:111的组的至少一个标记来确定卡诺拉植物或种质的基因型;针对与所述标记相关的数量性状基因座(QTL)的存在进行卡诺拉植物或种质的作图;和使所述QTL与所述期望营养性状相关联。一种分离核酸和/或重组核酸包括与数量性状基因座(QTL)相关的序列,其中该QTL与卡诺拉植物或种质中的期望营养性状相关,并且其中该QTL进一步与选自SEQ ID NO:1到SEQ ID NO:111的组的至少一个标记相关。
Description
优先权声明
本申请要求2014年12月18日提交的“基于纤维含量和种皮颜色性状的QTL的精细定位和验证以及用于源自黄色种皮(YSC)卡诺拉系YN01-429及其谱系的这些性状的标记辅助选择的SNP标记的鉴定”的美国临时专利申请序列号62/093,963的申请日的利益,通过提述将此专利完整并入本文。
技术领域
本公开涉及与低纤维含量和YSC性状相关的数量性状基因座(QTL)的精细定位以及用于在甘蓝型油菜(Brassica napus)中的这些性状的标记辅助选择的SNP标记的鉴定。
背景
卡诺拉(Brassica napus L.,2n=4x=38,AACC),由二倍体白菜型油菜(2n=2x=20,AA)和甘蓝(2n=2x=18,CC)形成的异源四倍体,是世界上特别是在中国、加拿大、欧盟和澳大利亚最重要的植物油料作物之一。卡诺拉粕,在破碎和榨油之后剩余的种子部分,大约占卡诺拉种子的55%。
卡诺拉粕由若干成分组成,包括蛋白质、纤维、残油、碳水化合物和抗营养因子。相比于48%蛋白质大豆粕,卡诺拉粕约含有其75%的蛋白质、80%的能量值和300%的粗纤维,以及较高水平的抗营养因子如硫代葡萄糖苷、单宁、植酸、芥子碱和芥酸,而卡诺拉粕作为家畜饲料其出售价格为大豆粕的60%-70%。参见,例如Hickling(2007)Canola mealcompetitive situation and Canola Council of Canada goals,Canola Meal ResearchMeeting,Saskatoon,Canada;Newkirk(2009)Canola meal feed industry guide(第4版)。卡诺拉粕相对较高的纤维含量是其用于单胃动物物种的重要限制因素(Hickling,2007;Newkirk,2009)。由于卡诺拉粕占种子体积的一半,并且对生物柴油的需求可能会使2006年至2015年的油菜种子生产增加67%(Hickling,2007),需要改变卡诺拉粕的组成特性,由此增加其相对于大豆的营养价值。
加拿大农业及农业食品部(AAFC)的科学家已经开发出相比于黑色种皮(BSC)卡诺拉具有具有更薄的种皮、低纤维和高油的低壳比例的黄色种皮(YSC)系(YN86-37、YN90-1016、YN97-262和YN01-429)(Rakow等人,2011)。将来自AAFC系YN01-429的黄色种子卡诺拉粕与芥菜型油菜、白菜型油菜和棕色种子甘蓝型油菜在肉鸡和单胃动物物种中进行比较的饲喂研究证明了YSC甘蓝型油菜系的优点,如较高的蛋白、较低的纤维、增加的氨基酸消化率和可代谢能含量、以及基于料重比的营养改善和能量利用(Hickling,2009;Slominski等人,2010)。
对低纤维含量性状的遗传和稳定性的了解不足、以及与该性状紧密连锁的鲁棒的高通量标记的缺乏对低纤维含量性状的育种造成了很大的障碍。由于异源四倍体、多基因的作用、母本效应和环境影响,低纤维含量性状的遗传是复杂的,并且与该性状紧密连锁的标记的鉴定是非常具有挑战性的。Somers等人(2001)报告了与YSC系YN90-1016的黄色种皮性状的主要基因(pigmentl)相关的八个随机扩增多态性DNA(RAPD)标记的鉴定以及在申请人的低纤维育种计划中使用的YN97-262和YN01-429的低纤维含量来源(Rakow等人,2011)。主要基因解释了种子颜色变异的72.3%,而另外两个似乎为加性的基因则解释了21.5%的颜色变异(Somers等人,2001)。
有人提出,AAFC YSC系YN01-429及其谱系的低纤维含量可能受三个隐性基因的控制(Kubik和Thompson,2009)。目前,较低纤维的卡诺拉系的选择主要基于使用成本和劳动密集型分析方法获得的纤维含量数据,或基于种皮颜色,因为种皮颜色与AAFC YSC系YN97-262和YN01-429中的低纤维高度相关。
公开
本发明的一个特定实施方案包括一种鉴定与卡诺拉中的期望营养性状相关的数量性状基因座的方法。该方法包括分析一个种群的卡诺拉植物或种质的期望营养性状。使用选自SEQ ID NO:1到SEQ ID NO:111的组中的至少一个标记来确定卡诺拉植物或种质的基因型。进行卡诺拉植物或种质的定位以确定是否存在与标记相关的数量性状基因座(QTL)。该QTL与期望的营养性状相关。
另一个实施方案涉及具有与QTL相关的序列的分离核酸和/或重组核酸。该QTL与卡诺拉植物或种质中的期望营养性状相关。该QTL进一步与选自SEQ ID NO:1到SEQ ID NO:111的组的至少一个标记相关。
又另一个实施方案涉及一种选择包含期望的营养性状的卡诺拉植物或种质的方法。所述方法包括检测卡诺拉植物或种质中的与QTL连锁的至少一个标记,所述标记选自SEQ ID NO:1到SEQ ID NO:111的组,其中该QTL与卡诺拉植物或种质中的期望营养性状相关。然后基于该标记的存在来选择卡诺拉植物或种质。
根据下面援引附图展开的若干实施方案的详细说明,能够更清楚地了解前述特征和其他特征。
附图简述
图1显示了在Nex828x YN01-429(YSC)DH系中的白度指数(WI)和%ADF的分布。左图显示了种皮颜色的白度指数(WI)对卡诺拉种子中%ADF的作图。右图显示了DH系中%ADF的分布。括号中的数字表示卡诺拉种子中的%ADF。
图2显示了在DN051493x YN01-429(YDN)DH系中的白度指数(WI)和%ADF的分布。左图显示了针对卡诺拉种子中%ADF绘制的种皮颜色的白度指数(WI)。右图显示了DH系中%ADF的分布。括号中的数字表示卡诺拉种子中的%ADF。
图3A显示了在N09上鉴定的QTL的位置(X轴)和显著性(Y轴上的LOD评分)。图3B显示了在N09上鉴定的QTL的加性效应。
图4A显示了在N11上鉴定的QTL的位置(X轴)和显著性(Y轴上的LOD评分)。图4B显示了在N09上鉴定的QTL的加性效应。
图5A显示了在N09上鉴定的QTL的位置(X轴)和显著性(Y轴上的LOD评分)。图5B显示了在N09上鉴定的QTL的加性效应。
图6A显示了在N11上鉴定的QTL的位置(X轴)和显著性(Y轴上的LOD评分)。图6B显示了在N11上鉴定的QTL的加性效应。
图7显示了以NDN DH群体构建的在N09上的主要ADF QTL区间的图谱。在4.9cM QTL区间之内,鉴定了111个SNP标记,包括侧翼标记DBSNP01120和DBSNP02172。ADF_9和WI_9基因座也定位在该区间内。
图8显示了以YDN、YSC和TN DH群体的N09构建的N09的图谱,显示了在N09上针对基于纤维含量(ADF_9)和种皮颜色(WI_9)的主要QTL的在0.0-4.9cM之内鉴定的18个SNP标记。
公开
本文描述了高通量单核苷酸多态性(SNP)标记和高密度遗传图谱,其用于基于纤维含量和种皮颜色性状的数量性状基因座(QTL)的精细定位和验证。在一些实施方案中,与纤维含量和种皮颜色性状紧密连锁的SNP标记可用于黄色种子卡诺拉(YSC)系中的期望营养性状的标记辅助选择(MAS)。在具体实施方案中,YSC系可以是AAFC YSC系YN01-429及其谱系。
还公开了利用基于来自AAFC YSC系YN01-429的纤维含量和种皮颜色性状的SNP标记和高密度遗传图谱的方法,其中使用一组广泛的两个DH群体的表型数据。在具体实施方案中,描述了两个DH群体中的在N09上的主要QTL,所述主要QTL解释了纤维含量和种皮颜色性状变异的59.2%至74.7%,并且描述了两个DH群体中的在N11上的次要QTL,所述次要QTL解释了纤维含量和种皮颜色性状的1.4%至7.2%的变异。在两个群体中种皮颜色性状(WI和L)与ADF含量之间都存在高度关联(R2=0.67-0.85)。
本文中还公开了N09上相距纤维含量和种皮颜色主要QTL 0.0-4.9cM内的18个SNP标记(参见图8),以及N11上相距纤维含量和种皮颜色次要QTL0.0-4.1cM内的40个SNP标记,这些标记可用于从YSC系YN01-429及其谱系标记辅助选择复合低纤维含量和YSC性状的实施方案中,因此,如果YN01-429或其谱系在育种计划中用作低纤维含量的来源,这些标记可以改进具有低ADF含量的卡诺拉系的育种过程。
开发了一种低纤维产品,其目的是通过增加蛋白质含量(从36%到44%)和真代谢能(TME)(增加16%-20%)并降低纤维含量(从15%-19%降至10%以下)将商业卡诺拉粕的营养价值提高到48%蛋白质大豆粕的价值的85-90%。这些提高尤其是在单胃物种中预计将增加卡诺拉粕的营养价值,并且会增加饮食纳入率。
还开发了相比于黑色种皮(BSC)卡诺拉(Rakow等人,2011)具有较薄种皮、低纤维和高油的低壳比例的YSC系(YN86-37、YN90-1016、YN97-262和YN01-429)。将来自AAFC系YN01-429的黄色种子卡诺拉粕与芥菜型油菜、白菜型油菜和棕色种子甘蓝型油菜在肉鸡和单胃动物物种中进行比较的饲喂研究证明了YSC甘蓝型油菜系的优点,包括较高的蛋白、较低的纤维、增加的氨基酸消化率和可代谢能含量、以及基于料重比的营养改善和能量利用(Hickling,2009;Slominski等人,2010)。
研究了YSC系YN97-262和YN01-419的黄色种子/低纤维性状与ω9脂肪酸谱、以及其他期望的农艺和种子质量属性的组合。
I.YN01-429的低纤维含量和YSC性状的定位和验证
在一个优选实施方案中,本公开描述了使用单核苷酸多态性(SNP)标记来鉴定和定位与甘蓝型油菜中的低纤维含量和黄色种皮(YSC)性状相关的数量性状基因座(QTL)的方法。在实施方案中,定义了YSC系YN01-429中的QTL。在一些实施方案中,所述标记可用于源自YSC系YN01-429及其谱系的低纤维含量和YSC性状的标记辅助选择。
利用SNP标记和高密度遗传图谱,使用两个双单倍体(DH)群体的一组广泛的表型数据,对AAFC YSC系YN01-429的纤维含量和种皮颜色性状进行了精细定位和验证。这些实验更详细概述于实施例1-5中。两个DH群体YSC和YDN,都是从春卡诺拉系杂交开发的。YSC群体的183个DH系是通过AAFC黄色种子/低纤维系YN01-429和DAS Nexera黑色种子/高纤维品种Nex828之间的杂交而开发的,并在2007年与两个亲本一起种植在加拿大AAFCSaskatoon研究农场和DAS Rosthern研究农场的行配对的样地中,以便进行表型分析。在2007年,使用近红外光谱(NIR)ADF(在图3和图4中称为ADF_A),并使用Hunter实验室的种皮颜色白度指数(在图3和图4中称为WI_A)测量,通过AAFC分析来自两个地点的种子样品。还通过使用AOAC参考方法(AOAC官方方法973.18)的在印第安纳波利斯的DAS AnalyticalTechnologies Group的ADF(图3和图4中的ADF_A),并且通过在印第安纳波利斯的DASBioprocessGroup的种皮颜色白度指数(在图3和图4中称为WI_D)和Hunter实验室亮度指数(在图3和图4中称为L)测量,在2011年对该群体进行了分析。通过YN01-429和DAS Nexera黑色种子/高纤维品种DN051493之间进行杂交,开发了YDN群体的400个DH系。2011年在加拿大将YDN群体与两个亲本一起种植在PikeLake和Cudworth,以便进行表型分析。分析种子样品的ADF(在图5和6中称为ADF_M1和ADF_M2)、种皮颜色白度指数(在图5和图6中称为WI)和Hunter实验室亮度指数(在图5和图6中称为L)。除了YSC和YDN群体的纤维含量和种皮颜色性状的QTL定位之外,还添加了来自Tapidor(欧洲冬性栽培种)和Ningyou7(中国半冬性栽培种)之间的杂交的具有181个DH系的第三DH群体(称为TN群体),以进行共有图谱构建;TN群体的纤维含量和种皮颜色性状没有分离。用12,000个SNP标记对这三个DH群体进行基因分型,并用YSC、YDN和TN群体的单独图谱构建共有图谱。使用复合区间作图(CIM)进行全基因组QTL扫描。在QTL定位之后,将基于纤维含量和种皮颜色性状的QTL的在0.0-5.0cM之内的SNP标记转向到KASPar测定,以进行源自YN01-429及其谱系的这些性状的MAS。
在两个群体中,在种皮子颜色性状WI和L之间(R2=0.81-0.99)以及在种皮颜色性状和ADF含量之间(R2=0.66-0.85)也观察到高度关联(图1和图2)。由于主要QTL(R2=59.2%-74.7%)对N09的影响较大,以及DH系中纤维含量和种皮颜色性状的双模态分布(图1和图2),纤维含量和种皮颜色数量性状可以作为质量性状对待。在YSC和YDN群体中,在将ADF和WI的数量性状转化为质量性状之后,ADF(ADF_09)和WI(WI_09)均被定位到N09上的主要QTL所在位置的同一个基因组区域。图7显示了用YSC、YDN和TN群体以及323个SNP标记构建的N09的共有图谱上的主要ADF和WI QTL、ADF_09和WI_09的图谱位置。
Somers等人(2001)和Rakow等人(2011)指明,来自AAFC的YSC系在多个环境中提供了一致的低纤维含量,并且低木质素含量总是与黄色种子的颜色相关。用174个DH和2,982个多态SNP标记构建了YSC遗传图谱,总长度为2,515.8cM,平均长度为0.80cM/标记。用397个DH系和2,972个SNP标记构建了YDN群体,总长度为2,189cM,平均长度为0.74cM/标记。用181个DH系和2,716个多态SNP标记构建了TN遗传图谱,总长度为1905.7cM,平均长度为0.70cM/标记。在实施方案中,本公开描述了用YDN、YSC和TN群体构建的平均值为0.47cM的5,500个SNP标记的共有图谱(图7)。
YSC和YDN群体的纤维含量和种皮颜色性状分离,并用于QTL定位,进一步证实种皮颜色性状和纤维含量性状高度关联(图3-6)。QTL定位表明,针对两个群体中的所有种皮颜色性状(WI和L)和ADF含量,在连锁群(LG)N09上鉴定了主要QTL,并且在LG N11上鉴定了次要QTL(图4-6)。
在YSC群体中,在N09上鉴定的主要QTL解释了ADF变异的71.5%,与以范围40到47之间的LOD评分解释了WI变异的59.2%和亮度指数(L)变异的60.8%的主要QTL一致(图3)。在Nex828x YN01-429群体中,在N11上鉴定的次要QTL解释了ADF变异的2.4%,与以范围5到9之间的LOD评分分别解释了WI变异的7.2%和亮度指数(L)变异的6.3%的次要QTL一致(图4)。
YDN群体的QTL分析进一步证实了YSC群体的结果。在N09上鉴定的主要QTL解释了ADF变异的73.4%,与以LOD评分143分别解释了WI变异的74.0%和亮度指数(L)变异的74.7%的主要QTL一致(图5)。在N11上鉴定的次要QTL解释了ADF变异的1.4%,与以范围3到32之间的LOD评分分别解释了WI变异的5.9%和亮度指数(L)变异的5.7%的次要QTL一致(图6)。这些结果与Somers等人(2001)的结果一致,Somers等人(2001)的结果表明一个主要基因解释了种子颜色变异的72.3%,而另外两个基因似乎是加性并且解释了21.5%的颜色变异。由于种皮颜色QTL(WI和L)与纤维含量QTL完全一致,并且在源于YN01-429的两个DH群体中解释了与ADF含量QTL几乎相同百分比的表型变异(R2=77.4%),如果YSC系YN01-429或其谱系在育种计划中用作低ADF含量的来源,则种皮颜色指数(WI和L)是卡诺拉种子中纤维含量的良好指标。
II.SNP标记以及YN01-429的低纤维含量和YSC性状的相关QTL
在一些实施方案中,本公开描述了一个主要QTL,其解释了两个双单倍体(DH)植物群体中纤维含量和种皮颜色性状的表型变异的59.2%-74.7%。在具体实施方案中,已经发现一个次要QTL解释了两个双单倍体植物群体中的这些性状的表型变异的1.4%-7.2%。公开了在两个群体中的在种皮颜色性状(WI和L)与ADF含量之间的高度关联。
在替代性实施方案中,一组高通量标记与YSC系YN01-419的纤维含量和种皮颜色性状紧密连锁。在其他实施方案中,与QTL连锁的核酸序列与期望的营养性状相关。该序列可源自黄色种皮(YSC)系YN01-429或其谱系。具体实施方案涉及一组为18个的SNP标记,其位于在DH群体的Nex828xYN01-429(YSC)和DN051493x YN01-429(YDN)中鉴定的主要ADF和种皮(WI)QTL的0.0-4.9cM之内。
在具体实施方案中,SNP标记包括图7中标识为DBSNP352222到DBSNP2222111的那些标记,它们被DSNP01120和DSNP02172所侧翼。在另一个实施方案中,SNP标记包括DBSNP357223、DBSNP357224、DBSNP357226、DBSNP357227、DBSNP357228、DBSNP357230、DBSNP357231、DBSNP357233、DBSNP357234、DBSNP357244、DBSNP357247、DBSNP357250、DBSNP357252、DBSNP357253、DBSNP357254、DBSNP357255、DBSNP357256、DBSNP357257、DBSNP357258、DBSNP357273、DBSNP357287、DBSNP357288、DBSNP357290、DBSNP357291、DBSNP357292、DBSNP357293、和DBSNP357294,如图7中所示。
III.YN01-429的低纤维含量和YSC性状的标记辅助选择(MAS)
某些实施方案涉及使用标记辅助选择(MAS)选择卡诺拉植物或种质的与QTL相关的期望营养性状的方法。例如,可以使用YSC系YN01-429。可以使用种子颜色测量代替昂贵且耗时的纤维含量的湿化学分析。公开的在N09上的纤维含量和种皮颜色主要QTL或在N11上的纤维含量和种皮颜色次要QTL的4.9cM之内的SNP标记可用于MAS,并且将极大地加速具有低纤维含量的卡诺拉系的育种,这是DAS低纤维产品概念的最重要构成之一。
具体实施方案描述了在YSC系YN01-429的复合纤维含量和种皮颜色性状的标记辅助选择(MAS)中使用所鉴定的QTL,以促进芸苔属的育种和更有效的期望营养性状的选择的方法。具体实施方案涉及卡诺拉品种的标记辅助选择,以增加卡诺拉粕的营养价值,特别是对于包括单胃动物和反刍动物在内的饲养动物而言的营养价值。
根据某些实施方案,该方法可用于选择平均包含至少约44%的粗蛋白和不超过约14%的以干质量计的通过NIR测定的酸性洗涤纤维的卡诺拉种子或种质。在替代实施方案中,卡诺拉种子或种质可以进一步包含平均至少约49%的粗蛋白含量。在其他实施方案中,卡诺拉种子或种质可包含平均不超过约12%的酸性洗涤纤维含量。另外,通过所公开的方法选择的卡诺拉种子或种质可以进一步包括以下性状:降低的硫代葡萄糖苷含量、低单宁含量和/或低的残留细胞壁含量。
IV.缩写
ADF 酸性洗涤纤维
AME 表观代谢能
DAS 陶氏益农公司
DH 双单倍体
FAME 脂肪酸/脂肪酸甲酯
NMR 核磁共振
NIR 近红外光谱法
QTL 数量性状基因座
RAPD 随机扩增多态性DNA
SNP 单核苷酸多态性
RCW 残留细胞壁
V.术语
异源四倍体:如本文中使用的,“异源四倍体”通常是指具有四倍于单倍体生物的染色体组的杂交生物。
卡诺拉油:卡诺拉油是指从油菜的商业品种中榨取的油。为了生产卡诺拉油,通常在谷物升运器中将种子分级并混合以产生可接受的均匀产品。然后使混合的种子破碎,一般用己烷萃取油,随后进行精制。然后可以销售所得到的油以供使用。含油量一般被测量为占整个干燥种子的百分比,特定的含油量是不同品种卡诺拉的特征。可以使用各种分析技术容易且常规地测定含油量,例如但不限于:NMR;NIR;和索氏萃取法。通常通过从种子中提取油样品,产生存在于油样品中的脂肪酸的甲酯,并使用气相色谱法分析样品中各种脂肪酸的比例来确定总脂肪酸的组成百分比。脂肪酸组成也可以是特定品种的区别特征。
良种系:如本文中使用的,术语“良种系”是指针对优良农艺性能进行育种和选择所产生的任何系。良种植物是来自良种系的任何植物。
强化的卡诺拉粕:如本文中使用的,术语“强化的卡诺拉粕”是指从卡诺拉种子产生的卡诺拉粕,其具有降低的纤维含量,并且可能具有增加的蛋白质和真代谢能含量,以及减少的抗营养因子如硫代葡萄糖苷、单宁、植酸、芥子碱和芥酸。具有一些或所有这些特征的粕可以使动物物种尤其是单胃动物的饮食的包含率增加。
植物系:如本文中使用的,“系”是指一组对于至少一个性状而言在个体间显示很少遗传变异(例如,没有遗传变异)的植物。可通过几个世代的自花授粉和选择,或替代地通过使用组织或细胞培养技术从单个亲本的营养繁殖来建立近交系。如本文中使用的,术语“栽培种”“品种”、和“类型”是同义词,并且这些术语是指用于商业生产的系。
植物材料:如本文中使用的,术语“植物材料”是指全部或部分地从植物衍生的任何加工或未加工的材料。例如但不限于,植物材料可以是植物部分、种子、果实、叶、根、植物组织、植物组织培养物、植物外植体、或植物细胞。
数量性状:如本文中使用的,“数量性状”可以指以不同程度沿着通常连续的梯度表达并且经常与两个或更多个基因连锁并受环境影响的性状或表型。
数量性状基因座:如本文中使用的,“数量性状基因座”是指含有基于数量性状的一个基因或多个基因或与其连锁的DNA的区段或区域。
种子颜色:在一些实施方案中,本公开是指以种子颜色为特征的卡诺拉品种(例如,卡诺拉近交系和杂种)。基于从具有完全的种子成熟度或接近完全的种子成熟度的健康植物获得的种子,通常以1-5级对卡诺拉种子颜色等级或“种子颜色”进行评分。“1”表示完全的黄色。“2”表示主要为黄色,有少许棕色。“3”表示棕色和黄色的混合状态。“4”和“5”分别表示棕色和黑色。白度指数(WI)评分也可用于描述卡诺拉品种。例如,黄色种子系YN97-262和9592分别具有-34.6和-33.2的白度指数得分,并且种子颜色得分为1。黑色种子系Nex715和Nex 705分别具有-0.2和-4.4的白度指数得分,并且种子颜色得分为4。黑色种子系46A65和Q2分别具有0.3和-3.9的白度指数得分,并且种子颜色得分为5。特定种子的颜色也可以描述为与任何这些系相比的百分比或其他比率。
稳定性:如本文中使用的术语“稳定性”或“稳定的”是指通过多个世代维持在基本相同水平的给定植物组分。例如,稳定的组分可以在基本上相同的水平上维持至少三个世代。在这种情况下,术语“基本上相同”在一些实施方案中可以指在两个不同世代之间保持在25%之内;20%之内;15%之内;10%之内;5%之内;3%之内;2%之内;和/或1%之内的组分,以及在两个不同世代之间完全保持的组分。在一些实施方案中,稳定的植物组分可以是,例如但不限于,油组分;蛋白质组分;纤维组分;色素组分;硫代葡萄糖苷组分;和木质素组分。组分的稳定性可能受到一个或多个环境因素的影响。例如,油组分的稳定性可能受到例如但不限于:温度、位置、胁迫、和种植时间的影响。在田间条件下具有稳定组分的植物的后代将预期以类似的方式产生例如如上所述的植物组分。
性状或表型:术语“性状”和“表型”在本文中可互换使用。出于本公开的目的,特别感兴趣的性状是低纤维含量和种皮颜色。一些卡诺拉品种表现出黄色种皮,而另外的品种表现出暗色(例如,黑色、深色和杂色)种皮。
“品种”或“栽培种”是用于商业生产的植物系,其在繁殖时的特征是不同的、稳定的和一致的。
除非另外指明,本文中使用的术语“一个/一种”(“a”)和“一个/一种”(“an”)是指至少一个/一种。
本文中引用的所有参考文献(包括出版物、专利、和专利申请)通过提述将其并入本文,其程度不与本公开的明确细节相冲突,并入的程度如同每篇参考文献被单独地并且明确地指示通过提述并入,并且在本文中以其整体进行阐述。提供在本文中论述的参考文献只不过是因为它们的公开在本申请的申请日之前。在本文中不应被解释为承认本发明人无权凭借在先发明而先于这样的公开。
实施例
提供以下实施例以展示某些具体特征和/或方面。这些实施例不应被理解为将本公开限于所描述的具体特征或方面。
实施例1:植物材料和DNA提取
为了对来自黄色种皮(YSC)系YN01-429的低纤维含量和种皮颜色性状进行精细定位和验证,分别在2007年和2010年通过春卡诺拉系的杂交开发了两个双单倍体(DH)群体YSC和YDN。通过加拿大农业及农业食品部(AAFC)黄色种子低纤维系YN01-429和DAS Nexera黑色种子高纤维品种Nex828之间的杂交,开发了YSC群体的176个DH系。通过YN01-429和DASNexera黑色种子高纤维品种DN051493之间进行杂交,开发了YDN群体的399个DH系。DH群体TN来源于欧洲冬季栽培种Tapidor和中国半冬栽培种Ningyou7之间的杂交。这个群体是被国际卡诺拉研究界广泛用于性状作图和基因组学研究的参考作图群体(Shi等人,2009),并且其纤维含量和种皮颜色性状没有分离。它与YSC和YDN群体一起用于共有图谱构建,以鉴定与纤维含量和种皮颜色性状紧密连锁的更多单核苷酸多态性(SNP)标记。
使用DAS Biocel提取法从每个样品的8个叶圆片(1eaf punch)提取所述群体的基因组DNA(Bohl等人,2009)。利用制造商的说明书通过Quant-iTTM 定量试剂盒(Invitrogen,Carlsbad,CA),或根据制造商的说明通过Nanodrop 8000分光光度计(ThermoScientific,Waltham,MA)将DNA样品定量。
实施例2:表型数据
2007年在加拿大将来自YSC群体的176个DH系与作为对照的两个亲本一起种植在AAFC Saskatoon研究农场和DAS Rosthern研究农场的行配对的样地中,以便进行表型分析。从两个地点收集来自所有已建立样地的种子样品,并由AAFC使用近红外光谱法(NIR)分析酸洗涤纤维(ADF)和种皮颜色白度指数(WI)。在2007年使用AOAC参考方法(AOAC官方方法973.18)还分析了该群体的ADF,并且在2011年分析了种皮颜色白度指数和Hunter实验室亮度指数(L)。
2011年在加拿大将YDN群体与作为对照的两个亲本一起种植在Pike Lake和Cudworth的行配对的样地中,以进行表型分析。2011年使用两个NIR模型分析来自361个DH系的种子样品的ADF,并且分析其种皮颜色白度指数和Hunter实验室亮度指数。
对于两个群体,观察到在两个作图亲本之间的纤维含量和种皮颜色的显著差异,如表1所示。DH系中纤维含量的分布揭示了两个群体中偏向较高纤维范围的双模态分布(图1和图2)。在两个群体中,在种皮子颜色性状WI和L之间(R2=0.81-0.99)以及在种皮颜色性状和纤维含量性状之间(R2=0.66-0.85)观察到高度关联(图1和图2)。
表1:YSC和YDN群体中的亲本和DH系的%ADF和种皮颜色数据WI和L。
实施例3:基因型数据
根据制造商的方案(Illumina,San Diego,CA),利用在DAS开发的12,000个SNP标记在BeadStation 500G上的两个Illumina Infinium芯片上将三个DH群体YSC、YDN、和TN进行基因分型。使用GenomeStudio Genotyping Analysis Module v1.8.4(Illumina,SanDiego,CA)分析基因型数据,其将每个SNP的荧光信号转换为A和B信号,所述信号的值反映任意分配的A和B等位基因的相对丰度。使用强度R的曼哈顿距离度量将信号转化为极坐标,其中θ∈[0,1]代表角度∈[0,90]度。将每个标记簇集在Cartesian坐标系中,并将基因型{AA,AB,BB}指定给接近于θ={0,1/2,1}的簇中的样品。
实施例4:连锁图谱和共有图谱构建
使用MAPMAKER/EXP 3.0(Lander等人1987;Lincoln等人,1992)以LOD评分10.0和Haldane作图函数构建了YSC、YDN和TN群体的各个图谱,用Phenomap Enterprise 3.0(GeneFlow Inc.,Centreville,VA)构建了共有图谱。
用176个DH系和2,982个多态SNP标记构建了YSC遗传图谱,其具有总长度2,515.8cM,平均长度为0.80cM/标记。用399个DH系和2,972个SNP标记构建了YDN遗传图谱,其具有总长度2,189cM,平均长度为0.74cM/标记。用181个DH系和2,716个多态SNP标记构建了TN遗传图谱,其具有总长度1905.7cM,平均长度为0.70cM/标记。用YDN、YSC和TN群体构建了5,500个SNP标记的共有图谱。
实施例5:QTL定位
使用在QTL Cartographer V2.5(Wang等人,2011)中实施的复合区间作图(CIM)进行QTL定位。使用LOD评分3.0作为阈值,鉴定显著影响种皮颜色和纤维含量性状的基因组区域。
表2显示了针对种皮颜色和纤维含量鉴定的QTL的详情。结果表明,针对两个群体YSC和YDN中的种皮颜色性状WI和L以及纤维含量性状ADF,在连锁群(LG)N09上鉴定了主要QTL,并且在N11上鉴定了次要QTL。在YSC群体中,在N09上针对ADF鉴定的主要QTL与针对LOD评分范围为40到47的WI和L的主要QTL一致。在YSC群体中,在N11上针对ADF鉴定的次要QTL与针对LOD评分范围为3到9的WI和L的次要QTL一致。YDN群体的QTL分析进一步证实了YSC群体的结果。对于YDN群体,在N09上针对ADF鉴定的主要QTL与针对LOD评分范围为136到143的WI和L的主要QTL一致。在N11上针对ADF鉴定的次要QTL与针对LOD评分范围为7到26的WI和L的次要QTL一致。
表2:在LOD评分≥3的YSC和YDN群体中通过基于ADF含量和种皮颜色性状(WI和L)的显著性QTL解释的表型变异(R2)。
实施例6:作为质量性状的ADF和种皮颜色的定位
由于主要QTL(R2=59.2%-74.7%)对N09的影响较大,以及DH系中纤维含量和种皮颜色性状的双模态分布(图1和图2),纤维含量和种皮颜色数量性状可以被视为质量性状。根据其ADF含量和种皮颜色WI,将两个群体的DH系分为两组,一组具有低纤维/高WI和纯合的YN01-429等位基因,另一组具有高纤维/低WI和纯合的Nex828或DN051493等位基因(图1和图2)。
在YSC群体中,具有YN01-429等位基因的DH系在卡诺拉种子中具有10.0±1.0%的平均ADF,具有Nex828等位基因的DH系具有14.0±1.6%的平均ADF(图1)。在YDN群体中,具有YN01-429等位基因的DH系在卡诺拉种子中具有12.8±0.9%的平均ADF,具有DN051493等位基因的DH系具有18.5±1.4%的平均ADF(图2)。在将ADF和WI的数量性状转化为质量性状之后,ADF(ADF_09)和WI(WI_09)被映射到YSC和YDN群体中N09上主要QTL所在位置的同一个基因组区域。图7显示了主要ADF和WIQTL、ADF_09和WI_09在N09的YDN群体图谱上的图谱位置。
实施例7:N09上主要QTL的精细定位
YDN群体随后用于N09上主要QTL的精细定位和验证。用1387个SNP构建了N09的新遗传图谱。侧翼标记DBSNP01120和DBSNP02172定义了4.9cM的QTL区间,对应于通过工业联盟在AAFC测序的甘蓝型油菜参考基因组DH12075上的0.46Mb。N09上的主要QTL具有75%的R2。利用来自育种计划的多个DAS专有DH群体,在QTL区间之内的标记的盲法筛选证实,标记预测的表型与实际表型之间的一致性≥98%。图3显示了ADF QTL区间连同ADF_09和WI_09基因座的遗传图谱。
在N09上的4.9cM QTL区间之内,鉴定了111个SNP标记,包括侧翼标记DBSNP01120和DBSNP02172。表3列出了SNP、其以cM表示的遗传位置、YN01-429等位基因以及在甘蓝型油菜参考基因组(DH12075)上SNP的物理位置。
在被DBSNP01120和DBSNP02172所侧翼的4.9cm ADF QTL区间之内定义了一个子区间,其中的标记最适合用于标记辅助选择。侧翼标记DBSNP35722和DBSNP222211定义了一个对供体YN01-429独特的0.5cM的子区间单倍型区域。可使用DBSNP 35722和DBSNP 222211侧翼标记和在子区间内的标记进行标记辅助选择,以便在增强ACM属性的育种时追踪N09上的QTL。
序列表
<110> 美国陶氏益农公司
S·唐
V·里普利
T·G·帕特森
M·威金斯
J·弗卢克
C·奥克森费尔德
D·加西亚
S·M·里兹维
M·塔希尔
R·普罗伊斯
D·科涅威尔
S·隆恩斯雷
Z·埃勒特
K·帕勒门特
<120> 纤维含量和种皮颜色性状的相关QTL的精细定位与验证以及源自黄色种皮(YSC)卡诺拉系YN01-429及其谱系的用于这些性状的标记辅助选择的SNP标记的鉴定
<130> 72430-US-NP
<160> 111
<210> 1
<211> 532
<212> DNA
<213> Brassica napus
<220>
<221> misc_feature
<222> (295)..(295)
<223> 其中n为a、t、c或g
<220>
<221> misc_feature
<222> (369)..(369)
<223> 其中n为a、t、c或g
<220>
<221> misc_feature
<222> (441)..(441)
<223> 其中n为a、t、c或g
<220>
<221> misc_feature
<222> (452)..(452)
<223> 其中n为a、t、c或g
<220>
<221> misc_feature
<222> (473)..(473)
<223> 其中n为a、t、c或g
<400> 1
gaatttccgg gtcgacatca tcgaggccgg gttccccgcc gcgtccaaag acgacttcga 60
ggcggtcaag accatttccg aaaccgtcgg aaacgccgtc gacgagaacg gttacgtccc 120
cgtcatctgc ggtctctcga ggtgcaacga gagagatatc cagacggctt gggaggctgt 180
gagatacgcc aaaaggccta ggatccatac gttcatcgcc acgagtgata ttcacttgga 240
gtataagctc aagaagagta aacaagaagt catcgagatc gckaggagca tggtntaggt 300
tcgctaggag cttggggtgt gatgacgtgg agtttagtcc tgaagatgca ggaaggtcgg 360
agagagagnt ttttgtatga gattcttgga gaagtgataa aagctggagc gacgacactt 420
aatattcctg atactgttgg ntatcacttt gncctagtga gtttggtcag ttngattgct 480
gatataaagg ctaatacccc tgggattgag aacgttgtca tctcgactca tt 532
<210> 2
<211> 1001
<212> DNA
<213> Brassica napus
<400> 2
ctccagcttt tatcacttct ccaagaatct catacaaaaa ctctctctcc gacctgttaa 60
acaaattaaa ataaatataa acagttacag agaaagtgaa agcttggtaa tcaagattag 120
aactaattaa aataaataaa agataattaa accccccacc ttcctgcatc ttcaggacta 180
aactccacgt catcacaccc caagctccta gcgaacctaa ccatgctcct cgcgatctcg 240
atgacttctt gtttactctt cttgagctta tactccaagt gaatatcact cgtggcgatg 300
aacgtatgga tcctaggcct tttggcgtat ctcacagcct cccaagccgt ctggatatct 360
ctctcgttgc acctcgagag accgcagatg acggggacgt aaccgttctc gtcgacggcg 420
tttccgacgg tttcggaaat ggtcttgacc gcctcgaagt cgtctttgga cgcggcgggg 480
aacccggcct cgatgatgtc sacgccgagc ttcgcgagct gccgcgcgat gtcgagcttc 540
