CN112939701A - Preparation method of bio-organic fertilizer - Google Patents

Preparation method of bio-organic fertilizer Download PDF

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Publication number
CN112939701A
CN112939701A CN202110423884.1A CN202110423884A CN112939701A CN 112939701 A CN112939701 A CN 112939701A CN 202110423884 A CN202110423884 A CN 202110423884A CN 112939701 A CN112939701 A CN 112939701A
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China
Prior art keywords
organic fertilizer
carrier
strain
proportion
preparation
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Pending
Application number
CN202110423884.1A
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Chinese (zh)
Inventor
董文全
姜玉琴
高亚娟
叶恺
段清长
刘静
曾大庆
仝学平
张丽
魏钰馨
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Hebi Yifeng Technology Co ltd
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Hebi Yifeng Technology Co ltd
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Priority to CN202110423884.1A priority Critical patent/CN112939701A/en
Publication of CN112939701A publication Critical patent/CN112939701A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/50Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention discloses a preparation method of a biological organic fertilizer, which comprises the following steps: firstly, preparing an organic fertilizer carrier, uniformly mixing and stirring carbonized plants and farmyard manure according to the proportion of 1: 0.7-1, performing high-temperature sterilization treatment, adding strains, adding bacillus licheniformis into the organic fertilizer carrier according to the proportion of 100: 1-2, and mixing the materials according to the proportion of 100: 2.5-3.5, adding the strain of streptomyces ochraceus into the organic fertilizer carrier, uniformly stirring, uniformly stacking in the shade, carrying out self-heating fermentation, and turning over the organic fertilizer carrier every 2-4 days until the water content is below 30%. By adopting the method, the prepared organic fertilizer carrier is subjected to fermentation treatment, the content of infectious microbes in the carrier is low, the organic matter content is high, the organic fertilizer carrier is free of germs, weed seeds and the like after high-temperature treatment, soil organic matter can be effectively supplemented, and the organic fertilizer is a meta-acid fertilizer, so that the acid-base balance of soil can be improved, the growth of crops is promoted, and the yield is increased.

Description

Preparation method of bio-organic fertilizer
Technical Field
The invention relates to the technical field of preparation of biological organic fertilizers, in particular to a preparation method of a biological organic fertilizer.
Background
The biological organic fertilizer is a fertilizer which is compounded by microorganisms with specific functions and organic materials mainly taking animal and plant residues (such as livestock and poultry manure, crop straws and the like) as sources and undergoing harmless treatment and decomposition, has the effects of microbial fertilizer and organic fertilizer, has complete nutrient elements and provides comprehensive nutrients for crops; the biological organic fertilizer has high organic matter content, can improve soil, improve physical and chemical properties of the soil, enhance the water retention, fertilizer retention and fertilizer supply capabilities of the soil and relieve soil hardening caused by long-term use of chemical fertilizers; beneficial microorganisms in the bio-organic fertilizer enter soil and then form a mutual symbiotic proliferation relation with microorganisms in the soil, inhibit the growth of harmful bacteria and convert the harmful bacteria into beneficial bacteria, interact and mutually promote the beneficial bacteria to play a group synergistic effect, the beneficial bacteria generate a large amount of metabolites in the growth and reproduction process to promote the decomposition and conversion of organic matters, can directly or indirectly provide various nutrients and irritant substances for crops, and promote and regulate the growth of the crops. The biological organic fertilizer improves the porosity, permeability and exchangeability of soil and the survival rate of plants, and increases beneficial bacteria, soil microorganisms and populations. Meanwhile, the dominant beneficial flora formed on the root system of the crop can inhibit the propagation of harmful pathogenic bacteria, enhance the stress resistance and disease resistance of the crop, reduce the disease index of the continuous cropping crop, and greatly relieve continuous cropping obstacles after continuous application. The biological organic fertilizer recycles agricultural wastes, reduces environmental pollution, is safe and nontoxic to people, livestock and environment, and is an environment-friendly fertilizer.
But the content of mixed bacteria is high and the content of organic matters is low when the existing biological organic fertilizer is prepared.
Disclosure of Invention
The invention aims to provide a preparation method of a bio-organic fertilizer, which is used for solving the problems of high content of mixed bacteria and low content of organic matters in the existing preparation method of the bio-organic fertilizer.
