CN106927941B - Organic silicon fertilizer and preparation method thereof - Google Patents
Organic silicon fertilizer and preparation method thereof Download PDFInfo
- Publication number
- CN106927941B CN106927941B CN201511030420.5A CN201511030420A CN106927941B CN 106927941 B CN106927941 B CN 106927941B CN 201511030420 A CN201511030420 A CN 201511030420A CN 106927941 B CN106927941 B CN 106927941B
- Authority
- CN
- China
- Prior art keywords
- silicon fertilizer
- fertilizer
- liquid
- parts
- potassium feldspar
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D1/00—Fertilisers containing potassium
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
Abstract
The invention provides an organic silicon fertilizer and a preparation method thereof, wherein the method comprises the following steps: silicate bacteria, aspergillus niger, bacillus licheniformis, bacillus subtilis, lactococcus lactis, lactobacillus plantarum and alcohol yeast are mixed according to the viable count of 30-35: 25-30: 8-10: 8-10: 8-10: 8-10: 13-15, mixing and performing anaerobic fermentation to prepare a zymogen liquid with the pH value of 4.0-3.5; and mixing the sterilized potash feldspar with the zymocyte liquid, selectively adding benzylaminopurine, and fermenting for more than 20 days at the temperature of 28-37 ℃ in a closed and static manner to obtain the product, namely the organic silicon fertilizer. According to the invention, potassium feldspar is used as a raw material, and a microbial enzymolysis technology is utilized to prepare a biological organic silicon fertilizer series product, so that the obtained silicon fertilizer product has good stability, high crop absorption rate and strong practicability.
Description
Technical Field
The invention relates to an organic silicon fertilizer and a preparation method thereof, in particular to a method for preparing the organic silicon fertilizer by a biological enzymolysis technology and the prepared organic silicon fertilizer, belonging to the technical field of novel biological organic fertilizers.
Background
The concept of silicon as a fertilizer is proposed later than nitrogen, phosphorus and potassium. In 1926, the silicon element is firstly found in the United states to increase the yield of rice and sugarcane; professor Taitian Daxiong and Xiaolin of Japan sorb university in 1951 finds and proposes that the rice is related to silicon-deficient elements such as autumn fall, rice blast, premature senility, root rot and the like. The first new fertilizer produced by using industrial waste residue in the world, silicon fertilizer, was developed in 1953, and silicon fertilizer was written into fertilizer method in japan in 1955. Subsequently, the production technology of silicon fertilizer in japan was introduced successively in korea, philippines, thailand, taiwan and the like, so that the silicon fertilizer was widely popularized and applied in the 70 s. The technical experience of Japan is absorbed by America, Mauriques and the like, and the yield of sugarcane is almost doubled. The silicon fertilizer is confirmed to be secondary nitrogen (N) and phosphorus (P) by the international soil academy2O5) Potassium (K)2O) followed by a fourth large element fertilizer. Many of the results of the research in the 13 th international plant nutrition society of 9 th 1997, japan, in the united states, the days, and the middle, showed the possibility that silicin becomes an essential nutrient for plants in the 21 st century. But close toIn Japan, silicon and potassium fertilizers of fly ash are developed in the years, which not only solves the problem of fly ash, but also solves the problem of loss of potassium fertilizers. In the autumn of 1999, the first academic seminar "silicon in agriculture" in the world was held in Florida in the United states, indicating that silicon nutrition has attracted worldwide attention, and also indicating that the climax of international popularization and application of silicon fertilizers has come. China also formally lists silicon fertilizer as one of agricultural fertilizers and promulgates the trial standard of silicon fertilizer industry in 2004.
Silicon has a significant impact on the yield and quality of a variety of crops. The research shows that: the silicon element is a typical beneficial element for rice, and the silicon content in rice plants exceeds the sum of elements such as nitrogen, phosphorus, potassium and the like. In the rice production process, a large amount of silicon is absorbed, 20-50% of silicon is provided by irrigation water, and 75-80% of silicon comes from soil. The yield of rice is 500kg per mu, and the stems and the rice absorb Silicon (SiO)2) The amount of the medicine is as much as 75 kg/mu, which is N, P absorbed in proportion2O5、K2The sum of the three components O is 1.5 times higher. Silicon is a major component of the plant body. The silicon fertilizer can improve the photosynthesis efficiency of rice, improve the activity of root systems, increase the strength of surface tissues, make stalks hard, resist lodging and reduce plant diseases and insect pests. 100-170 million tons of silicon fertilizer (slag calcium silicate) is produced by using smelting waste slag every year in Japan and Korea, and is mainly used for rice.
Besides rice and sugarcane, the silicon fertilizer has yield increasing effects of different degrees on crops such as wheat, corn, fruits, vegetables, peanuts, cotton, yellow tobacco and the like. 175 plants were studied in Hippocastana, wherein: there are 42 silaphilic plants, accounting for 24%. For plants with excessive silica, such as Gramineae and Cyperaceae, silica is an essential element, for plants with excessive silica, such as tomato and cucumber, the silica is a beneficial element.
At present, silicon fertilizers in the market are various, for example, silicon nitrogen phosphorus compound fertilizers researched by Liaoning agricultural academy of sciences, which are prepared by taking industrial waste residues, fly ash and nitrogen phosphorus fertilizers as raw materials; SiO produced by Baixing superfine mill in Tonghua City2Calcium fertilizer with CaO not less than 36.0%, CaO not less than 20.0%, MgO not less than 6.0%, and potassium oxide not less than 0.6%. In other reported smeltersThe slag can also be used as silicon fertilizer. CN1962564A discloses a process for producing an organic silicon fertilizer, wherein, three substances of silicon dioxide, aluminum oxide and calcium oxide are used as raw materials to react with water, and the reactant is dried, crushed and mixed with livestock and poultry manure for fermentation to prepare the organic silicon fertilizer. CN101096326A discloses an organic silicon foliar fertilizer and a preparation method thereof, wherein organic silicon is extracted from rice hulls and compounded with nitrogen, phosphorus, potash fertilizers, trace fertilizers, humic acid, plant growth regulators and the like to prepare the foliar fertilizer. CN101768023A discloses an organic silicon fertilizer, and the raw material components and the weight percentage of the prepared organic agricultural fertilizer are as follows: oil shale powder raw material: 60% -70%, calcium superphosphate: 10% -15%, potassium sulfate: 10-15%, magnesium sulfate: 5 to 10 percent. CN103539512A discloses a special fertilizer for paddy rice, which contains 30-60% of humic acid and 40-70% of micro silicon powder. CN102875212A discloses an organic silicon fertilizer prepared by mixing alkaline residue with various plant residues, potassium nitrate, calcium superphosphate, animal wastes and the like, and composting and decomposing under the sunshine condition.