tccttggagg tgagggtggc gccgggggac tgctcgccgt cgcggagcgt ggtgtcgaag 600
atgcggacgt agttggggtc ggaaatgcgg ttggggatgt agtccgggcg gcggcggcgg 660
agggggtggg gagggagagg gggcggtgga tctgagatgg agcaggagag gcggagggag 720
gcggaggagg agcggcggcg gtggtgggat ggtgggaaac ggaaggagag tggtgtggtg 780
attgttgtgg agaaggtggg gagagaaggt gttgttgttg atgatgagag tgaagggttt 840
ctgagaaggg aagacgccat tggagacgat tgtgagaaga atggtaaacc taaagagaga 900
gagagatgaa ggtttgaacg tggcggcggc agctacttgg ttaagctcta tctgttcgtt 960
cgtgtcactc ttctctttat ttgacaaaaa caaatctttt t 1001
<210> 3
<211> 1001
<212> DNA
<213> Brassica napus
<400> 3
cttcttgttt actcttcttg agcttatact ccaagtgaat atcactcgtg gcgatgaacg 60
tatggatcct aggccttttg gcgtatctca cagcctccca agccgtctgg atatctctct 120
cgttgcacct cgagagaccg cagatgacgg ggacgtaacc gttctcgtcg acggcgtttc 180
cgacggtttc ggaaatggtc ttgaccgcct cgaagtcgtc tttggacgcg gcggggaacc 240
cggcctcgat gatgtcgacg ccgagcttcg cgagctgccg cgcgatgtcg agcttctcct 300
tggaggtgag ggtggcgccg ggggactgct cgccgtcgcg gagcgtggtg tcgaagatgc 360
ggacgtagtt ggggtcggaa atgcggttgg ggatgtagtc cgggcggcgg cggcggaggg 420
ggtggggagg gagagggggc ggtggatctg agatggagca ggagaggcgg agggaggcgg 480
aggaggagcg gcggcggtgg kgggatggtg ggaaacggaa ggagagtggt gtggtgattg 540
ttgtggagaa ggtggggaga gaaggtgttg ttgttgatga tgagagtgaa gggtttctga 600
gaagggaaga cgccattgga gacgattgtg agaagaatgg taaacctaaa gagagagaga 660
gatgaaggtt tgaacgtggc ggcggcagct acttggttaa gctctatctg ttcgttcgtg 720
tcactcttct ctttatttga caaaaacaaa tctttttttt tggtcccact tgaatattct 780
ccacttaaaa aaatgagtac gacaactgtg ttatacttta aacggcgtcg ttataggata 840
caatagaaaa agtcgaccgg caacgataag gacgatgagt cgattgaaca gtttagaaag 900
gacgtagaac catgagattc accaataagc attgaacaag aagacatgga gatggaaagt 960
tgttaaaaca ttttttaaat gaacttaaca tgtcacattg t 1001
<210> 4
<211> 301
<212> DNA
<213> Brassica napus
<400> 4
caaracattt tcacatatat cacttycatg ctcacytcca ccaaccacaa aaaaatgacg 60
agtactataa agcaagaagg acaaagcata cccaatattt ataaataaat cccaccagct 120
gagtgcatct acatcgcctg agtattaaaa mataaaacaa ggagggccga taagaaggaa 180
gggaacgcaa taacattatc tatgaagata agacttcaga aggcagagag accaagtaag 240
aaaattatgt aggcaagcat tcaagagata caacattacc agtaagctty aggaggataa 300
a 301
<210> 5
<211> 1001
<212> DNA
<213> Brassica napus
<400> 5
tagcatgccc agtagtagca aaagaagtga gcacctaaca cgaaaaaacg ccaaagtcat 60
gctgctttca caagagcagg tagaaaacaa aagaaaaggg aatatacagg attcttagcc 120
atgaacaatg cctggaactt tgtatttttc aaacattagc tctgccgccc tgtcaagcat 180
acaatttgag aacatcagta acacactcaa agatgaacta acataagaca aaagtaaata 240
tctataagca gataaatcag acttctctct atgctgttga gtgtttaaag gaggctcagc 300
tagcagcatg ggctgctcct taggatcaat catcaaacaa ctccttcaga cataaaaaaa 360
catattagag atgacatcaa acaataattt atgagaaaaa actaaagctt tatacagttt 420
acctgaatgc atgttcccat ttttgtttat taaaatattg caaaaataat tgactaaatc 480
ttcatataca aaattttgac kgaaaactgc agctttcatc ttcttcctgg agatttaggt 540
ttatatctcc aattggattg gatttgttct cttgaaggga ttaaaacgaa aaagagggaa 600
aacaaaataa aacgccgttg ccggggatcg aacccgggtc acccgcgtga caggcgtgaa 660
tacttaccac tatactacaa cgactcagtt gattaaagat tcaatcaaaa tatactgaaa 720
taaaaaagtt tacgctttga aatgggagac agagctcaac gtacgcactc acgagattct 780
ccagcctcgc aaccaactcc atcagtagcg aagcgaatca aaatttactg aaataaaaaa 840
aaggttacac tttgaaactt actttctaag taattgacaa tacaatatac aaagaaatgg 900
catattaaag tctctttatc gctgtcttaa tcttcttggt tccactcttg ctccattcag 960
ctagtcagct ctcgcccaag gtccgagttg taacggcccg g 1001
<210> 6
<211> 1001
<212> DNA
<213> Brassica napus
<400> 6
ctttgccttt gtcttattat ttgatcttct ttacagctgt gtacgagtca gctaaacatt 60
gctccgttac tcctctccag tattaaatca tgccaggcca gcgcaagatt gttatttgac 120
tacttttcct tccaattacg ttggattact tcttgatttt agttgtgccg caagatctca 180
gatttctaaa ctctgatgca tcaccttctc ctctattggg tttaaaagag ttaattaacc 240
agaagatcaa acatataatt tggatttgtg tttgttttga ccataaatat gttgaaagca 300
gcgtacatgc tactgctttt atctgggaat ggtgttagct taatatggaa agcgatcatg 360
attaggagca accttagacc ctatattttc tacacatggc tgaaggtgat atggtacgag 420
ataatataat tctatttatt tgtagttaat acccttctac atatatttga tgcagaatca 480
tcaaaccgta actctcccgc rgtaatttga aggccatgga aagtaatgtt tcttttactc 540
attgtaactt aatcatatgc tcttttcatg ttcgtcacat ctcacaactt aagaaatcgt 600
tgctacagtt cttcactttt ttccttgtgg taaagtatat ttttactatt ctttcataca 660
tctctttgta gttaaagtag caagagaatt atgagtctga tgtaggatac aagaggtatc 720
cccaaagaag attaactgcc caaaaacaca tgatatctac taaccagcct ctaattacca 780
aagtagtatc tttttctaat gcatatatac attgattttt atctatgtag gtttagtcac 840
tatatatata ggaggtgcga ctagccatca tttgaattta ttcccactct cattgcagtt 900
tgatcactgg tcagaaatgg gacggttaca cgatctgatt ggtagtagag gcacgcgcag 960
gggggggggg ggtacactta cagccatagt cggagaagtc t 1001
<210> 7
<211> 301
<212> DNA
<213> Brassica napus
<400> 7
aacatcagca agaaggtaac ttgtacacaa ttgaggttta tgttctggta ctttcggtta 60
ggtttgcact tggtgggcaa gaaattgtgg cttttgacct ggaattagtg tgtcacaata 120
ggcaaacaac ttcagtgacc catcttaaac rtaatgctaa gaagcagtgt atttgtttcg 180
tgcttttgaa gtttgaatat attttttctt tctccttttt attaccacaa actactctta 240
tttcctggtt aaaagataaa acgtatggga agcctggtcg gtcttactga gtcattatgc 300
a 301
<210> 8
<211> 1001
<212> DNA
<213> Brassica napus
<220>
<221> misc_feature
<222> (501)..(501)
<223> 其中n为插入/缺失 [-/A]
<400> 8
caagttcaat gagaagggaa aaaaataaca gcgttaacac accttaaaag tattatcatc 60
aggaaacatc tcaagagcct gacccctggt cacttcaatc ctttcaaatg gatgtccttc 120
ctaagatgtc agaggaataa actcaaagct ttgtaaaaga agcaaagaaa tatactgtaa 180
ggagaaatac agagatatca aaaataaaga tatgacaaga ctgaataaga agcagtactc 240
gagcatcccc tgcttcagtt tcgggaaagt gttgctcgtt taaacccaga tcgccataga 300
atgcatcgta gaagaaaccc tggatagaca accaaacaaa taatagagag gcaagcacaa 360
agacacagac aaaaagaatc gctgcaggct cttagtctta cctcatctct agctttgcag 420
ggtccaacgc acagcttaca accatactcc tgttcgagaa ccatggtcaa tgtttaccac 480
aatcaagacg agataaaaaa ngtgttttta ccagggcgag aatgtgagcg ctagaacgcc 540
agaaagtatc acggcctttg tcgctgtcaa aactgaaaaa ctcaagcgaa caatcagctt 600
ccagtggcct attcatgtcc cagaggacat cgtttaccga agagatcagc gctgagtttg 660
ccaatcccac agaaatttgc ctcgcgattt ctgctggagt cgtctcccat ctcttccctt 720
ctttcacgtt tccaccatct cgaattgtaa ccctaattaa gttcacagca gaacaacatt 780
actgtagccc tagctacgca aagtatatat cagcatctat tgtgtttact tgatcggctc 840
gtgtggccga gactgaatct ccgcgagctg cttcgcttgg aactcctcga agagcctgat 900
acgcttctcg atgacggctg agagataagc ctcgtccctg ggatgttcat ccgccatcgt 960
tggacttgga ggagcacaga aagtgacgaa aggccaaggg a 1001
<210> 9
<211> 1001
<212> DNA
<213> Brassica napus
<400> 9
ttcaatgaga agggaaaaaa ataacagcgt taacacacct taaaagtatt atcatcagga 60
aacatctcaa gagcctgacc cctggtcact tcaatccttt caaatggatg tccttcctaa 120
gatgtcagag gaataaactc aaagctttgt aaaagaagca aagaaatata ctgtaaggag 180
aaatacagag atatcaaaaa taaagatatg acaagactga ataagaagca gtactcgagc 240
atcccctgct tcagtttcgg gaaagtgttg ctcgtttaaa cccagatcgc catagaatgc 300
atcgtagaag aaaccctgga tagacaacca aacaaataat agagaggcaa gcacaaagac 360
acagacaaaa agaatcgctg caggctctta gtcttacctc atctctagct ttgcagggtc 420
caacgcacag cttacaacca tactcctgtt cgagaaccat ggtcaatgtt taccacaatc 480
aagacgagat aaaaaaagtg kttttaccag ggcgagaatg tgagcgctag aacgccagaa 540
agtatcacgg cctttgtcgc tgtcaaaact gaaaaactca agcgaacaat cagcttccag 600
tggcctattc atgtcccaga ggacatcgtt taccgaagag atcagcgctg agtttgccaa 660
tcccacagaa atttgcctcg cgatttctgc tggagtcgtc tcccatctct tcccttcttt 720
cacgtttcca ccatctcgaa ttgtaaccct aattaagttc acagcagaac aacattactg 780
tagccctagc tacgcaaagt atatatcagc atctattgtg tttacttgat cggctcgtgt 840
ggccgagact gaatctccgc gagctgcttc gcttggaact cctcgaagag cctgatacgc 900
ttctcgatga cggctgagag ataagcctcg tccctgggat gttcatccgc catcgttgga 960
cttggaggag cacagaaagt gacgaaaggc caagggatga t 1001
<210> 10
<211> 1002
<212> DNA
<213> Brassica napus
<220>
<221> misc_feature
<222> (502)..(502)
<223> 其中n为插入/缺失 [+/ATCACGCACCTGCAAATGT]
<400> 10
ataacagcgt taacacacct taaaagtatt atcatcagga aacatctcaa gagcctgacc 60
cctggtcact tcaatccttt caaatggatg tccttcctaa gatgtcagag gaataaactc 120
aaagctttgt aaaagaagca aagaaatata ctgtaaggag aaatacagag atatcaaaaa 180
taaagatatg acaagactga ataagaagca gtactcgagc atcccctgct tcagtttcgg 240
gaaagtgttg ctcgtttaaa cccagatcgc catagaatgc atcgtagaag aaaccctgga 300
tagacaacca aacaaataat agagaggcaa gcacaaagac acagacaaaa agaatcgctg 360
caggctctta gtcttacctc atctctagct ttgcagggtc caacgcacag cttacaacca 420
tactcctgtt cgagaaccat ggtcaatgtt taccacaatc aagacgagat aaaaaaagtg 480
tttttaccag ggcgagaatg tngagcgcta gaacgccaga aagtatcacg gcctttgtcg 540
ctgtcaaaac tgaaaaactc aagcgaacaa tcagcttcca gtggcctatt catgtcccag 600
aggacatcgt ttaccgaaga gatcagcgct gagtttgcca atcccacaga aatttgcctc 660
gcgatttctg ctggagtcgt ctcccatctc ttcccttctt tcacgtttcc accatctcga 720
attgtaaccc taattaagtt cacagcagaa caacattact gtagccctag ctacgcaaag 780
tatatatcag catctattgt gtttacttga tcggctcgtg tggccgagac tgaatctccg 840
cgagctgctt cgcttggaac tcctcgaaga gcctgatacg cttctcgatg acggctgaga 900
gataagcctc gtccctggga tgttcatccg ccatcgttgg acttggagga gcacagaaag 960
tgacgaaagg ccaagggatg attttttaaa cgacgaatga ga 1002
<210> 11
<211> 1001
<212> DNA
<213> Brassica napus
<400> 11
caaagctttg taaaagaagc aaagaaatat actgtaagga gaaatacaga gatatcaaaa 60
ataaagatat gacaagactg aataagaagc agtactcgag catcccctgc ttcagtttcg 120
ggaaagtgtt gctcgtttaa acccagatcg ccatagaatg catcgtagaa gaaaccctgg 180
atagacaacc aaacaaataa tagagaggca agcacaaaga cacagacaaa aagaatcgct 240
gcaggctctt agtcttacct catctctagc tttgcagggt ccaacgcaca gcttacaacc 300
atactcctgt tcgagaacca tggtcaatgt ttaccacaat caagacgaga taaaaaaagt 360
gtttttacca gggcgagaat gtgagcgcta gaacgccaga aagtatcacg gcctttgtcg 420
ctgtcaaaac tgaaaaactc aagcgaacaa tcagcttcca gtggcctatt catgtcccag 480
aggacatcgt ttaccgaaga ratcagcgct gagtttgcca atcccacaga aatttgcctc 540
gcgatttctg ctggagtcgt ctcccatctc ttcccttctt tcacgtttcc accatctcga 600
attgtaaccc taattaagtt cacagcagaa caacattact gtagccctag ctacgcaaag 660
tatatatcag catctattgt gtttacttga tcggctcgtg tggccgagac tgaatctccg 720
cgagctgctt cgcttggaac tcctcgaaga gcctgatacg cttctcgatg acggctgaga 780
gataagcctc gtccctggga tgttcatccg ccatcgttgg acttggagga gcacagaaag 840
tgacgaaagg ccaagggatg attttttaaa cgacgaatga gagcagacgg gacgatttta 900
tgacaccact ttaccaaagt ttttagtatt taagattttt ttcaaaaaaa aaaaaaagaa 960
tttaattaat ttgcctattt tcacagattt aattcctttg c 1001
<210> 12
<211> 1001
<212> DNA
<213> Brassica napus
<400> 12
atagacaacc aaacaaataa tagagaggca agcacaaaga cacagacaaa aagaatcgct 60
gcaggctctt agtcttacct catctctagc tttgcagggt ccaacgcaca gcttacaacc 120
atactcctgt tcgagaacca tggtcaatgt ttaccacaat caagacgaga taaaaaaagt 180
gtttttacca gggcgagaat gtgagcgcta gaacgccaga aagtatcacg gcctttgtcg 240
ctgtcaaaac tgaaaaactc aagcgaacaa tcagcttcca gtggcctatt catgtcccag 300
aggacatcgt ttaccgaaga gatcagcgct gagtttgcca atcccacaga aatttgcctc 360
gcgatttctg ctggagtcgt ctcccatctc ttcccttctt tcacgtttcc accatctcga 420
attgtaaccc taattaagtt cacagcagaa caacattact gtagccctag ctacgcaaag 480
tatatatcag catctattgt rtttacttga tcggctcgtg tggccgagac tgaatctccg 540
cgagctgctt cgcttggaac tcctcgaaga gcctgatacg cttctcgatg acggctgaga 600
gataagcctc gtccctggga tgttcatccg ccatcgttgg acttggagga gcacagaaag 660
tgacgaaagg ccaagggatg attttttaaa cgacgaatga gagcagacgg gacgatttta 720
tgacaccact ttaccaaagt ttttagtatt taagattttt ttcaaaaaaa aaaaaaagaa 780
tttaattaat ttgcctattt tcacagattt aattcctttg ctactacaga tttgttgttt 840
cttttcttta attctaattc atttacatgt atactagatt cgttttccgc gctacgcgcg 900
gattacatga ttcaaatttg ttaatttaca aaaaatttca ctacatttac aatattacta 960
attgtttata aaacatttta aaacacaata attttatagt t 1001
<210> 13
<211> 1001
<212> DNA
<213> Brassica napus
<400> 13
gaagattttt cgaaagaatt ttttcagctt tttttttttt tttttttggg gtttatgggg 60
aattcttctt tggtttcatt tgttttatat tttgattttc aggcgtctga atacctgggt 120
tgttggatta agcttgaaag aaaaagtaaa cttgagaaat aagttaactt ctaatcctac 180
tgagttatgg tttaaaggat tagaaaatat ccgaataaat tatcctaatg aatttaggat 240
taagaaaaag gaaatatgtg ttttctaatg agtttaggaa atttgattta tatataagga 300
gatgcaaggg tgttgcataa cttatgagtt ttgtgattgt gtgagagctt gaggtttttg 360
agtgagtttt cctcaagaga ttaataagag agttattctt attatagagt ttatacaatt 420
cgagattcta tatgggtatg gaatcgctcg tggaactcac cacccagtca ttggcaaatt 480
atctcaaagg caagaatccc stcaccattc gatctttgtg gaaggtggaa ggcgacctca 540
ctgctgagga ggaagctaag gcgttggcga tgggcgtggc gaaattagga cattaagtcc 600
attgatgctc tacaaaatct tatgtgatta ctgaagtctg aagaagtttg tccaagtgtc 660
gtttgtttga agtcaaaaat aaagatgtag caggattatc aagttctgat cattaaaagt 720
cctattataa tttctatgtt tcatcatcac tttgaagttc agttaatcaa aagtacgatt 780
caagaatatt ccagtactgt ttctcgatcc attattacca aaaagtttag ctaattatct 840
tcctggaaac ttcttctgtt ccccccatag agaaagttgt cctgccttta gttccagatt 900
aaataagatg agtatcaagt acccatatgt attttcttcc aaaatataag aacataatat 960
ccaactataa tttaagaaaa aacaaagatt agtggagaac g 1001
<210> 14
<211> 1001
<212> DNA
<213> Brassica napus
<400> 14
tttgtgattg tgtgagagct tgaggttttt gagtgagttt tcctcaagag attaataaga 60
gagttattct tattatagag tttatacaat tcgagattct atatgggtat ggaatcgctc 120
gtggaactca ccacccagtc attggcaaat tatctcaaag gcaagaatcc cctcaccatt 180
cgatctttgt ggaaggtgga aggcgacctc actgctgagg aggaagctaa ggcgttggcg 240
atgggcgtgg cgaaattagg acattaagtc cattgatgct ctacaaaatc ttatgtgatt 300
actgaagtct gaagaagttt gtccaagtgt cgtttgtttg aagtcaaaaa taaagatgta 360
gcaggattat caagttctga tcattaaaag tcctattata atttctatgt ttcatcatca 420
ctttgaagtt cagttaatca aaagtacgat tcaagaatat tccagtactg tttctcgatc 480
cattattacc aaaaagttta kctaattatc ttcctggaaa cttcttctgt tccccccata 540
gagaaagttg tcctgccttt agttccagat taaataagat gagtatcaag tacccatatg 600
tattttcttc caaaatataa gaacataata tccaactata atttaagaaa aaacaaagat 660
tagtggagaa cgttaaaaaa tactcttata taaaagttta atatatttta tgaatattta 720
aattttagtt ttttttaaaa aaaaagtctc aaaatcaatg acagagaggg tgacattaaa 780
ttaattaatc tttctttatt tggcctgaga tgcatgctgc ttataatagt tagttgcttc 840
cagaggaaac acatattcaa acagacaaga ttagctacga cagttgcctg gtaatatttt 900
ttattttatt aggcttcgtt tggaatgatt attataattt ggtatatgat agagagcttg 960
ggctgtgttc tcacattatt caaggtacat tctttctcac t 1001
<210> 15
<211> 1001
<212> DNA
<213> Brassica napus
<400> 15
gatctttgtg gaaggtggaa ggcgacctca ctgctgagga ggaagctaag gcgttggcga 60
tgggcgtggc gaaattagga cattaagtcc attgatgctc tacaaaatct tatgtgatta 120
ctgaagtctg aagaagtttg tccaagtgtc gtttgtttga agtcaaaaat aaagatgtag 180
caggattatc aagttctgat cattaaaagt cctattataa tttctatgtt tcatcatcac 240
tttgaagttc agttaatcaa aagtacgatt caagaatatt ccagtactgt ttctcgatcc 300
attattacca aaaagtttag ctaattatct tcctggaaac ttcttctgtt ccccccatag 360
agaaagttgt cctgccttta gttccagatt aaataagatg agtatcaagt acccatatgt 420
attttcttcc aaaatataag aacataatat ccaactataa tttaagaaaa aacaaagatt 480
agtggagaac gttaaaaaat rctcttatat aaaagtttaa tatattttat gaatatttaa 540
attttagttt tttttaaaaa aaaagtctca aaatcaatga cagagagggt gacattaaat 600
taattaatct ttctttattt ggcctgagat gcatgctgct tataatagtt agttgcttcc 660
agaggaaaca catattcaaa cagacaagat tagctacgac agttgcctgg taatattttt 720
tattttatta ggcttcgttt ggaatgatta ttataatttg gtatatgata gagagcttgg 780
gctgtgttct cacattattc aaggtacatt ctttctcact ataattttct ttttacgtta 840
aattcaactc aaaaccaatt gctcaagtaa tactaatttc accattaatt ttgcaatatt 900
ttggtagcaa tcgacgagac caattttggg acgaatcggt ttattgatcg tattgcatgg 960
acaccatatt attttaggta aactttcaac gcaaaaccaa t 1001
<210> 16
<211> 1001
<212> DNA
<213> Brassica napus
<400> 16
tgatcattaa aagtcctatt ataatttcta tgtttcatca tcactttgaa gttcagttaa 60
tcaaaagtac gattcaagaa tattccagta ctgtttctcg atccattatt accaaaaagt 120
ttagctaatt atcttcctgg aaacttcttc tgttcccccc atagagaaag ttgtcctgcc 180
tttagttcca gattaaataa gatgagtatc aagtacccat atgtattttc ttccaaaata 240
taagaacata atatccaact ataatttaag aaaaaacaaa gattagtgga gaacgttaaa 300
aaatactctt atataaaagt ttaatatatt ttatgaatat ttaaatttta gtttttttta 360
aaaaaaaagt ctcaaaatca atgacagaga gggtgacatt aaattaatta atctttcttt 420
atttggcctg agatgcatgc tgcttataat agttagttgc ttccagagga aacacatatt 480
caaacagaca agattagcta ygacagttgc ctggtaatat tttttatttt attaggcttc 540
gtttggaatg attattataa tttggtatat gatagagagc ttgggctgtg ttctcacatt 600
attcaaggta cattctttct cactataatt ttctttttac gttaaattca actcaaaacc 660
aattgctcaa gtaatactaa tttcaccatt aattttgcaa tattttggta gcaatcgacg 720
agaccaattt tgggacgaat cggtttattg atcgtattgc atggacacca tattatttta 780
ggtaaacttt caacgcaaaa ccaatagacc ttaatgataa atcgtttcaa acttttgatt 840