In order to achieve the purpose, the invention provides the following technical scheme: a preparation method of a biological organic fertilizer comprises the following steps:
s1, preparing an organic fertilizer carrier, mixing and stirring the carbonized plants and the farmyard manure uniformly according to the proportion of 1: 0.7-1, and performing high-temperature sterilization treatment;
s2, adding strains, adding bacillus licheniformis into an organic fertilizer carrier according to a ratio of 100: 1-2, and mixing the bacillus licheniformis with the organic fertilizer carrier according to a ratio of 100: 2.5-3.5, adding the strain of streptomyces ochraceus into an organic fertilizer carrier, uniformly stirring, and uniformly stacking in a shade;
s3, self-heating fermentation, turning the organic fertilizer carrier once every 2-4 days until the water content reaches below 30%.
Preferably, the steps include:
s1, preparing an organic fertilizer carrier, mixing and stirring the carbonized plants and the farmyard manure uniformly according to the proportion of 1:1, and performing high-temperature sterilization treatment at the temperature of 80-90 ℃;
s2, adding strains, adding bacillus licheniformis into the organic fertilizer carrier according to the proportion of 100:1, adding the strains of streptomyces ochraceus into the organic fertilizer carrier according to the proportion of 100:3, uniformly stirring, and uniformly stacking in a shade place;
and S3, self-heating fermentation, turning over the organic fertilizer carrier once every 3 days until the moisture content reaches below 30 percent.
Preferably, the streptomyces ochraceus protobacteria are activated by a Gao's 1 culture medium, transferred to a triangular flask to serve as a liquid strain, and cultured for 4-5 days at the culture temperature of 28-30 ℃; performing solid fermentation, namely performing high-temperature sterilization by taking corn flour or bran as a carrier, inoculating 10 percent of the strain, keeping the water content of the material at 50 percent, culturing at the temperature of 30 ℃, discharging after 5-7 days of culture, naturally drying in the air, and crushing to obtain the streptomyces ochraceus strain.
Preferably, activating the bacillus licheniformis by a beef extract peptone culture medium, transferring the bacillus licheniformis to a triangular flask to serve as a liquid strain, and culturing for 3-4 days at the culture temperature of 28-33 ℃; performing solid fermentation, wherein the culture medium comprises corn flour, soybean meal, bran and the like, performing high-temperature sterilization, the inoculation amount is 7%, the material moisture is 60%, the culture temperature is 30 ℃, culturing for 4-5 days, discharging, naturally drying, and crushing to obtain the bacillus licheniformis strain.
Preferably, the bacteria content in the organic fertilizer reaches 0.8 hundred million/gram.
Preferably, in the step S1, the organic fertilizer carrier is sterilized at a high temperature of 65 ℃.
Compared with the prior art, the invention has the beneficial effects that:
by adopting the method, the prepared organic fertilizer carrier is subjected to fermentation treatment, the content of infectious microbes in the carrier is low, the organic matter content is high, the organic fertilizer carrier is free of germs, weed seeds and the like after high-temperature treatment, soil organic matter can be effectively supplemented, and the organic fertilizer is a meta-acid fertilizer, so that the acid-base balance of soil can be improved, the growth of crops is promoted, and the yield is increased.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.
The first embodiment is as follows:
a preparation method of a biological organic fertilizer comprises the following steps: firstly, preparing an organic fertilizer carrier, specifically, mixing and stirring carbonized plants and farmyard manure uniformly according to the proportion of 1:1, and performing high-temperature sterilization treatment at the temperature of 65 ℃, wherein germs can be inactivated at the moment, so that the quality of the organic fertilizer is ensured;
then adding a strain, cooling down an organic fertilizer carrier, adding bacillus licheniformis into the organic fertilizer carrier according to the proportion of 100:1, specifically, activating the bacillus licheniformis by a beef extract peptone culture medium, transferring to a triangular flask to serve as a liquid strain, culturing at the culture temperature of 28-33 ℃ for 3-4 days; performing solid fermentation, namely performing high-temperature sterilization on a culture medium comprising corn flour, soybean meal, bran and the like, wherein the inoculation amount is 7 percent, the material moisture is 60 percent, the culture temperature is 30 ℃, culturing for 4-5 days, discharging, naturally drying, crushing to obtain a bacillus licheniformis strain, and adding the bacillus licheniformis strain into an organic fertilizer carrier in a ratio of 100: 1.