Disclosure of Invention
The invention aims to provide a preparation method of an organic silicon fertilizer, which is used for preparing a high-quality and high-efficiency composite silicon biological bacterial fertilizer with high absorption and utilization rate of crops.
The invention also aims to provide the organic silicon fertilizer which has high absorption and utilization rate of crops, high quality and high efficiency.
The invention also aims to provide a mixed bacterial liquid for preparing the organic silicon fertilizer.
The invention mainly utilizes the technology of microbial enzymolysis of silicon element to prepare a high-quality and high-efficiency composite biological organic silicon fertilizer series product which can be directly absorbed and utilized by crops.
Specifically, in one aspect, the invention provides a preparation method of an organic silicon fertilizer, which comprises the following steps:
a. silicate bacteria (Bacillus mucopolysaccharides), Aspergillus niger (Aspergillus niger), Bacillus licheniformis (Bacillus licheniformis), Bacillus subtilis (Bacillus subtilis), lactococcus lactis (Lactobacillus sublactantjs), Lactobacillus plantarum (Lactobacillus plantanum Holland) and Saccharomyces cerevisiae (Saccharomyces cerevisiae) are mixed according to the viable count of 30-35: 25-30: 8-10: 8-10: 8-10: 8-10: 13-15, fermenting for more than 15 days at 28-37 ℃ under anaerobic condition to prepare zymogen liquid with pH value of 4.0-3.5;
b. mixing the potassium feldspar and the zymocyte liquid obtained in the step a, wherein the mixing ratio of the potassium feldspar to the zymocyte liquid is as follows: the weight of the zymophyte liquid and the weight of the potassium feldspar are 2-6: 1;
c. and c, hermetically standing the mixed system obtained in the step b at the temperature of 28-37 ℃ for fermentation for more than 20 days to obtain the organic silicon fertilizer.
According to a specific embodiment of the present invention, in the step a, the silicate bacteria (Bacillus mucosus), Bacillus licheniformis (Bacillus licheniformis), and Bacillus subtilis (Bacillus subtilis) are commonly used in the field of microbial organic fertilizer, lactococcus lactis (lactolactis subs), lactobacillus plantarum (lactobacillus plantarum and Saccharomyces cerevisiae), and Saccharomyces cerevisiae (Saccharomyces cerevisiae) are commonly used in the field of food fermentation, and Aspergillus niger (Aspergillus niger) is commonly used in the field of soy sauce, vinegar food factories for producing amylase, protease, glucoamylase, and pectic acid. In addition, the strain can also optionally further comprise one or more of Streptomyces flavochraceus (Streptomyces silceus), photosynthetic bacteria, bacillus natto, potassium bacteria and the like, and the mixing proportion of the strains in the mixed strain liquid can be respectively as follows: the ratio of the number of live bacteria of silicate bacteria to one or more of streptomyces ochraceus, photosynthetic bacteria, bacillus natto, potassium bacteria and the like is 30-35: 1 to 10. The strains can be obtained commercially from China agricultural microbial protection center, the metabolism enzyme production performance during multi-strain culture is mainly utilized, specific requirements on specific strain characteristics are not required, and various strains such as the preservation numbers Accc10012, Accc11080, Accc11025, Accc40022, Accc11016 and the like can be purchased. The strains are common strains in the field, can be respectively activated and cultured according to the records of the prior art or the suggestions of strain suppliers to prepare seed liquid, and then are mixed for anaerobic fermentation according to the viable bacteria ratio.
In the invention, the strains belong to chemoautotrophic microorganisms with acidophilic heat resistance, strong fertility, fast metabolic frequency and high enzyme production activity. A large amount of active cell bodies rich in various biological enzymes are obtained through single-plant (aerobic) fermentation, and then are compounded according to different enzyme systems generated by different strains, and then are subjected to standing anaerobic fermentation, domestication and culture to promote cell fusion. Fermenting for more than 15 days, generally fermenting for 15-20 days, and detecting the pH value of the mixed system to be 4.0-3.5 to obtain the stable compound secondary metabolic energy of various biological enzymes as the seed solution of the enzymolysis agent.
Experiments prove that silicate bacteria can grow on nitrogen-free culture media such as potassium feldspar, apatite, powdered rock phosphate and the like, certain organic acid, amino acid and a large amount of biotin are generated by expanding fermentation, acid-producing protease, cellulase and glucoamylase are generated at the proper growth temperature of aspergillus niger at 37 ℃, the reproductive vitality of bacillus licheniformis and bacillus subtilis is extremely strong, the bacillus licheniformis can grow and reproduce in stone cracks, the stone cracks can be fully covered with moss once the temperature condition is proper, and the viable count of the bacillus subtilis is 4.21 × 10 within 24 hours after fermentation culture10cfu/ml. The biochemical strong composite fermentation of multiple strains is applied, the generation passage of enzyme is good, the enzymatic reaction speed is accelerated, and the conversion rate of zymolyte into products is higher.