taacttttca tttcagtgca aacgttatcg cttttagatt ccttgttaaa ctttcaggca 900
aataaactgg aagcagactc aaaacgataa ggaaagagct cgaagaacca actgaagtgg 960
aaagtgaact gaaacgaaga cttgaccagc taactaatca t 1001
<210> 17
<211> 121
<212> DNA
<213> Brassica napus
<400> 17
tgcttataat agttagttgc ttccagaggr aacacatatt caaacagaca agattagcta 60
ygacagttgc ctggtaatat tttttatttt attaggcttc gtttggaatg attattataa 120
t 121
<210> 18
<211> 1001
<212> DNA
<213> Brassica napus
<400> 18
gtattgcatg gacaccatat tattttaggt aaactttcaa cgcaaaacca atagacctta 60
atgataaatc gtttcaaact tttgatttaa cttttcattt cagtgcaaac gttatcgctt 120
ttagattcct tgttaaactt tcaggcaaat aaactggaag cagactcaaa acgataagga 180
aagagctcga agaaccaact gaagtggaaa gtgaactgaa acgaagactt gaccagctaa 240
ctaatcatct tactcaaaaa caatcccagg tatatgaata cttttaaata aaagagaatt 300
cagacaaaac taatataaaa acttccaact ctcctgccaa atttaccaag tcgactttcc 360
tcataggaag caactctatc gtttagaatc catattttaa ctacttcaca ctattctctc 420
cataatctct ctctcataca aatacattga aggttttgac aacaaacaca aagctctaga 480
actcaagcag gagcaattga sactctttca atctcaacta catactccag cgaagcagaa 540
ggcggtatct gaaaacccga ttcaagttca gcaccctctt ctccaaatcc taaagctggt 600
ggaacgatca ctctcctttt acctccggcc ttcatcgacc tcagaacata atctacgcct 660
tcgcataatc ctttgctata tggctttgaa cccaccacaa gtgccaatgg cttcttattc 720
ttgtctttgc ttccaaatgt gtcaacaaac acttgtcccg tttcttgcac ttgtcccttc 780
atattaatca ctaccaaatc acctgctctt ggtgttgccc ctcctccaag ccgtagatca 840
tagtacctgc atagtttgat tcaatatagt ccaagacttg accgaagcta aggagaccca 900
ttgacaaaat gtacctaatg ccattgggca agacaatctc cttctcttct tcgacgtctc 960
tacaaacaca ccatacaaaa gcagttccaa agctttgtga g 1001
<210> 19
<211> 1001
<212> DNA
<213> Brassica napus
<400> 19
atcatcttac tcaaaaacaa tcccaggtat atgaatactt ttaaataaaa gagaattcag 60
acaaaactaa tataaaaact tccaactctc ctgccaaatt taccaagtcg actttcctca 120
taggaagcaa ctctatcgtt tagaatccat attttaacta cttcacacta ttctctccat 180
aatctctctc tcatacaaat acattgaagg ttttgacaac aaacacaaag ctctagaact 240
caagcaggag caattgagac tctttcaatc tcaactacat actccagcga agcagaaggc 300
ggtatctgaa aacccgattc aagttcagca ccctcttctc caaatcctaa agctggtgga 360
acgatcactc tccttttacc tccggccttc atcgacctca gaacataatc tacgccttcg 420
cataatcctt tgctatatgg ctttgaaccc accacaagtg ccaatggctt cttattcttg 480
tctttgcttc caaatgtgtc macaaacact tgtcccgttt cttgcacttg tcccttcata 540
ttaatcacta ccaaatcacc tgctcttggt gttgcccctc ctccaagccg tagatcatag 600
tacctgcata gtttgattca atatagtcca agacttgacc gaagctaagg agacccattg 660
acaaaatgta cctaatgcca ttgggcaaga caatctcctt ctcttcttcg acgtctctac 720
aaacacacca tacaaaagca gttccaaagc tttgtgagag tgatgacaac taagacagta 780
acttgaagct atggagatgt gaaaaccttg tgttagcttc ttcttgagaa acctcaagcc 840
gtgttttgat ctgctcggag atcacaccga aagctagaaa ccccgcccag gcaagacccg 900
caccgattcc aaaccgtctg gtcaaagaag aagcgaccca atccgtcgtc tcaacgctgg 960
tcttcttcct cttctgttgc gatgcgaggg gttgctccgt c 1001
<210> 20
<211> 1001
<212> DNA
<213> Brassica napus
<400> 20
atgaatactt ttaaataaaa gagaattcag acaaaactaa tataaaaact tccaactctc 60
ctgccaaatt taccaagtcg actttcctca taggaagcaa ctctatcgtt tagaatccat 120
attttaacta cttcacacta ttctctccat aatctctctc tcatacaaat acattgaagg 180
ttttgacaac aaacacaaag ctctagaact caagcaggag caattgagac tctttcaatc 240
tcaactacat actccagcga agcagaaggc ggtatctgaa aacccgattc aagttcagca 300
ccctcttctc caaatcctaa agctggtgga acgatcactc tccttttacc tccggccttc 360
atcgacctca gaacataatc tacgccttcg cataatcctt tgctatatgg ctttgaaccc 420
accacaagtg ccaatggctt cttattcttg tctttgcttc caaatgtgtc aacaaacact 480
tgtcccgttt cttgcacttg ycccttcata ttaatcacta ccaaatcacc tgctcttggt 540
gttgcccctc ctccaagccg tagatcatag tacctgcata gtttgattca atatagtcca 600
agacttgacc gaagctaagg agacccattg acaaaatgta cctaatgcca ttgggcaaga 660
caatctcctt ctcttcttcg acgtctctac aaacacacca tacaaaagca gttccaaagc 720
tttgtgagag tgatgacaac taagacagta acttgaagct atggagatgt gaaaaccttg 780
tgttagcttc ttcttgagaa acctcaagcc gtgttttgat ctgctcggag atcacaccga 840
aagctagaaa ccccgcccag gcaagacccg caccgattcc aaaccgtctg gtcaaagaag 900
aagcgaccca atccgtcgtc tcaacgctgg tcttcttcct cttctgttgc gatgcgaggg 960
gttgctccgt ctttacggat tgagtcggag tagaagaaga c 1001
<210> 21
<211> 1001
<212> DNA
<213> Brassica napus
<400> 21
tcaaagaaga agcgacccaa tccgtcgtct caacgctggt cttcttcctc ttctgttgcg 60
atgcgagggg ttgctccgtc tttacggatt gagtcggagt agaagaagac tctggctgag 120
aaggagggtt ctgctccgga ggagtggaag aagaggcgca acagaggtga tacggcgccg 180
tcttcgtgaa cggctttgaa aggaacggag ctgtaacggt gaatagattc gccatttcgg 240
cataaaataa aataaaaacc tcagctttat tataagtata taaacgctta tcctgttcgt 300
gtgattcatt ttaaagacag aagtcaagcc aagttcttgt cactgtcagt gataaaccga 360
atccggttag gctaaaccgg gtcgtcgaaa ttattaaaaa aaattaaatt gtttcttctt 420
cttcttctcc tttctctctc caatcagtta ggaagaaggt cgtgacccac tccgaaggac 480
aaaaccgaga gacgatccga kaaataaggt gaatttgacg agaatcatta ggctgagaag 540
gaaactcgga gacccaaaat cgtaaatcac caatctttaa tctgtttttc taattcagta 600
gtagtagttg atgggtggtg gtgggaatct cgtcgacggt gttcgtcgtt ggctttttca 660
acgaccctct tcttccaata ataatcctca cgaacccatt gttccaaagt ctgatacttt 720
ttctattccc catcatcaat ctgagcttat cattaccgaa gatctcgatt tctctggtct 780
caagcttatc aaagttccca aacgtcatca cttacccatg gatcctcaaa agaaggtacc 840
ttttggcgcg atcactgatt gtgtagacat catttgatct gtgatctttg tttgattgaa 900
gtttacttct attaatgttt tgtacattgt tcaacaagta gctagatttt gattaggcct 960
tttatagggt gttattgatt attgatttat ttatttattt g 1001
<210> 22
<211> 1001
<212> DNA
<213> Brassica napus
<400> 22
attaggctga gaaggaaact cggagaccca aaatcgtaaa tcaccaatct ttaatctgtt 60
tttctaattc agtagtagta gttgatgggt ggtggtggga atctcgtcga cggtgttcgt 120
cgttggcttt ttcaacgacc ctcttcttcc aataataatc ctcacgaacc cattgttcca 180
aagtctgata ctttttctat tccccatcat caatctgagc ttatcattac cgaagatctc 240
gatttctctg gtctcaagct tatcaaagtt cccaaacgtc atcacttacc catggatcct 300
caaaagaagg taccttttgg cgcgatcact gattgtgtag acatcatttg atctgtgatc 360
tttgtttgat tgaagtttac ttctattaat gttttgtaca ttgttcaaca agtagctaga 420
ttttgattag gccttttata gggtgttatt gattattgat ttatttattt atttgattgg 480
atcctactgt ttgttcaggg ygtgcaggaa aaggacttct tcacggagta cggagaagca 540
aacaggtacc aggttcaaga agtcgttggt aaaggaagct acggtgttgt ggcctctgct 600
ctagacacac acactggcga aagagttgct atcaagaaga tcaacgacgt ctttgagcat 660
gtctctgatg caaccaggat tctcagggag atcaagctgc tgaggttgct taagcatccg 720
gatgttgtgg agattaagca tattatgctg cctccttctc gtagagagtt cagggatatt 780
tacgttgtgt ttgagctgat ggagtctgat cttcatcagg tgattaaggc gaatgatgat 840
ttgactcctg atcattatca gttcttcttg tatcagcttc tccgtggtct caaatatgtc 900
cacgcaggtt aagtttctgg ttttaaaaca gtcttctctt ttgtctgtct ttattgaaac 960
gtttgtgtgt tttcagctaa tgtgtttcat cgggatttga a 1001
<210> 23
<211> 1001
<212> DNA
<213> Brassica napus
<400> 23
gacccaaaat cgtaaatcac caatctttaa tctgtttttc taattcagta gtagtagttg 60
atgggtggtg gtgggaatct cgtcgacggt gttcgtcgtt ggctttttca acgaccctct 120
tcttccaata ataatcctca cgaacccatt gttccaaagt ctgatacttt ttctattccc 180
catcatcaat ctgagcttat cattaccgaa gatctcgatt tctctggtct caagcttatc 240
aaagttccca aacgtcatca cttacccatg gatcctcaaa agaaggtacc ttttggcgcg 300
atcactgatt gtgtagacat catttgatct gtgatctttg tttgattgaa gtttacttct 360
attaatgttt tgtacattgt tcaacaagta gctagatttt gattaggcct tttatagggt 420
gttattgatt attgatttat ttatttattt gattggatcc tactgtttgt tcagggtgtg 480
caggaaaagg acttcttcac rgagtacgga gaagcaaaca ggtaccaggt tcaagaagtc 540
gttggtaaag gaagctacgg tgttgtggcc tctgctctag acacacacac tggcgaaaga 600
gttgctatca agaagatcaa cgacgtcttt gagcatgtct ctgatgcaac caggattctc 660
agggagatca agctgctgag gttgcttaag catccggatg ttgtggagat taagcatatt 720
atgctgcctc cttctcgtag agagttcagg gatatttacg ttgtgtttga gctgatggag 780
tctgatcttc atcaggtgat taaggcgaat gatgatttga ctcctgatca ttatcagttc 840
ttcttgtatc agcttctccg tggtctcaaa tatgtccacg caggttaagt ttctggtttt 900
aaaacagtct tctcttttgt ctgtctttat tgaaacgttt gtgtgttttc agctaatgtg 960
tttcatcggg atttgaaacc aaagaacatt ctagctaatg c 1001
<210> 24
<211> 1001
<212> DNA
<213> Brassica napus
<400> 24
tttcaacgac cctcttcttc caataataat cctcacgaac ccattgttcc aaagtctgat 60
actttttcta ttccccatca tcaatctgag cttatcatta ccgaagatct cgatttctct 120
ggtctcaagc ttatcaaagt tcccaaacgt catcacttac ccatggatcc tcaaaagaag 180
gtaccttttg gcgcgatcac tgattgtgta gacatcattt gatctgtgat ctttgtttga 240
ttgaagttta cttctattaa tgttttgtac attgttcaac aagtagctag attttgatta 300
ggccttttat agggtgttat tgattattga tttatttatt tatttgattg gatcctactg 360
tttgttcagg gtgtgcagga aaaggacttc ttcacggagt acggagaagc aaacaggtac 420
caggttcaag aagtcgttgg taaaggaagc tacggtgttg tggcctctgc tctagacaca 480
cacactggcg aaagagttgc yatcaagaag atcaacgacg tctttgagca tgtctctgat 540
gcaaccagga ttctcaggga gatcaagctg ctgaggttgc ttaagcatcc ggatgttgtg 600
gagattaagc atattatgct gcctccttct cgtagagagt tcagggatat ttacgttgtg 660
tttgagctga tggagtctga tcttcatcag gtgattaagg cgaatgatga tttgactcct 720
gatcattatc agttcttctt gtatcagctt ctccgtggtc tcaaatatgt ccacgcaggt 780
taagtttctg gttttaaaac agtcttctct tttgtctgtc tttattgaaa cgtttgtgtg 840
ttttcagcta atgtgtttca tcgggatttg aaaccaaaga acattctagc taatgctgat 900
tgcaagttga agatctgtga ttttggactc gctcgtgtct cttttaacga cgcaccaact 960
gctatattct ggactgtgag tcctctaatt tgaatgcagc a 1001
<210> 25
<211> 1001
<212> DNA
<213> Brassica napus
<400> 25
cccattgttc caaagtctga tactttttct attccccatc atcaatctga gcttatcatt 60
accgaagatc tcgatttctc tggtctcaag cttatcaaag ttcccaaacg tcatcactta 120
cccatggatc ctcaaaagaa ggtacctttt ggcgcgatca ctgattgtgt agacatcatt 180
tgatctgtga tctttgtttg attgaagttt acttctatta atgttttgta cattgttcaa 240
caagtagcta gattttgatt aggcctttta tagggtgtta ttgattattg atttatttat 300
ttatttgatt ggatcctact gtttgttcag ggtgtgcagg aaaaggactt cttcacggag 360
tacggagaag caaacaggta ccaggttcaa gaagtcgttg gtaaaggaag ctacggtgtt 420
gtggcctctg ctctagacac acacactggc gaaagagttg ctatcaagaa gatcaacgac 480
gtctttgagc atgtctctga ygcaaccagg attctcaggg agatcaagct gctgaggttg 540
cttaagcatc cggatgttgt ggagattaag catattatgc tgcctccttc tcgtagagag 600
ttcagggata tttacgttgt gtttgagctg atggagtctg atcttcatca ggtgattaag 660
gcgaatgatg atttgactcc tgatcattat cagttcttct tgtatcagct tctccgtggt 720
ctcaaatatg tccacgcagg ttaagtttct ggttttaaaa cagtcttctc ttttgtctgt 780
ctttattgaa acgtttgtgt gttttcagct aatgtgtttc atcgggattt gaaaccaaag 840
aacattctag ctaatgctga ttgcaagttg aagatctgtg attttggact cgctcgtgtc 900
tcttttaacg acgcaccaac tgctatattc tggactgtga gtcctctaat ttgaatgcag 960
cagagcttct cattaaactg tttgtgaact cactctttta t 1001
<210> 26
<211> 1001
<212> DNA
<213> Brassica napus
<400> 26
ttacccatgg atcctcaaaa gaaggtacct tttggcgcga tcactgattg tgtagacatc 60
atttgatctg tgatctttgt ttgattgaag tttacttcta ttaatgtttt gtacattgtt 120
caacaagtag ctagattttg attaggcctt ttatagggtg ttattgatta ttgatttatt 180
tatttatttg attggatcct actgtttgtt cagggtgtgc aggaaaagga cttcttcacg 240
gagtacggag aagcaaacag gtaccaggtt caagaagtcg ttggtaaagg aagctacggt 300
gttgtggcct ctgctctaga cacacacact ggcgaaagag ttgctatcaa gaagatcaac 360
gacgtctttg agcatgtctc tgatgcaacc aggattctca gggagatcaa gctgctgagg 420
ttgcttaagc atccggatgt tgtggagatt aagcatatta tgctgcctcc ttctcgtaga 480
gagttcaggg atatttacgt ygtgtttgag ctgatggagt ctgatcttca tcaggtgatt 540
aaggcgaatg atgatttgac tcctgatcat tatcagttct tcttgtatca gcttctccgt 600
ggtctcaaat atgtccacgc aggttaagtt tctggtttta aaacagtctt ctcttttgtc 660
tgtctttatt gaaacgtttg tgtgttttca gctaatgtgt ttcatcggga tttgaaacca 720
aagaacattc tagctaatgc tgattgcaag ttgaagatct gtgattttgg actcgctcgt 780
gtctctttta acgacgcacc aactgctata ttctggactg tgagtcctct aatttgaatg 840
cagcagagct tctcattaaa ctgtttgtga actcactctt ttatctatgt tttgtaggat 900
tatgtagcta ctcggtggta ccgtgcccct gaactctgtg gatcgttttt ctccaaagta 960
agattctttt tttttgttta ttcactgaac ctctctgtat c 1001
<210> 27
<211> 1001
<212> DNA
<213> Brassica napus
<400> 27
aaaacagtct tctcttttgt ctgtctttat tgaaacgttt gtgtgttttc agctaatgtg 60
tttcatcggg atttgaaacc aaagaacatt ctagctaatg ctgattgcaa gttgaagatc 120
tgtgattttg gactcgctcg tgtctctttt aacgacgcac caactgctat attctggact 180
gtgagtcctc taatttgaat gcagcagagc ttctcattaa actgtttgtg aactcactct 240
tttatctatg ttttgtagga ttatgtagct actcggtggt accgtgcccc tgaactctgt 300
ggatcgtttt tctccaaagt aagattcttt ttttttgttt attcactgaa cctctctgta 360
tcacaagaac ggacttcttg atctaggtcc tatattttca taagatatac cgtctaatgc 420
taagttaact ttcagtacac tcctgcgatt gatatatgga gtgttggttg catttttgcg 480
gaaatgatat tgggaaagcc wttgtttccc gggaagaacg tggtgcacca acttgatctt 540
atgactgact ttcttggcac tcctccgcct gagtccatat caagggttag tcactcaaac 600
atgtgttaca ttcccatcat ttgagagcta gttaatgagt tttttttgtt tttttttgca 660
atcttgaaat tatgacagat aagaaatgaa aaggcgagga gatatctaag cagcatgagg 720
aagaaacagc cggttccttt ctctcacaag ttccctaaag ctgatccttt ggctctccgc 780
cttctcgaac gccttattgc ctttgatcct aaagatcgtg tctcagctga agatgtaagc 840
gacaagcaac tttcattttt tttttaatta caaagactta aaactctcaa gttcattatt 900
ctgatttggt tatttacagg cactagctga tccttatttc agtggtctgt caaactcaga 960
gcgtgaacca tcaacgcagc caatctcaaa gcttgagttt g 1001
<210> 28
<211> 1002
<212> DNA
<213> Brassica napus
<220>
<221> misc_feature
<222> (502)..(502)
<223> 其中n为插入/缺失 [+/TT]
<400> 28
ttttggactc gctcgtgtct cttttaacga cgcaccaact gctatattct ggactgtgag 60
tcctctaatt tgaatgcagc agagcttctc attaaactgt ttgtgaactc actcttttat 120
ctatgttttg taggattatg tagctactcg gtggtaccgt gcccctgaac tctgtggatc 180
gtttttctcc aaagtaagat tctttttttt tgtttattca ctgaacctct ctgtatcaca 240
agaacggact tcttgatcta ggtcctatat tttcataaga tataccgtct aatgctaagt 300
taactttcag tacactcctg cgattgatat atggagtgtt ggttgcattt ttgcggaaat 360
gatattggga aagcctttgt ttcccgggaa gaacgtggtg caccaacttg atcttatgac 420
tgactttctt ggcactcctc cgcctgagtc catatcaagg gttagtcact caaacatgtg 480
ttacattccc atcatttgag angctagtta atgagttttt tttgtttttt tttgcaatct 540
tgaaattatg acagataaga aatgaaaagg cgaggagata tctaagcagc atgaggaaga 600
aacagccggt tcctttctct cacaagttcc ctaaagctga tcctttggct ctccgccttc 660
tcgaacgcct tattgccttt gatcctaaag atcgtgtctc agctgaagat gtaagcgaca 720
agcaactttc attttttttt taattacaaa gacttaaaac tctcaagttc attattctga 780
tttggttatt tacaggcact agctgatcct tatttcagtg gtctgtcaaa ctcagagcgt 840
gaaccatcaa cgcagccaat ctcaaagctt gagtttgatt ttgagagaaa gaagttgaac 900
aaagatgacg tcagagaatt aatctaccga gaggtaacac aaaaaaaaat gcttttgact 960
atgtcttatt gttctcttca ttgatctaac attcacttta tc 1002
<210> 29
<211> 1001
<212> DNA
<213> Brassica napus
<400> 29
ttggactcgc tcgtgtctct tttaacgacg caccaactgc tatattctgg actgtgagtc 60
ctctaatttg aatgcagcag agcttctcat taaactgttt gtgaactcac tcttttatct 120
atgttttgta ggattatgta gctactcggt ggtaccgtgc ccctgaactc tgtggatcgt 180
ttttctccaa agtaagattc tttttttttg tttattcact gaacctctct gtatcacaag 240
aacggacttc ttgatctagg tcctatattt tcataagata taccgtctaa tgctaagtta 300
actttcagta cactcctgcg attgatatat ggagtgttgg ttgcattttt gcggaaatga 360
tattgggaaa gcctttgttt cccgggaaga acgtggtgca ccaacttgat cttatgactg 420
actttcttgg cactcctccg cctgagtcca tatcaagggt tagtcactca aacatgtgtt 480
acattcccat catttgagag ytagttaatg agtttttttt gttttttttt gcaatcttga 540
aattatgaca gataagaaat gaaaaggcga ggagatatct aagcagcatg aggaagaaac 600
agccggttcc tttctctcac aagttcccta aagctgatcc tttggctctc cgccttctcg 660
aacgccttat tgcctttgat cctaaagatc gtgtctcagc tgaagatgta agcgacaagc 720
aactttcatt ttttttttaa ttacaaagac ttaaaactct caagttcatt attctgattt 780
ggttatttac aggcactagc tgatccttat ttcagtggtc tgtcaaactc agagcgtgaa 840
ccatcaacgc agccaatctc aaagcttgag tttgattttg agagaaagaa gttgaacaaa 900
gatgacgtca gagaattaat ctaccgagag gtaacacaaa aaaaaatgct tttgactatg 960
tcttattgtt ctcttcattg atctaacatt cactttatct t 1001
<210> 30
<211> 1001
<212> DNA
<213> Brassica napus
<400> 30
agtacactcc tgcgattgat atatggagtg ttggttgcat ttttgcggaa atgatattgg 60
gaaagccttt gtttcccggg aagaacgtgg tgcaccaact tgatcttatg actgactttc 120
ttggcactcc tccgcctgag tccatatcaa gggttagtca ctcaaacatg tgttacattc 180
ccatcatttg agagctagtt aatgagtttt ttttgttttt ttttgcaatc ttgaaattat 240
gacagataag aaatgaaaag gcgaggagat atctaagcag catgaggaag aaacagccgg 300
ttcctttctc tcacaagttc cctaaagctg atcctttggc tctccgcctt ctcgaacgcc 360
ttattgcctt tgatcctaaa gatcgtgtct cagctgaaga tgtaagcgac aagcaacttt 420
catttttttt ttaattacaa agacttaaaa ctctcaagtt cattattctg atttggttat 480
ttacaggcac tagctgatcc wtatttcagt ggtctgtcaa actcagagcg tgaaccatca 540
acgcagccaa tctcaaagct tgagtttgat tttgagagaa agaagttgaa caaagatgac 600
gtcagagaat taatctaccg agaggtaaca caaaaaaaaa tgcttttgac tatgtcttat 660
