CN102816006A - Special apple microorganism bacterial manure and method for preparing same - Google Patents
Special apple microorganism bacterial manure and method for preparing same Download PDFInfo
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- 244000005700 microbiome Species 0.000 title claims abstract description 25
- 210000003608 fece Anatomy 0.000 title claims abstract description 24
- 239000010871 livestock manure Substances 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims description 13
- 239000003337 fertilizer Substances 0.000 claims abstract description 38
- 239000002689 soil Substances 0.000 claims abstract description 25
- 241000894006 Bacteria Species 0.000 claims abstract description 17
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims abstract description 12
- 239000000618 nitrogen fertilizer Substances 0.000 claims abstract description 12
- 239000002686 phosphate fertilizer Substances 0.000 claims abstract description 12
- 229940072033 potash Drugs 0.000 claims abstract description 12
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- 235000015320 potassium carbonate Nutrition 0.000 claims abstract description 12
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- 239000004021 humic acid Substances 0.000 claims abstract description 9
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- 238000012216 screening Methods 0.000 claims abstract description 4
- 239000000417 fungicide Substances 0.000 claims description 19
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- 230000000855 fungicidal effect Effects 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 14
- 238000002360 preparation method Methods 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 10
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- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 239000002068 microbial inoculum Substances 0.000 claims description 6
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- 241000193830 Bacillus <bacterium> Species 0.000 claims description 4
- 241000194108 Bacillus licheniformis Species 0.000 claims description 4
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- 239000011575 calcium Substances 0.000 claims description 4
- 229910052791 calcium Inorganic materials 0.000 claims description 4
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- 238000010298 pulverizing process Methods 0.000 claims description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N urea group Chemical group NC(=O)N XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 4
- 239000005696 Diammonium phosphate Substances 0.000 claims description 3
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 3
- 238000010521 absorption reaction Methods 0.000 claims description 3
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical group [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 claims description 3
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 claims description 3
- 229910000388 diammonium phosphate Inorganic materials 0.000 claims description 3
- 235000019838 diammonium phosphate Nutrition 0.000 claims description 3
- 238000005469 granulation Methods 0.000 claims description 3
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- 235000019837 monoammonium phosphate Nutrition 0.000 claims description 3
- 239000011701 zinc Substances 0.000 claims description 3
- 229910052725 zinc Inorganic materials 0.000 claims description 3
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims description 2
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 claims description 2
- 230000003197 catalytic effect Effects 0.000 claims description 2
- 125000002091 cationic group Chemical group 0.000 claims description 2
- 229940047608 chelated magnesium Drugs 0.000 claims description 2
- 239000004927 clay Substances 0.000 claims description 2
- 239000006012 monoammonium phosphate Substances 0.000 claims description 2
- 229910021647 smectite Inorganic materials 0.000 claims description 2
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- 230000002522 swelling effect Effects 0.000 claims description 2
- 230000035558 fertility Effects 0.000 abstract description 3
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 abstract 1
- 239000000440 bentonite Substances 0.000 abstract 1
- 229910000278 bentonite Inorganic materials 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 238000003756 stirring Methods 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 12
- 238000012360 testing method Methods 0.000 description 7
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- 239000011591 potassium Substances 0.000 description 6
- 229910052700 potassium Inorganic materials 0.000 description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000011574 phosphorus Substances 0.000 description 5
- 229910052698 phosphorus Inorganic materials 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 4
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- 108010080698 Peptones Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 235000010724 Wisteria floribunda Nutrition 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
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- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
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- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
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Abstract
The invention discloses a special apple microorganism bacterial manure and relates to the technical field of microorganism bacterial manures. The microorganism bacterial manure takes 5-20 parts of compound microorganism bacterium agent, 10-20 parts of humic acid, 20-40 parts of turfy soil, 10-20 parts of nitrogenous fertilizer, 5-15 parts of phosphate fertilizer, 10-20 parts of potash fertilizer, 1-10 parts of micro element fertilizer and 1-10 parts of bentonite as raw materials, and is prepared by stirring, mixing, granulating, drying and screening. The special apple microorganism bacterial manure has the advantages of being complete in nutrition, improving apple yield and quality, improving fertility, prolonging fertilizer efficiency and improving soil environment.
Description
Technical field
The present invention relates to the microbial-bacterial fertilizer technical field, particularly relate to a kind of apple microbial bacterial manure special.