In the present invention, the medium for culturing each strain may be referred to the description of the prior art or the suggestion of the strain supplier. The microorganism growth needs nutrients, and the common inorganic salts are mainly used: phosphorus, calcium, magnesium, sulfur + carbon source, nitrogen source. For example, the ratio of each component of the culture medium typical of silicate bacteria is as follows: k2HPO40.5g、CaCO31g、MgSO40.2g + 10g of mannitol, 0.4g of yeast extract and 1000ml of water; the typical culture medium of aspergillus niger comprises the following components in proportion: PDA- (charpy medium); the typical culture medium of the lichen and the bacillus subtilis comprises the following components in proportion: beef extract, peptone and NaCl each 5g, water 1000ml and the like.
According to the specific embodiment of the invention, in the preparation method of the organic silicon fertilizer, the single liquid culture of each strain and the compound anaerobic fermentation of multiple strains can also use soybean milk with 8-20% of solid content as a culture medium, or peptone, yeast extract or trace elements are further added into the soybean milk to meet the nutritional requirements of microorganism growth.
According to a specific embodiment of the present invention, the method for preparing the organic silicon fertilizer of the present invention, wherein the potash feldspar in step b is a stone or stone powder which is crushed to a particle size of less than 5cm × 5cm × 5cm in advance.
According to the specific embodiment of the invention, the potassium feldspar in the step b can be added into the fermentation system of the invention without sterilization, and can also be sterilized in advance, for example, the potassium feldspar is sterilized according to the following method:
washing potassium feldspar with clear water to remove floating dust, spraying 75% ethanol on the surface of the potassium feldspar to sterilize, placing the potassium feldspar in a closed environment to be sealed for more than 8 hours, taking out the potassium feldspar and standing the potassium feldspar to volatilize the ethanol, and thus obtaining the sterilized potassium feldspar.
According to the specific embodiment of the invention, in the preparation method of the organic silicon fertilizer, benzylaminopurine can be selectively added into the mixed system in the step b, and the addition amount of benzylaminopurine in the zymophyte liquid is preferably not more than 20mg/L of the zymophyte liquid.
According to the specific embodiment of the invention, after the step c and the step b are mixed, sealed and fermented for more than 20 days, the fermentation system is layered, the upper layer is suspension, the middle layer is colloid, and the bottom layer is sediment. Wherein the upper suspension is black brown silica gel liquid, the number of effective viable bacteria (cfu) is more than or equal to 2 hundred million/g, the number of coliform groups is less than or equal to 100/g, the death rate of ascarid eggs is more than or equal to 95 percent, and the indexes of the contents of heavy metals As, Ca, Pb, Cr and Hg meet the specification of NY/T798-2004. The colloid substance in the middle layer is a colloidal silica (brown yellow sticky substance), the number of effective viable bacteria (cfu) is more than or equal to 0.5 hundred million/g, the number of coliform groups is less than or equal to 100 per gram, the death rate of ascarid eggs is more than or equal to 95 percent, and the heavy metal content index conforms to the specification of NY/T798-2004. The sediment part of the bottom layer is siliceous slag (grey white), the effective viable count (cfu) is more than or equal to 0.2 hundred million/g, the coliform count is less than or equal to 100/g, the death rate of ascarid eggs is more than or equal to 95 percent, and the heavy metal content index accords with the specification of NY/884-. The three parts (upper suspension, intermediate colloidal matter and bottom sediment) of the fermentation system can be respectively used as biological organic silicon fertilizer, or other materials are further mixed to prepare the organic silicon fertilizer. Therefore, the preparation method of the organic silicon fertilizer comprises the following steps:
d. separating any one or more of the upper suspension, the intermediate colloidal substance and the bottom sediment of the fermentation system, and respectively using the separated upper suspension, intermediate colloidal substance and bottom sediment as the organic silicon fertilizer, or mixing two or three of the organic silicon fertilizers to prepare the organic silicon fertilizer, or further mixing one or more of the organic silicon fertilizers with other raw materials to prepare an organic silicon fertilizer product.
According to a specific embodiment of the invention, the preparation method of the organic silicon fertilizer can comprise at least one of the following steps:
d-1, separating the upper suspension of the fermentation system to prepare a liquid silicon fertilizer;
d-2, separating the intermediate colloid substance of the fermentation system to prepare powder silicon fertilizer;
d-3, separating the bottom sediment of the fermentation system to prepare the granular silicon fertilizer.
More specifically, the liquid silicon fertilizer is prepared according to the following method:
directly filtering and separating the upper layer suspension stock solution generated by the fermentation system to obtain the liquid silicon fertilizer;
or mixing 3-8 parts of humic acid and 0-8 parts of monopotassium phosphate with 90-95 parts of the upper suspension of the fermentation system, and standing and fermenting at 18-35 ℃ for 12-72 hours to obtain liquid, namely the liquid silicon fertilizer.
When the powder silicon fertilizer is prepared, the method can be carried out as follows:
mixing 10-30 parts of fly ash, 10-30 parts of humic acid, 0-6 parts of monopotassium phosphate and 0-3 parts of trace elements with 35-80 parts of intermediate colloid substances of the fermentation system to prepare a powder silicon fertilizer;
preferably, 20% of fly ash and 20% of humic acid are used as carriers to be mixed with 60% of intermediate colloidal substance of the fermentation system to prepare the powder silicon fertilizer.
When the granular silicon fertilizer is prepared, the following steps are carried out:
20-30 parts of humic acid, 20-30 parts of grass carbon, 5-15 parts of wormcast, 3-6 parts of clay and 0-8 parts of trace elements are mixed with 15-40 parts of bottom layer precipitates of the fermentation system, and the mixture is directly granulated or granulated after being fermented for 5-10 days at 18-35 ℃ under a closed condition to obtain granular silicon fertilizer;
preferably, 30% of humic acid, 30% of grass carbon, 15% of decomposed wormcast and 5% of clay are used as carriers to be mixed with 20% of bottom sediment of the fermentation system, and the mixture is directly granulated to obtain the granular silicon fertilizer.