tgttctcttc attgatctaa cattcacttt atctttggga aaaacattta gatattggag 720
tatcatcctc agatgctgga ggagtacaag cgcggtggtg atcagctcag cttcatgtac 780
cctaggttag ctaattaaac acctcatgaa ctataattcc ctgaaaacag aatgaaacca 840
agaactcttc tgttgtttac gcagtggggt tgatcggttc aagaggcagt ttgctcacct 900
tgaagagaat caaggtaaac caggagcagg ggcaggagga ggaagaagta ctgcaatgca 960
tagacaccat gcttccttgc caatgtaatg tctttttcac a 1001
<210> 31
<211> 1001
<212> DNA
<213> Brassica napus
<400> 31
acactcctgc gattgatata tggagtgttg gttgcatttt tgcggaaatg atattgggaa 60
agcctttgtt tcccgggaag aacgtggtgc accaacttga tcttatgact gactttcttg 120
gcactcctcc gcctgagtcc atatcaaggg ttagtcactc aaacatgtgt tacattccca 180
tcatttgaga gctagttaat gagttttttt tgtttttttt tgcaatcttg aaattatgac 240
agataagaaa tgaaaaggcg aggagatatc taagcagcat gaggaagaaa cagccggttc 300
ctttctctca caagttccct aaagctgatc ctttggctct ccgccttctc gaacgcctta 360
ttgcctttga tcctaaagat cgtgtctcag ctgaagatgt aagcgacaag caactttcat 420
ttttttttta attacaaaga cttaaaactc tcaagttcat tattctgatt tggttattta 480
caggcactag ctgatcctta yttcagtggt ctgtcaaact cagagcgtga accatcaacg 540
cagccaatct caaagcttga gtttgatttt gagagaaaga agttgaacaa agatgacgtc 600
agagaattaa tctaccgaga ggtaacacaa aaaaaaatgc ttttgactat gtcttattgt 660
tctcttcatt gatctaacat tcactttatc tttgggaaaa acatttagat attggagtat 720
catcctcaga tgctggagga gtacaagcgc ggtggtgatc agctcagctt catgtaccct 780
aggttagcta attaaacacc tcatgaacta taattccctg aaaacagaat gaaaccaaga 840
actcttctgt tgtttacgca gtggggttga tcggttcaag aggcagtttg ctcaccttga 900
agagaatcaa ggtaaaccag gagcaggggc aggaggagga agaagtactg caatgcatag 960
acaccatgct tccttgccaa tgtaatgtct ttttcacaga a 1001
<210> 32
<211> 1002
<212> DNA
<213> Brassica napus
<220>
<221> misc_feature
<222> (502)..(502)
<223> 其中n为插入/缺失 [+/A]
<400> 32
gtccatatca agggttagtc actcaaacat gtgttacatt cccatcattt gagagctagt 60
taatgagttt tttttgtttt tttttgcaat cttgaaatta tgacagataa gaaatgaaaa 120
ggcgaggaga tatctaagca gcatgaggaa gaaacagccg gttcctttct ctcacaagtt 180
ccctaaagct gatcctttgg ctctccgcct tctcgaacgc cttattgcct ttgatcctaa 240
agatcgtgtc tcagctgaag atgtaagcga caagcaactt tcattttttt tttaattaca 300
aagacttaaa actctcaagt tcattattct gatttggtta tttacaggca ctagctgatc 360
cttatttcag tggtctgtca aactcagagc gtgaaccatc aacgcagcca atctcaaagc 420
ttgagtttga ttttgagaga aagaagttga acaaagatga cgtcagagaa ttaatctacc 480
gagaggtaac acaaaaaaaa antgcttttg actatgtctt attgttctct tcattgatct 540
aacattcact ttatctttgg gaaaaacatt tagatattgg agtatcatcc tcagatgctg 600
gaggagtaca agcgcggtgg tgatcagctc agcttcatgt accctaggtt agctaattaa 660
acacctcatg aactataatt ccctgaaaac agaatgaaac caagaactct tctgttgttt 720
acgcagtggg gttgatcggt tcaagaggca gtttgctcac cttgaagaga atcaaggtaa 780
accaggagca ggggcaggag gaggaagaag tactgcaatg catagacacc atgcttcctt 840
gccaatgtaa tgtctttttc acagaatctc ttgctttgct ctctctttct ctgaaagcgt 900
tgggcttctt tgtgattgtg tgttgcagag agagagttcc tgctcagagt ggtcagactg 960
tagaagaaag cagtgatgtt gagagaagag cagcagctgc tg 1002
<210> 33
<211> 1001
<212> DNA
<213> Brassica napus
<400> 33
ctagctgatc cttatttcag tggtctgtca aactcagagc gtgaaccatc aacgcagcca 60
atctcaaagc ttgagtttga ttttgagaga aagaagttga acaaagatga cgtcagagaa 120
ttaatctacc gagaggtaac acaaaaaaaa atgcttttga ctatgtctta ttgttctctt 180
cattgatcta acattcactt tatctttggg aaaaacattt agatattgga gtatcatcct 240
cagatgctgg aggagtacaa gcgcggtggt gatcagctca gcttcatgta ccctaggtta 300
gctaattaaa cacctcatga actataattc cctgaaaaca gaatgaaacc aagaactctt 360
ctgttgttta cgcagtgggg ttgatcggtt caagaggcag tttgctcacc ttgaagagaa 420
tcaaggtaaa ccaggagcag gggcaggagg aggaagaagt actgcaatgc atagacacca 480
tgcttccttg ccaatgtaat rtctttttca cagaatctct tgctttgctc tctctttctc 540
tgaaagcgtt gggcttcttt gtgattgtgt gttgcagaga gagagttcct gctcagagtg 600
gtcagactgt agaagaaagc agtgatgttg agagaagagc agcagctgct gtggcttcaa 660
ctttggaatc tgaggaagca gacaatggag gaggttacag tgctcgtagc ctcatgaaga 720
gttcgagcat cagtggttct aaatgcatcg gtgtccaatc taaaaccgac aaagaggtta 780
gttagttagt tagttagtgg agttaaaaaa acagaggatc ttgaaaggaa catggagatg 840
gagtttgctt acttactgtt gtttctgttc tgtgttgtag gacaccatag ctgaggaagg 900
agatgatgaa tcagtggcgg agcttactga tagagttgct tctcttcgta attcttaaaa 960
cgtttttgtt tttttttttg gcgtttggtg aaagctttct g 1001
<210> 34
<211> 1001
<212> DNA
<213> Brassica napus
<220>
<221> misc_feature
<222> (501)..(501)
<223> 其中n为插入/缺失 [-/T]
<400> 34
atccttattt cagtggtctg tcaaactcag agcgtgaacc atcaacgcag ccaatctcaa 60
agcttgagtt tgattttgag agaaagaagt tgaacaaaga tgacgtcaga gaattaatct 120
accgagaggt aacacaaaaa aaaatgcttt tgactatgtc ttattgttct cttcattgat 180
ctaacattca ctttatcttt gggaaaaaca tttagatatt ggagtatcat cctcagatgc 240
tggaggagta caagcgcggt ggtgatcagc tcagcttcat gtaccctagg ttagctaatt 300
aaacacctca tgaactataa ttccctgaaa acagaatgaa accaagaact cttctgttgt 360
ttacgcagtg gggttgatcg gttcaagagg cagtttgctc accttgaaga gaatcaaggt 420
aaaccaggag caggggcagg aggaggaaga agtactgcaa tgcatagaca ccatgcttcc 480
ttgccaatgt aatgtctttt ncacagaatc tcttgctttg ctctctcttt ctctgaaagc 540
gttgggcttc tttgtgattg tgtgttgcag agagagagtt cctgctcaga gtggtcagac 600
tgtagaagaa agcagtgatg ttgagagaag agcagcagct gctgtggctt caactttgga 660
atctgaggaa gcagacaatg gaggaggtta cagtgctcgt agcctcatga agagttcgag 720
catcagtggt tctaaatgca tcggtgtcca atctaaaacc gacaaagagg ttagttagtt 780
agttagttag tggagttaaa aaaacagagg atcttgaaag gaacatggag atggagtttg 840
cttacttact gttgtttctg ttctgtgttg taggacacca tagctgagga aggagatgat 900
gaatcagtgg cggagcttac tgatagagtt gcttctcttc gtaattctta aaacgttttt 960
gttttttttt ttggcgtttg gtgaaagctt tctggtgaaa a 1001
<210> 35
<211> 1001
<212> DNA
<213> Brassica napus
<400> 35
acgtcagaga attaatctac cgagaggtaa cacaaaaaaa aatgcttttg actatgtctt 60
attgttctct tcattgatct aacattcact ttatctttgg gaaaaacatt tagatattgg 120
agtatcatcc tcagatgctg gaggagtaca agcgcggtgg tgatcagctc agcttcatgt 180
accctaggtt agctaattaa acacctcatg aactataatt ccctgaaaac agaatgaaac 240
caagaactct tctgttgttt acgcagtggg gttgatcggt tcaagaggca gtttgctcac 300
cttgaagaga atcaaggtaa accaggagca ggggcaggag gaggaagaag tactgcaatg 360
catagacacc atgcttcctt gccaatgtaa tgtctttttc acagaatctc ttgctttgct 420
ctctctttct ctgaaagcgt tgggcttctt tgtgattgtg tgttgcagag agagagttcc 480
tgctcagagt ggtcagactg yagaagaaag cagtgatgtt gagagaagag cagcagctgc 540
tgtggcttca actttggaat ctgaggaagc agacaatgga ggaggttaca gtgctcgtag 600
cctcatgaag agttcgagca tcagtggttc taaatgcatc ggtgtccaat ctaaaaccga 660
caaagaggtt agttagttag ttagttagtg gagttaaaaa aacagaggat cttgaaagga 720
acatggagat ggagtttgct tacttactgt tgtttctgtt ctgtgttgta ggacaccata 780
gctgaggaag gagatgatga atcagtggcg gagcttactg atagagttgc ttctcttcgt 840
aattcttaaa acgtttttgt tttttttttt ggcgtttggt gaaagctttc tggtgaaaat 900
tggtttctac attttatttt cacttcttcc acatctatct tcgtggttgg gtttgatttg 960
ttggatttaa tagtttgggg gcgagaatga gaccttttta a 1001
<210> 36
<211> 1001
<212> DNA
<213> Brassica napus
<400> 36
acgcagtggg gttgatcggt tcaagaggca gtttgctcac cttgaagaga atcaaggtaa 60
accaggagca ggggcaggag gaggaagaag tactgcaatg catagacacc atgcttcctt 120
gccaatgtaa tgtctttttc acagaatctc ttgctttgct ctctctttct ctgaaagcgt 180
tgggcttctt tgtgattgtg tgttgcagag agagagttcc tgctcagagt ggtcagactg 240
tagaagaaag cagtgatgtt gagagaagag cagcagctgc tgtggcttca actttggaat 300
ctgaggaagc agacaatgga ggaggttaca gtgctcgtag cctcatgaag agttcgagca 360
tcagtggttc taaatgcatc ggtgtccaat ctaaaaccga caaagaggtt agttagttag 420
ttagttagtg gagttaaaaa aacagaggat cttgaaagga acatggagat ggagtttgct 480
tacttactgt tgtttctgtt ytgtgttgta ggacaccata gctgaggaag gagatgatga 540
atcagtggcg gagcttactg atagagttgc ttctcttcgt aattcttaaa acgtttttgt 600
tttttttttt ggcgtttggt gaaagctttc tggtgaaaat tggtttctac attttatttt 660
cacttcttcc acatctatct tcgtggttgg gtttgatttg ttggatttaa tagtttgggg 720
gcgagaatga gaccttttta ataagaacat ctatctccat gtaatttctt ttatcctttt 780
ctataaattg ttctttcaat ctttttaccg attcagtttg cttaagtaca tcatgaaatc 840
agaattaaac taaaaaatag tatactaaaa aaggaaaaca tccaaaaaac ctttatagtt 900
gaagtaaaca tatatatata tatatatata tatatgcagt ttgctttata ttatgatctg 960
aattagttat atatacatac taagtgtttt tcaaaaatag t 1001
<210> 37
<211> 1001
<212> DNA
<213> Brassica napus
<400> 37
agataccacc ttcaccggtt gaaaacaagt tttgcgagaa tcacgttcga gtaaaattta 60
tgcacttcat ttaaatatta caagtgttta tttattcgac tataaatgtt cgtaaagacc 120
acaattcatt tgaagattta ttttattttt cactctcata aaatcctttt gtattcacca 180
gggtcatcaa tatacataca ctcatctgat ttatatagtt gcatggcagt taattgcaat 240
ttagtcctag gtgatttgtt ctattataaa tcaaaccaag agagttgcat gttttccatg 300
acgaagtatc ttctactagt agattactgg cagttggtgt aatagttacc accagtagaa 360
actagtttac caacgagtac aacgaggatc atccacgtgc aaggagtgat cctcgtaact 420
gacggcagga atgatgtaac gtggcaggac gaaccaaagg gatcgtgggt aacagctgag 480
accgtgatac ggcacgtgtc wtgtttgtgg atgaaaagaa gtgtgaaatg gcctatgcat 540
ggtctatagg ttacactaat ctgaccaaaa gcttctttta accttttcct tttgtttctt 600
ctttcgctta taaccaagtg agaaactgta ttgtatttcc ctgaaaacat tagattaagt 660
atgagggatt acatatactt aaggcatctt taaccttagt ttatttatga taaagttagt 720
ttcagagtaa tatagcatta ttagctttga tggtttgtac aatagtgatg aatttggaca 780
tgaccataaa ctacaagaca cgagtggatt ctcataatat ttgcaccact aaggacaaaa 840
taactcatca tgctactttg ttgaaatata ttaccattat tattaatgta ttataaaaat 900
acgaacaagt tattattgaa ttgggtttac agctttcaag atatatttta tataaaaatg 960
aaaataaaaa caagaatttg tttacatata aaaagcaaac a 1001
<210> 38
<211> 1001
<212> DNA
<213> Brassica napus
<400> 38
gatgaaaaga agtgtgaaat ggcctatgca tggtctatag gttacactaa tctgaccaaa 60
agcttctttt aaccttttcc ttttgtttct tctttcgctt ataaccaagt gagaaactgt 120
attgtatttc cctgaaaaca ttagattaag tatgagggat tacatatact taaggcatct 180
ttaaccttag tttatttatg ataaagttag tttcagagta atatagcatt attagctttg 240
atggtttgta caatagtgat gaatttggac atgaccataa actacaagac acgagtggat 300
tctcataata tttgcaccac taaggacaaa ataactcatc atgctacttt gttgaaatat 360
attaccatta ttattaatgt attataaaaa tacgaacaag ttattattga attgggttta 420
cagctttcaa gatatatttt atataaaaat gaaaataaaa acaagaattt gtttacatat 480
aaaaagcaaa cacgcatgta waataaattg gactgcatgt aaatcattgt tcaaattcat 540
tgtatttgtc gatggattaa ttaaatatct ttttgctata aaataaaatt ttatctttta 600
accaaaaaaa ataaaaaaaa taaaaaaaaa taaaatttta tctatataaa ccattacata 660
gatgtccatc ccaatacgga catgcgctga acacaacaaa cgattctttt taagagaatc 720
tctctctctc tattctctcc acttctctct ctgtggatcg atggcagctt cggttgatcc 780
tttggtggtt ggaagagtga tcggagatgt gttggacatg ttcatcccca ccgccaacat 840
gtctgtctac tttggcccca aacacataac taacggctgc gagatcaaac cctctgccgc 900
agtcaaccct ccaaaagtca acatctccgg caactccaat gagctttaca ctctcgtata 960
catattaatc ttctcgcttc tatccatttt ttgtgctagc t 1001
<210> 39
<211> 685
<212> DNA
<213> Brassica napus
<400> 39
tgtggatcga tggcagcttc ggttgatcct ttggtggttg gaagagtgat cggagatgtg 60
ttggacatgt tcatccccac cgccaacatg tctgtctact ttggccccaa acacataact 120
aacggctgcg agatcaaacc ctctgccgca gtcaaccctc caaaagtcaa catctccggc 180
aactccaatg agctttacac tctcgtgatg actgacccgg acgcacctag cccgagtgag 240
ccgaacatga gagaatgggt ccattggatt gtcgtggata tcccgggagg caccaacccc 300
tcaaaaggaa aggagatact gccatatatg gagccgagac caccggtggg gattcaccgt 360
tacatatttg tacttttcag gcagaactca ccggtgggta tgatggtgca gcagccgcct 420
tcgcgagcca acttcagcac ccgaatgttc gctggacatc tcgatcttgg tttgcctgtg 480
gccacagttt acttcaacgc ccagaaagag ccagcttcac gcagacgctg atgcaygtca 540
accaaaataa aagagagagc cttttccggt tttacctaaa aaccggaccg gaaagaaata 600
tggggtttat atatcaaacc atattttgta tcatccggtt ctcgactata tatatgtgta 660
gatgcatata caattataca aatat 685
<210> 40
<211> 1001
<212> DNA
<213> Brassica napus
<400> 40
atgatttatt atgtttcagg attgtcgtgg atatcccggg aggcaccaac ccctcaaaag 60
gtatgaaaat aaaagccaaa actaaatttt cgatttttga attttaattg tttcgctatt 120
ttccggaatc tcttaattat tatttttcta aacttttttt acaaatgaat ttcacttttt 180
aactcacttt ctaactcact tgcttagata taaaataacg ttagtgtgaa agccactaag 240
aacacatgat aatggttaac tatgtccatg aagacgtgtt tgaatctaat tgaaaaatcc 300
gatacactag tatgttttat tcatttaaac atattatctg tgcaacgtgg tgctttcggt 360
ttgatatgaa atggattccc cgtattgcac gatattgatt ggttcaacaa cacaaatatg 420
catgactctc acatgcatat aggtataaga gtcactatgt aattttcctt ggtttcaagt 480
tatgccccaa aataatacgt ratgtcttct aaaaccaaca tctaatgtat gtctgtacgt 540
gtacactgat gtatatcaac taaacaacgg acacatgtct tcataaaaaa accttaaaca 600
tgacaaagca taagtgaata gaggatgata attattttat ttttattatt agtaccacgg 660
gaaactttga aatcgatata ctagcatgtt tttcatttta ggaaaggaga tactgccata 720
tatggagccg agaccaccgg tggggattca ccgttacata tttgtacttt tcaggcagaa 780
ctcaccggtg ggtatgatgg tgcagcagcc gccttcgcga gccaacttca gcacccgaat 840
gttcgctgga catctcgatc ttggtttgcc tgtggccaca gtttacttca acgcccagaa 900
agagccagct tcacgcagac gctgatgcac gcaaccaaaa taaaagagag agccttttcc 960
ggttttacct aaaaaccgga ccggaaagaa atatggggtt t 1001
<210> 41
<211> 1001
<212> DNA
<213> Brassica napus
<400> 41
tgatttatta tgtttcagga ttgtcgtgga tatcccggga ggcaccaacc cctcaaaagg 60
tatgaaaata aaagccaaaa ctaaattttc gatttttgaa ttttaattgt ttcgctattt 120
tccggaatct cttaattatt atttttctaa acttttttta caaatgaatt tcacttttta 180
actcactttc taactcactt gcttagatat aaaataacgt tagtgtgaaa gccactaaga 240
acacatgata atggttaact atgtccatga agacgtgttt gaatctaatt gaaaaatccg 300
atacactagt atgttttatt catttaaaca tattatctgt gcaacgtggt gctttcggtt 360
tgatatgaaa tggattcccc gtattgcacg atattgattg gttcaacaac acaaatatgc 420
atgactctca catgcatata ggtataagag tcactatgta attttccttg gtttcaagtt 480
atgccccaaa ataatacgta rtgtcttcta aaaccaacat ctaatgtatg tctgtacgtg 540
tacactgatg tatatcaact aaacaacgga cacatgtctt cataaaaaaa ccttaaacat 600
gacaaagcat aagtgaatag aggatgataa ttattttatt tttattatta gtaccacggg 660
aaactttgaa atcgatatac tagcatgttt ttcattttag gaaaggagat actgccatat 720
atggagccga gaccaccggt ggggattcac cgttacatat ttgtactttt caggcagaac 780
tcaccggtgg gtatgatggt gcagcagccg ccttcgcgag ccaacttcag cacccgaatg 840
ttcgctggac atctcgatct tggtttgcct gtggccacag tttacttcaa cgcccagaaa 900
gagccagctt cacgcagacg ctgatgcacg caaccaaaat aaaagagaga gccttttccg 960
gttttaccta aaaaccggac cggaaagaaa tatggggttt a 1001
<210> 42
<211> 1001
<212> DNA
<213> Brassica napus
<400> 42
aggattgtcg tggatatccc gggaggcacc aacccctcaa aaggtatgaa aataaaagcc 60
aaaactaaat tttcgatttt tgaattttaa ttgtttcgct attttccgga atctcttaat 120
tattattttt ctaaactttt tttacaaatg aatttcactt tttaactcac tttctaactc 180
acttgcttag atataaaata acgttagtgt gaaagccact aagaacacat gataatggtt 240
aactatgtcc atgaagacgt gtttgaatct aattgaaaaa tccgatacac tagtatgttt 300
tattcattta aacatattat ctgtgcaacg tggtgctttc ggtttgatat gaaatggatt 360
ccccgtattg cacgatattg attggttcaa caacacaaat atgcatgact ctcacatgca 420
tataggtata agagtcacta tgtaattttc cttggtttca agttatgccc caaaataata 480
cgtaatgtct tctaaaacca rcatctaatg tatgtctgta cgtgtacact gatgtatatc 540
aactaaacaa cggacacatg tcttcataaa aaaaccttaa acatgacaaa gcataagtga 600
atagaggatg ataattattt tatttttatt attagtacca cgggaaactt tgaaatcgat 660
atactagcat gtttttcatt ttaggaaagg agatactgcc atatatggag ccgagaccac 720
cggtggggat tcaccgttac atatttgtac ttttcaggca gaactcaccg gtgggtatga 780
tggtgcagca gccgccttcg cgagccaact tcagcacccg aatgttcgct ggacatctcg 840
atcttggttt gcctgtggcc acagtttact tcaacgccca gaaagagcca gcttcacgca 900
gacgctgatg cacgcaacca aaataaaaga gagagccttt tccggtttta cctaaaaacc 960
ggaccggaaa gaaatatggg gtttatatat caaaccatat t 1001
<210> 43
<211> 1001
<212> DNA
<213> Brassica napus
<400> 43
ttgtttcgct attttccgga atctcttaat tattattttt ctaaactttt tttacaaatg 60
aatttcactt tttaactcac tttctaactc acttgcttag atataaaata acgttagtgt 120
gaaagccact aagaacacat gataatggtt aactatgtcc atgaagacgt gtttgaatct 180
aattgaaaaa tccgatacac tagtatgttt tattcattta aacatattat ctgtgcaacg 240
tggtgctttc ggtttgatat gaaatggatt ccccgtattg cacgatattg attggttcaa 300
caacacaaat atgcatgact ctcacatgca tataggtata agagtcacta tgtaattttc 360
cttggtttca agttatgccc caaaataata cgtaatgtct tctaaaacca acatctaatg 420
tatgtctgta cgtgtacact gatgtatatc aactaaacaa cggacacatg tcttcataaa 480
aaaaccttaa acatgacaaa rcataagtga atagaggatg ataattattt tatttttatt 540
attagtacca cgggaaactt tgaaatcgat atactagcat gtttttcatt ttaggaaagg 600
agatactgcc atatatggag ccgagaccac cggtggggat tcaccgttac atatttgtac 660
ttttcaggca gaactcaccg gtgggtatga tggtgcagca gccgccttcg cgagccaact 720
tcagcacccg aatgttcgct ggacatctcg atcttggttt gcctgtggcc acagtttact 780
tcaacgccca gaaagagcca gcttcacgca gacgctgatg cacgcaacca aaataaaaga 840
gagagccttt tccggtttta cctaaaaacc ggaccggaaa gaaatatggg gtttatatat 900
caaaccatat tttgtatcat ccggttctcg actatatata tgtgtagatg catatacaat 960