Background technology
Apple belongs to the Rosaceae, is deciduous tree, and stronger polarity is arranged, and it is vigorous to grow usually, and tree crown is tall and big, and the height of tree can reach 15 meters, under the cultivation condition about general high 3-5 rice.The trunk beige, Lao Pi has irregular lobe or scales off, and sprig is smooth.According to surveying and determination, every hectogram apple contains fructose 6.5-11.2 gram, glucose 2.5-3.5 gram, and sucrose 1.0-5.2 gram also contains trace element zinc, calcium, phosphorus, iron, potassium and VITMAIN B1, Wei ShengsuB2, vitamins C and Serlabo etc.Apple happiness light, happiness subacidity is to neutral soil, be suitable for most soil layer deep, be rich in organic matter, cubsoil is the well-drained sand of ventilation.
The mineral element that apple absorbed except forming output then, forms in addition and enough nourish and grow and reserve nutrient, in order to the needs that grow from now on.According to the apple orchard investigation of per mu yield more than 5000 kilograms, nitrogen phosphoris and potassium fertilizer ratio is 2:1:2, and 1000 kilograms of fruits of general every production need nitrogen 4-7 kilogram, phosphorus 2-3.5 kilogram, potassium 4-7 kilogram; Be main with nitrogen early stage, and the middle and later periods is main with potassium, and is more steady the whole year to the absorption of phosphorus.
Use chemical fertilizer to the production development of modern agriculture with ensure that human grain security established basic substance, yet the poor efficiency of chemical fertilizer is the global significant problem of puzzlement with the high ambient risk of bringing thus always.Therefore; Countries in the world are all in method and the approach trying to explore to improve utilization rate of fertilizer, check environmental pollution, and wherein microbial-bacterial fertilizer is a kind of environmental friendly fertilizer, also are one of important directions of the future of agriculture Sustainable development; Can keep and increase soil fertility; Help the breeding of mikrobe sociales, keep ecological balance, reduce environmental pollution etc.Also do not have the special microbial-bacterial fertilizer that design for apple in the prior art, the orchard worker is usually alternative with other composite fertilizer special, exist easily utilization rate of fertilizer low, apply fertilizer and cause soil compaction easily, influence problem such as apple quality year after year.
Summary of the invention
To the deficiency of prior art, the object of the present invention is to provide the apple microbial bacterial manure special that a kind of green non-pollution, utilization rate of fertilizer are high, improve the soil, meet the apple development nutritional needs.
The present invention realizes through following technical scheme:
Apple microbial bacterial manure special of the present invention, the feed composition weight part is following:
Complex micro organism fungicide 5-20 part,
Humic acids 10-20 part,
Turfy soil 20-40 part,
Nitrogenous fertilizer 10-20 part,
Phosphate fertilizer 5-15 part,
Potash fertilizer 10-20 part,
Little fertile 1-10 part,
Wilkinite 1-10 part;
Above raw material is the commercially available prod, wherein:
Described complex micro organism fungicide connects cultivation and fermentation by subtilis, gel-shaped bacillus, the independent expansion of Bacillus licheniformis; Bacterium liquid after the fermentation adsorbs with the peat composed of rotten mosses; With mixed, the pulverizing of the single microbial inoculum after the absorption according to 1:1:1; Moisture remains in 30%, gets complex micro organism fungicide, living bacteria count: 20,000,000,000/gram;
Described peat composed of rotten mosses wood water cut is 2-5%, organic content >=30%;
Said nitrogenous fertilizer is urea or an ammonium nitrate, and said phosphate fertilizer is monoammonium phosphate or diammonium phosphate, and said potash fertilizer is vitriolate of tartar;
Described humic acids water cut is 2-5%, organic content >=75%;
Described little fertilizer contains EDTA chelating calcium, EDTA chelated magnesium and EDTA chelating zinc;
Described wilkinite is to be main moisture clay pit with the smectite; Owing to have the special property of swelling property, cohesiveness, adsorptivity, catalytic, thixotropy, suspension and cationic exchange property; Be widely used in each industrial circle, be referred to as " omnipotent soil ";
The content that relates in the above raw material is mass percent.