On the other hand, the invention also provides an organic silicon fertilizer which is prepared according to the method. As mentioned above, the organic silicon fertilizer can be prepared by directly taking a fermentation product obtained by closed standing fermentation at 28-37 ℃ for 20-40 days, preferably 30-40 days in the step c as the organic silicon fertilizer; or separating any one or more of the upper suspension, the middle colloidal substance and the bottom sediment after the closed fermentation for more than 20 days in the step c, wherein the upper suspension, the middle colloidal substance and the bottom sediment are usually about 30-40 days, and respectively using the separated upper suspension, middle colloidal substance and bottom sediment as the organic silicon fertilizer or mixing two or three of the organic silicon fertilizers to prepare the organic silicon fertilizer; and c, after the fermentation is carried out in a closed manner for more than 20 days, one or more of the upper suspension, the middle colloidal substance and the bottom sediment are usually mixed with other raw materials to prepare the organic silicon fertilizer product, more specifically, the upper suspension of the fermentation system is separated and mixed with other raw materials to prepare a liquid silicon fertilizer, the middle colloidal substance of the fermentation system is separated and mixed with other raw materials to prepare a powder silicon fertilizer, and the bottom sediment of the fermentation system is separated and mixed with other raw materials to prepare a particle silicon fertilizer.
In addition, the invention also provides a zymogen liquid for preparing the organic silicon fertilizer, which is prepared according to the following method: silicate bacteria (Bacillus mucopolysaccharides), Aspergillus niger (Aspergillus niger), Bacillus licheniformis (Bacillus licheniformis), Bacillus subtilis (Bacillus subtilis), lactococcus lactis (Lactobacillus sublactantjs), Lactobacillus plantarum (Lactobacillus plantarii and Saccharomyces cerevisiae) and Saccharomyces cerevisiae (Saccharomyces cerevisiae) are added according to the viable count of 30-35: 25-30: 8-10: 8-10: 8-10: 8-10: 13-15, and fermenting for more than 15 days at 28-37 ℃ under anaerobic condition to prepare zymogen liquid with pH value of 4.0-3.5. The zymophyte liquid contains high-activity substances of specific enzymolysis silicon elements and is a biological enzymolysis liquid.
In conclusion, the invention takes the enzymolysis core technology as a breakthrough, promotes the high-efficiency conversion of biomass, and realizes the fundamental breakthrough of the biological enzymolysis mineral substance technology. According to the invention, the organic silicon fertilizer with the characteristics of good stability of effective components, high crop absorption rate, strong practicability, more water-soluble components and the like can be prepared by taking potassium feldspar as a raw material and utilizing a biological silicon enzymolysis technology. Experiments show that the organic silicon fertilizer is applied to various crops such as rice, wheat, corn, sugarcane, tobacco, peanut, fruit trees and the like, can induce eukaryotic cell division of crop seeds, enhances the biosynthesis capacity of rooting cells and proteins, and increases rooting points in the seed germination process, so that the moisture and the nutrient content in soil can be fully absorbed by the plants in the growing and young period, and a material foundation is laid for the later growth of the crops; promoting photosynthesis, enhancing chlorophyll synthesis ability, continuously forming carbohydrate survival reserve nutrients through leaves, and finally supplying the nutrients to fruits of plants.
The organic silicon fertilizer product is a synergist for activating and repairing soil and strengthening plant cell nutrition, and is a novel multifunctional fertilizer incomparable with other chemical fertilizers. The fertilizer can be used as a fertilizer to provide nutrients, can be used as a soil conditioner to improve soil, and has the functions of preventing diseases, preventing insects, degrading toxins and heavy metal pollution. The invention belongs to a unique invention and is determined to be a high-efficiency high-quality fertilizer for developing organic ecological agriculture by the outstanding characteristics of no toxicity, no smell, no deterioration, no loss, no public nuisance and the like.
Detailed Description
Technical features, objects, and advantages of the present invention will be more clearly understood and appreciated by those skilled in the art, and will be described in detail with reference to specific examples, which are included for the purpose of illustration only and are not intended to limit the scope of the present invention.
Example 1
Preparation of biological enzymolysis liquid
1. Selection of strains
a. Silicate bacteria (Bacillus mucopolysaccharides);
b. aspergillus niger (Aspergillus niger);
c. bacillus licheniformis (Bacillus licheniformis);
d. bacillus subtilis (Bacillus subtilis);
e. lactococcus lactis (lactococcus lactis subsp lactjs);
f. lactobacillus plantarum (lactobacillus plantarum Holland);
g. alcohol yeast (Saccharomyces cerevisiae).
All strains were purchased from the China agricultural microbial Protection center.
2. Performing single-strain aerobic fermentation on the strain, wherein the culture medium and the fermentation conditions refer to the suggestions of strain suppliers. Each strain can be fermented until the viable count reaches 5-10 hundred million/g or more than 5-10 hundred million/mL, and is used as seed liquid. Then, the seed liquid is prepared according to the viable count of silicate bacteria (Bacillus mucopolysaccharides), Aspergillus niger (Aspergillus niger), Bacillus licheniformis (Bacillus licheniformis), Bacillus subtilis (Bacillus subtilis), lactococcus lactis (Lactobacillus sublactatjs), Lactobacillus plantarum (Lactobacillus plantarum Holland) and Saccharomyces cerevisiae (Saccharomyces cerevisiae) of about 35: 30: 10: 10: 10: 10: 15, taking soybean milk with solid content of 10% as a culture solution, inoculating mixed bacteria liquid (the inoculation amount is about 5%), fermenting under the anaerobic condition at 28-30 ℃ for about 20 days to obtain acidic zymocyte liquid with the pH value of about 3.8, wherein the acidic zymocyte liquid is rich in various biological enzymes, has certain specificity on silicon enzymolysis elements and is high-activity biological enzymolysis liquid (for later use).
Treating potassium feldspar
1、K2O·Al2O3·6SiO2Molecular formulaThe main components are as follows: SiO 22、Al2O3、K2O、Na2O, CaO, is an alkali earth aluminosilicate mineral crystal structure, belonging to a framework structure.