tatacaaata tgtttatgtt tgtgtgttat attaagtggc t 1001
<210> 44
<211> 1001
<212> DNA
<213> Brassica napus
<400> 44
tacaaatgaa tttcactttt taactcactt tctaactcac ttgcttagat ataaaataac 60
gttagtgtga aagccactaa gaacacatga taatggttaa ctatgtccat gaagacgtgt 120
ttgaatctaa ttgaaaaatc cgatacacta gtatgtttta ttcatttaaa catattatct 180
gtgcaacgtg gtgctttcgg tttgatatga aatggattcc ccgtattgca cgatattgat 240
tggttcaaca acacaaatat gcatgactct cacatgcata taggtataag agtcactatg 300
taattttcct tggtttcaag ttatgcccca aaataatacg taatgtcttc taaaaccaac 360
atctaatgta tgtctgtacg tgtacactga tgtatatcaa ctaaacaacg gacacatgtc 420
ttcataaaaa aaccttaaac atgacaaagc ataagtgaat agaggatgat aattatttta 480
tttttattat tagtaccacg rgaaactttg aaatcgatat actagcatgt ttttcatttt 540
aggaaaggag atactgccat atatggagcc gagaccaccg gtggggattc accgttacat 600
atttgtactt ttcaggcaga actcaccggt gggtatgatg gtgcagcagc cgccttcgcg 660
agccaacttc agcacccgaa tgttcgctgg acatctcgat cttggtttgc ctgtggccac 720
agtttacttc aacgcccaga aagagccagc ttcacgcaga cgctgatgca cgcaaccaaa 780
ataaaagaga gagccttttc cggttttacc taaaaaccgg accggaaaga aatatggggt 840
ttatatatca aaccatattt tgtatcatcc ggttctcgac tatatatatg tgtagatgca 900
tatacaatta tacaaatatg tttatgtttg tgtgttatat taagtggctt gcgtataata 960
tatggttttc gttttctttt atctttaaat aaactaaaaa a 1001
<210> 45
<211> 1002
<212> DNA
<213> Brassica napus
<220>
<221> misc_feature
<222> (502)..(502)
<223> 其中n为插入/缺失 [+/T]
<400> 45
cgcccagaaa gagccagctt cacgcagacg ctgatgcacg caaccaaaat aaaagagaga 60
gccttttccg gttttaccta aaaaccggac cggaaagaaa tatggggttt atatatcaaa 120
ccatattttg tatcatccgg ttctcgacta tatatatgtg tagatgcata tacaattata 180
caaatatgtt tatgtttgtg tgttatatta agtggcttgc gtataatata tggttttcgt 240
tttcttttat ctttaaataa actaaaaaat aaaggtatgt atcaaattat aaagaaacaa 300
gaagagagga taaacaaaaa aaatgaaaat tctagtatac tgccttatta aaaaaaaaaa 360
atactattag ttcttaaacc gaaattcaga aatataaatg tcggttacaa atttgattcg 420
aacaaaatga accatttccc gtttaaataa actagtttca accaccacat ttaagttaaa 480
atatatcata ttattaattt tnaacccaaa actcaagaat acaaatgtca gttacaagct 540
taattccaac aaaatgattt aattctaact taaataaact agcttgcttg accgccggct 600
gacttaaata aactagcttt tcttgttgat cattagcctt cttctcctct gaacctatgt 660
agcttgtttt ctaaagattt tgatccacgc actctccttg aatttcaact acatcttctg 720
tggactcata tctttccttc tctctattct cagagtcttg ttttattcca gaatcatcaa 780
agcttggaca aatcgaatgt aacttcacgt tgatccactg aaaaatcttg ttcgtaagag 840
tttggtgttg aagtaacatc tagtgcttcg ccattggttt cttagagcat gattattgca 900
aagacccata ttaggggttc ttattatttt ttaatgcttt taagtacaaa aagtgattta 960
agagacaaat ttaagaaacc ctaacattta attgctccat tg 1002
<210> 46
<211> 1001
<212> DNA
<213> Brassica napus
<400> 46
agagcctttt ccggttttac ctaaaaaccg gaccggaaag aaatatgggg tttatatatc 60
aaaccatatt ttgtatcatc cggttctcga ctatatatat gtgtagatgc atatacaatt 120
atacaaatat gtttatgttt gtgtgttata ttaagtggct tgcgtataat atatggtttt 180
cgttttcttt tatctttaaa taaactaaaa aataaaggta tgtatcaaat tataaagaaa 240
caagaagaga ggataaacaa aaaaaatgaa aattctagta tactgcctta ttaaaaaaaa 300
aaaatactat tagttcttaa accgaaattc agaaatataa atgtcggtta caaatttgat 360
tcgaacaaaa tgaaccattt cccgtttaaa taaactagtt tcaaccacca catttaagtt 420
aaaatatatc atattattaa ttttaaccca aaactcaaga atacaaatgt cagttacaag 480
cttaattcca acaaaatgat ytaattctaa cttaaataaa ctagcttgct tgaccgccgg 540
ctgacttaaa taaactagct tttcttgttg atcattagcc ttcttctcct ctgaacctat 600
gtagcttgtt ttctaaagat tttgatccac gcactctcct tgaatttcaa ctacatcttc 660
tgtggactca tatctttcct tctctctatt ctcagagtct tgttttattc cagaatcatc 720
aaagcttgga caaatcgaat gtaacttcac gttgatccac tgaaaaatct tgttcgtaag 780
agtttggtgt tgaagtaaca tctagtgctt cgccattggt ttcttagagc atgattattg 840
caaagaccca tattaggggt tcttattatt ttttaatgct tttaagtaca aaaagtgatt 900
taagagacaa atttaagaaa ccctaacatt taattgctcc attgcaaggt tcttacaaca 960
ttactttcac tcttcttact tgatcttctt tagccaatgt t 1001
<210> 47
<211> 1001
<212> DNA
<213> Brassica napus
<400> 47
atatggggtt tatatatcaa accatatttt gtatcatccg gttctcgact atatatatgt 60
gtagatgcat atacaattat acaaatatgt ttatgtttgt gtgttatatt aagtggcttg 120
cgtataatat atggttttcg ttttctttta tctttaaata aactaaaaaa taaaggtatg 180
tatcaaatta taaagaaaca agaagagagg ataaacaaaa aaaatgaaaa ttctagtata 240
ctgccttatt aaaaaaaaaa aatactatta gttcttaaac cgaaattcag aaatataaat 300
gtcggttaca aatttgattc gaacaaaatg aaccatttcc cgtttaaata aactagtttc 360
aaccaccaca tttaagttaa aatatatcat attattaatt ttaacccaaa actcaagaat 420
acaaatgtca gttacaagct taattccaac aaaatgattt aattctaact taaataaact 480
agcttgcttg accgccggct racttaaata aactagcttt tcttgttgat cattagcctt 540
cttctcctct gaacctatgt agcttgtttt ctaaagattt tgatccacgc actctccttg 600
aatttcaact acatcttctg tggactcata tctttccttc tctctattct cagagtcttg 660
ttttattcca gaatcatcaa agcttggaca aatcgaatgt aacttcacgt tgatccactg 720
aaaaatcttg ttcgtaagag tttggtgttg aagtaacatc tagtgcttcg ccattggttt 780
cttagagcat gattattgca aagacccata ttaggggttc ttattatttt ttaatgcttt 840
taagtacaaa aagtgattta agagacaaat ttaagaaacc ctaacattta attgctccat 900
tgcaaggttc ttacaacatt actttcactc ttcttacttg atcttcttta gccaatgttg 960
ttccaatgtc aaagacgatg aaacagaaac aagaaaacct c 1001
<210> 48
<211> 1001
<212> DNA
<213> Brassica napus
<400> 48
taatatatgg ttttcgtttt cttttatctt taaataaact aaaaaataaa ggtatgtatc 60
aaattataaa gaaacaagaa gagaggataa acaaaaaaaa tgaaaattct agtatactgc 120
cttattaaaa aaaaaaaata ctattagttc ttaaaccgaa attcagaaat ataaatgtcg 180
gttacaaatt tgattcgaac aaaatgaacc atttcccgtt taaataaact agtttcaacc 240
accacattta agttaaaata tatcatatta ttaattttaa cccaaaactc aagaatacaa 300
atgtcagtta caagcttaat tccaacaaaa tgatttaatt ctaacttaaa taaactagct 360
tgcttgaccg ccggctgact taaataaact agcttttctt gttgatcatt agccttcttc 420
tcctctgaac ctatgtagct tgttttctaa agattttgat ccacgcactc tccttgaatt 480
tcaactacat cttctgtgga ytcatatctt tccttctctc tattctcaga gtcttgtttt 540
attccagaat catcaaagct tggacaaatc gaatgtaact tcacgttgat ccactgaaaa 600
atcttgttcg taagagtttg gtgttgaagt aacatctagt gcttcgccat tggtttctta 660
gagcatgatt attgcaaaga cccatattag gggttcttat tattttttaa tgcttttaag 720
tacaaaaagt gatttaagag acaaatttaa gaaaccctaa catttaattg ctccattgca 780
aggttcttac aacattactt tcactcttct tacttgatct tctttagcca atgttgttcc 840
aatgtcaaag acgatgaaac agaaacaaga aaacctcaat aaaatgaatc aataaccaga 900
accttgaaac atgaaacaaa acaacaaagc atatcattct cattactcaa acagaacaag 960
aacattaaca tgaaacagag acagaataag caaaacaacg t 1001
<210> 49
<211> 1001
<212> DNA
<213> Brassica napus
<400> 49
ataaagaaac aagaagagag gataaacaaa aaaaatgaaa attctagtat actgccttat 60
taaaaaaaaa aaatactatt agttcttaaa ccgaaattca gaaatataaa tgtcggttac 120
aaatttgatt cgaacaaaat gaaccatttc ccgtttaaat aaactagttt caaccaccac 180
atttaagtta aaatatatca tattattaat tttaacccaa aactcaagaa tacaaatgtc 240
agttacaagc ttaattccaa caaaatgatt taattctaac ttaaataaac tagcttgctt 300
gaccgccggc tgacttaaat aaactagctt ttcttgttga tcattagcct tcttctcctc 360
tgaacctatg tagcttgttt tctaaagatt ttgatccacg cactctcctt gaatttcaac 420
tacatcttct gtggactcat atctttcctt ctctctattc tcagagtctt gttttattcc 480
agaatcatca aagcttggac raatcgaatg taacttcacg ttgatccact gaaaaatctt 540
gttcgtaaga gtttggtgtt gaagtaacat ctagtgcttc gccattggtt tcttagagca 600
tgattattgc aaagacccat attaggggtt cttattattt tttaatgctt ttaagtacaa 660
aaagtgattt aagagacaaa tttaagaaac cctaacattt aattgctcca ttgcaaggtt 720
cttacaacat tactttcact cttcttactt gatcttcttt agccaatgtt gttccaatgt 780
caaagacgat gaaacagaaa caagaaaacc tcaataaaat gaatcaataa ccagaacctt 840
gaaacatgaa acaaaacaac aaagcatatc attctcatta ctcaaacaga acaagaacat 900
taacatgaaa cagagacaga ataagcaaaa caacgtgtag ttccccgtac gtcttgtgca 960
gcattgtaat tcttcacgga atcagtagta aaagttattt a 1001
<210> 50
<211> 1001
<212> DNA
<213> Brassica napus
<400> 50
attaaaaaaa aaaaatacta ttagttctta aaccgaaatt cagaaatata aatgtcggtt 60
acaaatttga ttcgaacaaa atgaaccatt tcccgtttaa ataaactagt ttcaaccacc 120
acatttaagt taaaatatat catattatta attttaaccc aaaactcaag aatacaaatg 180
tcagttacaa gcttaattcc aacaaaatga tttaattcta acttaaataa actagcttgc 240
ttgaccgccg gctgacttaa ataaactagc ttttcttgtt gatcattagc cttcttctcc 300
tctgaaccta tgtagcttgt tttctaaaga ttttgatcca cgcactctcc ttgaatttca 360
actacatctt ctgtggactc atatctttcc ttctctctat tctcagagtc ttgttttatt 420
ccagaatcat caaagcttgg acaaatcgaa tgtaacttca cgttgatcca ctgaaaaatc 480
ttgttcgtaa gagtttggtg ytgaagtaac atctagtgct tcgccattgg tttcttagag 540
catgattatt gcaaagaccc atattagggg ttcttattat tttttaatgc ttttaagtac 600
aaaaagtgat ttaagagaca aatttaagaa accctaacat ttaattgctc cattgcaagg 660
ttcttacaac attactttca ctcttcttac ttgatcttct ttagccaatg ttgttccaat 720
gtcaaagacg atgaaacaga aacaagaaaa cctcaataaa atgaatcaat aaccagaacc 780
ttgaaacatg aaacaaaaca acaaagcata tcattctcat tactcaaaca gaacaagaac 840
attaacatga aacagagaca gaataagcaa aacaacgtgt agttccccgt acgtcttgtg 900
cagcattgta attcttcacg gaatcagtag taaaagttat ttattaaacc gttctaacag 960
tagtaaacaa acatccaggt gctgtgaaaa aaatctatca a 1001
<210> 51
<211> 1001
<212> DNA
<213> Brassica napus
<400> 51
tactattagt tcttaaaccg aaattcagaa atataaatgt cggttacaaa tttgattcga 60
acaaaatgaa ccatttcccg tttaaataaa ctagtttcaa ccaccacatt taagttaaaa 120
tatatcatat tattaatttt aacccaaaac tcaagaatac aaatgtcagt tacaagctta 180
attccaacaa aatgatttaa ttctaactta aataaactag cttgcttgac cgccggctga 240
cttaaataaa ctagcttttc ttgttgatca ttagccttct tctcctctga acctatgtag 300
cttgttttct aaagattttg atccacgcac tctccttgaa tttcaactac atcttctgtg 360
gactcatatc tttccttctc tctattctca gagtcttgtt ttattccaga atcatcaaag 420
cttggacaaa tcgaatgtaa cttcacgttg atccactgaa aaatcttgtt cgtaagagtt 480
tggtgttgaa gtaacatcta stgcttcgcc attggtttct tagagcatga ttattgcaaa 540
gacccatatt aggggttctt attatttttt aatgctttta agtacaaaaa gtgatttaag 600
agacaaattt aagaaaccct aacatttaat tgctccattg caaggttctt acaacattac 660
tttcactctt cttacttgat cttctttagc caatgttgtt ccaatgtcaa agacgatgaa 720
acagaaacaa gaaaacctca ataaaatgaa tcaataacca gaaccttgaa acatgaaaca 780
aaacaacaaa gcatatcatt ctcattactc aaacagaaca agaacattaa catgaaacag 840
agacagaata agcaaaacaa cgtgtagttc cccgtacgtc ttgtgcagca ttgtaattct 900
tcacggaatc agtagtaaaa gttatttatt aaaccgttct aacagtagta aacaaacatc 960
caggtgctgt gaaaaaaatc tatcaattga aacaacgatc t 1001
<210> 52
<211> 1002
<212> DNA
<213> Brassica napus
<220>
<221> misc_feature
<222> (502)..(502)
<223> 其中n为插入/缺失 [+/A]
<400> 52
tggtggaaga agaagactca gctttgttgt tgttgcagag ataaccgcgg ctcagaggta 60
aggacgaaga agaatgcgac agctgtctca taagagacgt cccttgcgga gccaaattaa 120
gagacgtctt ctccgtgttt tgtatttttc attttttttt aatcacaata acctaagaga 180
catgcttagg agactgcgat aatggtgctc ttacagaccc cacgacagga gggcttgttc 240
actgtattct aattggtgga aaagaaagct acgggaaaga aatactcttg tgagcacata 300
tgttgttgtt caattctaag ggcagaaacg agttgctaga gttccatttt aaccagtttc 360
tgtacacctt taaacattgt aattgttgca tacacgccag cttattaaga aaatacaaat 420
tcaagtgtga ctactctgtt ccctcatttt gataggatta tcaaagatat cacattgaaa 480
caaaacagaa gcaactaaaa ancagtaaga tctctcacaa acaatataaa agatcgaaac 540
ctagaattga cataaaacaa aacattattg caaaatctca gtttgttgac aaaacaaaag 600
tgtgatagat aaaaaacaca tccaaggaga gagacagggc aaagagatgg gataaactag 660
taacggccaa taccccaaac tctgataact ccatcggtgt atccactgaa caaggtgctt 720
ccatccgcac tccagctcag gctagtgcag taaataacct gtccaaaaaa gtaaatgttt 780
aagtctagtt tctcaatcta ggcaagaaaa aaaaacagtt cttaccaaaa tataaaaaca 840
ttcaaaaatt gccatagata gaaaaatcta tatcagatct atcgattaca ttcacatatt 900
gataaaacac tagccaacaa tatactgcaa tctatggcaa acatgtataa aagtaaattc 960
agacaaaaga acacaagaca tttgagcatc cacatgaaaa ac 1002
<210> 53
<211> 1001
<212> DNA
<213> Brassica napus
<400> 53
gttgttgttg cagagataac cgcggctcag aggtaaggac gaagaagaat gcgacagctg 60
tctcataaga gacgtccctt gcggagccaa attaagagac gtcttctccg tgttttgtat 120
ttttcatttt tttttaatca caataaccta agagacatgc ttaggagact gcgataatgg 180
tgctcttaca gaccccacga caggagggct tgttcactgt attctaattg gtggaaaaga 240
aagctacggg aaagaaatac tcttgtgagc acatatgttg ttgttcaatt ctaagggcag 300
aaacgagttg ctagagttcc attttaacca gtttctgtac acctttaaac attgtaattg 360
ttgcatacac gccagcttat taagaaaata caaattcaag tgtgactact ctgttccctc 420
attttgatag gattatcaaa gatatcacat tgaaacaaaa cagaagcaac taaaaacagt 480
aagatctctc acaaacaata waaaagatcg aaacctagaa ttgacataaa acaaaacatt 540
attgcaaaat ctcagtttgt tgacaaaaca aaagtgtgat agataaaaaa cacatccaag 600
gagagagaca gggcaaagag atgggataaa ctagtaacgg ccaatacccc aaactctgat 660
aactccatcg gtgtatccac tgaacaaggt gcttccatcc gcactccagc tcaggctagt 720
gcagtaaata acctgtccaa aaaagtaaat gtttaagtct agtttctcaa tctaggcaag 780
aaaaaaaaac agttcttacc aaaatataaa aacattcaaa aattgccata gatagaaaaa 840
tctatatcag atctatcgat tacattcaca tattgataaa acactagcca acaatatact 900
gcaatctatg gcaaacatgt ataaaagtaa attcagacaa aagaacacaa gacatttgag 960
catccacatg aaaaacaaac atatttacaa aacataaaca a 1001
<210> 54
<211> 1001
<212> DNA
<213> Brassica napus
<400> 54
agagacgtct tctccgtgtt ttgtattttt catttttttt taatcacaat aacctaagag 60
acatgcttag gagactgcga taatggtgct cttacagacc ccacgacagg agggcttgtt 120
cactgtattc taattggtgg aaaagaaagc tacgggaaag aaatactctt gtgagcacat 180
atgttgttgt tcaattctaa gggcagaaac gagttgctag agttccattt taaccagttt 240
ctgtacacct ttaaacattg taattgttgc atacacgcca gcttattaag aaaatacaaa 300
ttcaagtgtg actactctgt tccctcattt tgataggatt atcaaagata tcacattgaa 360
acaaaacaga agcaactaaa aacagtaaga tctctcacaa acaatataaa agatcgaaac 420
ctagaattga cataaaacaa aacattattg caaaatctca gtttgttgac aaaacaaaag 480
tgtgatagat aaaaaacaca wccaaggaga gagacagggc aaagagatgg gataaactag 540
taacggccaa taccccaaac tctgataact ccatcggtgt atccactgaa caaggtgctt 600
ccatccgcac tccagctcag gctagtgcag taaataacct gtccaaaaaa gtaaatgttt 660
aagtctagtt tctcaatcta ggcaagaaaa aaaaacagtt cttaccaaaa tataaaaaca 720
ttcaaaaatt gccatagata gaaaaatcta tatcagatct atcgattaca ttcacatatt 780
gataaaacac tagccaacaa tatactgcaa tctatggcaa acatgtataa aagtaaattc 840
agacaaaaga acacaagaca tttgagcatc cacatgaaaa acaaacatat ttacaaaaca 900
taaacaagga tataaaacca caatcataac cataagcatg catcgtttat ttgcatttca 960
atagatcaat ccaaaagaac catttctata aacactatcc t 1001
<210> 55
<211> 1001
<212> DNA
<213> Brassica napus
<400> 55
gataatggtg ctcttacaga ccccacgaca ggagggcttg ttcactgtat tctaattggt 60
ggaaaagaaa gctacgggaa agaaatactc ttgtgagcac atatgttgtt gttcaattct 120
aagggcagaa acgagttgct agagttccat tttaaccagt ttctgtacac ctttaaacat 180
tgtaattgtt gcatacacgc cagcttatta agaaaataca aattcaagtg tgactactct 240
gttccctcat tttgatagga ttatcaaaga tatcacattg aaacaaaaca gaagcaacta 300
aaaacagtaa gatctctcac aaacaatata aaagatcgaa acctagaatt gacataaaac 360
aaaacattat tgcaaaatct cagtttgttg acaaaacaaa agtgtgatag ataaaaaaca 420
catccaagga gagagacagg gcaaagagat gggataaact agtaacggcc aataccccaa 480
actctgataa ctccatcggt rtatccactg aacaaggtgc ttccatccgc actccagctc 540
aggctagtgc agtaaataac ctgtccaaaa aagtaaatgt ttaagtctag tttctcaatc 600
taggcaagaa aaaaaaacag ttcttaccaa aatataaaaa cattcaaaaa ttgccataga 660
tagaaaaatc tatatcagat ctatcgatta cattcacata ttgataaaac actagccaac 720
aatatactgc aatctatggc aaacatgtat aaaagtaaat tcagacaaaa gaacacaaga 780
catttgagca tccacatgaa aaacaaacat atttacaaaa cataaacaag gatataaaac 840
cacaatcata accataagca tgcatcgttt atttgcattt caatagatca atccaaaaga 900
accatttcta taaacactat cctctatcaa cagtttacct aaactaaaga aatattatcc 960
atacaataag catcaaactt gcatgtcttt gtacctttct t 1001
<210> 56
<211> 1001
<212> DNA
<213> Brassica napus
<400> 56
tgtattctaa ttggtggaaa agaaagctac gggaaagaaa tactcttgtg agcacatatg 60
ttgttgttca attctaaggg cagaaacgag ttgctagagt tccattttaa ccagtttctg 120
tacaccttta aacattgtaa ttgttgcata cacgccagct tattaagaaa atacaaattc 180
aagtgtgact actctgttcc ctcattttga taggattatc aaagatatca cattgaaaca 240
aaacagaagc aactaaaaac agtaagatct ctcacaaaca atataaaaga tcgaaaccta 300
gaattgacat aaaacaaaac attattgcaa aatctcagtt tgttgacaaa acaaaagtgt 360
gatagataaa aaacacatcc aaggagagag acagggcaaa gagatgggat aaactagtaa 420
cggccaatac cccaaactct gataactcca tcggtgtatc cactgaacaa ggtgcttcca 480
tccgcactcc agctcaggct rgtgcagtaa ataacctgtc caaaaaagta aatgtttaag 540
tctagtttct caatctaggc aagaaaaaaa aacagttctt accaaaatat aaaaacattc 600
aaaaattgcc atagatagaa aaatctatat cagatctatc gattacattc acatattgat 660
aaaacactag ccaacaatat actgcaatct atggcaaaca tgtataaaag taaattcaga 720
caaaagaaca caagacattt gagcatccac atgaaaaaca aacatattta caaaacataa 780
acaaggatat aaaaccacaa tcataaccat aagcatgcat cgtttatttg catttcaata 840
gatcaatcca aaagaaccat ttctataaac actatcctct atcaacagtt tacctaaact 900