The preparation method of apple microbial bacterial manure special of the present invention comprises following process step:
1) cultivation of single microbial inoculum
The bacterial classification that the test tube slant preserving process is preserved in 4 ℃ of refrigerators takes out; Expand and be connected on the fresh test tube beef extract-peptone slant medium; In 30 ℃ of incubators, cultivated ripe in 2-4 days; Be inoculated in the nutrient solution that is made up of Carnis Bovis seu Bubali cream 5g, peptone 5g, sodium-chlor 5g, water 1L, pH is 7.2, carries out shaking table and cultivates; Bacterium liquid bacterium number reaches the above entering one grade fermemtation stage of 2,000,000,000/milliliter, and the amount of seed liquor is a benchmark with the one grade fermemtation tank volume, and inoculum size is between 0.1%-10%; Adopt pressure differential method that seed liquor is pressed into fermentor tank, control pressure 0.05Mpa, 28 ℃ of temperature, the sterile air air flow is 0.5-1 a times of tank volume; After treating that one grade fermemtation finishes, directly bacterium hydraulic pressure is gone into the second order fermentation jar through pipeline, culture condition is the same; When fermented liquid reaches quality index pH is that 5.5-6.0, fermented liquid bacterium number reach 2,000,000,000/when milliliter is above, fermented liquid put into sterilized container for storing liquid; The aseptic peat composed of rotten mosses and the fermented liquid of water cut≤15%, fineness 80 mesh sieve percent of pass 80% are adsorbed with the ratio of 3-5:1, mix, controlling moisture content≤20%, living bacteria count>=200,000,000/gram get single microbial inoculum product.
2) complex micro organism fungicide preparation
The subtilis that will prepare respectively according to step 1 method, gel-shaped bacillus, Bacillus licheniformis are according to mixed, the pulverizing of 1:1:1, and moisture remains in 30%, complex micro organism fungicide, subsequent use.
3) preparation of product of the present invention
Branches such as above-mentioned raw materials complex micro organism fungicide, humic acids, turfy soil, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, little fertilizer, wilkinite another name is refitted in stirrer to mix 1.5 hours; Be under 8 the condition, to process the apple microbial bacterial manure special in 30 ℃ of temperature, pH value through granulation, oven dry, screening.
Apple microbial bacterial manure special of the present invention, further preferred feed composition weight part is following:
Complex micro organism fungicide 6-15 part,
Humic acids 12-18 part,
Turfy soil 25-35 part,
Nitrogenous fertilizer 12-18 part,
Phosphate fertilizer 6-10 part,
Potash fertilizer 12-18 part,
Little fertile 3-8 part,
Wilkinite 3-8 part.
Apple microbial bacterial manure special of the present invention, optimum feed composition weight part is following:
10 parts of complex micro organism fungicides,
15 parts of humic acidss,
25 parts of turfy soils,
16 parts in nitrogenous fertilizer,
8 parts in phosphate fertilizer,
16 parts in potash fertilizer,
Little fertile 5 parts,
5 parts of wilkinites.
Apple microbial bacterial manure special of the present invention has the following advantages:
1, has the effect that presses down germ, disease resistance
Owing to after a large amount of probioticss add in the soil, around crop root, form the protection barrier, suppress growth, the breeding of harmful bacterium; Purify edatope; The cover crop root system suppresses tens kinds of fungies, bacterium and viral disease, the generation that alleviates soil-borne disease to greatest extent simultaneously.
2, comprehensive nutrition, collection nitrogen, phosphorus, potassium and micro-activation are one, and non-available nutrient is absorbed again; Build up fertility; Prolong fertilizer efficiency, reduce fertilizer amount, the physiological disturbance that can effectively prevent trace elements such as plant boron deficiency, calcium, iron, magnesium, sulphur, manganese to cause.
3, can improve edatope, can eliminate soil compaction, chessom, neutralization alkalescence, reduce soil alkaline.
Each set of dispense ratio of product of the present invention can carry out suitable adjustment according to the regulation of fertilizer requirement of concrete edaphic condition and crop species and different growing, to satisfy the needs of plant growth.
Embodiment
Through the embodiment form foregoing of the present invention is remake further detailed description below; But should this be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following embodiment, all technology that realizes based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1: preparation apple microbial bacterial manure special
The feed composition weight part is following:
10 parts of complex micro organism fungicides,
15 parts of humic acidss,
25 parts of turfy soils,
16 parts in urea,
8 parts of monoammonium phosphates,
16 parts of vitriolate of tartar,
Little fertile 5 parts,
5 parts of wilkinites.