2. Crushing the potassium feldspar into blocks with the size not larger than 5cm multiplied by 5cm, washing with clear water to remove floating dust, spraying 75% ethanol on the surface of the objects for disinfection, placing the objects in a closed container, sealing for more than 8 hours, taking out the objects, standing for 2 hours (naturally volatilizing the ethanol) to obtain the sterilized potassium feldspar, and placing the sterilized potassium feldspar in a vertical reaction container (the best glass body).
Third, enzymolysis process
1. Placing the sterilized potassium feldspar in a vertical fermentation container, and slowly adding the enzymatic hydrolysate according to a proportion of 5: 1 or 4: 1 (the measurement is properly adjusted according to the different silicon element contents of the potassium feldspar).
2. Sealing, fermenting, standing, catalyzing and decomposing at 28-30 ℃, wherein potassium feldspar can be observed to be suspended in liquid in 10-15 days, potassium feldspar dissolved substances are splashed bubbles in 15-20 days, the potassium feldspar starts to crack, the silica gel suspended substances are gradually immersed into the bacteria liquid, and the pH value is gradually increased.
3. Standing, carrying out double decomposition and sedimentation on an enzymatic reaction system for 7-10 days, layering a fermentation system to generate an upper suspension as a silica gel liquid, an intermediate complex as a silica gel body, and a bottom sediment as a silica residue.
Preparation of liquid silicon fertilizer
Filtering out the upper suspension (compound bacterial liquid) generated by enzymolysis and fermentation to obtain the liquid silicon fertilizer.
The main components are detected, the upper liquid part generated by the fermentation system is silica gel liquid (black brown), the pH value is 11 (alkaline), the number of effective viable bacteria (cfu) is more than or equal to 2 hundred million/g, the number of coliform groups is less than or equal to 100/g, the death rate of ascarid eggs is more than or equal to 95%, and the content indexes of heavy metals of As, Ca, Pb, Cr and Hg meet the NY/T798-2004 regulations.
The product is characterized in that: the product is a compound biological liquid silicon fertilizer which can be completely dissolved in water, the water solubility of the compound biological liquid silicon fertilizer is more than ten thousand times of that of silicon dioxide, and the compound biological liquid silicon fertilizer is the only silicon nutrient which can be directly absorbed and utilized by plants. The problem of difficult activity, water solubility and citrate solubility of the silicon element is fundamentally solved, and the silicon element is a single-element fertilizer with national standards. The method is mainly characterized in that:
(1) is suitable for foliage spraying, irrigation, flushing and topdressing. The nutrient is quickly absorbed, the fertilizer efficiency is good, the enzymatic silicon nutrient is in an ionic state, and the crop can be directly absorbed and utilized. The transport distance of nutrients to plants is shortened by the activity of microorganisms, and the utilization rate can reach 100%.
(2) The invention relates to a silicon-potassium compound biological fertilizer, which can meet the special fertilizer requirements of crops such as rice, wheat, sugarcane, corn, silicon-and potassium-loving fruit trees, vegetables and the like, can determine the proportion of nutrients according to the abundance condition of soil nutrients and the requirements of the crops on nutrient elements, determines the fertilizing amount and spraying times, and is non-toxic and harmless.
(3) The invention establishes a balanced compound system by using the liquid potassium silicate, the medium trace elements and the organic substances, can effectively improve the utilization rate of silicon and various nutrient elements of crops by scientific matching use, is favorable for improving the fertilizer efficiency, and is a novel fertilizer for spraying, irrigating and fertilizing and realizing the new technical field of water-fertilizer integrated agriculture.
The compound bacterial liquid fertilizer of the embodiment is sprayed on quartz sand soaked by Dobereiner nitrogen-free culture medium (25 g of quartz sand is placed in each dish), the nitrogen amount fixed after 5, 10 and 15 days is measured, and the bacterial liquid fixed alone is used as comparison, and the sample without any bacterial liquid sprayed is used as comparison. See table 1 below for results.
TABLE 1
The composite bacterial liquid and the spore-phosphorus-containing bacterial culture of the present example were inoculated into sterilized soil, and the change in the number of vegetative cells and spores of phosphorus bacteria was measured on a meat extract peptone agar plate after 15 days and nights of culture, and the results are shown in Table 2. And inoculating the non-sterilized soil into the composite bacterial liquid and the single phosphorus bacteria respectively, culturing at a proper temperature for 10 days by taking the non-inoculated soil as a control, measuring the water-soluble phosphorus content of each soil, and recording the result in a table 3.
TABLE 2
TABLE 3
The contrast is improved by 183.33 percent compared with the contrast and is improved by 30.8 percent compared with the phosphorus bacteria alone
Preparation of powder silicon fertilizer
The middle layer complex generated by the fermentation system is a colloidal silica (brown yellow sticky matter), contains 38 percent of effective silicon component, has the pH value of about 9.5 (alkalinity), the number of effective viable bacteria (cfu) is more than or equal to 0.5 hundred million/g, the number of coliform groups is less than or equal to 100/g, the death rate of ascarid eggs is more than or equal to 95 percent, and the index of heavy metal content accords with the specification of NY/T798-2004.
Mixing 20 parts of fly ash (100-mesh powder), 20 parts of humic acid (100-mesh powder) and 60 parts of middle-layer complex to obtain a mixture d, naturally drying at normal temperature (the water content is 30%), and sieving by using a 100-mesh vibrating screen to obtain a powder silicon fertilizer product.
The product is an organic silicon-potassium compound fertilizer with special molecular structure and obvious biological activity. The method is mainly characterized in that:
(1) is suitable for seed dressing, seedbeds, nutrition bowls and seedling raising, promotes mitosis of plant root cells, enhances photosynthesis and promotes biosynthesis of protein. The fertilizer can be applied to seedbeds and field base fertilizers according to a proper proportion, and is convenient to apply;
(2) the fertilizer is convenient for compounding of various nutrient elements, is used as a nutrient regulator and a soil conditioner, and continuously forms carbohydrate through root systems and leaves to store nutrients for crops;
(3) the content of effective silicon, humic acid and organic matters in soil is increased, diseases and insect pests caused by pesticides, herbicides and the like are effectively relieved and prevented, the physiological metabolism of various crops is promoted, the water channels of plants are increased, the yield is increased, and the quality is improved.