aaagaaatat tatccataca ataagcatca aacttgcatg tctttgtacc tttcttttgg 960
tggcagcagt accacttcca tcggacttct cagcctcagc c 1001
<210> 57
<211> 1001
<212> DNA
<213> Brassica napus
<400> 57
gggcagaaac gagttgctag agttccattt taaccagttt ctgtacacct ttaaacattg 60
taattgttgc atacacgcca gcttattaag aaaatacaaa ttcaagtgtg actactctgt 120
tccctcattt tgataggatt atcaaagata tcacattgaa acaaaacaga agcaactaaa 180
aacagtaaga tctctcacaa acaatataaa agatcgaaac ctagaattga cataaaacaa 240
aacattattg caaaatctca gtttgttgac aaaacaaaag tgtgatagat aaaaaacaca 300
tccaaggaga gagacagggc aaagagatgg gataaactag taacggccaa taccccaaac 360
tctgataact ccatcggtgt atccactgaa caaggtgctt ccatccgcac tccagctcag 420
gctagtgcag taaataacct gtccaaaaaa gtaaatgttt aagtctagtt tctcaatcta 480
ggcaagaaaa aaaaacagtt sttaccaaaa tataaaaaca ttcaaaaatt gccatagata 540
gaaaaatcta tatcagatct atcgattaca ttcacatatt gataaaacac tagccaacaa 600
tatactgcaa tctatggcaa acatgtataa aagtaaattc agacaaaaga acacaagaca 660
tttgagcatc cacatgaaaa acaaacatat ttacaaaaca taaacaagga tataaaacca 720
caatcataac cataagcatg catcgtttat ttgcatttca atagatcaat ccaaaagaac 780
catttctata aacactatcc tctatcaaca gtttacctaa actaaagaaa tattatccat 840
acaataagca tcaaacttgc atgtctttgt acctttcttt tggtggcagc agtaccactt 900
ccatcggact tctcagcctc agccttgaga tcaaccttca agtcctcaac aacactcttg 960
ctctcaagat cccaaatctt aataccctgc tcagtcgcag c 1001
<210> 58
<211> 1001
<212> DNA
<213> Brassica napus
<400> 58
agaaacgagt tgctagagtt ccattttaac cagtttctgt acacctttaa acattgtaat 60
tgttgcatac acgccagctt attaagaaaa tacaaattca agtgtgacta ctctgttccc 120
tcattttgat aggattatca aagatatcac attgaaacaa aacagaagca actaaaaaca 180
gtaagatctc tcacaaacaa tataaaagat cgaaacctag aattgacata aaacaaaaca 240
ttattgcaaa atctcagttt gttgacaaaa caaaagtgtg atagataaaa aacacatcca 300
aggagagaga cagggcaaag agatgggata aactagtaac ggccaatacc ccaaactctg 360
ataactccat cggtgtatcc actgaacaag gtgcttccat ccgcactcca gctcaggcta 420
gtgcagtaaa taacctgtcc aaaaaagtaa atgtttaagt ctagtttctc aatctaggca 480
agaaaaaaaa acagttctta mcaaaatata aaaacattca aaaattgcca tagatagaaa 540
aatctatatc agatctatcg attacattca catattgata aaacactagc caacaatata 600
ctgcaatcta tggcaaacat gtataaaagt aaattcagac aaaagaacac aagacatttg 660
agcatccaca tgaaaaacaa acatatttac aaaacataaa caaggatata aaaccacaat 720
cataaccata agcatgcatc gtttatttgc atttcaatag atcaatccaa aagaaccatt 780
tctataaaca ctatcctcta tcaacagttt acctaaacta aagaaatatt atccatacaa 840
taagcatcaa acttgcatgt ctttgtacct ttcttttggt ggcagcagta ccacttccat 900
cggacttctc agcctcagcc ttgagatcaa ccttcaagtc ctcaacaaca ctcttgctct 960
caagatccca aatcttaata ccctgctcag tcgcagcaca a 1001
<210> 59
<211> 1001
<212> DNA
<213> Brassica napus
<400> 59
gagttgctag agttccattt taaccagttt ctgtacacct ttaaacattg taattgttgc 60
atacacgcca gcttattaag aaaatacaaa ttcaagtgtg actactctgt tccctcattt 120
tgataggatt atcaaagata tcacattgaa acaaaacaga agcaactaaa aacagtaaga 180
tctctcacaa acaatataaa agatcgaaac ctagaattga cataaaacaa aacattattg 240
caaaatctca gtttgttgac aaaacaaaag tgtgatagat aaaaaacaca tccaaggaga 300
gagacagggc aaagagatgg gataaactag taacggccaa taccccaaac tctgataact 360
ccatcggtgt atccactgaa caaggtgctt ccatccgcac tccagctcag gctagtgcag 420
taaataacct gtccaaaaaa gtaaatgttt aagtctagtt tctcaatcta ggcaagaaaa 480
aaaaacagtt cttaccaaaa yataaaaaca ttcaaaaatt gccatagata gaaaaatcta 540
tatcagatct atcgattaca ttcacatatt gataaaacac tagccaacaa tatactgcaa 600
tctatggcaa acatgtataa aagtaaattc agacaaaaga acacaagaca tttgagcatc 660
cacatgaaaa acaaacatat ttacaaaaca taaacaagga tataaaacca caatcataac 720
cataagcatg catcgtttat ttgcatttca atagatcaat ccaaaagaac catttctata 780
aacactatcc tctatcaaca gtttacctaa actaaagaaa tattatccat acaataagca 840
tcaaacttgc atgtctttgt acctttcttt tggtggcagc agtaccactt ccatcggact 900
tctcagcctc agccttgaga tcaaccttca agtcctcaac aacactcttg ctctcaagat 960
cccaaatctt aataccctgc tcagtcgcag cacaaagcca g 1001
<210> 60
<211> 1001
<212> DNA
<213> Brassica napus
<400> 60
cacattgaaa caaaacagaa gcaactaaaa acagtaagat ctctcacaaa caatataaaa 60
gatcgaaacc tagaattgac ataaaacaaa acattattgc aaaatctcag tttgttgaca 120
aaacaaaagt gtgatagata aaaaacacat ccaaggagag agacagggca aagagatggg 180
ataaactagt aacggccaat accccaaact ctgataactc catcggtgta tccactgaac 240
aaggtgcttc catccgcact ccagctcagg ctagtgcagt aaataacctg tccaaaaaag 300
taaatgttta agtctagttt ctcaatctag gcaagaaaaa aaaacagttc ttaccaaaat 360
ataaaaacat tcaaaaattg ccatagatag aaaaatctat atcagatcta tcgattacat 420
tcacatattg ataaaacact agccaacaat atactgcaat ctatggcaaa catgtataaa 480
agtaaattca gacaaaagaa sacaagacat ttgagcatcc acatgaaaaa caaacatatt 540
tacaaaacat aaacaaggat ataaaaccac aatcataacc ataagcatgc atcgtttatt 600
tgcatttcaa tagatcaatc caaaagaacc atttctataa acactatcct ctatcaacag 660
tttacctaaa ctaaagaaat attatccata caataagcat caaacttgca tgtctttgta 720
cctttctttt ggtggcagca gtaccacttc catcggactt ctcagcctca gccttgagat 780
caaccttcaa gtcctcaaca acactcttgc tctcaagatc ccaaatctta ataccctgct 840
cagtcgcagc acaaagccag tacctattag gactaaagca aagagcgtga atcacagagt 900
tggcttcaag agagtaaagc ttcttcccct cagccaaatc ccagagcaaa acgacaccgt 960
ctttgcctcc actagcacac agagaaccat caggcgacac a 1001
<210> 61
<211> 1001
<212> DNA
<213> Brassica napus
<400> 61
cattgaaaca aaacagaagc aactaaaaac agtaagatct ctcacaaaca atataaaaga 60
tcgaaaccta gaattgacat aaaacaaaac attattgcaa aatctcagtt tgttgacaaa 120
acaaaagtgt gatagataaa aaacacatcc aaggagagag acagggcaaa gagatgggat 180
aaactagtaa cggccaatac cccaaactct gataactcca tcggtgtatc cactgaacaa 240
ggtgcttcca tccgcactcc agctcaggct agtgcagtaa ataacctgtc caaaaaagta 300
aatgtttaag tctagtttct caatctaggc aagaaaaaaa aacagttctt accaaaatat 360
aaaaacattc aaaaattgcc atagatagaa aaatctatat cagatctatc gattacattc 420
acatattgat aaaacactag ccaacaatat actgcaatct atggcaaaca tgtataaaag 480
taaattcaga caaaagaaca yaagacattt gagcatccac atgaaaaaca aacatattta 540
caaaacataa acaaggatat aaaaccacaa tcataaccat aagcatgcat cgtttatttg 600
catttcaata gatcaatcca aaagaaccat ttctataaac actatcctct atcaacagtt 660
tacctaaact aaagaaatat tatccataca ataagcatca aacttgcatg tctttgtacc 720
tttcttttgg tggcagcagt accacttcca tcggacttct cagcctcagc cttgagatca 780
accttcaagt cctcaacaac actcttgctc tcaagatccc aaatcttaat accctgctca 840
gtcgcagcac aaagccagta cctattagga ctaaagcaaa gagcgtgaat cacagagttg 900
gcttcaagag agtaaagctt cttcccctca gccaaatccc agagcaaaac gacaccgtct 960
ttgcctccac tagcacacag agaaccatca ggcgacacag c 1001
<210> 62
<211> 1002
<212> DNA
<213> Brassica napus
<220>
<221> misc_feature
<222> (502)..(502)
<223> 其中n为插入/缺失 [+/A]
<400> 62
aaaaacacat ccaaggagag agacagggca aagagatggg ataaactagt aacggccaat 60
accccaaact ctgataactc catcggtgta tccactgaac aaggtgcttc catccgcact 120
ccagctcagg ctagtgcagt aaataacctg tccaaaaaag taaatgttta agtctagttt 180
ctcaatctag gcaagaaaaa aaaacagttc ttaccaaaat ataaaaacat tcaaaaattg 240
ccatagatag aaaaatctat atcagatcta tcgattacat tcacatattg ataaaacact 300
agccaacaat atactgcaat ctatggcaaa catgtataaa agtaaattca gacaaaagaa 360
cacaagacat ttgagcatcc acatgaaaaa caaacatatt tacaaaacat aaacaaggat 420
ataaaaccac aatcataacc ataagcatgc atcgtttatt tgcatttcaa tagatcaatc 480
caaaagaacc atttctataa ancactatcc tctatcaaca gtttacctaa actaaagaaa 540
tattatccat acaataagca tcaaacttgc atgtctttgt acctttcttt tggtggcagc 600
agtaccactt ccatcggact tctcagcctc agccttgaga tcaaccttca agtcctcaac 660
aacactcttg ctctcaagat cccaaatctt aataccctgc tcagtcgcag cacaaagcca 720
gtacctatta ggactaaagc aaagagcgtg aatcacagag ttggcttcaa gagagtaaag 780
cttcttcccc tcagccaaat cccagagcaa aacgacaccg tctttgcctc cactagcaca 840
cagagaacca tcaggcgaca cagccacagt actaacgtaa ccagtgtgac cagcaagagt 900
cgacctcagc ttacagttcg acaagttcca aactttcacg gtcttgtccc acgacgccga 960
cacaatcgtc ggctggagcg tgttgggact gaacctaacg ca 1002
<210> 63
<211> 1001
<212> DNA
<213> Brassica napus
<400> 63
ggccaatacc ccaaactctg ataactccat cggtgtatcc actgaacaag gtgcttccat 60
ccgcactcca gctcaggcta gtgcagtaaa taacctgtcc aaaaaagtaa atgtttaagt 120
ctagtttctc aatctaggca agaaaaaaaa acagttctta ccaaaatata aaaacattca 180
aaaattgcca tagatagaaa aatctatatc agatctatcg attacattca catattgata 240
aaacactagc caacaatata ctgcaatcta tggcaaacat gtataaaagt aaattcagac 300
aaaagaacac aagacatttg agcatccaca tgaaaaacaa acatatttac aaaacataaa 360
caaggatata aaaccacaat cataaccata agcatgcatc gtttatttgc atttcaatag 420
atcaatccaa aagaaccatt tctataaaca ctatcctcta tcaacagttt acctaaacta 480
aagaaatatt atccatacaa waagcatcaa acttgcatgt ctttgtacct ttcttttggt 540
ggcagcagta ccacttccat cggacttctc agcctcagcc ttgagatcaa ccttcaagtc 600
ctcaacaaca ctcttgctct caagatccca aatcttaata ccctgctcag tcgcagcaca 660
aagccagtac ctattaggac taaagcaaag agcgtgaatc acagagttgg cttcaagaga 720
gtaaagcttc ttcccctcag ccaaatccca gagcaaaacg acaccgtctt tgcctccact 780
agcacacaga gaaccatcag gcgacacagc cacagtacta acgtaaccag tgtgaccagc 840
aagagtcgac ctcagcttac agttcgacaa gttccaaact ttcacggtct tgtcccacga 900
cgccgacaca atcgtcggct ggagcgtgtt gggactgaac ctaacgcagc tgacccagtc 960
acggtgccct tcgcctcctt cggagattgt gtacttacac t 1001
<210> 64
<211> 1001
<212> DNA
<213> Brassica napus
<400> 64
tccaaaaaag taaatgttta agtctagttt ctcaatctag gcaagaaaaa aaaacagttc 60
ttaccaaaat ataaaaacat tcaaaaattg ccatagatag aaaaatctat atcagatcta 120
tcgattacat tcacatattg ataaaacact agccaacaat atactgcaat ctatggcaaa 180
catgtataaa agtaaattca gacaaaagaa cacaagacat ttgagcatcc acatgaaaaa 240
caaacatatt tacaaaacat aaacaaggat ataaaaccac aatcataacc ataagcatgc 300
atcgtttatt tgcatttcaa tagatcaatc caaaagaacc atttctataa acactatcct 360
ctatcaacag tttacctaaa ctaaagaaat attatccata caataagcat caaacttgca 420
tgtctttgta cctttctttt ggtggcagca gtaccacttc catcggactt ctcagcctca 480
gccttgagat caaccttcaa rtcctcaaca acactcttgc tctcaagatc ccaaatctta 540
ataccctgct cagtcgcagc acaaagccag tacctattag gactaaagca aagagcgtga 600
atcacagagt tggcttcaag agagtaaagc ttcttcccct cagccaaatc ccagagcaaa 660
acgacaccgt ctttgcctcc actagcacac agagaaccat caggcgacac agccacagta 720
ctaacgtaac cagtgtgacc agcaagagtc gacctcagct tacagttcga caagttccaa 780
actttcacgg tcttgtccca cgacgccgac acaatcgtcg gctggagcgt gttgggactg 840
aacctaacgc agctgaccca gtcacggtgc ccttcgcctc cttcggagat tgtgtactta 900
cactccccca gagtgttcca gagcttgatc gtgcggtcac gggaggccga cacgatctga 960
cggttgtcga gcgagaaggc cacggagagg acgtctttgg t 1001
<210> 65
<211> 1001
<212> DNA
<213> Brassica napus
<400> 65
tacctaaact aaagaaatat tatccataca ataagcatca aacttgcatg tctttgtacc 60
tttcttttgg tggcagcagt accacttcca tcggacttct cagcctcagc cttgagatca 120
accttcaagt cctcaacaac actcttgctc tcaagatccc aaatcttaat accctgctca 180
gtcgcagcac aaagccagta cctattagga ctaaagcaaa gagcgtgaat cacagagttg 240
gcttcaagag agtaaagctt cttcccctca gccaaatccc agagcaaaac gacaccgtct 300
ttgcctccac tagcacacag agaaccatca ggcgacacag ccacagtact aacgtaacca 360
gtgtgaccag caagagtcga cctcagctta cagttcgaca agttccaaac tttcacggtc 420
ttgtcccacg acgccgacac aatcgtcggc tggagcgtgt tgggactgaa cctaacgcag 480
ctgacccagt cacggtgccc ytcgcctcct tcggagattg tgtacttaca ctcccccaga 540
gtgttccaga gcttgatcgt gcggtcacgg gaggccgaca cgatctgacg gttgtcgagc 600
gagaaggcca cggagaggac gtctttggtg tgtccgacga atctgcgagt ggagacgccg 660
gcggcgaggt cccagagacg aagctcgccg tcccagctgc cggaaagcgc gaattggccg 720
tcggaggaga ggacgacgtc ttcgacgaag tgggagtggc cggtgaggcg tctctgggct 780
acgccgtagg atttgtcgtc ctttgtgagt ttccagacga tgatggattt gtcgcgggaa 840
gcggacacga tggtgtcgga gttgtcgatg ggggtggcga ttgcggtgac catgtcggtg 900
tgagcacgca tggtgccctt gaggacgagt ccttccgcca ttgtcgaagt ctggtgaagc 960
ttagggttat cagtttctcg ggggaggcgg agattcagac g 1001
<210> 66
<211> 1001
<212> DNA
<213> Brassica napus
<400> 66
acaataagca tcaaacttgc atgtctttgt acctttcttt tggtggcagc agtaccactt 60
ccatcggact tctcagcctc agccttgaga tcaaccttca agtcctcaac aacactcttg 120
ctctcaagat cccaaatctt aataccctgc tcagtcgcag cacaaagcca gtacctatta 180
ggactaaagc aaagagcgtg aatcacagag ttggcttcaa gagagtaaag cttcttcccc 240
tcagccaaat cccagagcaa aacgacaccg tctttgcctc cactagcaca cagagaacca 300
tcaggcgaca cagccacagt actaacgtaa ccagtgtgac cagcaagagt cgacctcagc 360
ttacagttcg acaagttcca aactttcacg gtcttgtccc acgacgccga cacaatcgtc 420
ggctggagcg tgttgggact gaacctaacg cagctgaccc agtcacggtg cccttcgcct 480
ccttcggaga ttgtgtactt rcactccccc agagtgttcc agagcttgat cgtgcggtca 540
cgggaggccg acacgatctg acggttgtcg agcgagaagg ccacggagag gacgtctttg 600
gtgtgtccga cgaatctgcg agtggagacg ccggcggcga ggtcccagag acgaagctcg 660
ccgtcccagc tgccggaaag cgcgaattgg ccgtcggagg agaggacgac gtcttcgacg 720
aagtgggagt ggccggtgag gcgtctctgg gctacgccgt aggatttgtc gtcctttgtg 780
agtttccaga cgatgatgga tttgtcgcgg gaagcggaca cgatggtgtc ggagttgtcg 840
atgggggtgg cgattgcggt gaccatgtcg gtgtgagcac gcatggtgcc cttgaggacg 900
agtccttccg ccattgtcga agtctggtga agcttagggt tatcagtttc tcgggggagg 960
cggagattca gacgaaatgg cgtcgagagg taagattcag t 1001
<210> 67
<211> 1001
<212> DNA
<213> Brassica napus
<400> 67
tcagcctcag ccttgagatc aaccttcaag tcctcaacaa cactcttgct ctcaagatcc 60
caaatcttaa taccctgctc agtcgcagca caaagccagt acctattagg actaaagcaa 120
agagcgtgaa tcacagagtt ggcttcaaga gagtaaagct tcttcccctc agccaaatcc 180
cagagcaaaa cgacaccgtc tttgcctcca ctagcacaca gagaaccatc aggcgacaca 240
gccacagtac taacgtaacc agtgtgacca gcaagagtcg acctcagctt acagttcgac 300
aagttccaaa ctttcacggt cttgtcccac gacgccgaca caatcgtcgg ctggagcgtg 360
ttgggactga acctaacgca gctgacccag tcacggtgcc cttcgcctcc ttcggagatt 420
gtgtacttac actcccccag agtgttccag agcttgatcg tgcggtcacg ggaggccgac 480
acgatctgac ggttgtcgag sgagaaggcc acggagagga cgtctttggt gtgtccgacg 540
aatctgcgag tggagacgcc ggcggcgagg tcccagagac gaagctcgcc gtcccagctg 600
ccggaaagcg cgaattggcc gtcggaggag aggacgacgt cttcgacgaa gtgggagtgg 660
ccggtgaggc gtctctgggc tacgccgtag gatttgtcgt cctttgtgag tttccagacg 720
atgatggatt tgtcgcggga agcggacacg atggtgtcgg agttgtcgat gggggtggcg 780
attgcggtga ccatgtcggt gtgagcacgc atggtgccct tgaggacgag tccttccgcc 840
attgtcgaag tctggtgaag cttagggtta tcagtttctc gggggaggcg gagattcaga 900
cgaaatggcg tcgagaggta agattcagtt tatatcagag acaacacaat ggattagggt 960
ttacttttat tgggctttga tgattaagtt taatgaatgg g 1001
<210> 68
<211> 1001
<212> DNA
<213> Brassica napus
<400> 68
tacctattag gactaaagca aagagcgtga atcacagagt tggcttcaag agagtaaagc 60
ttcttcccct cagccaaatc ccagagcaaa acgacaccgt ctttgcctcc actagcacac 120
agagaaccat caggcgacac agccacagta ctaacgtaac cagtgtgacc agcaagagtc 180
gacctcagct tacagttcga caagttccaa actttcacgg tcttgtccca cgacgccgac 240
acaatcgtcg gctggagcgt gttgggactg aacctaacgc agctgaccca gtcacggtgc 300
ccttcgcctc cttcggagat tgtgtactta cactccccca gagtgttcca gagcttgatc 360
gtgcggtcac gggaggccga cacgatctga cggttgtcga gcgagaaggc cacggagagg 420
acgtctttgg tgtgtccgac gaatctgcga gtggagacgc cggcggcgag gtcccagaga 480
cgaagctcgc cgtcccagct kccggaaagc gcgaattggc cgtcggagga gaggacgacg 540
tcttcgacga agtgggagtg gccggtgagg cgtctctggg ctacgccgta ggatttgtcg 600
tcctttgtga gtttccagac gatgatggat ttgtcgcggg aagcggacac gatggtgtcg 660
gagttgtcga tgggggtggc gattgcggtg accatgtcgg tgtgagcacg catggtgccc 720
ttgaggacga gtccttccgc cattgtcgaa gtctggtgaa gcttagggtt atcagtttct 780
cgggggaggc ggagattcag acgaaatggc gtcgagaggt aagattcagt ttatatcaga 840
gacaacacaa tggattaggg tttactttta ttgggctttg atgattaagt ttaatgaatg 900
ggtcttgcgt aaatgggctt tttttgtact ggtaagagtt tttttggttt tacttggtgt 960
taagaattta ccaatgttcg agaatcggta actagactag c 1001
<210> 69
<211> 1001
<212> DNA
<213> Brassica napus
<400> 69
cctccactag cacacagaga accatcaggc gacacagcca cagtactaac gtaaccagtg 60
tgaccagcaa gagtcgacct cagcttacag ttcgacaagt tccaaacttt cacggtcttg 120
tcccacgacg ccgacacaat cgtcggctgg agcgtgttgg gactgaacct aacgcagctg 180
acccagtcac ggtgcccttc gcctccttcg gagattgtgt acttacactc ccccagagtg 240
ttccagagct tgatcgtgcg gtcacgggag gccgacacga tctgacggtt gtcgagcgag 300
aaggccacgg agaggacgtc tttggtgtgt ccgacgaatc tgcgagtgga gacgccggcg 360
gcgaggtccc agagacgaag ctcgccgtcc cagctgccgg aaagcgcgaa ttggccgtcg 420
gaggagagga cgacgtcttc gacgaagtgg gagtggccgg tgaggcgtct ctgggctacg 480
ccgtaggatt tgtcgtcctt kgtgagtttc cagacgatga tggatttgtc gcgggaagcg 540
gacacgatgg tgtcggagtt gtcgatgggg gtggcgattg cggtgaccat gtcggtgtga 600
gcacgcatgg tgcccttgag gacgagtcct tccgccattg tcgaagtctg gtgaagctta 660
gggttatcag tttctcgggg gaggcggaga ttcagacgaa atggcgtcga gaggtaagat 720
tcagtttata tcagagacaa cacaatggat tagggtttac ttttattggg ctttgatgat 780
taagtttaat gaatgggtct tgcgtaaatg ggcttttttt gtactggtaa gagttttttt 840
ggttttactt ggtgttaaga atttaccaat gttcgagaat cggtaactag actagcgcct 900