The preparation method:
1) cultivation of single microbial inoculum
The bacterial classification that the test tube slant preserving process is preserved in 4 ℃ of refrigerators takes out, and expands to be connected on the fresh test tube beef extract-peptone slant medium, in 30 ℃ of incubators, cultivates ripe in 2-4 days; Be inoculated in nutrient solution (Carnis Bovis seu Bubali cream 5g; Peptone 5g, NaCl 5g, pH7.2; Water 1L), cultivate in 28 ℃ of 160rpm-180rpm shaking tables; Bacterium liquid bacterium number reaches more than 2,000,000,000/milliliter, is mature seed and gets into the one grade fermemtation stage; The amount of seed liquor is a benchmark with the one grade fermemtation tank volume, between the inoculum size 0.1%-10%, adopts pressure differential method that seed liquor is pressed into fermentor tank, control pressure 0.05Mpa, and 28 ℃ of temperature, the sterile air air flow is 0.5-1 a times of tank volume; After treating that one grade fermemtation finishes, directly bacterium hydraulic pressure is gone into the second order fermentation jar through pipeline, culture condition is the same; When fermented liquid reaches quality index pH5.5-6.0, fermented liquid bacterium number reaches 2,000,000,000/milliliter when above, and fermented liquid is put into sterilized container for storing liquid, and is subsequent use; The aseptic peat composed of rotten mosses (water cut≤15%, fineness 80 mesh sieve percent of pass 80%) and fermented liquid are adsorbed with the 3-5:1 suitable proportion, mix, controlling moisture content≤20%, living bacteria count>=200,000,000/gram get single microbial inoculum product.
2) complex micro organism fungicide preparation
The subtilis that will prepare respectively according to step 1 method, gel-shaped bacillus, Bacillus licheniformis are according to mixed, the pulverizing of 1:1:1, and moisture remains in 30%, complex micro organism fungicide, subsequent use.
3) preparation of product of the present invention
Place stirrer to mix 1.5 hours 10 jin of complex micro organism fungicides of above-mentioned raw materials, 15 jin of humic acidss, 25 jin of turfy soils, 16 jin of nitrogenous fertilizer, 8 jin of phosphate fertilizer, 16 jin of potash fertilizer, 5 jin of little fertilizer, 5 jin of wilkinites; Be under 8 the condition, to process the apple microbial bacterial manure special in 30 ℃ of temperature, pH value through granulation, oven dry, screening.
Embodiment 2: preparation apple microbial bacterial manure special
The feed composition weight part is following:
8 parts of complex micro organism fungicides,
16 parts of humic acidss,
27 parts of turfy soils,
17 parts in urea,
7 parts of diammonium phosphate,
17 parts of vitriolate of tartar,
Little fertile 4 parts,
4 parts of wilkinites.
Preparation technology and product method of use are with embodiment 1.
Comparative Examples: composite fertilizer 13-7-20 (Stanley Fertilizer Co.,Ltd's production and sales) 300kg/ mu+farm manure 300kg/ mu.
The effect of above embodiment 1,2 and Comparative Examples is following:
Test period: in March, 2011-October
The test place: orchard, Guia Hill town, Xixia City, Yantai, mu is planted 86 strains.
Experimental cultivar: Fuji
Supply examination soil: sandy loam, good permeability; Organic content is 1.1%, nitric nitrogen 14.3mg/kg, ammonia-state nitrogen 80.1mg/kg, available potassium 235.3mg/kg, rapid available phosphorus 33.9mg/kg, pH value 7.3.
Blank, conventional fertilizer application, embodiment 1, embodiment 2 totally 4 processing are established in test, and each handles triplicate.Wherein, blank processing 6 strains, other are treated to 12 strains.
The completely random design is adopted in each experimental design.
Different treatment sees the following form to the influence of output:
Can be found out that by last table compare with contrast, fertilising can significantly improve the output of Fuji apple, the conventional fertilizer application comparison is according to raising the output 8.8%, and embodiment 1 and embodiment 2 compare respectively according to raising the output 26.2%, 21.2%.The apple production of embodiment 1 is the highest, reaches 2902kg/ mu, handles than embodiment 2 and improves 4.2%, handles than conventional fertilizer application and improves 16.0%, and effect of increasing production is best.
Therefore, use apple microbial bacterial manure special of the present invention and handle the output that can significantly improve apple, should vigorously promote the use.