The powdery silicon fertilizer (multifunctional biological organic fertilizer) of the present example was used as a base fertilizer to perform experiments on soybean crops, and the results are shown in table 4.
TABLE 4
The significance is as follows: t 3.74 > t (001) ═ 2.97
The powder silicon fertilizer (multifunctional biological organic fertilizer) of this example was used to perform experiments on corn crops, and the results are shown in table 5.
TABLE 5
Preparation of granular silicon fertilizer
The bottom layer sediment generated by the fermentation system is siliceous residues (grey white), the pH value is about 9.5 (alkaline), the effective viable count (cfu) is more than or equal to 0.2 hundred million/g, the coliform count is less than or equal to 100/g, the death rate of ascarid eggs is more than or equal to 95 percent, and the index of the heavy metal content conforms to the specification of NY/884-.
30 parts of humic acid (100-mesh powder), 30 parts of grass carbon (100-mesh powder), 5 parts of clay (100-mesh powder), 15 parts of decomposed wormcast (100-mesh powder) and 20 parts of bottom-layer siliceous residues, and uniformly stirring by using a stirrer to obtain a mixture e;
and air-drying the silicon slag, crushing the silicon slag into 100 meshes, stirring the silicon slag and the mixture e, adding 3-5% of silica gel liquid according to the granulation requirement, and granulating, drying and screening to obtain a granular fertilizer product. The method is mainly characterized in that:
(1) the fertilizer is suitable for applying base fertilizer, seed fertilizer and additional fertilizer to field crops, is beneficial to the application of mechanical equipment and large-area fertilizer application operation, prevents the loss of N, P, K fertilizer and increases the fertilizer efficiency;
(2) the toxicity of heavy metals to crops and the pollution to soil are relieved, the mechanical strength of plant tissues is enhanced, and diseases and lodging resistance are achieved;
(3) the base fertilizer and the liquid fertilizer are applied, the spraying effect is better, the silicon element forms a siliceous layer from the root system to the stem leaves of the crops, the protective barrier of the plants to plant diseases and insect pests is enhanced, water is saved, drought resistance is improved, and the salt resistance of the plants is improved.
The rice was subjected to experiments using the granular silicon fertilizer (multifunctional bioorganic fertilizer) of this example, and the results are shown in table 6.
TABLE 6
The numbers in the table are the average of three replicates, the protability: f1.751.63 > F (0.01) ═ 9.78
The results of experiments on wheat using the granular silicon fertilizer (multifunctional bioorganic fertilizer) of this example are shown in table 7.
TABLE 7
Example 2
1. Selection of strains
a. Silicate bacteria (Bacillus mucopolysaccharides) Accc 10012;
b. aspergillus niger, commercially available from Harbin Zhengyang river soy sauce, vinegar food factory, a food grade Aspergillus niger useful in the factory for the production of enzyme preparations of amylase, protease, saccharifying enzyme, pectic acid;
c. bacillus licheniformis (Bacillus licheniformis) acc 11080;
d. bacillus subtilis Accc 11025;
e. lactococcus lactis (lactococcus lactis) Accc 11016;
f. lactobacillus plantarum (Lactobacillus plantarum Holland) P-8;
g. saccharomyces cerevisiae, a commonly used yeast in the beer preparation process, purchased from breweries;
h. potassium bacteria (potassium bacteria) are screened from tobacco rhizosphere soil according to the prior art, and are identified to have no significant difference with traditional potassium bacteria, and are cultured according to the traditional potassium bacteria.
2. Performing single-strain fermentation on the strains, wherein the culture medium and the fermentation conditions refer to the suggestions of strain suppliers. Each strain can be fermented until the viable count reaches 5-10 hundred million/g or more than 5-10 hundred million/mL, and is used as seed liquid. Then, the viable count of each seed liquid is about 30 according to the viable count of silicate bacteria (Bacillus mucoginosus), Aspergillus niger (Aspergillus niger), Bacillus licheniformis (Bacillus licheniformis), Bacillus subtilis (Bacillus subtilis), lactococcus lactis (Lactobacillus sublactatjs), Lactobacillus plantarum (Lactobacillus plantarum Holland), Saccharomyces cerevisiae (Saccharomyces cerevisiae), and Potassium bacteria (Bacillus subtilis): 25: 8: 8: 8: 8: 13: 8, taking soybean milk with solid content of 8 percent as a culture solution, adding about 2 percent of yeast extract, inoculating mixed bacteria liquid (the inoculum size is about 10 percent), fermenting under the anaerobic condition of 30 ℃ for about 20 days to obtain acidic zymogen liquid with the pH value of about 3.6, wherein the acidic zymogen liquid is rich in various biological enzymes, has certain specificity on silicon enzymolysis elements and is high-activity biological enzymolysis liquid (for standby).
3. Crushing the potassium feldspar into blocks with the size not larger than 5cm multiplied by 5cm, and adding the blocks into the high-activity biological enzymolysis liquid obtained in the step 2, wherein the ratio of the potassium feldspar to the enzyme liquid is 1: and 5, sealing and standing for fermentation at 30 ℃, wherein potassium feldspar can be observed to be suspended in the liquid on the 15 th day, potassium feldspar dissolved matters are in splash type bubbles on the 20 th day, the potassium feldspar starts to crack, the silica gel suspended matters are gradually immersed in the bacteria liquid, and the pH value is gradually increased.
Standing, carrying out double decomposition and sedimentation on an enzymatic reaction system for about 7 days, layering a fermentation system to generate an upper suspension as a silica gel liquid, an intermediate complex as a silica gel body, and a bottom sediment as a silica residue.