Adding streptomyces flavochraceus strain into an organic fertilizer carrier according to the proportion of 100:3, specifically, activating streptomyces flavochraceus protobacteria by using a Gao's 1 culture medium, transferring the activated streptomyces flavochraceus protobacteria into a triangular flask to serve as a liquid strain, and culturing for 4-5 days at the culture temperature of 28-30 ℃; performing solid fermentation, namely performing high-temperature sterilization by taking corn flour or bran as a carrier, inoculating 10 percent of the corn flour or the bran, keeping the water content of the materials at 50 percent, culturing at 30 ℃, discharging after 5-7 days of culture, naturally drying in the air, crushing to obtain a strain of streptomyces ochraceus, and then adding the strain into an organic fertilizer carrier in a ratio of 100: 3.
And after the addition is finished, stirring, uniformly stacking the uniformly stirred organic fertilizer in a shade place, piling the organic fertilizer into a stack with the height of 1.2 meters, the width of about 2 meters, the length of the organic fertilizer is not limited, and lightly tapping the organic fertilizer tightly.
And finally, carrying out self-heating fermentation, turning the organic fertilizer once every 3 days, stacking the organic fertilizer into a pile with the height of 70-80 cm, the width of about 1.5 m, the length of the pile is not limited, and lightly and tightly beating the pile until the moisture content reaches below 30 percent, wherein the bacteria content in the prepared organic fertilizer reaches 0.8 hundred million/g.
The organic fertilizer carrier is fermented, the content of infectious microbes in the carrier is low, the carrier is free of germs, weed seeds and the like after high-temperature treatment, the content of organic matters is high, soil organic matters can be effectively supplemented, and the fertilizer is a slightly acidic fertilizer, so that the acid-base balance of soil can be improved, the growth of crops is promoted, and the yield is increased.
Example two:
a preparation method of a biological organic fertilizer comprises the following steps: firstly, preparing an organic fertilizer carrier, specifically, mixing and stirring carbonized plants and farmyard manure uniformly according to the proportion of 1:0.7, and performing high-temperature sterilization treatment at the temperature of 70 ℃, wherein germs can be inactivated at the moment, so that the quality of the organic fertilizer is ensured;
then adding a strain, cooling down an organic fertilizer carrier, adding bacillus licheniformis into the organic fertilizer carrier according to the proportion of 100:2, specifically, activating the bacillus licheniformis by a beef extract peptone culture medium, transferring to a triangular flask to serve as a liquid strain, culturing at the culture temperature of 28-33 ℃ for 3-4 days; performing solid fermentation, namely performing high-temperature sterilization on a culture medium comprising corn flour, soybean meal, bran and the like, wherein the inoculation amount is 7 percent, the material moisture is 60 percent, the culture temperature is 30 ℃, culturing for 4-5 days, discharging, naturally drying, crushing to obtain a bacillus licheniformis strain, and adding the bacillus licheniformis strain into an organic fertilizer carrier in a ratio of 100: 1.
Adding streptomyces flavochraceus strain into an organic fertilizer carrier according to the proportion of 100:2.5, specifically, activating streptomyces flavochraceus protobacteria by using a Gao's No. 1 culture medium, transferring the activated streptomyces flavochraceus protobacteria into a triangular flask to serve as liquid strain, and culturing for 4-5 days at the culture temperature of 28-30 ℃; performing solid fermentation, namely performing high-temperature sterilization by taking corn flour or bran as a carrier, inoculating 10 percent of the corn flour or the bran, keeping the water content of the materials at 50 percent, culturing at 30 ℃, discharging after 5-7 days of culture, naturally drying in the air, crushing to obtain a strain of streptomyces ochraceus, and then adding the strain into an organic fertilizer carrier in a ratio of 100: 3.
And after the addition is finished, stirring, uniformly stacking the uniformly stirred organic fertilizer in a shade place, piling the organic fertilizer into a stack with the height of 1.2 meters, the width of about 2 meters, the length of the organic fertilizer is not limited, and lightly tapping the organic fertilizer tightly.