agacggatta ttcagaacct aaacgagatc tagatattaa cgaattatta atttatttta 960
tatttatata aaacatttta atttttaatt ataaaattat t 1001
<210> 70
<211> 1001
<212> DNA
<213> Brassica napus
<400> 70
gattcggcag gttattcctg aagatgacaa gcttaatcat cttttttttg tttcgcattg 60
gtgctaaatg tttgtgttga tatatgctgc agaagaaaac aacggagttg ttatcccgga 120
agctcataac tctgatgaag ttgagaaatt ggatacagca gaagaaggtt cattgtagca 180
tgagcatgac atttttttgt ttatgctttc tttgttacac tgtttaaaat ttggctatct 240
gtgggtcgtt tattagatgc gtaagacatt aactagtggt taagggaggg ttatactatc 300
atgagattgg atactgattc taatactagg tatcatcctt gctttgcaga cctgaaagac 360
aaggtggaag agtcagcacc ggttcctgat gagcaacaag gttcaatctt ttatcttctc 420
tttctgtctc atttttctta acgtttagtt taatatatct gagtttggcg agttttattt 480
atttttgctt gtcattggtg rtttcagtgt ccgaggatca tgatcaagaa gtgcaccatg 540
cagtgcataa cccagcgaaa ggttcataga tctctcattc tacagtcttc tcatcttatg 600
agcgttcatg ttgtctggtt gaagatttat ttatctcttt cgtatatttt atccattcag 660
ctaaagagaa ggcagcccaa gagaaggctg ccaaagagga agctgaagaa gaggcagaag 720
caaacaagaa aagacacttg aacgtggtgt tcatcgggca tgttggtatg gctacttgtt 780
gatttctttt catcagctct actttcataa tagatatatc atctgcactt gtttagactc 840
agggcttaaa agcgtatgta acacattctt gaaattagga tcatgagctt ttagtcggtg 900
tgttttaact tttaagcttc ttgatttaat ctttacatgg tcaccttttc aattgtagat 960
gctggaaagt ctacaattgg aggacaaatt ctcttcctta g 1001
<210> 71
<211> 121
<212> DNA
<213> Brassica napus
<400> 71
acrtttagtt taatatatct gagtttggcg agttttattt atttttgctt gtcattggtg 60
rtttcagykt ccgaggatca tgatcwagaa gtgcaccatg cagtgcataa cccagcgaaa 120
g 121
<210> 72
<211> 1001
<212> DNA
<213> Brassica napus
<400> 72
gaacgtggtg ttcatcgggc atgttggtat ggctacttgt tgatttcttt tcatcagctc 60
tactttcata atagatatat catctgcact tgtttagact cagggcttaa aagcgtatgt 120
aacacattct tgaaattagg atcatgagct tttagtcggt gtgttttaac ttttaagctt 180
cttgatttaa tctttacatg gtcacctttt caattgtaga tgctggaaag tctacaattg 240
gaggacaaat tctcttcctt agcggtcagg tggacgaccg acaaatccaa aagtatgaaa 300
aagaagcaaa agaaaaaagt agagaaagct ggtgggtggt tctgattaat ttgaaatgat 360
aaggaattct tttgttctct ttttcttttt gtttttataa acttgttatc gttctgtatg 420
ctaggtatat ggcttatata atggatacaa atgaagaaga gagggcgaag gtatttcctg 480
attttttatt tatgtttctt wgtgtgctat aaatgtgtca gagatgataa gaattcacgt 540
gtctgtagaa ttttcagcaa tgtttatggt tgaatttaac ttagctaact gttatgactt 600
acttgctaca ttgaacaggg caaaacagtt gaagttggaa gggctcattt tgaaactgcg 660
agtacgagat ttaccatttt ggatgctccg gtaagagacc aacttaaaag aataattttt 720
tgttcacttg tctttatgag agtattttgt tctaattctt ttgcgccttt tttgaacttg 780
tagggtcaca agagttatgt accaaatatg attagtggag catctcaagc ggacattggt 840
gtactggtaa gttattatct taatttggtc ggagtcgtta ctgtgtagtg tgcgtctttg 900
gtaggaagct tattaatttt catgtccttg tccctctgtt gtaggtgatt tcggctcgta 960
aaggtgaatt tgaaacggga tatgagaggg gtgggcagac c 1001
<210> 73
<211> 1001
<212> DNA
<213> Brassica napus
<400> 73
ctacttgttg atttcttttc atcagctcta ctttcataat agatatatca tctgcacttg 60
tttagactca gggcttaaaa gcgtatgtaa cacattcttg aaattaggat catgagcttt 120
tagtcggtgt gttttaactt ttaagcttct tgatttaatc tttacatggt caccttttca 180
attgtagatg ctggaaagtc tacaattgga ggacaaattc tcttccttag cggtcaggtg 240
gacgaccgac aaatccaaaa gtatgaaaaa gaagcaaaag aaaaaagtag agaaagctgg 300
tgggtggttc tgattaattt gaaatgataa ggaattcttt tgttctcttt ttctttttgt 360
ttttataaac ttgttatcgt tctgtatgct aggtatatgg cttatataat ggatacaaat 420
gaagaagaga gggcgaaggt atttcctgat tttttattta tgtttcttag tgtgctataa 480
atgtgtcaga gatgataaga rttcacgtgt ctgtagaatt ttcagcaatg tttatggttg 540
aatttaactt agctaactgt tatgacttac ttgctacatt gaacagggca aaacagttga 600
agttggaagg gctcattttg aaactgcgag tacgagattt accattttgg atgctccggt 660
aagagaccaa cttaaaagaa taattttttg ttcacttgtc tttatgagag tattttgttc 720
taattctttt gcgccttttt tgaacttgta gggtcacaag agttatgtac caaatatgat 780
tagtggagca tctcaagcgg acattggtgt actggtaagt tattatctta atttggtcgg 840
agtcgttact gtgtagtgtg cgtctttggt aggaagctta ttaattttca tgtccttgtc 900
cctctgttgt aggtgatttc ggctcgtaaa ggtgaatttg aaacgggata tgagaggggt 960
gggcagaccc gtgaacatgt tcaacttgca aaaacattgg g 1001
<210> 74
<211> 1001
<212> DNA
<213> Brassica napus
<400> 74
tcagctctac tttcataata gatatatcat ctgcacttgt ttagactcag ggcttaaaag 60
cgtatgtaac acattcttga aattaggatc atgagctttt agtcggtgtg ttttaacttt 120
taagcttctt gatttaatct ttacatggtc accttttcaa ttgtagatgc tggaaagtct 180
acaattggag gacaaattct cttccttagc ggtcaggtgg acgaccgaca aatccaaaag 240
tatgaaaaag aagcaaaaga aaaaagtaga gaaagctggt gggtggttct gattaatttg 300
aaatgataag gaattctttt gttctctttt tctttttgtt tttataaact tgttatcgtt 360
ctgtatgcta ggtatatggc ttatataatg gatacaaatg aagaagagag ggcgaaggta 420
tttcctgatt ttttatttat gtttcttagt gtgctataaa tgtgtcagag atgataagaa 480
ttcacgtgtc tgtagaattt ycagcaatgt ttatggttga atttaactta gctaactgtt 540
atgacttact tgctacattg aacagggcaa aacagttgaa gttggaaggg ctcattttga 600
aactgcgagt acgagattta ccattttgga tgctccggta agagaccaac ttaaaagaat 660
aattttttgt tcacttgtct ttatgagagt attttgttct aattcttttg cgcctttttt 720
gaacttgtag ggtcacaaga gttatgtacc aaatatgatt agtggagcat ctcaagcgga 780
cattggtgta ctggtaagtt attatcttaa tttggtcgga gtcgttactg tgtagtgtgc 840
gtctttggta ggaagcttat taattttcat gtccttgtcc ctctgttgta ggtgatttcg 900
gctcgtaaag gtgaatttga aacgggatat gagaggggtg ggcagacccg tgaacatgtt 960
caacttgcaa aaacattggg cgtgtcgaag ctggttgtcg t 1001
<210> 75
<211> 1001
<212> DNA
<213> Brassica napus
<400> 75
gctctacttt cataatagat atatcatctg cacttgttta gactcagggc ttaaaagcgt 60
atgtaacaca ttcttgaaat taggatcatg agcttttagt cggtgtgttt taacttttaa 120
gcttcttgat ttaatcttta catggtcacc ttttcaattg tagatgctgg aaagtctaca 180
attggaggac aaattctctt ccttagcggt caggtggacg accgacaaat ccaaaagtat 240
gaaaaagaag caaaagaaaa aagtagagaa agctggtggg tggttctgat taatttgaaa 300
tgataaggaa ttcttttgtt ctctttttct ttttgttttt ataaacttgt tatcgttctg 360
tatgctaggt atatggctta tataatggat acaaatgaag aagagagggc gaaggtattt 420
cctgattttt tatttatgtt tcttagtgtg ctataaatgt gtcagagatg ataagaattc 480
acgtgtctgt agaattttca rcaatgttta tggttgaatt taacttagct aactgttatg 540
acttacttgc tacattgaac agggcaaaac agttgaagtt ggaagggctc attttgaaac 600
tgcgagtacg agatttacca ttttggatgc tccggtaaga gaccaactta aaagaataat 660
tttttgttca cttgtcttta tgagagtatt ttgttctaat tcttttgcgc cttttttgaa 720
cttgtagggt cacaagagtt atgtaccaaa tatgattagt ggagcatctc aagcggacat 780
tggtgtactg gtaagttatt atcttaattt ggtcggagtc gttactgtgt agtgtgcgtc 840
tttggtagga agcttattaa ttttcatgtc cttgtccctc tgttgtaggt gatttcggct 900
cgtaaaggtg aatttgaaac gggatatgag aggggtgggc agacccgtga acatgttcaa 960
cttgcaaaaa cattgggcgt gtcgaagctg gttgtcgttg t 1001
<210> 76
<211> 1001
<212> DNA
<213> Brassica napus
<400> 76
ggtcaccttt tcaattgtag atgctggaaa gtctacaatt ggaggacaaa ttctcttcct 60
tagcggtcag gtggacgacc gacaaatcca aaagtatgaa aaagaagcaa aagaaaaaag 120
tagagaaagc tggtgggtgg ttctgattaa tttgaaatga taaggaattc ttttgttctc 180
tttttctttt tgtttttata aacttgttat cgttctgtat gctaggtata tggcttatat 240
aatggataca aatgaagaag agagggcgaa ggtatttcct gattttttat ttatgtttct 300
tagtgtgcta taaatgtgtc agagatgata agaattcacg tgtctgtaga attttcagca 360
atgtttatgg ttgaatttaa cttagctaac tgttatgact tacttgctac attgaacagg 420
gcaaaacagt tgaagttgga agggctcatt ttgaaactgc gagtacgaga tttaccattt 480
tggatgctcc ggtaagagac maacttaaaa gaataatttt ttgttcactt gtctttatga 540
gagtattttg ttctaattct tttgcgcctt ttttgaactt gtagggtcac aagagttatg 600
taccaaatat gattagtgga gcatctcaag cggacattgg tgtactggta agttattatc 660
ttaatttggt cggagtcgtt actgtgtagt gtgcgtcttt ggtaggaagc ttattaattt 720
tcatgtcctt gtccctctgt tgtaggtgat ttcggctcgt aaaggtgaat ttgaaacggg 780
atatgagagg ggtgggcaga cccgtgaaca tgttcaactt gcaaaaacat tgggcgtgtc 840
gaagctggtt gtcgttgtga acaaaatgga tgatccaact gtgaactggt cgaaagagag 900
gtatgtgcat tatcctttcg aattcatgct actgtttttc gtatctactt ccattcatcc 960
gcgtatgtac tcttgtgcag gtacgatgaa atagaacaaa a 1001
<210> 77
<211> 1001
<212> DNA
<213> Brassica napus
<400> 77
tcaattgtag atgctggaaa gtctacaatt ggaggacaaa ttctcttcct tagcggtcag 60
gtggacgacc gacaaatcca aaagtatgaa aaagaagcaa aagaaaaaag tagagaaagc 120
tggtgggtgg ttctgattaa tttgaaatga taaggaattc ttttgttctc tttttctttt 180
tgtttttata aacttgttat cgttctgtat gctaggtata tggcttatat aatggataca 240
aatgaagaag agagggcgaa ggtatttcct gattttttat ttatgtttct tagtgtgcta 300
taaatgtgtc agagatgata agaattcacg tgtctgtaga attttcagca atgtttatgg 360
ttgaatttaa cttagctaac tgttatgact tacttgctac attgaacagg gcaaaacagt 420
tgaagttgga agggctcatt ttgaaactgc gagtacgaga tttaccattt tggatgctcc 480
ggtaagagac caacttaaaa saataatttt ttgttcactt gtctttatga gagtattttg 540
ttctaattct tttgcgcctt ttttgaactt gtagggtcac aagagttatg taccaaatat 600
gattagtgga gcatctcaag cggacattgg tgtactggta agttattatc ttaatttggt 660
cggagtcgtt actgtgtagt gtgcgtcttt ggtaggaagc ttattaattt tcatgtcctt 720
gtccctctgt tgtaggtgat ttcggctcgt aaaggtgaat ttgaaacggg atatgagagg 780
ggtgggcaga cccgtgaaca tgttcaactt gcaaaaacat tgggcgtgtc gaagctggtt 840
gtcgttgtga acaaaatgga tgatccaact gtgaactggt cgaaagagag gtatgtgcat 900
tatcctttcg aattcatgct actgtttttc gtatctactt ccattcatcc gcgtatgtac 960
tcttgtgcag gtacgatgaa atagaacaaa aaatggtacc a 1001
<210> 78
<211> 1001
<212> DNA
<213> Brassica napus
<400> 78
aaagtctaca attggaggac aaattctctt ccttagcggt caggtggacg accgacaaat 60
ccaaaagtat gaaaaagaag caaaagaaaa aagtagagaa agctggtggg tggttctgat 120
taatttgaaa tgataaggaa ttcttttgtt ctctttttct ttttgttttt ataaacttgt 180
tatcgttctg tatgctaggt atatggctta tataatggat acaaatgaag aagagagggc 240
gaaggtattt cctgattttt tatttatgtt tcttagtgtg ctataaatgt gtcagagatg 300
ataagaattc acgtgtctgt agaattttca gcaatgttta tggttgaatt taacttagct 360
aactgttatg acttacttgc tacattgaac agggcaaaac agttgaagtt ggaagggctc 420
attttgaaac tgcgagtacg agatttacca ttttggatgc tccggtaaga gaccaactta 480
aaagaataat tttttgttca yttgtcttta tgagagtatt ttgttctaat tcttttgcgc 540
cttttttgaa cttgtagggt cacaagagtt atgtaccaaa tatgattagt ggagcatctc 600
aagcggacat tggtgtactg gtaagttatt atcttaattt ggtcggagtc gttactgtgt 660
agtgtgcgtc tttggtagga agcttattaa ttttcatgtc cttgtccctc tgttgtaggt 720
gatttcggct cgtaaaggtg aatttgaaac gggatatgag aggggtgggc agacccgtga 780
acatgttcaa cttgcaaaaa cattgggcgt gtcgaagctg gttgtcgttg tgaacaaaat 840
ggatgatcca actgtgaact ggtcgaaaga gaggtatgtg cattatcctt tcgaattcat 900
gctactgttt ttcgtatcta cttccattca tccgcgtatg tactcttgtg caggtacgat 960
gaaatagaac aaaaaatggt accatttctt aaatcctctg g 1001
<210> 79
<211> 1001
<212> DNA
<213> Brassica napus
<400> 79
ctcttcctta gcggtcaggt ggacgaccga caaatccaaa agtatgaaaa agaagcaaaa 60
gaaaaaagta gagaaagctg gtgggtggtt ctgattaatt tgaaatgata aggaattctt 120
ttgttctctt tttctttttg tttttataaa cttgttatcg ttctgtatgc taggtatatg 180
gcttatataa tggatacaaa tgaagaagag agggcgaagg tatttcctga ttttttattt 240
atgtttctta gtgtgctata aatgtgtcag agatgataag aattcacgtg tctgtagaat 300
tttcagcaat gtttatggtt gaatttaact tagctaactg ttatgactta cttgctacat 360
tgaacagggc aaaacagttg aagttggaag ggctcatttt gaaactgcga gtacgagatt 420
taccattttg gatgctccgg taagagacca acttaaaaga ataatttttt gttcacttgt 480
ctttatgaga gtattttgtt ytaattcttt tgcgcctttt ttgaacttgt agggtcacaa 540
gagttatgta ccaaatatga ttagtggagc atctcaagcg gacattggtg tactggtaag 600
ttattatctt aatttggtcg gagtcgttac tgtgtagtgt gcgtctttgg taggaagctt 660
attaattttc atgtccttgt ccctctgttg taggtgattt cggctcgtaa aggtgaattt 720
gaaacgggat atgagagggg tgggcagacc cgtgaacatg ttcaacttgc aaaaacattg 780
ggcgtgtcga agctggttgt cgttgtgaac aaaatggatg atccaactgt gaactggtcg 840
aaagagaggt atgtgcatta tcctttcgaa ttcatgctac tgtttttcgt atctacttcc 900
attcatccgc gtatgtactc ttgtgcaggt acgatgaaat agaacaaaaa atggtaccat 960
ttcttaaatc ctctggctac aacacaaaga aaggtatgca g 1001
<210> 80
<211> 760
<212> DNA
<213> Brassica napus
<400> 80
aattcttttg ttctcttttt ctttttgttt ttataaactt gttatcgtct tgtatgctag 60
gtatatggct tatataatgg atacaaatga agaagagagg gcgaaggtat ttcctgattt 120
ttttatttat gtttctttgt gtgctataaa tgtgtcagag atgataagag ttcacgtgtc 180
tgtagaattt tcagcaatgt ttatggttga atttaactta gctaactgtt atgacttact 240
tgctacattg aacagggcaa aacagttgaa gttggaaggg ctcattttga aactgcgagt 300
acgagattta ccattttgga tgctccggta agagacmaac ttaaaasaat aattttttgt 360
tcacttgtct ttatgagagt attttgttct aattcttttg cgcctttttt gaacttgtag 420
ggtcacaaga gttatgtacc aaatatgatt agtggagcat ctcaagcgga cattggtgta 480
ctggtaagtt attatcttaa tttggtcgga gtcgttactg tgtagtgtgc gtctttggta 540
ggaagcttat taattttcat gtccttgtcc ctctgttgta ggtgatttcg gctcgtaaag 600
gtgaatttga aacgggatat gagaggggtg ggcagacccg tgaacatgtt caacttgcaa 660
aaacattggg cgtgtcgaag ctggttgtcg ttgtgaacaa aatggatgat ccaactgtga 720
actggtcgaa agagaggtat gtgcattatc ctttcgaatt 760
<210> 81
<211> 201
<212> DNA
<213> Brassica napus
<400> 81
ccagakgcaa aacygaatgt aagagaacat tactattaga aatcgagatc aagcttcctc 60
ttcagatgag atctatcggc actacaaaat gaacaacaac ragaagcttt taaaacacat 120
wcaakcttga gmctgtaaaa acaactaatc aaagagatcg ctctatttgc wcmtggtgat 180
ggttggtcgg kkaggaggcc g 201
<210> 82
<211> 121
<212> DNA
<213> Brassica napus
<400> 82
yagaaactgg tcgatgaggt aaccatgtgc accgtgaacc tccaccccat caaagcctat 60
rawtamcaca agtgtcatgt tcagctaaca tyatacatcc aaaggggaaa aagacttttc 120
t 121
<210> 83
<211> 121
<212> DNA
<213> Brassica napus
<400> 83
ratgcatttt cttcatcatt ggtaacatgg tgctttatgt yaacaataaa acctcttaat 60
ragctctaac tgattcgtaa tgaaaccaaa catatataaa taaacaatct tagatttgat 120
g 121
<210> 84
<211> 121
<212> DNA
<213> Brassica napus
<400> 84
gtcttttatc ttatttcaaa atatattagg tgtgacatgt gaaaaggttc tagaaactgg 60
ktggttatgc ttcctagact ccatcaagaa ataaagctga attgtttttt tacmcatcca 120
c 121
<210> 85
<211> 121
<212> DNA
<213> Brassica napus
<400> 85
aaactggktg gttatgcttc ctagactcca tcaagaaata aagctgaatt gtttttttac 60
mcatccactc atttttkatc aaacaggtac aagagaagaa gatcaagaaa atttctgaaa 120
t 121
<210> 86
<211> 121
<212> DNA
<213> Brassica napus
<400> 86
yactgcgata tcatctccta ttatacycca tgtttctttg aagaactctg ysgtataacc 60
rtctggccct ggagctttgc ttcctggcat cttaaataaa actcctttga tttcttcttt 120
r 121
<210> 87
<211> 121
<212> DNA
<213> Brassica napus
<400> 87
attattagtt tttccaaaat caaatgttat tagattgtac atataaaaaa aaacctaaga 60
raaaamaact catctcattc ttttatacta agakggtcta aagaaattaa tgatataaaa 120
a 121
<210> 88
<211> 1001
<212> DNA
<213> Brassica napus
<400> 88
ctatgtgtag tagataaagt atggtactga ccataggtat gtgacatttt agtttatatt 60
gaatgttaat atttgatatt gtatgctaac tataaacaca tgtgttattt taatagttga 120
accacaacca aagtcagcct ctatacatga ttacatccag ctggagatta catgacacat 180
gcaccagagg catacacact tggatctcta atcctaacac ctccaattac aggccaaaga 240
agacaaggat gttgttaaaa ggtgaattca aggtagacct aagctaactt cagctgatac 300
tgaacttgca ctgatgtgat tgttattaat atcattaatc gttattgaaa gttctcaaca 360
tcaaattgat tgttcatgaa acaaggtgtc atagggtgaa gagaaagaac ctgtaggaag 420
tgtggtgaaa ctgatcacag tcgcgctctc tgcaaaataa tgtataacac tattacttag 480
cggttacaca ttagttttga rccttgaagt tcacttgaaa acacgtagga gagaagcaaa 540
tagaacagcc actagcgtga tgcagatagg aatgcctata aacatgtgtg cagttgtatt 600
cttgagaagg tggatgatat ggcagatcct tgtggcttga tgattcagta cataaaaggg 660
catatcagtt tctttgaaaa aggttctttg ttgtacctat aaaagaaagg agattgtttc 720
aaaaaagaac tacataaatc atgctagaga ctggatccag agtataaata ttcacctctt 780
cgtttggttt gtccctaaaa tgttctacag aatcagcttc ttcaagggaa tcacatccgt 840
gtgcatcaag aaactgtaga ctcagtggga gtttttccac tgatctgaga ttcttgcagt 900
tattaaggca aagagttacc agtgaggtaa gatctctgat gcttgatggc aatgcaacaa 960
actcatggcc gctgagatct aaatttgtca acttaatgaa a 1001
<210> 89
<211> 201
<212> DNA
<213> Brassica napus
<400> 89
gagaaagaac ctgtaggaag tgtggtgaaa ctgatcacag tcgcgctctc tgcaaaataa 60
tgtataacac tattacttag cggttacaca ttagttttga rccttgaagt tcacttgaaa 120
acacgtagra gagaagcaam tagaacagcs actagcgtga tgcagatagg aatgcctata 180
aacatgwgtg cagttgtatt c 201
<210> 90
<211> 301
<212> DNA
<213> Brassica napus
<400> 90
aatggaactt cagttgataa tggagtwtat gaaggargga acacttgaag tcttccctgt 60
cttctatgga gttgatcctt ccaccgtcag gcatcagcta gggagtttct ctttagaacg 120
gtacaagggt cgtccagaaa tggtgcacaa rgttcacaag tggagagaag ctcttcacct 180
aatagctaac ctttcaggcc tggattcaag acaytggtaa gctcttctta ccataatata 240
gttacaaaaa tatatatact tacgtctttt ctttaaaaat atattttcta gtgttttcac 300
a 301
<210> 91
<211> 229
<212> DNA
<213> Brassica napus
<400> 91
tttaaattta gtgactaaac cagaatgaga caggtgaaac tcctagcgag atactattac 60
cttacgcgct ttgattagtt tgttttgagt ctctagcttt gcacgttgct ccctcctcct 120
cataattgca tggagctgct tcrcattgac aaagacaggc tcatcttcaa tgaggtccag 180