Claims (4)
1. apple microbial bacterial manure special is characterized in that the feed composition weight part is following:
Complex micro organism fungicide 5-20 part,
Humic acids 10-20 part,
Turfy soil 20-40 part,
Nitrogenous fertilizer 10-20 part,
Phosphate fertilizer 5-15 part,
Potash fertilizer 10-20 part,
Little fertile 1-10 part,
Wilkinite 1-10 part;
Above raw material is the commercially available prod, wherein:
Described complex micro organism fungicide connects cultivation and fermentation by subtilis, gel-shaped bacillus, the independent expansion of Bacillus licheniformis; Bacterium liquid after the fermentation adsorbs with the peat composed of rotten mosses; With mixed, the pulverizing of the single microbial inoculum after the absorption according to 1:1:1; Moisture remains in 30%, gets complex micro organism fungicide, living bacteria count: 20,000,000,000/gram;
Described turfy soil water cut is 2-5%, organic content >=30%;
Said nitrogenous fertilizer is urea or an ammonium nitrate, and said phosphate fertilizer is monoammonium phosphate or diammonium phosphate, and said potash fertilizer is vitriolate of tartar;
Described humic acids water cut is 2-5%, organic content >=75%;
Described little fertilizer contains EDTA chelating calcium, EDTA chelated magnesium and EDTA chelating zinc;
Described wilkinite is to be main moisture clay pit with the smectite; Owing to have the special property of swelling property, cohesiveness, adsorptivity, catalytic, thixotropy, suspension and cationic exchange property; Be widely used in each industrial circle, be referred to as " omnipotent soil ";
The content that relates in the above raw material is mass percent.
2. apple microbial bacterial manure special as claimed in claim 1 is characterized in that the feed composition weight part is following:
Complex micro organism fungicide 6-15 part,
Humic acids 12-18 part,
Turfy soil 25-35 part,
Nitrogenous fertilizer 12-18 part,
Phosphate fertilizer 6-10 part,
Potash fertilizer 12-18 part,
Little fertile 3-8 part,
Wilkinite 3-8 part.
3. according to claim 1 or claim 2 apple microbial bacterial manure special is characterized in that the feed composition weight part is following:
10 parts of complex micro organism fungicides,
15 parts of humic acidss,
25 parts of turfy soils,
16 parts in nitrogenous fertilizer,
8 parts in phosphate fertilizer,
16 parts in potash fertilizer,
Little fertile 5 parts,
5 parts of wilkinites.
4. the preparation method of apple microbial bacterial manure special as claimed in claim 3; Comprise following process step: get complex micro organism fungicide, humic acids, turfy soil, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, little fertilizer, wilkinite by the regulation proportioning; Place stirrer to mix 1.5 hours above raw material; Be under 8 the condition, to make the apple microbial bacterial manure special in 30 ℃ of temperature, pH value through granulation, oven dry, screening.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101560124A (en) * | 2009-05-27 | 2009-10-21 | 陕西省微生物研究所 | Composition and preparation method of compound fertilizer special for fruit tree |
| CN101759500A (en) * | 2010-01-05 | 2010-06-30 | 爱沃(北京)农业技术有限公司 | Mineral fertilizer containing complex microbial community and preparation method thereof |
| US20120085647A1 (en) * | 2008-08-18 | 2012-04-12 | The University Of Dundee | Apparatus and method for mineralising biological materials |
| CN102515927A (en) * | 2011-09-21 | 2012-06-27 | 周法永 | Drug effect type microbial fertilizer, preparation method thereof and application thereof |
| CN102633567A (en) * | 2012-04-11 | 2012-08-15 | 安徽瑞然生物药肥科技有限公司 | Active biological medical fertilizer special for apples |
-
2012
- 2012-09-10 CN CN201210330016.XA patent/CN102816006B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120085647A1 (en) * | 2008-08-18 | 2012-04-12 | The University Of Dundee | Apparatus and method for mineralising biological materials |
| CN101560124A (en) * | 2009-05-27 | 2009-10-21 | 陕西省微生物研究所 | Composition and preparation method of compound fertilizer special for fruit tree |
| CN101759500A (en) * | 2010-01-05 | 2010-06-30 | 爱沃(北京)农业技术有限公司 | Mineral fertilizer containing complex microbial community and preparation method thereof |
| CN102515927A (en) * | 2011-09-21 | 2012-06-27 | 周法永 | Drug effect type microbial fertilizer, preparation method thereof and application thereof |
| CN102633567A (en) * | 2012-04-11 | 2012-08-15 | 安徽瑞然生物药肥科技有限公司 | Active biological medical fertilizer special for apples |
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