4. Preparation of liquid silicon fertilizer
Filtering out 95 parts of upper suspension produced by enzymolysis and fermentation, mixing with 5 parts of humic acid, standing and fermenting for 2 days at 28-30 ℃, and filtering out liquid, namely the liquid silicon fertilizer (compound bacterial liquid). Through detection, the upper liquid part generated by the fermentation system is silica gel liquid (black brown), the pH value is 11-13 (alkaline), the number of effective viable bacteria (cfu) is more than or equal to 2 hundred million/g, the number of coliform groups is less than or equal to 100/g, the death rate of ascarid eggs is more than or equal to 95%, and the content indexes of heavy metals of As, Ca, Pb, Cr and Hg meet the NY/T798-2004 regulations.
The composite bacterial liquid and the single potassium bacteria of the present example were inoculated into sterilized soil, and cultured at a suitable temperature for 15 days with no inoculation as a control, and the quick-acting potassium content in each soil was measured, and the results are recorded in table 8.
TABLE 8
5. Preparation of granular silicon fertilizer
The middle layer complex generated by the fermentation system is a colloidal silica (brown yellow sticky matter), contains effective silicon components of about 37.5 percent, has a pH value of about 10 (alkalinity), has an effective viable count (cfu) of more than or equal to 0.5 hundred million/g, has a coliform population of less than or equal to 100/g, has a ascarid egg death rate of more than or equal to 95 percent, and has a heavy metal content index meeting the NY/T798-2004 specification.
The bottom layer sediment generated by the fermentation system is siliceous residues (grey white), the pH value is about 10.5 (alkaline), the effective viable count (cfu) is more than or equal to 0.2 hundred million/g, the coliform count is less than or equal to 100/g, the death rate of ascarid eggs is more than or equal to 95 percent, and the index of the heavy metal content conforms to the specification of NY/884-.
10 parts of fly ash (100-mesh powder), 25 parts of humic acid (100-mesh powder), 45 parts of middle-layer complex and 20 parts of bottom-layer silicon residue are mixed, granulated, dried and sieved to obtain a granular fertilizer product.
The experiment of the tobacco leaves using the granular silicon fertilizer (multifunctional biological organic fertilizer) of this example was carried out, and the results are shown in table 9.
TABLE 9
The result shows that the multifunctional composite bacterial fertilizer can improve the yield of tobacco leaves and the quality of the tobacco leaves.
Claims (12)
1. A zymophyte liquid for preparing an organic silicon fertilizer is prepared according to the following method:
silicate bacteria (Bacillus mucopolysaccharides), Aspergillus niger (Aspergillus niger), Bacillus licheniformis (Bacillus licheniformis), Bacillus subtilis (Bacillus subtilis), lactococcus lactis (Lactobacillus sublactantjs), Lactobacillus plantarum (Lactobacillus plantanum Holland) and Saccharomyces cerevisiae (Saccharomyces cerevisiae) are mixed according to the viable count of 30-35: 25-30: 8-10: 8-10: 8-10: 8-10: 13-15, fermenting for more than 15 days at 28-37 ℃ under anaerobic condition to prepare zymogen liquid with pH value of 4.0-3.5;
wherein, the anaerobic fermentation uses soybean milk with solid content of 8-20% as a culture medium, or peptone, yeast extract or trace elements are further added into the soybean milk to meet the nutritional requirements of microbial growth.
2. A preparation method of an organic silicon fertilizer comprises the following steps:
a. preparing the zymogen liquid with the pH value of 4.0-3.5 according to claim 1;
b. mixing the potassium feldspar and the zymocyte liquid obtained in the step a, wherein the mixing ratio of the potassium feldspar to the zymocyte liquid is as follows: the weight of the zymophyte liquid and the weight of the potassium feldspar are 2-6: 1;
c. c, standing the mixed system obtained in the step b in a closed manner at the temperature of 28-37 ℃ for more than 20 days, and after the mixed system is fermented in a closed manner for more than 20 days, layering the fermented system, wherein the upper layer is suspension, the middle layer is colloid, and the bottom layer is precipitate;
d. separating any one or more of the upper suspension, the intermediate colloidal substance and the bottom sediment of the fermentation system, and respectively using the separated upper suspension, intermediate colloidal substance and bottom sediment as the organic silicon fertilizer, or mixing two or three of the organic silicon fertilizers to prepare the organic silicon fertilizer, or further mixing one or more of the organic silicon fertilizers with other raw materials to prepare organic silicon fertilizer series products.
3. The method as claimed in claim 2, wherein the potassium feldspar in the step b is stone and/or stone powder previously crushed to a grain size of less than 5cm x 5 cm.
4. The method according to claim 2 or 3, wherein the potassium feldspar in step b is a sterilized or non-sterilized potassium feldspar;
the potassium feldspar is sterilized according to the following method:
washing potassium feldspar with clear water to remove floating dust, spraying 75% ethanol on the surface of the potassium feldspar to sterilize, placing the potassium feldspar in a closed environment to be sealed for more than 8 hours, taking out the potassium feldspar and standing the potassium feldspar to volatilize the ethanol, and thus obtaining the sterilized potassium feldspar.
5. The method according to claim 2 or 3, wherein benzylaminopurine is added to the mixed system of step b.
6. The method of claim 2, comprising at least one of the following steps:
d-1, separating the upper suspension of the fermentation system to prepare a liquid silicon fertilizer;
d-2, separating the intermediate colloid substance of the fermentation system to prepare powder silicon fertilizer;
d-3, separating the bottom sediment of the fermentation system to prepare the granular silicon fertilizer.
7. The method of claim 6, wherein: when the liquid silicon fertilizer is prepared, the method comprises the following steps:
directly filtering and separating the upper layer suspension stock solution generated by the fermentation system to obtain the liquid silicon fertilizer;
or mixing 3-8 parts of humic acid and 0-8 parts of monopotassium phosphate with 90-95 parts of the upper suspension of the fermentation system, and standing and fermenting at 18-35 ℃ for 12-72 hours to obtain liquid, namely the liquid silicon fertilizer.