tggtaaagga acccgaccca ccatttgggg attccatacc tgcaggtct 229
<210> 92
<211> 229
<212> DNA
<213> Brassica napus
<400> 92
tttaaattta gtgactaaac cagaatgaga caggtgaaac tcctagckag atactattac 60
cttacgcgct ttgattagtt tgttttgagt ctctagcttt gcacgttgct ccctcctcct 120
cataattgca tggagctgct tcgcattgac aaagacaggc tcatcttcaa tgaggtccag 180
tggtaaagga acccgaccca ccatttgggg attccatacc tgcaggtct 229
<210> 93
<211> 121
<212> DNA
<213> Brassica napus
<400> 93
aaccaaaaga gtgtgcggtt tgatactcaa agtagtagtg atcttgaaac tgtggtgaga 60
ycttttggta aacacawctc gcaagtgttg ttttgccaac tytttcctca ccacaaatgc 120
c 121
<210> 94
<211> 121
<212> DNA
<213> Brassica napus
<400> 94
ctcaaatatc tgaaaatyta caaccacagg cgttacaaaa gtttagattc waggacacag 60
rgcaatccaa acgaaatttt acaaccttat aaacttagat tgctacagtg ggatgcatat 120
c 121
<210> 95
<211> 970
<212> DNA
<213> Brassica napus
<400> 95
aaccggagat gaccatcatt acctagctat tgcccatcgt cttacataga agaagcacac 60
ccaaacatag agcaaaagcc attagtttca aggtttcgga gctaaaacgg tctgtgtaga 120
ttgttctgac actcctcatc cctcgatcaa gcgtattcaa tatcttaggt ttctggatgc 180
aagcacatag ctgtacctat taaacgaaat aaaaggaagt tagctttgcg tatagagatt 240
gagagaataa ggattacagt agagggttgt tttacctctt cactacgtct tcctgatgga 300
aaacgagtga attgacttaa aaactgattc ggttggaagc aagggctgag atcaaggtca 360
tcaacagaat gatcaactga aagggagaaa gtttctaagg acatgcagcc atgtgaatat 420
aggtgcttaa tgctcaatgg aagctctgaa agtgatttta gtttcatgca atagttgagg 480
cagagagtta tcagcgagga kaggtctttg atgcttgtag gcactgtttc aaaatcgtgg 540
cgactgatat ctaaatacgt caactttgta aaaaaccgaa gctgatccga caatgtctcg 600
acatgtttgc agttgtcaag acgaagctca agcaaattgt attttccatg gtcttgttct 660
gcctgcgaaa tgctcaccaa tgtgtggagg ttggtacaat cagaaagtgt gagcgtctcc 720
agctgatata gttgtggcaa agcttcaagt ctacgacagt tacaaagcct cacatgtttt 780
aacttcgtaa gatgagtcat tgatgaaggt aaacccctga agaagtttcc acttaggttc 840
aacttctcta gaacttgcat gtgatgaatg tcatctggta tttcttcgat gtttaagttg 900
attagattta gctccatcaa ccagggaaaa tatgaaaagc tataacactc gaaaggatcc 960
ctttgctcac 970
<210> 96
<211> 201
<212> DNA
<213> Brassica napus
<400> 96
gataaaatgg tttgattgat aagtaacctt gcaaatgtag gaaacatatt aagaacagaa 60
agcttcaact tcaattctat tgagagaaag aaaaaagcca yaaagaagca aatactgyac 120
ctctcctgca taatgcgata tcgtaaaatc agtctgagag agcttcggtt tggaaaatct 180
ctcgtgacct ttgaacgtct g 201
<210> 97
<211> 301
<212> DNA
<213> Brassica napus
<400> 97
acatgtgtat gataaatcaa ttgcaaaaca agaaaaatat attatttgtc catcaagaaa 60
ctgatacctt gatgactaaa ttttgtagct ttggggtctt tctgagcaga cgtggcagaa 120
cttgccagct tttatccttg tcgctctcga rtgataaagt aacgaggttg tcgaatgtga 180
agatcaattt gcagccgaaa tgaagcgcct aatgcatgta ttatcgaaac cataagcata 240
tatacagata tatatattca taatagattt atgaattaat tttattttag ttttactatt 300
t 301
<210> 98
<211> 121
<212> DNA
<213> Brassica napus
<400> 98
acaataaatt tataattgtt aaccragtgg tcttggccta gtggtaaatg agttctacct 60
rgagtttctg ccctaggttc gattcccgga ttaagtggca agaaaaaccg gatttatatg 120
g 121
<210> 99
<211> 698
<212> DNA
<213> Brassica napus
<400> 99
agctttcaca aatctccacc agcttggttt aagcccaacc ttggcccatc cgacaaccct 60
agtgccgaca gcttcctccc ttgtctcttt gtctttcttg tgaaatgaga aagtcgatct 120
tgactctctc tctctcttcc tcgtctctct cactcgcgac ccttctccgc cgattcggac 180
ttggtgtcgt cgtgaggagt tataggttgc tctgcctcgc aaccgtagct ccatgaaggt 240
ctcgtcagct ccaccgcgat ggcgtcgcat ctccttctct ccattcttcc ctcgaagtyt 300
cttcgccgaa cggcgatttg cttctctgat gatggctcac accatcgtga ctccatcatc 360
tttcctccct aatatcccca ggtacatcta atctctcctc ttcatgagcg attaggatca 420
tctgttaggg atactaagtg agtcttgtgg gaacttgagc taacttgagg attctttgcc 480
tttgcatgtc gtctcaggtg actcgtgact ctatctctag tcgcgattga gaacctgaca 540
tgtcggtaag ctcttgcctg agctccatca tcacgctcta cctctttcgt agataacgta 600
agctcatgtc ttaacgaacc ttctctaata tgcttttcac ttgtcgatct gactcttctg 660
tttctgtttc agaagccggt ttagctctgt gacgagct 698
<210> 100
<211> 749
<212> DNA
<213> Brassica napus
<400> 100
agcttctggg cagaggcagc atctactgct atccatgtga tcaatagatc acctaactct 60
acactcgagt tcagaatgcc tgaagaattg tggaccggcg cgaaacctga cttgggacat 120
ctaagaactt ttggatgcac tgcgtatgtc cacataactg aggagaaaac aggacctaga 180
gccatcaaag gggtgtttgt ggggtaccct atgggtacta aaggctatcg agtttggata 240
gaggatgarg gcagatgcag aacaagtaga aatgttgttt tcaacgaaga cgagctatac 300
aagcacaccg ttgctaaagc aaaagaaagc acaggagtga ctaaagatac agagaaacga 360
gctaaaaaga gggtctcatt cagtgatgat ttgatcagag ggccttctcc ttctgtcgaa 420
tccaaagaca catctgatca aggtggagaa gaatcatcat catctgagga gtcgagtagt 480
tcgagtgacc aagaacagaa tgacgaggtt gaaagtgaaa gtggtgacac tggatcttta 540
gacacctatg ttctagctcg agacagagca agaagacaga acgtgaggcc cccatctcgc 600
tatgaggatg gaaactttgt agcctatgcg ctaaatgtca tcaacgactt agaggttgaa 660
gaacctaaat cctacgctga ggcaatgaaa agtccacaga agaagttgtg gaaaaatgca 720
gctgaggagg agatggaatc tcataggaa 749
<210> 101
<211> 574
<212> DNA
<213> Brassica napus
<400> 101
aattcttgaa gctagttgca gatttaaaca atttgcaagt tgaagtatcc gaggaagttc 60
aagctatcct attgctaagt tctctaccaa acaagtatga tcaactcaaa gagactctca 120
agtatggaag agacacccta agcctagctg aggttacagg ggctgcaagg tcgaaagaaa 180
gagagttgat tgagagtggt aagtttacca ggtctggtgg agaaggtctg atggtgacag 240
acagaggaag atcagaccaa cgctctggca aaggaaatgg aaaatcctac aggggaagat 300
ccaagagcag acagggacgt tccaagtcgc gtcctaggaa caccaaaggc tcaaagggat 360
gttttgtatg cgggaaggag ggccactgga agcgtgactg ccctgataag aaaccttaca 420
aaacgccaga ctcagcaaat gttgtggcag agtccaakga acctctaatc ctcaccgtga 480
gcacccaata ctccaaggac gaatgggtga tggactctgg ctgctcgttc cacatcacac 540
cagacaagag ttttttgttt gacctggaag aatt 574
<210> 102
<211> 524
<212> DNA
<213> Brassica napus
<400> 102
agcttctcat cggtgagaat agctttcagt ccaagcactc caaagtgagc aagcattctc 60
actttccata gggcgaagtc accatctcct tcgaaacggt cgatctcgat gcgttccttt 120
gacttcaccw ttgagaaggt actgagtatg cacctcctct gtctctctct ctatcctaag 180
caaccttgat gaaaaccgga gctctgatac cacttgtaga atgtaattag ctcaggttaa 240
cttaggttag aggttatatt gatctaggtc taatactgaa agtaaagaca caagcgattt 300
aacgacttcc cggccctcgg cgcggtacgt gtcgtgggag aacttctgct cccaaaatcc 360
actagatcaa agagtctcta gcaccactaa atcagtgtgc tagataggta ggttacaata 420
agatcccttc aacttagcta gggaatacaa caaccttaat atgagacaat agccttaagt 480
ctaagctagt tgtccttgtt gaagtctcct ttcccttgat gctg 524
<210> 103
<211> 681
<212> DNA
<213> Brassica napus
<400> 103
aaagtgagca agcattctca ctttccatag ggcgaagtca ccatctcctt cgaaacggtc 60
gatctcgatg cgttcctttg acttcaccat tgagaaggta ctgagtatgc acctcctctg 120
tctctctctc tatcctaagc aaccttgatg aaaaccggaa gctctgatac cacttgtaga 180
atgtaattag ctcaggttaa cttaggttag aggttatatt gatctaggtc taatactgaa 240
agtaaagaca caagcgattt aacgacttcc cggccctcgg ctcggtacgt gtcgtgggag 300
aacttctgct cccaaaatcy actagatyaa agagtctcta gcaccactaa atcagtgtgc 360
tagataggta ggttacaata agatcccttc aacttagcta gggaatacaa caaccttaat 420
atgagacaat agccttaagt ctaagctagt tgtccttgtt gaagtctcct ttcccttgat 480
gctgtatctt gttgactgat ctctgatgct ataacctgct gttgttgctt ctatccggta 540
accctaatca cccatactaa cattgtatat atatgtgtcg tatgtgatca ggtagtgcaa 600
cctggagtgg gcctgcgcat gacttcggcc catcagatgt gatgctgctg ctggcccatc 660
acgcagggat aaacccaagc t 681
<210> 104
<211> 519
<212> DNA
<213> Brassica napus
<400> 104
gctgcaatcg ccttttgtgg gaggttgatg caagggaata gatacatagc tccttcagct 60
ctgttgcatg ttacaccktc taacttgttc aaagcttctt caagggtctg tgtggctcaa 120
ctcaaaactc attaaaacca taacaaaaat ctagttccat gattgctagt aagttctgtg 180
agaatggcta acctttgcac gtgttgctaa agacgagagg atcccctctt tctctgctat 240
gtatgaatca tacgagtcat caccaggctg atcaaattac attaaagagc tgttaaagac 300
aattgagctt ttaaaccaaa ccagctgtta aagtaataca ataccttggg agggctcatg 360
acgaggctgg cgagaatttg accagagatg ttggagcaaa gattgacaga agccactttg 420
tatatctgtt ctcttacatc agaagtgaat ccggtaacct ccatgtaacc gcctctcttc 480
ccacactctc cataataccc tattgaaaga aaggagtac 519
<210> 105
<211> 946
<212> DNA
<213> Brassica napus
<400> 105
tatatgttct gaacctcact acaattcagc atctgagctt gttttttttt ctttcttcta 60
gtggtgtaag attttgttta accaatggat gttgcaattt ttctatcagt tcggattcta 120
ttgatcgtgc ttggaagatc ctcgaccaaa tccccgggaa agctaccggt gcttacagcc 180
acagccaggt tttgtggcct ttgtcaatct taaacagtga atgatggatg atacactctc 240
ttaatcttct gctttgtctc tcagggtatc aagggactac gtgatgcgat agctgctgga 300
atcgaagccc gtgacggttt ccctgctgat cctaatgata ttttcatgac agatggtgca 360
agccctgggg taaccagtca tcaaactttc cctaaactta tataaattac agaaaaaagg 420
ttagtaatgt tactctcgtt cttttaggtt cacatgatga tgcaactcct cataagttca 480
gagaaagatg gaatcctttg ycctattcct cagtacccct tgtactcagc ttctattgcc 540
cttcacggcg gtagtctggt atgttccttt atgtctctct gatgcatgtc tatagtgact 600
tctgattgct gtcatcttct tgaggtaggt tccatactac ctagacgaag catcagggtg 660
gggtcttgaa atatctgagc tgaagaagca gcttgaggat gctaagtcaa aaggcatcac 720
tgtaagagcc ttggcggtta ttaaccctgg taaccctaca ggacaggtaa agactaaacc 780
acaaatctat ttccatccaa attcaacact ttgtctgaac tctagcctgt tattttcctg 840
gttaaaggtt ctgtcagaag aaaaccagcg tgacattgtt gatttctgta agaaagaagg 900
cttggtgctt ctagcagacg aggtttatca ggaaacgttt acgtcc 946
<210> 106
<211> 121
<212> DNA
<213> Brassica napus
<400> 106
tctcgtgacc cataatcaag cattgaatat atattctgag agaagccata tatattcaaa 60
ytaattaaaa actaatmtat tcwgaaagta twgtgaaata ttgaatatta cgagcaagta 120
g 121
<210> 107
<211> 121
<212> DNA
<213> Brassica napus
<400> 107
atcaacaaga gaaaagctaa caagacttgc acataaytct aaacaggaag aaaaactgag 60
yagaacatga acagagacca aaaggtccaa aactgatttg atttaagggc cagtttgaat 120
a 121
<210> 108
<211> 408
<212> DNA
<213> Brassica napus
<400> 108
agtttggttt aatgtgatta acaattgtgg tttaggtgta tggtccaacg aggttgatat 60
ggggagcgaa aggagaggag caagaggcgg ggacgaagga gttcattgag atgctcaaga 120
tgctagagtc tgagcttgga gacaagactt actttggagg ygaaacattc ggttatgtgg 180
atatagctat gattggattc tactgctggt tcgacgtttt ggagaagtgt gggaatttta 240
gcatcgaagc agagtgtcca aagctgattg cttgggctaa aaggtgtatg aagagagaga 300
gtgtggctaa gtctcttcct gattcaaaca agatcactaa gttcgttcct gagctaaaga 360
aaaaaattgg catcgagtag ttctgttata atttaatctg tgttctgt 408
<210> 109
<211> 301
<212> DNA
<213> Brassica napus
<400> 109
tgaagcataa cctcaaacgc ctcagcaaca tcttcacagt aaagatagct acgaacatta 60
gtaccatctc tatgaatagg aagagctttc cctcttatca ccaacaaaaa taaacttagg 120
tccaacaaca gcatcttcat ctgtctcagc yatwaacctc atctgctctc acatgtataa 180
mcctcctgat ctatcctcta gtcactttac aagcctcgag aaggacatgc gttccgtaga 240
tattgttttt tagtgaactc gaaactgtta cggaaggagt tgtccacgtg agttctgtga 300
g 301
<210> 110
<211> 301
<212> DNA
<213> Brassica napus
<220>
<221> misc_feature
<222> (242)..(271)
<223> 其中n为a、t、c或g
<400> 110
acaaagctgt aattaaaaga taagacacga gtttcggttt ggttttgtgg ataaaaggat 60
aagaagagca ttttcccctt ttttatcgta atttttgttt tataaaagga taattatatt 120
aagtttattt tgtttataca acgatcaatg ktttttgtaa ttctcattta tataacgatt 180
ctcacacctt tattgtttta gatggatatg ttgggtgaca actcacaagt agattttttc 240
annnnnnnnn nnnnnnnnnn nnnnnnnnnn ngctagttgg taaaaagaaa aagaaaagaa 300
g 301
<210> 111
<211> 1212
<212> DNA
<213> Brassica napus
<220>
<221> misc_feature
<222> (78)..(78)
<223> 其中n为a、t、c或g
<220>
<221> misc_feature
<222> (1113)..(1113)
<223> 其中n为a、t、c或g
<400> 111
cccgcgtccg ctgacggaca cggagatggc attgtgttac tggaaagctc tgttactact 60
actgctttca tgcctctntc agtgtttcgt atgctagtct cggcgatgcc gatccaaact 120
acagggcatg tgttggagaa tgcgagataa gcggctgcgt tggacaacta tgctttcctc 180
agtgcaactc ttcatccaac actggtccat ggtacacaca agagcctctg tacctacaat 240
ggcaaaagtg gggatgtcaa ggtgattgcc gttaccactg tatggttaac agagagaaag 300
aacgcgaaac tctcggtcaa cccccactca agtatcatgg taaatggcct ttcaagcgtc 360
tccttgggat tcaggagcct gcttctgttg ctttctctgt gctcaaccta gcgatgcatt 420
tccacggctg gatctccttc ttcattacgc tttactataa gctgcctctc agagaagata 480
agacggctta ctatgaatac gttggtctgt ggcatatcta cggtttcttg tcaatgaact 540
cttggttctg gagtgcggtt ttccacactc gggatgttga catcactgag aggttggact 600
actcgtctgc aatagcggtt atcggattct cactcattgt atccatcttg agaacgtttg 660
atgttcgggt agaggctgca agagtcatgg tatctgctcc agtgctagct tttgtcacca 720
ctcacatact gtatattaac ttctacaagc tcgactatgg ttggaacatg attgtgtgtg 780
tggccatggg agtcgctcag cttctcctat gggcaagatg ggctgctgtc tctagacatc 840
cttctaastg gaaactttgg atggtggtga tagcttcagg cttagctatg cttttggaga 900
tatatgactt tcctccatat gaaggctact tcgatgctca ctccatttgg catgctgcaa 960
ccattcctct aactgttctc tggtggagct ttattagaga cgatgctgag ttcagaactt 1020
ctagtcttct caagaaatct aagacaaagg ctaagtaagc ttatttgtct gacagatgca 1080
gaggtttctt gagtttttat ttccaatgtt ttntattcag agatttgtct tggccgtcct 1140
tacttttggt caattgagat ttgatattag tttctcattc atacacacgc ttatgctaat 1200
ctttttggac tc 1212
Claims (27)
1.一种鉴定与卡诺拉中的期望营养性状相关的数量性状基因座的方法,所述方法包括:
分析一个群体的卡诺拉植物或种质的期望营养性状;
使用选自SEQ ID NO:1到SEQ ID NO:111的组的至少一个标记来确定所述卡诺拉植物或种质的基因型;
针对与所述标记相关的数量性状基因座(QTL)的存在进行所述卡诺拉植物或种质的作图;和
使所述QTL与所述期望营养性状相关联。
2.根据权利要求1的方法,其中该至少一个标记包括单核苷酸多态性(SNP)。
3.根据权利要求1的方法,其中所述卡诺拉植物或种质包含来自YN01-429系或其谱系的低纤维含量和YSC。
4.权利要求1的标记。
5.包含与QTL相关的序列的分离核酸和/或重组核酸。
其中该QTL与卡诺拉植物或种质中的期望营养性状相关;并且
其中该QTL进一步与选自SEQ ID NO:1到SEQ ID NO:111的组的至少一个标记相关。
6.权利要求5的标记,其中该标记包括SNP。
7.权利要求5的序列,其中该序列在YSC系YN01-429或其谱系中被鉴定。
8.权利要求5的卡诺拉植物或种质。
9.权利要求5的卡诺拉植物或种质,其中所述卡诺拉植物或种质包含来自YSC系YN01-429或其谱系的低纤维含量和YSC性状。
10.一种选择包含期望营养性状的卡诺拉植物或种质的方法,该方法包括:
检测卡诺拉植物或种质中的与QTL连锁的至少一个标记,所述标记选自SEQ ID NO:1到SEQ ID NO:111的组,其中该QTL与卡诺拉植物或种质中的期望营养性状相关;和
基于该标记的存在选择卡诺拉植物或种质。
11.根据权利要求10的方法,进一步包括对来自所述卡诺拉植物或种质的核酸样品测试所述QTL的存在。
12.根据权利要求10的方法,其中所述标记包括SNP的至少一种等位基因形式。
13.根据权利要求10的方法,其中所述卡诺拉植物或种质包含来自YSC系YN01-429的低纤维含量和YSC性状。
14.权利要求10的卡诺拉植物或种质。
15.权利要求10的标记。
16.从权利要求8的植物的种子生产的粕。
17.从权利要求14的植物的种子生产的粕。
18.一种鉴定显示减少的纤维的卡诺拉植物的方法,该方法包括检测卡诺拉植物的种质中的标记基因座的至少一个等位基因,其中:
a.该标记基因座位于包含DBSNP01120和DBSNP02172并且被DBSNP01120和DBSNP02172所侧翼的染色体区间内;并且
b.所述至少一个等位基因与减少的纤维相关。
19.权利要求18的方法,其中该标记基因座位于包含sub-DBSNP01120和sub-DBSNP02172并且被sub-DBSNP01120和sub-DBSNP02172所侧翼的染色体区间内。
20.权利要求18的方法,其中卡诺拉植物属于硬茎(Stiff Stalk)杂种优势群。
21.通过权利要求18的方法鉴定的卡诺拉植物。
22.一种鉴定显示减少的纤维的卡诺拉植物的方法,该方法包括检测卡诺拉植物的种质中在一个或多个标记基因座处包含等位基因的单倍型,其中:
该一个或多个标记基因座位于包含DBSNP01120和DBSNP02172并且被DBSNP01120和DBSNP02172侧翼的染色体区间内;并且,
所述单倍型与减少的纤维相关。
23.权利要求22的方法,其中该一个或多个标记基因座位于包含DBSNP01120和DBSNP02172并且被DBSNP01120和DBSNP02172侧翼的染色体区间内。
24.通过权利要求22的方法鉴定的卡诺拉植物,其中所述卡诺拉植物在其种质内包含与减少的纤维相关的单倍型,其中所述单倍型包含在一个或多个标记基因座处的等位基因,所述标记基因座位于包含DBSNP01120和DBSNP02172并且被DBSNP01120和DBSNP02172侧翼的染色体区间内。
25.一种标记辅助的卡诺拉植物选择方法,包括:
a.获得具有标记基因座的至少一个等位基因的第一卡诺拉植物,其中该标记基因座位于包含DBSNP01120和DBSNP02172并且被DBSNP01120和DBSNP02172所侧翼的染色体区间内,并且该标记基因座的等位基因与减少的纤维相关;
b.使该第一卡诺拉植物与第二卡诺拉植物杂交;
c.评价后代的所述至少一个等位基因;和
d.选择具备所述至少一个等位基因的后代植物。
26.权利要求25的方法,其中该标记基因座位于包含DBSNP357221和DBSNP222231并且被DBSNP357221和DBSNP222231所侧翼的染色体区间内。
27.通过权利要求25的方法选择的卡诺拉后代植物,其中所述植物具有标记基因座的至少一个等位基因,其中该标记基因座位于包含DBSNP01120和DBSNP02172并且被DBSNP01120和DBSNP02172所侧翼的染色体区间内。
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ES2843271T3 (es) | 2014-12-18 | 2021-07-16 | Dow Agrosciences Llc | Cartografiado fino y validación de QTL subyacentes a los rasgos de contenido de fibra y color del recubrimiento de la semilla e identificación de marcadores SNP para selección asistida por marcador de estos rasgos derivados de la línea de colza de recubrimiento de semillas amarillo (YSC) YN01-429 y su linaje |
AU2019402999A1 (en) * | 2018-12-20 | 2021-06-24 | Agrigenetics, Inc. | SNP markers and selection of low fiber in brassica |
WO2020168277A1 (en) * | 2019-02-14 | 2020-08-20 | Cargill, Incorporated | Brassica plants producing elevated levels of polyunsaturated fatty acids |
CN111118203B (zh) * | 2020-02-25 | 2023-04-14 | 贵州省油菜研究所 | 甘蓝型油菜第一次有效分枝数性状的a06染色体主效qtl位点、snp分子标记及应用 |
CN114457184A (zh) * | 2022-02-21 | 2022-05-10 | 广东省农业科学院蔬菜研究所 | 鉴定丝瓜种皮颜色的snp分子标记、kasp引物及其应用 |
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AR103166A1 (es) | 2017-04-19 |
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AU2019200923A1 (en) | 2019-02-28 |
HUE052648T2 (hu) | 2021-05-28 |
AU2015364304B2 (en) | 2018-11-08 |
PL3234197T3 (pl) | 2021-04-19 |
RS61283B1 (sr) | 2021-02-26 |
EP3234197B1 (en) | 2020-11-11 |
EP3234197A4 (en) | 2018-06-13 |
RU2017118904A (ru) | 2019-01-18 |
US20170332593A1 (en) | 2017-11-23 |
EP3831955A1 (en) | 2021-06-09 |
AU2021245186A1 (en) | 2021-11-04 |
WO2016100883A1 (en) | 2016-06-23 |
CA2969547A1 (en) | 2016-06-23 |
AU2015364304A1 (en) | 2017-06-01 |
AU2019200923B2 (en) | 2021-07-08 |
EP3234197A1 (en) | 2017-10-25 |
US20230407417A1 (en) | 2023-12-21 |
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US10791692B2 (en) | 2020-10-06 |
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