And finally, carrying out self-heating fermentation, turning the organic fertilizer once every 3 days, stacking the organic fertilizer into a pile with the height of 70-80 cm, the width of about 1.5 m, the length of the pile is not limited, and lightly and tightly beating the pile until the moisture content reaches below 30 percent, wherein the bacteria content in the prepared organic fertilizer reaches 0.8 hundred million/g.
The organic fertilizer carrier is fermented, the content of infectious microbes in the carrier is low, the carrier is free of germs, weed seeds and the like after high-temperature treatment, the content of organic matters is high, soil organic matters can be effectively supplemented, and the fertilizer is a slightly acidic fertilizer, so that the acid-base balance of soil can be improved, the growth of crops is promoted, and the yield is increased.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Claims (6)

1. The preparation method of the bio-organic fertilizer is characterized by comprising the following steps:
s1, preparing an organic fertilizer carrier, mixing and stirring the carbonized plants and the farmyard manure uniformly according to the proportion of 1: 0.7-1, and performing high-temperature sterilization treatment;
s2, adding strains, adding bacillus licheniformis into an organic fertilizer carrier according to a ratio of 100: 1-2, and mixing the bacillus licheniformis with the organic fertilizer carrier according to a ratio of 100: 2.5-3.5, adding the strain of streptomyces ochraceus into an organic fertilizer carrier, uniformly stirring, and stacking in a shade;
s3, self-heating fermentation, turning the organic fertilizer carrier once every 2-4 days until the water content reaches below 30%.
2. The preparation method of the bio-organic fertilizer according to claim 1, characterized in that: the method comprises the following steps:
s1, preparing an organic fertilizer carrier, mixing and stirring the carbonized plants and the farmyard manure uniformly according to the proportion of 1:1, and performing high-temperature sterilization treatment at the temperature of 55-70 ℃;
s2, adding strains, adding bacillus licheniformis into the organic fertilizer carrier according to the proportion of 100:1, adding the strains of streptomyces ochraceus into the organic fertilizer carrier according to the proportion of 100:3, uniformly stirring, and stacking in a shade place;
and S3, self-heating fermentation, turning over the organic fertilizer carrier once every 3 days until the moisture content reaches below 30 percent.
3. The preparation method of the bio-organic fertilizer according to claim 2, characterized in that: activating Streptomyces ochrolensis protobacteria by using a Gao's 1 culture medium, transferring the activated Streptomyces ochrolensis protobacteria to a triangular flask to serve as a liquid strain, and culturing for 4-5 days at the culture temperature of 28-30 ℃; performing solid fermentation, namely performing high-temperature sterilization by taking corn flour or bran as a carrier, inoculating 10 percent of the strain, keeping the water content of the material at 50 percent, culturing at the temperature of 30 ℃, discharging after 5-7 days of culture, naturally drying in the air, and crushing to obtain the streptomyces ochraceus strain.
4. The preparation method of the bio-organic fertilizer according to claim 2, characterized in that: activating Bacillus licheniformis by beef extract peptone culture medium, transferring to triangular flask as liquid strain, culturing at 28-33 deg.C for 3-4 days; performing solid fermentation, wherein the culture medium comprises corn flour, soybean meal, bran and the like, performing high-temperature sterilization, the inoculation amount is 7%, the material moisture is 60%, the culture temperature is 30 ℃, culturing for 4-5 days, discharging, naturally drying, and crushing to obtain the bacillus licheniformis strain.
5. The preparation method of the bio-organic fertilizer according to claim 2, characterized in that: the bacteria content in the organic fertilizer reaches 0.8 hundred million/gram.
6. The preparation method of the bio-organic fertilizer according to claim 2, characterized in that: in step S1, the organic fertilizer carrier is sterilized at 65 ℃.
CN202110423884.1A 2021-04-20 2021-04-20 Preparation method of bio-organic fertilizer Pending CN112939701A (en)

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CN106927941A (en) * 2015-12-31 2017-07-07 高淑英 A kind of organic silicon fertilizer and preparation method thereof
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Application publication date: 20210611