8. The method of claim 6, wherein: when the powder silicon fertilizer is prepared, the method comprises the following steps:
10-30 parts of fly ash, 10-30 parts of humic acid, 0-6 parts of monopotassium phosphate and 0-3 parts of trace elements are mixed with 35-80 parts of intermediate colloidal substances of the fermentation system to prepare the powder silicon fertilizer.
9. The method of claim 6, wherein: when the powder silicon fertilizer is prepared, the method comprises the following steps:
and mixing 20% of fly ash and 20% of humic acid serving as carriers with 60% of intermediate colloidal substances of the fermentation system to prepare the powder silicon fertilizer.
10. The method of claim 6, wherein: when the granular silicon fertilizer is prepared, the following steps are carried out:
20-30 parts of humic acid, 20-30 parts of grass carbon, 5-15 parts of wormcast, 3-6 parts of clay and 0-8 parts of trace elements are mixed with 15-40 parts of bottom layer precipitates of the fermentation system, and the mixture is directly granulated or granulated after being fermented for 5-10 days at 18-35 ℃ under a closed condition to obtain the granular silicon fertilizer.
11. The method of claim 6, wherein: when the granular silicon fertilizer is prepared, the following steps are carried out:
mixing 30% of humic acid, 30% of grass carbon, 15% of decomposed wormcast and 5% of clay serving as carriers with 20% of bottom-layer sediment of the fermentation system, and directly granulating to obtain the granular silicon fertilizer.
12. An organic silicon fertilizer prepared by the method of any one of claims 2 to 11.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511030420.5A CN106927941B (en) | 2015-12-31 | 2015-12-31 | Organic silicon fertilizer and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511030420.5A CN106927941B (en) | 2015-12-31 | 2015-12-31 | Organic silicon fertilizer and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106927941A CN106927941A (en) | 2017-07-07 |
| CN106927941B true CN106927941B (en) | 2020-09-04 |
Family
ID=59444274
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201511030420.5A Expired - Fee Related CN106927941B (en) | 2015-12-31 | 2015-12-31 | Organic silicon fertilizer and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106927941B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111909709B (en) * | 2020-09-04 | 2021-06-04 | 湖北理工学院 | Soil conditioner special for artemisia field and preparation method and application thereof |
| CN112174746A (en) * | 2020-10-09 | 2021-01-05 | 赢享(宁夏)管理咨询有限公司 | Production process of double-soluble water-soluble citrate-soluble porous structure composite silicon fertilizer |
| CN112939701A (en) * | 2021-04-20 | 2021-06-11 | 鹤壁亿丰科技有限公司 | Preparation method of bio-organic fertilizer |
| CN114349573A (en) * | 2022-01-26 | 2022-04-15 | 黑龙江鑫福垚科技发展有限公司 | Multifunctional high-silicon fertilizer prepared from Baoqing water-containing fly ash and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1290673A (en) * | 2000-11-03 | 2001-04-11 | 杜士强 | Method for producing bacterial potash fertilizer by microbial solid fermentation to decompose in-soluble potassium ore |
| CN101781152A (en) * | 2010-03-25 | 2010-07-21 | 北京精耕天下科技发展有限公司 | Multi-effect active silicon fertilizer and production process thereof |
-
2015
- 2015-12-31 CN CN201511030420.5A patent/CN106927941B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN106927941A (en) | 2017-07-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103848698B (en) | A kind of biological organic fertilizer utilizing natural pond slag aerobic fermentation to prepare and preparation method thereof | |
| CN103113167B (en) | Compound microbial organic fertilizer and preparation method thereof | |
| AU2008331378B2 (en) | Microorganisms, microbial phosphate fertilizers and methods for preparing such microbial phosphate fertilizers | |
| CN108130094B (en) | Sandy soil conditioner and application thereof | |
| CN116675574A (en) | Activation method of selenium-enriched ore and preparation method of bio-organic fertilizer | |
| CN101798241B (en) | Method for producing microbial organic fertilizer by using sludge from sewage plant | |
| CN1648107A (en) | Biological organic fertilizer and its preparing method | |
| CN102816006A (en) | Special apple microorganism bacterial manure and method for preparing same | |
| CN106045714A (en) | Organic fertilizer produced from tea seed meal and tea seed shells and preparing method thereof | |
| CN101463334A (en) | Fermentation liquor composition for preparing biological organic fertilizer and preparation and use thereof | |
| CN106927941B (en) | Organic silicon fertilizer and preparation method thereof | |
| KR102072900B1 (en) | manufacturing method of compost for salt removal | |
| CN113234616A (en) | Bacillus licheniformis, saline-alkali tolerant microbial agent and preparation method of biomass saline-alkali soil conditioner | |
| CN113321547A (en) | Rhizosphere growth promoting water-soluble microbial fertilizer and preparation method thereof | |
| CN103172416A (en) | Nitrile ester phosphorus potassium amine active fertilizer produced from organic wastes | |
| US20240051891A1 (en) | Plant material recycling inoculant and uses thereof | |
| CN112661580A (en) | Biological mineral fertilizer capable of improving saline-alkali soil and application thereof | |
| CN116240131B (en) | Composite microbial inoculant for promoting decomposition of corn straw and application thereof | |
| CN101759501B (en) | Complex microorganisms flora grain type ascharite fertilizer and preparation method thereof | |
| CN113234446A (en) | Biomass saline-alkali soil conditioner | |
| CN105693392A (en) | Biological organic fertilizer and preparation method and application thereof | |
| CN106916756A (en) | Fungal bacterial strain, microbial bacterial agent, stalk soil-repairing agent and its application | |
| CN1290673A (en) | Method for producing bacterial potash fertilizer by microbial solid fermentation to decompose in-soluble potassium ore | |
| CN114773113A (en) | Mushroom stick organic selenium biological fertilizer and preparation method thereof | |
| CN112538445B (en) | Preparation method and application of biological agent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200904 Termination date: 20211231 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |